Understanding, diagnosis, and treating human disease with light

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Understanding, diagnosis, and treating human disease with light
1
“Understanding, diagnosis, and treating human disease with light”
PI: YongKeun Park
Department of Physics, KAIST
Understanding disease
Cellular imaging
•
•
Diagnosis disease
Optical
Field
Measurement
Optical
Field
Control
Tissue tomography
•
Biomaterial-Photon
Interaction
Treating disease
Super resolution lens
Photobleaching-free imaging
Control multiple scattering
Active holographic
optical tweezers
•
Optical control of complex objects
2
Super-resolution live-cell imaging
based on diffusion-assisted FRET
(Forster resonance energy transfer)
In collaboration with MC Choi, TY Yoon, MW Kim, Wondo Heo, Hyochirl Rhee (KAIST)
S-Y. Cho et al, Scientific Reports, 2013
3
a
Target structure
labeled with donor
Conventional
imaging
sample
excitation
lens
filter
obj. lens
donor
b
dichromic
mirror
Localizing blinked subset of donor
via diffusion-driven FRET
Super-resolution
imaging
LED
illumination
lens
emission
filter
acceptors
mirror
CCD
camera
…
t1
t2
t3
S-Y. Cho et al, Scientific Reports, accepted
4
Widefield
a
dSOFI
+deconvolution
dSOFI
1 µm
1
widefield:
dSOFI:
dSOFI(decon):
Intensity (a.u.)
b
0
-400
-200
0
Position (nm)
200
257 nm
114 nm
33 nm
400
S-Y. Cho et al, Scientific Reports, accepted
a
Widfield
i
dSOFI
5
1 μm
i
ii
ii
5 μm
b
Widfield
dSOFI
iii
iii
iv
iv
1 μm
5 μm
Intensity (a.u.)
c
1
Wide field
0.8
dSOFI
0.6
0.4
0.2
0
0
1
2
3
4
Position (nm)
5
6
7
S-Y. Cho et al, Scientific Reports, accepted
6
a
5 μm
0 min
5 min
10 min
15 min
b
S-Y. Cho et al, Scientific Reports, accepted
7
Acknowledgement
BMOL @ KAIST
Dr. Youngchan Kim
Dr. Jaeduck Jang
Junghoon Park
Kyoohyun Kim
Hyeonseung Yu
Jaehwang Jung
Kyorae Lee
KAIST
Prof. Yonghoon Cho
Dr. Choonghyun Park
Prof. Mahn Won Kim
Dr. Hyunjoo Park
Dr. Junwoo Jung
Prof. Yonghee Lee
Suresh group, MIT
• Monica Diez
• Ming Dao
• Prof. Subra Suresh
Levine Group, UCLA
• Mark L. Henle
• Tatiana Kurunikova
• Alex J. Levine
Weizmann Institute, Israel
• Thorsten Auth
• Samuel Safran
• Nir Gov
Univ. Twente, Netherland
• E. van Putten
• Allard P. Mosk
Spectroscopy Lab., MIT
• Timothy R. Hilman
• Zahid Yaqoob
• Kamran Badizadegan (Harvard)
• Ramachandra R. Dasari
• Prof. Michael Feld
National University of
Singapore
Rajesh Chandramohanadas
Peter Rainer Preiser
Asan Hospital, Korea
Hyoeun Shim
Sungsoo Jang
JaeHyun Lim
Funding
KAIST, Samsung Electronics, Korea government NRF (Brain Korea 21 project,
Basic Research Lab project, NRF-2012R1A1A1009082), Chungam Foundation

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