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AIM2015 Agenda - Cancer Research Laboratory
12th Annual Advanced Imaging Methods Workshop February 4 – 6, 2015 Wednesday, February 4th 7:30 AM 8:30 AM 8:45 AM 9:30 AM 10:00 AM 10:30 AM 10:45 AM 11:15 AM 11:45 AM 12:45 PM 1:00 PM 1:30 PM 2:00 PM 2:30 – 3:30 nd Registration, Coffee & Pastries (Berkeley City Club, 2 Floor Atrium & Venetian Room) Holly Aaron, CRL Molecular Imaging Center, University of California, Berkeley (BCC Ballroom) Welcome Fluorescent Lifetime Imaging Microscopy (FLIM) I Chair: Wolfgang Becker, Becker-‐Hickl GmbH ~All talks will be in the BCC Ballroom~ Wolfgang Becker, Becker-‐Hickl GmbH, Berlin, Germany Introduction to Time-‐correlated Single Photon Counting (TCSPC) Angelika Rueck, Institute of Laser Medicine, Ulm, Germany Investigation of cell metabolism by FLIM and PLIM Klaus Suhling, King’s College, London, England Wide-‐field time-‐correlated single photon counting PLIM and FLIM Short Break & Becker-‐Hickl Demo (Julia’s Lounge) Forster Resonant Energy Transfer (FRET) Chair: Wolfgang Becker, Becker-‐Hickl GmbH Ammasi Periasamy, WM Keck Center for Cellular Imaging, University of Virginia, Charlottesville What is FRET microscopy, Why need it? Carlos Cesar Lenz, Instituto de Física, UNICAMP, Campinas, Brazil Analytical multi-‐modal platform with spatial, temporal and spectral resolution to perform linear and non-‐linear optical characterizations down to molecular level and 10 Kelvin Lunch for All (BCC Terrace & Member’s Lounge) Tools for Imaging: Filters and Probes Chair: Holly Aaron, CRL Molecular Imaging Center, UC Berkeley Alex Gao, Chroma Technology Corporation Choosing filters to optimize fluorescence imaging Richik Ghosh, Molecular Probes Engineering fluorescence to enhance information gathered from cells in multiple dimensions Fluorescent Lifetime Imaging II Chair: Wolfgang Becker, Becker-‐Hickl GmbH Thomas Gensch, Forschungszentrum Jülich, Germany Chloride imaging in the retina with fluorescence lifetime microscopy Alzbeta Chorvatova, International Laser Centre, Biophotonics, Slovakia Time-‐resolved autofluorescence images: from pixels back to photons Demos/Product Show – Berkeley City Club (Venetian Room & Julia’s Lounge) 3:00 PM 3:30 PM 4:00 PM 4:30 PM 5:00 PM 5:20 PM 5:50 PM Innovations I Chair: Mary West, QB3, UC Berkeley David Schaffer, University of California, Berkeley Molecular Elucidation and Engineering of the Stem Cell Fate Decisions Enrico Gratton, University of California, Irvine Nanoscale diffusion in cells: the iMSD approach Xavier Darzacq, University of California, Berkeley Imaging transcription using single molecule live cell microscopy Sara Abrahamsson, Rockefeller University, New York Crack the Skye -‐ precise nanofabrication of diffractive Fourier optical devices to advance multifocus microscopy (MFM) for live 3D microscopy with larger imaging volumes and higher sensitivity Break & Becker-‐Hickl Demo (Julia’s Lounge) Probing Synaptic Circuits Chair: Jen-‐Yi Lee, CRL Molecular Imaging Center, UC Berkeley Viviana Gradinaru, California Institute of Technology, Pasadena Visualizing the Activity and Anatomy of Brain Circuits: Optogenetic Sensors and Tissue Clearing Approaches Linda Wilbrecht, University of California, Berkeley Have You Ever Seen a Rule? Using in vivo 2-‐photon Imaging to Capture a Structural Trace of Rule Learning in Synaptic Structures of the Frontal Association Cortex Short Break 6:20 PM 6:30 PM Keynote Lecture: Steven Chu, Stanford University Introduction: Ehud Isacoff, University of California, Berkeley Title TBA 8:00 PM Dinner on your own, or Speaker Dinner ____________________________________ Thursday, February 5th 7:30 AM 8:30 AM 9:00 AM 9:30 AM 10:00 AM nd Registration, Coffee & Pastries (BCC 2 Floor Atrium & Venetian Room) Fluorescent Lifetime Imaging (FLIM) III Chair: Wolfgang Becker, Becker-‐Hickl GmbH Yves Mely, University of Strasbourg, France Fluorescence Lifetime Imaging Microscopy to monitor HIV-‐1 protein oligomerization Dmitri Papkovsky, University College Cork, Ireland Monitoring of Oxygenation in 3D Tissue Models by PLIM-‐TCSPC Sandeep Pallikkuth, University of Tennessee Health Science Center, Memphis Dynamic Conformational Transitions of sarcoendoplasmic reticulum Ca-‐ATPase (SERCA) quantified by single-‐molecule FRET Hauke Studier, Becker-‐Hickl, GmbH, Berlin, Germany Imaging fast physiological processes by FLIM 10:20 AM 10:45 AM 11:15 AM 11:45 AM 12:15 PM 1:00 -‐ 3:00 2:30 PM 3:00 PM 3:30 PM 4:00 PM 4:30 PM 5:00 PM 5:30 PM 6:00 PM 6:30 PM 7:00 – 9:00 Break & Becker-‐Hickl Demo (Julia’s Lounge) Clinical and Immunology Studies Chair: Rik van Gorsel, Boston Electronics Michael Roberts, University of Queensland, Australia Using ex vivo and in vivo Multiphoton Imaging and FLIM to Define the Disposition of Drugs, Metabolites and Nanoparticles as Well as Their Effects on Cells Melissa Skala, Vanderbilt University, Nashville, Tennessee Fluorescence lifetime imaging of metabolic heterogeneity in cancer Kandice Tanner, National Institutes of Health, National Cancer Institute, Bethesda, Maryland Deconstructing tumor organogenesis Lunch, for All (BCC Terrace & Member’s Lounge) Break for Demos – On-‐ and Off-‐site Please See Demo List for Systems and Locations – Sign-‐up Required! Single Molecule Imaging and Nanoscopy Bruce Cohen, Molecular Foundry, Lawrence Berkeley National Laboratory, California Duncan McMillan, Carl Zeiss Microscopy, LLC The All New LSM 8 Family – Faster, More Sensitive and Superresolution Alberto Diaspro, University of Genoa, Italy Fluorescence Microscopy towards Nanoscopy 2.0 Naomi Ginsberg, University of California, Berkeley Seeing nanoscale structure in soft materials using ultrafast exciton dynamics and scintillating near-‐ fields Shimon Weiss, University of California, Los Angeles Inorganic voltage nanosensors Sunney Xie, Harvard University, Boston, Massachusettes Label Free Imaging for Biology and Medicine Break & Becker-‐Hickl Demo (Julia’s Lounge) Computation Chair: Glen MacDonald, University of Washington Kevin Eliceiri, University of Wisconsin, Madison Open Source Image Informatics for Multidimensional Live Cell Imaging Changhuei Yang, Biophotonics Laboratory, California Institute of Technology, Pasadena Fourier Ptychography – Using computation to address physical optical challenges Laura Waller, University of California, Berkeley Computational imaging for high-‐resolution 3D phase microscopy st Poster Night – Appetizers and Drinks (BCC Drawing Room, 1 Floor) Poster Chairs: Jen-‐Yi Lee, Rik van Gorsel, Glen MacDonald, Kevin Eliceiri ____________________________________ Friday, February 6th 7:30 AM 8:30 AM 9:00 AM 9:30 AM 10:00 AM 10:30 AM 10:45 AM 11:15 AM 11:45 AM 12:15 PM 1:00 -‐ 3:00 2:30 PM 3:00 PM 3:30 PM 4:00 PM 4:30 PM nd Registration, Coffee & Pastries (BCC 2 Floor Atrium & Venetian Room) Plant Microscopy Chair: Denise Schichnes, University of California, Berkeley Alex Hertle, University of California, Berkeley A chloroplast localized Sec14p-‐like protein controls thylakoid biogenesis Jake Brunkard, University of California, Berkeley Chloroplasts extend stromules in response to internal redox signals Simon Gilroy, Univeristy of Wisconsin, Madison FRETing Over Stress: Imaging Systemic Signaling in Plants Rachel Wang, Academia Sinica, Taipei, Taiwan Super-‐Resolution Imaging of Meiotic Homologous Pairing in Maize Using Structured Illumination Microscopy Short Break & BCC Demos (Julia’s Lounge) Innovations II Chair: Chris Gainer, Vision Sciences, UC Berkeley Ke Xu, University of California, Berkeley Rediscovering cells at the nanoscale through super-‐resolution microscopy and correlated electron microscopy Evan Miller, University of California, Berkeley Electrophysiology, Unplugged: Imaging Excitable Cells with Voltage Sensitive Dyes Audrey Ellerbee, Stanford University, California Optical Coherence Tomography: Emerging Techniques and Applications Lunch, for All (BCC Terrace & Member’s Lounge) Break for Demos – Off-‐site Please See Demo List for Systems and Locations – Sign-‐up Required! Imaging in Neuroscience I Chair: Annette Chan, San Francisco State University Inna Slutsky, Univeristy of Tel Aviv, Israel Hippocampal plasticity: from single synapse dynamics to Alzheimer's disease Scott Baraban, University of California, San Francisco Imaging neural networks during spontaneous seizures in larval zebrafish Daniela Kaufer, University of California, Berkeley Blood Brain Barrier Disruption Leading to the Development of Epilepsy – Filling in the Blanks David Kleinfeld, University of California, San Diego Blood Flow and Brain Vascular Dynamics: Optics Explaining fMRI? Short Break 4:45 PM 5:15 PM 5:45 PM Imaging in Neuroscience II Chair: Holly Aaron, University of California, Berkeley Amir Arbabi, California Institute of Technology, Pasadena Optical components thinner than a wavelength and their application for microscopy Wesley Legant, Janelia Farms, Howard Hughes Medical Institute, Virginia Lattice Light Sheet Microscopy: Imaging molecules, cells and embryos at high spatiotemporal resolution Steven Vogel, National Institutes of Health, Bethesda, Maryland Fluorescence Polarization and Fluctuation Analysis (FPFA): A method for simultaneously measuring fluorescence lifetime, time-‐resolved anisotropy, fluorophore concentration, molecular brightness, and lateral diffusion times 6:15 PM 6:45 PM 7:15 – 9:00 FLIM IV Chair: Wolfgang Becker, Becker-‐Hickl Karsten König, Saarland University & JenLab, GmbH, Germany Multiphoton Skin Tomography of Astronauts Alexander Jelzow, Becker-‐Hickl, GmbH, Berlin, Germany Time-‐domain fNIRS: Monitoring Human Brain Function by TCSPC Fond Farewell – Light Refreshments & Drinks (Members’ Lounge)