EOC Lab

Transcription

EOC Lab
Name
Date
Class
Lab A
40 minutes
Mitosis and Meiosis
During cellular reproduction, many changes occur in the nucleus of cells involving the
chromosomes. You could think about these changes as a set of choreographed moves like
you would see in a dance. In this lab, you will act out the moves that chromosomes make
during mitosis and meiosis in order to understand the steps that occur when cells
reproduce.
Ask a Question
How do chromosomes change and move during mitosis and meiosis?
Materials
pool noodles
Safety
Make Observations
1. Read and complete a lab safety form.
2. Form a cell nucleus with four chromosomes represented by students holding four
different colors of pool noodles.
Other students play the part of the nuclear membrane and form a circle around
the chromosomes.
chromosome with two sister chromatids.
4. Perform the following steps of mitosis.
a. During prophase, the nuclear membrane breaks apart, and the nucleolus
disappears.
b. In metaphase, duplicated chromosomes align in the middle of the cell.
c. The sister chromatids separate in anaphase.
d. In telophase, the nuclear membrane reforms around two daughter cells.
5. Repeat steps 2 and 3. Then perform the following steps of meiosis.
a. In prophase I, the nuclear membrane breaks apart, the nucleolus disappears, and
homologous chromosomes pair up.
b. In metaphase I, homologous chromosomes line up along the center of the cell.
c. During anaphase I, the pairs of homologous chromosomes separate.
d. In telophase I, the nuclear membrane reforms.
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Reproduction of Organisms
Copyright © Glencoe/McGraw-Hill, a division of The McGraw-Hill Companies, Inc.
3. The chromosomes duplicate during interphase. Each chromosome is copied, creating a
Name
Date
Class
Lab A continued
e. Each daughter cell now performs meiosis II
independently. In prophase II, the nuclear
membrane breaks down, and the nucleolus
disappears.
Remember to use scientific
methods.
f. During metaphase II, duplicated chromosomes
Ask a Question
align in the middle of the cell.
Make Observations
Form a Hypothesis
g. Sister chromatids separate in anaphase II.
Test your Hypothesis
h. In telophase II, the nuclear membrane reforms.
Analyze and Conclude
Form a Hypothesis
Communicate Results
6. Use your observations to form a hypothesis about
the results of an error in meiosis. For example, you
might explain the results of an error during anaphase I.
Test Your Hypothesis
Copyright © Glencoe/McGraw-Hill, a division of The McGraw-Hill Companies, Inc.
7. Perform meiosis again, incorporating the error you chose in step 6.
8. Compare the outcome to your hypothesis. Does your data support your hypothesis?
If not, revise your hypothesis and repeat steps 6–8.
Analyze and Conclude
9. Compare and Contrast How are mitosis and meiosis I similar? How are they different?
10.
The Big Idea What is the difference between the chromosomes in cells at the
beginning and the end of mitosis? At the beginning and end of meiosis?
Reproduction of Organisms
45
Name
Date
Class
Lab A continued
11. Critique How did performing cellular replications using pool noodles help you
understand mitosis and meiosis?
Communicate Your Results
Create a chart of the changes and movements of chromosomes in each of the steps in
meiosis and mitosis. Include colored drawings of chromosomes and remember to draw the
cell membranes.
Copyright © Glencoe/McGraw-Hill, a division of The McGraw-Hill Companies, Inc.
Lab Tips
• Figure out where the boundaries of your cell are before you start.
• Review the phases of mitosis and meiosis before beginning to act out how the
chromosomes move during each process.
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Reproduction of Organisms
Name
Date
Class
Lab B
40 minutes
Mitosis and Meiosis
During cellular reproduction, many changes occur in the nucleus of cells involving the
chromosomes. You could think about these changes as a set of choreographed moves
like you would see in a dance. In this lab, you will act out the moves that chromosomes
make during mitosis and meiosis in order to understand the steps that occur when cells
reproduce.
Ask a Question
How do chromosomes change and move during mitosis and meiosis?
Materials
pool noodles
Safety
Make Observations
1. Read and complete a lab safety form.
2. Form a cell nucleus with four chromosomes represented by students holding four
different colors of noodles. Other students play the part of the nuclear membrane
and form a circle around the chromosomes.
Copyright © Glencoe/McGraw-Hill, a division of The McGraw-Hill Companies, Inc.
3. The chromosomes duplicate during interphase. Each chromosome is joined by another
chromosome of the same color, creating a double-stranded chromosome.
4. Perform mitosis.
a. During prophase, the nuclear membrane breaks apart, and the nucleolus disappears.
b. In metaphase, duplicated chromosomes align in the middle of the cell.
c. The sister chromatids separate in anaphase.
d. In telophase, the nuclear membrane reforms around two daughter cells.
5. Repeat steps 2 and 3. Perform meiosis.
a. In prophase I, the nuclear membrane breaks apart, the nucleolus disappears, and
homologous chromosomes pair up.
b. In metaphase I, homologous chromosomes line up along the center of the cell.
c. During anaphase I, the pairs of homologous chromosomes separate.
d. In telophase I, the nuclear membrane reforms.
e. Each daughter cell now performs meiosis II independently. In prophase II, the
nuclear membrane breaks down, and the nucleolus disappears.
f. During metaphase II, duplicated chromosomes align in the middle of the cell.
Reproduction of Organisms
47
Name
Date
Class
Lab B continued
g. Sister chromatids separate in anaphase II.
h. In telophase II, the nuclear membrane reforms.
Form a Hypothesis
6. Use your observations to form a hypothesis about
the results of an error in meiosis. For example, you
might explain the results of an error during
anaphase I.
Remember to use scientific
methods.
Make Observations
Ask a Question
Form a Hypothesis
Test your Hypothesis
Analyze and Conclude
Communicate Results
Test Your Hypothesis
7. Perform meiosis, incorporating the error you chose in
step 6.
8. Compare the outcome to your hypothesis. Does your data support your hypothesis?
If not, revise your hypothesis and repeat steps 6–8.
9. Compare and Contrast How are mitosis and meiosis I similar? How are they different?
10.
The Big Idea What is the difference between the chromosomes in cells at the
beginning and the end of mitosis? At the beginning and end of meiosis?
11. Critique How did performing cellular replications using pool noodles help you
understand mitosis and meiosis?
48
Reproduction of Organisms
Copyright © Glencoe/McGraw-Hill, a division of The McGraw-Hill Companies, Inc.
Analyze and Conclude
Name
Date
Class
Lab B continued
Communicate Your Results
Copyright © Glencoe/McGraw-Hill, a division of The McGraw-Hill Companies, Inc.
Create a chart of the changes and movements of chromosomes in each of the steps
in meiosis and mitosis. Include colored drawings of chromosomes and remember to draw
the cell membranes.
Extension
Investigate some abnormalities that occur when mistakes are made during mitosis or meiosis.
Draw a chart of the steps of reproduction showing how the mistake is made. Write a short
description of the problems that result from the mistake.
Lab Tips
• Figure out where the boundaries of your cell are before you start.
• Review the phases of mitosis and meiosis before beginning to act out how the
chromosomes move during each process.
Reproduction of Organisms
49
Name
Date
Class
Lab C
Cell Reproduction
Directions: Use the information and data from the Lab Mitosis and Meiosis to perform this lab.
You have acted out the moves that chromosomes make during mitosis and meiosis. This
should help you understand the steps that occur when cells reproduce. Sometimes, during
prophase I of meiosis, parts of homologous chromosomes might cross over each other and
exchange sections of their chromatids. Crossing over results in new combinations of alleles
that increase genetic diversity. Design a procedure similar to the original lab to model how
crossing over occurs. You will need to replace the pool noodles with a material that can be
cut or broken safely.
Please note that you must complete Lab B before beginning Lab C. Have your teacher
approve your design and safety procedures before beginning your experiment.
Copyright © Glencoe/McGraw-Hill, a division of The McGraw-Hill Companies, Inc.
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Reproduction of Organisms