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12123 MolDim Supplement 24pp UPDATED_Layout 1 8/4/15 12:16 PM Page 1
MOLECULAR
DIMENSIONS
Providing you with the
latest innovations in
protein crystallography
MORE
INTELLIGENT
SOLUTIONS
Intelligent solutions for
protein crystal growth
01
Crystal Growth Screens
Soluble Proteins
05
CONTENTS
Welcome to More Intelligent Solutions an
update to our printed catalogue. Since last
published we have added many new
products as a result of our own
developments and our continued
collaborations with leading researchers and
developers. Progress continues to be made
in the field of structural biology with new,
highly successful screens for both soluble
and membrane proteins. We have introduced
a new version of the unique Calixar additives
that facilitate crystal contact formation, and
a new kit for crystal cryoprotection and
soaking of hydrophobic ligands.
Membrane Proteins
07
Dialysis & Ultrafiltration
Protein Production
10
Rigaku Crystallization Plates
Sitting Drop Plates
Dialysis & Ultrafiltration
In this catalogue supplement you will find an
exciting new range of loops with the latest
technology for improved data collection,
Uni-Pucks and a new range of dewars. We
are particularly excited to offer a new range
of modern products for dialysis and
ultrafiltration. And for viewing crystal growth
trays you can now find a choice of
microscopes and the UVEX UV fluorescence
imaging systems.
12
High-Throughput Plates
LCP Plates
17
Crystallography
Instrumentation
13
Cryocrystallography
18
Microscopes
20
UVEX Imaging Systems
CRYSTAL
GROWTH
SCREENS
MOLECULAR DIMENSIONS
SUPPLIES A WIDE RANGE OF
CRYSTAL GROWTH SCREENS FROM
CLASSIC SPARSE MATRICES TO
MODERN HIGH EFFICIENCY
PRODUCTS EXTENSIVELY
COVERING CRYSTALLIZATION
SPACE FOR GLOBULAR PROTEINS,
MEMBRANE PROTEINS AND
NUCLEIC ACIDS.
02 Crystal Growth Screens
02 Morpheus II Screen
02 Morpheus Additive Screen
03 SG1™ Screen
03 Wizard Classics
04 Wizard Cryo™
04 Wizard JSCG+™
04 Wizard Peg Ion™
04 Wizard Precipitant Synergy™
05 MemMeso™
06 Wizard™ Cubic LCP Kit
06 Wizard Time Screen™
07 Calixar™ V2.0
North & South America orders: +1 877 479 4339
SOLUBLE PROTEINS
A crystal growth screening strategy requires
a comprehensive coverage of crystallization
space with both a proven set of conditions as
well as a systematic approach to provide
useful information for further
experimentation. In addition deeper
interrogation of crystal growth potential
should be available with novel precipitants
and approaches.
Molecular Dimensions provides a wide choice
of screens including high efficiency, low
redundancy sparse matrices such as JCSG
plus™ and the more recent shotgun SG1™,
systematic approaches with products like
PACT premier™, novel precipitant products
with MIDAS™ and PGA™, and new directions
as with Morpheus®.
For a full list of Molecular Dimensions’ crystal
growth screens including products for
protein complexes and nucleic acids please
refer to our main catalogue or our web site
at www.moleculardimensions.com.
email: [email protected]
visit: www.moleculardimensions.com
1
SOLUBLE PROTEINS
SOLUBLE PROTEINS
MORPHEUS® II SCREEN
MORPHEUS® II IS A FOLLOW-UP TO THE BEST-SELLING
96-CONDITION SCREEN, MORPHEUS. OPTIMISED TO YIELD
CRYSTALS NOT OBSERVED IN TRADITIONAL SCREENS,
IT ALSO CONTAINS HEAVY ATOMS FOR EXPERIMENTAL
PHASING AND POLYOLS FOR CRYOPROTECTION.
Morpheus® II integrates reagents that are not seen in other screens,
especially less common additives. As a consequence, the screen
should have an impact not only on crystallisation but also on the
overall structure determination process.
It follows the general design principles of the original Morpheus
screen, but less typical additives have been included, such as heavy
metals, NDSBs, polyamines, amino acids and monosaccharides. In
addition, various polyols have been added for cryoprotection along
with innovative buffer systems.
MORPHEUS®
ADDITIVE SCREEN
USE THE POWER OF MORPHEUS TO OPTIMIZE YOUR
CRYSTALS. MORPHEUS ADDITIVE SCREEN IS A NEW
96-CONDITION ADDITIVE SCREEN WHICH CONTAINS A
UNIQUE RANGE OF LIGANDS, HEAVY ATOMS AND POLYOLS.
Morpheus® Additive Screen uses reagents that are not seen in
other screens, especially less common additives. Some additives
such as metal divalent cations can induce new crystal contacts.
Heavy atoms were integrated to help with ab initio structure
determination. Most of the other additives are included to
enhance protein stability and solubility (carboxylic acids,
polyamines and monosaccharides).
Some additives such as metal divalent cations can induce new
crystal contacts. Heavy atoms were integrated to help with ab initio
structure determination.
Most of the other additives are meant to enhance protein stability
and solubility (carboxylic acids, polyamines and monosaccharides).
Features of Morpheus® II
Features of Morpheus Additive Screen:
n Targeted incorporation of 35 low-molecular weight PDB ligands into
96 conditions.
n Inclusion of non-detergent sulfobetaines (NDSBs), polyamines,
amino acids and monosaccharides to modify the solubility and
stability of many proteins.
n Incorporation of heavy atoms as additives for experimental phasing.
n Also ideal for membrane proteins as contains PEGS and low salt.
n The inclusion of Non-detergent sulfobetaines (NDSBs), polyamines,
amino acids and monosaccharides to enhance solubility and stability
of many proteins for crystallization.
n Contains heavy atoms which can be used for experimental phasing.
n Can also be used in protein stability screen assays e.g. thermal shift
assays.
n New polyols included for cryoprotection of conditions.
Crystals of catalase (left) and of an actin homologue (right) grown in Morpheus® II
(Courtesy of Fabrice Gorrec, MRC)
ORDER INFORMATION
ORDER INFORMATION
Description
Pack
Morpheus® II
96 x 10 mL MD1-91
Morpheus® II HT-96
96 x 1 mL
MD1-92
100 mL
MDSR-91-tube number
Morpheus II HT-96 single reagents 100 mL
MDSR-92-well number
®
Morpheus II single reagents
®
®
The Morpheus Additive Kit
Code
Description
Pack
The Morpheus® Additive Screen
96 x 100 µL
Code
MD1-93
References
Gorrec, F (2015). The Morpheus II protein crystallization screen, manuscript submitted.
Gorrec, F (2013). The current approach to initial crystallization screening of proteins is under-sampled J Appl
Cryst 46, 795-797.
96 x 100 µLMD1-93
References
Gorrec, F (2015), The Morpheus II protein crystallization screen, manuscript submitted.
Gorrec, F (2013), The current approach to initial crystallization screening of proteins is under-sampled J Appl
Cryst 46, 795-797.
2
Crystals of Polymerase complex (80 kDa)
courtesy of Fabrice Gorrec, MRC
Morpheus II and Morpheus® Additive Screen has been designed and developed by Fabrice GORREC, in
collaboration with the scientists at the Medical Research Council Laboratory of Molecular Biology (LMB) at
Cambridge and is manufactured exclusively under license by Molecular Dimensions Limited.
Europe and rest of world orders: +44 (0)1638 561 051 email: [email protected]
SOLUBLE PROTEINS
SG1™ SCREEN
WIZARD CLASSICS
SG1* (SHOTGUN SCREEN) REPRESENTS THE MOST
SUCCESSFUL, NON-REDUNDANT FREQUENTLY
REPORTED CRYSTALLIZATION CONDITIONS FROM
ALL THE EARLY COMMERCIALLY AVAILABLE
CRYSTALLIZATION SCREENS.
THE WIZARD™: CLASSIC LINE OF RANDOM SPARSE
MATRIX SCREENS IS DESIGNED TO INCREASE
YOUR PROBABILITY OF PRODUCING CRYSTALS
DURING THE COARSE SCREENING PHASE WHEN
CRYSTALLIZING BIOLOGICAL MACROMOLECULES
(PROTEINS, NUCLEIC ACIDS, PEPTIDES, AND
COMBINATIONS THEREOF).
The term "shotgun screening" was coined early in the Structural
Genomics era and refers to the process of setting up
experiments using pre-mixed cocktails until a crystal of
sufficient quality is obtained.
The Wizard Classic reagents are proven to be a highly effective
starting point in the screening of biological macromolecules. The
Wizard Classic formulations include a large range of crystallants,
buffers, and salts covering a broad range of crystallization space
at pH levels from pH 4.5 to pH 10.5.
The best place to start screening is within the context of
previously successful crystallization space. "Although only 14%
of successful crystallization conditions from the PDB are
identical to a commercial condition, almost 40% of the PDB
conditions can be obtained by trivial optimization of a
commercial cocktail.” (Fazio et al) So this is a reasonable place
to commence screening.
SOLUBLE PROTEINS
Why buy MCSG, PEG/Ion, INDEX, Crystal Screens I & II, JCSG core
1, and Classics I & II when you can buy one simple screen
containing the top hits from all of these.
Features of SG1 (ShotGun Screen):
n 96 of the most successful conditions from all the early commercially
available screens.
n Provides a great start for easy optimization.
n Save money and time.
ORDER INFORMATION
Images of HSP90 (N-term domain) crystallized in C3 for MecRx from SG1 (ShotGun)
screen (courtesy of J.Newman, CSIRO)
ORDER INFORMATION
Description
Pack
Code
Wizard Classic 1
48 x 10 mL
MD15-W1-T
Wizard Classic 1 & 2 HT-96
96 x 1.7 mL
MD15-W12-B
Wizard Classic 2
48 x 10 mL
MD15-W2-T
Wizard Classic 1 & 2
10 mL
MD15-W12-T
Description
Pack
Code
Wizard Classic 3
48 x 10 mL
MD15-W3-T
SG1 Screen
96 x 10 mL
MD1-88
Wizard Classic 3 & 4 HT-96
96 x 1.7 mL
MD15-W34-B
SG1 Screen HT-96
96 x 1.1 mL
MD1-89
Wizard Classic 4
48 x 10 mL
MD15-W4-T
SG1 Screen FX
96 x 100 µL
MD1-89-FX
Wizard Classic 3 & 4
96 x 10 mL
MD15-W34-T
SG1 ECO Screen
96 x 10 mL
MD1-88-ECO
Emerald Screen Single Reagent
100 mL
EBSR-100-
SG1 HT-96 ECO
96 x 1.1 mL
MD1-89-ECO
250 mL
EBSR-250-
References
Fazio VJ, Peat TS & Newman J (2014). A drunken search in Crystallization Space. Acta Cryst. F70:1303-1311
Wizard screens are manufactured by Rigaku Reagents
*Developed by Janet Newman (CSIRO) and manufactured under licence by Molecular Dimensions Ltd.
North & South America orders: +1 877 479 4339 email: [email protected]
3
SOLUBLE PROTEINS
SOLUBLE PROTEINS
WIZARD CRYO™
WIZARD JCSG+™
THE WIZARD CRYO LINE OF RANDOM SPARSE MATRIX
SCREENS IS DESIGNED FOR SCIENTISTS WHO WANT
TO AVOID THE ADDITIONAL STEP OF OPTIMIZING A
CRYOPROTECTANT CONDITION. EVERY WIZARD
CRYO FORMULATION FLASH-FREEZES TO A CLEAR,
AMORPHOUS GLASS IN LIQUID NITROGEN OR IN A
CRYOSTREAM AT 100K.
THE WIZARD JCSG+™ 96 WELL BLOCK PLATE SPARSE
MATRIX PROTEIN CRYSTALLIZATION SCREEN IS DESIGNED
FOR COARSE SCREENING OF INITIAL CRYSTALLIZATION AND
SOLUBILITY CONDITIONS FOR BIOLOGICAL
MACROMOLECULES.
Crystals can be frozen directly
from their growth drops, avoiding
the additional step of preequilibration with an artificial
cryo-condition that can damage
the crystal. Eleven different
cryocrystallants and sparing use
of glycerol ensures a broad
sampling of possible cryo
conditions.
Researchers from the Joint
Center for Structural Genomics
(JCSG) formulated this screen
with the goal of maximizing the
coverage of the crystallization
parameter space (buffers, salts,
precipitants, and pH) and
eliminating redundancy. The
buffers give final solution pHs
between 4.2 and 10.5.
Precipitants include volatile
reagents such as ethanol, high
molecular weight polymers such as
PEG-8000, and salts such as
ammonium sulfate.
ORDER INFORMATION
Description
Pack
Code
Wizard Cryo 1
48 x 10 mL
MD15-C1-T
Wizard Cryo 2
48 x 10 mL
MD15-C2-T
Wizard Cryo 1 & 2
96 x 10 mL
MD15-C12-T
Description
Pack
Code
Wizard Cryo 1 & 2 HT-96
96 x 1.7 mL
MD15-C12-B
Wizard: JCSG+ Screen HT-96
96 x 1.7 mL
MD15-JCSGP-B
100 mL
EBSR-100-
100 mL
EBSR-100-
250 mL
EBSR-250-
250 mL
EBSR-250-
ORDER INFORMATION
WIZARD PRECIPITANT
SYNERGY™
WIZARD PEG ION™
DISCOVER MORE PROTEIN CRYSTALLIZATION HITS WITH
THIS UNIQUE SCREEN BY EMPLOYING SYNERGISTIC
EFFECTS OF MECHANISTICALLY DISTINCT PRECIPITATING
AGENTS (SALTS, ORGANIC SOLVENTS, POLYETHYLENE
GLYCOLS, ADDITIVES, PH).
THE PEG ION SERIES MATRIX COMBINES THE
CRYSTALLIZING POWER OF POLYETHYLENE GLYCOLS (PEG
1K AND PEG 4K) WITH SALTS THAT ARE COMMONLY FOUND
IN CRYSTALLIZATION CONDITIONS.
The Wizard Precipitant Synergy crystallization screen consists of 192
formulations, 29 to be used for initial protein crystallization screening
and crystallization optimization. The application of this screen is
advised in particular for protein targets that have failed to yield hits
with traditional random sparse matrix crystallization approaches.
Use PEG Ion during the coarse screening phase of biological
macromolecules (proteins, nucleic acids, peptides, and
combinations thereof).
ORDER INFORMATION
ORDER INFORMATION
Description
Pack
Code
Description
Pack
Code
Wizard Precipitant Synergy Screen
192 x 10 mL
MD15-PS-T
Wizard PEG Ion I HT-96
96 x 1.7 mL
MD15-PI14-B
Wizard Precipitant Synergy
Screen HT-96
Wizard PEG Ion 2 HT-96
96 x 1.7 mL
MD15-PI810-B
192 x 1.7 mL
MD15-PS-B
100 mL
EBSR-100-
100 mL
EBSR-100-
250 mL
EBSR-250-
250 mL
EBSR-250-
4
05 Crystal Growth Screens
05 MemMeso™
06 Wizard™ Cubic LCP Kit
06 Wizard Time Screen™
07 Calixar™ V2.0
MEMMESO™
OPTIMIZED SCREEN FOR LCP CRYSTALLIZATION.
MAKE SURE YOU GET THE LIPIDIC PHASE YOU NEED
FOR SUCCESS.
MEMBRANE PROTEINS
This semi-systematic screen has been designed to work in synergy
with Lipidic Cubic Phase (LCP), now commonly used in membrane
protein crystallization, without perturbing phase formation.
MEMBRANE
PROTEINS
MemMeso is presented as a targeted sparse matrix kit of 96 x 10 mL
conditions or 0.25 mL or 1.1 mL in a SBS deep-well block.
With the continued rise of published
membrane protein crystal structures has
come a greater body of evidence of
successful crystallization conditions
and strategies.
Molecular Dimensions provides the largest
range of products for membrane protein
crystal growth data mined from the most
recent publications ranging from MemStart™
to the MemGold™ series to the introduction
of the first specialised additive screen. For in
meso methods a Sponge Phase screen, and
MemMeso™ and Wizard™ Cubic are available
for LCP. Molecular Dimensions supplies the
versatile Laminex™ system for in meso
crystal growth experiments with a choice if
materials and spacer depths.
Features of MemMeso:
n Optimized to work in synergy with Lipidic Cubic Phase (LCP) and the
LCP crystallization method.
n Worry-Free screening in both LCP and Sponge Phase.
n Up-to-date conditions data-mined from current reported GPCR
crystal structures.
n HT-format semi-systematic screening kit, containing 96 conditions
covering a range of pH, precipitants and salt.
n Proven success at crystallizing the crystal structures of eight
membrane proteins, including the structure of channelrhodopsin
(2012, Nature).
Crystals of channelrhodopsin (Kato et al.,
Nature, 2012)
To date, components of MemMeso have crystallized no fewer than
eight membrane proteins structures, namely: Channelrhodopsin (2012,
Nature, Vol 482, p369-375), PfMATE (2013, Nature, Vol 496, p247253), NCX_Mj (2013, Science, Vol 341, p168-172), GkPOT (2013, PNAS,
Vol 110 no 28, p11343-11348), and four bacterial transporters
(manuscript in preparation).
ORDER INFORMATION
Description
Pack
Code
MemMeso™ HT Mini
96 x 0.25 mL MD1-85
MemMeso™ HT-96
96 x 1.1 mL
MD1-87
MemMeso™
96 x 10 mL
MD1-86
MemMeso HT Mini Single Reagent
50 mL
MDSR-80-
Developed in conjunction with the successful laboratory of Prof. Osamu Nureki at the
University of Tokyo, Japan.
5
MEMBRANE PROTEINS
MEMBRANE PROTEINS
WIZARD CUBIC LCP KIT™
WIZARD TIME SCREEN™
THE WIZARD™ CUBIC LCP KIT CONTAINS ALL TOOLS,
FORMULATIONS, AND LIPID TO PREPARE MICROCRYSTALLIZATION EXPERIMENTS ACCORDING TO THE
LCP MICRO METHOD. CUBIC LCP KIT INCLUDES: CUBIC™
SCREEN RANDOM SPARSE MATRIX, SYMMETRIC MIXER
UNION COUPLES TO 50 µL, AND 10 µL SYRINGES ON
BOTH ENDS.
THE WIZARD TOTAL INTEGRAL MEMBRANE PROTEIN
EXTRACTION (TIME) SCREEN AIDS IN THE IDENTIFICATION
OF THE DETERGENT REAGENT THAT SUCCESSFULLY
EXTRACTS A MEMBRANE PROTEIN FROM A MEMBRANE
PREPARATION.
WIZARD™ CUBIC SCREEN
Further analysis can be carried out by ultracentrifugation, and
Fluorescence detection SEC-HPLC.
The Wizard Cubic™ LCP Screen
random sparse matrix is designed
for the crystallization of
biological macromolecules that
are embedded within a lipidic
cubic phase (LCP) host matrix.
Every formulation in this 96-well
plate is compatible with
monoolein-based lipidic cubic
phases. 96 formulations, 1.7 mL
volumes in a 96-well block plate.
One membrane protein target can be screened with one kit.
Accessories
10 μL Microsyringe for use in lipidic cubic phase (LCP) work
250 μL Syringe for the preparation of lipidic cubic phase (LCP).
Repeating Dispenser for Hamilton Syringe
The Hamilton repeating dispenser for delivering liquid volumes of
0.5 µL to 50 µL recommended syringes for use with the repeating
dispenser are the 1700 & 1000 GASTIGHT series and 1700 series
MICROLITRE.
Asymmetric Mixer Union for LCP
This asymmetric mixer union couples to 250 µL syringes on one
side and to 250 µL, 50 µL or 10 µL on the opposing side. This
allows for simple lipidic cubic phase (LCP) generation using two
250 µL syringes and transfer into a 10 µL syringe for small volume
LCP dispensing.
Symmetric Mixer Union for LCP
This symmetric mixer union couples to 50 µL and 10 µL syringes on
both ends. Suitable for lipidic cubic phase (LCP) generation, mixing
and transfer of LCP into 10 µL syringes.
ORDER INFORMATION
Description
Wizard Cubic LCP Kit
MD15-LCP-KIT
Wizard Cubic Screen HT-96 96 x 1.7 mL
MD15-LCP-B
10 µL Microsyringe Pipette for LCP Kit
MD15-LCP-10MSYR
10 µL Syringe
MD15-LCP-10SYR
250 µL Syringe
MD15-LCP-250SYR
Description
Pack
Asymmetric Mixer Union for LCP
MD15-LCP-AUNION
Wizard TIME screen
96 x 0.125 mL MD15-TIME-B
Symmetric Mixer Union for LCP
MD15-LCP-SUNION
100 mL
EBSR-100-
Repeating Dispenser for Hamilton Syringe
MD6-20
250 mL
EBSR-250-
6
Code
ORDER INFORMATION
Code
Protein Production
MEMBRANE PROTEINS
CALIXARENE ADDITIVE FOR CRYSTALLIZATION OF
FUNCTIONAL MEMBRANE PROTEINS FACILITATES
CRYSTALLIZATION THROUGH THE ABILITY OF CALIXAR
DERIVATIVES TO INDUCE ‘SUPRA-MOLECULAR CLUSTERS’
OF MICELLAR-TYPE AND PROMOTE PROTEIN/PROTEIN
INTERACTIONS.
CALIXAR derivatives have been designed & synthesized for their
capacity to generate a network of salt bridges around the protein,
in close proximity to the membrane domain with positivelycharged residues located at the membrane-cytosol interface
of the protein.
Features of Calixar™ V2.0:
n CALIXAR derivatives generate a network of salt bridges around the
protein make hydrophobic contacts and «π-stacking» interactions.
n Enhance protein/ protein interactions and therefore facilitate
crystallogenesis.
n Overcome the defects, drawbacks and obstacles of prior art – such
as detergent-based additive kits and small molecule ‘bullet’ screens.
n Suitable for both membrane and soluble proteins.
DIALYSIS &
ULTRAFILTRATION
CALIXAR™ V2.0
ADDITIVE KIT
NEW
Molecular Dimensions introduces a
completely new range of dialysis and
ultrafiltration devices. Manufactured by
Swissci AG, this novel range of products
combines innovative features with low cost.
For crystallographers the unique dialysis
plate allows microliter samples to be dialysed
against independent solutions offering, for
the first time, crystal growth by dialysis in a
high-throughput configuration.
ORDER INFORMATION
Description
Pack
Code
The CALIXAR™ v2.0 Additive Kit
24 x 50 µL
MD1-95
The CALIXAR™ v 2.0 Additive
Single Reagents
50 µL
MDSR-95-tube number
References
Falson, P. et al. - WO 2010116055, Matar-Merheb R, et al., Protein crystallization additives, use and process.
PLoS One. Mar 31;6(3):e18036
McGovern, R. E et al., (2012)Protein camouflage in cytochrome c–calixarene complexes, Nat Chem 4, 527–
533 (2012)
The Ultrafiltration devices feature fixed dead
stops enabling recovery of as little as 10 µL
samples, ideal for crystallography sample
preparation. The range includes two
centrifuge devices and one passive
concentrator covering a wide range of initial
sample volumes.
The CALIXAR™ v2.0 Additive Kit reagents are manufactured by Calixar SAS and exclusively licensed to
Molecular Dimensions for the manufacture and distribution of this additive kit for final end users only.
7
Protein Production
DIALYSIS
DIACON™
DIACON IS IDEAL FOR GENTLE AND EFFICIENT
BUFFER EXCHANGE, DESALTING AND LIGAND
EXCHANGE OF PROTEIN FROM LOW VOLUME
SOLUTIONS OF UP TO 0.5ML. THE SAMPLE
SOLUTION IS MANUALLY PIPETTED INTO THE
DEVICE WHICH IS THEN HELD UPRIGHT IN
THE DIALYSATE VIA THE FLOATING RACK
PROVIDED, ENSURING THE MEMBRANE IS
ALWAYS IN CONTACT WITH THE DIALYSATE.
The volume of dialysate can be as large as desired; the larger the
volume the larger the concentration differential and therefore a
faster rate of diffusion occurs, optimizing dialysis. Dialysis is
recommended for 2-3 hours and then change the dialysis buffer
leaving for another 2-3 hours. A third dialysis can be carried out if
necessary. Diacon employs specially selected regenerated
cellulose membranes.
Features of Diacon:
n Ready to use, just add sample.
n Ideal for small sample volumes up to 0.5 mL.
n No cross contamination or leakage.
n Regenerated cellulose membrane; sulphur
and heavy metal free.
n Molecular weight cut off of 10,000 Daltons.
n Excellent sample recovery without disturbing
valuable protein complexes.
n Dialysis complete in 2-4 hours.
n Gentle and efficient desalting.
n Caps to prevent spillage/evaporation.
n Floating tube rack provided holds up to 18 Diacon at any one time.
n Single use - disposable device; no cleaning required.
DIATUBE
™
Diatube holds up to a 20 mL sample solution one side of the dialysis
membrane and up to 25 mL dialysate on the other, creating a
concentration differential. Diatube is ideal for buffer exchange and
desalting of sample volumes from 5 to 20 mL. The dialysate can be
changed at any point to recreate the concentration differential for
the dialysis process to continue.
The disposable Diaplate is a 96 well micro-dialysis plate for very
small volumes of up to a maximum of 3.2 µL. The sample solution is
pipetted onto each of the 96 membranes with up to 0.35 mL unique
dialysis solutions in the wells used for exchange. Dialysis is
recommended for 2-3 hours and then the dialysis buffer is changed,
left for another 2-3 hours to achieve over 90% exchange. Further
dialysis steps can be carried out as required. Incubation on a rocking
table for speed and a moisture chamber for full humidification can
be applied to the experiment if desired. The Diaplate™ kit includes:
Diaplate with 200 Micron Pressure Adhesive Spacer, 200 Micron
UVP Cover Film, engraved UV Protective Cover and a sealing Paddle.
Is this your NEW crystallization plate?
Owing to the high position of the membrane and the UV transparent
cover, samples on Diaplate can be observed by microscopy,
fluorimetry, turbidimetry and light scattering. Diaplate is therefore
ideal for crystallization by dialysis by changing ionic strength, or
simply offering different kinetics to vapour diffusion. Diaplate is also
suitable for ligand exchange, slow controlled addition of
cryoprotectant and for protein stability screening.
Features of Diaplate:
n Simple handling, ready to use, easy sample recovery.
n Complete kit, no additional materials required.
n Pipetting can be performed manually or using a robot.
n Ideal for the desalting of up to 3.2 µL protein solution.
n No cross contamination or leakage between wells guaranteed.
n 96 individual regenerated cellulose membranes; sulphur and heavy
metal free.
n Molecular weight cut off of 10,000 Daltons.
n Protein crystallisation applications allow for unique matrix changing.
n Low protein binding and high protein retention.
Features of Diatube:
n Dialysis complete in 2-4 hours.
n Exceptionally easy to use, just add sample
n Gentle and efficient desalting without disturbing valuable
protein complexes.
n Ideal for large sample dialysis up to 20ml
n No cross contamination or leakage
n Membrane tubing skin made of regenerated
cellulose membrane; sulphur and heavy
metal free
n Excellent sample recovery without disturbing
valuable protein complexes
n Large surface area of membrane resulting in
rapid dialysis
n High throughput; simultaneous processing of 96 experiments.
n Reliable and uniform, stable construction.
ORDER INFORMATION
Description
Pack
Code
Diaplate 96 Sample 3.2 µL Dialysis Plate 3
MD6-70
n Caps to prevent spillage/evaporation
Diacon 0.5 mL Dialyser
40
MD6-71
n Single use - disposable device; no cleaning required
Diatube 20 mL Dialyser
20
MD6-72
n Stable construction, pipette included
8
DIAPLATE™
Diacon, Diatube and Diaplate are manufactured by Swissci AG.
CEROCON™
CEROCON COMES READY TO
USE IN A 2 ML MICROCENTRIFUGE TUBE WITH A
SELF-LOCK LID. ONCE
LOADED WITH SAMPLE
SOLUTION IT MAY BE
CENTRIFUGED TO A
MAXIMUM OF 10,000G.
The device has a concentration fixed dead-stop of 10 µL to avoid
sample drying, which is achieved in approximately 20 minutes.
Protein Production
ULTRAFILTRATION
URICON™
Uricon is a static concentrator used for the
rapid concentration of protein solutions.
Up to 15 mL sample solution is pipetted
into the isolated chamber within the device
where one inner surface is a membrane of
selective permeability. The membrane is
backed by a pressure resistant absorbent
pad and gel that pull the solvent and salts
through the ultrafilter by absorption,
concentrating the sample. Uricon has an
impermeable dead stop at the base of the
unit which will prevent the sample from
completely drying out so that sample
recovery can take place.
Features of Uricon:
Features of Cerocon:
n Ideal for small sample volumes up to 0.5 mL.
n No cross contamination, enclosed unit.
n Highly hydrophilic membrane surface allows excellent product
recovery even with low molecular weight proteins.
n Reverse spin recovery; inverted design allows very high protein
recovery rates.
n Static concentrator; exceptionally easy to use, just add sample.
n Ideal for sample volumes up to 15 mL.
n Graduations on the device for smaller sample volumes and
concentration factors.
n No cross contamination, single cell device.
n Highly hydrophilic membrane surface allows excellent product
recovery.
n Ultra low binding membrane allows rapid proportionate
concentration of proteins.
n Ultra low protein binding membrane allows rapid, proportionate
concentration of proteins.
n Concentration of 0.5 mL to 0.01 mL in one step.
n Convenient storage for filtrate or concentrate.
n Absorbent draws solvent and salts through the ultrafilter leaving a
concentrated sample.
n Reliable and highly reproducible.
n No refilling necessary to achieve a concentration factor up to 300x.
n Cap to reduce possibility of contamination, evaporation, odours
and spillage.
n Fixed dead-stop prevents the drying out of sample.
n Side clips to link desired quantity of Uricon devices together.
n Low hold-up-volume, stable construction.
CONVERSE™
The reverse direction of flow of
Converse compared to standard
ultrafiltration combined with the
high pressure exerted during
centrifugation leads to rapid
processing times and excellent
sample recovery. Converse L is
ideal for larger sample solutions
of up to 20 mL and Converse S is
suitable for smaller sample
solutions of up to 6 mL.
n Fixed dead stop (50 µL) prevents sample drying out.
ORDER INFORMATION
Description
Pack
Code
Cerocon 0.5 mL Concentrator 3kDa MWCO
40
MD6-50
40
MD6-51
40
MD6-52
40
MD6-53
Converse L 20 mL Concentrator 3kDa MWCO
20
MD6-54
20
MD6-55
20
MD6-56
n No cross contamination, enclosed unit.
20
MD6-57
n Specially selected low absorption membranes in inverted design for
optimum recovery.
Converse S 6 mL Concentrator 3kDa MWCO
20
MD6-58
20
MD6-59
20
MD6-60
Features of Converse:
n Ready to use, just add sample.
n Ideal for both large and small sample volumes.
n Novel recovery spin option with very high protein recovery rates.
n Ultrafiltration is reversed preventing any membrane blockage even
with particle laden samples.
20
MD6-61
n Converse orientation results in very rapid filtration.
Uricon 15 mL Concentrator 3kDa MWCO
25
MD6-62
25
MD6-63
25
MD6-64
25
MD6-65
n Large dead stop, stable construction.
Cerocon, Converse and Uricon are manufactured by Swissci AG.
9
SITTING DROP PLATES
COMBICLOVER™ 500
CRYSTALLIZATION PLATES
UTILIZES A PATENTED SITTING-DROP WELL DESIGN. EACH
PLATE HAS 24 OF THE DESCRIBED CLOVERS, PROVIDING 96
FLAT-BOTTOM SITTING DROP CHAMBERS. THE UNIQUE
DESIGN ALLOWS FOUR DIFFERENT CRYSTALLIZATION
EXPERIMENTS TO OCCUR WITHIN EACH CLOVER PROVIDING
MAXIMUM EFFICACY AND CONSERVATION OF
CRYSTALLIZATION SOLUTIONS.
Features of the CombiClover 500 Crystallization Plate:
n Plate dimensions are 85.5 mm x 127.8 mm x 14.4 mm.
RIGAKU
CRYSTALLIZATION
PLATES
n Plates are compatible with SBS (Society for Biomolecular Screening)
standard laboratory automated instrumentation.
n Drop chambers are 9 mm apart and reservoir chambers are 18 mm
apart in both horizontal and vertical directions for easy use with
multi-channel pipettors.
n The central reservoir volume is 500 microliters.
n The satellite drop chamber volume is 10 microliters.
n Formerly called CombiClover Junior.
A CENTRAL RESERVOIR IS
CONNECTED TO FOUR SATELLITE
DROP CHAMBERS VIA DEDICATED
VAPOUR DIFFUSION CHANNELS TO
CREATE A NOVEL COMBINATORIAL
"CRYSTALLIZATION CLOVER".
CombiClover 500 Crystallization Plate.
10 Rigaku Crystallization Plates
10 Combiclover™ 500
Crystallization Plates
11 Compact™ 300
11 Combiclover™ 4 Chamber
COMBICLOVER™ 1500
THE COMBICLOVER 1500 CRYSTALLIZATION PLATES
OFFER THE EXACT SAME DESIGN AND UTILITY AS OUR
COMBICLOVER 500 CRYSTALLIZATION PLATES, BUT
OFFER LARGER CAPACITY IN THE RESERVOIR AND DROP
CHAMBERS. EACH PLATE HAS 24 OF THE DESCRIBED
CLOVERS, PROVIDING 96 FLAT-BOTTOM SITTING
DROP CHAMBERS.
Features of the CombiClover 1500 Crystallization
Plate:
n The central reservoir volume is 1.5 milliliters.
n Formerly called CombiClover.
10
COMPACT™ 300
THE COMPACT 300 CRYSTALLIZATION PLATE UTILIZES
THE PATENTED SITTING DROP WELL DESIGN AND VAPOUR
DIFFUSION CHANNEL. THIS PLATE HAS 96 RESERVOIRS
WITH 96 DROP CHAMBERS.
Features of the Compact 300 Crystallization Plate:
n Plates are compatible with SBS (Society for Biomolecular Screening)
standard laboratory automated instrumentation.
n Drop chambers are 9mm apart in both horizontal and vertical
directions for easy use with multi-channel pipettors.
SITTING DROP PLATES
COMBICLOVER™
4 CHAMBER
Sitting Drop Plates
SITTING DROP PLATES
THE COMBICLOVER 4 CHAMBER CRYSTALLIZATION PLATE
UTILIZES FOUR PARTITIONED RESERVOIR CHAMBERS IN THE
PLACE OF ONE CENTRAL RESERVOIR. THESE FOUR
PARTITIONED CHAMBERS ARE EACH CONNECTED TO A
SATELLITE DROP CHAMBER VIA DEDICATED VAPOUR
DIFFUSION CHANNELS TO CREATE A VARIATION OF THE
NOVEL COMBINATORIAL "CRYSTALLIZATION CLOVER"
PROVIDED IN THE PLATES. EACH PLATE HAS 24 OF THE
PARTITIONED RESERVOIRS AND 96 FLAT-BOTTOM SITTING
DROP CHAMBERS. THIS DESIGN ALLOWS FOUR DIFFERENT
EXPERIMENTS TO BE CONDUCTED IN EACH CLOVER, WITH
EACH PORTION CONTAINING A DIFFERENT REAGENT, AND
ALLOWING THEM TO BE INVESTIGATED SIMULTANEOUSLY.
n The reservoir volume is 300 microliters.
n The drop chamber volume is 6 microliters.
n Formerly called Compact Junior.
Features of the CombiClover 4 Chamber Crystallization
Plate:
n The capacity of each of the four partitioned areas of the reservoir is
250 microliters.
n Formerly called CompactClover.
Compact 300 Crystallization Plate.
CombiClover 4 Chamber Crystallization Plate.
ORDER INFORMATION
Description
Pack
CombiClover 1500 Crystallization Plates 10
CombiClover 1500 Crystallization Plate.
Code
MD15-CBT-10
MD15-CBT-40
MD15-CJR-10
MD15-CJR-40
Compact 300 Crystallization Plates
10
MD15-XJR-10
Compact 300 Crystallization Plates
40
MD15-XJR-40
CombiClover 4 Chamber
10
MD15-XPT-10
CombiClover 4 Chamber
40
MD15-XPT-40
11
IN LAMINA PLATES
LAMINEX™ HT
GLASS BASE
LAMINEX HT GLASS BASES ARE AVAILABLE WITH 100
OR 200 MICRON SPACER. SPECIALLY CUT SBS SIZE
LAMINEX GLASS BASES.
THE FRAME-LESS VERSION OF LAMINEX- IDEAL FOR
COMPACT IMAGING HOTEL CONFIGURATIONS.
HIGHTHROUGHPUT
PLATES
FOR LIPIDIC CUBIC AND SPONGE
PHASE CRYSTALLIZATION
Features of the Laminex HT Glass Base:
n Specially cut SBS size Laminex glass bases for Lipidic Cubic Phase
experiments.
n Sized for frame-less handling on robotic beam-lines or in automated
imaging systems. Additional space provides room for barcode label
on upwards facing surface.
n Compatible with imaging systems that use gripper arms for handling
plates without a frame.
n Dispense as with Standard Laminex and seal with standard Laminex
cover options.
12 High-throughput plates
12 Laminex™ HT Glass Base
12 Laminex™ UV Plastic 200
micron Film Cover
Laminex™ UV Plastic 200 micron Film Cover
Laminex™ UV Plastic Film Covers 200 micron thick. Replaces
MD11-53 700 micron thick plastic covers.
There has been some speculation that water vapour may evaporate
through a thin film cover. As yet there is no experimental evidence
to substantiate this suggestion. Users have also speculated that it
may be related to how well the film is pressed down onto the
adhesive spacer.
Crystal growth plate design has evolved considerably
over the past decade to incorporate excellent optical
properties for imaging experiments; plastic surfaces to
address drop behaviour; birefringence; hydrophobicity;
UV transparency and well geometry for ease of
harvesting crystals. Molecular Dimensions high quality
moulded plates, manufactured by Swissci, have no flow
lines and no bubbles and feature all of the above
qualities for sitting drop, hanging drop, microbatch,
counter diffusion and versatile sandwich devices for LCP.
12
We now include a sample of the 200 micron cover film for you
to try and compare in our starter kits. At the same time we will be
discontinuing the original solid (700 micron) plastic cover as
experience has shown that very few people use it.
ORDER INFORMATION
Description
Pack
Code
Laminex™ HT Glass Base 200 Micron
10
MD11-50-HT
Laminex™ HT Glass Base 100 Micron
10
MD11-50-100-HT
Laminex™ UV Plastic 200 micron
Film Cover
10
MD11-53-200
CRYOCRYSTALLOGRAPHY
THE INTRODUCTION OF STANDARDS FOR SAMPLE MOUNTING AND
ROBOTIC SAMPLE CHANGERS HAS FUELLED BOTH HIGHER
THROUGHPUT AND HIGH QUALITY DATA COLLECTION
13 Cryocrystallography
14 Actiloops™
15 Automatic Sample Changer
16 Cryosol™
16 Dilvac Dewers
The introduction of standards for cryogenic sample mounting combined with high
precision components, machine made polymer film loops and robotic sample
changers has fuelled both higher throughput and high quality data collection.
Molecular Dimensions offers both ESRF/EMBL pucks and Uni-Pucks and accessories
commonly used in robotic sample changers. Your cryoprotection needs are also
addressed with two optimised reagent kits CryoProtX™ and now CryoSol™.
LithoLoop™ technology has been further developed to offer you the most advanced
cryoloop design and performance with 3D ActiLoops™. Not only are these new loops
made from a technically superior 3D polymer film they also incorporate a drainage
channel proven to remove excess mother liquor from the crystal and improve
data quality.
13
Loop diameter vs Resolution
™
ACTILOOPS
Resolution (Å)
Cryo Crystallography
ACTILOOPS
INTRODUCING THE MOST ADVANCED CRYO SAMPLE MOUNT
ACTILOOP™ WITH “ACTIVE-DRAIN™” TECHNOLOGY.
TECHNICALLY SUPERIOR 3D POLYMER FILM FOR MINIMAL
BACKGROUND AND MAXIMUM STRENGTH TO PREVENT
LOOP MOTION IN THE COLD STREAM. ACTIVE-DRAIN
CONSTRUCTION ENHANCED WITH HYDROPHILIC POLYMER
SURFACE TREATMENT REMOVES EXCESS MOTHER LIQUOR
FROM THE CRYSTAL FOR LOWER BACKGROUND AND
SUPERIOR RESOLUTION.
1.9
LithoLoops
1.85
1.8
1.75
ActiLoops
1.7
0.2
0.4
0.6
Loop diameter (mm)
Drop volume (µL)
Loop diameter vs Drop volume
0.04
LithoLoops
0.03
ActiLoops
0.02
0.01
0
0.2
0.4
0.6
Loop diameter (mm)
ActiLoops are available precision mounted in 18mm pins or
in SPINE CryoCaps™ and magnetic CryoVials as ActiLoop
CryoMount™ Sets.
ORDER INFORMATION
Description
Pack Mounted
CryoMount
Set
Round, Elliptical or Basket Loops +Groove +Reservoir
Round ActiLoops 0.08 mm
20
MD7-633
MD7-633CM
The Elliptical loops are slanted with 10 degrees tilted from a Z-axis.
20
MD7-634
MD7-634CM
20
MD7-635
MD7-635CM
20
MD7-636
MD7-636CM
20
MD7-637
MD7-637CM
X-ray source: RIGAKU RAXIS7/FR-E
20
MD7-638
MD7-638CM
Temperature: 100K
Elliptical ActiLoops 0.02 x 0.3 mm
20
MD7-639
MD7-639CM
Distance: 150 mm
20
MD7-640
MD7-640CM
20
MD7-641
MD7-641CM
Structure Description
Results of test on the drainage
effect in ActiLoops versus LithoLoops
X-ray diffraction conditions
Exposure time: 5 minutes
Δ Φ: 0.5°
14
Basket ActiLoops 0.08 mm
20
MD7-642
MD7-642CM
Protein crystal and buffers
20
MD7-643
MD7-643CM
HEWL (0.2mm X 0.2mm x 0.15mm)
20
MD7-644
MD7-644CM
Reservoir solution: 50mM Na acetate pH4.4, 1M NaCl
20
MD7-645
MD7-645CM
Cryobuffer: 50mM Na acetate pH4.4, 1M NaCl, 22.5% ethylene
glycol
20
MD7-646
MD7-646CM
20
MD7-647
MD7-647CM
Cryomount loops
Elliptical Basket ActiLoops
0.02 x 0.3 mm
20
MD7-648
MD7-648CM
ActiLoops: loop diameter = 0.3 mm, 0.4 mm, 0.5 mm
film thickness = 25 mm (bottom layer), 25 mm
(top layer)
0.05 x 0.5 mm
20
MD7-649
MD7-649CM
LithoLoops: loop diameter = 0.3 mm, 0.4 mm, 0.5 mm
film thickness = 25 mm
0.1 x 0.5 mm
20
MD7-650
MD7-650CM
AUTOMATIC SAMPLE CHANGER
UNI-PUCKS AND
ACCESSORIES
THE UNIVERSAL V1-PUCK (UNI-PUCK) IS A SAMPLE PIN
STORAGE AND SHIPPING CONTAINER FOR USE WITH THE
MAJORITY OF AUTOMATED SAMPLE MOUNTING SYSTEMS
WORLDWIDE (DIAMOND, SOLEIL, SPRING-8, PHOTON
FACTORY, CLSI AND ACROSS THE USA).
CONTENTS OF THE STARTER KIT
7 x Uni-Pucks
The Universal V1-Puck (uni-puck) is a sample pin storage and
shipping container compatible with the majority automated sample
mounting systems worldwide. Each Uni-puck holds 16 standard
18 mm sample pins in CryoCaps. It consists of two parts: 1) the
sample enclosure, and 2) the sample base piece. Each part of the
Uni-puck has a unique serial number for identification. Custom
Colours and Serial Numbers are available upon request. (Always
consult your local beamline user support contact about specifics of
container and sample pin compatibility).
1 x Puck-Shelved Shipping Cane
Seven uni-pucks may be shipped or stored in this cane, which fits
into the CX100 transport Dewar (Dry Shipper - MD7-21).
1 x Angled Cryo-Tongs
These tongs are designed specifically to hold and transfer cryocooled pucks.
1 x Puck Separator Tools
This set of tools includes a base removal platform and a porcupine
push tool to recover your samples.
1 x Puck Wand
Magnetic puck-base holder; it is designed for attaching the base
plate to the sample enclosure.
1 x Puck Dewar Loading Tool Set
ORDER INFORMATION
This tool is used to separate uni-pucks to load them into the robot
dewar. It consists of two pieces: a Teflon tube part and a metal rod
part.
Description
Pack
Code
Uni-Puck Starter Kit
1
MD7-600
Uni-Puck
1
MD7-601
1 x Puck Loading Dewar with Lid
Uni-Puck 7 Pack
7 pucks
MD7-602
This dewar has space to hold up-to three uni-pucks for ease of
loading.
Puck-Shelved Shipping Cane
1
MD7-603
Angled Cryo-Tongs
1
MD7-604
Puck Separator Tools
1 set
MD7-605
Puck Wand
1
MD7-606
Puck Dewar Loading Tools
1 set
MD7-607
Puck Loading Dewar with Lid
1
MD7-608
Lifting hook
1
MD7-609
Uni-Puck Carry Case
1
MD7-610
15
CRYOPROTECTANTS
DEWARS
CRYOSOL™
DILVAC DEWARS
CRYOSOL IS AN EXTENDED SET OF MULTICOMPONENT
SOLUTIONS FOR CRYSTAL CRYOPROTECTION AND
SOAKING OF HYDROPHOBIC LIGANDS INTO CRYSTALS.
IN ADDITION TO OUR RANGE OF FOAM AND POPE
ALUMINIUM DEWARS MOLECULAR DIMENSIONS
NOW OFFER THESE STAINLESS STEEL DEWARS ON
FAST DELIVERY.
Features of CryoSol:
Blue polypropylene container with handle and lid 1 L
n Use in co-crystallization screening of protein-ligand complexes.
This represents the complete answer in low-cost, high efficiency
Dewar flasks. Utilising a proven borosilicate glass inner vessel,
MD7-807 features a tough, blue plastic, shock-absorbing
container with many safety features. The unit is easily dismantled
to facilitate refill changes and includes a coated handle and a
'push-fit' insulated lid, vented for safe handling of all liquid gases.
n Preparation of ligand-loaded cryosolutions for soaking experiments.
n Ideal for the solubilization of hydrophobic ligands.
n Protein friendly mixes of DMSO/dioxane/ethylene glycol should
improve ligand solubilization.
n Contains 2,3-butanediol- compatible with enzymatic activity.
n Mixed solutions can be used as an additive screen (up to 5% in
precipitant).
n Compliments CryoProtX™.
Stainless steel container with clamp lid attachment,
vent and handle. 1 L, 2 L & 4.5 L capacity
Fitted in highest-quality stainless steel containers, therefore rustfree, anti-corrosive, all hygienic and strong. These Dewars have an
all stainless steel lid with self-contained insulation. Toggle-clamps
secure the lid to the body for safe transportation.
Dimensions 1 L (dia x height) 116 mm x 228 mm.
Dimensions 2 L (dia x height) 142 mm x 268 mm
Dimensions 4.5 L (dia x height) 182 mm x 350 mm
*replacement glass is available for this product- just contact us for details.
The following reference gives an example of the use of CryoSol in
crystallization of glycoconjugated MMP-inhibitor complexes. The
sugars were solubilizing the complex too much so Dioxane was
added to the PEG to crystallize it.
Ciccone L., Vera L., Tepshi L., Rosalia L., Rossello A. & Stura
E.A.(2015) Multicomponent mixtures for cryoprotection and ligand
solubilization. (ICCBM15 poster) Biotechnol. Reports submitted.
Preprint available on request.
CrySol is presented as 33 x 1.5 mL microtubes (18 x multi-component
cryoprotectant solutions (CM1-18), 6 x solubilisation solutions (SM16) and 9 x stock solutions).
ORDER INFORMATION
Description
Pack
CryoSol
33 x 1.5 mL MD1-90
The Cryo Combination
CryoProtX Single Reagent
Code
MD1-94
1.5 mL
MDSR-61-
References:
Ciccone L, Tepshi L, Nencetti S, Stura EA.N Biotechnol. (2015) “Transthyretin complexes with curcumin and
bromo-estradiol: Evaluation of solubilizing multicomponent mixtures” 32(1):54-64.
L Vera, EA Stura - Crystal Growth & Design, (2014) “Strategies for Protein Cryocrystallography”,14 (2),
427-435.
CryoSol was developed and formulated in the laboratory of Dr. Enrico Stura; CryoSol™ and CryoProtX™ are
manufactured and distributed under an exclusive licence with CEA Life Sciences Division, Saclay, France.
16
CRYSTALLOGRAPHY
INSTRUMENTATION
Shallow form stainless steel container, no handle no lid
0.57 L, 1 L & 2 L capacity
Shallow form, no handle or lid.
Wide-mouthed shallow glass Dewars,
Dish-shaped, fitted in stainless steel protective containers.
The shallow form configuration allows for minimum amount of
coolant to be used.
Dimensions 0.57 L (dia x height) 150 mm x 95 mm
17 Crystallography Instrumentation
18 Microscopes
20 Uvex UV Fluorescence
Imaging Systems
*replacement glass is available for this product- just contact us for details.
ORDER INFORMATION
Description
Pack
Code
Blue polypropylene container with
handle and lid 1 L
1
MD7-807
Stainless steel container with handle
and lid 1 L capacity
1
MD7-808
and lid 2 L capacity
1
MD7-809
and lid 4.5 L capacity
1
MD7-810
Shallow form stainless steel container,
no handle no lid 0.57L capacity
1
MD7-811
no handle no lid 1 L capacity
1
MD7-812
no handle no lid 2 L capacity
1
MD7-813
Molecular Dimensions is well known
for supplying high quality crystal
growth incubators whether it be
bench-top or floor standing models.
We are now pleased to be able to
offer a range of high quality low cost
microscopes ideal for the
crystallography laboratory. Or for
those looking for a more automated
imaging system we have teamed up
with Jan Scientific to supply the UVEX
UV Fluorescence Imaging Systems.
17
Crystallography Instrumentation
18
MICROSCOPES
HIGH QUALITY, AFFORDABLE STEREOMICROSCOPES
TO SUIT EVERY NEED:
Features:
n Large, sturdy bases for working with crystallization plates
n Between 7x and 97.5x zoom magnification range with extra wide-field
eyepieces ideal for all needs
Choose from:
• In-built incident and transmitted light
• Light-box with mirrored base
n Large working distance for ease of crystal manipulation and freezing
n Ergonomic adjustment allowing use by a large number of people
n Trinocular head for camera attachment
n Optional polarizer
MD5-912 Trinocular Microscope with 7x - 45x zoom with
incident and transmitted light
incident and transmitted light
n Built-in Illumination providing both incident and transmitted light –
cool LED lower lamp, upper halogen spot lamp.
n Built-in Illumination providing both incident and transmitted light –
cool LED lower lamp, upper halogen spot lamp.
n 7x - 45x zoom magnification range with 10x extra wide-field
eyepieces, ideal for crystal detection.
eyepieces, ideal for crystal detection.
n 118 mm working distance for ease of crystal manipulation.
n Large base for working with crystallization plates.
n Precision, smooth focus system.
n Trinocular head with optional c-mount adaptor for camera
attachment.
attachment.
n Optional Polarizer.
n Optional Polarizer.
n Optional 15x eyepieces to give 10.5x – 67.5x zoom magnification.
n Optional 15x eyepieces to give 15x – 97.5x zoom magnification.
n Optional reticle (micrometer) eyepieces to measure the size of your
crystals
n Optional reticle (micrometer) eyepieces to measure the size of
your crystals.
Light Box and Mirrored base
Light Box and Mirrored base
eyepieces.
eyepieces.
n Ideal for detection of microcrystals.
n Ideal for detection of microcrystals.
n Halogen light box with adjustable mirror base for ease of
crystal detection.
n Halogen light box with adjustable mirror base for ease of
crystal detection.
attachment.
n Trinocular head with optional c-mount adaptor for
camera attachment.
n Optional adjustable upper LED ring light (MD5-909).
n Optional adjustable upper LED ring light (MD5-909).
n Optional 15x eyepieces to give 10.5x – 67.5x zoom magnification.
n Optional 15x eyepieces to give 15x – 97.5x zoom magnification.
your crystals.
your crystals.
ACCESSORIES
Description
Code
Detachable, rotatable polarizer for identifying
crystals using birefringence
MD5-919
Pair of 15x eyepieces for amplified magnification
MD5-916
10x focussing eyepiece with 10 mm reticle for
MD5-917
determining crystal size
15x focussing eyepiece with 10 mm reticle for
determining crystal size
MD5-918
Adjustable intensity 60 LED ring illuminator
with separate power controller
Long working life with no heat
MD5-909
C-mount adaptor for camera attachment
MD5-920
CCD Camera for capturing high quality images
MD5-102CCD
19
Crystallography Instrumentation
UVEX UV
FLUORESCENCE
IMAGING SYSTEMS
SENSITIVE, HIGH CONTRAST UV FLUORESCENCE
MICROSCOPE FOR SIMPLE DIFFERENTIATION OF PROTEIN
OR OTHER BIOMOLECULES FROM SALT CRYSTALS. FULL
RANGE AVAILABLE FROM MANUAL TO FULLY INTEGRATED,
TEMPERATURE CONTROLLED HOTEL SYSTEMS.
UVEX-m manual UV fluorescence microscope for
protein crystal detection
A very sensitive, high contrast UV fluorescence microscope.
6 megapixel cooled monochrome camera.
Deep UV optimized optics.
LED light sources for long life and no heat.
Field of view up to 3.5 mm.
Key Features:
Resolution as high as 200 nanometer per pixel for detection of
microcrystals.
n UV fluorescence:
Robust with a compact footprint.
• In-situ protein-salt discrimination.
Easy to learn to use and operate.
• Discovery of hidden crystals e.g. in LCP.
• Microcrystal identification.
n UV absorbance/transmission
Options:
High-magnification objectives for detecting microcrystals.
• Recognition of nucleic acid crystals.
Manual XY stage.
• Detection of protein crystals where fluorescence may be quenched
by adjacent charged residues, ligands, or chromophores.
Crossed polarizers.
n Crossed polarizer - ideal for LCP.
n Z-slicing with extended focus imaging.
UV transmission.
Motorized Z for imaging Z-slices and building a composite ‘extended
focus’ image.
n Suitable for sitting drop, hanging drop, LCP and free diffusion
capillary experiments.
n Excellent image quality – from a combination of a highly UV sensitive
camera, reduced noise, custom optics and LED light sources.
n Resolution down to 0.2 microns per pixel.
CUSTOMIZABLE - ASK FOR MORE INFORMATION
Beat Blattmann, University of Zurich:
"We have had our JANSi UVEX brightfield/UV fluorescence microscope
for over 2 years and have inspected >150,000 protein crystallization
experiments in that time. In our constantly changing multi-user
environment, our UVEX microscope has performed very reliably over
that time span and was key to success of several research projects."
20
UVEX-p Plate-scanning UV fluorescence microscope
for protein crystal detection
A very sensitive, high contrast UV fluorescence microscope.
6 megapixel cooled monochrome camera.
Deep UV optimized optics.
LED light sources for long life and no heat.
Field of view up to 3.5 mm.
Resolution as high as 200 nanometer per pixel for detection
of microcrystals.
Robust with a compact footprint.
Easy to learn to use and operate.
Browser-based image management software.
Automated XY-stage.
Z-slicing with extended focus imaging.
Options:
n Crossed polarizer.
n UV absorbance/transmission.
n Additional wavelengths.
n Additional objectives.
n Complete integration with a range storage hotels.
• Storage capacity (SBS) – 100, 250, 500.
• Can be combined with VDX/XRL and LCP sandwich plates.
• Vibration isolated; vibration minimized plate handling.
• Sensors to detect the presence of plates and avoid collision.
• Ambient temperature to 4° C cooling option.
• Cooling design avoids transmission of vibration from cooler.
• User specified plate scan schedule; may be modified at any time
from web browser-based, remote image management software.
CUSTOMIZABLE - ASK FOR MORE INFORMATION
Dimensions:
12" x 12" x 18"
Weight:
30 lb
Electrical:
110 - 240 V; 50 - 60 Hz; 600 W max
Light sources: All LED, pulsed on-demand, instant on, instant off
Optics:
Nominally 5x and 15x objectives standard, other
magnifications optional
MD5-800
Optical:
Resolution as low as 0.2 µm/pixel;
coverage area 3.5 mm
MD5-805
Detector:
6 megapixel cooled monochrome camera with high
UV sensitivity
Description
Code
UVEX-m manual UV fluorescence microscope
for protein crystal detection
UVEX-p plate-scanning UV fluorescence
microscope for protein crystal detection
21
How to order
You can place your order online, by fax, telephone or via e-mail. Our site includes a full e-commerce
facility for selecting and ordering your product. Credit card orders can be placed securely on-line.
Returns
Returns will not be accepted without prior authorization. Damaged items must be notified within 7 days.
Prices
Prices are subject to change without notice. There is no minimum order charge.
To place an order in Europe or the rest of the world, excluding the
Americas please place your order with our UK office.
To place an order in the USA, Canada, Mexico or South America please
place your order with our USA office.
Molecular Dimensions Ltd.
Unit 6, Goodwin Business Park,
Willie Snaith Road, Newmarket, Suffolk, CB8 7SQ,
UK
Telephone: +44 (0)1638561051
Fax: +44 (0)1638660674
Molecular Dimensions Inc.
849 Sunshine Lane, Altamonte Springs, Florida, 32714
USA
Telephone: +1 877 479 4339
or +1 407 886 6901
Fax: +1 321 972-8896
To order by e-mail use
[email protected]
To order by e-mail use
[email protected]
To order on account please contact us to set up an account.
To order on account please contact us to set up an account.
Shipping
Shipping
Products are shipped by DHL.
Products are shipped by Fedex.
Distributors
CHINA
LBD Life Sciences China Limited
Unit 211, Building 1, No 1133 Halei Road,
Zhangjiang High-tech Park, Pudong District,
Shanghai, P.R. China
Tel: +86 21 51973610
Fax +86 21 50478211
E-mail: [email protected]
Website: www.lbd-life.com
Seaskybio Technology Co.Ltd
Beijing Office
Room 904A Building,
Maples International Centre,
No. 32 Xizhimen North Street,
Haidian District, Beijing, P.R. China, 100082
Tel: +8610-62220605
Fax: +8610-62168941
Website: http://www.seaskybio.com/index.html
Shanghai Office
Floor 12, B Building, Peng Pu Mansion,
No 3155, Gonghe New Road, Zhabei District,
Shanghai, China.
Tel: +8621-66520678
Fax: +8621-54481170
Email: [email protected]
Website: http://www.seaskybio.com/index.html
Bioray Instruments Limited
Rooms 706 Building No.4 Section No.336
Huilongguan East Ave. Changping, Beijing, China,
102206
Tel: +8610-52882746/+8610-52882748
Fax: +8610-60728319
Web: www.biorayinstruments.com
BeiJing RJ-bio Technology Co.Ltd
2th floor, Building 89, Zhongguancun
East Road, Haidian District, Beijing.
Tel: 010-82805432
Fax: 010-82805432
Web: www.rj-bio.com
INDIA
TTP Labtech Ltd.
Tel: 00-91-9910397385 or 00-91-9968291292
Email:[email protected]
JAPAN
Waken B Tech, Co., Ltd.
Sales Promotion Dept.
1-12-8 Senbahigashi, Minoh-city,
Osaka 562-0035, Japan
Phone: +81-72-749-5300 Fax: +81-72-749-5600
Website: http://www.wakenbtech.co.jp
KOREA
MDxK Molecular Diagnostics Korea Inc.
5F, 80 Nonhyeon-ro, Gangnam-gu (Gaepo-dong,
Jisung B/D), Seoul, 135-964, Korea
Tel. +82 2 578 8848
Fax. +82 2 578 8838
Website: http://www.mdxk.co.kr/
MALAYSIA
Eko-Teknik
15-01Wisma Zelan, No.1 Jalan Tasik Permaisuri 2
Bandar Tun Razak, 56000 Kuala Lumpur
Malaysia
Tel: +603 91736440
Fax: +603 91736451
Website:http://www.eko-teknik.com/
RUSSIA
Vladimir Chernyavski
Tel. +7-916-757-57-87
SINGAPORE
Practical Mediscience Pte Ltd
115A Commonwealth Drive #04-19
Singapore 149596
Tel: 62542633
Fax: 62543301
Website: http://www.practicalmediscience.com/
Bio-Etc Pte Ltd.
41 Science Park Road, #04-06A The Gemini
Singapore Science Park II, Singapore 117610
Mobile: +65 8617 7360
Office: +65 6779 4149
Fax: +65 6779 4095
Website: http://www.bio-etc.com/singapore/
TAIWAN
Yeong Jyi Chemical Apparatus Co., Ltd.
1FL. No.45, San Yang Road,
Sanchung City 241, Taipei, Taiwan.
Website: http://www.yjcacl.com
Tel:886 2 29828578
Fax:886 2 29897897
E-mail: [email protected]

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