C146-E224B LC/MS/MS Rapid Toxicology Screening

C146-E224B
LC/MS/MS Rapid Toxicology
Screening System Ver.2
LCMS-8040
Predetermined Analytical Conditions
Semi-Quantitative Results without Authentic Standards
This system provides MRM transitions, LC separation conditions, retention
times, spectral libraries, and report files, enabling rapid implementation of
a screening method for many important toxicological substances.
By incorporating established calibration curves, this screening system provides
laboratories the ability to perform semi-quantitative analysis without purchasing
dozens of costly standards.
Also included is an optimized sample preparation protocol for toxicology
screening based on QuEChERS*. From sample preparation to reporting, a total
solution for toxicology screening is provided.
Screening for 161 Compounds
Developed in conjunction with front-line researchers in the toxicology
field, the method analyzes several classes of substances. LCMS MRM
optimization has been performed for common drugs of abuse, hypnotics,
psychotropics, pharmaceuticals and other natural toxins.
Shimadzu’s Ultra-Fast MS technology enables triggered product ion
scans, which provide MS/MS data during MRM acquisition.
* QuEChERS (Quick, Easy, Cheap, Effective, Rugged, Safe) was developed by
Anastassiades et al in 2003 as a sample preparation method for residual pesticide
analysis in agricultural products.
HPLC Conditions
(x100,000)
Analytical Column : Kinetex XB-C18 (2.1 mmI.D. × 100 mmL., 2.6 μm)
(Phenomenex)
Guard Column
: SecurityGuard Ultra C18 2.1mmID (Phenomenex)
Mobile phase A
: 10 mmol/L Ammonium formate + 0.1 % Formic acid in Water
Mobile phase B
: 10 mmol/L Ammonium formate + 0.1 % Formic acid in Methanol
Flow rate
: 0.3 mL/min
Injection volume : 5 μL
4.0
Step
Time (min)
A (%)
B (%)
0
0
95
5
1
7.5
5
95
2
10
5
95
3
10.01
95
5
4
15
95
5
Alprazolam
Diazepam-d5
309.10>281.10(+)
309.10>205.10(+)
290.15>154.05(+)
290.15>198.20(+)
3.0
2.0
1.0
0.0
10.1
12.5
15.0
,QWHQ [
P]
LC/MS/MS Rapid Toxicology Screening System Ver.2
Compound Panel
Registered substances include 161 compounds of illicit drugs, Hypnotics, psychotropics, pharmaceuticals and others that are often analyzed in the forensic medicine
field
[IS] Diazepam-d5(for positive mode)
Caffeine
Estazolam
Mepivacaine
Promethazine
[IS] Phenobarbital-d5 (for negative mode)
Carbamazepine
Ethenzamide
Mequitazine
Propericiazine
7-Aminoclonazepam
Carpipramine
Ethyl loflazepate
Metformin
Propofol (neg)
7-Aminoflunitrazepam
Chlordiazepoxide
Etizolam
Methamphetamine
Quazepam
7-Aminonimetazepam
Chlorpheniramine
Fenitrothion(MEP)
Methomyl
Quetiapine
7-Aminonitrazepam
Chlorpromazine
Fludiazepam
Methylephedrine
Risperidone
8-Hydroxyetizolam (M-III)
Clobazam
Flunitrazepam
Methylphenidate
Ropivacaine
9-Hydroxyrisperidone
Clocapramine
Flurazepam
Mexazolam
Salicylic acid (neg)
Acetaminophen
Clomipramine
Fluvoxamine
Mexiletine
Sertraline
Acetylpheneturide
Clonazepam
Gabapentin
Mianserin
Sildenafil
Aconitine
Clotiazepam
Glibenclamide
Midazolam
Spiperone
Allylisopropylacetylurea
Cloxazolam
Gliclazide
Milnacipran
Sulpiride
alpha-Hydroxyalprazolam
Clozapine
Glimepiride
Mirtazapine
Tadalafil
alpha-Hydroxybrotizolam
Cocaine
Haloperidol
Morphine
Tandospirone
alpha-Hydroxymidazolam
Codeine
Haloxazolam
Mosapramine
Temazepam
alpha-Hydroxytriazolam
Colchicine
Hydroxyzine
Nemonapride
THC
Alprazolam
Desipramine
Ibuprofen
Nicotine
THC-COOH
Amitriptyline
Desmethylclotiazepam
Imipramine
Nimetazepam
Thiamylal (neg)
Amobarbital (neg)
Desmethyldiazepam
Ketamine
Nitrazepam
Timiperone
Amoxapine
Dextromethorphan
Lamotrigine
Nortriptyline
Tofisopam
Amphetamine
Diazepam
Levetiracetam
Olanzapine
Topiramate
Aripiprazole
Diclofenac
Levomepromazine
Oxazepam
Trazodone
Atropine
Dihydrocodeine
Lidocaine
Paliperidone
Triazolam
Barbital (neg)
Diltiazem
Lorazepam
Paroxetine
Trihexyphenidyl
Benzoyl ecgonine
Diphenhydramine
Lormetazepam
Pemoline
Vardenafil
Biperiden
Diprophyline
Loxoprofen (neg)
Pentazocine
Valproic Acid (neg)
Blonanserin
Diquat
Malathion
Pentobarbital (neg)
Warfarin
Bromazepam
Donepezil
Maprotiline
Perospirone
Zolpidem
Bromocriptine
Dosulepin
MDA
Perphenazine
Zopiclone
Bromovalerylurea
Duloxetine
MDMA
Phenobarbital (neg)
Zopiclone-N-oxide
Bromperidol
Ecgonine methyl ester
Medazepam
Pimozide
Zotepine
Brotizolam
Ephedrine
Mefenamic acid
Pioglitazone
Bupivacaine
Escitalopram
Memantine
Primidone
Remarks and Precautions
1. For Research Use Only. Not for Use in Diagnostic Procedures.
2. The information of calibration curve in this Method Package is created by LCMS-8040.
3. Any calculated concentrations obtained using this system are estimated values only.
Because true values may differ significantly due to sample status and system conditions,
the estimates obtained are not valid for legal or medical decisions. To obtain accurate
quantitative values, a validated method using appropriate standards and quality control
procedures must be used.
4. Shimadzu makes no warranty regarding the accuracy of information included in the
database or the usefulness of information obtained from using the database.
5. It is the user’s responsibility to adopt appropriate quality control tests using standard
samples to confirm qualitative and semi-quantitative information obtained with this
method package.
© Shimadzu Corporation, 2014