Effect of Crude Extract of Moringa Oleifera Lam on Skin and Muscle

Transcription

Effect of Crude Extract of Moringa Oleifera Lam on Skin and Muscle
DOI: 10.21276/aimdr.2016.2.6.AT4
ISSN (O):2395-2822; ISSN (P):2395-2814
Effect of Crude Extract of Moringa Oleifera Lam on Skin and
Muscle Tissues of Abino Rats and on Pseudofolliculitis Barbae
in Human.
Omorodion, Nosa Terry1, Achukwu, Peter Uwadiegwu2, Ibeh, Isaiah Nnanna3
1
Lecturer, Department of Medical Laboratory Science, School of Basic Medical Sciences, University of Benin, Benin City, Nigeria.
Associate Professor, Department of Medical Laboratory Science, Faculty of Health Sciences and Technology, University of Nigeria, Enugu
Campus, (UNEC) Nigeria.
3
Principal Medical Laboratory scientist, National Veterinary Research Institute, Diagnostic Laboratory, Ibadan, Nigeria.
2
Received: October 2016
Accepted: October 2016
Copyright: © the author(s), publisher. Annals of International Medical and Dental Research (AIMDR) is an
Official Publication of “Society for Health Care & Research Development”. It is an open-access article distributed
under the terms of the Creative Commons Attribution Non-Commercial License, which permits unrestricted noncommercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
ABSTRACT
Variations
in the arterial
pattern
of the
upper
limbtoare
very common
as observed
in many
cadaveric
andofangiographic
Background:
Moringa
oleifera
Lam
similar
Moringa
pterygosperma
Gaertner
belongs
to family
shrubs
studies.
of variations
origin and
the radial
artery
important
theyregion
are used
andKnowledge
tree, Moringaceae
and in
is the
considered
to course
have itsoforigin
in Agra
andisOudh,
in thebecause
northwest
of for
many India.
diagnostic
as that
well as
and reconstructive
surgeries
like coronary
percutaneous
The procedures
Indians knew
thevascular
seeds contain
edible oil and
they used
them forangiography,
medicinal purposes.
coronary
intervention
and coronary
arterycited
bypass
Duringliterature
routine dissection
our institute,
we observed a case
Moringa
preparations
have been
in surgery.
the scientific
as havinginantibiotic,
antitrypanosomal,
of highhypotensive,
origin of theantispasmodic,
radial artery inantiulcer,
a 33 yearanti-inflammatory,
old male cadaver.hypocholesterolemic,
It was found to be unilateral;
on left side,
radial artery
and hypoglycemic
activities,
was taking
from considerable
3rd part of the
axillary
arterypurification.
at the lower
of literature
pectoraliswith
minor
before
the origin of
as wellorigin
as having
efficacy
in water
Theborder
scientific
respect
to moringa
subscapular
artery
and anterior
artery.
It had a superficial
course
the arm but
crossing
the median
continues
to grow
rapidly, circumflex
particularlyhumeral
in the area
of medicine,
human and
animalin nutrition
no previous
nerve work
from medial
to lateral
Theuse
further
course of this superficial
the forearm
has been
done side.
on the
on Pseudofolliculitis
barbae radial
(after artery
shaveinrash).
Hence,was
thisnormal
herbaland it
terminated
by forming
a deep
Palmar value
arch intohand.
These community
variations may
beremoval
of great of
clinical
implications barbae
for vascular
preparation
will be
of economic
the Nigeria
in the
Pseudofolliculitis
and plastic
andMethods:
radiologists.
course of
artery makes
it vulnerable
to to
accidental
injuries
(after surgeons
shave rash).
TheSuperficial
general objective
of radial
this research
work was
carried out
investigate
the and
elevates
the of
risk
of bleeding.
safety
moringa
oleifera on the skin and muscle tissues and to ascertain the medicinal effect on after shave
rash. The specific objectives were to: (i) investigate the degree of safety of Moringa oleifera on skin and
muscle tissues and (ii) investigate the curative effect of crude extract of Moringa Oleifera on Pseudofolliculitis
barbae (after shave rash). The photomicrograph from the skin biopsies taken from the albino rats reveals
that there were no histological changes to the tissues when compared with the control slide. Results: The
slides reveals no histological changes to the epithelia lining, inflammation or any form of histological changes
or cellular infiltration to the skin of the rats. The photomicrograph of skeletal, smooth and cardiac muscle
tissues reveals that there was no cellular infiltration , toxic effect or histological changes to the various
muscular tissues when compare with the photomicrograph from the control slides. Microscopically, both the
aqueous extract and boiled decoction did not cause any histological changes to both skin and muscular
tissues as revealed by the photomicrograph. The formed paste was able to effect total removal of the rash
when used on the consented individual within day 2 and day 3 of usage. Conclusion: Moringa oleifera
extract has the ability of removing after shave rash (Pseudofolliulitis barbae).
Keywords: Moringa Oleifera Lam, Pseudofolliculitis Barbae.
INTRODUCTION
Moringa oleifera Lam similar to Moringa
pterygosperma Gaertner belongs to family of
shrubs and tree, Moringaceae and is considered to
have its origin in Agra and Oudh, in the northwest
region of India[2].
Name
&&
Address
ofof
Corresponding
Author
Name
Address
Corresponding
Author
PinkiRai
Omorodion, Nosa Terry
Demonstrator,
Lecturer,
Department
of of
Anatomy,
Department
Medical Laboratory Science,
SHKM
Govt.
Medical
College,
NalharUniversity
(Nuh), India.
School
of Basic
Medical
Sciences,
of Benin,
E Benin
mail:[email protected]
City, Nigeria.
The Indians knew that the seeds contain edible oil
and they used them for medicinal purposes[1].
Moringa preparations have been cited in the
scientific literature as
having antibiotic,
antitrypanosoma[1], hypotensive, antispasmodic,
antiulcer, anti-inflammatory, hypocholesterolemic,
and hypoglycemic activities, as well as having
considerable efficacy in water purification[1,2]. The
scientific literature with respect to moringa
continues to grow rapidly, particularly in the area
of medicine, human and animal nutrition but no
previous work has been done on the use on
Pseudofolliculitis barbae (after shave rash). Hence,
this herbal preparation will be of economic value to
the Nigeria community in the removal of
Annals of International Medical and Dental Research, Vol (2), Issue (6)
Page 15
Section: Anatomy
Original Article
Omorodion et al; Effect of Crude Extract of Moringa Oleifera Lam
MATERIALS AND METHODS
Standard method of extraction was used in the
course of this investigation. The leaves were
collected, washed and cleaned properly and place
in a flat bottom flask before application of heat. To
obtained the boiled decoction 1 litre of distilled
water was added to 500g of freshly collected leaves
of Moringa species and allow to heat for 20
minutes, using gas cooker. After the extraction is
done by heat, the decoction was left to cool and
filtered using Whatman No 1 filter paper and was
kept in a refrigerator at 4 degree centrigrade for the
period of the research. The leaves of moringa plant
was collected and dried under shade and grounded
into powder. The powder (200g) were collected and
macerated in 70% ethanol at room temperature in
24 hours. It was then filtered using Whatman No1
filter paper. The extract was kept in air tight bottle
and refrigerated at 4oc until used. 50g of moringa
powder was taken for phytochemical analysis.
Twenty albino rats were used for this investigation
and they were grouped into four with five rats per
cage. Moringa plant was applied to the skin of the
albino rats for 20 days and the skin and muscle
biopsies were taken and processed and stained
histologically. The tissues were stained with
Haematoxylin and Eosin and photomicrography of
the skin and different muscle tissue of the albino rat
were taken. 500g was macerated in 200ml of the
crude extract to constitute a paste. Hundred persons
with Pseudofolliculitis barbae (after shave rash)
who consented to participate were recruited for this
investigation after obtaining ethical clearance. The
Moringa paste was used on consented persons with
after shave rash leaving around Uselu metropolis in
Egor local government Area of Edo state. The
extract was used on after shave rash on their skin
for the period of 2-3 days and observations were
recorded. Photographs of the consented persons
with after shave rash before and after used of the
moringa paste were taken.
glucosinolate, vitamin A, βcarotene, sucrose, Dglucose, flavonoid pigments such as alkaloids,
kaempherol,
rhamnetin,
isoquercitrin,
kaempferitrin, wax; calcium and potassium both of
which are essential nutrient in the body. The
photomicrograph from the skin biopsies taken from
the albino rats reveals that there were no
histological changes to the tissues when compared
with the control slide. The slide reveals no
histological changes to the epithelia lining,
inflammation or any form of histological changes
or cellular infiltration to the skin of the rats. The
photomicrograph of skeletal, smooth and cardiac
muscle tissues reveals that there was no cellular
infiltration , toxic effect or histological changes to
the various muscular tissues when compare with
the photomicrograph from the control slides.
Microscopically, both the aqueous extract and
boiled decoction did not cause any histological
changes to both skin and muscular tissues as
revealed by the photomicrograph. The formed paste
was able to effect total removal of the rash when
used on the consented individual within day 2 and
day 3 of usage.
Figure 1: showing Hair follicle (A) of skin tissue
before use of crude extract of Moringa plant stain
with H&E X400
RESULTS
The phytochemical analysis of Moringa oleiferalam
reveals the following; Niazimicin, Benzyl,
isothiocyanate,
Pterygosperm,
Benzyl
Figure 2: showing Dermis and Epidermis (A and B)
respectively of skin tissue before use of crude extract
of moringa plant stain with H&E X400
Annals of International Medical and Dental Research, Vol (2), Issue (6)
Page 16
Section: Anatomy
Pseudofolliculitis barbae (after shave rash). The
general objective of this research work was carried
out to investigate the safety of moringa oleifera on
the skin and muscle tissues and to ascertain the
medicinal effect on after shave rash. The specific
objectives were to: (i) investigate the degree of
safety of Moringa oleifera on skin and muscle
tissues and (ii) investigate the curative effect of
crude extract of Moringa Oleifera on
Pseudofolliculitis barbae (after shave rash).
Omorodion et al; Effect of Crude Extract of Moringa Oleifera Lam
Figure 3: showing Hair follicle (A) of skin tissue after
use of crude extract of moringa Plant stain with H&E
X400
Figure 4: showing Dermis and Epidermis (A and B)
respectively of skin tissue. After use of crude extract
of moringa plant stain with H&E X400
Moringa preparations have been cited in the
scientific literature as
having antibiotic,
antitrypanosomal, hypotensive, antispasmodic,
antiulcer, anti-inflammatory, hypocholesterolemic,
and hypoglycemic activities, as well as having
considerable efficacy in water purification. The
scientific literature with respect to moringa
continues to grow rapidly, particularly in the area
of medicine, human and animal nutrition. Spraying
the leaves of plants with the Moringa extract
prepared in 80 % ethanol and then diluted with
water produced some notable effects such as a
longer, more vigorous life-span, heavier roots
stems and leaves, bigger fruits and higher sugar
levels etc. The extract produces an overall increase
in yield of between 20-35 % based on data such as
the stem diameter, number of nodules, number of
axels, number of flower buds, and number of fruits
per flower bud. Moringa plants extract dissolve
readily in the solvents used in this work. The
solubility in water was much higher than in
alcohol. The alcohol extract of moringa plant
extract worked faster than the boiled decoction.
The plant extract was also used on after shave rash
and it removed the rash within one to three days of
usage. The skin of an albino rats stained with
Haematoxylin and eosin before and after usage of
Moringa plant extract show no traces of
histological changes or infiltration or inflammation
of any sort. The moringa oleifera lam extract
worked well on pseudoffoliculitis barbae, changes
were noticed within the day two and three of usage.
The use of the extract was on the skin and muscle
tissues was actually carried out to ascertain its
safety on the skin and muscle tissues.
CONCLUSION
Figure 5: showing intercalated disc of normal muscle
tissue after the use of Moringa plant extract stain
with H&E X400
In conclusion, Moringa oleifera extract has the
ability
of
removing
after
shave
rash
(Pseudofolliulitis barbae). The photomicrograph
taken from various slides reveals that the tissue
appears normal.
REFERENCES
Figure 6: showing striation (A) of normal muscle
tissue after the use of Moringa plant extract stain
with H&E X400
1. Anwar F. Latif, Ashaf M,Gilani A. Moringa Oleifera: a food
plant with multiple medicinal uses. Phytother. Res. 2007;
21:17-25
2. Foidl N, Makkar HPS, Becker K. The potential of Moringa
Oleifera for agricultural and indusrial uses. What
development potential for Moringa products?Dar Es Salaam.
2001.
3. Odee. Forest biotechnology research in drylands of Kenya:
the development of moringa species. Dryland Biodiversity.
1998; 2:7-8
4. Palada, M.C., Chang, L.C.,Yang, R. Y, Engle L.M.
Introduction and Varietal screening of drumstick tree
Annals of International Medical and Dental Research, Vol (2), Issue (6)
Page 17
Section: Anatomy
RECOMMENDATION
Omorodion et al; Effect of Crude Extract of Moringa Oleifera Lam
Section: Anatomy
(Moringa spp.) for horticultural traits and adaptation in
Taiwan. Acta Hort. 2007; 752:249-253
5. Proyecto Biomasa. Internal Report , UNI Managa. 1996.
How
this article:Das
article: Omorodion
NT, S,
Achukwu
How to
to cite this
A, Rai P, Das
MehrotraPU,
N.
Ibeh
Effect
CrudeArteryExtract
Moringa Case
Oleifera
Lam
High IN.
Origin
of of
Radial
A of
Cadaveric
Report.
on
and Den.
Muscle
of Abino Rats and on
Ann.Skin
Int. Med.
Res.Tissues
2016; 2(5):??:??.
Pseudofolliculitis Barbae in Human. Ann. Int. Med. Den.
Res.
2016;
2(6):AT15-AT18.
Source
of Support:
Nil, Conflict of Interest: None declared
Source of Support: Nil, Conflict of Interest: None declared
Annals of International Medical and Dental Research, Vol (2), Issue (6)
Page 18

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