the BlueCore Fact Sheet

 BlueCore™ Product Introduction INTRODUCTION Based upon a protected formulation of citric fruit terpenes, BlueCore™ is an all-­‐natural, 100% biodegradable, and completely non-­‐toxic liquid with broad applications. The product has been certified completely organic by IFOAM (International Federation of Organic Agriculture Movements), is Kosher Certified, non-­‐carcinogenic, non-­‐mutagenic and its ingredients are all generally recognized as safe (GRAS) by the U.S. Food & Drug Administration (FDA). BlueCore’s broad effectiveness provides impressive results in a wide range of operations and public facilities including, Hotels, Restaurants, Health Centers, Offices / Office Equipment, Food Processing Plants, Stockyards, and a host of other venues with odor, biological exposure, and pest issues. Applied as a spray, liquid additive, or hand-­‐wiped, BlueCore™ remains effective for extended periods without reapplication or residues, can be mixed with other products, is non-­‐
corrosive, non-­‐flammable, non-­‐reactive, requires no special precautions or personal protective equipment, and does not create human danger if dosage or dilution is exceeded. PRODUCT LINES The BlueCore™ backbone is used to create five (5) products. Each product is made with different formulation and intended for different applications. The BlueCore™ product line includes a sanitizer, disinfectant, deodorizer, hydrolyzer, and a pest repellant. BlueCore™ Sanitizer. On contact, the sanitizer effectively eliminates fungi, mycotoxins produced by fungi, yeasts, bacteria (gram negative and positive), spores, viruses (e.g., Dengue, Hantavirus, Influenza [AH1N1, B, & C], equine encephalitis or West Nile virus, enterovirus, etc.) and more. BlueCore™ Disinfectant. The disinfectant effectively controls the spread of disease-­‐causing organisms within a short period of time, usually within 10 minutes, after application. Its formulation is a diluted form of the sanitizer. BlueCore™ Deodorizer. The deodorizer is an extremely effective agent for the elimination of a wide array of odors, including smells emanating from ammonia, smoke, food, trash, and almost any other undesirable odor. With an impressive diffusion capacity, the deodorizer quickly eliminates odors within a standard size room or area using one or two sprays from a common manual pump spray bottle. BlueCore™ Hydrolyzer. The hydrolyzer is powerful all natural agent to prevent the buildup of grease and fats on floors, grease traps, venting systems, pipes and other surface areas. The hydrolyzer can be used to liquefy or hydrolyze old grease of fatty buildup for easy removal and continued easy maintenance. GreenHawk Global Corporation™ Main: 4500 I-­‐55N, Suite 205N | Jackson, MS 39211 Satellite: 4925 Greenville Ave., Suite 200 | Dallas, TX 75206 th
Canada: 439 University Ave., 5 Floor | Toronto, ON M5G 2N8 P: 888.761.8730 | F: 888.761.8729 | www.ghwk.com | Jackson | Dallas | Toronto BlueCore™ Pest Repellant & Control. The pest control agent kills most insect and pest larvae, eliminates food sources of pests and, thus, controls and eliminates mature pest infestations. BlueCore™ Pest Control can be used to effectively eliminate mosquito, bed bug, and other troublesome pest infestations. APPLICATIONS & COMMERCIAL USES BlueCore™ is currently deployed in a number of commercial applications demonstrating its broad usefulness. The product is very cost effective, can be delivered in bulk and large concentrated containers, is easy to dilute and maintains an extended shelf life. Cheese Manufacturing. BlueCore™ is used by a Mexican dairy and cheese producer, Quesos La Risueña, to improve its finished cheese packaging process and to extend its early product shelf life. Risueña dips in finished cheese products in a BlueCore™ solution prior to packaging, which eliminates product-­‐spoiling bacteria, ensures more stable and safe finished packaging procedure, and extends product shelf life to a desired degree. Sugar Production. BlueCore™ has been adopted by eleven (11) sugar mills in Mexico and Central America for the control of feedstock spoilage and more effective sucrose production. BlueCore™ is applied to sugar cane feedstock that has been staged for processing and has dramatically slowed or eliminated cane percentage spoilage prior to entering the milling process. Within the milling process, BlueCore™ is substituted for the common processing additives (e.g., dithiocarbamate, chlorine dioxide, or quaternary ammonium salts) and provides more effective production of sucrose, eliminates process downtime, and significantly reduces equipment maintenance activities. It’s primary action is to eliminate Leuconostoc bacteria and the resulting G6PD enzyme of the bacteria that drives unwanted process byproducts that act to reduce pure sucrose production (see attached data “El Potrero Sugar Cane Mill”). Hotel & Hospitality. BlueCore™ is currently being successfully used for a wide array of applications within the Tryp Hotel in Mexico City, a Wyndham Hotel brand facility. BlueCore™ is used to provide better and more consistent odor control, is used to sanitize bathroom areas, is applied to laundry processes for linen disinfection, is used on food surfaces and food storage areas in hotel restaurants, and is applied to room carpets and mattresses to ensure the prevention of pest infestation, particularly exposure to bed bugs. Shrimp Management. BlueCore™ is currently used to eliminate a traditionally difficult shrimp fungus in seven (7) shrimp nurseries in New Caledonia, 750 miles off the coast of Australia. The shrimp nurseries had deployed a number of methods in attempts to control the fungus problem, including aircraft flyover applications of expensive chemicals, without success. BlueCore™ is applied in the larval stages of shrimp production and has eliminated the occurrence of the fungus through to mature shrimp development and has, thus, eliminated the shrimp spoilage problem. GreenHawk Global Corporation™ Main: 4500 I-­‐55N, Suite 205N | Jackson, MS 39211 Satellite: 4925 Greenville Ave., Suite 200 | Dallas, TX 75206 th
Canada: 439 University Ave., 5 Floor | Toronto, ON M5G 2N8 P: 888.761.8730 | F: 888.761.8729 | www.ghwk.com | Jackson | Dallas | Toronto Bus Sanitization. BlueCore™ is currently used by ETN / TuriStar, and high end bus and tour company in Mexico City, for sanitization and deodorization of their vehicle fleet. BlueCore™ products have been proven less expensive, easier and more timely to apply, more effective, and do not create troublesome residues, do not cause rust or fabric harm, and provide a superior passenger experience without toxic chemical exposure. Point of Presence Hand Sanitizer. BlueCore™ has been adapted as a hand sanitizer and has been initially deployed at the ticket counters of Volaris Airlines, in Mexico City. The formulation has been well received and provides more effective sanitization than the competitors, with a more pleasant fragrance, without using glycerin carriers, and providing a more persistent and longer lasting protection against disease-­‐causing microbes. In addition to the above commercial applications, BlueCore™ can be deployed to eliminate the use of chlorine in pools, can be added to the manufacture of wallboard for the control or elimination of mold, can be used in backyard / outdoor misting systems for the non-­‐toxic control of mosquitos, may be applied to control or eliminate agricultural diseases such as the Panama Disease (TR4) fungus causing banana damage worldwide, and a host of other productive uses. SCIENTIFIC SUPPORT INIFAP, the Mexican National Institute of Forestry, Agriculture and Livestock Research, conducted laboratory testing of BlueCore™ and its effectiveness against fungi and bacteria, in 2010. The study (copy attached, with English translation) used strains of fungi and bacteria representative of microbial subcategories that are generally recognized as the more difficult and prevalent microbes occurring in everyday human activity. The study demonstrates the sanitizing and disinfecting efficacy of BlueCore™ in varying concentrations. PRODUCTION CAPACITY & PRICING Currently, BlueCore™ can be manufactured to accommodate orders of 30 to 40 tons of product per month. With greater distribution and volume requirements, BlueCore™ production capacity can be scaled to accommodate monthly production needs many orders of magnitude greater. Depending upon the application and required dilutions, GreenHawk can profitably distribute BlueCore™ products at pricing levels representing less than a penny per ounce. As expected, bulk orders reduce pricing. RELATIONSHIP TO THE PRODUCT Currently, GreenHawk is a U.S. sub-­‐licensee of the exclusive license holder of BlueCore™, QFC North America, LLC. World Water Group, Inc. works in joint venture with GreenHawk for the commercialization of GreenHawk’s entire suite of environmental technologies. World Water Group is a minority shareholder of GreenHawk. BlueCore™ is a GreenHawk owned brand for the product in the United States and Canada. GreenHawk Global Corporation™ Main: 4500 I-­‐55N, Suite 205N | Jackson, MS 39211 Satellite: 4925 Greenville Ave., Suite 200 | Dallas, TX 75206 th
Canada: 439 University Ave., 5 Floor | Toronto, ON M5G 2N8 P: 888.761.8730 | F: 888.761.8729 | www.ghwk.com | Jackson | Dallas | Toronto EL POTRERO Sugar Cane Mill (Mexico)
The Sugarcane mill El Potrero in Mexico processes about 450 Metric Tons of Sugar Cane per hour.
During the 4 months of the harvest season, the mill operates 24/7.
TABLE 1
Average Results (March 31, 2011 to April 5, 2011) for the Amber color Bactericide BEFORE the BlueCore test BX
22.30
RAW JUICE (PRIMARY)
POL
PUR
RDS
19.13 85.78 0.843
pH
5.2
BX
18.83
MILLED JUICE (Water added)
POL
PUR
RDS
15.71
83.43
0.799
pH
5.2
BX
17.69
CLARIFIED JUICE
POL
PUR
RDS
15.38
86.95
0.767
pH
7.0
Average Results for the Inhibitor BLUECORE during the test (April 6-­‐10, 2011)
BX
22.03
RAW JUICE (PRIMARY)
POL
PUR
RDS
pH
18.87 85.69 0.989
5.1
warmer weather
BX
18.84
MILLED JUICE (Water added)
POL
PUR
RDS
15.65
83.03
0.898
pH
5.1
BX
17.77
CLARIFIED JUICE
POL
PUR
RDS
15.34
86.35
0.778
pH
7.1
LOSS FORMULA: Kgs / MTSC = 0.099 x (Brix1) x (C2-­‐C1) => Kgs / TCM = 0.099 x (Brix1) x ({RDS2 x 100 / Brix2} -­‐ {RDS1 x 100 / Brix1})
Whereas:
0.099 = Is a constant (correction factor) . It is the relationship using the Fehling Solution (sucrose and copper) Brix1 = Brix average taken from samples of the primary, raw juice
C1 = Grams of reducing sugars for each 100 gr of dried solids sampled from the primary, raw juice => C1 = RDS1 x 100 / Bx1
C2 = Grams of reducing sugars for each 100 gr of dried solids sampled from the milled juice => C2 = RDS2 x 100 / Bx2
MTSC = Metric Ton of Milled Sugar Cane
C1 = 3.7803
C2 = 4.2432
C2 -­‐ C1 = 0.4630
C1 = 4.4893
C2 = 4.7665
C2 -­‐ C1 = 0.2771
Kgs / MTSC = 1.0221 kgs of sucrose LOST per Metric Ton of Sugar Cane milled per day in the Milled Juice using the Amber Coler Bactericide
Kgs / MTSC = 0.6044 kgs of sucrose LOST per Metric Ton of Sugar Cane milled per day in the Milled Juice using our BlueCore Inhibitor
Difference:
0.4177 kgs of sucrose NOT LOST per Metric Ton of Subar Cane milled per day in the Milled Juice by replacing the color amber bactericide with our Inhibitor BlueCore
Since EL POTRERO Sugar Cane Mill -­‐ processes 450 MT of sugar cane per hour,
-­‐ processes 24 hours a day during the harvest season, and
-­‐ the harvest season lasts 120 days, then
The total harvest season SAVINGS obtained by replacing the Amber color Bactericide with BlueCore Inhibitor are:
1.0221 Kg/MT x 450 MT/hr x 24 hr/d x 120 d 1,324,611
=
Kg, or
1325 Metric Tons of Sucrose
0.6044 Kg/MT x 450 MT/hr x 24 hr/d X 120 d = 783,295
Kg, or
783
Metric Tons of Sucrose
0.4177 Kg/MT x 450 MT/hr x 24 hr/d x 120 d 541,316
= Kg, or
541
Metric Tons of Sucrose
brix -­‐ amount of soluble solids in juice
pol -­‐ amount of sucrose within the solid of the brix
pur -­‐ purity is (pol / brix)*100
rds -­‐ reduction sugars (lower is better)
1nst1tuto Nacional de Investigaciones
Forestales, Agricolas y Pecuarias
"2010, Aiio de Ia Patria. Bicentcn;u·io del lnicio de Ia
lndepcndencia y Centcnario dellnino de Ia Rcvoluci6n".
29 de Noviembre de 2010
[Manufacturer / Inventor
Redacted for Protection]
Present e:
Por este conducto le informo los resultados obtenidos al realizar bioensayos con el producto
BlueCore Bio-3107 con relaci6n a su respuesta sobre los hongos Phytophthora capsici,
Rhizoctonia so/ani. Fusarium sp
y Trichoderma sp.
y las bacterias: Erwinia sp.,
Pseudomonas sp. y Xanthomonas sp.
Tipo de material sometido a bioensayo: BlueCore Bio-3107
Tipo de bioensayo:
Evaluaci6n del producto BlueCore Bio-3107 en medio de cultivo Papa-Dextrosa-Agar para
con relaci6n a su respuesta a los hongos Phytophthora capsici, Rhizoctonia so/ani, Fusarium
sp y Trichoderma sp. y las bacterias: Erwinia sp.. Pseudomonas sp. y Xanthomonas sp.
Metodologia:
Se incorpor6 el producto BlueCore Bio-3107 en el medic de cultivo PDA (papa- dextrosaagar). Los tratamientos registrados en el Cuadro 1, se aplicaron al memento del vaciar el
medio de cultivo en cajas de Petri a una temperatura de 30 a 40 °C. Una vez aplicadas las
dosis a los medics de cultivo se dejaron enfriar para su solidificaci6n. Cuando el medio de
cultivo solidific6, se rotularon y se procedi6 a sembrar de manera individual a los hongos
Phytophthora capsici, Rhizoctonia so/ani, Fusarium sp y Trichoderma sp. y las bacterias:
Erwinia sp., Pseudomonas sp. y Xanthomonas sp. Una vez sembrados se sometieron a
incubaci6n a 25 oc durante 15 dias (Los bioensayos se iniciaron el 26 de octubre de 2010).
Se realizaron observaciones diarias y para venficar el comportamiento de los
microorganismos involucrados al estar en contacto con el producto BlueCore Bio-3107 el
'
dia 9 de noviembre de 2010 se tomaron fotografias que se anexan. Se realizaron
observaciones hasta 29 de noviembre de 2010.
25 Aniversario Ciencia y Tecnologia para elCampo Mexicano
Campo Experimental Bajio
Km. 6.5 Carretera Celaya-SanMiguel de Allende. Mpio. Celaya. Guanajuato
C.P. 38110. AP.112. Tel. (461) 611 5323. Fax: (461) 611 5431
www.inifap.gob.mx
lnstituto Nacionalde Investigaciones
Forestales. Agricolas y Pecuarias
"2010, Ano de Ia Patna. Bicentcnario del I nino de Ia
Indepcndenc1a y Centcnano del !nino de Ia Rcvoluci6n"
Cuadro 1. Tratamientos del producto BlueCore Bio-3107 en bioensayos en medio de
cultivo PDA en cajas Petri con respecto a su respuesta
contra los hongos
Phytophthora capsici, Rhizoctonia so/ani, Fusarium sp y Trichoderma sp. y las
bacterias: Erwinia sp., Pseudomonas sp. y Xanthomonas sp. Celaya, Gto. 2010
Tratamientos
Dosis/Caja
--------------------------------
1. BlueCore Bio-3107 200 ml de producto/10 L de agua
2. BlueCore Bio-3107 400 ml de producto/10 L de agua
3. BlueCore Bio-3107
752 ml de producto/10 L de agua
4. BlueCore Bio-3107 1000 ml de producto/10 L de agua
(Dosis Comercial)
5. BlueCore Bio-31071200 ml de producto I 10 L de agua
0.5ml
1 ml
1.88 ml
2.5 ml
3ml
6.Testigo (Medic de cultivo sin producto)
Resultados
En funci6n de las observaciones realizadas diariamente durante el desarrollo de los
bioensayos, se obtuvieron los siguientes resultados:
En el caso de los hongos Phytophthora capsici Rhizoctonia so/ani, Fusarium sp y
sp. sometidos a los tratamientos del producto BlueCore
Bio-3107
especificados en el Cuadro 1. Se present6 una inhibici6n del crecimiento de los hongos, tal
como se muestra en las fotografias anexas, en contraste, los testigos mostraron un
desarrollo normal de los hongos en cada caso.
Trichoderma
Respecto a Ia respuesta de las bacterias a los tratamientos ensayados, se detect6 que
E!Winia sp no tuvo crecimiento y para el caso de Ia bacterias Pseudomonas sp. y
Xanthomonas sp. se registr6 un efecto bacteriostatico, habiendo una mejor acci6n a dosis
altas y menor conforme baja Ia concentraci6n.
Las ultimas lecturas realizadas el 29 de noviembre de 2010, continuaron con Ia misma
tendencia en los resultados especificados anteriormente.
25 Aniversario C•encra y Tecnologia para elCampo IVlexrcano
Campo Experimental Bajio
Km. 6.5 Carretera Celaya-San Miguel de Allende. Mpio. Celaya. Guanajuato
C.P. 38110. AP 112. Tel. (461) 611 5323. Fax: (461) 611 5431
www.inifap.gob.mx
\
lnstituto Nacional de Investigaciones
Forestales, Agrlcolas y Pecuarias
"20I 0, Aiio de Ia Patria. Bicentcnario dcllnido de Ia
lndcpcndcncia y Ccntcnario del Inicio de Ia Hcvoluci6n".
Conclusiones
En base a los resultados obtenidos en los bioensayos realizados se concluye:
1. Las dosis evaluadas del producto BlueCore BI0-3107 inhibieron el crecimiento de
los hongos Phytophthora capsici, Rhizoctonia so/ani, Fusarium sp y Trichoderma sp.
2. Las dosis evaluadas del producto BlueCore BI0-3107 inhibieron el crecimiento de
Ia bacteria Erwinia sp.
3. El producto BlueCore BI0-3107 mostr6 una mejor acci6n a dosis altas y menor a
baja concentraci6n para el caso de las bacterias Pseudomonas sp. y Xanthomonas sp.
Anexo: Observe el crecimiento de los hongos en el testigo, segun el case, en Ia primera caja
Petri, en contraste con el nulo crecimiento de los tratamientos evaluados.
Phytophthora capsici
Rhizoctonia solani
Trichoderman sp.
Fusarium sp.
25 Aniversario C1encoa y Tec,..ooog•a
paroJ el Campo Mex•cano
Campo Experimental Bajio
Km. 6.5 Carretera Celaya-SanMiguel de Allende. Mpio. Celaya. Guanajuato
C.P. 38110. AP. 112. Tel. (461) 611 5323. Fax: (461) 611 5431
www.inifap.gob.mx
INIFAP National Institute of Forestry, Agriculture and Livestock Research Campo Experimental Bajio Km. 6.5 Carretera Celaya-­‐San Miguel de Allende Mpio. Celaya, Guanajuato, C.P. 38110. AP. 112. Tel. (461) 611 5323 Fax. (461) 611 5431 www. Inifap.gob.mx November 29, 2010 [Redacted for Protection] By this document I inform you about the results obtained from the biotests performed with the product called BlueCore Bio-­‐3107 when applied to the fungi Phytophthora capsici, Rhizoctonia solani, Fusarium sp, and Trichoderma sp, and the bacteria Erwinia sp, Pseudomonas sp and Xanthomonas sp. Material submited for biotest: BlueCore Bio-­‐3107 Type of biotest: Assessment of the product BlueCore Bio-­‐3107 placed in a culture media made of Papa-­‐Dextrose-­‐
Agar and its response to the fungi Phytophthora capsici, Rhizoctonia solani, Fusarium sp, and Trichoderma sp, and the bacteria Erwinia sp, Pseudomonas sp and Xanthomonas sp. Methodology: The product BlueCore Bio-­‐3107 was placed in a PDA (papa-­‐dextrose-­‐agar) culture media. The treatments shown in Table 1 were applied at the time of emptying the culture media in Petri dishes at a temperature of 30 to 40 oC. Once the different doses were applied to the culture media, they were left still to cool for solidification. Once the culture media solidified, the Petri dishes were labeled and then the fungi Phytophthora capsici, Rhizoctonia solani, Fusarium sp, and Trichoderma sp, and the bacteria Erwinia sp, Pseudomonas sp and Xanthomonas sp were individually planted. Once sown, they all were submitted to incubation at 25 oC during 15 days (the biotests started on October 26, 2010). Daily observations were made, and in order to verify the behavior of the involved microorganisms in contact with the product BlueCore Bio-­‐3107, the attached photographs were taken on November 9, 2010. Further observations were made until November 29, 2010. Page 1 of 4 Table 1. Biotests of Doses of product BlueCore Bio-­‐3107 applied to PDA media in Petri dishes to assess their response against fungi Phytophthora capsici , Rhizoctonia solani , Fusarium sp , and Trichoderma sp , and the bacteria Erwinia sp , Pseudomonas sp and Xanthomonas sp. Celaya, Gto. 2010 Dose / Petri TREATMENTS dish 1. BlueCore Bio-­‐3107: 200 ml of product in 10 L of water 0.5 ml 2. BlueCore Bio-­‐3107: 400 ml of product in 10 L of water 1.0 ml 3. BlueCore Bio-­‐3107: 752 ml of product in 10 L of water 1.88 ml 4. BlueCore Bio-­‐3107: 1,000 ml of product in 10 L of water 2.50 ml 5. BlueCore Bio-­‐3107: 1,200 ml of product in 10 L of water 3.00 ml 6. NO product in Petri dish -­‐-­‐-­‐ Results: Following observations made daily during the duration of the biotests, the ensuing results were obtained: In the case of the fungi, the Phytophthora capsici, Rhizoctonia solani, Fusarium sp, and Trichoderma sp were subjected to the treatment of the BlueCore Bio-­‐3107 as specified in the Table 1 and the results show that the growth of the fungi was inhibited in all cases as it is shown in the attached pictures, while, in contrast, all non-­‐treated Petri dishes show a normal fungi development. In the case of the bacteria the response shows that the Erwinia sp did not develop, while the bacteria Pseudomonas sp and Xanthomonas sp recorded a bacteriostatic effect, obtaining better results at highr doses and lower as the concentration diminishes. The last observations carried out during November 29, 2015 continued with the same trend observed in the previous results. Page 2 of 4 Conclusions: Following the results obtained from the biotests the conclusions are: 1. The doses assessed of the product BlueCore Bio-­‐3107 inhibited the growth of the fungi Phytophthora capsici, Rhizoctonia solani, Fusarium sp, and Trichoderma sp. 2. The doses assessed of the product BlueCore Bio-­‐3107 inhibited the growth of the bacteria Erwinia sp. 3. The product BlueCore Bio-­‐3107 showed a better action at high doses and worse at lower concentrations in the case of the bacteria Pseudomonas sp and Xanthomonas sp. Addendum: Note the growth of the fungi in the non-­‐treated first Petri dish versus the zero growth in the ones treated. [Four sets of pictures of Petri dishes] Page 3 of 4 Note the growth of the bacteria in the non-­‐treated first Petri dish versus the zero growth in the ones treated in the case of the Erwinia sp and the different results in the treated Petri dishes depending on the concentration in the case of the bacterias Pseudomonas sp and Xanthomonas sp. [Four sets of pictures of Petri dishes] Signed Engineer Abraham Jiménez Camargo Phytopathology Laboratory of the C.E. Bajío [Two signatures] Page 4 of 4