Guidelines for Equipment Usage

Transcription

Guidelines for HIR Central Facility: AKTA Purifier Plus 10
The AKTA Purifier Plus 10 versatile, modular liquid chromatography systems for fast and reliable
separations of proteins and peptides.
The Committee reserves the right to make any necessary changes to the guidelines from time to time and as
and when needed.
Location
The AKTA Purifier Plus 10 is at present located at the HIR building, central facility 2.
Operating Hours
The AKTA Purifier Plus 10 is available for service from 10 am to 5 pm, working weekdays. Advance
appointments are required. Time could be booked up to two weeks in advance.
Online Booking
Only qualified users will have access to the booking calendar. You must book online to utilize the machine.
Please plan your experiments ahead and make sure the facility can accommodate your schedule. The slots are
scheduled on a first-booked-first-served basis. Confirmation is done at the discretion of the officer in charge,
to maximize availability for the entire UM community. Please keep your appointments, and show up for them
promptly. User has to complete the experiments within the time slot allocated. Failing to do so, the officer has
the right to abort the experiment and vacate the machine for the next user. Cancellation of booking must be
made at least two working days before the actual equipment operation time. You will be billed for 50% of the
charges for late cancellations. Users will have to fill up a letter of intent available online.
Export of Data
If necessary, data can be saved using CD (supplied by the user) only.
Charges
The AKTA Purifier Plus 10 is FOC for UM-researcher and RM20 per run for non-UM researchers if
researcher bring their own columns. Columns are available for rent :
No
1
2
3
4
5
6
7
8
7
6
7
Columns
MONO Q 4.6/100 PE
MONO S 4.6/100 PE
Superdex 75 10/300 GL
Hitrap Desalting 5ml
HiTrap IEX selection kit 1ml
HiTrap Protein A HP, 1ml
HiTrap Protein G HP 1ml
HiTrap Heparin 1ml
HiTrap SP HP 5ml
HiTrap Q HP 5ml
Superdex 200 10/300 GL
Cat. #
17-5179-01
17-5180-01
17-5174-01
17-1408-01
17-6002-33
17-0402-03
17-0404-03
17-0406-01
17-1152-01
17-1154-01
17-5175-01
UM (per run)
25
25
25
5
5
10
10
5
5
5
25
Non-UM (per run)
100
100
100
20
20
40
40
20
20
20
50
If column spoiled due to mishandling by the user, user is responsible for the cost of replacing the column.
Discounted package is available for frequent user. RM1500 per year for unlimited access. This discounted
package is available only for UM researchers with priority for UM HIR grant holders. All buffer including
20% EtOH will not be provided. All charges are not inclusive of general reagents or consumables. The
Committee reserves the right to make any necessary adjustment to the charges when needed.
Contact persons
For further information, please contact the officer in charge Ms
(03-7967 7075, [email protected]), Dr. Ea Chee Kwee ([email protected])
Lim
Moo
Eng
Prerequisites
Users must have received training on this machine or demonstrate knowledge of the equipment before use.
User has to run the machine according to SOP. User will have to agree to and sign a letter of indemnity and
liability.
Guidelines for using the HIR Atomic Emission Spectrometry (AES)
The HIR Agilent 4100 MP-AES runs unattended without flammable or expensive gas supply. This unit
runs entirely on air, which means one can perform automated multi-element analyses while eliminating
flammable and expensive gases. This unit offers:

Powerful performance - The magnetically excited microwave plasma source provides detection
limits down to sub ppb levels.

Better reliability - The robust system is built for a range of industries including Toxicology
studies, Geochemistry, Food Testing and Agriculture, Energy and Fuels, and Environmental, and is
ideal for remote environments.
The Committee reserves the right to make any necessary changes to the guidelines from time to time
and as and when needed.
Location
The HIR AES is at present located at the HIR building Fume Hood Room at 1st Floor.
Operating Hours
The HIR AES is available for service from 10 am to 5 pm, working weekdays. Advance appointments
are required. Time could be booked up to two weeks in advance. No block booking is allowed.
Online Booking
Only qualified users will have access to the booking calendar. You must book online to utilize the
machine. The slots are scheduled on a first-booked-first-served basis. Confirmation is done at the
discretion of the officer in charge, to maximize availability for the entire UM community. Please keep
your appointments, and show up for them promptly. User has to complete the experiments within the
time slot allocated. Failing to do so, the officer has the right to abort the experiment and vacate the
machine for the next user. Cancellation of booking must be made at least one working day before the
actual equipment operation time. You will be billed for 50% of the charges for late cancellations. Users
will have to fill up a letter of intent available online.
Export of Data
Data has to be saved using CD only.
Charges
For half-day usage, it will be free-of-charge for UM researchers and RM400 for non-UM researchers.
For one-day usage, it will be RM50 for UM researchers, and RM800 for non UM researchers. The
Contact persons
For further information, please contact the science officer in charge Ms Lim Moo Eng (03-7967 7075,
[email protected]), Responsible officers are Dr. Chan Kok Gan (03-79674509, [email protected])
and Dr Ea Chee Kwee ([email protected]).
Prerequisites
Users must have received training on this machine or demonstrate knowledge of the equipment before
use. User has to run the machine according to SOP. User will have to agree to and sign a letter of
indemnity and liability.
Guidelines for using the HIR Bioanalyzer Expert 2100
The HIR Bioanalyzer Expert 2100 is a multi-user facility which provides fast and user friendly
instrumentation to researchers for analyzing nucleotide and protein’s concentration and purity.
All consumable (Reagent and Chip) shall be provide by user. Before and after usage of the
Bioanalyzer, user are responsible to clean the detector head with clean ultrapure water with the
particular chip.
The Committee reserves the right to make any necessary changes to the guidelines from time to
time and as and when needed.
Location
The HIR Bioanalyzer is located at Central Facility 2, Level 1, HIR Building
Operating Hours
The HIR Bioanalyzer is available for service from 10 am to 5 pm, working weekdays. Advance
appointments are required. Time could be booked up to two weeks in advance. No block booking
is allowed. Slots for usage are set at 30 min max for each sample including priming the chip. The
number of people that can be present from each user laboratory is limited to two, except by
specific pre-arrangement with the facility staff.
Online Booking
Only qualified users will have access to the booking calendar. You must book online to utilize
the machine. The slots are scheduled on a first-booked-first-served basis. Confirmation is done at
the discretion of the officer in charge, to maximize availability for the entire UM community.
Please keep your appointments, and show up for them promptly. User has to complete the
experiments within the time slot allocated. Failing to do so, the officer has the right to abort the
experiment and vacate the machine for the next user. Cancellation of booking must be made at
least two working days before the actual equipment operation time. You will be billed for 50%
of the charges for late cancellations. Users will have to fill up a letter of intent available online.
Export of Data
Charges
Each sample is chargeable at RM10 per sample for UM researchers and RM50 per sample for
non-UM researchers. For frequent users, 50 sample is available at a discounted rate of RM400
per package. This package is available only for UM researchers with priority for UM HIR grant
holders. The Committee reserves the right to make any necessary adjustment to the charges when
needed.
Contact persons
For further information, please contact the officer in charge Ms Lim Moo Eng (03-7967 7075,
[email protected]), Dr. Chan Kok Gan (03-79674509, [email protected])
Prerequisites
Users must have received training on this machine or demonstrate knowledge of the equipment
before use. User has to run the machine according to SOP. User will have to agree to and sign a
letter of indemnity and liability.
Guidelines for using the HIR BIOLOG Facility
The HIR BIOLOG Facility is a multi-user facility which provides advanced, comprehensive
automated high throughput phenotype microarray system for quantitative phenotypic cellular
assays with microbial cells and mammalian cells; integrated for automatic characterization and
identification technology of bacteria [aerobic and anaerobic], yeasts and filamentous fungi. The
Committee reserves the right to make any necessary changes to the guidelines from time to time
Location
The HIR BIOLOG Facility is at present located at the 1st Floor Centre HIR lab, HIR Building.
Operating Hours
The HIR BIOLOG Facility is available for service from 10 am to 5 pm, working weekdays.
Advance appointments are required. Time could be booked up to two weeks in advance. Strictly no
block booking is allowed. Slots for usage are set at three-hours each i.e. 10 am – 1 pm and 2 pm – 5
pm, or from 10 am - 5pm. The number of people that can be present from each user laboratory is
limited to two, except by specific pre-arrangement with the facility staff.
Online Booking
discretion of the officer-in-charge, to maximize availability for the entire UM community. Please
keep your appointments, and show up for them promptly. User has to complete the experiments
within the time slot allocated. Failing to do so, the officer has the right to abort the experiment and
vacate the machine for the next user. Cancellation of booking must be made at least two working
days before the actual equipment operation time. You will be billed for 50% of the charges for late
cancellations. Users will have to fill up a letter of intent available online.
Export of Data
Charges
It is chargeable at RM100.00 per day slot for UM researchers and RM400.00 per day slot for nonUM researchers.
For frequent users, a 10 slot-package (one day slot) is available at a discounted rate of RM800 per
package. This discounted package is available only for UM researchers with priority for UM HIR
grant holders. All charges are not inclusive of reagents, buffers and consumables, BIOLOG plates
etc. The Committee reserves the right to make any necessary adjustment to the charges when
needed.
Contact persons
For further information, please contact the officer in charge Ms Lim Moo Eng (03-79677075,
[email protected]), Dr. Chan Kok Gan ([email protected])
Prerequisites
Guidelines for using the HIR Biomark HD and C1 Single-Cell auto Prep System
The HIR Biomark HD and C1 Single-Cell auto Prep System facility is a multi-user facility which provides resarchers
with cutting edge Biomark HD and C1 Single-Cell auto Prep System facility for latest research in single-cell study.
The Committee reserves the right to make any necessary changes to the guidelines from time to time and as and
when needed.
Location
The HIR Biomark HD and C1 Single-Cell auto Prep System facility is at present located at Central Facility 2.2,
Level 2, HIR Building.
Operating Hours
The HIR Biomark HD and C1 Single-Cell auto Prep System facility is available for use from 9 am to 5 pm, working
weekdays. Weekend or holdiay booking requires a separate booking that user will have to inform by email the lab
officer and responsible officers. Advance appointment is required. Time could be booked up to 1 day in advance.
Walk-in usage will only be allowed provided there is no prior booking and in such instance, the user will still need
to do the booking. No block booking is allowed.
Online Booking
Only qualified users will have access to the booking calendar. You must book online to utilize the machine. Please
plan your experiments ahead and make sure the facility can accommodate your schedule. The slots are scheduled
on a first-booked-first-served basis. Confirmation is done at the discretion of the officer in charge, to maximize
availability for the entire UM community. Please keep your appointments, and show up for them promptly.
User has to complete the experiments within the time slot allocated. Failing to do so, the officer has the right to
abort the experiment and vacate the machine for the next user. Any users who have booked the instrument but
for whatsoever reasons do not turn up or exceeded the booking time by 30 min to use the instrument, it is
deemed that the instrument booking is cancelled and the next user will be able to use (provided proper booking
and all other procedures complied), also in such instances, the users who failed to turn up on time will still be
liable to pay the full amount of rental (if applicable).
Cancellation of booking must be made at least two working days before the actual equipment operation time.
User will be billed for 100% of the charges for late cancellations. Users will have to fill up a letter of indemnity
available online.
Export of Data
Charges
For three-hour (and/or shorter time) is RM100 and for 1 day (nine-to-five working hour) slot is available at
RM200 per day for UM researchers. Each three-hour slot is chargeable RM200 and RM400, respectively, per slot,
for non-UM researchers.
For frequent users, 10 days-package is available at a discounted rate of RM600 per package. This package is
available only for UM researchers with priority to UM HIR grant holders.
The Committee reserves the right to make any necessary adjustment to the charges when needed.
Contact persons
[email protected]), Dr Lim Phaik Em (03-79677180, [email protected]) and Dr. Tee Kok Keng
(03-79677833, [email protected]).
Prerequisites
Users must have received training on this machine or demonstrate knowledge of the equipment before use. User
has to run the machine according to SOP. User will have to agree to and sign a letter of indemnity and liability.
Guidelines for using the Bio-Rad CFX96 Real Time PCR Facility
The Bio-Rad CFX96
Real Time Facility is a multi-user facility which provides
instrumentation to researchers for qPCR etc.
Location
The Bio-Rad CFX96 Real Time Facility is located at Central Facility 2, Level 1, HIR building.
Operating Hours
The Bio-Rad CFX96 Real Time Facility is available for service from 9 am to 5 pm, working
weekdays. Advance appointments are required. Time could be booked up to two weeks in
advance. No block booking is allowed. The number of people that can be present from each
user laboratory is limited to two, except by specific pre-arrangement with the facility staff.
Online Booking
the machine. Please plan your experiments ahead and make sure the facility can accommodate
your schedule. The slots are scheduled on a first-booked-first-served basis. Confirmation is
done at the discretion of the officer in charge, to maximize availability for the entire UM
community. Please keep your appointments, and show up for them promptly. User has to
complete the experiments within the time slot allocated. Failing to do so, the officer has the
right to abort the experiment and vacate the machine for the next user. Cancellation of booking
must be made at least two working days before the actual equipment operation time. You will
be billed for 100% of the charges for late cancellations. Users will have to fill up a letter of
intent available online.
Export of Data
Charges
Each one hour is chargeable at RM10 for UM researchers and RM50 for non-UM researchers.
For frequent users, a 100 hour-package is avalaible at a discounted rate of RM950 and a 200
hour-package is avalaible at a discounted rate of RM1800 and a 500-hour-package is available
at RM3000. This discounted package is available only for UM researchers with priority for UM
HIR grant holders. All charges are not inclusive of reagents and consumables. The Committee
reserves the right to make any necessary adjustment to the charges when needed.
Contact persons
For further information, please contact the officer in charge, Ms Lim Moo Eng (03-79677075,
[email protected]), Dr. Ng Ching Ching (03-79675872) or Dr Ea Chee Kwee
([email protected])
Prerequisites
Guidelines for using the HIR Capillary Electrophoresis
The HIR Capillary electrophoresis (CE) is a high efficiency and resolution, rapid analysis
time, plus minimal sample and solvent requirements. It is an established technique in many
laboratories. User are responsible on bringing their own solvents, column, and sample vial for
each run.
The Committee reserves the right to make any necessary changes to the guidelines from time
to time and as and when needed.
Location
The HIR Capillary Electrophoresis is at present located at the Bilik Khas 3, Ground Floor of
HIR building.
Operating Hours
The HIR Capillary Electrophoresis is available for service from 10 am to 5 pm, working
advance. No block booking is allowed. Slots for usage are set at maximum 3 hours for each
usage.
Online Booking
Only qualified users will have access to the booking calendar. You must book online to
utilize the machine. The slots are scheduled on a first-booked-first-served basis. Confirmation
is done at the discretion of the officer in charge, to maximize availability for the entire UM
right to abort the experiment and vacate the machine for the next user. Cancellation of
booking must be made at least two working days before the actual equipment operation time.
You will be billed for 50% of the charges for late cancellations. Users will have to fill up a
letter of intent available online.
Export of Data
Charges
Each use is chargeable at RM50 per sample for UM researchers and RM100 per sample for
non-UM researchers. For frequent users, 50 slot is available at a discounted rate of RM1500
per package. This package is available only for UM researchers with priority for UM HIR
grant holders. The Committee reserves the right to make any necessary adjustment to the
charges when needed.
Contact persons
For further information, please contact the officer in charge Ms Lim Moo Eng (03-7967
7075, [email protected]), Dr. Chan Kok Gan (03-79674509, [email protected])
Prerequisites
Users must have received training on this machine or demonstrate knowledge of the
equipment before use. User has to run the machine according to SOP. User will have to agree
to and sign a letter of indemnity and liability.
Guidelines for using the Agilent Cary 60 UV-VIS Spectrophotometer
The HIR Cary 60 UV-VIS facility is a multi-user facility which can handle the widest variety of
sample sizes and types with liquid sampling accessories including fiber optic probes and
couplers, Peltier and water thermostatted single and multicell holders, temperature probes,
microvolume sampling cells and rapid mix accessory for stopped flow kinetics. For solid
samples, choose from solid sample holder, fiber optic reflectance probe and coupler and fixed
angle specular reflectance accessories (SRA).
Location
The HIR Cary 60 UV-VIS facility is at present located at Central Facility 1, Level 1, HIR
Building.
Operating Hours
The HIR Cary 60 UV-VIS facility is available for service from 9 am to 5 pm, working
advance. No block booking is allowed.
Online Booking
your schedule. The slots are scheduled on a first-booked-first-served basis. Confirmation is done
at the discretion of the officer in charge, to maximize availability for the entire UM community.
Export of Data
Data has to be saved using CD only (if acpplicable).
Charges
Both three-hour (and/or shorter time) and nine-to-five working hour slots are available gratis
(free-of-charge) for UM researchers. Each three-hour slot is chargeable RM100 and RM200 for
nine-to-five hour slots, respectively, for non-UM researchers.
The Committee reserves the right to make any necessary adjustment to the charges when
needed.
Contact persons
Prerequisites
Guidelines for using the Bio-Rad Chemidoc MP
The ChemiDoc MP system is a full-feature instrument for gel or western blot imaging. It is designed to
address multiplex fluorescent western blotting, chemiluminescence detection, and general gel
documentation applications. The Committee reserves the right to make any necessary changes to the
guidelines from time to time and as and when needed.
Location
The ChemiDoc MP system is located at Demo Teaching Lab, Ground Floor, HIR building.
Operating Hours
The ChemiDoc MP system is available for service from 9 am to 5 pm, working weekdays. Advance
appointments are required. Time could be booked up to two weeks in advance. No block booking is
allowed. The number of people that can be present from each user laboratory is limited to two, except
by specific pre-arrangement with the facility staff.
Online Booking
machine. Please plan your experiments ahead and make sure the facility can accommodate your
schedule. The slots are scheduled on a first-booked-first-served basis. Confirmation is done at the
machine for the next user. Cancellation of booking must be made at least two working days before
the actual equipment operation time. You will be billed for 100% of the charges for late cancellations.
Users will have to fill up a letter of intent available online.
Export of Data
Data has to be saved using CD or authorized USB-thumb drive only.
Charges
Each one hour is chargeable at RM10 for UM researchers and RM50 for non-UM researchers. For
frequent users, a 100 hour-package is avalaible at a discounted rate of RM950 and a 200 hour-package
is avalaible at a discounted rate of RM1800 and a 500-hour-package is available at RM3000. This
discounted package is available only for UM researchers with priority for UM HIR grant holders. All
charges are not inclusive of reagents and consumables. The Committee reserves the right to make any
necessary adjustment to the charges when needed.
Contact persons
[email protected]) or Dr. Lau Yee Ling (03-79674749) [email protected]
Prerequisites
before use. User has to run the machine according to SOP. User will have to agree to and sign a letter
of indemnity and liability.
Guidelines for using the HIR Chromatin Immunoprecipitation (ChiP) Automated System which
includes (a) Bioruptor & (b) SX-8G IP-Star automated System
The HIR Chromatin Immunoprecipitation (CHIP) Automated System which includes (a) Bioruptor & (b) SX-8G IPStar automated System facility is a multi-user facility which provides resarchers with cutting edge on ChiPSeq
preparation facility for latest research. The Committee reserves the right to make any necessary changes to the
Location
The HIR Chromatin Immunoprecipitation (CHIP) Automated System facility is at present located at Ground Floor,
HIR Building.
Operating Hours
The HIR Chromatin Immunoprecipitation (CHIP) Automated System facility is available for use from 9 am to 5 pm,
working weekdays. Weekend or holdiay booking requires a separate booking that user will have to inform by
email the lab officer and responsible officers. Advance appointment is required. Time could be booked up to 1
day in advance. Walk-in usage will only be allowed provided there is no prior booking and in such instance, the
user will still need to do the booking. No block booking is allowed.
Online Booking
availability for the entire UM community. Please keep your appointments, and show up for them promptly.
liable to pay the full amount of rental (if applicable).
available online.
Export of Data
Charges
FOC for Bioruptor. For SX-8G IP-STAR system, three-hour (and/or shorter time) is RM100 and for 1 day slot is
available at RM200 per day for UM researchers. Each three-hour slot is chargeable RM200 and RM400,
respectively, per slot, for non-UM researchers.
Contact persons
[email protected]), Dr Lim Phaik Em (03-79677180, [email protected]) Dr. Chan Kok Gan (03-79674509,
[email protected])
Prerequisites
Guidelines for using the HIR BioRad QX200 Droplet Digital PCR System
The HIR Droplet Digital PCR facility is a multi-user facility which provides resarchers with absolute
quantification of target DNA molecules using probe-based digital PCR applications.. The Committee reserves
the right to make any necessary changes to the guidelines from time to time and as and when needed.
Location
The HIR Droplet Digital PCR facility is at present located at Ground Floor, HIR Building.
Operating Hours
The HIR Droplet Digital PCR facility is available for use from 9 am to 5 pm, working weekdays. Weekend or
holdiay booking requires a separate booking that user will have to inform by email the lab officer and
responsible officers. Advance appointment is required. Time could be booked up to 1 day in advance. Walk-in
usage will only be allowed provided there is no prior booking and in such instance, the user will still need to do
the booking. No block booking is allowed.
Online Booking
scheduled on a first-booked-first-served basis. Confirmation is done at the discretion of the officer in charge,
to maximize availability for the entire UM community. Please keep your appointments, and show up for them
promptly.
User has to complete the experiments within the time slot allocated. Failing to do so, the officer has the right
to abort the experiment and vacate the machine for the next user. Any users who have booked the
instrument but for whatsoever reasons do not turn up or exceeded the booking time by 30 min to use the
instrument, it is deemed that the instrument booking is cancelled and the next user will be able to use
(provided proper booking and all other procedures complied), also in such instances, the users who failed to
turn up on time will still be liable to pay the full amount of rental (if applicable).
available online.
Export of Data
Charges
RM20 per run for UM researchers. RM50 per run for non-UM researchers.
For frequent users, 100 runs-package is available at a discounted rate of RM1000 per package. This package is
Contact persons
[email protected]), Dr Ng Ching Ching (03-79675628, [email protected]) and Dr. Ea Chee Kwee
(03-79677022-2587, [email protected]).
Prerequisites
liability.
1st Version
Guidelines for using the Chemostat (Fermenter)
The HIR Chemostat (fermenter) facility is a multi-user facility which provides resarchers with
chemostat (fermenter) for continuos fermenation or cell culturing. The Committee reserves the
right to make any necessary changes to the guidelines from time to time and as and when needed.
Location
The HIR Chemostat (fermenter) facility is at present located at Ground Floor, HIR Building.
Operating Hours
The HIR Chemostat (fermenter) facility is available for service from 9 am to 5 pm, working
weekdays. Advance appointments are required. Time could be booked up to two weeks in advance.
No block booking is allowed. But due to the nature of experiment involving chemostat (fermenter)
facility that can span >3 days, the user must state clearly that how long the experiments will require
to complete, which will be approved by HIR committee on case-by-case basis. The number of
people that can be present from each user laboratory is limited to two, except by specific
pre-arrangement with the facility staff.
Online Booking
discretion of the officer in charge, to maximize availability for the entire UM community. Please
Export of Data
Data has to be saved using CD only (if acpplicable).
Charges
Both three-hour (and/or shorter time) and nine-to-five working hour slots are available gratis (freeof-charge) for UM researchers. Each three-hour slot is chargeable RM100 and RM200,
For frequent users, 10 days-package is available at a discounted rate of RM100 per package. This
package is available only for UM researchers with priority to UM HIR grant holders.
Contact persons
Prerequisites
Guidelines for using the Fluorescence Microscope
The HIR Fluorescence Microscope facility is a multi-user facility which provides resarchers with
Fluorescence Microscope for fluoresence and related microscopy work. The Committee reserves
the right to make any necessary changes to the guidelines from time to time and as and when
needed.
Location
The HIR Fluorescence Microscope facility is at present located at 1st Floor, HIR Building.
Operating Hours
The HIR Fluorescence Microscope facility is available for service from 9 am to 5 pm, working
No block booking is allowed. The number of people that can be present from each user laboratory
is limited to two, except by specific pre-arrangement with the facility staff.
Online Booking
Export of Data
Charges
Contact persons
Prerequisites
Guidelines for using the HIR Agilent Gas Chromatography-Sherlock MIDI Microbial
Identification System
The Agilent Gas Chromatography-MIDI Microbial Identification System is a multi-user facility
that offers researchers with identification of bacteria based on analysis of cellular lipidic fraction
of bacteria such as Fatty Acid Methyl Esters (FAME). Determination of the FAMEs was
coupled with the use of Agilent Gas Chromatography hence it allows rapid bacterial
identification without the requirement of biochemical kits. All consumables and sample
preparations have to be provided by user. Users are responsible to make sure the machine is not
contaminated with their sample (especially bacteria extracts) after usage. User are adviced to
switch on the instrument 30 mins before use.
Location
The HIR GC-MIDI is at present located at the HIR building Bilik Khas 3.
Operating Hours
The HIR GC-MIDI is available for service from 10 am to 5 pm, working weekdays. Advance
appointments are required. Time could be booked up to two weeks in advance. No block
booking is allowed. Slots for usage are set at maximum 50 samples per usage.
Online Booking
the machine. The slots are scheduled on a first-booked-first-served basis. Confirmation is done
Export of Data
Charges
Each sample is chargeable at RM15 per sample for UM researchers and RM50 per sample for
non-UM researchers. For frequent users, 50 sample is available at a discounted rate of RM700
per package. This package is available only for UM researchers with priority for UM HIR grant
holders. The Committee reserves the right to make any necessary adjustment to the charges
when needed.
Contact persons
Prerequisites
Revised April 2015
Guidelines for Using the HIR Agilent 5975C GC/MSD Facility
Gas chromatography-mass spectrometry (GC-MS) is an instrument that separates chemical compounds in a mixture (GC
component) and identifies the separated compounds by their mass over charge ratio (MS component). GC-MS is used in a
wide range of applications including analysis of lipids and drug metabolites, environmental analysis and identification of
chemical compounds in a mixture. One of the advantages of GC-MS is its ablity to provide identification of chemical
compounds based on the mass spectra provided in the incorporated library.
The Committee reserves the right to review and make any necessary changes to the Guidelines as and when needed.
Brand and Model
Agilent 7890A GC System equipped with 5975C inert MSD Mass Spectrometer
Location: Bilik Khas 3, Level G, HIR Building
Operating Hours
The Agilent 5975C GC/MSD facility is available for service from 10.00am to 5.00pm, working weekdays. (Special
arrangement may be made for overnight or over weekend analysis, if necessary.) Advanced appointment (minimum 2 weeks
in advance) is required.
Sample Preparation Requirements
 The samples MUST BE ABLE TO VOLATILISE at temperatures below 300C. Thus, samples of total plant extracts,
untreated waste-water, crude oil, strong acid or base solutions, salts, metals and polymeric materials are not suitable to
be analysed by GC-MS.
 Samples must be prepared in guranteed grade (GR) or GC-MS grade solvents, preferably acetone, methanol or hexane.
Less preferable are halogenated solvents (e.g., chloroform, methylene chloride, etc.) and ethers (e.g., diethylether,
THF). DO NOT use water, DMF, or DMSO as solvent.
 The upper limit of sample concentration is 100.0 ppm in 0.5–1.5 mL. Overloading samples may cause damage to the
ion source in the MS component.
 Every sample must be filtered through a 0.45 µm membrane filter before injection into the GC-MS.
 Every sample must be placed in a GC-MS specified sample vial with the correct septum and cap, i.e., Agilent Part
Number 5182-0714 (Pack of 100 screw top vials) and Agilent Part Number 5182-0717 (Pack of 100 blue screw caps
and PTFE/red rubber septas).
Software Package
NIST 2011 Library, Deconvolution and Reporting software, Fiehn GC/MS Metabolomics RTL Library, MassHunter Data
Analysis for GCMS
Online Booking
Only qualified users will have access to the booking calendar. You must book online to use the equipment. The slots are
scheduled on a first-book first-serve basis. Confirmation is done at the discretion of the officer in charge, to maximize
availability for the entire UM community. Please keep your appointment and show up on time. Every user must complete the
experiments within the time slot allocated. Otherwise, the officer has the right to abort the experiment and vacate the machine
for the next user. Cancellation of booking must be made at least two working days before the actual equipment operation time.
Otherwise, for late cancellation, a user will be charged 50% of the cost of the booked session. Users will have to fill up a letter
of intent available online.
For all projects please consult with the Officers-in-charge prior to sample submission. We look forward to working with users
and meeting users’ expectations.
Export of Data: Data will be saved using CD/ DVD only.
1
2
3
Charges
The Committee reserves the right to revise the charges as and when needed.
UM Users
Non UM Users
Frequent UM Users
Sample
RM50.00/sample
RM265.00/sample
RM474.00/10 samples (Injection) or
analysis1,2,3
(Injection)
(Injection)
RM2,250.00/50 samples (Injection)
Excludes sample preparation and data analysis
Every user to provide an appropriate GC column other than the standard column, HP-5ms [J&W GC Column - (5% Phenyl)
methylpolysiloxane - Cat No. 19091S-433]
GC-MS specified sample vial to be provided by every user
Contact persons
For booking and to purchase a rental package, please contact the officer-in-charge, Ms Lim Moo Eng (03-7967 7075,
[email protected]). For technical advice, please contact Dr Lee Guan Serm (03-7967 2577, [email protected]) or
Dr. Loke Mun Fai ([email protected])
Prerequisites
User must have received training on this equipment or demonstrate knowledge of the equipment before use. User has to
run the equipment according to SOP. User will have to agree to and sign a letter of indemnity and liability.
Guidelines for using the Agilent GC-QTOF Mass Spectrometer
The HIR Agilent GC-QTOF Facility is a multi-user facility which provides advanced instrumentation to
researchers for volatile compound mass spectrometry analysis.
User must provide reference of journals, publication or application notes to confirm that the samples must be
amenable to GC techniques and reference to GC-QTOF methods such as suitable mobile phase, gradient
composition, types of GC columns and MS detector settings are available. Samples must go through proper
sample preparation or clean-up procedure prior to GC injection.
when needed.
Location
The HIR Agilent GC-QTOF Facility is at present located at HIR Building Ground Floor, Bilik Khas 3.
Operating Hours
The HIR Agilent GC-QTOF Facility is available for service from 9 am to 5 pm, working weekdays. Weekend
or holdiay booking requires a separate booking that user will have to inform by email the lab officer and
responsible officers. Advance appointment is required. Time could be booked up to 1 day in advance. Walk-in
usage will only be allowed provided there is no prior booking and in such instance, the user will still need to do
Online Booking
scheduled on a first-booked-first-served basis. Confirmation is done at the discretion of the officer in charge, to
maximize availability for the entire UM community. Please keep your appointments, and show up for them
promptly.
liable to pay the full amount of rental.
Cancellation of booking must be made at least one working day before the actual equipment operation time.
Users will be billed for the full charges for late cancellations. Users will have to fill up a letter of indemnity
available online.
Export of Data
Charges
Each three-hour slot is chargeable at RM100 per slot for UM researchers and RM1800 per slot for non-UM
researchers. For nine-to-five working hour, it is chargeable at RM200 per slot for UM researchers and RM3500
per slot for non-UM researchers.
For frequent users, 100 hour-package is available at a discounted rate of RM2000 per package and 200 hourpackage is avalaible at a discounted rate of RM3000. This package is available only for UM researchers with
priority for UM HIR grant holders.
Contact persons
[email protected]), Responsible officers are Dr Loke Mun Fai ([email protected]), Dr. Chan Kok Gan
([email protected]).
Prerequisites
Guidelines for using the HIR Ion Torrent Personal Genome NGS
The HIR Ion Torrent Personal Genome NGS facility is a multi-user facility which provides resarchers with
cutting edge NGS platform. The Committee reserves the right to make any necessary changes to the
Location
The HIR Ion Torrent Personal Genome NGS facility is at present located at 1st Floor, HIR Building.
Operating Hours
The HIR Ion Torrent Personal Genome NGS facility is available for use from 9 am to 5 pm, working
weekdays. Weekend or holdiay booking requires a separate booking that user will have to inform by email
the lab officer and responsible officers. Advance appointment is required. Time could be booked up to 1
day in advance. Walk-in usage will only be allowed provided there is no prior booking and in such instance,
the user will still need to do the booking. No block booking is allowed.
Online Booking
Please plan your experiments ahead and make sure the facility can accommodate your schedule. The slots
are scheduled on a first-booked-first-served basis. Confirmation is done at the discretion of the officer in
charge, to maximize availability for the entire UM community. Please keep your appointments, and show
up for them promptly.
User has to complete the experiments within the time slot allocated. Failing to do so, the officer has the
right to abort the experiment and vacate the machine for the next user. Any users who have booked the
(provided proper booking and all other procedures complied), also in such instances, the users who failed
to turn up on time will still be liable to pay the full amount of rental (if applicable).
Cancellation of booking must be made at least two working days before the actual equipment operation
time. User will be billed for 100% of the charges for late cancellations. Users will have to fill up a letter of
indemnity available online.
Export of Data
Charges
For 1 day (nine-to-five working hour slot) is available at RM200 per day for UM researchers and RM1,000
per day for non-UM researchers.
For frequent users, 10 days-package is available at a discounted rate of RM1,000 per package. This package
is available only for UM researchers with priority to UM HIR grant holders.
Contact persons
[email protected]), Dr Lim Phaik Em (03-79677180, [email protected]) and Dr. Chan Kok Gan (0379674509, [email protected])
Prerequisites
use. User has to run the machine according to SOP. User will have to agree to and sign a letter of indemnity
and liability.
Guidelines for using the HIR Isothermal Titration Calorimeter (ITC)
The HIR ITC allow characterization of biomolecular interactions by providing direct, label-free
measurements of binding affinity and thermodynamics. ITC provides valuable information about
binding interactions, molecular stability, protein folding, and enzyme kinetics. ITC measures all binding
parameters in a single label-free, in-solution experiment. Parameters include binding affinity (KD),
reaction stoichiometry (n), enthalpy (ΔH), and entropy (ΔS). ITC is useful for characterizing
biomolecular interactions and the study of the mechanism of the molecular interaction.
Key applications include:
 Quantitation of binding affinity
 Measurement of thermodynamics and active concentration
 Characterization of mechanism of action
 Confirmation of intended binding targets in small molecule drug discovery
 Determination of binding specificity and stoichiometry
 Validation of IC50 values during hit-to-lead
 Measurement of enzyme kinetics
Location
The HIR ITC is at present located at the HIR building, 1st Floor, Central Lab.
Operating Hours
The HIR ITC is available for service from 10 am to 5 pm, working weekdays. Advance appointments
are required. Time could be booked up to two weeks in advance. No block booking is allowed.
Online Booking
machine for the next user. Cancellation of booking must be made at least one working day before the
actual equipment operation time. You will be billed for 50% of the charges for late cancellations. Users
will have to fill up a letter of intent available online.
Export of Data
Charges
For half-day usage, it will be free-of-charge for UM researchers and RM800 for non-UM researchers.
For one-day usage, it will be RM50 for UM researchers, and RM1500 for non UM researchers. The
Contact persons
[email protected]), Responsible officers are Dr. Chan Kok Gan (03-79674509, [email protected])
and Dr Ea Chee Kwee ([email protected]).
Prerequisites
use. User has to run the machine according to SOP. User will have to agree to and sign a letter of
indemnity and liability.
Guidelines for using the Agilent 6490 LC/MS/MS Triple Quadrupole Mass Spectrometer
The HIR Agilent 6490 LC/MS/MS Facility is a multi-user facility which provides advanced
instrumentation to researchers for most sensitive quantification tool of targeted compounds with
reduce sample preparation.
User must provide reference of journals, publication or application notes to confirm that the
samples must be amenable to LC techniques and reference to LC/MS/MS methods such as suitable
mobile phase, gradient composition, types of LC columns and MS detector settings are available.
Samples must go through proper sample preparation or clean-up procedure prior to LC injection.
Location
The HIR Agilent 6490 LC/MS/MS Facility is at present located at HIR Building Ground Floor,
Bilik Khas 3.
Operating Hours
The HIR Agilent 6490 LC/MS/MS Facility is available for service from 9 am to 5 pm, working
weekdays. Advance appointments are required. Time could be booked up to 3 days in advance. No
block booking is allowed.
Online Booking
Export of Data
Charges
Each three-hour slot is chargeable at RM100 per slot for UM researchers and RM1500 per slot for
non-UM researchers. For nine-to-five working hour, it is chargeable at RM200 per slot for UM
researchers and RM3000 per slot for non-UM researchers.
For frequent users, 100 hour-package is available at a discounted rate of RM2000 per package and
200 hour-package is avalaible at a discounted rate of RM3000. This package is available only for
UM researchers with priority for UM HIR grant holders.
Contact persons
For further information, please contact the science officer in charge Ms Lim Moo Eng (03-7967
7075, [email protected]), Responsible officers are Dr Loke Mun Fai ([email protected]),
Dr. Chan Kok Gan ([email protected]).
Prerequisites
Guidelines for HIR Central Facility: Luminex 200
The Luminex 200 is a multi-user facility which provides advanced instrumentation of
multiplex bead-based fluoroanalyzer based on the xMAP technology.
Location
The Luminex 200 is at present located at Central Facility 1, Level 1, HIR building.
Operating Hours
The Luminex 200 is available for service from 9 am to 5 pm, working weekdays. Advance
booking is allowed. Slots for usage are set at 5 hours per run of a 96-well plate (including
time for calibration, warm-up, detection and analysis, maintenance and shutdown). The
Online Booking
utilize the machine. Please plan your experiments ahead and make sure the facility can
accommodate your schedule. The slots are scheduled on a first-booked-first-served basis.
Confirmation is done at the discretion of the officer in charge, to maximize availability for
the entire UM community. Please keep your appointments, and show up for them promptly.
User has to complete the experiments within the time slot allocated. Failing to do so, the
officer has the right to abort the experiment and vacate the machine for the next user.
Cancellation of booking must be made at least two working days before the actual equipment
operation time. You will be billed for 50% of the charges for late cancellations. Users will
have to fill up a letter of intent available online.
Export of Data
Charges
Each three-hour slot is chargeable at RM200 per slot for UM researchers and RM500 per slot
for non-UM researchers. For frequent users, a 10-slot package is available at a discounted
rate of RM1,200 per package. This discounted package is available only for UM researchers
with priority for UM HIR grant holders. All charges are not inclusive of reagents or
consumables. The Committee reserves the right to make any necessary adjustment to the
Contact persons
For further information, please contact the officer in charge, Ms Lim Moo Eng
(03-79677075, [email protected]), Dr. Tee Kok-Keng ([email protected]).
Prerequisites
Guidelines for using the HIR MacsQuant Flow Cytometry Facility
The HIR MacsQuant Flow Cytometry Facility is a multi-user facility which provides advanced
instrumentation to researchers for analyzing various types of cells according to their
measurable properties. The Committee reserves the right to make any necessary changes to
the guidelines from time to time and as and when needed.
Location
The HIR MacsQuant Flow Cytometry Facility is at present located at the Central Facility Lab
2, Level 1, HIR building.
Operating Hours
The HIR MacsQuant Flow Cytometry Facility is available for service 24 hrs/ 7 days. Advance
booking is allowed except by specific pre-arrangement with the facility staff.
Online Booking
the machine. The slots are scheduled on a first-booked-first-served basis. Confirmation is
booking must be made at least two working days before the actual equipment operation
time. You will be billed for 50% of the charges for late cancellations. Users will have to fill up
a letter of intent available online.
Export of Data
Data has to be saved using Thumb drive only.
Charges
Each hour is chargeable at RM50.00 per slot for UM researchers and RM200.00 per hour for
non-UM researchers. For frequent users, a 100 hours-package is available at a discounted
rate of RM4,000.00 per package and a 300 hours-package is available at a discounted rate of
RM8,000. This discounted package is available only for UM researchers with priority for UM
HIR grant holders. All charges are not inclusive of reagents, buffers and consumables. The
Committee reserves the right to make any necessary adjustment to the charges when
needed.
Contact persons
For further information, please contact the officer in charge Ms Lim Moo Eng
(03-79677075, [email protected]), Dr. Ea Chee Kwee ([email protected]), Dr. Ng
Ching Ching ([email protected])
Prerequisites
equipment before use. User has to run the machine according to SOP. User will have to
agree to and sign a letter of indemnity and liability
Guidelines for using the Bruker MALDI-TOF Mass Spectrometer
The HIR Bruker MALDI TOF Mass Spectrometer facility is a facility which provides advanced
instrumentation to researchers working on biomarker discovery, oligonucleotides, small molecules,
polymer analysis and short peptide analysis. This instrument is useful for clinical proteomics,
functional genomics, small molecules, and microbiology. Samples must go through proper sample
preparation or clean-up procedure prior to analysis. The Committee reserves the right to make any
necessary changes to the guidelines from time to time and as and when needed.
Location
The HIR MALDI TOF Mass Spectrometer facility is at present located at HIR Building 1st floor,
Central Lab.
Operating Hours
The HIR MALDI TOF Mass Spectrometer facility is available for service from 9 am to 5 pm,
working weekdays. Advance appointments are required. Time could be booked up to 3 days in
advance. No block booking is allowed.
Online Booking
Export of Data
Charges
Each three-hour slot is chargeable at RM100 per slot for UM researchers and RM1500 per slot for
non-UM researchers. For nine-to-five working hour, it is chargeable at RM200 per slot for UM
researchers and RM3000 per slot for non-UM researchers.
For frequent users, 100 hour-package is available at a discounted rate of RM2000 per package and
200 hour-package is avalaible at a discounted rate of RM3000. This package is available only for
UM researchers with priority for UM HIR grant holders.
Contact persons
7075, [email protected]), Responsible officers are Dr Loke Mun Fai ([email protected]),
Dr. Chan Kok Gan ([email protected]).
Prerequisites
Guidelines for using the HIR Microarray System
The HIR Affymetrix microarray facility provides resarchers with cutting edge microarray for genes
expression profile work and related work. The Committee reserves the right to make any necessary
changes to the guidelines from time to time and as and when needed.
Location
The HIR micromanipulator facility is at present located at 1st Floor, HIR Building.
Operating Hours
The HIR microarray facility is available for use from 9 am to 5 pm, working weekdays. Weekend or holdiay
booking requires a separate booking that user will have to inform by email the lab officer and responsible
officers. Advance appointment is required. Time could be booked up to 1 day in advance. Walk-in usage
will only be allowed provided there is no prior booking and in such instance, the user will still need to do
Online Booking
Export of Data
Charges
For one day usage (eight-to-fve, and/or shorter time) is RM150 and 2 days slot is available at RM250 for
UM researchers; and RM500 and RM800, respectively, per slot, for non-UM researchers.
For frequent users, 10-day package is available at a discounted rate of RM800 per package. This package is
available only for UM researchers.
Contact persons
[email protected]), Dr Ea Chee Kwee (03-79675889, [email protected]) Dr. Chan Kok Gan (0379674509, [email protected])
Prerequisites
and liability.
Guidelines for using the HIR Micromanipulator
The HIR micromanipulator facility is a multi-user facility which provides resarchers with cutting edge
micromanipulator for latest micromanipulator related work. The Committee reserves the right to make any
necessary changes to the guidelines from time to time and as and when needed.
Location
The HIR micromanipulator facility is at present located at 1st Floor, HIR Building.
Operating Hours
The HIR micromanipulator facility is available for use from 9 am to 5 pm, working weekdays. Weekend or
responsible officers. Advance appointment is required. Time could be booked up to 1 day in advance.
Walk-in usage will only be allowed provided there is no prior booking and in such instance, the user will still
need to do the booking. No block booking is allowed.
Online Booking
Export of Data
Charges
For three-hour (and/or shorter time) is RM50 and for nine-to-five working hour slots are available at
RM150 per day for UM researchers. Each three-hour slot is chargeable RM100 and RM300, respectively,
per slot, for non-UM researchers.
Contact persons
[email protected]), Dr Lim Phaik Em (03-79677180, [email protected]) and Dr. Tee Kok Keng (0379677833, [email protected])
Prerequisites
and liability.
Revised Rate 27 Dec 2012 Guidelines for using the HIR Microplate Reader (M200 Pro)
The HIR Microplate Reader (M200 Pro) is a multi-user facility which provides advance
instrumentation to researchers to read the absorbance, luminescence and fluorescence at
different time frame. The measurement of absorbance, luminescence and fluorescence is fully
automated by the machine and hence it is a very easy to use machine. All consumable (96 or 384
well plate) shall be provide by user. User are responsible to make sure the machine is not
contaminated with their sample (especially bacteria sample) after usage. User are advice to
preset the temperature of the machine 15min before use.
Location
The HIR Microplate Reader (M200 Pro) is at present located at the Central Facility 2, Level 1,
HIR Building.
Operating Hours
The HIR Microplate Reader (M200 Pro) is available for service from 10 am to 5 pm, working
advance. No block booking is allowed. Slots for usage are set at maximum 3 days for each
usage.
Online Booking
Export of Data
Charges
Unit of Measurement
Per plate of 96 wells, Quick Read
Overnight Read
UM User
FOC
50.00
Charges (RM)
Non UM User
Frequent UM User
100.00
Nil
1,500.00/50 plates
needed.
Contact persons
Prerequisites
Revised Rate 27 Dec 2012 Guidelines for using the HIR Microplate Reader (F200 Pro)
The HIR Microplate Reader (F200 Pro) is a multi-user facility which provides advance
instrumentation to researchers to read the absorbance, luminescence and fluorescence at
different time frame. The measurement of absorbance, luminescence and fluorescence is fully
automated by the machine and hence it is a very easy to use machine. All consumable (96 or 384
well plate) shall be provide by user. User are responsible to make sure the machine is not
contaminated with their sample (especially bacteria sample) after usage. User are advice to
preset the temperature of the machine 15min before use.
Location
The HIR Microplate Reader (F200 Pro) is located at Central Facility 2, Level 1, HIR Building.
Operating Hours
The HIR Microplate reader (F200 Pro) is available for service from 10 am to 5 pm, working
advance. No block booking is allowed. Slots for usage are set at maximum 3 days for each
usage.
Online Booking
Export of Data
Charges
Unit of Measurement
Per plate of 96 wells, Quick Read
Overnight Read
UM User
FOC
50.00
Charges (RM)
Non UM User
Frequent UM User
100.00
Nil
1,500.00/50 plates
needed.
Contact persons
Prerequisites
Guidelines for using the HIR Microscopes (High Resolution Compound Microscope, Digital
Motorized Inverted Microscope with Fluorescence and Digital Imaging System and Stereo
microscope)
The HIR Microscopes facility is a multi-user facility which provides resarchers with various types of
microscopes for latest microscopy work. The Committee reserves the right to make any necessary changes
to the guidelines from time to time and as and when needed.
Location
The HIR Microscopes facility is at present located Microscopy Room, Level 2, HIR Building.
Operating Hours
The HIR Microscopes facility is available for use from 9 am to 5 pm, working weekdays. Weekend or
responsible officers. Advance appointment is required. Time could be booked up to 1 day in advance. Walkin usage will only be allowed provided there is no prior booking and in such instance, the user will still need
to do the booking. No block booking is allowed.
Online Booking
charge, to maximize availability for the entire UM community. Please keep your appointments, and show up
for them promptly.
User has to complete the experiments within the time slot allocated. Failing to do so, the officer has the right
to abort the experiment and vacate the machine for the next user. Any users who have booked the
(provided proper booking and all other procedures complied), also in such instances, the users who failed to
turn up on time will still be liable to pay the full amount of rental (if applicable).
User will be billed for 100% of the charges for late cancellations. Users will have to fill up a letter of
Export of Data
Charges
Rental for usage of either (i) Up-right Fluorescence, (ii) Inverted Fluorescence or (iii) Stereo microscope:
Half-day slot (either from 9.00 am – 1.00 pm or from 1.00 – 5.00 pm) is RM10.00
For frequent users: 50 hour package is available at a discounted rate of RM100.00, 100-hour package at
RM180.00 and 500-hour package at RM750.00. These discounted packages are available only to UM users
with priority to UM HIR grant holders.
For non-UM users, both three-hour and nine-to-five working hour slots are chargeable at RM100 and
RM200, respectively.
Contact persons
[email protected]), Dr Lim Phaik Em (03-79677180, [email protected]) and Dr. Ea Chee Kwee (0379675889, [email protected]).
Prerequisites
liability.
Guidelines for using the HIR Nanostring (GEN2) Facility
The HIR Nanostring (GEN2) Facility which is based on a digital molecular barcoding technology is
a multi-user facility that can deliver highly multiplexed, direct digital profiling of individual
molecules in a single reaction without amplification or bias. It application includes profiling
hundreds of gene transcripts, copy number variations, or miRNAs with high sensitivity and
precision. The NanoString nCounter gene expression system was reported to be more sensitive than
microarrays but similar to real-time PCR (Taqman and SYBRGreen).
Location
The HIR Nanostring (GEN2) Facility is at present located at the 1st Floor Centre HIR lab, HIR
Building.
Operating Hours
The HIR Nanostring (GEN2) Facility is available for service from 10 am to 5 pm, working
Strictly no block booking is allowed. The PrepStation can only work on one single catridge (~3hr)
at a single time while the Digital analyzer can scan maximum 6 catridge at any time (~6hr).
Online Booking
discretion of the officer-in-charge, to maximize availability for the entire UM community. Please
Export of Data
Data has to be saved using the original USB thumbdrive that come along with the kit (containing
the RLF file).
Charges
Currently, there will be no charge to encourage users to try and use the equipment. The Committee
Contact persons
For further information, please contact the officer in charge Dr Ea Chee Kwee (03-79675889, [email protected]), Dr. Lim Yat Yuen ([email protected])
Prerequisites
The system includes a fully automated sample Prep Station, a Digital Analyzer. Users must have
received training on this machine or demonstrate knowledge of the equipment before use. User has
to run the machine according to SOP. User will have to agree to and sign a letter of indemnity and
liability. In order to run assays using the Nanostring system, CodeSet (molecular barcodes) and all
reagents needed to perform the analysis are needed and to be bought individually. Please bring the
USB stick that comes along with the assay kit to upload the RLF file onto the Digital counter
(incase the RLF file have not been uploaded before).
time and as and when needed
Guidelines for using the Bio-Rad Next Generation Chromatography (NGC) Facility
The Bio-Rad Next Generation Chromatography (NGC) Facility is a multi-user facility which
provides instrumentation to researchers for chromatography / protein purification.
Location
The Bio-Rad Next Generation Chromatography (NGC) Facility is located at Central Facility 2.1,
Level 2, HIR building.
Operating Hours
The Bio-Rad Next Generation Chromatography (NGC) Facility is available for service from 9 am
to 5 pm, working weekdays. Advance appointments are required. Time could be booked up to two
weeks in advance. No block booking is allowed. The number of people that can be present from
each user laboratory is limited to two, except by specific pre-arrangement with the facility staff.
Online Booking
within the time slot allocated. Failing to do so, the officer has the right to abort the experiment
and vacate the machine for the next user. Cancellation of booking must be made at least two
working days before the actual equipment operation time. You will be billed for 100% of the
charges for late cancellations. Users will have to fill up a letter of intent available online.
Export of Data
Charges
hour-package is avalaible at a discounted rate of RM4500 and a 500-hour-package is available at
RM10,000. This discounted package is available only for UM researchers with priority for UM
Contact persons
[email protected]), or Dr Lau Yee Ling (03-79674749)
Prerequisites
2nd Version
Guidelines for HIR Central Facility: NucliSENS easyMAG
The NucliSENS easyMAG is a multi-user facility which provides advanced instrumentation
for total nucleic acid purification.
Location
The NucliSENS easyMAG is at present located at Central Facility 2, Level 1, HIR building.
Operating Hours
The NucliSENS easyMAG is available for service from 10 am to 5 pm, working weekdays.
Advance appointments are required. Time could be booked up to two weeks in advance. No
block booking is allowed. Slots for usage are set at 2 hours per run of 24 samples. The
Online Booking
Export of Data
Charges
The NucliSENS easyMAG operates effectively in the multiple of eight (8) samples. The
charge for every 8 samples is RM320 for UM researchers and RM800 for non-UM
researchers. For users with more than 48 samples, a 5% discount will be given. This
discounted package is available only for UM researchers with priority for UM HIR grant
holders. Lysis Buffer (#280134) and Magnetic Silica (#280133) will not be provided. All
charges are not inclusive of general reagents or consumables. The Committee reserves the
right to make any necessary adjustment to the charges when needed.
Contact persons
(03-7967 7075, [email protected]), Dr. Tee Kok-Keng ([email protected])
Prerequisites
Guidelines for using the HIR Orbitrap Fusion Mass Spectrometer
The HIR Orbitrap Fusion Mass Spectrometer which includes (a) UHPLC (b) NanoLC System facility is a
multi-user facility which provides resarchers with cutting edge on Orbitrap mass spectrometry analysis. The
Committee reserves the right to make any necessary changes to the guidelines from time to time and as
and when needed.
Location
The HIR Orbitrap Fusion Mass Spectrometer facility is at present located at Ground Floor, HIR Building.
Operating Hours
The HIR Orbitrap Fusion Mass Spectrometer facility is available for use from 9 am to 5 pm, working
weekdays. Weekend or holdiay booking requires a separate booking that user will have to inform by email
the lab officer and responsible officers. Advance appointment is required. Time could be booked up to 1
day in advance. Walk-in usage will only be allowed provided there is no prior booking and in such instance,
the user will still need to do the booking. No block booking is allowed.
Online Booking
Export of Data
Charges
For three-hour (and/or shorter time) is levied at RM200. For 1 day (nine-to-five working hour) slot and 2day slot, it is available at RM300 and RM400, respectively, for UM researchers. Each three-hour slot is
chargeable RM400. For 1 day (nine-to-five working hour) slot and 2-day slot, it is available at RM1,000 and
RM1,500, respectively, for non-UM researchers.
For frequent users, 10 days-package is available at a discounted rate of RM1,500 per package. This package
is available only for UM researchers.
Contact persons
[email protected]), Dr Loke Mun Fai ([email protected]) and Dr. Chan Kok Gan (03-79674509,
[email protected])
Prerequisites
and liability.
Guidelines for using the Hamamatsu Photon Camera
The HIR Hamamatsu Photon Camera facility is a multi-user facility which provides advanced
instrumentation to researchers for most sensitive bioimaging with ultra senstive CCD camera. The
Committee reserves the right to make any necessary changes to the guidelines from time to time
Location
The HIR Hamamatsu Photon Camera facility is at present located at Ground Floor, HIR Building.
Operating Hours
The HIR Hamamatsu Photon Camera facility is available for service from 9 am to 5 pm, working
Online Booking
Export of Data
Charges
Each three-hour (and/or shorter time) is available gratis (free-of-charge) for UM researchers. Each
three-hour slot is chargeable RM150 per slot for non-UM researchers. For nine-to-five working
hour, it is chargeable at RM50 per slot for UM researchers and RM300 per slot for non-UM
researchers.
For frequent users, 100 hour-package is available at a discounted rate of RM200 per package. This
package is available only for UM researchers with priority for UM HIR grant holders.
Contact persons
Prerequisites
Guidelines for using the Bio-Rad PROTEAN i12 IEF system
The PROTEAN i12 IEF system is designed for isoelectric focusing (IEF) proteins in immobilized pH
gradient (IPG) strips for the first dimension of two-dimensional (2-D) electrophoresis. The PROTEAN
i12 IEF cell can run 1–12 IPG strips in 7, 11, 13, 17, 18, or 24 cm focusing trays. Each channel in the
isoelectric focusing tray is independently powered, enabling precise control over each IPG strip. This
feature makes it possible to run different sample types, different pH gradients, and multiple
protocols simultaneously.
Location
The PROTEAN i12 IEF system is located at Central Facility 2.1, Level 2, HIR building.
Operating Hours
The PROTEAN i12 IEF system is available for service from 9 am to 5 pm, working weekdays. Advance
appointments are required. Time could be booked up to two weeks in advance. No block booking is
allowed. The number of people that can be present from each user laboratory is limited to two,
except by specific pre-arrangement with the facility staff.
Online Booking
your appointments, and show up for them promptly. User has to complete the experiments within
the time slot allocated. Failing to do so, the officer has the right to abort the experiment and vacate
the machine for the next user. Cancellation of booking must be made at least two working days
before the actual equipment operation time. You will be billed for 100% of the charges for late
Export of Data
Charges
Each day is chargeable at RM15 for UM researchers and RM30 for non-UM researchers. For frequent
users, a one-month-package is avalaible at a discounted rate of RM270. This discounted package is
available only for UM researchers with priority for UM HIR grant holders. All charges are not
inclusive of reagents and consumables. The Committee reserves the right to make any necessary
adjustment to the charges when needed.
Contact persons
[email protected]) or Dr. Lau Yee Ling (03-79674749, [email protected]).
Prerequisites
letter of indemnity and li
Guidelines for using the Bio-Rad ProteOn XPR36 system Facility
The Bio-Rad ProteOn XPR36 system Facility is a multi-user facility which provides instrumentation to researchers
for protein-interaction studies.
when needed.
Location
The Bio-Rad ProteOn XPR36 system Facility is located at Central Facility 2.1, Level 2, HIR building.
Operating Hours
The Bio-Rad ProteOn XPR36 system Facility is available for service from 9 am to 5 pm, working weekdays. Advance
appointments are required. Time could be booked up to two weeks in advance. No block booking is allowed. The
number of people that can be present from each user laboratory is limited to two, except by specific prearrangement with the facility staff.
Online Booking
availability for the entire UM community. Please keep your appointments, and show up for them promptly. User
has to complete the experiments within the time slot allocated. Failing to do so, the officer has the right to abort
the experiment and vacate the machine for the next user. Cancellation of booking must be made at least two
working days before the actual equipment operation time. You will be billed for 100% of the charges for late
Export of Data
Charges
UM user:
RM 50/day
RM1000 for maintenance chip
RM1000 for cleaning chip
Non-UM user:
RM 200/day
RM3000 for maintenance chip
RM2000 for cleaning chip
Note:
-Maintenance chip will be applicable for long-term shutdown in HIR’s setting (more than a week of without usage).
So once a user has done the experiment and will not come back to use the instrument for more than 1 week, the
maintenance chip should be inserted into the system.
-Cleaning chip will be used by end user upon completion of their experiment with the same protein. This is for
cleaning of the microfluidics channel to prevent risk of cross-contamination. So, each time a user finishes their
experiment, they will use 1 cleaning chip.
All charges are not inclusive of reagents and consumables. The Committee reserves the right to make any
necessary adjustment to the charges when needed.
Contact persons
For further information, please contact the officer in charge, Ms Lim Moo Eng (03-79677075, [email protected])
or Dr. Lau Yee Ling (03-79674749) [email protected]
Prerequisites
2nd Version
Guidelines for using the HIR Pulse Field Gel Electrophoresis (PFGE)
The HIR PFGE resolves DNA by alternating electrical field between distinc pairs of electrodes.
PFGE is good to resolve large DNA fragment of >10Mb, and chracterisation of genome size.
HIR discourage staining of PFGE gels with ethidium bromide which is carcinogenic. HIR also
provide high resolution gel imaging system for image capture of PFGE. The Committee reserves
the right to make any necessary changes to the guidelines from time to time and as and when
needed.
Location
The HIR PFGE is at present located at the HIR building,1st Floor, Central Lab.
Operating Hours
The HIR PFGE is available for service from 10 am to 5 pm, working weekdays. Advance
booking is allowed.
Online Booking
least one working day before the actual equipment operation time. You will be billed for 50% of
the charges for late cancellations. Users will have to fill up a letter of intent available online.
Export of Data
Charges
For half-day usage, it will be free-of-charge for UM researchers and RM400 for non-UM
researchers. For one-day usage, it will be free-of-charge for UM researchers, and RM800 for
non UM researchers. The Committee reserves the right to make any necessary adjustment to the
Contact persons
7075, [email protected]), Responsible officers are Dr. Chan Kok Gan (03-79674509,
[email protected]) and Dr Ea Chee Kwee ([email protected]).
Prerequisites
Guidelines for using the ABI Quantstudio 12K Real Time PCR Facility
The HIR ABI Quantstudio 12K Real Time Facility is a multi-user facility which provides
advanced instrumentation to researchers for high throughput qPCR etc.
Location
The HIR ABI Real Time Facility is at present located at Central Facility 2, Level 1, HIR
building.
Operating Hours
The HIR ABI Real Time Facility is available for service from 9 am to 5 pm, working
Online Booking
Export of Data
Charges
For frequent users, a 100 hour-package is available at a discounted rate of RM2500 per
package, a 200 hour-package is avalaible at a discounted rate of RM4000 and a 500-hour
package is available at RM9000. This discounted package is available only for UM researchers
with priority for UM HIR grant holders. All charges are not inclusive of reagents and
consumables. The Committee reserves the right to make any necessary adjustment to the
Contact persons
[email protected]), Dr. Ng Ching Ching (03-79675872).
Prerequisites
Revised Rate 27Mar 2013 Guidelines for HIR Central Facility: Qubit 2.0 Fluorometer
The Qubit 2.0 Fluorometer is a multi-user facility which provides advanced instrumentation
for fluorometric quantitation of biomolecules such as DNA, RNA and protein.
Location
The Qubit 2.0 Fluorometer is at present located at the Central Facility 2, Level 1, HIR
Building.
Operating Hours
The Qubit 2.0 Fluorometer is available for service from 10 am to 5 pm, working weekdays.
block booking is allowed. Slots for usage are set at 10 minutes (DNA, RNA) or 25 minutes
(protein) per sample. The number of people that can be present from each user laboratory is
limited to two, except by specific pre-arrangement with the facility staff.
Online Booking
Export of Data
Charges
Each sample will be FOC for UM researchers and RM25 for non-UM researchers. All
charges are not inclusive of reagents or consumables. The Committee reserves the right to
make any necessary adjustment to the charges when needed.
Contact persons
(03-7967 7075, [email protected]), Dr. Tee Kok-Keng ([email protected])
Prerequisites
Guidelines for using the HIR Eyela Rotary Evaporator Facility
The HIR Eyela Rotary Evaporator Facility is an apparatus developed as advanced
instrumentation to researchers for rapid removal of excess solvent under reduced pressure in
the microscale laboratory.
Location
The HIR Eyela Rotary Evaporator Facility is at present located at the Fume Hood Room,
Level 1, HIR Building.
Operating Hours
The HIR Eyela Rotary Evaporator Facility is available for service from 10 am to 5 pm,
working weekdays. Advance appointments are required. Time could be booked up to two
weeks in advance. No block booking is allowed. The number of people that can be present
from each user laboratory is limited to two, except by specific pre-arrangement with the
facility staff.
Online Booking
utilize the machine. The slots are scheduled on a first-booked-first-served basis. Confirmation
is done at the discretion of the officer in charge, to maximize availability for the entire UM
booking must be made at least two working days before the actual equipment operation
time.You will be billed for 50% of the charges for late cancellations. Users will have to fill
up a letter of intent available online.
Export of Data
None
Charges
Each one hour is chargeable at RM30 for UM researchers and RM100 for non-UM
researchers. For frequent users, 100 hour-package is available at a discounted rate of
RM2500 per package and 200 hour-package is avalaible at a discounted rate of RM4000.
This package is available only for UM researchers with priority for UM HIR grant holders.
needed.
Contact persons
For further information, please contact the officer in charge Ms Lim Moo Eng (03-7967
7075, [email protected]), Dr. Chan Kok Gan (03-79675162, [email protected])
Prerequisites
Guidelines for using the Bio-Rad SDS Gel Electrophoresis System
The SDS PAGE Gel Electrophoresis system is designed for proteins separating using SDS
PAGE.
Location
The SDS PAGE Gel Electrophoresis system is located at Central Facility 2.1, Level 2, HIR
building.
Operating Hours
The SDS PAGE Gel Electrophoresis system is available for service from 9 am to 5 pm,
working weekdays. Advance appointments are required. Time could be booked up to two
weeks in advance. No block booking is allowed. The number of people that can be present
from each user laboratory is limited to two, except by specific pre-arrangement with the
facility staff.
Online Booking
Export of Data
Charges
Each day is chargeable at RM10 for UM researchers and RM15 for non-UM researchers. For
frequent users, a one-month-package is avalaible at a discounted rate of RM200. This
holders. All charges are not inclusive of reagents and consumables. The Committee reserves
the right to make any necessary adjustment to the charges when needed.
Contact persons
For further information, please contact the officer in charge, Ms Lim Moo Eng
(03-79677075, [email protected]) or Dr. Lau Yee Ling (03-79674749,
[email protected]).
Prerequisites
to and sign a letters of indemnity and liability
Guidelines for using the HIR Tabletop Scanning Electron Microscopes (Tabletop SEM)
The HIR Tabletop SEM facility is a multi-user facility which provides resarchers with cutting edge Tabletop
SEM for latest electron microscopy work. The Committee reserves the right to make any necessary changes
to the guidelines from time to time and as and when needed.
Location
The HIR Microscopes facility is at present located at SEM Room, Level 2, HIR Building.
Operating Hours
The HIR Microscopes facility is available for use from 9 am to 5 pm, working weekdays. Weekend or holdiay
booking requires a separate booking that user will have to inform by email the lab officer and responsible
officers. Advance appointment is required. Time could be booked up to 1 day in advance. Walk-in usage
will only be allowed provided there is no prior booking and in such instance, the user will still need to do
Online Booking
Export of Data
Charges
For three-hour (and/or shorter time) is RM100 and for nine-to-five working hour slots are available at
RM200 per day for UM researchers. Each three-hour slot is chargeable RM200 and RM400, respectively,
per slot, for non-UM researchers.
Contact persons
[email protected]), Dr Lim Phaik Em (03-79677180, [email protected]) Dr. Chan Kok Gan (0379674509, [email protected])
Prerequisites
and liability.
Guidelines for using the Bio-Rad Mini Transblot Cell System
The Transblot system is designed for Western blotting to transfer proteins from SDS PAGE
to membrane.
Location
The Transblot system system is located at Central Facility 2.1, Level 2, HIR building.
Operating Hours
The Transblot system system is available for service from 9 am to 5 pm, working weekdays.
block booking is allowed. The number of people that can be present from each user
laboratory is limited to two, except by specific pre-arrangement with the facility staff.
Online Booking
Export of Data
Charges
Each day is chargeable at RM10 for UM researchers and RM15 for non-UM researchers. For
frequent users, a one-month-package is avalaible at a discounted rate of RM200. This
Contact persons
For further information, please contact the officer in charge, Ms Lim Moo Eng (0379677075, [email protected]) or Dr. Lau Yee Ling (03-79674749,
[email protected]).
Prerequisites
to and sign letters of indemnity and liability.
Guidelines for HIR Central Facility: Typhoon FLA 9500
Typhoon FLA 9500 is a variable mode laser scanner with modular access to the optical
components, providing both versatile and flexible imaging for precise quantitation of
proteins, nucleic acids, and other biomolecules.
Location
The Typhoon FLA 9500 is at present located at the HIR building central facility room2.
Operating Hours
The Typhoon FLA 9500 is available for service from 10 am to 5 pm, working weekdays.
Advance appointments are required. Time could be booked up to two weeks in advance.
Online Booking
operation time. Users will have to fill up a letter of intent available online.
Export of Data
Charges
The Typhoon FLA 9500 is FOC for UM-researcher and RM50 per scan for non-UM
researchers. The Committee reserves the right to make any necessary adjustment to the
Contact persons
(03-7967 7075, [email protected]), Dr. Ea Chee Kwee ([email protected])
Prerequisites
Guidelines for using the HIR Agilent 1290 Ultra High Performace Liquid Chromatograph
(UHPLC)
The HIR Agilent 1290 UHPLC Facility is a multi-user facility which provides advanced
instrumentation to researchers for qualitative and quantitative analysis of various types of polar and
non-polar molecules of low or high molecular weight samples.
User must provide reference of journals, publication or application notes to confirm that the samples
must be amenable to LC techniques and reference to HPLC methods such as suitable mobile phase,
gradient composition, types of LC columns and detectors are available. Samples must go through
proper sample preparation or clean-up procedure prior to LC injection.
Location
The HIR Agilent 1290 UHPLC Facility is located at Bilik Khas 3, Ground Floor, HIR building.
Operating Hours
The HIR Agilent 1290 UHPLC Facility is available for service from 10 am to 5 pm, working
No block booking is allowed. Slots for usage are set at three-hours each i.e. 10 am – 1 pm and 2 pm
– 5 pm. The number of people that can be present from each user laboratory is limited to two, except
by specific pre-arrangement with the facility staff.
Online Booking
Export of Data
Charges
Each three-hour slot is chargeable at RM15 per sample for UM researchers and RM50 per slot for
non-UM researchers. For frequent users, 50 slot-package is available at a discounted rate of RM600
per package and 100 slot-package is available at a discounted rate of RM 1000. This package is
available only for UM researchers with priority for UM HIR grant holders. The Committee reserves
Contact persons
Prerequisites
before use. User has to run the machine according to SOP. User will have to agree to and sign a letter
of indemnity and liability.
Guidelines for using the Beckman Coulter Optima XPN100 Ultracentrifuge Facility
The Beckman Coulter Optima XPN100 Ultracentrifuge Facility is a multi-user facility which
provides instrumentation to researchers for ultracentrifugation: purify and/or separation of
viruses, subcellular organelles, macromolecules etc (SW 41 Ti, Type 70 Ti, and Type 100 Ti
rotors are available, which rated for 41 000 rpm, 70 000 rpm, and 100 000 rpm, respectively).
Location
The Beckman Coulter Optima XPN100 Ultracentrifuge Facility is at present located at Bilik
Khas 2, HIR building.
Operating Hours
The Beckman Coulter Optima XPN100 Ultracentrifuge Facility is available for service from 9
am to 5 pm, working weekdays. Advance appointments are required. Time could be booked up
to two weeks in advance. No block booking is allowed. The number of people that can be
present from each user laboratory is limited to two, except by specific pre-arrangement with the
facility staff.
Online Booking
your schedule. The slots are scheduled on a first-booked-first-served basis. Confirmation is done
Export of Data
Not applicable.
Charges
hour-package is avalaible at a discounted rate of RM1800 and a 500-hour-package is available at
RM3000. This discounted package is available only for UM researchers with priority for UM
Contact persons
[email protected]), Dr. Ng Ching Ching (03-79675872).
Prerequisites
Guidelines for using the Vacuum Concentrator
The HIR Vacuum Concentrator facility is a multi-user facility which provides resarchers with
Vacuum Concentrator for rapid drying of biomolecules. The Committee reserves the right to make
any necessary changes to the guidelines from time to time and as and when needed. Users must
ensure the solvent used is compatible to the Vacuum Concentrator.
Location
The HIR Vacuum Concentrator facility is at present located at Microbiom 3, Level 1, HIR
Building.
Operating Hours
The HIR Vacuum Concentrator facility is available for service from 9 am to 5 pm, working
Online Booking
Export of Data
Data has to be saved using CD only (if applicable).
Charges
Contact persons
Prerequisites
Guidelines for using the VITEK® 2 System
The Biomérieux VITEK® 2 system is a multi-user facility which provides instrumentation to
researchers for microbial identification and antibiotic susceptibility testing.
Location
The Biomérieux VITEK® 2 system is located at Central Facility 2, Level 1, HIR building.
Operating Hours
The Biomérieux VITEK® 2 system is available for service from 9 am to 5 pm, working
Online Booking
Export of Data
Charges
Each day (24 hours) is chargeable at RM100 for UM researchers and RM200 for non-UM
researchers. For frequent users, a 10 day-package is avalaible at a discounted rate of RM800.
This discounted package is available only for UM researchers with priority for UM HIR grant
Contact persons
[email protected]), Dr Chai Lay Ching ([email protected]) and Prof Ngeow Yun Fong
([email protected])
Prerequisites

Guidelines for Equipment Usage

Transcription

Similar documents

Working scientifically outside the classroom

Assessment in the new Primary Science curriculum...a world without

Leading effective professional development in science

Name: RAYMOND CRUZ

Please use one form per client, send the filled booking form back to

ONLINE TRAVEL AGENT BOOKING NOW LIVE!

Booking Form_Corected

How to book a XCN Surface + CHPP Induction

NYE 14 -15 BOOKING CONFIRMATION FORM

Room Bookings Chart - Clare Conferencing