GARLIC AND HEALTH PROJECT

Transcription

1
Leafy Vegetables germplasm: stimulating
use
Contract number: AGRI-2006-0262
Agreement number: 001 AGRI GENRES 870/2004
E-mail report December 2009 – May 2010
(E-report 09)
Cesena 2009
2
General information
1.
2.
3.
4.
The technical and financial reports of the third year have been submitted to the Commission by
March. We received comments on the technical report in April and have submitted our reply to the
Commission by early May. Hopefully the Commission will accept the technical report soon, as after
the acceptance of this report the evaluation of the financial report will start.
The annual project meeting in November 2009 was hosted by UNIBO (Cesena, Italy). We would like
to thank Filippo D’Antuono and his students very much for their kind hospitality in making this
meeting to a success.
In June the annual WP coordinator meeting is planned at ProspecieRara (Aarau, Switzerland).
As a coordinator I would like to stress again that when you are disseminate material (scientific,
professional, web publications, articles in newpapers etc) you need to acknowledge the EU-GENRES
programme in this article via the following wording (see also FAQ 11); "Action AGRI-2006-0262,
Leafy Vegetables – stimulating use, receives financial support from the European Commission,
Directorate-General for Agriculture and Rural Development, under Council Regulation (EC) No
870/2004".
3
List of milestones & deliverables (only to be updated by the WP leaders)
Overview of deliverables
Deliverable
number
Planned
completion
Status
Description
Achieved
On going*
D01
30
Passport data of the four leafy vegetable (LV) collections donated
x
D02
48
Databases of the four LV crop groups developed and on the Internet
x
D03
48
Evaluation and characterization results in fixed formats linked to the
databases
x
D04
48
Safety duplication system of the LV collections established
x
D05
48
Gaps in the four LV crop collection groups identified
x
D06
06
Minimum descriptors for the minor leafy vegetables concluded
D07
45
800 accessions of lettuce characterized
x
D08
45
110 accessions of spinach characterized
x
D09
45
180 accessions of chicory characterized
x
D10
45
D11
45
Characterization data entered in a fixed format to be sent to the database
managers
x
D12
48
930 accessions of lettuce regenerated
x
D13
48
50 accessions of spinach regenerated
x
190 accessions of minor crops characterized
x
x
Not started yet*
4
D14
48
200 accessions of chicory regenerated
x
D15
48
70 accessions of minor crops regenerated
x
D16
12
Seeds of the selected material of the four crop groups sent to the partners
involved
D17
45
115 accessions of lettuce evaluated
x
D18
45
320 accessions of spinach evaluated
x
D19
45
265 accessions of chicory evaluated
x
D20
45
49 accessions of minor crops evaluated
x
D21
45
Evaluation data entered in a fixed format to be sent to the database
managers
x
D22
12
Seeds of the selected material of the three crop groups sent to the partners
involved
D23
45
40 accessions of lettuce evaluated for utilisation and marketing
D24
45
125 accessions of chicory evaluated for utilisation and marketing
x
D25
45
15 accessions of minor crops evaluated for utilisation and marketing
x
D26
45
Evaluation data entered in a fixed format to be sent to the database
managers.
01
Start-up project meeting
x
D28
03
Leafy Veg website is launched on internet and active.
x
D29
06
First WP coordinators meeting
x
D30
11
First annual project meeting
x
D31
14
First annual progress report
D27
x
x
x
x
x
5
D32
18
Second WP coordinators meeting
x
D33
23
Second annual project meeting
x
D34
26
Second annual progress report
x
D35
30
Third WP coordinators meeting
x
D36
35
Third annual project meeting
x
D37
38
Third annual progress report
x
D38
42
Fourth WP coordinators meeting
D39
46
Final annual project meeting
x
D40
48
Final annual progress report
x
x
6
Overview of milestones
Milestone
number
Planned
completion
Status
Description
Achieved
(month)
Start made selecting material used for the evaluation activities, developing
core collections if needed for lettuce and spinach
Start made requesting passport data for the spinach, chicory and minor
vegetable databases and lacking data for the Lactuca database (ILDB)
Material selected for regeneration and characterization of the four crop
groups
On going*
M01
03
x
M02
03
M03
06
M04
12
Material selected for the evaluation activities of the four crop groups
x
M05
12
Inventory of system of safety duplication of the collections made and
discussed during the second project meeting
x
M06
24
Passport data of most collections of the four databases delivered
x
M07
36
Databases with passport data for the four crop groups nearly finalized
x
M08
36
Part characterisation and evaluation data donated and start made including
these in the databases
x
M09
36
Safety duplication of part of the collections realized
x
M10
36
Start made identifying gaps in the collections
x
M11
42
Start made with making the databases available via the Internet
x
M12
42
Safety duplication of collections inside the project realized
x
M13
45
Most characterisation and evaluation data donated for inclusion in the
databases
x
M14
02
Start made developing minimum descriptors for the minor leafy vegetables
x
x
x
Not started yet*
7
M15
06
Material for regeneration and characterization of the four crops selected
x
M16
06
Regeneration of material of some crops started pending the growing
season
x
M17
12
Regeneration and characterisation for all crops started
x
M18
24
Regeneration, characterisation of germplasm of the four collections will be
continued
x
M19
36
x
M20
36
x
M21
36
x
M22
36
110 accessions of Minor crops characterized
x
M23
36
x
M24
36
x
M25
36
x
M26
36
x
M27
12
Material selected for the evaluation activities of the four crop groups (see
WP1)
x
M28
18
M29
36
70 accessions of lettuce evaluated
x
M30
36
x
M31
36
x
M32
36
Evaluations started
x
x
8
M33
12
Material selected for utilization and marketing
x
M34
18
Activities on utilisation and marketing started
x
M35
36
x
M36
36
x
M37
36
x
M38
45
x
M39
45
x
M40
45
x
M41
01
M42
01
M43
05
M44
10
M45
13
M46
17
M47
22
Detailed workplan of the project defined at the start -up meeting of the
project (time schedules for regeneration, characterisation and evaluation
will be established)
The project website will be developed
First WP coordinators meeting organised
x
x
x
The second project meeting (first annual project meeting) will be organised
to discuss the progress and discuss the first annual progress report
x
First annual progress report will be finalised and submitted to the European
Commission
x
Second WP coordinators meeting organised
Third project meeting (second annual project meeting) will be organised to
discuss the progress and discuss the second annual progress report
x
x
9
M48
25
M49
29
M50
34
M51
37
M52
41
M53
45
M54
48
Second annual progress report will be submitted to the European
Commission
Third WP coordinators meeting organised
Fourth project meeting (third annual project meeting) will be organised to
discuss the progress; this meeting will also review the overall progress of
the project (mid-term review) in order to assess whether the following set
of milestones have been fulfilled by the project
Third annual progress report including a mid-term review of the coordinator
will be submitted to the European Commission
Fourth WP coordinators meeting organised
x
x
x
x
x
Final project meeting to discuss the progress report of the fourth year and
the final overall project report of the project organised.
x
Fourth annual progress report and the final overall report will be submitted
to the European Commission
x
10
P0: Centre
Netherlands
for
Genetic
Resources
(CGN),
Wageningen,
the
The group at CGN is engaged in four workpackages (WPs), WP1: Documentation, WP2: Regeneration
and Characterization, WP3: Evaluation and WP5: Co-ordination. For WP1 and WP5 CGN is also WP
leader.
Workpackage 1: Documentation
General objectives:
 Development of crop databases for spinach, Cichorium and minor leafy vegetables and updating the
existing International Lactuca database.
 Prioritize accessions for regeneration, characterization and evaluation. If necessary, special selections
or core collections will be made for the evaluations of the different crops with the help of these
databases.
 Link results of the characterization and evaluation activities of the project to the respective databases.
 Establish a functional system of safety duplication of the leafy vegetable crops in cooperation with the
ECP/GR Leafy Vegetables Working Group.
 Identify gaps in the collections also in cooperation with the ECP/GR Leafy Vegetables Working Group,
to broaden the collections.
Persons involved at CGN: Rob van Treuren, Frank Menting
Milestones & Deliverables:
Deliverable date
Deliverable description
Status
CGN
Partner
responsible
Passport data of the four leafy vegetable (LV)
collections donated
Done
P0,P2,P3
Databases of the four crop groups developed and on
the Internet
Evaluation and characterization results in fixed formats
linked to the databases
Safety duplication system of the LV collections
established
Milestone description
Done
P0,P2,P3
Ongoing
Pall
Ongoing
Pall
Ongoing
Status
CGN
P0,P2,P3
Partner
responsible
WP1
Year 1
Year 2
Year 3
D01; month 30
Year 4
D02; month 48
D03; month 48
D04; month 48
D05; month 48
Milestone date
Year 1
none
none
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Start made selecting material used for the evaluation
activities, developing core collections if needed for
lettuce and spinach
Done
Pall
Done
P0,P2,P3
month 03
Start made requesting passport data for the spinach,
chicory and minor vegetable databases and lacking
data for the Lactuca database (ILDB)
Material selected for regeneration and characterization
of the four crop groups
Done
Pall
month 06
Done
Pall
month 12
Material selected for the evaluation activities of the four
crop groups
Inventory of system of safety duplication of the
collections made and discussed during the second
project meeting
Done
P0,P2,P3
month 12
Done
P0,P2,P3
Done
P0,P2,P3
Done
P0,P2,P3
Done
P0,P2,P3
Done
P0,P2,P3
Start made with making the databases available via the
Internet
Done
P0,P2,P3
month 42
Safety duplication of collections inside the project
realized
Ongoing
Pall
month 42
Most characterisation and evaluation data donated for
inclusion in the databases
Ongoing
Pall
month 45
month 03
Year 2
month 24
Passport data of most collections of the four databases
delivered
Year 3
month 36
Databases with passport data for the four crop groups
nearly finalized
month 36
Part characterisation and evaluation data donated and
start made including these in the databases
month 36
month 36
Year 4
12
Activities
1. What has been done: Extension and updating of the databases. Including new project data in the
databases. New functionalities for easier access to project data have been included in the databases.
Gap analysis for lettuce and spinach was carried out.
2. What will be done: Continuation of including characterization and evaluation data in the databases.
Preparation of a draft paper about the new databases and gap analysis in cooperation with GEVES
and IPK.
3. Bottlenecks: None.
Workpackage 2: Regeneration and characterization
General objectives:
To regenerate and characterize parts of partner’s genebank collections, for improving conservation and
utilization. High priority will be given to material to be used in the evaluations, because the total numbers
of accessions included in all collections needing regeneration and characterization are far too high to be
handled in the framework of this project.
Persons involved at CGN: Liesbeth de Groot, Rob van Treuren
Milestones & Deliverables
Deliverable date
WP2
Status
CGN
Partner
responsible
done
P3
D06; month 06
Minimum descriptors for the minor leafy vegetables
concluded
Year 2
None
Year 3
None
Milestone date
Status
CGN
Partner
responsible
Start made developing minimum descriptors for the
minor leafy vegetables
done
P3
month 02
month 06
Material for regeneration and characterization of the
four crops selected (see also WP1)
done
P3
Regeneration of material of some crops started
pending the growing season
done
P0, P1, P2,
P3, P5, P6,
P7, P8, P9,
P10, P13
done
P0, P1, P2,
P3, P5, P6,
P7, P8, P9,
P10, P13
Year 1
Year 1
month 06
month 12
13
Year 2
month 24
Regeneration, characterisation of germplasm of the
four collections will be continued
Done
P0, P1, P2,
P3, P4, P5,
P6, P7, P8,
P9, P10, P13
P0, P1, P3,
P4, P7, P10,
P13
Year 3
month 36
month 36
5 accessions of rucola characterized
Done
(161 + 284
acc.).
320
planned for
2010
Done
(17 acc.)
Done
(70 acc.)
P0, P1, P2,
P3, P5, P6,
P7, P10, P13
month 36
Done
(16 acc.)
P0, P1, P5,
P7
month 36
5 accessions of rucola regenerated
Done
(11 acc.)
P0, P1, P2,
P3, P5, P7,
P9
month 36
P0, P3, P5,
P9
Activties
1. What has been done: 17 rucola accessions were characterized in an experimental field trial, and the
data were included in the database. 16 accessions of spinach were regenerated.
2. What will be done: characterization of 320 lettuce accessions.
Workpackage 3: Evaluation
General objectives:
To evaluate germplasm of the leafy vegetable crops for pest and disease resistance and abiotic
characters for a much more effective utilization of the genepools. With the outcome of this activity
subsequent breeding will result in more sustainable production methods and a better quality product.
Persons involved from CGN: Liesbeth de Groot and Rob van Treuren
14
Deliverable date
Status
CGN
Seeds of the selected material of the four crop groups
sent to the partners involved
Done
D16; month 12
Year 2
None
Milestone date
Status
CGN
crop groups (see WP1)
Done
Partner
responsible
WP3
Year 1
Partner
responsible
Year 1
month 12
Year 2
month 18
Evaluations started
Done
P0, P1, P2,
P4, P5, P7,
P8, P9, P10,
P12, P13
Ongoing
P0, P5
Year 3
month 36
Activities
1. What has been done: Evaluation of 263 spinach accessions for 2 Peronospora pathotypes (Pfs1 &
Pfs4). Data were included in the database.
2. What will be done: Evaluation of 263 spinach accessions for 3 additional Peronospora pathotypes
(Pfs3, Pfs6, Pfs10).
Workpackage 5: Co-ordination
General objectives: The general objective of this workpackage is the effective management of the
project. Management of the project will take place on three levels: on the integral project level, on the WP
level and on the crop task level.
Persons involved at CGN: Chris Kik
15
Deliverable date
WP5
Status
CGN
Year 2
done
done
Year 3
done
Year 1
Partner
responsible
Year 4
done
P0/Pall
D37, month 38
D38, month 42
P8/P0
D 39, month 46
P4/P0
D40, month 48
Final annual progress report
P0/Pall
Milestone date
Status
CGN
Year 1
done
Year 2
done
Year 3
done
Partner
responsible
Year 4
done
Pall
month 37
Third annual progress report including a mid-term
review of the coordinator will be submitted to the
European Commission
month 41
ongoing
P8/P0
P4/P0
month 45
Final project meeting to discuss the progress report of
the fourth year and the final overall project report of the
project organised.
month 48
Fourth annual progress report and the final overall
report will be submitted to the European Commission
P0/ Pall
Activities
1. What has been done:
a. Annual meeting was perfectly organized by Filippo D‘Antuono and his students in Cesena in
November 2009. All partners presented the work carried out in the reporting period and an
overview and a discussion per WP was given by the various WP leaders. The project proved to be
well on track. Third annual technical and financial progress report was written in January-March
2010 and was submitted to the EU by March. The reaction of the EU was received in April and a
reply was written by medio May.
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2. What will be done:
a. Organizing the fourth annual WP meeting together with Prospecie Rara on June 23 in Aarau,
Switzerland
b. Revision/updating of the third annual technical and financial reports.
3. Bottlenecks: None
17
P1:
Warwick HRI, University of Warwick, UK (WHRI)
No information yet received
18
P2: Groupe d’Etudes et de contrôle des Variétés et des Semences
(GEVES), Brion, France
The group at GEVES is engaged in four workpackages (WPs), WP1: Documentation, WP2: Regeneration
and Characterization, WP3: Evaluation and WP5: Co-ordination. For WP3, GEVES is also WP leader.
General objectives :
 Development of crop databases for Cichorium
databases.
Persons involved at GEVES : Pascal Coquin (Valérie Cadot until November 2008)
Milestones & Deliverables :
Deliverable date
WP1
Year 1
Year 2
Year 3
None
None
collections donated
Status
GEVES
Partner
responsible
Done
P0,P2,P3
Done
P0,P2,P3
On going
P0,P2,P3
On going
P0,P2,P3
Year 4
month 48
month 48
month 48
Databases of the four LV crop groups developed and
on the Internet
established
month 48
On going
P0,P2,P3
Milestone date
Status
GEVES
Partner
responsible
month 03
lettuce and spinach
Done
Pall
month 03
Done
P0,P2,P3
Year 1
19
month 06
Done
Pall
month 12
crop groups
Done
Pall
month 12
project meeting
Done
P0,P2,P3
delivered
Done
P0,P2,P3
month 36
nearly finalized
Done
P0,P2,P3
month 36
On going
P0,P2,P3
month 36
On going
P0,P2,P3
month 36
On going
P0,P2,P3
month 42
Internet
On going
P0,P2,P3
month 42
realized
On going
P0,P2,P3
month 45
On going
Pall
Year 2
month 24
Year 3
Year 4
Activities
1. What has been done: validation and sending of evaluation templates of P2 (Thielaviopsis resistance
results obtained in 2009 and in 2010 on chicory and Sclerotinia resistance results obtained in 2009)
and P5 (chemical analysis results on spinach and orache).
2. What will be done : sending all templates (evaluation and characterisation) to P0 when data will be fully
available.
3. Bottlenecks : none
20
Persons involved at GEVES : Pascal Coquin (Valérie Cadot until November 2008), Jean-Michel
Retailleau (GEVES staff) and breeders for regeneration
Milestones & Deliverables :
Deliverable date
WP2
Status
GEVES
Partner
responsible
concluded
Done
P3
month 45
On going
P2, P8, P13
month 48
On going
(done for
P2)
month 48
On going
P0, P1, P2,
P3, P5, P6,
P7, P10, P13
P1, P2, P7,
P8, P13
month 48
On going
P0, P1, P2,
P3, P5, P7, P9
Milestone date
Status
GEVES
Partner
responsible
month 02
Done
month 06
Done
month 06
Year 1
D06; month 06
Year 4
Year 1
month 12
P3
P3
On going
P0, P1, P2,
P3, P5, P6,
P7, P8, P9,
P10, P13
On going
P0, P1, P2,
P3, P5, P6,
P7, P8, P9,
P10, P13
21
Year 2
To be done
P0, P1, P2,
P3, P4, P5,
P6, P7, P8,
P9, P10, P13
month 36
On going
P2, P8, P13
month 36
Done for
P2
month 36
On going
P0, P1, P2,
P3, P5, P6,
P7, P10, P13
P1, P2, P7,
P8, P13
month 36
On going
Year 4
See deliverables
month 24
Year 3
P0, P1, P2,
P3, P5, P7, P9
Activities
Reception of the chicory multiplications conducted in 2009 from the breeders network. About
regeneration, 12 accessions regenerated in 2009 have been sown in March 2010 in order to verify their
identity in comparison with the remaining seeds of their parent seeds. In the same time, in order to
continue the characterisation work, 61 accessions of chicories have been sown for the remaining
characters (bolting descriptors) : among those 61 accessions, 23 accessions have been partially
characterised in 2009 within an autumn trial (except bolting descriptors) and the 38 remaining
accessions have been already sown in 2009 in spring trial but it was not possible to have the bolting
descriptors due to a lack of low temperatures during spring 2009. In parallel, seeds of 30 accessions
have been sent to the breeders network for regeneration in January 2010.
2. What will be done : characterisation of the 61 accessions (see above) for bolting descriptors in a spring
trial sown in March 2010. Receiving the seeds regenerated of chicory in 2009. Sending 1 variety of
corn salad to one breeder for regeneration.
3. Bottlenecks :. none
Workpackage 3 : Evaluation
To coordinate and evaluate germplasm of the leafy vegetable crops for pest and disease resistance and
abiotic characters for a much more effective utilization of the genepools. With the outcome of this activity
subsequent breeding will result in more sustainable production methods and a better quality product.
Persons involved from GEVES: Pascal Coquin (Valérie Cadot until November 2008), Grimault Valérie
(GEVES-SNES),
From FNPE : Marc Benigni
22
Deliverable date
WP3
Status
GEVES
Partner
responsible
Done
P0, P1, P2,
P3, P4, P5,
P7, P8, P9,
P10, P12, P13
Not yet due
P2, P8, P12
Not yet due
P0, P1, P2,
P3, P4, P5,
P7, P8, P9,
P10, P12, P13
Status
GEVES
Partner
responsible
Done
Pall
Year 1
D16; month 12
Year 4
D19; month 45
D21; month 45
Milestone date
Evaluation data entered in a fixed format to be sent to
the database managers
Year 1
month 12
Year 2
month 18
Evaluations started
On going
Year 3
month 36
Year 4
See deliverables
Not yet due
P0, P1, P2,
P4, P5, P7,
P8, P9, P10,
P12, P13
P2, P8, P12
Activities
1. What has been done :
a) About Alternaria cichorii : choice of 20 new accessions has been done for the 2010 field test, i.e. to
show different levels of susceptibility according to the cultigroups of leaf chicory.
b) About Thielaviopsis basicola.: P2 (SNES = the central laboratory of GEVES) has finalised in
January 2010 the resistance tests for 41 accessions (of which 3 standards), i.e. the second wave
of this test began in autumn 2009 on 43 accessions (of which 3 standards). By the way, data
obtained on the whole program on such fungus have been sent to P0.
c) About Sclerotinia sclerotiorum : P2 (FNPE in contract with GEVES) finalised in growth chamber in
February 2010 the test by forcing roots inoculated by this fungus of 17 accessions sown in their
field in May 2009 for evaluation resistance against Sclerotinia sclerotiorum.
d) About Alternaria cichorii : in July 2010, 20 new accessions will be sown for assessment of the
resistance to Alternaria cichorii. Notations will be conducted in September after two inoculations in
August and in September following the way used in 2008 and in 2009 for the first 45 accessions
evaluated
e) About Thielaviopsis basicola : none.
f) About Sclerotinia sclerotiorum : FNPE will send report of the 2009/2010 test to P2 who will
integrate these data in the preformat files for evaluation and send it to P0.
23
Workpackage 5 : Co-ordination
General objectives: The general objective of this workpackage is the effective management of the
Persons involved at GEVES: Pascal Coquin (Valérie Cadot until November 2008)
Deliverable date
WP5
Status
GEVES
Partner
responsible
Done
P0/Pall
D28, month 03
Leafy Veg website is launched on internet and active
Done
P0
D29, month 06
Done
P2/P0
D30, month 11
Done
P2/P0
D31, month 14
Done
P0/Pall
D32, month 18
Done
P7/P0
D33, month 23
Done
P2/P0
D34, month 26
Done
P0/Pall
D35, month 30
Done
P9/P0
D36, month 35
Done
P3/P0
D37, month 38
Done
P0/Pall
D38, month 42
In progress
P8/P0
D 39, month 46
P4/P0
D40, month 48
Final annual progress report and workshop
P0/Pall
Milestone date
Status
GEVES
Partner
responsible
Detailed workplan of the project defined at the start -up
meeting of the project (time schedules for
regeneration, characterisation and evaluation will be
established)
Done
P0/Pall
Year 1
D27, month 01
Year 2
Year 3
Year 4
Year 1
month 01
24
month 01
Done
P0
month 5
Done
P2/P0
month 10
The second project meeting (first annual project
meeting) will be organised to discuss the progress and
discuss the first annual progress report
Done
P2/P0
month 13
First annual progress report will be finalised and
submitted to the European Commission
Done
P0/Pall
month 17
Done
P7/P0
month 22
Third project meeting (second annual project meeting)
will be organised to discuss the progress and discuss
the second annual progress report
Done
P5/P0
Second annual progress report submitted to the
European Commission
Done
P0/Pall
Done
P9/P0
Fourth project meeting (third annual project meeting)
Done
P3/P0
month 37
European Commission
Done
Pall
month 41
In progress
P8/P0
month 45
project organised.
P4/P0
month 48
P0/ Pall
Year 2
Year 3
month 25
month 29
month 34
Year 4
Activities
1. What has been done :
- P2 contributions to the global Leafy veg’s technical and financial report respectively in December
2009 (April 2010 for the related questions from the EU Commission on the technical report) and in
January 2010
2. What will be done :
- to prepare the 3rd WP coordinators meeting in Switzerland the 23rd June 2010
- to prepare the 4th and last annual project meeting in France, the 16-17 November 2010
3. Bottlenecks : None
25
P3: Leibniz Institute of Plant Genetics and Crop Plant Research
(IPK), Gatersleben, Germany
The group at IPK is engaged in three workpackages (WPs), WP1: Documentation, WP2: Regeneration
and Characterization and WP5: Co-ordination. For WP2 IPK is also WP leader.
General objectives:
 Development of crop databases for spinach, cichorium and minor leafy vegetables and updating the
existing international lactuca database.
databases.
Persons involved at IPK: Andreas Börner, Ulrike Lohwasser, Stefanie Thumm
Milestones & Deliverables:
Deliverable date
WP1
Status
all
partners
Partner
responsible
Year 3
D01; month 30
Year 4
D02; month 48
D03; month 48
D04; month 48
D05; month 48
Milestone date
collections donated
P0,P2,P3
Databases of the four LV crop groups developed and
on the Internet
P0,P2,P3
established
P0,P2,P3
P0,P2,P3
P0,P2,P3
Status
Partner
responsible
lettuce and spinach
Done
Pall
Year 1
month 03
26
month 03
Done
P0,P2,P3
month 06
Done
Pall
month 12
crop groups
Done
Pall
month 12
project meeting
Done
P0,P2,P3
delivered
Done
P0,P2,P3
month 36
nearly finalized
Done
P0,P2,P3
month 36
Done
P0,P2,P3
month 36
Done
P0,P2,P3
Done
P0,P2,P3
Ongoing
P0,P2,P3
month 42
Internet
month 42
realized
Ongoing
P0,P2,P3
Ongoing
Pall
Year 2
month 24
Year 3
month 36
Year 4
month 45
Remark: this task can not be completely fulfilled at the
official end of the project, as data of the fourth year will
not be available for processing in the databases. The
work has to be finalised after the project in the
framework of the ECP/GR working group on leafy
vegetables.
27
Activities
Request for partners and international passport data of minor leafy vegetables is finished. 56 gene banks
were contacted worldwide. Data have been only received from Italy, Romania, Greece, Poland, Sweden
and USA. International passport data collected: 88 accessions of Rheum, 229 accessions of Asparagus, 3
accessions of Valerianella, 257 accessions of Eruca, 30 accessions of Diplotaxis and 40 accessions of
Cynara. In total 2154 accessions of minor leafy vegetables have been collected. Harmonization of the
received passport data in respect of content and form is finished. All collected passport data are available
at the leafy vegetables website.
Start identifying gaps in the collections of the minor crops. Analyses were started for minor leafy
vegetables, and preliminary results for Asparagus were presented during the annual meeting in Cesena.
Because of insufficient available information about gene pool structures and distribution areas for the
majority of the minor leafy vegetables, it was decided to limit the gap analysis for minor leafy vegetables to
rocket salad (Eruca and Diplotaxis). Gap analysis started for the Eruca and Diplotaxis collection. 686
Eruca accessions, 148 Erucastrum accessions and 380 Diplotaxis accessions are in the database.
Analysis started with the description of the gene pool (primary, secondary, tertiary gene pool).
Identifying gaps in the Eruca and Diplotaxis collections is ongoing. For this purpose suitable literature will
be examined to identify to which gene pool the species belonging to and where are their distribution areas.
Analysing Eruca and Diplotaxis accessions in the database recording to their origin (areas/countries) and
type of material (cultivated, breeding material, landraces, wild). Comparison of the results obtained from
literature and analysis of the database. Results will be shown during the next meeting in June.
General objectives:
Persons involved at IPK: Stefanie Thumm
Status
Partner
responsible
concluded
Done
P3
Year 1
D06; month 06
Year 4
28
D07; month 45
Done
D08; month 45
Ongoing
P0, P1, P3,
P4, P7, P10,
P13
P1, P5, P7
D09; month 45
Done
P2, P8, P13
190 accessions of minor crops characterized
Ongoing
P0, P3, P5,
P9
Characterization data entered in a fixed format to be
sent to the database managers
Ongoing
P3
Ongoing
P0, P1, P2,
P3, P5, P6,
P7, P10, P13
Done
P0, P1, P5,
P7
Ongoing
P1, P2, P7,
P8, P13
Done
P0, P1, P2,
P3, P5, P7,
P9
D10; month 45
D11; month 45
D12; month 48
D13; month 48
D14; month 48
D15; month 48
Milestone date
Status
Partner
responsible
Done
P3
month 02
month 06
Done
P3
Done
P0, P1, P2,
P3, P5, P6,
P7, P8, P9,
P10, P13
Done
P0, P1, P2,
P3, P5, P6,
P7, P8, P9,
P10, P13
Done
P0, P1, P2,
P3, P4, P5,
P6, P7, P8,
P9, P10, P13
Year 1
month 06
month 12
Year 2
month 24
Year 3
29
Done
month 36
month 36
month 36
month 36
110 accessions of Minor crops characterized
month 36
month 36
month 36
month 36
Year 4
See deliverables
Done
P0, P1, P3,
P4, P7, P10,
P13
P1, P5, P7
Done
P2, P8, P13
Done
P0, P3, P5,
P9
Done
P0, P1, P2,
P3, P5, P6,
P7, P10, P13
Done
P0, P1, P5,
P7
Done
P1, P2, P7,
P8, P13
Done
P0, P1, P2,
P3, P5, P7,
P9
Activities
LACTUCA: 156 accessions sown on 9th of March 2010, 23 accessions sown again on 25th of March
2010 due to less germination rate (greenhouse, seeds sown into pots, approximately two weeks later
transferred into trays, still in the greenhouse on tables). Before planting out in the field on 20 th of April
2010 they were kept for one week in trays outside under a roof. All 156 accessions meant for
characterization, 66 of those for characterization and regeneration. 2009 harvest is completed.
ERUCA: 8 accession sown on 12th of April 2010 for characterization and regeneration and 28
accessions sown on 14th of April 2010 for characterization. 2009 harvest is completed.
VALERIANELLA: Harvest of 3 accessions from 2009 is finished. 16 accessions sown in August 2009
characterization is finished, harvest of 8 accessions is not finished yet. 4 accessions sown on 9th of
April 2010 for characterization.
LACTUCA, ERUCA: for this year’s material characterization is ongoing (this includes taking herbarium
samples, pictures, morphological description). The Lactuca for regeneration in the field will still get a
plastic tunnel roof before the first plants start to flower. Eruca will get several insecticide treatments
against Meligethes aeneus.
VALERIANELLA: Harvest of accession from 2009 will start, after seeds have dried to certain level seed
cleaning will start, germination tests will be conducted. Accessions from 2010 will be characterized in
summer.
30
3. Bottlenecks:
Workpackage 5: Co-ordination
General objectives: The general objective of this work package is the effective management of the
Persons involved at IPK: Andreas Börner, Ulrike Lohwasser, Stefanie Thumm
Deliverable date
WP5
Status
Partner
responsible
Done
P0/Pall
Year 1
D27, month 01
D28, month 03
Leafy Veg website is launched on internet and active
Done
P0
D29, month 06
Done
P2/P0
D30, month 11
Done
P1/P0
Done
Done
Done
P0/Pall
P7/P0
P5/P0
Done
Done
Done
P0/Pall
P9/P0
P3/P0
Final annual progress report and workshop
Done
P0/Pall
P8/P0
P4/P0
P0/Pall
Status
Partner
responsible
Done
P0/Pall
month 01
established)
month 01
Done
P0
Year 2
D31, month 14
D32, month 18
D33, month 23
Year 3
D34, month 26
D35, month 30
D36, month 35
Year 4
D37, month 38
D38, month 42
D 39, month 46
D40, month 48
Milestone date
Year 1
31
month 5
Done
P2/P0
Done
P1/P0
month 10
First annual progress report will be finalised and
submitted to the European Commission
Done
P0/Pall
Done
P7/P0
Done
P2/P0
month 25
Second annual progress report will be submitted to the
European Commission
Done
P0/Pall
month 29
Done
P9/P0
Done
P3/P0
month 34
will be organised to discuss the progress; this meeting
will also review the overall progress of the project (midterm review) in order to assess whether the following
set of milestones have been fulfilled by the project
Done
Pall
month 37
European Commission
month 41
P8/P0
P4/P0
month 45
project organised. A workshop presenting the results of
the project will be connected to this meeting to
disseminate information of the project to interesting
stake holders.
P0/ Pall
Year 2
month 13
month 17
month 22
Year 3
Year 4
month 48
Activities
New posters designed for the demonstration plots explaining and introducing the EU project.
Most accessions of Lactuca and Eruca for characterisation are grown at IPK on demonstration
plots open to visitors and employees.
32
Demonstration plots will be shown to visitors in the course of the State Horticultural Show
(Landesgartenschau) in Aschersleben in June.
Preparation for WP coordinator meeting, preparation for the open day of the IPK (12th of June
2010)
3. Bottlenecks: None
33
P4: Palacký University in Olomouc (Palacky), Czech Republic
The group at Palacký University is engaged in two workpackages (WPs), WP2: Regeneration &
characterization and WP3: Evaluation. Some activities are connected with tasks in the WP1:
Documentation and partner P4 develops activities leading to fulfillment of tasks in the WP5: Coordination
WP 3: Evaluation
Persons involved from Palacky: Aleš Lebeda, Irena Petrželová, Ivana Doležalová, Eva Křístková
Deliverables
(modified for
partner P4), date
Deliverables description
Status
Palacky
Partner
responsible
in progress
P4
in progress
P4
Year 4
D17; month 45
D21; month 45
30 accessions of lettuce evaluated for 20 races of
Bremia lactucae
Evaluation data for response of 30 accessions of
lettuce to 20 races of Bremia lactucae entered in a
fixed format to be sent to the database managers
D. Research
1. What has been done: Evaluation of response of 30 Lactuca sativa accessions to 15 races (isolates)
of Bremia lactucae.
2. What will be done: Evaluation of 30 lettuce accessions for response to 5 races (isolates) of Bremia
lactucae We are expecting to send evaluation data by the end of June 2010.
WP5: Co-ordination and dissemination
Persons involved at Palacky: Aleš Lebeda, Eva Křístková (participation at meetings, elaboration of
projects reports)
Elaboration and sending project year reports (technical, finnacial) for 2009 to co-ordinator
Elaboration of results obtained within the project GENRES LeafyVeg for IHC 2010 (Lisboa, Portugal):
Lebeda A., Křístková E., Doležalová I., Petrželová I., Melichaříková Z., 2009. Germplasm collections of
old lettuce cultivars and landraces for modern breeding. International Horticultural Congress, 22.27.8.2010, Lisboa, Portugal.
2. What will be done: what will be asked for
34
P5: Nordic Genetic Resource Center (NordGen)
The group at NGB is engaged in three workpackages (WPs), WP1: Documentation, WP2: Regeneration
and Characterization, WP3: Evaluation
NordGen have had the final analysis carried out and we have put the information into our databases - this
include both characterisation data and evaluation data from 2009. The data is submitted (or will be right
now) to the EU leafy veg project. We have also started regeneration on a few more accessions for seeds
to be harvested 2010. We have included some more accessions of Lactuca serriola in our collection, but
this has not been included in the project. Analysis of passport data on minor leafy vegetable crops is
started and the information will be given to the project.
35
P6: Institut National de Recherche Agronomique (INRA), Montfavet,
France
The group at INRA is engaged in the workpackage 2; WP2: Regeneration and Characterization for
regeneration of a part of the INRA lettuce collection
General objectives:
To regenerate and characterize parts of the lettuce genebank collection, for improving conservation and
utilization.
Persons involved at INRA: Brigitte Maisonneuve and Loic Jean (+ Christophe Giraud)
Milestone
date
Status
INRA
Partner
responsible
Year 1
month 12
Year 2
Regeneration and characterisation for lettuce started
Done
P3
month 24
Regeneration and characterisation for lettuce will be
continued
Done
P3
continued
Done
P3
In progress
P3
Year 3
month 36
Year 4
month 36
finished
Activities
a. Sowing (26 November) and transplantation in peat (1 December) of the 25 winter accessions
regenerated in 2009 to control the purity of the harvested seeds. Control of the type and the
homogeneity on 42 young plants per progeny (30 December).
b. Preparation of the third annual report for INRA: timesheets for 3 workers, financial report
c. Control of the type and the homogeneity for the 25 winter accessions (plastic tunnel planted (5
January) with 32 plants per progeny; photos and description of the accessions (March)
d. Sowing (22 February) and transplantation in pot (3 March) of the 70 accessions for regeneration in
2010. Control of the type and the homogeneity on the young plants.
e. Plantation of the 2 plants per genotype under an insect-proof plastic tunnel (31 March) for
regeneration
f.
Notation of some descriptors under plastic tunnel before opening of the head to improve the
bolting (in April). Weekly in April-May, take care of the plants (opening of the head, removing the
outer leaves)
g. Sowing (15 March) and transplantation in peat (19 March) of the 49 accessions of open field
varieties regenerated in 2009 to control the purity of the harvested seeds. Control of the type and
the homogeneity on 28 young plants per progeny (3 April)
h. Plantation of the 20 plants per progeny in a field (13 April), installation of a voile for wind protection
36
i.
j.
Send the safety duplication to Warwick (77 accessions regenerated in 2009, 10 May)
Control in a field of the type and the homogeneity for the 49 field accessions regenerated in 2009;
photos and description of the accessions (May)
a. Control the passport data send in 2007 to CGN for the 300 lettuce; inform R van Treuren if there
are some corrections.
b. Regeneration of the 70 accessions to obtain the 300 previous accessions. Send their safety
duplication to Warwick.
6. Bottlenecks:
None
37
P8:
Henry Doubleday Research Association (HDRA), UK
HDRA is engaged in three workpackages (WPs), WP2: Regeneration and Characterization, WP3:
Evaluation and WP4 Utilization & Marketing.
Persons involved at HDRA: Phil Sumption
Workpackage 4: Marketing
Deliverable date
Status
HDRA
Partner
responsible
15 accessions of Minor crops evaluated for utilisation
and marketing
Evaluation data entered in a fixed format to be sent to
the database managers
complete
P8
WP4
Year 4
D25; month 45
D26; month 45
Sent to WP
leader
Activities
Evaluation of minor crops completed. 10 valerianella accessions and 10 eruca accessions evaluated.
Evaluation data sent entered in a fixed format and sent to the WP leader.
Workpackage 5: Coordination
Activities
Talk given to Mercia Organic Producers. January 2010 ‘Rocket Science and other leafy vegetables’ by
Phil Sumption.
Article in The Organic Grower – January 2010. Lamb’s lettuce/corn salad pp36-37
38
P10: University of Bologna, Department of Agroenvironmental
Science and Technology (DiSTA-UNIBO), Bologna, Italy
WP 1: Documentation
Persons involved at UNIBO: L. Filippo D'Antuono, Manuela Agata manco
Deliverable date
Status
DiSTA-UNIBO
Partner
responsible
in course for
additional
chicory
accessions
Status
DiSTA-UNIBO
P0,P2,P3
Done
Pall
Done
Pall
P0,P2,P3
Passport data of most collections of the four
databases delivered
in course for
additional
chicory
accessions
P0,P2,P3
Databases with passport data for the four crop
groups nearly finalized
in course rro
additional
chicory
accessions
WP1
Year 3
D01; month 30
collections donated
Milestone date
Partner
responsible
Year 1
month 03
month 12
Start made selecting material used for the
evaluation activities, developing core collections if
needed for lettuce and spinach
Material selected for the evaluation activities of the
four crop groups
Year 2
month 24
Year 3
month 36
39
month 36
Part characterisation and evaluation data donated
and start made including these in the databases
done for
analytical data
of rocket salad
and sensory
data of rocket
salad and
chicory
P0,P2,P3
The passport data of additional 24 chicory accessions (not yet in Gene banks), that have been received by
Sativa seeds and services, and have been evaluated analytically and sensorially, were supplied to P2.
Seeds of these same accessions are being collected and will be supplied to P2 as well.
General objectives:
Persons involved at UNIBO: L. Filippo D'Antuono, Manuela Agata Manco, Federico Ferioli
Deliverable date
WP3
Status
DiSTAUNIBO
Partner
responsible
Year 1
D16; month 12
seeds of
Eruca
received
from P3
Year 4
D19; month 45
evaluation
started;
analytical
activities in
course
done for
rocket
salad (37
accessions)
D20; month 45
Milestone date
Status
DiSTAUNIBO
Done
Year 1
month 12
Partner
responsible
40
Year 2
done
month 18
Evaluations started
Year 3
month 36
month 36
evaluation
started;
analytical
activities in
course
done for
rocket
salad (37
accessions)
Activities
Eruca and Diplotaxis evaluation for glucosinolates
The final analytical evaluation of rocket salad glucosinolates have been finished during the period. The
data were send to WP leader according to the standard format
Chicory evaluation for sesquiterpene lactones
A total of 102 accession were considered; 24 were received from Sativa seeds and services and the
remaining from Geves. 70 accessions belonged to different types of C. inthybus and 32 to C. endivia.
Seeds were sown in trays on July 25 2010 and transplanted in the field on August 17. harvest started on
October 27 and went on until Christmas, according to precocity. Five late Treviso types were seriously
damaged by an early frost, and were not harvested.
The harvested material was trimmed and the edible part separated from waste. On both parts, water
content was determined, and both were freeze-dried.
8 additional eight witloof accessions (the edible product were received from P2 in March 2010, and were
prepared in the same way as the others.
Total number of accession submitted to analysis was therefore 105.
Until now the activities done were the following:
- Preliminary purification trials by means of TLc and direct and reverse phase SPE. Chosen direct phase
SPE.
- Preliminary extraction trials with different solvent mixtures: chosen methanol:water, 4:1 + 2% formic acid.
- sample lyophilisation
- sample extraction
- purification of free lactones
- recovery of bound lactones by cellulase treatment
- purification of total lactones
- start HPLC injections
41
Workpackage 4: Marketing
Persons involved at DiSTA-UNIBO: L. Filippo D'Antuono, Manulea Agata Manco
Deliverable date
WP4
Status
DiSTAUNIBO
Partner
responsible
Year 1
Done
D22; month 12
Seeds of the selected material of the three crop groups
Year 4
D24; month 45
125 accessions of Chicory evaluated for utilisation and
marketing
D25; month 45
15 accessions of Minor crops evaluated for utilisation
and marketing
Milestone date
sensory
evaluation
of 28
endives
done
P8/P12
sensory
evaluation
of 37 rocket
salads
done
Status
P9/P10
Done
P2/P3/P7/P8/P9
/
P10/P12/P13 +
companies
Done
P7/P8/P9/
P10/P12/P13
sensory
evaluation
of 28
endives
done
sensory
evaluation
of 37 rocket
salads
done
P8/P12
Partner
responsible
Year 1
month 12
Material selected for utilization and marketing
Year 2
month 18
Activities on utilisation and marketing started
Year 3
month 36
month 36
75 accessions of chicory evaluated for utilisation and
marketing
10 accessions of minor crops evaluated for utilisation
and marketing
P9/P10
During the autumn 2010, 28 endive accessions have been evaluated by means of sensory analysis.
Preliminary a was selected and trained for the evaluation of the three specific characters: bitter,
herbaceous, astringent, that were judged as significant for the evaluation of the available material.
42
The evaluation sessions were carried out according to the availability of material, but never including more
than 6 samples per days, to avoid tiring and carry over effects
Workpackage 5: Coordination
Persons involved at DiSTA-UNIBO: L. Filippo D'Antuono, Manuela Agata Manco, Federico Ferioli
Deliverable date
WP5
Status
DiSTAUNIBO
Partner
responsible
attended
P0/Pall
attendance
was not
possible for
family
reasons
P1/P0
attended
P2/P0
Year 1
D27, month 01
D30, month 11
Year 2
D33, month 23
Year 3
D34, month 26
D35, month 30
D36, month 35
organised
and hosted
in Cesena
P0/Pall
P9/P0
P3/P0
Year 4
planned to
be attended
P4/P0
Status
DiSTAUNIBO
Partner
responsible
established)
done /
attended
P0/Pall
not
attended
for family
reasons
P1/P0
D 39, month 46
Milestone date
Year 1
month 01
month 10
43
Year 2
month 22
attended
P2/P0
will be organised to discuss the progress; this meeting
will also review the overall progress of the project (midterm review) in order to assess whether the following
set of milestones have been fulfilled by the project
organised
and hosted
in Cesena
P3/P0
planned to
attend
P4/P0
Year 3
month 34
Year 4
month 45
project organised.
Activities
Third year scientific and financial reports were regularly delivered, including some further clarifications.
The UNIBO team organised and hosted the fourth Consortium meeting in Cesena, Italy, on 17-18 November 2009
44
P10: Agricultural Institute of Slovenia, KIS, Ljubljana, Slovenia
No information yet received
45
P11: ProSpecieRara, Aarau, Switserland
General objectives: evaluation of marketability of leaf chicory and endive
Deliverable date
WP3
Status
Partner
responsible
Ready to
deliver
P8
Evaluation of marketability of selected varieties
Status
Partner
responsible
Planning of
degustation;
varieties still
growing
P8
Year 4
Dec 2009 – May
2010
Milestone date
Year 4
June 2010 –
September 2010
Selected varietes of endive and leaf chicory will be
tested for their sensory value
Workpackage 2: Characterization
General objectives: characterization of selected leaf chicory and endive varieties
Deliverable date
WP2
Status
Partner
responsible
P8
Time of bolting
and flower
coloration
missing, wil be
evaluated this
summer 2010
Status
-
-
-
Year 4
Dec 2009 – May
2010
Milestone date
Year 4
Characterisation of 6 endive and 45 leaf chicory
varieties
Partner
responsible
46
Activities
1.
2.
Missing characterisation/evaluation/marketability data for 2007/2008 (11 leaf chicory, 12 endive; 8
leaf chicory, 28 endivia) demanded from the partners. Composition of the data is ongoing.
Due to late planting time 2009 (6 endive, 45 leaf chicory) from GEVES,CHERAC, PSR, Samen
Mauser the varieties bolted only in 2nd year. These Data is still missing. Additionally some of the
varieties did not get through hibernation and had to be resawn (6 endive, 13 leaf chicory).
There will be organized a degustation event of the resawn varieties. The missing data of bolting
time and flower coloration will be evaluated.

GARLIC AND HEALTH PROJECT

Transcription

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