4 Spring 2015

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4 Spring 2015
Syllabus
MCB 301
Laboratory Schedule
Date (pt total)
Experiment
DAY 1
Course introduction
Wed Jan 21
1: Streak plate
Th
2: Luminescent bacteria
10-13
DAY 2
1, 2: Evaluations
8, 13
Mon Jan 26
3: Spread plate
14-17
Tu
4: Simple stain
18-29
DAY 3
3: Evaluations
17
Wed Jan 28
5: Gram stain
30-32
DAY 4 (12)
6: Wet mount, Darkfield and Phase Contrast
33-42
Mon Feb 2
7a-d: Enzymes and adaptation, 1
43-54
DAY 5 (33)
7a-d: Enzymes and adaptation, 2
43-54
Wed Feb 4
8a: Lactic acid bacteria enrichment
55-63
8b: Lactic acid bacteria isolation
63-64
DAY 7 (42)
8c: Lactic acid bacteria observation
64-65
Wed Feb 11
9a-b: Environmental effects, 1
66-75
DAY 8 (20)
9a-b: Environmental effects, 2
72-75
Mon Feb 16
9c-e: Environmental effects, 1
76-81
DAY 9 (32)
9c-e: Environmental effects, 2
76-81
Wed Feb 18
10a: Sporeformers enrichment
82-93
Th
Tu
Th
Jan 22
Jan 27
Lab Manual Pages
I-XXXII
1-9
Jan 29
Feb 3
Feb 5
DAY 6 (22)
Mon Feb 9
Tu Feb 10
Th
Feb 12
Tu Feb 17
Th
Feb 19
II
Syllabus
MCB 301
Date (pt total)
Experiment
Lab Manual Pages
DAY 10 (12)
10b: Sporeformers isolation, 1
94-95
10b: Sporeformers isolation, 2
96-97
Mon Feb 23
Tu Feb 24
DAY 11 (13)
Wed Feb 25
Th
Feb 26
DAY 12 (13)
10c: Sporeformers post-enrichment, 1
98-99
Mon Mar 2
Tu
Mar 3
DAY 13 (25)
10c: Sporeformers post-enrichment, 2
99-102
Wed Mar 4
12a: Transposon mutagenesis conjugation
114-118
12b: Transposon mutagenesis selection, 1
119
DAY 15 (34)
12b: Transposon mutagenesis selection, 2
120
Wed Mar 11
13a-c: Biochemical tests, 1
127-158
DAY 16 (34)
12c: Transposon mutagenesis characterization, 1
121-124
Mon Mar 16
13a-c: Biochemical tests, 2
Th
Mar 5
DAY 14 (12)
Mon Mar 9
Tu
Th
Tu
Mar 10
Mar 12
127-159
Mar 17
DAY 17 (13)
12c: Transposon mutagenesis characterization, 2
Wed Mar 18
Th
Mar 19
Sat Mar 21 to
Spring Break
Sun Mar 29
III
121-126
Syllabus
MCB 301
Date (pt total)
Experiment
Lab Manual Pages
DAY 18 (18)
14a: Identification isolation
160-168
Mon Mar 30
15a: Southern blot electrophoresis
174-188
DAY 19 (19)
14b: Identification purification, 1
169-170
Wed Apr 1
15b: Southern blot transfer
189-195
Tu Mar 31
Th
Apr 2
Th Apr 3
OPEN HOURS BEGIN, SEE SCHEDULE
DAY 20 (18)
14b: Identification purification, 2
169-170
Mon Apr 6
15c: Southern blot hybridization
196-201
DAY 21 (19)
14c: Identification dichotomous keys; inoculate
171-172
Wed Apr 8
tests, 1
202-203
Th
15d: Southern blot washing
Tu Apr 7
Apr 9
DAY 22 (19)
14c: Identification; inoculate tests, 2
171-172
Mon Apr 13
15e: Southern blot detection
204-207
DAY 23 (19)
14c: Identification; inoculate/score tests, 3
171-173
Wed Apr 15
15f: Southern blot analysis
208-209
Tu Apr 14
Th
Apr 16
Fri Apr 17
OPEN HOURS END
DAY 24 (25)
16a: ELISA fluid exchange
210-218
Mon Apr 20
17a-b: MIC determination, 1
224-230
DAY 25 (25)
16b: ELISA analysis
218-223
Wed Apr 22
17a-b: MIC determination, 2
228-231
Tu Apr 21
Th
Apr 23
IV
Syllabus
MCB 301
Date (pt total)
Experiment
Lab Manual Pages
DAY 26
Lab Practical Review and Practice
Mon Apr 27
Lab clean up & drawer inventory
Tu Apr 28
DAY 27 (42)
Wed Apr 29
Th
Apr 30
DAY 28 (58)
Mon May 4
Tu May 5
Lab Practical Exam, Part I
Dilution problem calculation, Gram stain, brightfield
microscopy, streak plate
Lab Practical Exam, Part II
Dilutions & spread plate, wet mount & phase
contrast, biochemical tests, dichotomous key
V
Syllabus
MCB 301
Lecture Schedule
Date
Topic(s)
January 23
Lecture 1
Basic microbiology techniques (aseptic technique, pure culture, plating);
Luminescent bacteria; Microscopy; Staining (cell wall structure)
January 30
Lecture 2
Microscopy; Enzymes and adaptation
February 6
Lecture 3
Lactic acid bacteria
February 13
Exam 1
Basic microbiology techniques; Microscopy; Staining; Luminescent
bacteria; Enzymes and adaptation
February 20
Lecture 4
Bacterial Growth, Environmental effects
February 27
Lecture 5
Environmental effects; Sporeformers
March 6
Exam 2
Lactic acid bacteria; Bacterial growth; Environmental effects;
Biochemical tests
March 13
Lecture 6
DROP DEADLINE
March 20
Lecture 7
Transposon mutagenesis
March 27
Spring Break
April 3
Lecture 8
Identification; Southern blot: DNA digestion and electrophoresis, transfer,
hybridization
April 10
Exam 3
Sporeformers; Transposon mutagenesis; Biochemical tests
April 17
Southern blot: probe synthesis, washing, detection, analysis and ELISA
Lecture 9
April 24
Lecture 10
Agglutination; MIC; Dilution problem
May 1
Lecture 11
Restriction mapping; Southern blot analysis problem
Final Exam
FINALS WEEK
Identification; ELISA/agglutination; Southern blot; MIC; Restriction
mapping; Southern blot analysis problem
VI
Syllabus
MCB 301
Laboratory Assignment Schedule
Date
Assignment Due
DAY 1
Wed Jan 21
Th Jan 22
Nothing Due
DAY 2
Mon Jan 26
Tu Jan 27
Nothing Due
DAY 3
Wed Jan 28
Th Jan 29
Pre-lab 1-6: Basic Technique
10 points
DAY 4
Mon Feb 2
Tu Feb 3
Pre-lab 7: Enzymes and adaptation
10 points
DAY 5
Wed Feb 4
Th Feb 5
Pre-lab 8: Lactic acid bacteria
10 points
Post-lab 1-6: Basic Technique
10 points
DAY 6
Mon Feb 9
Tu Feb 10
Post-lab 7: Enzymes and adaptation
10 points
DAY 7
Wed Feb 11
Th Feb 12
7: Enzymes & adaptation Lab Report
24 points
Pre-lab 9: Environmental effects
10 points
Fri Feb 13
Point Total
Exam 1
Basic microbiology techniques; Microscopy;
Staining; Luminescent bacteria; Enzymes and
adaptation
50 points
DAY 8
Mon Feb 16
Tu Feb 17
Post-lab 8: Lactic acid bacteria
10 points
DAY 9
Wed Feb 18
Th Feb 19
Pre-lab 10: Sporeforming bacteria
10 points
8: Lactic acid bacteria lab report
65 points
Fri Feb 20
11: Growth Curve opens in LON-CAPA at 2pm
DAY 10
Mon Feb 23
Tu Feb 24
Post-lab 9: Environmental effects
VII
10 points
Syllabus
MCB 301
Date
Assignment Due
DAY 11
Wed Feb 25
Th Feb 26
9: Environmental effects lab report
DAY 12
Mon Mar 2
Tu Mar 3
Nothing Due
DAY 13
Wed Mar 4
Th Mar 5
11: Growth Curve exercise on LON-CAPA
41 points
Pre-lab 12: Transposon mutagenesis
10 points
Fri Mar 6
Point Total
Exam 2
Lactic acid bacteria; Bacterial growth;
Environmental effects;
60 points
50 points
DAY 14
Mon Mar 9
Tu Mar 10
Post-lab 10: Sporeforming bacteria
10 points
DAY 15
Wed Mar 11
Th Mar 12
10: Sporeforming bacteria lab report
63 points
Pre-lab 13: Biochemical Tests
10 points
DAY 16
Mon Mar 16
Tu Mar 17
Nothing Due
DAY 17
Wed Mar 18
Th Mar 19
Post-lab 13: Biochemical tests
Sat Mar 21 to
Sun Mar 29
Spring Break
DAY 18
Mon Mar 30
Tu Mar 31
Post-lab 12: Transposon mutagenesis
10 points
13: Biochemical Tests Lab Report
43 points
Pre-lab 14: Identification
10 points
Pre-lab 15: Southern Blot
10 points
DAY 19
Wed Apr 1
Th Apr 2
12: Transposon mutagenesis Lab Report
62 points
DAY 20
Mon Apr 6
Tu Apr 7
Nothing Due
10 points
10 points
VIII
Syllabus
MCB 301
Date
Assignment Due
DAY 21
Wed Apr 8
Th Apr 9
Nothing Due
Point Total
Fri Apr 10
Exam 3
50 points
Sporeformers; Transposon mutagenesis;
Biochemical Tests
DAY 22
Mon Apr 13
Tu Apr 14
Nothing Due
DAY 23
Wed Apr 15
Th Apr 16
Nothing Due
DAY 24
Mon Apr 20
Tu Apr 21
Post-lab 14: Identification
10 points
Pre-lab 16: ELISA
10 points
Pre-lab 17: MIC determination
10 points
DAY 25
Wed Apr 22
Th Apr 23
14: Identification Lab Report
47 points
Post-lab 15: Southern Blot
10 points
DAY 26
Mon Apr 27
Tu Apr 28
15: Southern Blot Lab Report (NO LATE LAB
REPORTS ACCEPTED)
65 points
Post-lab 16: ELISA
10 points
10 points
Post-lab 17: MIC
DAY 27
Wed Apr 29
Th Apr 30
16: ELISA Lab Report
26 points
17: MIC Lab Report
24 points
Lab Practical Exam, Part I
Dilution problem calculation, Gram stain,
brightfield microscopy, streak plate
DAY 28
Mon May 4
Tu May 5
Finals Week
Parts 1 and 2 worth a
total of 100 points
Lab Practical Exam, Part II
Dilutions & spread plate, wet mount & phase
contrast, biochemical tests, dichotomous key
Final Exam
Identification; ELISA/agglutination; Southern
blot; MIC; Restriction mapping; Southern blot
analysis
IX
50 points

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