Supplemental Data

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Supplemental Data
Supplementary Files:
A
HindIII
P35S
HPT
LB
XbaI
P54/1.0
P35S
P 35S
MeCu/ZnSOD
T35S
MeCAT1
Tnos
uidA
Tnos RB
Tnos
SC lines
B
(kb)
M
P 23 22 21 16 15 11
8
4
2 WT
23.1
9.4
6.6
4.3
2.3
HPT Probe
2.0
23.1
gCAT
9.4
6.6
4.3
gCAT
2.3
2.0
Figure
S1.
MeCAT1 Probe
A.
Schematic
presentation
of
the
T-DNA
region
of
pC-P54::MeCu/ZnSOD-35S::MeCAT1. LB, left border; RB, right border of T-DNA; T35S,
CaMV 35S terminator; TNOS, NOS terminator ; P35S, cauliflower mosaic virus 35S promoter;
P54/1.0, vascular-specific promoter p54/1.0; HPT, hygromycin phosphotransferase. B. Southern
blot analysis of transgenic cassava lines using DIG-labeled HPT and MeCAT1 probes. 20 μg of
total DNA was digested with XbaI, separated by agarose gel electrophoresis, and subjected to
Southern blot analysis. gCAT, genomic DNA of cassava catalases; M, λ HindIII DIG-labeled
molecular marker; P, plasmid pC-P54::MeCu/ZnSOD-35S::MeCAT1; WT, wild-type control.
Numbers indicate different transgenic lines.
1/1

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