Get Clear Idea About Elisa Test Procedure

Get Clear Idea About Elisa Test Procedure
@ Antibody-Elisa.com
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At Antibody-Elisa you will find the principle and
method of ELISA. Enzyme-linked immunosorbent
assay (ELISA) is a method of target antigen (or
antibody) capture in samples using a specific
antibody (or antigen), and of target molecule
detection/quantitation using an enzyme reaction
with its substrate. Elisa
Test
Procedure an
enzyme-linked immunosorbent assay is a test that
detects and measures antibodies in your blood.
This test can be used to determine if you have
antibodies related to certain infectious conditions. Usually an ELISA test is used to diagnose:
HIV, which causes AIDS. At Antibody-ELISA.com you will find all the information related to
Elisa.
ELISA is a plate based assay technique which
is
used
for
detecting
as
and
quantifying
substances
such
peptides,
antibodies
and
conjugated
with an antibody reacts with
hormones.
An
proteins,
enzyme
colorless substrate to generate a colored
product. Such substrate is called chromogenic
substrate. A number of enzymes have been
used for ELISA sample preparation such as
alkaline phosphatase, horse radish peroxidase
and beta galactosidase.
Among the different types of ELISA, Sandwich ELISA protocol detects antigen. In this
technique, antibody is coated on the microtiter well. A sample containing antigen is added to the
well and allowed to react with the antibody attached to the well, forming antigen-antibody
complex. After the well is washed, a second
enzyme-linked antibody specific for a different
epitope on the antigen is added and allowed to
react with the bound antigen. Then after unbound
secondary antibody is removed by washing and
finally substrate is added to the plate which is
hydrolyzed by enzyme to form coloured products.
Sandwich ELISA has several advantages such as
high specificity since two antibodies are used the
antigen is specifically captured and detected. It is suitable for complex samples, since the
antigen does not require purification prior to measurement. It offers flexibility and sensitivity,
since both direct and indirect detection methods can be used. It is competitive ELISA as this
test is used to measure the concentration of an antigen in a sample.
About Antibody Elisa:
Modern medicine recognizes the complications of treating infectious diseases. It is realized
that early diagnosis of diseases is extremely important these days.
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