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Workshop on Techniques in Modeling Human Cancer in Mice
Workshop on Techniques in Modeling Human Cancer in Mice May 2-9, 2015 WORKSHOP SCHEDULE This schedule and is subject to change. Saturday, May 2, 2015 Location: Highseas Conference Center 4:30pm Registration and reception 5:30pm Dinner 6:30pm Orientation and Introductions Charles Wray, Ph.D., The Jackson Laboratory David Threadgill, Ph.D., Texas A & M University Richard Halberg, Ph.D., University of Wisconsin Laboratory Mouse – History, genetics, tools, nomenclature and more David Threadgill, Ph.D., Texas A&M University Sunday, May 3, 2015 Location: Highseas Conference Center 7:00am Breakfast 8:15am Course Evaluations Sharon LaBrie, M.S., University of Maine Center for Research and Evaluation 8:30am Organoid cultures and their utility Richard Halberg, Ph.D., University of Wisconsin 9:30am Van transports participants to GRB 3rd Floor Training Laboratory Location: GRB Training Laboratory Rev. 3/24/15 9:45am Mouse Biomethodology: Handling, restraint, mandibular blood collection, IP, sub-Q and tail vein injections Jennifer Corrigan, The Jackson Laboratory 11:45pm Lunch – Roscoe’s 12:45pm Necropsy (including harvesting of urogenital system) Ruth Sullivan, V.M.D, Ph.D., University of Wisconsin 1:45pm Preparation of organoid cultures Richard Halberg, Ph.D., University of Wisconsin 6:00pm Van transports participants back to Highseas Conference Center Location: Highseas Conference Center 6:30pm Dinner 7:30pm Pathology lecture Ruth Sullivan, V.M.D, Ph.D., University of Wisconsin Monday, May 4, 2015 Location: Highseas Conference Center 7:00am Breakfast 8:30am Modeling cancer in mice Richard Halberg, Ph.D., University of Wisconsin Models of colon cancer David Threadgill, Ph.D., Texas A&M University 10:30am Van transports participants to GRB 3rd Floor Training Laboratory Location: GRB Training Laboratory 10:45am Endoscopy techniques Richard Halberg, Ph.D., University of Wisconsin David Threadgill, Ph.D., Texas A&M University 12:30pm Lunch – Roscoe’s 1:30pm Dissection and tumor quantification Richard Halberg, Ph.D., University of Wisconsin David Threadgill, Ph.D., Texas A&M University 4:30pm Pathology Slide review 1 Ruth Sullivan, V.M.D, Ph.D., University of Wisconsin Rev. 3/24/15 5:30pm Van transports participants back to Highseas Conference Center Location: Highseas Conference Center 6:00pm Dinner 7:30pm Oncologist perspective of mouse models of human cancer Dusty Deming, M.D., University of Wisconsin Tuesday, May 5, 2015 Location: Highseas Conference Center 7:00am Breakfast 8:30am Models of lung cancer Katerina Politi, Ph.D.., Yale University Models of prostate and ovarian cancer Nigel Crawford, M.B., Ch.B., Ph.D., NHGRI 10:30am Van transports participants to GRB 3rd Floor Training Laboratory Location: GRB Training Laboratory 10:45am Lung preparation and injections Katerina Politi, Ph.D., Yale University 12:30pm Lunch – Roscoe’s 1:15pm Palpating for internal tumors, prostate preparation and injections Nigel Crawford, M.B., Ch.B., Ph.D., NHGRI Practice techniques and check organoid cultures 5:30pm Van transports participants back to Highseas Conference Center Location: Highseas Conference Center 6:00pm Dinner Wednesday, May 6, 2015 6:00am Optional Hike Location: Highseas Conference Center 7:00am Rev. 3/24/15 Breakfast 10:00am Imaging Modalities Ruth Sullivan, V.M.D, Ph.D., University of Wisconsin 11:00am Brain cancer models Kyuson Yun, Ph.D., The Jackson Laboratory 12:00pm Van transports participants to JAX Main Lobby 12:15pm Rotating Sessions -Group 1 = Mouse Ultrasound Demo, CT and Xenogen Imaging (FGB 44) ** Jennifer Ryan, B.S., The Jackson Laboratory -Group 2 = Lunch at Roscoe’s 1:30pm Switch Groups -Group 2 = Mouse Ultrasound Demo, CT and Xenogen Imaging (FGB 44) ** Jennifer Ryan, B.S., The Jackson Laboratory -Group 1 = Lunch at Roscoe’s **IMPORTANT NOTE: The mouse ultrasound demo, the CT and Xenogen imaging lecture will take place behind a barrier area (FGB 44). In order to participate in these sessions, individuals must not have contact with rodents outside of The Jackson Laboratory for 5 days prior to this session. Additionally, individuals must shower prior to coming to The Jackson Laboratory on this day, wear laundered clothes and, as with all laboratory sessions, must wear socks, closed toe shoes and have no exposed skin below the waist. Location: GRB Training Laboratory 2:45pm Break 3:00pm Rotating Sessions -Group 1 = Brain Injections via Stereotomy Ruth Sullivan, V.M.D, Ph.D., University of Wisconsin -Group 2 = Kidney capsule implantation, castration and ovariectomy Jennifer Corrigan, M.S., The Jackson Laboratory 4:15pm Switch Groups -Group 2 = Brain Injections via Stereotomy Ruth Sullivan, V.M.D, Ph.D., University of Wisconsin -Group 1 = Kidney capsule implantation, castration and ovariectomy Jennifer Corrigan, M.S., The Jackson Laboratory 5:30pm Van transports participants back to Highseas Conference Center Location: Highseas Conference Center 6:00pm Rev. 3/24/15 Dinner Thursday, May 7, 2015 Location: Highseas Conference Center 7:00am Breakfast 8:30am Models of Breast Cancer Amy Moser, Ph.D., University of Wisconsin Mammary metastasis models and use of cancer cell lines Doug Hurst, Ph.D., University of Alabama at Birmingham 10:30am Van transports participants to GRB 3rd Floor Training Laboratory 10:45am Dissection of mammary glands, whole mount preparation, whole mount analysis Amy Moser, Ph.D., University of Wisconsin Doug Hurst, Ph.D., University of Alabama at Birmingham 12:45pm Lunch – Roscoe’s 1:30pm Mammary gland palpations, tumor and fat pad collection, lung collection, lymph node collection Amy Moser, Ph.D., University of Wisconsin Doug Hurst, Ph.D., University of Alabama at Birmingham 3:15pm Fat pad clearing for mammary cell transplantation, orthotopic injections into the mammary fat pad Amy Moser, Ph.D., University of Wisconsin Doug Hurst, Ph.D., University of Alabama at Birmingham 4:45pm Pathology slide review 2 Ruth Sullivan, V.M.D, Ph.D., University of Wisconsin 5:45pm Van transports participants back to Highseas Conference Center Location: Highseas Conference Center 6:00pm Dinner 7:30pm Cancer Stem Cell Lecture TBA Friday, May 8, 2015 Location: Highseas Conference Center 7:00am Rev. 3/24/15 Breakfast 8:30am Tipping the Balance: Targeting Genomic Instability for Lymphoma and Leukemia Treatment Kevin Mills, Ph.D., The Jackson Laboratory PDX Lecture TBA Humanized mice for cancer research Lenny Shultz, Ph.D., The Jackson Laboratory 12:00pm Lunch – Highseas 12:45pm Van transports participants to GRB 3rd Floor Training Laboratory Location: GRB Training Laboratory 1:00pm Leukemia/Lymphoma Techniques (Palpation of lymph node tumors, tumor lymph node dissection, spleen dissection and splenocyte isolation, bone marrow isolation) Kristin Lamont, Ph.D., and Jane Branca, The Jackson Laboratory 2:00pm PDX model generation TBA 3:00pm Repeat or develop new techniques 5:30pm Van transports participants back to Highseas Conference Center Location: Highseas Conference Center 6:00pm Lobster Dinner – Highseas Conference Center 7:30pm Perspectives gained and needed Saturday, May 9, 2015 Breakfast and departures Rev. 3/24/15
