National Research Centre of Plant Biotechnology

FINAL TECHNICAL REPORT
Construction of an Integrated Database on
Biosafety and use of GMOs in India
Principal Investigator :
Dr. T. R. Sharma
Coordinator : Prof. K.R. Koundal
Co- Investigators:
Dr. K. C. Bansal
Dr. N. K. Singh
Dr. Kishore Gaikwad
National Research Center on Plant Biotechnology
IARI, New Delhi-110012
www.nrcpb.org Email: [email protected]
Objectives of the Project
•Construction and Updation of the Database
•Retrieval, storage and management of data on biosafety
of GMOs
•To make GM Crops database public through a dedicated
Web site
•To impart training to the Scientists and Technical Staff
on Biosafety web resources
Relational Diagram Showing Relationship Among Different Tables
Basic Flow Chart of the NRCPB Database
Enter query
Authentication
Outputs
Inputs
Query
COMPUTE-SERVER
WEB-SERVER
(SUN V240)
(SUN ULTRA10)
QUERY INTERFACE
Results
JAVA - JDBC
MySQL
Promo
ter
Crop
Env_
haz
MySQL
Health_
haz
Soil_
micr
Develo
-per
APPL-SERVER
JSP ENGINE
USER INTERFACE
Primary
Database
(Transgenics)
MySQL
Gene
MIDDLE WARE
Primary
Database
(GM_Pub)
HTML – DHTML FLASH
www.nrcpb.org
GM Crop Database
Availability of Database on WWW
www.nrcpb.org
Click here to
access GM crop
database
GM Crop Database Home Page
GM Crop Search Database Interface
GM Crop Search Database Interface
Transgenic Line Query Interface
Transgenic Line Query Interface
Result Page for the Query Submitted
Transgene-Crop-Promoter Query Interface
Result Page for the Query Submitted
Promoter Info Query Interface
Result Page for the Query Submitted
Developer Institution Query Interface
Result Page for the Query Submitted
Current status of information stored in transgenics
database
Crop
Cotton
Number of
transgenic line
Number of
genes
Number of
promoter
Developer
137*
4**
1***
20
Rice
4^
5^^
2^^^
1
* Brinjal
-
1%
1***
1
* Tomato
-
1#
1***
1
* Potato
-
2&
2
&&
1
* Chickpea
* Corn
-
1+
1***
1
-
3^^^^
-
-
* 58 Bt cotton hybrids approved for commercial planting in India till date,
rest are released for field trials in different zones.
** (cry1Ac,cry1Ab,cry2Ab,Vip3A), *** (CaMV 35S)
^ (SGR1, SGR2, LLRICE06, LLRICE62), ^^ (Psy, crtI, lyc, Zea mays psy, Bar)
^^^ (Ubi-1 promoter, GT1 promoter), ^^^^ (cry1F,cry1X, CP4EPSPS)
# (Rep gene)
& (Ama1, RGA2), && (CaMV 35S, GBSS)
+ (Cry1Aa3), % (Cry1Ab)
* Transgenic crops under development and field trials
GM Publication Query Interface
Result Page for the Query Submitted
Result Page for the Query Submitted
Result Page for the Query Submitted
Current status of information stored in
GM-Publication Database- June 2007
Year
2006-07
2005
2004
2003
2002
2001
2000
1999
1998
1997
Total
Biosaf
Environ
Food Risks&
Soil Select
ety &
ment &
Soy
& Benefit
Bt Cotton Rice
Brinjal Tomato Corn Potato Canola Micro able Others
IPR
Health
bean
Feed
s
flora Marker
Issues
Concern
2
14
3
5
3
4
7
2
1
0
41
5
10
7
6
5
5
1
0
0
39
6
4
10
8
8
3
1
0
1
41
6
6
10
3
8
4
1
0
1
39
7
10
13
4
5
8
3
3
1
54
4
2
6
6
5
6
0
0
1
30
5
2
1
3
4
1
2
2
2
22
40
25
26
20
13
12
14
7
5
0
162
6
4
0
1
0
0
0
0
0
0
11
7
7
7
8
2
3
2
3
5
2
46
28
15
1
1
0
1
1
0
2
1
50
17
13
13
9
8
9
7
3
2
0
81
19
12
10
8
12
7
5
5
7
1
86
2
8
8
4
3
3
1
0
0
29
3
1
5
1
0
1
1
0
0
12
3
4
8
5
9
9
4
2
1
45
Total number of records = 788
List of Important Users of DB
Total Number of Hits in the Database = 2450
2. National
1.
Indian Agricultural
ResearchResearch
Centre onInstitute
Plant
Biotechnology
3. Mahatma Phule Krishi Vidyapeeth
4. National Bureau of Plant Genetic Resources
5. Tata Energy Research Institute, TERI
6. NRC for Sorghum
7. Jawaharlal Nehru Krishi Vishwa Vidyalaya
8. Tamilnadu Agricultural University
9. Central Food Technological Research Institute
10. Israel Life Science Industry
11. Vasantdada Sugar Institute, Pune
12. Centre for DNA Fingerprinting and Diagnostics
13. Kerala Agricultural University
14. Cornell University
15. Dr.
16.
Central
Babasaheb
Rice Research
Ambedkar
Institute
Marathwada
University
17. Dr.Balasaheb Sawant Konkan Krishi Vidyapeeth
18. Rajasthan Agricultural University, Bikaner
19. Dhaka University
20. Reuters India
21. Central Institute for Cotton Research
1. Crop Design
2. Monsanto
3. Tranzpac
4. Emerging Solutions
22. Sathyabama University
24.
Central Research
23. Osmania
UniversityInstitute For Jute & Allied
Fibres
25. Bishop Heber College, Trichi, TAMILNADU
26. Amity Institute of Biotechnology
27. Birla Institute of Technology
28. University
29.
Ministry of of
Environment
Agriculturaland
Sciences,
Forests BANGALORE
30. Gauhati University
31. University of Agricultural Sciences Dharwad
32. Kerala university
33. Directorate of Rice Research
34. Junagadh Agricultural University
35. Punjab
36.
Sher-e-Kashmir
State Council
University
for Science
of Agricultural
& Technology
Sciences
37. IARI Regional Station, Pusa, Bihar
38. Central
39.
NationalSoil
Research
SalinityCentre
Research
for Rapeseed
Institute
Mustard
40. Central Institute of Freshwater Aquaculture
41. Apeejay institute of Management
42. Delhi University
5. JK-Agrigenetics Ltd
6. GrasslanD Technology
7. MAHYCO
Feedback Query Form
User`s Feedback
Objective 4
National Training Workshops
on
Biosafety Issues and Web Resources in GMOs
Dr. R. K. Katiyar
Course Director &
Director
National Research Centre on Plant
Biotechnology, IARI, New Delhi –110012
Email:[email protected]
Dr. K. R. Koundal
Course Co-Director &
Joint Director Research
Indian Agricultural Research Institute, New Delhi -110012
Email:[email protected]
Dr. T. R. Sharma
Course Coordinator
National Research Centre on Plant
Biotechnology, IARI, New Delhi –110012
Numbers and Period of Trainings
First Training
: April 23-25, 2007
Second Training : May 14-16, 2007
Third Training : June 7-9, 2007
About the Participants and Resource Persons
Participants
Total No. of Participants = 45
ICAR Institutes Represented = 18
SAUs represented = 13
Resource persons
ICAR
DBT
DU
CSIR
SAUs
ICRISAT
MOEF
BCIL
Major Areas Covered in the training
•Biosafety issues Related to GMOs
•GM crops development and their post release management
•GMO detection in food feed and seeds
•Cartagena protocol & safe trans boundary movements of GMOs
•Environmental and health concerns related to GMOs
•Regulatory issues and regulatory agencies
•Availability and use of Web Resources on GMOs etc.
•Practical on Web based Resource Utilization
Training Manuals Developed
A Technical Bulletin
Some Events of the 1 st Training Workshop
Some Events of the 2 nd Training Workshop
Some Events of the 3 rd Training Workshop
Participants Feed Back about the Training
Outcome of the Project
A Database: A web based Comprehensive database on Biosafety Issues related to GMOs
Publications
1. Sharma, T.R. K Srivastava, S.Singh and K.R. Koundal. 2006. Development of an Integrated
Database on Biosafety and use of GMOS in India. Proc. Int Conf Implications of the Cartagena
Protocol on Biosafety, Org by: Ministry Environment and Forests, Govt. of India. New Delhi .169186.
2. Koundal K.R. and T.R. Sharma. 2006. Biosafety concerns associated with GMOs. Proc. Int Conf
Implications of the Cartagena Protocol on Biosafety, Org by: Ministry Environment and Forests,
Govt. of India. New Delhi .83-89
3. Sharma,T.R., K. Srivastava, S. Singh, A. Kumar, K. Gaikwad, K.C. Bansal,
N.K. Singh and K.R. Koundal.2007. Web based Information Resources on GMOs. In CRTAGENA
Potocols on Biosafety:Decisions to Diagnostics.(Eds Ramdhawa et l.2007)NBPGR,New Delhi 179p.
Technical Bulletin
Sharma TR . 2007. GM Crops Database: An Interactive Web Resource. Pp. 16.
Training Manuals
1.Sharma TR, KR Koundal, RK Katiyar 2007. 1st National Training Workshop on Biosafety Issue and Web
Resources. April 23-25.pp68
2.Sharma TR, KR Koundal, RK Katiyar 2007. 2nd National Training Workshop on Biosafety Issue and
Web Resources. May 14-16.pp76.
3.Sharma TR, KR Koundal, RK Katiyar 2007. 3rd National Training Workshop on Biosafety Issue and Web
Resources. June 7-9. pp80
Infrastructure Developed under the Project
•SUN FIRE V240 SERVER SYSTEM : One number
•Storage Data Workstations : Two numbers.
Developed Trained Human Resource in Bioinformatics
Name of the personnel
Period
Present position
Ms Keerti Srivastava
28-3-2005 to 30-9-06
Joined in IT Company
Mr. Ajay Kumar Mehato
1-10-2006 to 30-6-2007
Tomato Genome Seq
Research Associates
Project
Senior Research Fellows
Ms Sangeeta Singh
15-4-2005 to 30-6-2007
Pigeonpea Genome Project
Ms.Charu Singh
2-3-2007 to 30-62007
ICAR Network Project on
Transgenic Crops
Mr. N. Reddy
20-3.2007 to 8-6-2007
Joined in Ph D
Proposed work for the 2nd Phase
Updating the Database.
To develop a Network of Centres for Making
Database in Regional Languages
(Hindi, Punjabi, Tamil, Telgu and Kanada).
Developing a simple version of the database for
the farming community and common public.
Acknowledgements
Prof. K.R. Koundal
Joint Director Research, IARI
Dr. PA Kumar
Project Director, NRCPB
Keerti
Ajay
Sangeeta
Charu
Financial Assistance
Ministry of Env. & Forest, GOI
GEF- World Bank