이력서 (최종현)

Comments

Transcription

이력서 (최종현)
High-Throughput Screening System and
Its Biotechnological Applications
Jong Hyun Choi
Applied Microbiology Research Center, Bio-Materials Research Institute,
KRIBB, 1404 Sinjeong-dong, Jeongeup, Jeonbuk 580-185, Korea
Novel enzyme and/or metabolic pathway screening techniques from various environmental
resources and its biotechnological applications have widely expanded in the past few years. However,
it is difficult to screen new enzymes due to limited assay protocol, expensive substrate, library, low
sensitivity, low activity of target enzymes in host strain, and time-consuming and laborious process.
Therefore it was difficult for mass screening of target enzymes from various resources.
Our research team has developed the HTS system for enzyme screening from various environmental
resources including metagenome. This HTS system composed with Robotic Integration System,
JANUS Liquid Handling System, VICTOR3 Multilabel Microplate Reader, K3 Colony Picking
System, Automated Incubator, Carousel, Lidding/Delidding System, Bar Code Reader, FACSAria
High-speed Flow Cytometer. Finally optimized screening methods for high throughput system led to
over 105 clone active verification per day were available (Figure 1).
Figure 1. HTS system for screening of enzyme/metabolic pathway in KRIBB
In this study, we developed mass screening methods for various enzymes from metagenomic
libraries using HTS system based on robot. We could new enzymes such as cellobiohydrolases,
glycosyltransferases, BVMO, cold-adapted esterase and deoxyribose 5-phosphate aldolase(DERA)
based on fluorescence intensity. These new strategies combined with HTS system could screen various
new enzyems/metabolic pathways more fast, sensitive and easy than previously reported screening
methods. This approach would be applied for other useful enzyme and metabolic pathway screening
from metagenomic resources.
Biographical Statement
Jong Hyun Choi
Senior Researcher
Enzyme-Based Fusion Technology Research Team
Applied Microbiology Research Center
Bio-Materials Research Institute
Korea Research Institute of Bioscience and Biotechnology(KRIBB).
Office Address:
Enzyme-Based Fusion Technology Research Team
Applied Microbiology Research Center
Bio-Materials Research Institute
Korea Research Institute of Bioscience and Biotechnology(KRIBB)
181 Insin-gil, Jeongeup-Si, Jeonbuk, 580-185, Republic of KOREA
(Tel: +82-63-570-5150. +82-10-4719-7562, Fax: +82-63-570-5109, e-mail: [email protected])
Home Address:
Ganaparosvill-2 605, 894-2 Jijok-dong, Yuseong-gu, Daejeon 305-330, Korea
(Tel: 042-477-1108, 010-4719-7562)
Position:
2008. 10.
2008. 04.
2007. 09.
2005. 09.
2003. 02.
2003. 02.
–
–
–
–
present.
Senior Researcher, Korea Research Institute of Bioscience and Biotechnology.
2008. 09. Research Scientist, Korea Research Institute of Bioscience and Biotechnology.
2008. 03. Research Professor, BioProcess Engineering Research Center, KAIST
2007. 08. Research Professor, Dept. Chem. and Biomolecular Eng., KAIST
– 2007. 08. Senior Researcher, BioProcess Engineering Research Center, KAIST
– 2008. 03. Senior Researcher, Bioinformatics Research Center, KAIST
Educational Background
1991. 3. – 1994. 8.
1995. 3. – 1997. 2.
1997. 3. –
Dept. of Biological Engineering, Inha Univ., Incheon, Korea (B.S.)
Dept. of Biological Sciences, KAIST, Daejeon, Korea (M.S.)
(Advisory Professor : Gyun Min Lee)
2003. 2. Dept. of Chemical and Biomolecular Engineering, KAIST, Daejeon, Korea
(Ph.D.) (Advisory Professor : Sang Yup Lee)
Experience and Academic/Research Activity:
1. Member, Korea Institute of Biotechnology and Bioengineering (2004 – present) (평위원)
2. Member, The Microbiological Society of Korea (2004 - present)
3. Member, Korean Society for Microbiology and Biotechnology (2004 - present) (평위원)
4. Member, Korean Institute of Chemical Engineers (2004 - present)
5. Reviewer, Biotechnology and Bioengineering (2004 – present)
6. Reviewer, Bioprocess and Biosystems Engineering (2004 – present)
7. Reviewer, Journal of Microbiology (2005 – present)
8. Reviewer, Biotechnology Journal (2006 – present)
9. Reviewer, Journal of Microbiology and Biotechnology (2005 – present)
10. Local Organizing Committee Member, International E. coli Association Symposium on Systems Biology
2006 (IECA2006), Jeju Island (2005 - 2006)
11. Poster Session Chair, International E. coli Association Symposium on Systems Biology 2006 (IECA2006),
Jeju Island (2005 - 2006)
12. Local Organizing Committee Member, Metabolic Engineering VIII (2009 - 2010 )
13. Reviewer, BMC systems biology (2011-prsent)
14. Reviewer, FEMS Microbiology Letters (2013-prsent)
Teaching:
1. Bioinformatics (Undergraduates) (2004)
2. Process for Recombinant Microorganisms (Graduates) (2005)
3. Biomolecular Engineering (Undergraduates) (2006)
Dissertation Title
M. S. Thesis
Ph. D Thesis
Effect of temperature and serum concentration on retroviral vector production
from pMFG/ΨCRIP packaging cell line
Development of systems for secretion and cell surface display of proteins in
Escherichia coli
Research Interest
Systems Biotechnology
Synthetic Biotechnology
High Throughput Screening
Metabolic Engineering
Enzymology
Metagenomics
Single Cell Genomics
Protein Technology
- Directed evolution
- Cell surface display
- Secretion of recombinant proteins from microorganism
- Extracellular production of recombinant proteins
- Bioconversion
Recombinant Protein Production
High cell Density Culture
Transcriptome Analysis
Genomic Engineering
Nanobiotechnological Applications
Research Projects (연구책임자)
1. 시스템대사공학을 위한 메타게놈 자원 유래 High-throughput toolbox 개발, 2012. 5. –
2021. 4. 기후변화 대응사업, 교육과학기술부, 900,000,000원
2. 합성 생물학 기반 High Throughput 원천 기술 개발, 2011. 8. – 2020. 7. 글로벌
프론티어 사업, 교육과학기술부, 900,000,000원
2. 메타게놈 자원을 활용한 산업용 균주 개발을 위한 호스트-벡터 시스템 개발, 2009. 5. –
2012. 4. 교육과학기술부, 129,000,000원
3. 응고점이 -15C 까지 낮아진 불포화도가 증가된 팜유 유래 바이오디젤의 효소 공정
개발 및 분자진화를 통한 고활성 불포화 효소 개발, 2006. 5. - 2008. 3., 산업자원부,
80,000,000원
4. 나노 패터닝 방법을 이용한 바이러스성 질병진단용 고감도-실시간 바이오칩 개발, 2006.
3. - 2008. 2., 중견연구자지원사업, 과학기술부, 140,000,000원
5. 고지혈증 치료제 statin계 의약중간체 연료 제조기술개발, 2005. 3. - 2007. 2., 산업자원부,
80,000,000원
6. 충청권 바이오 혁신역량 강화 사업, 2004. 9.- 2007. 6., 산업자원부, 420,000,000
7. 방사선 동위원소를 이용한 카이네이즈 반응 검출 칩 개발, 2004. 6. - 2005. 5.,
과학기술부, 24,000,000원
8. 분자진화에 의한 메탄자화 효모용 MOX 프로모터 개발, 2002. 9. - 2003. 8., 산업자원부,
37.784,000원
9. 대장균에서 효율적인 L-carnitine 생산시스템 개발, 2002. 9. - 2003. 8., 산업자원부,
30,000,000원
Publications
Selected International Journals
1. Cheong, D-E., Choi, J.H., Song, J.J. and Kim, G-J. "Construction of non-invasively constitutive
expression vectors using a metagenome-derived promoter for soluble expression of proteins",
Bioprocess Biosyst. Eng. DOI 10.1007/s00449-013-0890-x, in press. (2013)
2.
Ko, K.C., Rim, S.O., Han, Y.J., Shin, B.S., Choi, J.H. and Song, J.J. "Identification and
characterization of a novel cold-adapted esterase from a metagenomic library of mountain soil". J.
Ind. Microbiol. Biot. 39:681–689 (2012.05) (Correspondence Author)
3.
Ko, K.C., Han, Y.J., Choi, J.H., Kim, G.J., Lee, S.G. and Song, J.J. "A novel bifunctional
endo/exo-type cellulase from anaerobic ruminal bacterium", Appl. Microbiol. Biotech. 89(5)
1453-1462 (2011)
4.
Yoon, J.H., Choi, J.H., Kang, S.J., Choi, N.S., Lee, J.S., and Song, J.J. "Jeongeupia
naejangsenensis gen. nov., sp. nov., a cellulose-degrading bacterium isolated from forest soil from
Naejang Mountain in Korea", IJSEM, 60:615-619 (2010)
5.
Qian, Z.G., Xia, X.X., Choi, J.H., and Lee, S.Y. "Proteome-based identification of fusion
partner for high-level extracellular production of recombinant proteins in Escherichia coli",
Biotechnol. Bioeng. 101(3), 587-601 (2008. 10.) Selected as a spotlight paper
6.
Huh, Y.S., Choi, J.H., Park, T.J., Hong, Y.K., Hong, W.H., and Lee, S.Y. "Microfluidic cell
disruption system employing a magnetically actuated diaphragm", Electrophoresis, 28: 4748-4757
(2007). (equally contributed)
7.
Choi, J.H. and Lee, S.Y., "Production of recombinant proteins by high cell density culture of
Escherichia coli", Chem. Eng. Sci., 66: 876-885. (2006).
8.
Choi, J.H. and Lee, S.Y., "Secretory and extracellular producion of recombinant proteins using
Escherichia coli", Appl. Microbiol. Biotechnol., 64 : 625-635 (2004).
9.
Choi, J.H., Lee, S.J., Lee, S.J., and Lee, S.Y., "Enhanced production of IGF-I fusion protein in
Escherichia coli by the coexpression of the down -regulated genes identified by transcriptome
profiling", Appl. Environ. Microbiol., 69(8): 4737-4742 (2003).
10. Lee, S. Y., Choi, J.H. and Xu, Z. "Microbial cell surface display", Trends Biotechnol. 21: 45-52
(2003)

Similar documents