GARLIC AND HEALTH PROJECT
Transcription
GARLIC AND HEALTH PROJECT
1 Leafy Vegetables germplasm: stimulating use Contract number: AGRI-2006-0262 Agreement number: 001 AGRI GENRES 870/2004 E-mail report December 2009 – May 2010 (E-report 09) Cesena 2009 2 General information 1. 2. 3. 4. The technical and financial reports of the third year have been submitted to the Commission by March. We received comments on the technical report in April and have submitted our reply to the Commission by early May. Hopefully the Commission will accept the technical report soon, as after the acceptance of this report the evaluation of the financial report will start. The annual project meeting in November 2009 was hosted by UNIBO (Cesena, Italy). We would like to thank Filippo D’Antuono and his students very much for their kind hospitality in making this meeting to a success. In June the annual WP coordinator meeting is planned at ProspecieRara (Aarau, Switzerland). As a coordinator I would like to stress again that when you are disseminate material (scientific, professional, web publications, articles in newpapers etc) you need to acknowledge the EU-GENRES programme in this article via the following wording (see also FAQ 11); "Action AGRI-2006-0262, Leafy Vegetables – stimulating use, receives financial support from the European Commission, Directorate-General for Agriculture and Rural Development, under Council Regulation (EC) No 870/2004". 3 List of milestones & deliverables (only to be updated by the WP leaders) Overview of deliverables Deliverable number Planned completion Status Description Achieved On going* D01 30 Passport data of the four leafy vegetable (LV) collections donated x D02 48 Databases of the four LV crop groups developed and on the Internet x D03 48 Evaluation and characterization results in fixed formats linked to the databases x D04 48 Safety duplication system of the LV collections established x D05 48 Gaps in the four LV crop collection groups identified x D06 06 Minimum descriptors for the minor leafy vegetables concluded D07 45 800 accessions of lettuce characterized x D08 45 110 accessions of spinach characterized x D09 45 180 accessions of chicory characterized x D10 45 D11 45 Characterization data entered in a fixed format to be sent to the database managers x D12 48 930 accessions of lettuce regenerated x D13 48 50 accessions of spinach regenerated x 190 accessions of minor crops characterized x x Not started yet* 4 D14 48 200 accessions of chicory regenerated x D15 48 70 accessions of minor crops regenerated x D16 12 Seeds of the selected material of the four crop groups sent to the partners involved D17 45 115 accessions of lettuce evaluated x D18 45 320 accessions of spinach evaluated x D19 45 265 accessions of chicory evaluated x D20 45 49 accessions of minor crops evaluated x D21 45 Evaluation data entered in a fixed format to be sent to the database managers x D22 12 Seeds of the selected material of the three crop groups sent to the partners involved D23 45 40 accessions of lettuce evaluated for utilisation and marketing D24 45 125 accessions of chicory evaluated for utilisation and marketing x D25 45 15 accessions of minor crops evaluated for utilisation and marketing x D26 45 Evaluation data entered in a fixed format to be sent to the database managers. 01 Start-up project meeting x D28 03 Leafy Veg website is launched on internet and active. x D29 06 First WP coordinators meeting x D30 11 First annual project meeting x D31 14 First annual progress report D27 x x x x x 5 D32 18 Second WP coordinators meeting x D33 23 Second annual project meeting x D34 26 Second annual progress report x D35 30 Third WP coordinators meeting x D36 35 Third annual project meeting x D37 38 Third annual progress report x D38 42 Fourth WP coordinators meeting D39 46 Final annual project meeting x D40 48 Final annual progress report x x 6 Overview of milestones Milestone number Planned completion Status Description Achieved (month) Start made selecting material used for the evaluation activities, developing core collections if needed for lettuce and spinach Start made requesting passport data for the spinach, chicory and minor vegetable databases and lacking data for the Lactuca database (ILDB) Material selected for regeneration and characterization of the four crop groups On going* M01 03 x M02 03 M03 06 M04 12 Material selected for the evaluation activities of the four crop groups x M05 12 Inventory of system of safety duplication of the collections made and discussed during the second project meeting x M06 24 Passport data of most collections of the four databases delivered x M07 36 Databases with passport data for the four crop groups nearly finalized x M08 36 Part characterisation and evaluation data donated and start made including these in the databases x M09 36 Safety duplication of part of the collections realized x M10 36 Start made identifying gaps in the collections x M11 42 Start made with making the databases available via the Internet x M12 42 Safety duplication of collections inside the project realized x M13 45 Most characterisation and evaluation data donated for inclusion in the databases x M14 02 Start made developing minimum descriptors for the minor leafy vegetables x x x Not started yet* 7 M15 06 Material for regeneration and characterization of the four crops selected x M16 06 Regeneration of material of some crops started pending the growing season x M17 12 Regeneration and characterisation for all crops started x M18 24 Regeneration, characterisation of germplasm of the four collections will be continued x M19 36 500 accessions of lettuce characterized x M20 36 70 accessions of spinach characterized x M21 36 110 accessions of chicory characterized x M22 36 110 accessions of Minor crops characterized x M23 36 600 accessions of lettuce regenerated x M24 36 30 accessions of spinach regenerated x M25 36 120 accessions of chicory regenerated x M26 36 40 accessions of minor crops regenerated x M27 12 Material selected for the evaluation activities of the four crop groups (see WP1) x M28 18 M29 36 70 accessions of lettuce evaluated x M30 36 210 accessions of spinach evaluated x M31 36 170 accessions of chicory evaluated x M32 36 30 accessions of minor crops evaluated Evaluations started x x 8 M33 12 Material selected for utilization and marketing x M34 18 Activities on utilisation and marketing started x M35 36 25 accessions of lettuce evaluated for utilisation and marketing x M36 36 75 accessions of chicory evaluated for utilisation and marketing x M37 36 10 accessions of minor crops evaluated for utilisation and marketing x M38 45 40 accessions of lettuce evaluated for utilisation and marketing x M39 45 125 accessions of chicory evaluated for utilisation and marketing x M40 45 15 accessions of minor crops evaluated for utilisation and marketing x M41 01 M42 01 M43 05 M44 10 M45 13 M46 17 M47 22 Detailed workplan of the project defined at the start -up meeting of the project (time schedules for regeneration, characterisation and evaluation will be established) The project website will be developed First WP coordinators meeting organised x x x The second project meeting (first annual project meeting) will be organised to discuss the progress and discuss the first annual progress report x First annual progress report will be finalised and submitted to the European Commission x Second WP coordinators meeting organised Third project meeting (second annual project meeting) will be organised to discuss the progress and discuss the second annual progress report x x 9 M48 25 M49 29 M50 34 M51 37 M52 41 M53 45 M54 48 Second annual progress report will be submitted to the European Commission Third WP coordinators meeting organised Fourth project meeting (third annual project meeting) will be organised to discuss the progress; this meeting will also review the overall progress of the project (mid-term review) in order to assess whether the following set of milestones have been fulfilled by the project Third annual progress report including a mid-term review of the coordinator will be submitted to the European Commission Fourth WP coordinators meeting organised x x x x x Final project meeting to discuss the progress report of the fourth year and the final overall project report of the project organised. x Fourth annual progress report and the final overall report will be submitted to the European Commission x 10 P0: Centre Netherlands for Genetic Resources (CGN), Wageningen, the The group at CGN is engaged in four workpackages (WPs), WP1: Documentation, WP2: Regeneration and Characterization, WP3: Evaluation and WP5: Co-ordination. For WP1 and WP5 CGN is also WP leader. Workpackage 1: Documentation General objectives: Development of crop databases for spinach, Cichorium and minor leafy vegetables and updating the existing International Lactuca database. Prioritize accessions for regeneration, characterization and evaluation. If necessary, special selections or core collections will be made for the evaluations of the different crops with the help of these databases. Link results of the characterization and evaluation activities of the project to the respective databases. Establish a functional system of safety duplication of the leafy vegetable crops in cooperation with the ECP/GR Leafy Vegetables Working Group. Identify gaps in the collections also in cooperation with the ECP/GR Leafy Vegetables Working Group, to broaden the collections. Persons involved at CGN: Rob van Treuren, Frank Menting Milestones & Deliverables: Deliverable date Deliverable description Status CGN Partner responsible Passport data of the four leafy vegetable (LV) collections donated Done P0,P2,P3 Databases of the four crop groups developed and on the Internet Evaluation and characterization results in fixed formats linked to the databases Safety duplication system of the LV collections established Gaps in the four LV crop collection groups identified Milestone description Done P0,P2,P3 Ongoing Pall Ongoing Pall Ongoing Status CGN P0,P2,P3 Partner responsible WP1 Year 1 Year 2 Year 3 D01; month 30 Year 4 D02; month 48 D03; month 48 D04; month 48 D05; month 48 Milestone date Year 1 none none 11 Start made selecting material used for the evaluation activities, developing core collections if needed for lettuce and spinach Done Pall Done P0,P2,P3 month 03 Start made requesting passport data for the spinach, chicory and minor vegetable databases and lacking data for the Lactuca database (ILDB) Material selected for regeneration and characterization of the four crop groups Done Pall month 06 Done Pall month 12 Material selected for the evaluation activities of the four crop groups Inventory of system of safety duplication of the collections made and discussed during the second project meeting Done P0,P2,P3 month 12 Done P0,P2,P3 Done P0,P2,P3 Done P0,P2,P3 Done P0,P2,P3 Start made identifying gaps in the collections Done P0,P2,P3 Start made with making the databases available via the Internet Done P0,P2,P3 month 42 Safety duplication of collections inside the project realized Ongoing Pall month 42 Most characterisation and evaluation data donated for inclusion in the databases Ongoing Pall month 45 month 03 Year 2 month 24 Passport data of most collections of the four databases delivered Year 3 month 36 Databases with passport data for the four crop groups nearly finalized month 36 Part characterisation and evaluation data donated and start made including these in the databases month 36 Safety duplication of part of the collections realized month 36 Year 4 12 Activities 1. What has been done: Extension and updating of the databases. Including new project data in the databases. New functionalities for easier access to project data have been included in the databases. Gap analysis for lettuce and spinach was carried out. 2. What will be done: Continuation of including characterization and evaluation data in the databases. Preparation of a draft paper about the new databases and gap analysis in cooperation with GEVES and IPK. 3. Bottlenecks: None. Workpackage 2: Regeneration and characterization General objectives: To regenerate and characterize parts of partner’s genebank collections, for improving conservation and utilization. High priority will be given to material to be used in the evaluations, because the total numbers of accessions included in all collections needing regeneration and characterization are far too high to be handled in the framework of this project. Persons involved at CGN: Liesbeth de Groot, Rob van Treuren Milestones & Deliverables Deliverable date WP2 Deliverable description Status CGN Partner responsible done P3 D06; month 06 Minimum descriptors for the minor leafy vegetables concluded Year 2 None Year 3 None Milestone date Milestone description Status CGN Partner responsible Start made developing minimum descriptors for the minor leafy vegetables done P3 month 02 month 06 Material for regeneration and characterization of the four crops selected (see also WP1) done P3 Regeneration of material of some crops started pending the growing season done P0, P1, P2, P3, P5, P6, P7, P8, P9, P10, P13 Regeneration and characterisation for all crops started done P0, P1, P2, P3, P5, P6, P7, P8, P9, P10, P13 Year 1 Year 1 month 06 month 12 13 Year 2 month 24 Regeneration, characterisation of germplasm of the four collections will be continued Done P0, P1, P2, P3, P4, P5, P6, P7, P8, P9, P10, P13 P0, P1, P3, P4, P7, P10, P13 Year 3 month 36 60 accessions of lettuce characterized month 36 5 accessions of rucola characterized Done (161 + 284 acc.). 320 planned for 2010 Done (17 acc.) 60 accessions of lettuce regenerated Done (70 acc.) P0, P1, P2, P3, P5, P6, P7, P10, P13 month 36 15 accessions of spinach regenerated Done (16 acc.) P0, P1, P5, P7 month 36 5 accessions of rucola regenerated Done (11 acc.) P0, P1, P2, P3, P5, P7, P9 month 36 P0, P3, P5, P9 Activties 1. What has been done: 17 rucola accessions were characterized in an experimental field trial, and the data were included in the database. 16 accessions of spinach were regenerated. 2. What will be done: characterization of 320 lettuce accessions. 3. Bottlenecks: None. Workpackage 3: Evaluation General objectives: To evaluate germplasm of the leafy vegetable crops for pest and disease resistance and abiotic characters for a much more effective utilization of the genepools. With the outcome of this activity subsequent breeding will result in more sustainable production methods and a better quality product. Persons involved from CGN: Liesbeth de Groot and Rob van Treuren 14 Milestones & Deliverables Deliverable date Deliverable description Status CGN Seeds of the selected material of the four crop groups sent to the partners involved Done D16; month 12 Year 2 None Milestone date Milestone description Status CGN Material selected for the evaluation activities of the four crop groups (see WP1) Done Partner responsible WP3 Year 1 Partner responsible Year 1 month 12 Year 2 month 18 Evaluations started Done P0, P1, P2, P4, P5, P7, P8, P9, P10, P12, P13 Ongoing P0, P5 Year 3 month 36 250 accessions of spinach evaluated Activities 1. What has been done: Evaluation of 263 spinach accessions for 2 Peronospora pathotypes (Pfs1 & Pfs4). Data were included in the database. 2. What will be done: Evaluation of 263 spinach accessions for 3 additional Peronospora pathotypes (Pfs3, Pfs6, Pfs10). 3. Bottlenecks: None. Workpackage 5: Co-ordination General objectives: The general objective of this workpackage is the effective management of the project. Management of the project will take place on three levels: on the integral project level, on the WP level and on the crop task level. Persons involved at CGN: Chris Kik 15 Milestones & Deliverables Deliverable date WP5 Deliverable description Status CGN Year 2 done done Year 3 done Year 1 Partner responsible Year 4 done P0/Pall D37, month 38 Third annual progress report D38, month 42 Fourth WP coordinators meeting P8/P0 D 39, month 46 Final annual project meeting P4/P0 D40, month 48 Final annual progress report P0/Pall Milestone date Milestone description Status CGN Year 1 done Year 2 done Year 3 done Partner responsible Year 4 done Pall month 37 Third annual progress report including a mid-term review of the coordinator will be submitted to the European Commission month 41 Fourth WP coordinators meeting organised ongoing P8/P0 P4/P0 month 45 Final project meeting to discuss the progress report of the fourth year and the final overall project report of the project organised. month 48 Fourth annual progress report and the final overall report will be submitted to the European Commission P0/ Pall Activities 1. What has been done: a. Annual meeting was perfectly organized by Filippo D‘Antuono and his students in Cesena in November 2009. All partners presented the work carried out in the reporting period and an overview and a discussion per WP was given by the various WP leaders. The project proved to be well on track. Third annual technical and financial progress report was written in January-March 2010 and was submitted to the EU by March. The reaction of the EU was received in April and a reply was written by medio May. 16 2. What will be done: a. Organizing the fourth annual WP meeting together with Prospecie Rara on June 23 in Aarau, Switzerland b. Revision/updating of the third annual technical and financial reports. 3. Bottlenecks: None 17 P1: Warwick HRI, University of Warwick, UK (WHRI) No information yet received 18 P2: Groupe d’Etudes et de contrôle des Variétés et des Semences (GEVES), Brion, France The group at GEVES is engaged in four workpackages (WPs), WP1: Documentation, WP2: Regeneration and Characterization, WP3: Evaluation and WP5: Co-ordination. For WP3, GEVES is also WP leader. Workpackage 1: Documentation General objectives : Development of crop databases for Cichorium Prioritize accessions for regeneration, characterization and evaluation. If necessary, special selections or core collections will be made for the evaluations of the different crops with the help of these databases. Link results of the characterization and evaluation activities of the project to the respective databases. Establish a functional system of safety duplication of the leafy vegetable crops in cooperation with the ECP/GR Leafy Vegetables Working Group. Identify gaps in the collections also in cooperation with the ECP/GR Leafy Vegetables Working Group, to broaden the collections. Persons involved at GEVES : Pascal Coquin (Valérie Cadot until November 2008) Milestones & Deliverables : Deliverable date Deliverable description WP1 Year 1 Year 2 Year 3 None None Passport data of the four leafy vegetable (LV) collections donated Status GEVES Partner responsible Done P0,P2,P3 Done P0,P2,P3 On going P0,P2,P3 On going P0,P2,P3 Year 4 month 48 month 48 month 48 Databases of the four LV crop groups developed and on the Internet Evaluation and characterization results in fixed formats linked to the databases Safety duplication system of the LV collections established month 48 Gaps in the four LV crop collection groups identified On going P0,P2,P3 Milestone date Milestone description Status GEVES Partner responsible month 03 Start made selecting material used for the evaluation activities, developing core collections if needed for lettuce and spinach Done Pall month 03 Start made requesting passport data for the spinach, chicory and minor vegetable databases and lacking data for the Lactuca database (ILDB) Done P0,P2,P3 Year 1 19 month 06 Material selected for regeneration and characterization of the four crop groups Done Pall month 12 Material selected for the evaluation activities of the four crop groups Done Pall month 12 Inventory of system of safety duplication of the collections made and discussed during the second project meeting Done P0,P2,P3 Passport data of most collections of the four databases delivered Done P0,P2,P3 month 36 Databases with passport data for the four crop groups nearly finalized Done P0,P2,P3 month 36 Part characterisation and evaluation data donated and start made including these in the databases On going P0,P2,P3 month 36 Safety duplication of part of the collections realized On going P0,P2,P3 month 36 Start made identifying gaps in the collections On going P0,P2,P3 month 42 Start made with making the databases available via the Internet On going P0,P2,P3 month 42 Safety duplication of collections inside the project realized On going P0,P2,P3 month 45 Most characterisation and evaluation data donated for inclusion in the databases On going Pall Year 2 month 24 Year 3 Year 4 Activities 1. What has been done: validation and sending of evaluation templates of P2 (Thielaviopsis resistance results obtained in 2009 and in 2010 on chicory and Sclerotinia resistance results obtained in 2009) and P5 (chemical analysis results on spinach and orache). 2. What will be done : sending all templates (evaluation and characterisation) to P0 when data will be fully available. 3. Bottlenecks : none 20 Workpackage 2: Regeneration and characterization General objectives : To regenerate and characterize parts of partner’s genebank collections, for improving conservation and utilization. High priority will be given to material to be used in the evaluations, because the total numbers of accessions included in all collections needing regeneration and characterization are far too high to be handled in the framework of this project. Persons involved at GEVES : Pascal Coquin (Valérie Cadot until November 2008), Jean-Michel Retailleau (GEVES staff) and breeders for regeneration Milestones & Deliverables : Deliverable date WP2 Deliverable description Status GEVES Partner responsible Minimum descriptors for the minor leafy vegetables concluded Done P3 month 45 180 accessions of chicory characterized On going P2, P8, P13 month 48 930 accessions of lettuce regenerated On going (done for P2) month 48 200 accessions of chicory regenerated On going P0, P1, P2, P3, P5, P6, P7, P10, P13 P1, P2, P7, P8, P13 month 48 70 accessions of minor crops regenerated On going P0, P1, P2, P3, P5, P7, P9 Milestone date Milestone description Status GEVES Partner responsible month 02 Start made developing minimum descriptors for the minor leafy vegetables Done month 06 Material for regeneration and characterization of the four crops selected (see also WP1) Done month 06 Regeneration of material of some crops started pending the growing season Year 1 D06; month 06 Year 4 Year 1 month 12 Regeneration and characterisation for all crops started P3 P3 On going P0, P1, P2, P3, P5, P6, P7, P8, P9, P10, P13 On going P0, P1, P2, P3, P5, P6, P7, P8, P9, P10, P13 21 Year 2 Regeneration, characterisation of germplasm of the four collections will be continued To be done P0, P1, P2, P3, P4, P5, P6, P7, P8, P9, P10, P13 month 36 110 accessions of chicory characterized On going P2, P8, P13 month 36 600 accessions of lettuce regenerated Done for P2 month 36 120 accessions of chicory regenerated On going P0, P1, P2, P3, P5, P6, P7, P10, P13 P1, P2, P7, P8, P13 month 36 40 accessions of minor crops regenerated On going Year 4 See deliverables month 24 Year 3 P0, P1, P2, P3, P5, P7, P9 Activities 1. What has been done: Reception of the chicory multiplications conducted in 2009 from the breeders network. About regeneration, 12 accessions regenerated in 2009 have been sown in March 2010 in order to verify their identity in comparison with the remaining seeds of their parent seeds. In the same time, in order to continue the characterisation work, 61 accessions of chicories have been sown for the remaining characters (bolting descriptors) : among those 61 accessions, 23 accessions have been partially characterised in 2009 within an autumn trial (except bolting descriptors) and the 38 remaining accessions have been already sown in 2009 in spring trial but it was not possible to have the bolting descriptors due to a lack of low temperatures during spring 2009. In parallel, seeds of 30 accessions have been sent to the breeders network for regeneration in January 2010. 2. What will be done : characterisation of the 61 accessions (see above) for bolting descriptors in a spring trial sown in March 2010. Receiving the seeds regenerated of chicory in 2009. Sending 1 variety of corn salad to one breeder for regeneration. 3. Bottlenecks :. none Workpackage 3 : Evaluation General objectives : To coordinate and evaluate germplasm of the leafy vegetable crops for pest and disease resistance and abiotic characters for a much more effective utilization of the genepools. With the outcome of this activity subsequent breeding will result in more sustainable production methods and a better quality product. Persons involved from GEVES: Pascal Coquin (Valérie Cadot until November 2008), Grimault Valérie (GEVES-SNES), From FNPE : Marc Benigni 22 Deliverable date WP3 Status GEVES Partner responsible Seeds of the selected material of the four crop groups sent to the partners involved Done P0, P1, P2, P3, P4, P5, P7, P8, P9, P10, P12, P13 265 accessions of chicory evaluated Not yet due P2, P8, P12 Not yet due P0, P1, P2, P3, P4, P5, P7, P8, P9, P10, P12, P13 Status GEVES Partner responsible Done Pall Deliverable description Year 1 D16; month 12 Year 4 D19; month 45 D21; month 45 Milestone date Evaluation data entered in a fixed format to be sent to the database managers Milestone description Year 1 month 12 Material selected for the evaluation activities of the four crop groups (see WP1) Year 2 month 18 Evaluations started On going Year 3 month 36 170 accessions of chicory evaluated Year 4 See deliverables Not yet due P0, P1, P2, P4, P5, P7, P8, P9, P10, P12, P13 P2, P8, P12 Activities 1. What has been done : a) About Alternaria cichorii : choice of 20 new accessions has been done for the 2010 field test, i.e. to show different levels of susceptibility according to the cultigroups of leaf chicory. b) About Thielaviopsis basicola.: P2 (SNES = the central laboratory of GEVES) has finalised in January 2010 the resistance tests for 41 accessions (of which 3 standards), i.e. the second wave of this test began in autumn 2009 on 43 accessions (of which 3 standards). By the way, data obtained on the whole program on such fungus have been sent to P0. c) About Sclerotinia sclerotiorum : P2 (FNPE in contract with GEVES) finalised in growth chamber in February 2010 the test by forcing roots inoculated by this fungus of 17 accessions sown in their field in May 2009 for evaluation resistance against Sclerotinia sclerotiorum. 2. What will be done: d) About Alternaria cichorii : in July 2010, 20 new accessions will be sown for assessment of the resistance to Alternaria cichorii. Notations will be conducted in September after two inoculations in August and in September following the way used in 2008 and in 2009 for the first 45 accessions evaluated e) About Thielaviopsis basicola : none. f) About Sclerotinia sclerotiorum : FNPE will send report of the 2009/2010 test to P2 who will integrate these data in the preformat files for evaluation and send it to P0. 3. Bottlenecks: None. 23 Workpackage 5 : Co-ordination General objectives: The general objective of this workpackage is the effective management of the project. Management of the project will take place on three levels: on the integral project level, on the WP level and on the crop task level. Persons involved at GEVES: Pascal Coquin (Valérie Cadot until November 2008) Milestones & Deliverables Deliverable date WP5 Deliverable description Status GEVES Partner responsible Start-up project meeting Done P0/Pall D28, month 03 Leafy Veg website is launched on internet and active Done P0 D29, month 06 First WP coordinators meeting Done P2/P0 D30, month 11 First annual project meeting Done P2/P0 D31, month 14 First annual progress report Done P0/Pall D32, month 18 Second WP coordinators meeting Done P7/P0 D33, month 23 Second annual project meeting Done P2/P0 D34, month 26 Second annual progress report Done P0/Pall D35, month 30 Third WP coordinators meeting Done P9/P0 D36, month 35 Third annual project meeting Done P3/P0 D37, month 38 Third annual progress report Done P0/Pall D38, month 42 Fourth WP coordinators meeting In progress P8/P0 D 39, month 46 Final annual project meeting P4/P0 D40, month 48 Final annual progress report and workshop P0/Pall Milestone date Milestone description Status GEVES Partner responsible Detailed workplan of the project defined at the start -up meeting of the project (time schedules for regeneration, characterisation and evaluation will be established) Done P0/Pall Year 1 D27, month 01 Year 2 Year 3 Year 4 Year 1 month 01 24 month 01 The project website will be developed Done P0 month 5 First WP coordinators meeting organised Done P2/P0 month 10 The second project meeting (first annual project meeting) will be organised to discuss the progress and discuss the first annual progress report Done P2/P0 month 13 First annual progress report will be finalised and submitted to the European Commission Done P0/Pall month 17 Second WP coordinators meeting organised Done P7/P0 month 22 Third project meeting (second annual project meeting) will be organised to discuss the progress and discuss the second annual progress report Done P5/P0 Second annual progress report submitted to the European Commission Done P0/Pall Done P9/P0 Fourth project meeting (third annual project meeting) Done P3/P0 month 37 Third annual progress report including a mid-term review of the coordinator will be submitted to the European Commission Done Pall month 41 Fourth WP coordinators meeting organised In progress P8/P0 month 45 Final project meeting to discuss the progress report of the fourth year and the final overall project report of the project organised. P4/P0 month 48 Fourth annual progress report and the final overall report will be submitted to the European Commission P0/ Pall Year 2 Year 3 month 25 month 29 month 34 Third WP coordinators meeting organised Year 4 Activities 1. What has been done : - P2 contributions to the global Leafy veg’s technical and financial report respectively in December 2009 (April 2010 for the related questions from the EU Commission on the technical report) and in January 2010 2. What will be done : - to prepare the 3rd WP coordinators meeting in Switzerland the 23rd June 2010 - to prepare the 4th and last annual project meeting in France, the 16-17 November 2010 3. Bottlenecks : None 25 P3: Leibniz Institute of Plant Genetics and Crop Plant Research (IPK), Gatersleben, Germany The group at IPK is engaged in three workpackages (WPs), WP1: Documentation, WP2: Regeneration and Characterization and WP5: Co-ordination. For WP2 IPK is also WP leader. Workpackage 1: Documentation General objectives: Development of crop databases for spinach, cichorium and minor leafy vegetables and updating the existing international lactuca database. Prioritize accessions for regeneration, characterization and evaluation. If necessary, special selections or core collections will be made for the evaluations of the different crops with the help of these databases. Link results of the characterization and evaluation activities of the project to the respective databases. Establish a functional system of safety duplication of the leafy vegetable crops in cooperation with the ECP/GR Leafy Vegetables Working Group. Identify gaps in the collections also in cooperation with the ECP/GR Leafy Vegetables Working Group, to broaden the collections. Persons involved at IPK: Andreas Börner, Ulrike Lohwasser, Stefanie Thumm Milestones & Deliverables: Deliverable date Deliverable description WP1 Status all partners Partner responsible Year 3 D01; month 30 Year 4 D02; month 48 D03; month 48 D04; month 48 D05; month 48 Milestone date Passport data of the four leafy vegetable (LV) collections donated P0,P2,P3 Databases of the four LV crop groups developed and on the Internet Evaluation and characterization results in fixed formats linked to the databases P0,P2,P3 Safety duplication system of the LV collections established Gaps in the four LV crop collection groups identified P0,P2,P3 P0,P2,P3 P0,P2,P3 Milestone description Status Partner responsible Start made selecting material used for the evaluation activities, developing core collections if needed for lettuce and spinach Done Pall Year 1 month 03 26 month 03 Start made requesting passport data for the spinach, chicory and minor vegetable databases and lacking data for the Lactuca database (ILDB) Done P0,P2,P3 month 06 Material selected for regeneration and characterization of the four crop groups Done Pall month 12 Material selected for the evaluation activities of the four crop groups Done Pall month 12 Inventory of system of safety duplication of the collections made and discussed during the second project meeting Done P0,P2,P3 Passport data of most collections of the four databases delivered Done P0,P2,P3 month 36 Databases with passport data for the four crop groups nearly finalized Done P0,P2,P3 month 36 Part characterisation and evaluation data donated and start made including these in the databases Done P0,P2,P3 month 36 Safety duplication of part of the collections realized Done P0,P2,P3 Done P0,P2,P3 Ongoing P0,P2,P3 month 42 Start made with making the databases available via the Internet month 42 Safety duplication of collections inside the project realized Ongoing P0,P2,P3 Most characterisation and evaluation data donated for inclusion in the databases Ongoing Pall Year 2 month 24 Year 3 Start made identifying gaps in the collections month 36 Year 4 month 45 Remark: this task can not be completely fulfilled at the official end of the project, as data of the fourth year will not be available for processing in the databases. The work has to be finalised after the project in the framework of the ECP/GR working group on leafy vegetables. 27 Activities 1. What has been done: Request for partners and international passport data of minor leafy vegetables is finished. 56 gene banks were contacted worldwide. Data have been only received from Italy, Romania, Greece, Poland, Sweden and USA. International passport data collected: 88 accessions of Rheum, 229 accessions of Asparagus, 3 accessions of Valerianella, 257 accessions of Eruca, 30 accessions of Diplotaxis and 40 accessions of Cynara. In total 2154 accessions of minor leafy vegetables have been collected. Harmonization of the received passport data in respect of content and form is finished. All collected passport data are available at the leafy vegetables website. Start identifying gaps in the collections of the minor crops. Analyses were started for minor leafy vegetables, and preliminary results for Asparagus were presented during the annual meeting in Cesena. Because of insufficient available information about gene pool structures and distribution areas for the majority of the minor leafy vegetables, it was decided to limit the gap analysis for minor leafy vegetables to rocket salad (Eruca and Diplotaxis). Gap analysis started for the Eruca and Diplotaxis collection. 686 Eruca accessions, 148 Erucastrum accessions and 380 Diplotaxis accessions are in the database. Analysis started with the description of the gene pool (primary, secondary, tertiary gene pool). 2. What will be done: Identifying gaps in the Eruca and Diplotaxis collections is ongoing. For this purpose suitable literature will be examined to identify to which gene pool the species belonging to and where are their distribution areas. Analysing Eruca and Diplotaxis accessions in the database recording to their origin (areas/countries) and type of material (cultivated, breeding material, landraces, wild). Comparison of the results obtained from literature and analysis of the database. Results will be shown during the next meeting in June. 3. Bottlenecks: None. Workpackage 2: Regeneration and characterization General objectives: To regenerate and characterize parts of partner’s genebank collections, for improving conservation and utilization. High priority will be given to material to be used in the evaluations, because the total numbers of accessions included in all collections needing regeneration and characterization are far too high to be handled in the framework of this project. Persons involved at IPK: Stefanie Thumm Milestones & Deliverables Deliverable description Status Partner responsible Minimum descriptors for the minor leafy vegetables concluded Done P3 Year 1 D06; month 06 Year 4 28 D07; month 45 800 accessions of lettuce characterized Done D08; month 45 110 accessions of spinach characterized Ongoing P0, P1, P3, P4, P7, P10, P13 P1, P5, P7 D09; month 45 180 accessions of chicory characterized Done P2, P8, P13 190 accessions of minor crops characterized Ongoing P0, P3, P5, P9 Characterization data entered in a fixed format to be sent to the database managers Ongoing P3 Ongoing P0, P1, P2, P3, P5, P6, P7, P10, P13 Done P0, P1, P5, P7 Ongoing P1, P2, P7, P8, P13 Done P0, P1, P2, P3, P5, P7, P9 D10; month 45 D11; month 45 D12; month 48 930 accessions of lettuce regenerated D13; month 48 50 accessions of spinach regenerated D14; month 48 200 accessions of chicory regenerated D15; month 48 70 accessions of minor crops regenerated Milestone date Milestone description Status Partner responsible Start made developing minimum descriptors for the minor leafy vegetables Done P3 month 02 month 06 Material for regeneration and characterization of the four crops selected (see also WP1) Done P3 Regeneration of material of some crops started pending the growing season Done P0, P1, P2, P3, P5, P6, P7, P8, P9, P10, P13 Done P0, P1, P2, P3, P5, P6, P7, P8, P9, P10, P13 Done P0, P1, P2, P3, P4, P5, P6, P7, P8, P9, P10, P13 Year 1 month 06 Regeneration and characterisation for all crops started month 12 Year 2 month 24 Year 3 Regeneration, characterisation of germplasm of the four collections will be continued 29 Done month 36 500 accessions of lettuce characterized month 36 70 accessions of spinach characterized month 36 110 accessions of chicory characterized month 36 110 accessions of Minor crops characterized month 36 600 accessions of lettuce regenerated month 36 30 accessions of spinach regenerated month 36 120 accessions of chicory regenerated month 36 40 accessions of minor crops regenerated Year 4 See deliverables Done P0, P1, P3, P4, P7, P10, P13 P1, P5, P7 Done P2, P8, P13 Done P0, P3, P5, P9 Done P0, P1, P2, P3, P5, P6, P7, P10, P13 Done P0, P1, P5, P7 Done P1, P2, P7, P8, P13 Done P0, P1, P2, P3, P5, P7, P9 Activities 1. What has been done: LACTUCA: 156 accessions sown on 9th of March 2010, 23 accessions sown again on 25th of March 2010 due to less germination rate (greenhouse, seeds sown into pots, approximately two weeks later transferred into trays, still in the greenhouse on tables). Before planting out in the field on 20 th of April 2010 they were kept for one week in trays outside under a roof. All 156 accessions meant for characterization, 66 of those for characterization and regeneration. 2009 harvest is completed. ERUCA: 8 accession sown on 12th of April 2010 for characterization and regeneration and 28 accessions sown on 14th of April 2010 for characterization. 2009 harvest is completed. VALERIANELLA: Harvest of 3 accessions from 2009 is finished. 16 accessions sown in August 2009 characterization is finished, harvest of 8 accessions is not finished yet. 4 accessions sown on 9th of April 2010 for characterization. 2. What will be done: LACTUCA, ERUCA: for this year’s material characterization is ongoing (this includes taking herbarium samples, pictures, morphological description). The Lactuca for regeneration in the field will still get a plastic tunnel roof before the first plants start to flower. Eruca will get several insecticide treatments against Meligethes aeneus. VALERIANELLA: Harvest of accession from 2009 will start, after seeds have dried to certain level seed cleaning will start, germination tests will be conducted. Accessions from 2010 will be characterized in summer. 30 3. Bottlenecks: Workpackage 5: Co-ordination General objectives: The general objective of this work package is the effective management of the project. Management of the project will take place on three levels: on the integral project level, on the WP level and on the crop task level. Persons involved at IPK: Andreas Börner, Ulrike Lohwasser, Stefanie Thumm Milestones & Deliverables Deliverable date WP5 Deliverable description Status Partner responsible Done P0/Pall Year 1 D27, month 01 Start-up project meeting D28, month 03 Leafy Veg website is launched on internet and active Done P0 D29, month 06 First WP coordinators meeting Done P2/P0 D30, month 11 First annual project meeting Done P1/P0 First annual progress report Second WP coordinators meeting Second annual project meeting Done Done Done P0/Pall P7/P0 P5/P0 Second annual progress report Third WP coordinators meeting Third annual project meeting Done Done Done P0/Pall P9/P0 P3/P0 Third annual progress report Fourth WP coordinators meeting Final annual project meeting Final annual progress report and workshop Done P0/Pall P8/P0 P4/P0 P0/Pall Milestone description Status Partner responsible Done P0/Pall month 01 Detailed workplan of the project defined at the start -up meeting of the project (time schedules for regeneration, characterisation and evaluation will be established) month 01 The project website will be developed Done P0 Year 2 D31, month 14 D32, month 18 D33, month 23 Year 3 D34, month 26 D35, month 30 D36, month 35 Year 4 D37, month 38 D38, month 42 D 39, month 46 D40, month 48 Milestone date Year 1 31 month 5 First WP coordinators meeting organised Done P2/P0 Done P1/P0 month 10 The second project meeting (first annual project meeting) will be organised to discuss the progress and discuss the first annual progress report First annual progress report will be finalised and submitted to the European Commission Second WP coordinators meeting organised Done P0/Pall Done P7/P0 Third project meeting (second annual project meeting) will be organised to discuss the progress and discuss the second annual progress report Done P2/P0 month 25 Second annual progress report will be submitted to the European Commission Done P0/Pall month 29 Third WP coordinators meeting organised Done P9/P0 Done P3/P0 month 34 Fourth project meeting (third annual project meeting) will be organised to discuss the progress; this meeting will also review the overall progress of the project (midterm review) in order to assess whether the following set of milestones have been fulfilled by the project Done Pall month 37 Third annual progress report including a mid-term review of the coordinator will be submitted to the European Commission month 41 Fourth WP coordinators meeting organised P8/P0 P4/P0 month 45 Final project meeting to discuss the progress report of the fourth year and the final overall project report of the project organised. A workshop presenting the results of the project will be connected to this meeting to disseminate information of the project to interesting stake holders. Fourth annual progress report and the final overall report will be submitted to the European Commission P0/ Pall Year 2 month 13 month 17 month 22 Year 3 Year 4 month 48 Activities 1. What has been done: New posters designed for the demonstration plots explaining and introducing the EU project. Most accessions of Lactuca and Eruca for characterisation are grown at IPK on demonstration plots open to visitors and employees. 32 2. What will be done: Demonstration plots will be shown to visitors in the course of the State Horticultural Show (Landesgartenschau) in Aschersleben in June. Preparation for WP coordinator meeting, preparation for the open day of the IPK (12th of June 2010) 3. Bottlenecks: None 33 P4: Palacký University in Olomouc (Palacky), Czech Republic The group at Palacký University is engaged in two workpackages (WPs), WP2: Regeneration & characterization and WP3: Evaluation. Some activities are connected with tasks in the WP1: Documentation and partner P4 develops activities leading to fulfillment of tasks in the WP5: Coordination WP 3: Evaluation Persons involved from Palacky: Aleš Lebeda, Irena Petrželová, Ivana Doležalová, Eva Křístková Deliverables (modified for partner P4), date Deliverables description Status Palacky Partner responsible in progress P4 in progress P4 Year 4 D17; month 45 D21; month 45 30 accessions of lettuce evaluated for 20 races of Bremia lactucae Evaluation data for response of 30 accessions of lettuce to 20 races of Bremia lactucae entered in a fixed format to be sent to the database managers D. Research 1. What has been done: Evaluation of response of 30 Lactuca sativa accessions to 15 races (isolates) of Bremia lactucae. 2. What will be done: Evaluation of 30 lettuce accessions for response to 5 races (isolates) of Bremia lactucae We are expecting to send evaluation data by the end of June 2010. 3. Bottlenecks: None. WP5: Co-ordination and dissemination Persons involved at Palacky: Aleš Lebeda, Eva Křístková (participation at meetings, elaboration of projects reports) 1. What has been done: Elaboration and sending project year reports (technical, finnacial) for 2009 to co-ordinator Elaboration of results obtained within the project GENRES LeafyVeg for IHC 2010 (Lisboa, Portugal): Lebeda A., Křístková E., Doležalová I., Petrželová I., Melichaříková Z., 2009. Germplasm collections of old lettuce cultivars and landraces for modern breeding. International Horticultural Congress, 22.27.8.2010, Lisboa, Portugal. 2. What will be done: what will be asked for 3. Bottlenecks: None. 34 P5: Nordic Genetic Resource Center (NordGen) The group at NGB is engaged in three workpackages (WPs), WP1: Documentation, WP2: Regeneration and Characterization, WP3: Evaluation NordGen have had the final analysis carried out and we have put the information into our databases - this include both characterisation data and evaluation data from 2009. The data is submitted (or will be right now) to the EU leafy veg project. We have also started regeneration on a few more accessions for seeds to be harvested 2010. We have included some more accessions of Lactuca serriola in our collection, but this has not been included in the project. Analysis of passport data on minor leafy vegetable crops is started and the information will be given to the project. 35 P6: Institut National de Recherche Agronomique (INRA), Montfavet, France The group at INRA is engaged in the workpackage 2; WP2: Regeneration and Characterization for regeneration of a part of the INRA lettuce collection Workpackage 2: Regeneration and characterization General objectives: To regenerate and characterize parts of the lettuce genebank collection, for improving conservation and utilization. Persons involved at INRA: Brigitte Maisonneuve and Loic Jean (+ Christophe Giraud) Milestones & Deliverables Milestone date Milestone description Status INRA Partner responsible Year 1 month 12 Year 2 Regeneration and characterisation for lettuce started Done P3 month 24 Regeneration and characterisation for lettuce will be continued Done P3 Regeneration and characterisation for lettuce will be continued Done P3 In progress P3 Year 3 month 36 Year 4 month 36 Regeneration and characterisation for lettuce will be finished Activities 4. What has been done: a. Sowing (26 November) and transplantation in peat (1 December) of the 25 winter accessions regenerated in 2009 to control the purity of the harvested seeds. Control of the type and the homogeneity on 42 young plants per progeny (30 December). b. Preparation of the third annual report for INRA: timesheets for 3 workers, financial report c. Control of the type and the homogeneity for the 25 winter accessions (plastic tunnel planted (5 January) with 32 plants per progeny; photos and description of the accessions (March) d. Sowing (22 February) and transplantation in pot (3 March) of the 70 accessions for regeneration in 2010. Control of the type and the homogeneity on the young plants. e. Plantation of the 2 plants per genotype under an insect-proof plastic tunnel (31 March) for regeneration f. Notation of some descriptors under plastic tunnel before opening of the head to improve the bolting (in April). Weekly in April-May, take care of the plants (opening of the head, removing the outer leaves) g. Sowing (15 March) and transplantation in peat (19 March) of the 49 accessions of open field varieties regenerated in 2009 to control the purity of the harvested seeds. Control of the type and the homogeneity on 28 young plants per progeny (3 April) h. Plantation of the 20 plants per progeny in a field (13 April), installation of a voile for wind protection 36 i. j. Send the safety duplication to Warwick (77 accessions regenerated in 2009, 10 May) Control in a field of the type and the homogeneity for the 49 field accessions regenerated in 2009; photos and description of the accessions (May) 5. What will be done: a. Control the passport data send in 2007 to CGN for the 300 lettuce; inform R van Treuren if there are some corrections. b. Regeneration of the 70 accessions to obtain the 300 previous accessions. Send their safety duplication to Warwick. 6. Bottlenecks: None 37 P8: Henry Doubleday Research Association (HDRA), UK HDRA is engaged in three workpackages (WPs), WP2: Regeneration and Characterization, WP3: Evaluation and WP4 Utilization & Marketing. Persons involved at HDRA: Phil Sumption Workpackage 4: Marketing Deliverable date Deliverable description Status HDRA Partner responsible 15 accessions of Minor crops evaluated for utilisation and marketing Evaluation data entered in a fixed format to be sent to the database managers complete P8 WP4 Year 4 D25; month 45 D26; month 45 Sent to WP leader Activities Evaluation of minor crops completed. 10 valerianella accessions and 10 eruca accessions evaluated. Evaluation data sent entered in a fixed format and sent to the WP leader. Workpackage 5: Coordination Activities Talk given to Mercia Organic Producers. January 2010 ‘Rocket Science and other leafy vegetables’ by Phil Sumption. Article in The Organic Grower – January 2010. Lamb’s lettuce/corn salad pp36-37 38 P10: University of Bologna, Department of Agroenvironmental Science and Technology (DiSTA-UNIBO), Bologna, Italy WP 1: Documentation Persons involved at UNIBO: L. Filippo D'Antuono, Manuela Agata manco Deliverable date Deliverable description Status DiSTA-UNIBO Partner responsible in course for additional chicory accessions Status DiSTA-UNIBO P0,P2,P3 Done Pall Done Pall P0,P2,P3 Passport data of most collections of the four databases delivered in course for additional chicory accessions P0,P2,P3 Databases with passport data for the four crop groups nearly finalized in course rro additional chicory accessions WP1 Year 3 D01; month 30 Passport data of the four leafy vegetable (LV) collections donated Milestone date Milestone description Partner responsible Year 1 month 03 month 12 Start made selecting material used for the evaluation activities, developing core collections if needed for lettuce and spinach Material selected for the evaluation activities of the four crop groups Year 2 month 24 Year 3 month 36 39 month 36 Part characterisation and evaluation data donated and start made including these in the databases done for analytical data of rocket salad and sensory data of rocket salad and chicory P0,P2,P3 The passport data of additional 24 chicory accessions (not yet in Gene banks), that have been received by Sativa seeds and services, and have been evaluated analytically and sensorially, were supplied to P2. Seeds of these same accessions are being collected and will be supplied to P2 as well. Workpackage 3: Evaluation General objectives: Persons involved at UNIBO: L. Filippo D'Antuono, Manuela Agata Manco, Federico Ferioli Milestones & Deliverables Deliverable date Deliverable description WP3 Status DiSTAUNIBO Partner responsible Year 1 D16; month 12 Seeds of the selected material of the four crop groups sent to the partners involved seeds of Eruca received from P3 Year 4 D19; month 45 265 accessions of chicory evaluated evaluation started; analytical activities in course done for rocket salad (37 accessions) D20; month 45 49 accessions of minor crops evaluated Milestone date Milestone description Status DiSTAUNIBO Material selected for the evaluation activities of the four crop groups (see WP1) Done Year 1 month 12 Partner responsible 40 Year 2 done month 18 Evaluations started Year 3 month 36 month 36 170 accessions of chicory evaluated 30 accessions of minor crops evaluated evaluation started; analytical activities in course done for rocket salad (37 accessions) Activities Eruca and Diplotaxis evaluation for glucosinolates The final analytical evaluation of rocket salad glucosinolates have been finished during the period. The data were send to WP leader according to the standard format Chicory evaluation for sesquiterpene lactones A total of 102 accession were considered; 24 were received from Sativa seeds and services and the remaining from Geves. 70 accessions belonged to different types of C. inthybus and 32 to C. endivia. Seeds were sown in trays on July 25 2010 and transplanted in the field on August 17. harvest started on October 27 and went on until Christmas, according to precocity. Five late Treviso types were seriously damaged by an early frost, and were not harvested. The harvested material was trimmed and the edible part separated from waste. On both parts, water content was determined, and both were freeze-dried. 8 additional eight witloof accessions (the edible product were received from P2 in March 2010, and were prepared in the same way as the others. Total number of accession submitted to analysis was therefore 105. Until now the activities done were the following: - Preliminary purification trials by means of TLc and direct and reverse phase SPE. Chosen direct phase SPE. - Preliminary extraction trials with different solvent mixtures: chosen methanol:water, 4:1 + 2% formic acid. - sample lyophilisation - sample extraction - purification of free lactones - recovery of bound lactones by cellulase treatment - purification of total lactones - start HPLC injections 41 Workpackage 4: Marketing Persons involved at DiSTA-UNIBO: L. Filippo D'Antuono, Manulea Agata Manco Deliverable date Deliverable description WP4 Status DiSTAUNIBO Partner responsible Year 1 Done D22; month 12 Seeds of the selected material of the three crop groups sent to the partners involved Year 4 D24; month 45 125 accessions of Chicory evaluated for utilisation and marketing D25; month 45 15 accessions of Minor crops evaluated for utilisation and marketing Milestone date Milestone description sensory evaluation of 28 endives done P8/P12 sensory evaluation of 37 rocket salads done Status P9/P10 Done P2/P3/P7/P8/P9 / P10/P12/P13 + companies Done P7/P8/P9/ P10/P12/P13 sensory evaluation of 28 endives done sensory evaluation of 37 rocket salads done P8/P12 Partner responsible Year 1 month 12 Material selected for utilization and marketing Year 2 month 18 Activities on utilisation and marketing started Year 3 month 36 month 36 75 accessions of chicory evaluated for utilisation and marketing 10 accessions of minor crops evaluated for utilisation and marketing P9/P10 During the autumn 2010, 28 endive accessions have been evaluated by means of sensory analysis. Preliminary a was selected and trained for the evaluation of the three specific characters: bitter, herbaceous, astringent, that were judged as significant for the evaluation of the available material. 42 The evaluation sessions were carried out according to the availability of material, but never including more than 6 samples per days, to avoid tiring and carry over effects Workpackage 5: Coordination Persons involved at DiSTA-UNIBO: L. Filippo D'Antuono, Manuela Agata Manco, Federico Ferioli Milestones & Deliverables Deliverable date WP5 Deliverable description Status DiSTAUNIBO Partner responsible attended P0/Pall attendance was not possible for family reasons P1/P0 attended P2/P0 Year 1 D27, month 01 D30, month 11 Start-up project meeting First annual project meeting Year 2 D33, month 23 Second annual project meeting Year 3 D34, month 26 D35, month 30 Second annual progress report Third WP coordinators meeting D36, month 35 Third annual project meeting organised and hosted in Cesena P0/Pall P9/P0 P3/P0 Year 4 planned to be attended P4/P0 Milestone description Status DiSTAUNIBO Partner responsible Detailed workplan of the project defined at the start -up meeting of the project (time schedules for regeneration, characterisation and evaluation will be established) The second project meeting (first annual project meeting) will be organised to discuss the progress and discuss the first annual progress report done / attended P0/Pall not attended for family reasons P1/P0 D 39, month 46 Final annual project meeting Milestone date Year 1 month 01 month 10 43 Year 2 month 22 Third project meeting (second annual project meeting) will be organised to discuss the progress and discuss the second annual progress report attended P2/P0 Fourth project meeting (third annual project meeting) will be organised to discuss the progress; this meeting will also review the overall progress of the project (midterm review) in order to assess whether the following set of milestones have been fulfilled by the project organised and hosted in Cesena P3/P0 planned to attend P4/P0 Year 3 month 34 Year 4 month 45 Final project meeting to discuss the progress report of the fourth year and the final overall project report of the project organised. Activities Third year scientific and financial reports were regularly delivered, including some further clarifications. The UNIBO team organised and hosted the fourth Consortium meeting in Cesena, Italy, on 17-18 November 2009 44 P10: Agricultural Institute of Slovenia, KIS, Ljubljana, Slovenia No information yet received 45 P11: ProSpecieRara, Aarau, Switserland Workpackage 3: Evaluation General objectives: evaluation of marketability of leaf chicory and endive Milestones & Deliverables Deliverable date WP3 Deliverable description Status Partner responsible Ready to deliver P8 Evaluation of marketability of selected varieties Milestone description Status Partner responsible Planning of degustation; varieties still growing P8 Year 4 Dec 2009 – May 2010 Milestone date Year 4 June 2010 – September 2010 Selected varietes of endive and leaf chicory will be tested for their sensory value Workpackage 2: Characterization General objectives: characterization of selected leaf chicory and endive varieties Milestones & Deliverables Deliverable date WP2 Deliverable description Status Partner responsible P8 Milestone description Time of bolting and flower coloration missing, wil be evaluated this summer 2010 Status - - - Year 4 Dec 2009 – May 2010 Milestone date Year 4 Characterisation of 6 endive and 45 leaf chicory varieties Partner responsible 46 Activities 1. 2. Missing characterisation/evaluation/marketability data for 2007/2008 (11 leaf chicory, 12 endive; 8 leaf chicory, 28 endivia) demanded from the partners. Composition of the data is ongoing. Due to late planting time 2009 (6 endive, 45 leaf chicory) from GEVES,CHERAC, PSR, Samen Mauser the varieties bolted only in 2nd year. These Data is still missing. Additionally some of the varieties did not get through hibernation and had to be resawn (6 endive, 13 leaf chicory). There will be organized a degustation event of the resawn varieties. The missing data of bolting time and flower coloration will be evaluated.