[ Care and Use ManUal ] Sirocco Protein PreciPitation Plate

[ Care and Use Manual ]
Sirocco Protein Precipitation Plate
Step 2 – Add Crash Solvent
The Sirocco™ plate was designed to improve the automation and throughput of protein precipitation in drug metabolism/ADME/toxicology labs.
Add precipitation solvent to all wells. Use a ratio of at least 3:1 solvent
The Sirocco Protein Precipitation Plate is a unique combination of novel
to sample. Acetonitrile is the recommended precipitation solvent. The
96-well plate tip design with proprietary new filter membranes.
Sirocco plate is designed to prevent solvent from leaking through the
plate at this step.
A straightforward 7-step procedure designed to enable precipitate free
Step 3 – Load Sample
sample filtrates suitable for high-throughput LC/MS analysis.
Add 20-200 µL of sample (plasma, urine, etc.) rapidly into the wells. Rapid
delivery of sample into the crash solvent creates mixing and initiates
precipitation process.
Step 1 – Preparation
Place the Sirocco Plate on a collection plate for processing. This protects
the valve tips during the processing steps.
Sirocco Protein
Precipitation Plate
96-Well Collection Plate
Sirocco Protein Precipitation Plate
1
[ Care and Use Manual ]
Step 4 – Seal
Step 6 – Filter
Apply vented cap mat, using a cap roller to ensure secure, uniform sealing.
After mixing, place the Sirocco plate on a Vacuum Manifold (e.g. Waters
This will prevent splashing and cross-talk during mixing.
vacuum manifold P/N 186001831), and apply 8-5” Hg (271-508 mbar)
of vacuum for up to three minutes. A higher vacuum setting can be used if
flow does not occur at 10” Hg.
Cap Mat
Note: The Sirocco proprietary valve tip design prevents liquid flow through
the device until a minimum 5” Hg (169 mbar) of vacuum is applied.
Step 5 – Mix
Note: The Sirocco Plate can be filtered by using centrifiguation (@ 2,000 x g
Shake (mix) the loaded Sirocco plate/collection plate assembly on a vortexer
for 1 min.) in place of vacuum device.
(e.g., Multi-Tube Vortexer from VWR) at medium setting (e.g., setting 5 on a
1-10 scale) for 0.5 to 1.0 minute.
Note: There is no need to remove the vented cap mats or valve tips from the
Step 7– Analysis
Sirocco Plate before filtration. This eliminates the risk of cross-talk between
Discard the Sirocco Plate and remove the collection plate containing clear
wells. The valves and vented cap mat are designed to open under vacuum
filtrate from the vacuum device, seal and transfer to the LC/MS autosam-
to allow controlled flow during filtration.
pler or store for later analysis.
Note: Excessive mixing at too high a setting (e.g., setting 8 on a 1-10
Note: There is no need to remove cap mats or valve tips from Sirocco Plate.
scale) could cause cross-talk. Under mixing (e.g., setting 2 on a 1-10 scale)
The Sirocco Plate is discarded after filtration.
will cause clogging or a cloudy filtrate.
Xevo™ TQ MS ACQUITY® SM BSM
Sirocco Protein Precipitation Plate
2
[ Care and Use Manual ]
Sales Offices
Ordering Information
Description
Sirocco Protein Precipitation Plate
(includes 1 plate and 1 cap mat)
Sirocco Protein Precipitation Plate
(includes 5 plates and 5 cap mats)
Volume
Qty/Pkg
Part Number
–
1/pkg
186003873
–
5/pkg
186002448
Austria and European Export
(Central South Eastern
Europe, CIS and Middle East)
43 1 877 18 07
Italy 39 02 265 0983
Australia 61 2 9933 1777
Mexico 52 55 5524 7636
Belgium 32 2 726 1000
The Netherlands
31 76 508 7200
Brazil 55 11 5094-3788
Canada 1 800 252 4752 x2205
China 86 21 6879 5888
CIS/Russia
+497 727 4490/290 9737
Japan 81 3 3471 7191
Korea 82 2 6300 4800
Norway 47 6 384 60 50
Poland 48 22 6393000
Puerto Rico 1 787 747 8445
Singapore 65 6593 7100
Czech Republic
420 2 617 1 1384
Spain 34 936 009 300
Denmark 45 46 59 8080
Switzerland 41 56 676 70 00
Finland 09 5659 6288
Taiwan 886 2 2543 1898
France 33 1 30 48 72 00
United Kingdom
44 208 238 6100
Germany 49 6196 400600
Hong Kong 852 2964 1800
Hungary 36 1 350 5086
India and India Subcontinent
91 80 2837 1900
Sweden 46 8 555 11 500
All other countries:
Waters Corporation U.S.A.
1 508 478 2000
1 800 252 4752
www.waters.com
Ireland 353 1 448 1500
© 2010 Waters Corporation. Waters, The Science of What’s Possible, Xevo and ACQUITY are trademarks of Waters Corporation.
Pall,
, and Sirocco are trademarks of Pall Corporation.
VW R is a trademark of VW R International.
August 2010 720003377EN Rev B KK-PDF
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Waters Corporation
34 Maple Street
Milford, MA 01757 U.S.A.
T: 1 508 478 2000
F: 1 508 872 1990
www.waters.com