Supporting Information - Springer Static Content Server

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Supporting Information - Springer Static Content Server
Supporting Information
Increasing Protein Charge State when using Laser Electrospray Mass Spectrometry
Santosh Karki, Paul M. Flanigan IV, Johnny J. Perez, Jieutonne J. Archer,
Robert J. Levis
Department of Chemistry and Center for Advanced Photonics Research, Temple University,
Philadelphia, PA 19122
Figure S1. The mass spectra resulting from laser induced vaporization of 1mM aqueous
cytochrome c into the electrospray solvent (ES) of a) 5mM ammonium acetate and b)
0.4% m-NBA in 5mM ammonium acetate, c) the laser vaporization of 1mM cytochrome
c with 5% acetic acid into the ES of 5mM ammonium acetate, and the ESI mass spectra
of 10μM cytochrome c in d) 5mM ammonium acetate, e) 0.4% m-NBA and 5mM
ammonium acetate, and f) 5% acetic acid and 5mM ammonium acetate solution. Note:
Ammonium acetate was prepared in 1:1 (v:v) water: methanol.
Additional information on protein’s (cytochrome c and lysozyme) conformation under
different pH conditions using LEMS has been previously published (ref 43).
Figure S2. The mass spectra resulting from laser induced vaporization of 1mM aqueous
myoglobin into the electrospray solvent (ES) of a) 5mM ammonium acetate and b) 0.4%
m-NBA in 5mM ammonium acetate, the c) laser vaporization of 1mM myoglobin with
1% acetic acid into the ES of 5mM ammonium acetate, and the ESI mass spectra of
10μM myoglobin in d) 5mM ammonium acetate, e) 0.4% m-NBA and 5mM ammonium
acetate, and f) 5% acetic acid and 5mM ammonium acetate solution. Note: Ammonium
acetate was prepared in 1:1 (v:v) water: methanol.
Figure S3. The mass spectra resulting from laser induced vaporization of 1mM aqueous
lysozyme into the electrospray solvent (ES) of a) 5mM ammonium acetate and b) 0.4%
m-NBA in 5mM ammonium acetate, and the ESI mass spectra of 10uM lysozyme
prepared in c) 5mM ammonium acetate and d) 0.4% m-NBA in 5mM ammonium acetate.
Note: Ammonium acetate was prepared in 1:1 (v:v) water: methanol.
Figure S4. Mass spectra representing laser induced vaporization of a) disulfide intact and b) disulfide
reduced lysozyme into the ES solvent consisting of 1:1 (v:v) water:methanol with 0.4% m-NBA and 0.1%
formic acid. The pH of the solution was measured to be 2.5.

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