Department of Medical Biochemistry

DEPARTMENT OF MEDICAL BIOCHEMISTRY
Address: Maydan Voli str., 1, Тernopil, 46001
Tel: (0352) 254784
Department staff: 2 Professors, 4 Associate Professors, 2
senior instructors, 4 Assistant Professors, 4 laboratory assistants
Head of the Department – Prof. Mykhaylo Korda, MD, PhD, DSc.
Biochemistry Department was founded in 1957. Prof. Zenta Gude was the first
Head of the Department (1958-1982). In 1982-1985 the Department was headed by
Assist. Prof. Agnia Kyyashko and then Prof. Yaroslav Honskiy was the Head of the
Department from 1985 till 2006 р. From 2007 Medical Biochemistry Department is
headed by Prof. Mykhaylo Korda.
The Department offers an exciting and challenging environment for medical
students.
Courses that are taught at the Department:
Biochemistry for the students of Medical Faculty (2nd year)
Biochemistry for the students of Dentistry Faculty (2nd year)
Biochemistry for the students of Pharmaceutical Faculty (3rd year)
Biochemistry for the students of Nursing School
Professor M. Korda conducts seminar 'Biochemistry of immune processes' for foreign
students of Medical Faculty
During the practical class at the Department
Professor Ya. Honskiy conducts the seminar 'General principles of the regulation of
metabolic processes in human organism' for the students of Dentistry Faculty
Associate Professor O. Ostrivka explains the new material to foreign students of
Medical Faculty
Associate Professor S. Pidruchna controls the results of on-line students’ test
Associate Professor S. Yavorska during the seminar with foreign students of Medical
Faculty
Associate Professor G. Shershun is checking the student’s protocol of practical class
Associate Professor N. Sayuk with the students of Dentistry Faculty during the
practical class 'Biological oxidation. Metabolism of energy'
Assistant Professor I. Kuzmak explains student the peculiarities of practical work
Scientific work at the Department
The research interests of the Department staff are in the area of free radical
biochemistry. Particular interests are centered in the three following fields:
1) Free radical hepatotoxicology. We are interested in the mechanisms of
reactive oxidant and nitrogen production in hepatocytes, the biochemistry of
biological damage and the consequences of oxidative and nitrosooxidative stress as
well as the mechanisms that liver cells use to attenuate free radical damage. Particular
interest is centered on the interaction of cytochrome P450 dependent monooxigenase
system of microsomes and reactive nitric oxide species and its role in cell damage by
xenobiotics.
2) Studying of the role of nitric oxide, peroxynitrate and superoxide in the
cardiovascular system. Research focuses on the understanding of the endothelial
dysfunction mechanisms in obesity, hypertension, and atherosclerosis. We are
especially interested in the elucidating of the role of adipose tissue derived hormone
leptin in the vascular tone control. We discovered that chronic hyperleptinemia
accompanying obesity can modify the endothelial NO, О2•, ОNOO– – metabolism and
cause endothelial dysfunction.
3) Free radical mechanisms of atherosclerosis development. Our research is
focused on the mechanisms of low density lipoproteins (LDL) modification and
prevention of such modification by antioxidants. We study the relationship between
atherosclerosis pathogenesis and saturation of LDL with natural antioxidants
(tocopherols, carotenoids, phlavonoids). We also proposed several new drugs that are
able to inhibit LDL oxidation and therefore have potential antiatherogenic properties.
Selected publications
1. Korda M.M., Yaroshenko T.Ya., Samborska S.M., Korda I.V. The effect of leptin on the expression of NO-synthase
in endothelial cells. Medical Chemistry. 2009;11(1):135-138 (In Ukrainian).
2. Korda M.M., Yaroshenko T.Ya., Samborska S.M., Korda I.V. The effect of leptin on the NO, O2 – і ONOOformation in endothelial cells. Medical Chemistry. 2009;11(3):13-17 (In Ukrainian).
3. Korda M, Pokotylo O, Yaroshenko T, Yavorska S, Ostrivka O. Effect of ?-blockers on aging endothelium. Ann.
Univers. Mariae Curie-Sklodowska. 2008;21(2):281-284.
4. Korda M, Kubant R, Patton S, Malinski T. Leptin-induced endothelial dysfunction in obesity. Am J Physiol Heart
Circ Physiol. 2008;295(4):H1514-21.
5. Funovic P, Korda M, Kubant R, Barlag RE, Jacob RF, Mason RP, Malinski T. Effect of beta-Blockers on Endothelial
Function During Biological Aging: A Nanotechnological Approach. J. Cardiovasc. Pharmacol. 2008;51(2):208-215.
6. Yaroshenko T., Korda M. Correction of the allyl alcohol liver injury by the iNOS inhibitor 1400W and NOS
substrate L-arginine combination. Med. Chem. 2007;9(4):24–27 (In Ukrainian).
7. Yaroshenko T., Korda M. Role of nitric oxide in chemically induced hepatotoxicity. Ann. Univers. Mariae CurieSklodowska. 2006;19(3):143-146.
8. Korda M., Yaroshenko T. Role of nitric oxide in the pathogenesis of liver damage by xenobiotics. Med. Chem.
2005;7(3):74–80 (In Ukrainian).
9. Madajka M, Korda M, White J. Malinski T. Effect of aspirin on constitutive nitric oxide synthase (cNOS) and the
biovailability of NO // FASEB J. – 2004. - 18 (5): A1319-A1319 Suppl. S. – Mar. 24, 2004.
10. Korda M., Yaroshenko T. The effect of iNOS inhibitor N-(3-(Aminomethyl)benzyl)acetamidine on the allyl alcohol
hepatotoxicity. Med. Chem. 2004;6(3):114–116 (In Ukrainian).
11. Korda M., Liu F. Age related structural and functional disorders of mitochondrial membranes of hepatocytes in a
toxic hepatitis. Europ. J. Biochem. 2004;271, Suppl.1:176.
12. Madajka M., Korda M., White J., Malinski T. Effect of aspirin on constitutive nitric oxide synthase and the
bioavailability of NO. Thromb Res. 2003;110:317-321.
13. Korda M. Protection of hepatocytes from galactosamine toxicity by cresacin. Free Radic Biol Med. 2002; 33 (Suppl.
1):605.
14. Korda M.M. Lipid peroxidation is a prerequisite for galactosamine-induced damage. Curr. Topics in Biophys.
2000;24(2):83-87.
15. Korda M.M. Age-related changes in liver plasma membrane lipid composition and phospholipase A2 activity of rats
with D-galactosamine hepatitis. Chem. and Phys. of Lipids. 2000;107(1):52.
16. Korda M. Mitochondrial respiratory chain disorders in galactosamine intoxication. Europ. Biophys. Journal.
2000;29(4):367.
17. Korda M. Oxidized LDL and vitamin E level in lipoproteins of patients with coronary heart disease. Biochem. Med.
2000;10(1-2):70.
18. Korda M. Inhibition of low density lipoproteins (LDL) oxidation by cresacin. Atherosclerosis. 1999;147(Suppl.
2):19.
19. Korda M.M. Cresacin inhibits oxidation of low density lipoproteins in vitro. Pathophysiology. 1998;5(Suppl. 1):47.
20. Korda M., Yaroshenko T. The role of nitric oxide in the pathogenesis of liver damage by xenobiotics. Med. Chem.
2005;7(4):85-91(In Ukrainian).
21. Korda M., Yaroshenko T. The effect of iNOS inhibitor N-(3-(Aminomethyl)benzyl)acetamidine on the allyl alcohol
hepatotoxicity. Med. Chem. 2004;6(3):114-117 (In Ukrainian).
22. Korda M., Malinski T. Leptin increases NO bioavailability in human vascular endothelial cells via
phosphatidylinositol 3-kinase-independent pathway. Cardiovascular Pathology. 2004;13(3):148-149
23. Klishch I.M., Korda M.M., Posokhova K.A., Shkrobot S.I. Age peculiarities of lipid status of rat liver at toxic
damage by tetrachlormethane. Med. Chem. 2003;5(1):44-48 (In Ukrainian).
24. Klishch I.M., Korda M.M. The using of enterosorbents and liposomes for the correction of oxidative processes in
mitochondria of different age rats with toxic liver injury. Med. Chem. 2002;4(4):36-39 (In Ukrainian).
25. Klishch I.M., Honsky Ya.I., Korda M.M. The effect of hydrazine chloride on the activity of NADPH- and
ascorbatdependent lipid peroxidation in microsomes of rats of different age. Med. Chem. 2002;4(2):15-18 (In
Ukrainian).
26. Pokotylo O.S., Korda M.M. Synthesis of lipids in animal muscles in different physiological states. Achiev. of Clin.
and Experim. Med. 2002;7:136-137 (In Ukrainian).
27. Mysula I.R., Bodnar Ya., Ya., Korda M.M. The effect of Salmonella endotoxin on the oxidative metabolism of
arachidonic acid, hemostasis and fibrinolysis. Bukovinian Med. J. 2002;1:154-157 (In Ukrainian).
28. Klishch I.M., Korda M.M. The effect of hydrazine chloride on the energetic processes in isolated hepatocytes. Med.
Chem. 2002;4(3):57-59 (In Ukrainian).
29. Honsky Ya.I., Kubant R.M., Korda M.M. Correction of oxidative and energetic processes in rats with toxic hepatitis
by unithiol. Med. Chem. 2002;4(1);46-49 (In Ukrainian). 30. Klishch I.M., Korda M.M. The degree of oxidative
modification of proteins and state of antioxidant system in rats with tetrachlormethane hepatitis. Data Sci. Res.
2002;1;39-42 (In Ukrainian).
31. Klishch I.M., Korda M.M., Boychuk A.V. Using of phosphatydilcholine liposomes for correction of functional state
disorders of liver mitochondria in rats with tetrachlormethane hepatitis. Med. Chem. 2002; 4(1):17-20 (In Ukrainian).
32. Klishch I.M., Korda M.M., Fira L.S. The efficiency of ceolite for the correction of microsomal oxidation disorders
in animals with toxic liver injury by tetraclormethane // Data Sci. Res. 2001;3:114-116 (In Ukrainian).
33. Honsky Ya.I., Klishch I.M., Korda M.M. The effect of carnithine chloride on the energetic processes in liver of
different age animals in hydrazine intoxication. Med. Chem. 2001;3(3):29-35 (In Ukrainian).
34. Fira L.S., Honsky Ya.I., Korda M.M. The disorders of metabolic processes in animals with experimental toxicosis
after X-ray irradiation. Achiev. of Clin. and Experim. Med. 2001;6: 16-19 (In Ukrainian).
35. Korda M.M. The effect of electromagnetic waves of millimeter length on the dynamics of energy formation in rat
heart mitochondria in food deprivation and adrenal myocardial dystrophy. Оdessa Med. J. 2001;1(63):11-13 (In
Ukrainian).
36. Hnatyuk M.S., Slyvka Yu.I., Korda M.M. Morphometric evaluation of structural rearrangement of heart in
epinephrine myocardiodystrophia and starvation. Оdessa Med. J. 2000;6(62):5-7 (In Ukrainian).
37. Korda I.V., Malanchuk L.M., Korda M.M. The prophylaxis of the violations of oxidative processes in women after
caesarian section operation by fluenizid and zinacef. Med. Chem. 2000;2(3):37-40 (In Ukrainian).
38. Korda M.M., Petrukh L.I., Korda I.V. The studying of antioxidative properties of flurenizid. Achiev. of Clin. and
Experim. Med. 2000;5:502-505 (In Ukrainian).
39. Honsky Ya.I., Fira L.S., Korda M.M. Correction of the endogenic intoxication in nitrite poisoning by trecresan.
Achiev. of Clin. and Experim. Med. 2000;5:479-482 (In Ukrainian).
40. Korda M.M., Petrukh L.I. Antiradical and antioxidant activity of N-(9-fluoreniliden)-N’-isonicotinohydrazide
(flurenizid). Med. Chem. 2000;2(2):15-18 (In Ukrainian).
41. Yepishyn A.V., Babyak N.I., Korda M.M. The effect of isoprinozine on the level of lipoperoxidation and
antioxidant status in patients with chronic bronchitis combined with HBV-infection. Med. Chem. 1999;1(1):90 – 92 (In
Ukrainian).
42. Honsky Ya.I., Korda M.M., Saiuk N.P. Effect of vitamins A, E, C combination on mitochondrial oxidation in rats
different age with tetrachlormethane hepatitis. Achiev. of Clin. and Experim. Med. 1999;4:389-392 (In Ukrainian).
43. Korda M.M., Shershun G.G., Saiuk N.P. Effect of galactosamine on the respiratory activity of isolated hepatocytes.
Achiev. of Clin. and Experim. Med. 1999;3:388-391 (In Ukrainian).
44. Korda M.M. Hepatoprotective properties of cresacin in toxic action of galactosamine. Drugs. 1998;1:44 – 46 (In
Ukrainian).
45. Korda M.M. Protective effect of cresacin in acute experimental hepatitis induced by D-galactosamine. Ukr.
Biochem. J. 1998;1:69 – 75 (In Ukrainian).
46. Korda M.M. Tris(2-oxyethyl)ammonium orthocrezoxyacetate inhibits the oxidative modification of low density
lipoproteins. Experim. and Clin. Pharmacol. 1998;6:37 – 39 (In Russian).
47. Gnatiuk M.S., Kuziv P.P., Slyvka Y.I., Korda M.M. Effect of food deprivation on the experimental adrenaline
myocardiodystrophy. Data of Scient. Res. 1998;6:30-31(In Ukrainian).
48. Korda M.M., Brodin S.V., Stravsky Ia.S. Using of liposomes in clinical medicine. Drugs. 1997;5:67-72 (In
Ukrainian).
49. Korda M.M., Brodin S.V., Honsky Ya.I. Correction of metabolic disorders in liver with D-galactosamine
intoxication by phosphatidylcholine liposomes and liposomes with incorporated cresacin. – Drugs. 1997;3:24-27 (In
Ukrainian).
50. Korda M.M., Honsky Ja.I., Fira L.S. Isolated hepatocytes: using in experimental and clinical hepatology. J.
Academy of Med. Sci. of Ukraine. 1997;1:33 – 44 (In Ukrainian).
51. Korda M.M. Reparation of liver mitochondrial membranes injured by galactosamine with the help of liposomes.
Data of Biol. and Med. Problems. 1997;11:117–119 (In Ukrainian).
52. Korda M.M. The protective effect of phosphatydylcholine liposomes in experimental galactosamine hepatitis. Data
of Biol. and Med. Problems. 1997;11:114–116 (In Ukrainian).
53. Korda M.M., Fira L.S., Shershun H.H. Metabolism of amino acid [U-14C]leucine in experimental galactosamine
hepatitis. Achiev. of Clin. and Experim. Med. 1997;2:550-553 (In Ukrainian).
54. Korda M.M. The studying of antioxidant properties of tris(2-oxyethyl)ammonium orthocrezoxyacetate. Pharmac. J.
1996;5-6:92-93 (In Ukrainian).
55. Honsky Ya.I., Korda M.M., Klishch I.M., Fira L.S. Correction of oxidative processes disorders in chemical liver
injury by dimethylsulfoxide and tocopherol. Probl. of Med. Chem. 1996;42(1):30-34 (In Russian).
56. Honsky Ya.I., Korda M.M., Klishch I.M., Fira L.S. Role of antioxidant system in pathogenesis of toxic hepatitis.
Pathol. Physiol. and Experim. Ther. 1996;2:43-45.
57. Korda M.M. Status of antioxidant, monooxigenase and humoral immune systems in D-galactosamine hepatitis. Ukr.
Biochem. J. 1996;1:72-75 (In Ukrainian).
58. Honsky Ya.I., Korda M.M., Klishch I.M., Fira L.S. Correction of oxidative processes in toxic liver injury by
acetylcysteine. Experim. and Clin Pharmacol. 1995;6:64-66 (In Russian).
59. Honsky Ya.I., Korda M.M., Klishch I.M., Fira L.S. Antioxidative properties of acetylcysteine. Pharmac. J.
1995;5:75-76 (In Russian).
60. Honsky Ya.I., Bahan O.P., Korda M.M. Correction of oxidative processes in toxic liver injury by enterosorption.
Ukr. Biochem. J. 1994;2:112–116 (In Ukrainian).
61. Honsky Ya.I., Korda M.M., Klishch I.M. Antioxidant effect of dimethylsulfoxide in acute liver tetrachlormethane
injury. Probl. of Med. Chem. 1992;2:43-44 (In Russian).
62. Bekh M.D., Basistiuk I.I., Korda M.M. Lipid peroxidation and impairement in erythrocytic properties in patients
with ulcer disease before and after surgery. Clin. Surg. 1992;(11):23-25 (In Russian).
63. Gonskii Ia.I., Korda M.M., Klishch I.M. The effect of acetylcysteine on the antioxidant system in an experimental
toxic lesion of the liver. Farmakol. Toksikol. 1991;54(5):44-46 (In Russian).
64. Gons'kyi Ia.I., Korda M.M., Klishch I.M.. Status of the free radical oxidation and antioxidant system in rats with
toxic liver damage; effect of tocopherol and dimethylsulfoxide. Ukr Biochem. J. 1991;63(5):112-116 (In Ukrainian).
65. Bekh N.D., Basistiuk I.I., Korda M.M., Martyniuk A.E.. Erythrocyte function in patients with complicated peptic
ulcer. Vrach Delo. 1989;10:25-28 (In Russian).