Supporting Information Figs S1–S4 and Tables S1–S7 Fig. S3

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Supporting Information Figs S1–S4 and Tables S1–S7 Fig. S3
Supporting Information Figs S1–S4 and Tables S1–S7
Fig. S3 Mycorrhizal colonization with G. aggregatum
(a) or R. intraradices (b) of non-shaded (white bars) or
shaded (grey bars) plants that were grown in systems
with two non-shaded plants (NS/NS), two shaded plants
(S/S) or one non-shaded and one shaded plant (NS/S).
Shown is the average of n = 5–10 ± SE. Different letters
on the bars indicate statistically significant differences
according LSD test (P ≤ 0.05). The results of the twoway ANOVA are shown in Table S6.
Fig. S4 Percentage of long-chained poly-P of the total poly-P
in mycorrhizal roots colonized with G. aggregatum (a) or R.
intraradices (b) in non-shaded (white bars) or shaded (grey
bars) plants that were grown in systems with two non-shaded
plants (NS/NS), two shaded plants (S/S) or one non-shaded
and one shaded plant (NS/S). Shown is the average of n = 5–
10 ± SE. Different letters on the bars indicate statistically
significant differences according LSD test (P ≤ 0.05). The
results of the two-way ANOVA are shown in Table S7.
Table S1 Results of two-way ANOVA: effect of shade treatment or fungal species on ERM
development in the fungal compartment of the growth systems.
Fungal species
Shade treatment
Fungal species  Shade treatment
F
F1,11= 2.8578
F1,11= 0.0364
F1,11= 0.0001
P
0.119
0.8521
0.9921
Table S2 Results of two-way ANOVA: effect of shade treatment or fungal species on 33P
contents in dpm mg-1 d.wt. in roots and shoots of Medicago truncatula plants interconnected
by a CMN (see also Fig. 3).
Root:
Factor
Fungal species
Shade treatment
Fungal species  Shade treatment
F
F1,50= 10.3113
F3,50= 2.6421
F3,50= 0.6032
Shoot:
Factor
Fungal species
Shade treatment
Fungal species  Shade treatment
F
F1,50=0.0149
F3,50=5.8961
F3,50=1.8986
P
0.0023
0.0594
0.616
p
0.0.9033
0.0.0016
0.0.1418
Table S3 Results of two-way ANOVA: effect of shade treatment or fungal species on 15N
contents in % of total N in roots and shoots of Medicago truncatula plants interconnected by
a CMN (see also Fig. 4).
Root:
Factor
Fungal species
Shade treatment
Fungal species  Shade treatment
F
F1,32= 4.235
F3,32= 5.7783
F3,32= 3.8623
P
0.0478
0.0028
0.0183
Shoot:
Factor
Fungal species
Shade treatment
Fungal species  Shade treatment
F
F1,32= 2.4332
F3,32= 3.1936
F3,32= 1.8809
P
0.1286
0.0366
0.1527
Table S4 Results of three-way ANOVA: effect of shade treatment, fungal species, or nutrient
addition to the FC on the expression of MtPt4 or 1723.m00046 in the roots of Medicago
truncatula (see also Fig. 5).
MtPt4
Fungal species
Shade treatment
Fungal species  Shade treatment
Nutrient supply
Fungal species  Nutrient supply
Shade treatment  Nutrient supply
Fungal species  Shade treatment 
Nutrient supply
1723.m00046
Fungal species
Shade treatment
Fungal species  Shade treatment
Nutrient supply
Fungal species  Nutrient supply
Shade treatment  Nutrient supply
Fungal species  Shade treatment 
Nutrient supply
F1,76=
F3,76=
F3,76=
F1,76=
F1,76=
F3,76=
F
29.0298
10.7434
4.5835
15.6099
7.528
2.3015
F3,76= 2.1213
F1,77=
F3,77=
F3,77=
F1,77=
F1,77=
F3,77=
F
23.57
2.5175
5.5765
28.7949
18.2394
2.9223
F3,77= 3.9984
P
<0.0001
<0.0001
0.0053
0.0002
0.0076
0.0839
0.1045
P
<0.0001
0.0643
0.0016
<0.0001
<0.0001
0.0392
0.0106
Table S5 Results of one-way ANOVA: biomass of root and shoots of non-mycorrhizal and
mycorrhizal Medicago truncatula plants of the 15N or 33P labeling experiment (see also Fig.
S2).
F
P
15
Roots, N
Treatment
F8,55= 3.9099
0.001
Roots, 33P
Treatment
F8,50= 1.4427
0.2024
Shoot, 15N
Treatment
F8,59= 0.629
0.7501
Shoots, 33P
Treatment
F8,54= 2.9104
0.009
Table S6 Results of two-way ANOVA: effect of shade treatment and fungal species on
mycorrhizal root colonization of Medicago truncatula (see also Fig. S3).
Fungal species
Shade treatment
Fungal species  Shade
treatment
F
P
F1,36= 5.4253 0.0256
F3,36= 27.3573 <0.0001
F3,36= 1.4638
0.2407
Table S7. Results of two-way ANOVA: effect of shade treatment and fungal species on the
percentage of long-chained polyP of the total root polyp in the roots of Medicago truncatula
(see also Fig. S4).
Fungal species
Shade treatment
Fungal species  Shade
treatment
F
P
F1,45= 23.1404 <0.0001
F3,45= 6.2312 0.0012
F3,45= 2.424
0.0781

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