T.37 PALl.MAlI.I10HHAH EI10JIOfI1H. PALI..I103KOJIOfUR PAJ

T.37 PALl.MAlI.I10HHAH EI10JIOfI1H. PALI..I103KOJIOfUR PAJ

PALl.MAlI.I10HHAH
EI10JIOfI1H.
T.37
PALI..I103KOJIOfUR
1997
Bblfi.
PAJ],HALJ,HOHHA5I
I
fEHETHKA
Y,IJ,K 577.346:577.217+582:539.1.047
© 1997 r.
{as! pq,f
C.A. fepacbKHH,
E.R
3H6JHf~KaH,
A.A.
Y,!J,aJIOBa
3AKOHOMEPHOCTI1
BbIXOLtA CTPYKTYPHbIX
MYTAlI.l1M: B KOPHEBOli
MEPHCTEME 06JIYl:IEHHbIX
.I10HM3I1PYIOIU:I1M 113JIYlJEH.I1EM
CEMRH HIJMEHH
c-
YI~Y'ICHbI JaKOI!OMepHOC1'II
IJbIXO)lCl aocppaI,IIl1
XpOMOCOM, I!arpY)KCHHOCTH
KnCTOI-.: lIOBpC)KHCHHSIMI! I! I!~MCHCHHH cnCK1'pCl CTPYK1'YPHbLX MY1'Cll\lIil B KOpHCBOil
~1cpllCTeM(, CCMHI! H'IMCHH B 3aBHCII~fOC1'H 01' )10%1 06nY'ICHHSI. BblSlIWCH Y'laCTOK
KOToporo
'laC1'01'3 36cppaH1'Hbix
),O'30BOII 3aBHCHMOCTII (1-10 rp), B npc)lcnax
KnC1'OK C1'a1'HCTH'ICCKIf )lOC1'OBCpHOnpeBblwacT
cnoHTaHHblH yponeHh " HC 33BHClIT
01' IJCJ1If'IIIHbl AO~bJ.
]10H1I311PYI01I(ee 1I3J1Y'teHlIe, a6eppGl(lIll
XpO.MOCOA!, HozpYJ/CeHHOC/1lb
(}cHlIfWII, He.111He(lHocmb o030f3oil 3Uf3UCUAIOCnlll.
HcnOflb3YCMbiC
~
nOHxOAbl
B HaCTOHIllce
BpeMH AflSl OL\eHKu reHCTlf'leCKI1X
OCHOBaHbl Ha ITI1HeffHOH 3KcTpanOflSlL\HH
CpC)J,HHX A03 IJ 06flacTb
ManbIX.
(lOTC3C MO,L\CJIH He ItMCIOT
TlIBOpC'Iue
rIpO'IHOro
C HMelOIllHMHCSI
KOppCKTHOfl
6UOflOrl!'JeCKoro
3KcncpHMeHTaJIbHbIMli
OL\CHKH reHCTII'leCKItX
IlOC.'IC,L\CTBI1(106.'IY'ICHWI
6HOJIOnf'ICCKHX
O)J,HaKO 6a3IfpYKHl\HeCSI
hJ1emOK 1l08pCJ/('
3cpcpeKToB
Ha JlIHlefIHoii
060CHOBamUI
,L\aHHhiMli
1I0CJIe)J,CTBHH 06flY'ICHHH
CBH:3aHa C BOCCT3HOUJICHltCM I1CTlIHHOH cpOPMbl A030BOI!
:JHa'ICHHH 3KCII0311l\HH.
r]
J.
60.'IbUIIIX II
6ecnoporouofl
nt-
It BXOAHT B IJpO-
fT03TOMY
npo6.'ICMa
B 3Ha 'I!1Tefl bHOI"! CTencHII
3aBHCliMOCTI1
B 06fl3CTH
M3.'IblX
IlpH
HCCJICAOB3HI1H 33KOHOMCpHOCTCH I1HAYKl\HH CTPYKTypHblX
MYTal\Ul1 B KOpHCBOii
MepHCTCMC 06nY'leHHb1X nOHH311pYIOIllHM M3.'IY'leHHeM CeM5IH reKcanflOHAH0I1
mlICHHI~bl [2]
HaMH Obl.'IO YCTaHoBflcHO,
4TO 3aBHCHMOCTb Bblxoga
rCHCTH4CCKHX HapywcHHH
1I0CIIT HCflHHefIHhIl1
x3paKTep.
DhJJIa TaK)I(e
BhlCKa3aHa
rMllOTC3a
0 TOM,
oonY'ICHHH
ypOlmc~r
Ce~!HH Apyn!x
CIlOHT3HHoro
ccnbCKOX0351HCTBeHHbIX
MYTarCHC3a,
MO)l(CT
KYJIhTYP, xapaKTepH:JylOll\UXCH
6blTb
o6HapY)KeH
Y'laCTOK
01' )10%1
41'0 npll
MeHbWIlM
A030BOll
33811-
CII~IOCTI!, B IJpCjJ,Cflax KOToporo
'laCTOTa
reHCTlI'ICCKI1X
HapYUleHHI1
CTaTHCTI!4eCI<II
j\OCTOIJCPHO llpCBblUlaCT
cnOHTaHHblH
ypoaeHh
If He 3aBI1CIIT 0'1' neJJIi'IHHbl
.A03bl. g.~j!
npOBCpKI1
cnpaBCAJIHBOCTIi
3TOH rUllOTe3bl
HaMII 61,11111113Y'ICHhl 3aKoHoMepHocni
CTPYKTypHhlX
MYTaL\HH B KOpHCBOH MepHcTeMe
(0,1-300 rp) AHana30HC .A03.
ceMSlH jl'{MeH~,
o6flY'leHHb1X
IJblXO)\a
B WHpOKOM
MATEPHAJIbI If METO,!J,HKA
IICCJIC)J,OBaHHHCJIY)!(HJIli ceMeHa H'IMeHSI Hordeum
vulgare L. COpT 3a3epCKHI\
85. 06'bCKTOM
B03AYUlHo-cyxIfe
ceMeHa 06JJY'I3JIlf Y-KBaHTaMll
60Co (ycTaHoBKa
«faMMa-L\eflJJ-200»
0, I; 0,5; 1; 5; 10; 25; 50; 100; 200 II 300 fp. npn Bbl60pe
.Aliana30H3
)\03
B A03ax
,V)
0_
,~ ••
•••
----
..;;;;;;;;;;;;;=-==
'~_..••
""""ii·
","
••~-'~
"-ii"ii~IiiiI
__
••
"'·.wiiJ""-·IHliL_·-::
••;;"' ••"
rrpHHHMaJIOCb BO BHHMaHIle, '11'0 rrOKOSlll\HeCSI CeMeHa - HaH60JIee pa,[lHOpe3HCTeHTHaSl cpa3a
OHTOreHe3a
BblCWllX
paCTeHHH.
TaK, ,[IJISI KJIaCCa O,[lHO,[lOJIhHble,
TllmFlHbIM
rrpeACTaBIITeJIeM KOTOpOrO
SlBJISlIOTCSI3JIaKI1, pa,IJ,1l0pe3HCTeHTHOCTb
4,8-5.6
'leM
pa3a Bblwe,
24
CnycTSI
'l nOCJIe o6JIY'leHI1S1
20-40
KopewKoB
cnHpTe
(3 : I).
maBHoro
ceMeHa
npopall\HBaJIH
p,aBJIeHhle
npenapaTbl
KOpHSI 11OKpaWHBaJTH au,eToopceliHOM.
aHa-TeJIocpa3Hble
cneKTpa
KJIeTKI1 H Y'IIIThIBaJTI1
a6eppal\HH
(OAIIHO'lHbIX)
CeMSlH B
B '1aWKaX
TIeTpl1.
06pa3u,hl
no
,IJ,OCTllrWlIX )J;JTI1Hbl5-10 MM, cpl1KCl1pOBaJIH B au,eToYKcYCHOM
Ha Bapl1aHT,
BpeMeHHhIe
B cpa3e nOKOSlll\llXCSI
[3].
B cpa3e BerenlJ,lUI
npOBOp,HJIl1
11 XpOMOCOMHblX
no
rOTOBHJIl1
113 anl1KaJIbHOM.
B Ka)Kp,OM npenapaTe
,[IOJTIO KJJeTOK C a6eppau,llSlMH
[4]
KJIaCCllcpllKal\l111
(p,BOHHbIX)
MeplICTeMbl
aHaJI1l3HpOBaml
C BblJlCJIeHHeM
MOCTOB 11 cpparMeHToB,
Bce
XpOMOCOM; aHaJIH3
xpoMaTHAHhlX
a TaK)Ke
oTCTaBaHI1H
XpOMOCOM (reHoMHble
MYTal.l,lll1).
TIpl1 OlJ,eHKe oTC;TaBaHI1H Y'II1TbIBaJIH
XpOMOCOMhI,
JIe)Kall\l1e OT,IJ,eJIbHO 01' pa30Wep,WI1XCSI aHacpa3Hblx wanOK Ha paCCTOSlHI1H, He MeHee qeM B
ABa pa3a npeBbIwalOll\eM
TOJIll\I1HY XpOMOCOMbJ.
PE3YJIbTATbI
1I3MeHellue
'IaCTOTbI
a6eppaHTHblX
AaHHhle 0 BbIXO,IJ,e a6eppaHTHbIx
COOTBeTcTBYlOll\aSl
n03BomleT
npaKTll'leCKH
He 3aBllCliT
I
TIpe,IJ,Bapl1TeJIbHbIH
'11'0 BbIXO,IJ, a6eppaHTHblx
01' BeJIll'lHHbl
AHaJII13
TeJIbCTByeT
a TOM, '11'0 ,IJ,OCTOBepHble OTJIll'lHSI
npep,CTaBJTeHHbIX
TaHHoro
ypOBHSI penlcTpupYlOTCSI
Bblxop,a
a6eppaHTHbIx
Ha'lliHaSl
KneTOK,
[5].
3KcneplIMeHTaJIhHhlX
,IJ,aHHhIX
MeTop,OB
KonMoropoBa
""mO"Hn,
. !litI/-dti
I
/.
/
q
C?wrctlti
d.e.jJ.el2as
f)ose I
151
39
31
57
2\4
13
7
18
27
61
5
C ,[I03bI 1 fp.
-
60nee,
lIHTepBaJIOB
a6eppaHTllblx
OTCYTcTBl1e
A03e
5
fp,
KpHTepHSI llnH
H t-TeCTa
KJIeTOK
fp
CBlIAe01' cnOH-
OTJIU'IUH 01' KOHTpOJISI
nO-BuAuMOMY,
'11'0 npHMeHeHlle
CMl1pHOBa
Bcer~
npocMoTpeHo
KJIeTOK
r;-€
0,0
867
0,1
1179
0,5
828
1,0
2231
5,0
1051
10,0
1165
25,0
728
50,0
776
100,0
661
200,0
713
300,0
714
ee1I!g / /l
-(/Om
K/iemOK B KopNeBoii
:IIf!lt~zt
rp
TeM
1-10
CTaTHCTl1'1eCKHMH
He60nbillHM
P,Pyrllx
CBSl3aHO C
06"bCMOM
CTaTI1CTll'ICCKHX
CTblOp,eHTa)
n03BonSlCT
t3fr -;f?-RZC-S_Jt!-~.ft
>',TOCTaTHeT""ceKH )!oeTOBepHo" OTnH"He Bo<xo"a pa"HaAHoHHo-HHAYAHpoHa ••-
BblXOO a6eppawJlNbix
)],03a,
1 p,oBepHTeJIbHhIX
HCnOJIh3yeMoro
3TOH llHcpopMaLtl111
3TY rlinOTe3Y
'1aCTOTbl
cooTBeTcTBYIOll\erO
'1YBcTBHTeJIbHOCTblO
(KpHTepliSl
B Ta6JI.
aHaml3
:HaMH
a Ha pl1CYHKe -
KJTeTOK B p,llana30He
,IJ,03hJ. TIpoBepHM
MeTOp,aMI1.
HeAocTaTo'IHoH
1 npep,CTaBJTeHbl nOJIY'IeHllbIe
KJIeTOK. B n6JI.
KJIeTOK B 3aBHCI1MOCTI1 01' )J;03bl 06JIyqeHHSI,
p,030BaSl 3aBllCI1MOCTb,
npep,nOJTO)KllTb,
H OECY)KJ],EHHE
/1&t>f'lnt2
/'aaU2hDfz
MepllcmeMe
dL>SP",T
ceMflN Jl'lMellfl
om 003bl o6/1Y'leNllR
il /llC)UM
g-k7J1
"If
A6eppaHTHbie
l
"
KJleTKIi
aA/I?,r.agp / %
)],110.95
0,58
-/0
0,84
0,32
0,40
1,10
0,30
1,71
9,23±1,13"
1,75±±±±0,26
0,28"
01,45
,22
1,47
29,97
4,26
7,34
±
0,94"
0,75"
1,71"
21,18
\,53"
2,32
0,44"
0,07
0,51
++2,29
1.70
1,08
1.JHCJlOcpeAHee,
0,93
2,50
1,20
+++
33,33
26,61
7,02
11,44
5,51+9,18
2,79
18,l8
3,18
5,73
24,18
I
pa3J1H1.JHeC KOHTpOJ1eM 3Ha1.JHMO,p
a 6 n Ii l.\ a
B JaBllCllMocmll
/Jl1tfP/7A!/2L-
{~
< 0,05.
83
_
-"""
.. ".",
.
_
/he ~
mOl:..-
I
01.
r
~. C·
.~~
IUI· ~
...
elINtiW7(!g ;'oraIR/7VtteUR.t //2'?7ZEvlmm (i5af'~ ) ctejJ~/1cLt1i I/"on
radt L:'i-lioJl
a-Os-e .
o7,S11/
aJhrad
II
fNfMIl
r!.t
!JI
, __
%
e-uu
2,5
(;I
,
1-1/1-1_11
41
113MCHCHHC 'laCTOTbl a6cppaHTHblx
A03bl 06nY'lCHIISI
Do GCII a6cllllcc - A03a. fp;
H1.lX reHeTH'-leCKHX
CCMSlH BceMH
Ha'lHHaSi
rHnoTe3Y
a6cppal~Hi'I.
UH]lYllupoBaHH1.lx
YPOBCH1..
npaKTU'IeCKU
AOCTOBCpHblX
pa3JIU'Iltll
It cpaBHeHHeM
Ha6JI1o]laeTCSI pe3Koe
MC)I(]lY
B1.l6opKH
t
0,1
TO'lKaMIt,
fp
IwaTO
1)
Ha ]l030BO"
Pe3YJl1.TaT1.l
cOBoKynHOCTH.
cnoHTaHHoMY
B nOJI1.3Y
1-10
fp
cpopMa
3)
H
KOHCKHX 6060B,
xapaKTepa
KpOBH '1CnOBeKa,
3aBIICHMOCTH
[1])),
60JI1.llllfX
]l030BOH
nOJlY'IeHH1.IMlf
11031.1npC):1CTaBneHbl
OlleHKII
Ha APymx
1I0Bpe)l(AcHHH
CIIMOCTII
84
no pacTeHHSlM,
0 TOM, '1TO H3MeHeHHe
XOMHlJKa.
H T.iI·
B1.IBOA 0 TOM, 'ITO
~HToreHeTH4eCKHX
xapaKTepa
no-
06'beKTOB
3aBHCHMOCTH H
B03AeHCTBIDIM.
nOBpe)l(AeHHHMH
JlHlUb
KJIeTKH co
H3 aHaJIH3a
B Ta6J1. 2, a 3Ha4eHHSI Cpe):1Hero '1HClJa nOBpe)I(.QeHHH
Ha KneTKY
06'beKTaX
6b11IH
4HClJa nOBpe)l():1eHHH: Ha KJIeTKY B 3aBHCHMOCTll OT
KOTO?1.IX HCnOJIb30BaJlaC1. HHcpopMa~
cBII~eTeJI1.cTByeT
It cy-
):1a.1I1.HeHllleM aHa.1IH3e Y'IltTbIBa.1IHCb
pacrr03HaBaHHIO
HCKJIIO'IeH1.1. ,IJ;aHHble 0 pacnpe):1CJleHHH
fp.
OT cnOHTaHHoro
KJICTKH. B KOTOp1.lX YAaJIOCb HAeHTHcpm~HpOBaT1. KOJIH4eCTBO H THn a6eppallHlf.
CJIO>t<H1.IMH, He rrOAAalO~HMHCH
fp;
25
oAH03Ha'lHO
pa3JIH4aSlC1. A.1ISIpa3H1.lX
npOHCXOAHT H3MeHeHHe
B
;;.:
KHTaHcKoro
n03BOJIHeT CAeJIaTh Ba)l(HblH
H3 DeTl'Y.
~,5
CeJl1.CKOX03S1HCTBCHHbIX pacTeHHH
KOTop1.le OnpeACJISI/OTCSI HX '1YBCTBHTeJl1.HOCTblO K BHelllHHM
Myra~HH
-
]103 -
3aBlfCitMOCTit
OTJIHlJalOLllerOCSI
B1.lXOAa paAHallHOHHO-HHAYllHpOBaHH1.IX
tJHeJ)O crpYKTypHblX
AaHllblC.
TPH B1.160PKH,
CThlOAeHTa
cpH6p06JIaCT1.l
BPC)I(ACHHH OT A031.1 HMeeT YHHBepCaJI1.H1.lH xapaKTep,
A03, npH KOTOpblX
06'bCAHHHM
HCClJeAOBaHHH cpOpM1.1 3MnHpH'IeCKOH
aBTopOB,
JlHCTOBaSi MepHcTeMa
'1TO OHH SlBJISIIOTCS!
nOBpe)l(AeHHH
ypOBHlO
nJIaTO, AocToBepHo
no 3TOMY nOBoAY B pa60Te
JlHll1b 3Ha'IeHHSlMit
fp
B1.IXOA KOTOpblX
CcpOpMHpoBaH1.1
C nOMOll.{1.1O KpHTepHSI
B1.UIBJleHHOll B XOAe HaCToHll.{ero
(JlIIMCPOIlHT1.1 nepHcpepH'-IeCKOii
(CM. 0630p
nOA-
25
COOTBeTCTBYIOll\HC
nonaraSl,
reHCTltlJCCKHX
fp
HeJIItHellHOro
1-10
06pa30M,
ypOBHIO
-
IW30BOH 3aBHCltMOCTit C peYJl1.TaTaMH APyrHx
lIpOpOCTKIt
fp.
C A031.1
KJIeTOK B KOHTpOJIe. a TaK-
PaCCY)I(AaSi aHanOrwmo,
TaKHM
1-3
B1.l60pOK
lIiCCTBOBaHItSi B ]lltana30He
ypOBHSI.
ConOCTaBlJeHHC
1-10
A03aM
nOBpe)l(AeHlIll,
AaHH1.lX MO)l(HO 06'beAHHHT1.,
3aBHCltMOCTH
cpaBHeHItSi
cBIIAcTenbcTBYIOT
OTCYTCTBIIC
Ha1IHHaSi
yaCTOTa a6eppaHTH1.lX
1, 5 H 10 fp.
]l03aM
cooTBeTcTBYlOll.{lte:
cnoHTaHHblH
nnaTO.
B npeAenax
HHTCpBaJIOB.
Y1IaCTKa: BblXOA
npeBblllmeT
CTaTHCTlt1leCKIi AOCTOBepHO He pa3JIlf'meTCSI,
ltaCTSlMH OAHOll reHepaJI1.HOll
2)
]l030He3aBHCHMoro
]lOCTOBepHo
cooTBeTcTBY101l.lHMH
AOBCpHTCJI1.H1.lX
3KCnepltMeHTan1.HhiX
COOTBeTCTBYlOll.{lte
fp
fp,
06JIY'IeHlIII
aHaJ1J13a no A-
BeJIH'IHHe nOrJIOLlleHHOll ]1031.1.
0,5
H
10
MeCTO npH
CTaTHCTH'IeCKOrO
YBeJIH1leHHe '1aCTOTbl reHeTH'IeCKHX
nponopllHOHaJIeH
A03ax
1-10
H
nOCTOSlHH1.lM
nOCKOJI1.KY. COrJlaCHO BceM OlleHKaM.
)l(e npli
ypOBHH HMeeT
Pe3yn1.TaT1.l
1, 5
A03aMH
TIICp)l(AaeTCSI
]lanee
OT cnOHTaHHoro
C 1 fp.
0 HaJIHlJIUl B ]lHana30He
OCTaBaSlC1.
KneTOK. %
no OCIi 0PlIllHaT - 'IaCTOTa a6cppaHTHblx
TBep)l(AalOT
/1...<1-
KJICTOK B KopHeBoH MCpHCTCMC H'IMCHS! B 3aBHCliMOCTil OT
nOBpe)l(AeHHll
]l03aMH
atJs e)
11111)
-
06 3MnHpH4ecKoM
B Ta6JI.
cpeAHero
OT A03b1 HOCHT HeJlHHeHH1.lH xapaKTep.
3.
AHamn
npeACTaBJICHHblX
4HCJIa nOBpe)l():1eHlfH
CpaBHeHHe
Ha KJIeTKY,
pacnpe):1eJIeHHH
.AiJi!
'1HC,1a
.AaHHbL'(
Ha KJIeTKY B 3aB!!-
):10BepHTe.1I1.HbIX HHTepBa..'IOB.
f)iSlll/lill/c.'>/!
aa' ·mafU
~
ratilal/o/2
dLJS~ '
2
Ta611H1.J,a
PacnpeOeJleHlle
4?;-?7'"_
't/W
uI!! d¥-V2a!/
,
t:l
;~/
'1UCJla n08peJICOeHUiI Ha KJlemKY 6 3aOllCll.MOCmU om 003bt 06/lY'IeHllR
lIow maY'11
Bcero
/
f
al,t'~ertl/--rolZ.s
.,£
3
2
'p.e..r ~:
H3,HIIX C \.
\.
5
4
,
6
\.
7
8
cno)l(HbIe
a6eppa1.J,IIHMII
a
867
1179
828
2321
1051
1165
728
776
661
713
714
0,1
0,5
1,0
5,0
10
25
50
100
200
300
t'lha"1!Z-I!-,
c1-
5
13
7
39
18
27
3I
57
61
151
214
»,:1
2
I
10
7
10
19
10
36
48
-
-.
2
3
7
15
I
I
3
14
16
I
2
7
-
1
8a/'ttp
-112-
f-!d£d..:;,oI.t!.pe/ld/lVZ1J-
J13.MeHeHUecpeOHezo '1ltcJla n06peJ/CoeHuu It lIazPYJlCeHHOCmU a6eppaHmHbtX
.MepltCmeMbt CeMRllll'l.MeHlI
I
0,05
4
1
7
4
3
(0
6
17
35
68
T a 6
1I
.' ':i'ra
a6eppa-
TOK
1.J,HH
1.J,
a
;;-Om
3
/'C'tLi'tt:zI-/i'J/-:Jd4J;P
mea.!? tWl7Jt5er
t'Nnoitlt/Fr£
13cero Clm.~. .CpeAHee1/ 'IliCtO
'To-lui?
••
a6eppaH'
THbIX Kne-
H
KJlemOK KOpl/e80U
6 3a6ltCUMOCmu om 003bt 06JlY'Ielllt.1l,y>or
B'17
cero
?l1C¥:1f! {-
I
a
I
a1/~c:!J:t? t-t17i()lL/Z1 pI a;a7JJaflt?.S IU'ZLJi 6t-1-rtien ?W/l/'m:/U- cd!s
'l7lef1tSH
{ifOO"t
5
7
4
21
14
17
21
26
31
57
58
t!{)~&MLet:L
ttAer;--cd10llS
r
atfe,?mMi/1£1
ticr.mCalJJ.!
TIoBpe)l(l\eHHOCTb KlIeTKIl
' nOBpe)l(lIeHHH
~, (..
,
Id_
j)t"~
(1
Ha0-2)
KnCTKY
(,'-
jJ-fJ ;-{'~
{ /O-~
~,
- 0,66
44
II
1162
31
5±
644
63
45320
4124
2224
1047
770
8276
941
14
86
32
14
646
312
146
678
209
116
1175
9±
728
867'
20
1,00
1,81
0,43
0,87
0,47
1,25
0,43
1,08
2.52
19
±0,62
0.38
0,78
1,00
1,21
± 0,00
0,00
0,23
\,3
20
I20
0,37
1,42
1,40 ±±
0,52
0,49
2,04'
10,66
1.55
0,5!
1,22'
5,02'
1,49
0.72
7,0120±±2.13'
10,62
29,06
1,31
8,56'
48,83
1,89
1,92±±
0,54"
±0,40
0,69"
I
I
I
I
:.Pa31111'lHe 01' Bcex 1103 IIHana30Ha 0-10 rp 3Ha'iIlMO C J10BepHTellbHOli BepOHTHOCTblO 95%,~
_i'-Ia:/-/:>-Irh:ltlry
Pa3ml'IHe
nOBpe)!(AeHHOCTH 01' YPOBHH, HHJ1Y1.J,HpOBaHHoro npH 0, I fp,
3Ha'lHMO C
AOBepHTenbHoH BepoRTHoCTblO
95%.
npOBepKa
AaHHbIX
C nOMOll.\bIO
CHOHHoro
aHaJIH3a,
a TaK)!(C
CPHWieHTa KOppeJIHQHH
AaIOT
cpaKT
AHana30HC
paHTHbIX
TCCTa KOJIMoropoBa
He OTJIH'IaIOll.\eeCH
Me)!(AY A030H H 'IHCJIOM a6eppal.\HH
OTCYTCTBHR AOCToBcpHoro
0-10
KJIeTOK
[p.
-
Hco6xOAHMO
3HaQCHHH
oco60
nOKa3aTCJIH
YBCJIH'ICHHR
OTMeTHTb,
«cpcAHec
CMHpHoBa,
AOCTOBepHO
pe3ynbTaTbi
OT HYJIH 3Ha'leHlIe
perpecK03cp-
Ha KJIeTKY (r:::: 0, I 8) I10ATBep)!(-
'IHCJIa
nOBpc)!(AeHHH
'ITO B OTJIlflIHC
Ha KJICTKY
OT 'laCTOTbl
'IHCJIO nOBpC)!(AeHHH
B
a6cp-
Ha KnCTKY"
He
85
2 )
oopa'JYIUT
1-10
;(OCTOHepHO
rp,
OTJll1'lalOll\CrOCH
01' CIIOHTaHHOrO
3).
XOTH TCHJ(CHI~,lH K 3TOMY I1MCCTCSI(CM. Ta6J1.
'JaK.110'laeTCH
'1l:IIHbIX
B OC06CH HOCTSIX pacnpCACJlCHIIH
H'j aHamna
ypOBHH
f1pH'II1Ha
IIJ1aTO
cyncpIlOBpC>KACHHhIX
KJleTOK co CJlO>KHbIMH, HC nOAAalOll\I1MHCH
B AI1alla30He
3Toro,
1I0-BHAI1MO~IY.
KJlCTOK
H HCKJlIQ-
I1ACHTH<pHKa~lIIi
nOBpc)j(-
:~l:IIIIHMH,
Bl:pOWrHOCTHbJC
paCnpl:;ICJle
xapaKTcpHcTHKH
pacnpCACJlCHIUI
HUH. '3Ha 'JeHHSI MaTeManPICCKoro
a6cppa~HH
O>KIIAaHHH
110 KJlCTKaM
II .1I.HcnCpCHH)
(Bill(
CTaTIiCTli'leCKU
lIl:pa'3JIH'JIIMbl
KaK ):(!IH ypOBHH rCHCTli'lCCKOro
1I0BpC>KACHHSI, KOTOpb1l1: BblWC 61,1:1
TaK II .1I.JlSIypOBHH
ootnHa'lcH
«cnOHTaHHbIM»
(HHAY~HpoBaHHbIH
.1I.03aMIi AO 0,5 rp),
nonpl:JI\)lCH
HH, COOTBeTCTBYK)l1~CrO nJlaTO Ha .1I.030BOI1 3aBI1CliMOCTH
OG.1Y'ICHHC
IIcpBoii
>KC nocJle
nJlaTO
25
A030H
rp
] -] a rp),
(AHalla30H
Be.1l.eT K 113MeHCHHIQ
B xapaKTepe
pacnpl:,(l:!Il:III1H
II II JHa'lCHHSlX cpeAHcro
'II1CJla nOBpe>KAeHHH.
f1pli
3TOM H3Y'IacMbifi
!!OK,naTl:.lb
j(OCTOIICpHO npCBblwaCT
He TOJlbKO KOHTpOJlbHblH
ypOBeHb,
/10 11 3Ha'lC/lHH.
xapaKTCpIlJYKHI(HC
pl:'JY!lbTaTOII
BCC HCnblTa/l/lblC
pacnpc)(c.'lC/lHH
1I0BpC>KACHHH
;~OCTOIIl:pIlOC
B03paCTaHHC
I'l:KGII1.10HJ(HOii
CorJlaCIiO
n:oplIH
OCTaeTCSI OAHIiM
IIpOI(CCChl
lIapal(lIIl
[6J, npli
MIlUlCHIi
rlyaccOHa.
JJ:HK no
O.1l.HaKO pacnpeAeJlCHHC
MYTa~lIlI,
Ha KOTOpbIC
'ICM 60JlbIllCC
IIX B IICTIIHHhlC
I!hIXO)(
,
CTPYKTYPHbIX
IIhICl1KIIX
OTJlII'IHC
a6eppa~IIH,
paAllal(lIlI,
I1Il1CHIIl(bl
[2],
IIH)(Y~HPYIQIl\HX
a6eppaHTHbix
11\1CTlI K.'lCTOK a6cppal~IIHMH
JTIIX ilaHHhlX
3aKO/la
npO~CCCbI
f1yaccOHa.
HII
01' HOp110 KnCTKaM
.1I.CHCTBHH AOCTa,TOlJHO
I10BpC>KgCHIiH
CpeAHHe
B MOJlCKYJlaX
CHCTeMbI HMelOT
3Ha'IeHHH
)(.'1Hpa3HbIX )(03 06J1Y'ICHHH npcgCTaBJlcHbl
C 1I0MOll~blO KOMnJlCKCa CTaTIICTIIlJeCKIIX
'11'0 B
BbIBO.1l., cgcJlaHHblH
nOBpe>KACHHII
OTKJIIIKa 61i0nOrHlJCCKOH
KneTOK nOBpe~eHHJlMH.
4),
xapaKTepHCTHKI!
Aa>KC B CJlY'Iac
pe-
J1cCJleAOBaHlle
(Ta6J1.
He npOTHBOpC'IHT
nOATBep>K)(aCT
MHO>KCCTBCHHbIC
B.'1HSlHHCHa ¢OpMllpoBaHllc
npOI(CCChl BOCCTaHOBJICHIIH.
I1TOrOBblM
3Mnlipli'IeCKoe
no KJlCTKaM nOKa3aJIO
pacnpCJlCneHIiH
:tIlK. 3Ha'iIlTC.'1bHOC
Harpy*eHHoCTb
~BJIH~Cb
HTorOBOC
H3Y'IaeMOH
a HMeHHO:
AOJl>KHO COOT-
BJlIIHHHC OKa3bIBalOT
60JlbillC
(200 II 300 rp)
A03ax
aHa.'1113C 3aKOHOMCpHOCTCH
MCpHCTCMbl
n03
B npc.1I.eJlax
CYll.(eCTBCHHOC B11HH/lHC OKa3bIBaIQT
TCM
a6Cppal(HH
HH 3aKOHY raycca.
,\1<1.1hlh1ro JaKOHa npH 60JlhIllHX
IIpll
HccJlegoBaHI!}!
fl3J1Y'ICHIIH
KneTKaM
rp pacnpC.1I.CJleHI1C lJl1CJla 1I0Bpe>K)(eHHH no KnCTKaM
'J<lKOHY f1yaccOHa.
Kl1pIlCBOil
MYTa~HH,
3aKOHa pacnpCACJlCHIiH
0-100
lIa~1II paHcc
Ha
H ):IJlH
1I0Bpe>KACHHH, CKOJlbKO npo~eCCbl
A011>I<HOOTJlH'laTbCH 01' TCOpeTIIlJCCKOrO
craTIICTlI'lCCKoro
jtllalla30HC
[2]
.1I.CHCTBlIli pe)(KOIiOHH3IipYIOll.(erO
nOBpC>K.1I.CHI1H MOJleKYJI
I!OCCTaHOB,'1CHHH. f103TOMY
pacnpCnCJlCHHC
>KC, HeCMOTpH
BblBOA cnpaBcg11IiB
'11'0 cpCJlHee 'II1CJlO nOBpC>K.1I.CIIHHHC YBcmi'lHBaeTcH
ncpBIPIHbIX
'jaKOHY
Ha
Ii TCM
KJlCTOK. 31'01'
IIOK.naTC.'1CM. OTpa>KaCT HC CTonbKO KapTIiHY ncpBIPlHbIX
pCa.'1lnal~1I1I
1I0JlY'IeHHbIX
Y'IaCTKa.
pacnpCJ(c.'1CHI!\:
IIl:TCTBOBaTb
KJlCTKaM
vb
3).
(Ta6J1.
BnJlOTb.1l.0 KOH~a IIJlaTO xapaKTep
TaK KaK B XOAC npOBC.1I.eHHOrO HaMH paHcc
TaK;.Kl: (ibIJlO o(iHapY;.KeHo.
ill!"301ll:3aIlIlCHMoro
no
)(03 01' ¢OHOBblX
BblXOAa a6CppaHT/lblX
IIlIlCHHl(bl,
.11.025 rp
.11.031,1BnJlO~b
CJlcilYCT, '11'0 B AHana30llC
HarpY>I<eH-
B Ta6JI.
MeTO.1l.0B nOKa3aJI,
3.
AHaJII\3
lJTO B AHana30He
)(OCTOBcp/lorO
pOCTa
;IOJ 01' ¢OHOHbIX
no KOH~a n11aTO, T.C. BnJIOTb go ]0 rp,
tlarpY>KCtlHOCTII
a6cppaHTHbIx
KJleTOK HC Ha6J1IO.1I.aeTcH. Doncc
Toro,
CCTb TCHgeH~HSI K
OIIl>KCHIIK1 ypOBHSI nOBpC>K.IJ.cHHOCTHKJleTOK K KOH~Y I1J1aTO. COIIOCTaBJlCHl!e
IlalllCM
P~ICIIOpH>KCHIIII 3MnHpH'ICCKIIX
KOptll:HOII
MCpllCTCMC rCKcannOH)(HOH
CYlIlccTBoHaHlI1I
;(\13:
I)
Y'la~IKc
H o60llx
O(jIl\HX
tlcpT
IIMCIOIl\IIXCH B
AaHHblX 0 HarpY>I<eHHOCTI! KJlCTOK nOBpC>KACHHHMII B
nIlleHHl(bI
[2J
B 3aKOHOMep/lOCTHX
C·'1YtIaHX OTCy-rCTBYCT AocToBepHbIH
II SI'IMCHH (Ta6J1.
3)
cBHAeTCJlbCTByeT
0
HX H3MCHeHHSI B 113Y'-ICHHOM gHana30He
pOCT nOBpC>K.1I.CHHOCTH Ha Ha'la11bHOM
3aOHCIIMOCTH; 2) HMCCT MCCTO YMCHbIllCHHC 3/1alJCHHSI H3Y'-IaCMOH xapaKTepHCTIIKII
.10 ypOR101 KOHTpO.'1S1 npH A03ax,
6JIH3KHX K KOHI(Y A030He3aBHCHMoro
Y4aCTKa,
T.e. np"
~(i:l,Y'IC~III~
B g03C I rp
B CJIY'lac nWCHHl(hI
H npH .1I.03e 5 fp
B cJIYlJac
H4MCHJ<:
. ) j,\ npt:;(l:.1aMH J(030HC3aBHCHMoro
Y'IaCTKa HarpY>I<eHHOCTb B03paCTacT 3a C4CT nOHB.'letllIH
K,1CTOK C MHO>I<CCTBCHHbIMH a6cppa~mIMH,
npH
3TOM 3Ha4HTeJlhHO
'1I1C.10 K.ll:TOK co CJJO>I<HbIMH,HC nO.1l.JlalOUUfMHCSIpacno3HaBaHHIQ
B Ta6.'1,
Mb
5
conOCTaB.'1CHbI
6HOJIOrH'ICCKnC 3¢CPCKTbI,
YBcnH4HBacTcJ<
nOBpe>KJ(CHHHMH.
HHAY~HpoBaHHblc
06J1Y'ICHHCM cc-
u __
>~.::-_-~
-"'~'r'" ~ n_,_-_-~ __
';fYoCamure
dlnw/4/Y/S
v
tD/'-
a/sf/'{ltL.:b'O/l!>
~ pm/EriC
am.otVtls fJff cdZ
a (!..UJ rda/z £-e
.A'1-
-_::../'t.~·_-.·-.·',-,i
l1J
/-1-1
tll?cL
JJLlCtSSOJ/
[;cutss
tLJw S ..
T a () .1 II Il a
P/!JYJ1bmambi
IIpOBepKII
COlJlCICIIH
C
.).\HWfill'leCKIIX
pacllpe(k.Jt'lil/l1
pacllpedl'c7t'HIIH.lfI/
nyaccolIlI
II
'IlICcW
1I0lif!t'.H(()cI/l/lI/1tl
LJ,03a,
(j
fp
I
I
DrryaccoH
D- Pl!Ct~;;2i(/1
0
0,]
0.5
1,0
5,0
10,0
25,0
50,0
100,0
200.0
300,0
o.om:
0,004
0.005
0,000
0.005
0,0 I ]
0,038
0,025
0.094'
0,158'
().007
0.014
0,0] 3
"0.021
0.040
0,033
0.020
0.182'
O.29! •
P-£Ct
U.
OOIllWKOBOAIY
xapaKlIlepy
OJ
Kf)
O,O(,()
O.())l)
(),O(H
0.063
0.064
d
nOBpelKAcHHoCTb
<I
TIyaccoH.
PLjGY5011
!
G;,auss
KJICTOK (,IJ,J10•95)
II
Pi./ClS5cY1
.
'.
itutnl4-)!
I
'1I1CJlO
nonpclKAcHHH
KJICTKY
MaKCHMaJIbHOe
'IIICJIOHanOBpeIKAeHHi't
Tun
pacnpellCJICHHSI
noepelKAcHHoCTb
KJICTOK (LJ,JII0.95)
-
III
(j)
( ~}~,,'
'T
f .;
f.JUS /'Itft
200, 30{) J'r f?t1J
He TIVaCCOIL Ill' ravcc
o
-A-mcUi1tof
dcrmllD.1>jhnOBpelKAcHHoCTb
p.e-.t-- cUPI
.
r
Will
-----.-nWCIIHl.\bl
npe)J.eJICHH~
nOBpC)!(ACHHH.
AJIH
lIalOl1I.HXCH BHAOM CTaTHCTH'ICCKOro
OOJIY'ICHHH A03aMH
060HI.IH
BnJIOTb
HCCJICAOBaHHblMIi
.'1l{)l-laJIOCb OTKJIOHCHHC
1lI - npH J\03C 100 rp
JJ.aHHbIM,
AJI5I ~'1MCH~
aHa.'10rH'/HO:
200
H
300
Y'IaCTOK
pacnpc)J.CJICHHC
CTaTIiCTll'lCCKIiMIi
pacnpCACJICHHH
HOpMaJIbHOC
xapaKTcp
6bU1O
pacnpCACJICHHH
AO 10 rp
l.fr2.::'
.
COOTBeTCTRYlOlIUIX
nWCHHl.\bl
G-CUIS ~
1.27
1.5-1.7
Of (].d,tJ
B A03ax.
yio
-
UwS£
6
1
KJICTOK (,IJ,JII0.95)-~ Dc(7Y/Cl~
H ~'IMeH~
-
fp
faycc
;Di1
m--".>THIJ paCnpCACJICHHSI'77iJp.e
mQ{IitICt,e J~rnO(~,i1+
MaKCIIMaJIbHOe 'IIICJIO nOBpcIKACHIiH®
CM-t~CWat-J ! ~ll1cJlO
nOBpclKAcHHij Ha KJICTKY
DF~
~({Ssin/ 7.X
0.] I-O.2t!
i1D
2.0--2.4
100
s 0;
L.!o.C(U
I.(}-I.X
10,11 !!2f!-.ctIlS5
1-'
5
1.2-1.9
fp
1l'J-
ray~,'
~O, 11
0.12-0.88
"0
J
rp
<0, 12
10.50
O)J.HHaKOBOMY
Bbl)J.l:JICHO
12]
3aKOHaMII;
II -
Jlpll
KaK 0'1' JlyaccoHoBCKOfO,
pacnpc)J.CJICHIiC
pacnpc):\CJICHHH
TpU
1I0BpCIKI-lCHUii
nOBpC)!(I-lCHIlII
XapaKTl:py
Y'laCTKa,
pacpaJ.'IIi-
I --.IIpli
JlO KJll:TKa~l:
XOpOll1O OIIiIChllla:IOCI,
J{O'Jax
I()
II
50
I'p
Hao-
T3K II 0'1' HopMa.1I,HOro:
OhlJIO aJJ.CKBaTHO )KCnCpIiMCHTa,'lhHI,I~1
nOBpcIK,1CHHII
110 K!1l:TKaM
I COOTBCTCTBYCT ,[1030BOMY ,[1Hana:JOHY 0--100 fp,
Y'laCTOK
Ml:HHCTOI
II -
;.lma~1
fp.
DPOBC,[1CHHOC
cpaBHcHHc
n03BO.'HeT
.111'lHe B BCJIH'IHHC
A03bl,
HHAYl\HpOBaHHblc
C'IHTaTh,
'1'1'0, HCCMOTpH
HOHII1HPYK1UlIIM
..5?-I!ed.!1
;'11 f!.-ti!!J.
110 1-:.·JI'III1·:a.1f
<2(X)
nyaC~\{H.
L1!f--
raycc
6
I
PUQSSOil
\
6amf/
~-:I:~~~~~:·_~~!!.
__
HC TIyacCOH. He raycc
(
and
:I
fA.
1J()(jfJe.J/(I)t'll/l/i
TIweHlIlla
° rp
,
Tun pacnpe).lCJICHIUI
MaKCIIMaJIbHOC 'IIiCJIO nOBpclKAcHllij
'1I1CJIO nOBpclKAcHIiH Ha KJICTKY
i
I.' :2.
3
.I ~ ~
5'
009
0.0):
O.OJ)
O,O)()
OJWX
pacllpeUC,lClJllH
IlVeaJ
Y'laCTOK
I
Q
_
0.0))
o.o-tx
,II < (!;05.
-o/ttel-s
cuor/1/3emCIIl6YIOI/(IlX
,(()
._I~~.~
/flCttlCR;d hi- IlracUClIr,~)1Z ~t-. ~f2CL_
q
~1./IUe.f1
CO(Ll1eSP()~-r
-tquctf! COJRII
a;Sfr-lla-hoT?a
IJd]14
II UC~I1?1Clt}e.S;
bI/OJ/u211'ieCKllec(r-e..
.J¢¢eKmbl,
IIHOY/(IIPOIWlllible t--v 06J/Y·/I'lIlIe.l(
1I1/1ellll/(1)1 II }/'L\II'I/J/
Ij ,JII.IllX.
(j
I
,
L
6.al-0~6
0.00::'
• Pa3~I1'IHC C TCOpCTH'h~~K!iM pacnpCACJlCHHCM ?Ha'IHMo.
; [}Iofoo<c<:/-R
tfl('Los,e~
-.-----.--------------
Dr"y,"
/)-
1,:,1<'11110.".'"
Tal'cca
-------------,--------------,------.-----,
f)DS~) Ge.j
-t
Ha CYIl.\CCTBCHHOC
H].1ytICHIIC~1
pa'j-
B Cl:~1CHaX III1IC'
X7
.
·_-;'-'jlr'''''''''''''·~''",,",''''
7ht
-m_S\
1- $P~c)-r- bf
c-haf'ac)-~$-Hc!S
6affly
for
'7nm'~Hm
J;-[-NLt-Wra1
r;Lf!?gtuji/~
1-11./)'711
roob
jf"f'adlaJ1!}! clDs.e
I t1.
m~(kd-t'DJt<;
laB
XapaKmepucmllKli
cneKmpa
cmpYKmypliblx
II." ••vt1u-e.,
et-rvt-O
7D~ LUL.r:L (11/1
,66 j6UCUMOcmu
D oS
t/
f'
L~
Myma1<lIl'i 6 KOpf/e6011 MeplicmeMe
J
t t~·
JJ;o3a, fp
g
Il\HH
6
R'IMeHR
om 003bl 06/iY'lelHiR
06ll\ee i'o~\' Ltnl-i tV
'l vT! cd-EHCt. iiDI1S.
tmeJIO
KOJlHtlee,\,Bo a6eppal\HH
pa3Hblx
a6eppaTHI10B/oIAfHNJ1. ..
Q.6er-/L
r:
R..e..
Ta6J1Hl\a
I
!
N
ta+( VI!.. 1 4- f)I u...e./1 c.P1
of
<'7
OTHocHTenbHa51 tlaeTOTa a6eppa.
l\HH pa3HbIX THI10B
Gh~JtI:
/LfJt....o;g f\-IY))-1' !...
".>rr
-f!.
611..5
m"
Ln ....
709
a7060,04
211
31
26
26
511
131
20,28
174
28
351
103711724
19
764
14
0
5963
00,50
12
44
0.55
114
0,00
0,02:
41
13
0,07
0,50
29
88
312
209
86
01]06
,40
0,60
0,26
0,67
0,18
,29
0,06
0,63
0,82
0,10
0,55
0,00
0,03
,21
0,01
0,23
0,51
0,08
0,59
0,01
0,04
0,0 '00,28
,09
0,09
0,05
0,40
0,00
Hilltblil S!'IMeHS!Ha Y'-IaCTKaXIII II 6HOJIOrll'leCKlle 3cpcpCKTbi 3KBilBaJICHTHbi no KpIITepH5IM
«MaKCIIMaJIbHOe 'IIICJIO nOBpC)!(AeHIIH Ha KJIeTKY», «CpCAHCC 'IHCJIO nOBpC)!(AemIH: Ha KJleTKY» H «HarpY)!(CHHOCTb a6eppaTHbix
KJICTOK». MHTepecHo
OTMCTIITb, '1TO BMCCTe e H3MCHCHIICM xapaKTcpa
pacnpCACJICHIIS! nOBpC)!(ACHIIH, COOTBCTCTBYIOIl.\IIMBblACJICHHOMY
CpCAHCC 'mCJIO CTjJYKTYPHblX Myrai\HH Ha KJICTKYBncpBblc CTaHOBIlTeH
HaMil y'!acTKY
60JIbWe CAHHIIi\bl [2]. B 3KcncpHMeHTc C S!'1MeHeM MaKcHMaJIbHbill 61I0JIOrlI'IeCKHll3cpcpeKT
no npeACTaBJIeHHblM B Ta6JI. 5 KpiiTeplIS!M He BblXOAHT 3a npCACJIbl Y'laCTKa II, T.e.
B03AelICTBHe Ha ceMeHa S!'1MeHS!A03aMH BnJ10Tb AO 300 fp OKa3aJIOCb HCAOCTaTO'lHbIM AJJH
IIHAYKi\HIIB KJICTKCKOJIH'IeCTBa nOBpe)!(AcHlIH, COOTBCTCTBYlO~cro Y'lacTKY III.
TIpH aHamf3C Ta6JI. 5 3aKoHoMepHo B03HHKaCT Bonpoc, '1CM 06YCJIOBJ1CHOMHoroKpaTHoe ~
pa3J1H'!llc A03, IIH,D,Yi\HPYIOI.l\IIX
B KJICTKaXnWCHIIl.\bl H S!'1MeHS!paBHoe KOJIlf'ICCTBO nOBpe)j(ACHHH. I1C'ICpnblBalO~ce
06'bS!cHeHlIe 3TOMY AaTb CJIO)!(HO,OAHaKO HeCOMHeHHO, 'ITO rJlaBHOll npll'lHHOH S!BJHleTCS!pa3J1iI'mC 3KcncpHMCHTaJ1bHbIX 06'bCKTOB. fCHOM MSlrKOll nweHHl\bl S!BJl5ICTCS!
nOJllInJ10HAHbIM [7], 'ITO 06YCJIaBJIlmaeT 60J1ce CJIO)!(HYlOopraHlI3al.\1I1O reHeTll'IeCKOro MaTepHaJIa no cpaBHCHHIO C AllnJIOIIAHblM rCHOMOM S!'1MeHS!H He MO)!(eT He oTpa-
Ill,
3llTbCS! Ha xapaKTcpe
OTBCTHOHpCaKi\HII Ha 06J1Y'teHHC. KpoMe Toro, IICnOJlb30BaHHblH B
Ka'IeCTBe 06'bCKTa B pa60Tc [2] COpT 3HIITa S!BJI~CTC~rn6pHAHbIM, 'ITO TaK)!(e BJIH5IeTHa
ero YCTOH'IHBOCTbK BHelllHHM B03Aeli:cTBIIS!MII BeJIll'IHHY HHAYI.\HpOBaHHoro 06J1Y'IeHHeM
3cpcpCKTa.
CneKTp a6eppanHH.
AHanH3 cneKTpa a6eppai\HH
npOBOAHJIH no KJIaCcH<pIIKai\llll [4]
C Bbl,D,CJlCHHeMxpoMaTll,D,HblX (OAllHO'IHbIC cpparMeHTbl f) H MOCTbl (m') XpOMOCOMHblX
(ABoHHble cpparMeHTbl (f") H MOCTbl (m")) H reHOMHblX (oTCTaBaHHS! XpOMOCOM (g)) MyTa·
Ll,HH. B Ta6JI. 6 npCACTaBJIeHbl AaHHblC 0 'IHCnC II OTHOCHTCJlbHOM BKJIaAe HACHTHcpmJ,lI.pOBaHHblX B 3KcnepliMcHTe
KJ1eTOK C onpCACJ1eHHbIM TlinOM MYTai\HH B 3aBIICliMOCTH OT
A03bl. npc06naAalO~HM
THnOM CTpYKrypHblX MYTai\HH S!BJlS!JIHCbxpoMaTHAHblC - 46-82'JC,
MeHbwylO AOJIlO COCTaBJIS!JIIireHOMHbie Myral.\HII - 9-52% H, KaK H B cJIY'Iac nlllCHHlJ,bl [2J.
caMYlO ManO'IHCJ1CHHYlOrpynny - XpOMocoMHbie a6cppaI.\HII - 2-10%. CpeAH xpOMaT1U{Hb1X
a6CppaI.\HH npeo6J1aAaJ111 OAHHO'IHbIC cpparMeHTbl. BblXOA a6eppaI\HH
BCCX THnoB ;:Iocr('BepHo YBCJIH'IIiBaeTcS! C A030H B AHana30He 0-300 fp. OAHaKo Ha Y'IaCTKe 0-10 fp T,n::o.-.:'
pOCTa He 06HapY)!(CHO: CTaTllCTH'IeCKHH aHaJIH3 nOKa3aJ1, 'ITO BblXOA reHOMHblX H XPO>';THAHblX a6eppal.\HH B AHana30HC 0,1-10 fp He OTJIH'IaeTCS! AOCTOBepHO OT KOHTpO:ib?C';'"
88
9-
It
ciU.C<..y..e.d;
(},tWO'rnOS1)111f2
(ff- fW1~e 1/fw...fftft'Dn)
(}J~ro1na+hu 11u~-Icd-(~
J1 7 (-I') -- $1~
GkfL{)1ntw-0111Jl.
1ntJ)-a..{,Oil
-'> {
f
1t)-dtJuUe.
~Wj
fra61 tfrlfJlfC;;
'\
•
{1Y' , ~ ~~ ~~
(11'"
__-:.
~ ----~...- ...
~