PEG / Tacsimate pH 5.8

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PEG / Tacsimate pH 5.8
PEG / Tacsimate pH 5.8
TM
Crystallization Reagent for Silver Bullets
TM
Solutions for Crystal Growth
User Guide
HR2-090
Description
• 1 milliliter of reagent filled in a 96 Deep Well block.
• Thermal sealed.
Crystallization Reagent refers to the solution that appears in both the reagent well (reservoir) and the crystallization drop. This reagent is sometimes
called precipitant, crystallant, dehydrant, or well solution.
Storage
Store between -20 and 4 degrees Celsius. May be stored at room temperature for
up to 30 days. For best results, allow block to equilibrate to 25 degrees Celsius
(room temperature) before removing seal. After use, seal the block using either
AlumaSeal II Sealing Film (HR8-069) or for best results, the block should be
sealed using a thermal sealer.
Related Products
HR2-096
Silver Bullets HT kit
HR2-088
Silver Bullets Bio HT kit
HR2-527
50% w/v Polyethylene glycol 3,350 200 ml
HR2-943-07 1.0 M MES monohydrate pH 5.8
185 ml
HR2-827
100% Tacsimate pH 6.0
200 ml
Application
PEG/Tacsimate pH 5.8 Crystallization Reagents for Silver Bullets is designed for
use as the crystallization reagent with the Silver Bullets kits. Refer to the Silver
Bullets kit User Guide for information about how the PEG/Tacsimate pH 5.8
Crystallization Reagent is used with the Silver Bullets.
Formulation
Reagent A1 - G12
25% w/v Polyethylene glycol 3,350,
0.1 M MES monohydrate pH 5.8
See Figure 1 ◊
0.25 ml, Deep Well Block
0.25 ml, Deep Well Block
HR8-069
AlumaSeal II Sealing Film
Pack of 100
Figure 1◊
1
3
4
6
7
9
10
12
A
Reagent H1 - H12
B
C
55% v/v Tacsimate pH 6.0,
0.1 M MES monohydrate pH 5.8
D
E
See Figure 1 ◊
F
G
H
◊ The reagent color featured in Figure 1 are for illustration purposes only.
Recommended Stock Solutions
Polyethylene glycol 3,350
• Synonyms: PEG 3,350
• Formula: H(OCH2CH2)nOH
• Formula Weight: 3,300 - 3,400
• CAS Number: [25322-68-3]
• EC Number: 500-038-2
• Merck: 13,7651
* 50% w/v Polyethylene glycol 3,350 (HR2-527) can
be used to reproduce HR2-090 reagents A1 - G12.
Tacsimate pH 6.0
A pH titrated mixture of organic acids.
• 1.8305 M Malonic acid
• 0.25 M Ammonium citrate tribasic
• 0.12 M Succinic acid
• 0.30 M DL-Malic acid
• 0.40 M Sodium acetate trihydrate
• 0.50 M Sodium formate
• 0.16 M Ammonium tartrate dibasic
• pH adjusted to 6.0 using NaOH
* 100% Tacsimate pH 6.0 (HR2-827) can be used to
reproduce HR2-090 reagents H1 - H12.
MES monohydrate pH 5.8
• Synonyms:
MES,
2-(N-morpholino)ethanesulfonic acid,
4-morpholineethanesulfonic acid monohydrate
• Formula: C6H13NO4S • H2O
• Formula Weight: 213.25
• CAS Number: [145224-94-8]
• EC Number: 224-632-3
* 1.0 M MES monohydrate pH 5.8 (HR2-943-07) can
be used to reproduce HR2-090 reagents A1 - H12.
Hampton Research
34 Journey
Aliso Viejo, CA 92656-3317 U.S.A.
Tel: (949) 425-1321 • Fax: (949) 425-1611
Technical Support e-mail: [email protected]
Website: www.hamptonresearch.com
HR2-090 - PEG/Tacsimate pH 5.8 User Guide - Lot 209004
hamptonresearch.com
Page 1 of 1
PEG / Tacsimate pH 6.8
TM
Crystallization Reagent for Silver Bullets
TM
Solutions for Crystal Growth
User Guide
HR2-092
Description
• 1 milliliter of reagent filled in a 96 Deep Well block.
• Thermal sealed.
Crystallization Reagent refers to the solution that appears in both the reagent
Storage
Store between -20 and 4 degrees Celsius. May be stored at room temperature for
up to 30 days. For best results, allow block to equilibrate to 25 degrees Celsius
(room temperature) before removing seal. After use, seal the block using either
AlumaSeal II Sealing Film (HR8-069) or for best results, the block should be
sealed using a thermal sealer.
Related Products
HR2-096
Silver Bullets HT kit
0.25 ml, Deep Well Block
HR2-088
Silver Bullets Bio HT kit
0.25 ml, Deep Well Block
HR2-527
50% w/v Polyethylene glycol 3,350
well (reservoir) and the crystallization drop. This reagent is sometimes called
precipitant, crystallant, dehydrant, or well solution.
HR2-931-01 1.0 M HEPES sodium pH 6.8
Application
PEG/Tacsimate pH 6.8 Crystallization Reagents for Silver Bullets is designed for
use as the crystallization reagent with the Silver Bullets kits. Refer to the Silver
Bullets kit User Guide for information about how the PEG/Tacsimate pH 6.8
Crystallization Reagent is used with the Silver Bullets.
Formulation
Reagent A1 - G12
25% w/v Polyethylene glycol 3,350,
0.1 M HEPES sodium pH 6.8
See Figure 1 ◊
200 ml
185 ml
HR2-755
100% Tacsimate pH 7.0
200 ml
HR8-069
AlumaSeal II Sealing Film
Pack of 100
Figure 1◊
1
3
4
6
7
9
10
12
A
Reagent H1 - H12
B
C
55% v/v Tacsimate pH 7.0,
0.1 M HEPES sodium pH 6.8
D
E
See Figure 1 ◊
F
G
H
◊ The reagent color featured in Figure 1 are for illustration purposes only.
Recommended Stock Solutions
Polyethylene glycol 3,350
• Synonyms: PEG 3,350
• Formula: H(OCH2CH2)nOH
• Formula Weight: 3,300 - 3,400
• CAS Number: [25322-68-3]
• EC Number: 500-038-2
• Merck: 13,7651
* 50% w/v Polyethylene glycol 3,350 (HR2-527) can
be used to reproduce HR2-092 reagents A1 - G12.
Tacsimate pH 7.0
A pH titrated mixture of organic acids.
• 1.8305 M Malonic acid
• 0.25 M Ammonium citrate tribasic
• 0.12 M Succinic acid
• 0.30 M DL-Malic acid
• 0.40 M Sodium acetate trihydrate
• 0.50 M Sodium formate
• 0.16 M Ammonium tartrate dibasic
• pH adjusted to 7.0 using NaOH
* 100% Tacsimate pH 7.0 (HR2-755) can be used to
reproduce HR2-092 reagents H1 - H12.
HEPES sodium pH 6.8
• Synonyms:
HEPES sodium salt,
4-(2-hydroxyethyl)piperazine-1-ethanesulfonic
acid sodium salt,
N-(2-hydroxyethyl)piperazine-N’-(2ethanesulfonic acid) sodium salt
• Formula: C8H17N2NaO4S
• Formula Weight: 260.29
• CAS Number: [75277-39-3]
• EC Number: 278-169-7
* 1.0 M HEPES sodium pH 6.8 (HR2-931-01) can be
used to reproduce HR2-092 reagents A1 - H12.
PEG / Tacsimate pH 7.8
TM
Crystallization Reagent for Silver Bullets
TM
Solutions for Crystal Growth
User Guide
HR2-094
Description
• 1 milliliter of reagent filled in a 96 Deep Well block.
• Thermal sealed.
Crystallization Reagent refers to the solution that appears in both the reagent
Storage
Store between -20 and 4 degrees Celsius. May be stored at room temperature for
up to 30 days. For best results, allow block to equilibrate to 25 degrees Celsius
(room temperature) before removing seal. After use, seal the block using either
AlumaSeal II Sealing Film (HR8-069) or for best results, the block should be
sealed using a thermal sealer.
Related Products
HR2-096
Silver Bullets HT kit
HR2-088
Silver Bullets Bio HT kit
0.25 ml, Deep Well Block
0.25 ml, Deep Well Block
HR2-527
50% w/v Polyethylene glycol 3,350 HR2-993-16 1.0 M BIS-TRIS propane pH 7.8 HR2-829
100% Tacsimate pH 8.0
200 ml
185 ml
200 ml
HR8-069
Pack of 100
well (reservoir) and the crystallization drop. This reagent is sometimes called
precipitant, crystallant, dehydrant, or well solution.
Application
PEG/Tacsimate pH 7.8 Crystallization Reagents for Silver Bullets is designed for
use as the crystallization reagent with the Silver Bullets kits. Refer to the Silver
Bullets kit User Guide for information about how the PEG/Tacsimate pH 7.8
Crystallization Reagent is used with the Silver Bullets.
Formulation
Reagent A1 - G12
25% w/v Polyethylene glycol 3,350,
0.1 M BIS-TRIS propane pH 7.8
See Figure 1 ◊
AlumaSeal II Sealing Film
Figure 1◊
1
3
4
6
7
9
10
12
A
Reagent H1 - H12
B
C
55% v/v Tacsimate pH 8.0,
0.1 M BIS-TRIS propane pH 7.8
D
E
F
See Figure 1 ◊
G
H
◊ The reagent color featured in Figure 1 are for illustration purposes only.
Recommended Stock Solutions
Polyethylene glycol 3,350
• Synonyms: PEG 3,350
• Formula: H(OCH2CH2)nOH
• Formula Weight: 3,300 - 3,400
• CAS Number: [25322-68-3]
• EC Number: 500-038-2
• Merck: 13,7651
* 50% w/v Polyethylene glycol 3,350 (HR2-527) can
be used to reproduce HR2-094 reagents A1 - G12.
Tacsimate pH 8.0
A pH titrated mixture of organic acids.
• 1.8305 M Malonic acid
• 0.25 M Ammonium citrate tribasic
• 0.12 M Succinic acid
• 0.30 M DL-Malic acid
• 0.40 M Sodium acetate trihydrate
• 0.50 M Sodium formate
• 0.16 M Ammonium tartrate dibasic
• pH adjusted to 8.0 using NaOH
* 100% Tacsimate pH 8.0 (HR2-829) can be used to
reproduce HR2-094 reagents H1 - H12.
BIS-TRIS propane pH 7.8
• Synonyms:
•1,3-Bis[tris(hydroxymethyl)methylamino]propane
• pH adjusted to 7.8 using HCl
• Formula: C11H26N2O6
• Formula Weight: 282.34
• CAS Number: [64431-96-5]
• EC Number: 264-899-3
• Beilstein Registry Number: 1786109
* 1.0 M BIS-TRIS propane pH 7.8 (HR2-993-16) can
be used to reproduce HR2-094 reagents A1 - H12.
Hampton Research
34 Journey
Aliso Viejo, CA 92656-3317 U.S.A.
Tel: (949) 425-1321 • Fax: (949) 425-1611
Technical Support e-mail: [email protected]
Website: www.hamptonresearch.com
HR2-094 - PEG/Tacsimate pH 7.8 User Guide - Lot 209404
hamptonresearch.com
Page 1 of 1

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