Cells-to-CT Kits - Thermo Fisher Scientific

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Cells-to-CT Kits - Thermo Fisher Scientific
Ordering information
Cells-to-CT kits
Each kit contains sufficient reagents to perform five qRT-PCR reactions per cell lysate. For example, a 100-reaction kit contains enough
reagents to prepare 100 cell lysates and perform 500 qRT-PCR reactions.
Product
Quantity
Cat. No.
20 reactions
A25605
100 reactions
A25603
400 reactions
A25602
40 reactions
4399002
100 reactions
AM1728
400 reactions
AM1729
TaqMan MicroRNA Cells-to-CT Kit
100 reactions
4391848
TaqMan Fast Cells-to-CT Kit
100 reactions
4399003
20 reactions
A25601
100 reactions
A25600
400 reactions
A25599
40 reactions
4402953
100 reactions
4402954
400 reactions
4402955
100 reactions
4402956
400 reactions
4402957
50 reactions
4458237
400 reactions
4458236
Cells-to-CT Stop Solution
1 mL
4402960
Cells-to-CT Bulk Lysis Reagents
2,500 reactions
4391851C
Stabilized Blood-to-CT Nucleic Acid Preparation Kit for qPCR,
compatible with PAXgene or Tempus Blood RNA Tubes
50 reactions
4449079
Stabilized Blood-to-CT Nucleic Acid Preparation Kit for qPCR,
compatible with PAXgene Blood RNA Tubes
200 reactions
4449082
Stabilized Blood-to-CT Nucleic Acid Preparation Kit for qPCR,
compatible with Tempus Blood RNA Tubes
200 reactions
4449080
SYBR Green Cells-to-CT Control Kit
100 reactions
4402959
Cells-to-CT 1-Step TaqMan Kit
TaqMan Gene Expression Cells-to-CT Kit
Cells-to-CT 1-Step Power SYBR Green Kit
Power SYBR Green Cells-to-CT Kit
Fast SYBR Green Cells-to-CT Kit
Single Cell-to-CT qRT-PCR Kit
Cells-to-C T Kits
Find out more at thermofisher.com/cellstoct
For Research Use Only. Not for use in diagnostic procedures. © 2015 Thermo Fisher Scientific Inc. All rights reserved. All trademarks
are the property of Thermo Fisher Scientific and its subsidiaries unless otherwise specified. PAXgene is a trademark of PreAnalytiX, GmbH.
TaqMan is a trademark of Roche Molecular Systems, Inc., used under permission and license. CO210394 0715
Measure gene expression with exceptional
ease, speed and accuracy
Real-Time RT-PCR without RNA purification
Cells-to-CT technology guide
Invitrogen Ambion Cells-to-CT kits allow you to skip
RNA purification by going straight from cultured cells,
96 TaqMan
Assaysto
: 16,500
Jurkat
Cells gene
blood
or single
cell samples
measuring
relative
45
expression by real-time RT-PCR (qRT-PCR). Cells40
to-C
T kits are available in both Applied Biosystems
35
TaqMan
and Applied Biosystems SYBR Green
formats
and offer:
30
™
™
™
Cells-to-CT Average CTs
™
™
™
™
25
•Extraordinary
ease and speed—96 samples for
qRT-PCR
in
typically
<10 min
20
TaqMan Gene
Expression
Cells-to-CT Kit
Cat. No. 4399002
•Superior performance—designed for consistent
accuracy, reproducibility and sensitivity with
10–100,000 cells
TaqMan MicroRNA
Cells-to-CT Kit
Cat. No. 4391848
•Validated kit workflows—complete sets of reagents
preoptimized to work efficiently right out of the box
Single Cell-to-CT
qRT-PCR Kit
Cat. No. 4458237
cDNA archiving
TaqMan detection
chemistry
miRNA &
IncRNA
expression
10
15
20
25
30
35
40
Start here
(choose analysis type)
45
Skip RNA purification
without
compromising
performance
Purified
RNA Average
CTs
Performance of the Invitrogen Ambion Cells-toSensitivity of the Cells-to-CT 1-Step TaqMan Kit is
CT 1-Step TaqMan Kit is tightly correlated with a
better than a traditional approach using purified RNA.
traditional approach
using
purified
RNA.
TaqMan Assay Correlation
™
™
Gene
expression
from cultured
cells
Limited sample
Single Cell-to-CT
qRT-PCR Kit
Cat. No. 4458237
™
Gene
expression
from stabilized
blood
™
SYBR detection
chemistry
cDNA archiving
35
40
33
31
24
20
27
15
y = -3.5328x + 37.427
R = 0.99864
y = -3.669x + 36.879
R = 0.99972
21
Choose a 1-step kit, if you:
•Do not store cDNA
19
10
15
20
25
30
35
17
40
0
1
Data from 72 assays compares performance of the Cells-to-CT 1-Step
TaqMan Kit with a traditional approach using purified RNA. Lysate from
16,500 Jurkat cells was prepared for qRT-PCR on the Applied Biosystems
7900HT Real-Time PCR System.
2
3
4
5
•Dispose of samples after one or a few uses
6
log(10) Number of HeLacells per lysis
Purified RNA average Cts
Power
SYBR Green
Cells-to-CT Kit
Cat. No. 4402953
25
23
y = 0.9465x + 1.5865
R = 0.97682
Blood-to-CT Kit
for qPCR
Cat. No. 4449082
XENO Cells-to-CT Kit
XENO puri ed RNA
ACTB Cells-to-CT kit
ACTB puri ed RNA
29
30
average Ct
Cells-to-CT average Ct s
35
10
Cells-to-CT
1-Step
TaqMan Kit
Cat. No. A25605
y = 0.9862x + 0.5252
R = 0.96308
15
10
•No tedious RNA purification—no columns, heating or
centrifugation
•Have many samples with one or a few targets
™
HeLa cells (10 to 100,000) were processed in triplicate using the Cells-toCT 1-Step TaqMan Kit or RNA purified with an RNA spin column method.
Each set of samples was analyzed by qRT-PCR for β-actin and XenoRNA
targets using the Invitrogen Ambion Cells-to-CT Control Kit on the
7900HT Real-Time PCR System.
Find out how your Cells-to-CT workflow can be automated at
thermofisher.com/autocellstoct
•Use liquid handling robotics
™
™
™
™
•Need to reduce the chance of cross contamination
•Need to reduce time-to-results
To find out more and to order, go to thermofisher.com/cellstoct
Cells-to-CT
1-Step Power
SYBR Green Kit
Cat. No. A25601

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