alone or HB-EGF and actinomycin D

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alone or HB-EGF and actinomycin D
Heparin-Binding Epidermal Growth Factor-Like Growth
Factor Regulates Fibroblast Growth Factor-2 Expression in
Aortic Smooth Muscle Cells
by Kimberly A. Peifley, Gregory F. Alberts, Debbie K.W. Hsu, Sheau-Line Y. Feng,
and Jeffrey A. Winkles
Circulation Research
Volume 79(2):263-270
August 1, 1996
Copyright © American Heart Association, Inc. All rights reserved.
Effect of HB-EGF treatment on FGF-1 and FGF-2 mRNA levels in SMCs. A, Serum-starved SMCs
were either left untreated (NT) or treated with HB-EGF for 4 hours.
Kimberly A. Peifley et al. Circ Res. 1996;79:263-270
Copyright © American Heart Association, Inc. All rights reserved.
Effect of HB-EGF treatment on FGF-2 mRNA levels in SMCs: time-course and dose-response
analyses.
Kimberly A. Peifley et al. Circ Res. 1996;79:263-270
Copyright © American Heart Association, Inc. All rights reserved.
Effect of actinomycin D or cycloheximide on HB-EGF–induced FGF-2 mRNA expression in
SMCs. Serum-starved SMCs were either left untreated (NT) or treated for 4 hours with HB-EGF
alone, HB-EGF and actinomycin D (HB-EGF+Act.D), actinomycin D alone (Act.D), HB-EGF and
cycloheximide (HB-EGF+Chx), or cycloheximide alone (Chx).
Kimberly A. Peifley et al. Circ Res. 1996;79:263-270
Copyright © American Heart Association, Inc. All rights reserved.
Effect of HB-EGF treatment on FGF-2 mRNA stability in SMCs. Serum-starved SMCs were either
left untreated or treated with actinomycin D (Act.D) alone or HB-EGF and actinomycin D (HBEGF+Act.D).
Kimberly A. Peifley et al. Circ Res. 1996;79:263-270
Copyright © American Heart Association, Inc. All rights reserved.
Effect of dexamethasone on HB-EGF–induced FGF-2 mRNA expression in SMCs. Serum-starved
SMCs were either left untreated (NT) or treated for 4 hours with either HB-EGF alone or HB-EGF
in combination with 1, 10, or 100 nmol/L dexamethasone (Dex/1, Dex/10, and Dex/100,
respectively).
Kimberly A. Peifley et al. Circ Res. 1996;79:263-270
Copyright © American Heart Association, Inc. All rights reserved.
Effect of heparin on HB-EGF–induced or EGF-induced FGF-2 mRNA expression in SMCs. Serumstarved SMCs were either left untreated (NT) or treated for 4 hours with HB-EGF or EGF alone or
in combination with 10, 30, or 100 μg/mL heparin (Hep/10, Hep/30, and Hep/100, respectively).
Kimberly A. Peifley et al. Circ Res. 1996;79:263-270
Copyright © American Heart Association, Inc. All rights reserved.
Expression of FGF-2 protein in HB-EGF–treated SMCs. A, Equivalent amounts of recombinant
FGF-1 or FGF-2 (18-kD isoform) were subjected to SDS-PAGE, transferred to a nitrocellulose
membrane, and incubated with an anti–FGF-2 antibody.
Kimberly A. Peifley et al. Circ Res. 1996;79:263-270
Copyright © American Heart Association, Inc. All rights reserved.

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