Program - The 3rd Prato conference on Pore Forming

PROGRAM Monday 11th May 2015 Registration 3:00pm -­‐ 5:30pm Welcome Reception 5:30pm -­‐ 7:00pm Tuesday 12th May 2015 Welcome Address 9:00am -­‐ 9:30am Chairs: Michelle Dunstone & Ilia Voskoboinik Session 1: Plenary Session 9:30am -­‐ 10:30am Chairs: Michelle Dunstone 9:30 AM Johannes van Strijp Immune evasion by staphylococcal toxins abs# 1 Morning Tea 10:30am -­‐ 11:00am Session 2: Applications of electrophysiology of pore forming proteins 11:00am -­‐ 12:30pm Chair: James Whisstock Session sponsored by: 11:00 AM Mathias Winterhalter Quantification of antibiotic uptake across bacterial cell walls abs# 2 11:30 AM Jan Behrends High-­‐resolution electrophysiological analysis of pore-­‐forming proteins using parallel lipid bilayer microarrays abs# 3 11:50 AM Roland Benz Structure and function of cell wall channels of mycolic acid containing members of the actinomycetes abs# 4 12:10 PM Joseph Robertson Pore-­‐forming proteins: Simultaneous structure-­‐function methods & potential applications abs# 5 Lunch 12:30pm -­‐ 1:30pm Session 3: New approaches to study pore forming proteins 1:30pm -­‐ 3:00pm Chair: Ian Smith 1:30 PM Doryen Bubeck Structural analysis of the complement membrane attack complex by Cryo-­‐EM and single particle analysis abs# 6 2:00 PM Bryan Krantz Allosteric and stereoselective clamps govern protein translocation through the anthrax toxin protective antigen channel abs# 7 2:20 PM Michael Senior Imaging the assembly of perfringolysin O abs# 8 2:40 PM Bart Hoogenboom Stepwise visualization of membrane pore formation by suilysin, a bacterial cholesterol-­‐dependent cytolysin abs# 9 Afternoon Tea & Poster Session 3:00pm -­‐ 5:00pm, Abstract numbers 34 – 51, includes wine and cheese service Wednesday 13th May 2015 Session 4: Structural and mechanistic insights into pore forming proteins 9:00am -­‐ 10:30am Chair: Doryen Bubeck 9:00 AM Hong Zhou Atomic model of the anthrax toxin pore by cryo electron microscopy abs# 10 9:30 AM Cyril Reboul A new model for pore formation by cholesterol-­‐dependent cytolysins abs# 11 9:50 AM Natalya Dudkina Stepwise visualisation of membrane pore formation by suilysin, a cholesterol dependent cytolysin abs# 12 10:10 AM Andrew Ellisdon Stonefish toxin defines an extensive new branch of the perforin-­‐like protein superfamily abs# 13 Morning Tea 10:30am -­‐ 11:00am Session 5: Macromolecular structure and assembly 11:00am -­‐ 12:40pm Chair: Hong Zhou 11:00 AM Stefan Raunser How to kill a mocking bug -­‐ Structural Insights into Tc toxin complex action abs# 14 11:30 AM Michael Parker Cholesterol-­‐dependent cytolysins: From water-­‐soluble state to membrane pore abs# 15 11:50 AM Bradley Spicer Defining a site important for zinc inducible polyC9 formation abs# 16 12:10 PM Natalya Lukoyanova The trajectory of β-­‐sheet opening during MACPF pore formation abs# 17 Lunch 12:40pm -­‐ 1:40pm Session 6: Hot topics and new frontiers 1:40pm -­‐ 3:00pm Chair: Bryan Krantz 1:30 PM Paul Conroy 1:50 PM Amelia Brennan 2+
A novel role for Ca in cytotoxic lymphocyte perforin proteostasis abs# 19 2:10 PM M.A. Henstridge The Membrane Attack Complex/ Perforin-­‐like protein Torso-­‐like controls growth factor secretion to pattern the early Drosophila embryo abs# 20 2+
Structural basis for Ca -­‐mediated interaction of the perforin C2 domain abs# 18 2:30 PM Nadja Hellmann Dual effect of P2X-­‐inhibitors on lysis induced by a-­‐toxin from S.aureus abs# 21 Afternoon Tea & Poster Session 3:00pm -­‐ 5:00pm, Abstract numbers 34 -­‐ 51, includes wine and cheese service Thursday 14th May 2015 Session 7: Pore forming proteins in infection, immunity and venom 9:00am -­‐ 10:30am Chair: Andrew Ellisdon 9:00 AM Victor Torres Dissecting the mechanism by which Staphylococcus aureus leukocidins target and kill host leukocytes abs# 22 9:30 AM Emmanuel Jover The staphylococcal Panton and Valentine Leukocidin and γ-­‐haemolysin HlgC/HlgB share C5aR as a receptor, but operate diverse intracellular activities in human polymorphonuclear neutrophils abs# 23 9:50 AM Michael Jennings The cholesterol-­‐dependent cytolysis, pneumolysin and streptolysin O, require binding to red blood cell glycans for hemolytic activity abs# 24 10:10 AM Gintaras Valincius Activity of pore-­‐forming cholesterol dependent cytolysins assessed by dielectric damage of tethered bilayer membranes abs# 25 Morning Tea 10:30am -­‐ 11:00am Session 8: Clinical immunology and microbiology 11:00am -­‐ 12:30pm Chair: Salvatore Valitutti 11:00 AM Juliane Bubeck Wardenburg Staphylococcus aureus alpha-­‐toxin: Complexities of the host-­‐pathogen interaction abs# 26 11:30 AM Matthias Husmann Nutrient sensor GCN2 confers tolerance to bacterial pore forming toxins abs# 27 11:50 AM Tamara Reyes-­‐Robles A dominant-­‐negative approach to combat Staphylococcus aureus pathogenesis abs# 28 12:10 PM Joseph Trapani Failed CTL/NK cell killing and cytokine hyper-­‐secretion are directly linked through prolonged synapse time abs# 29 Lunch 12:30pm -­‐ 1:30pm Session 9: Cell biology and pore forming proteins 1:30pm -­‐ 3:00pm Chair: Ilia Voskoboinik 1:30 PM Matthew Pipkin Unraveling how chromatin structure regulates cytotoxic T lymphocyte differentiation abs# 30 2:00 PM Stephanie Seveau Plasma membrane attack by cholesterol-­‐dependent cytolysins stimulates the internalization of the CDC-­‐producing pathogen abs# 31 2:20 PM Imran House Heterozygosity for the common perforin mutation, A91V, impairs the cytotoxicity of primary natural killer cells from healthy individuals abs# 32 2:40 PM Jonathan Richardson Dual roles for a novel pore-­‐forming peptide toxin in the human fungal pathogen Candida albicans abs# 33 Afternoon Tea & Poster Prizes 3:00pm -­‐ 4:00pm Poster prizes awarded, includes wine and cheese service Conference Dinner 6:30pm -­‐ 10:30pm Dinner location: Reffetorio di San Niccolo Piazza Niccolo Cardinale, 6, Prato, Italy Approximately 800 meter walk (10 mins) from Monash University. The dinner will commence with a short tour of the Reffetorio followed by welcome drinks and canapés, a three course meal and drinks.