AgarClave User`s Guide Page 1 AGARCLAVE 60 + 60

Transcription

AgarClave
User's Guide
AGARCLAVE
AUTOCLAVE FOR THE PREPARATION OF CULTURE MEDIA
AGARCLAVE 60 + 60 LITRES WITH CENTRAL CLOUSURE
TKA Teknolabo A.S.S.I. Via Quattro Strade 2 LACCHIARELLA (MI)
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AgarClave
User's Guide
Page 2
Dear Customer,
we thank you for having chosen once again one of our products. We kindly ask you to properly fill this
guarantee and send it back to us.
Certificate of guarantee N.
Date of purchase
Product Code 755
Serial Number
Lot N.
Check 1
Check 2
Check 3
Check 4
Registration data of the customer
Corporate name
Address
ZIP.
City
Get in contact with
Tel.
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AgarClave
User's Guide
Page 3
DESCRIPTION:
This unit has been studied in order to give an answer to the needing of those who have to produce
culture media.
The AgarClave serie is available in several models (10, 20, 40, 60, 2x20, 2x40, 2x60) basically
differing in the useful volume of the sterilising well/wells.
The control of the sterilising cycle is done by a microprocessor electronic card.
The shaking speed of the culture medium is obtained using two pairs of metallic propellers adjustable
in height. Their rotation is obtained thru the application of a rotating magnetic field on a magnetic rotor
to which the propellers are linked. This magnetic linking avoids any possible leaking and is extremely
easy to maintain. The shaking speed of the culture medium is largely adjustable.
An alpha-numerical display gives to the operator information about the temperature and the status of
the cycle.
Up to 15 different sterilising programs can be set, with the possibility of inserting an after sterilisation
constant temperature "cooking phase" for the addition of additives not bearing ordinary sterilising
temperature.
The building characteristics of this autoclave allow an easy and complete cleaning of the apparatus,
which is very important considering the product to be handed.
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AgarClave
User's Guide
The autoclave is provided with security systems independent from the circuit of cycle control:
Pic. 1
- mechanic security valve on sterilising well (Pic. 1 ref.1)
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AgarClave
User's Guide
Pic. 2
- mechanic security valve on heating jacket (Pic. 2 ref.1)
- security thermostat for heating jacket (Pic. 2 ref.2)
-
security pressure switch on heating jacket directly intervening
on exahust (outlet) electrovalve (Pic. 2 ref.3)
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AgarClave
User's Guide
AgarClave 5 litres
Code 755005T
Page 6
Autoclave for sterilisation of culture media with double jacket
Technical Specifications
European Norms
Type
Working Capacity
External Frame
Sterilisation Chamber
Internal Vessel
Settable Parameters
Culture Homogenisation
Recirculation pump for jacket
Supplements
Control of the Sterilising Cycle
Temperature Accuracy
Sterilising Programs
Temperature probes
Safety devices
Cooling
Printer/Recorder
Power Supply
Power Consumption
Working Temperature
Working Humidity
Packaging Dimensions
Packaging Weight
CEE EN 61010, CEE EN 61000-3-2, CEE EN 60601-1-2
Indirect heating with double jacket (steam generator and sterilisation chamber)
5 litres of culture media
Enamelled steel
AISI 316L stainless steel. Internal dimensions 150 x 354 mm (∅ x h)
Removable
¾ Sterilisation temperature 90°C to 121°C (194°F to 250°F)
¾ Sterilisation time from 1 to 99 min.
¾ Adding of supplements/additives after the sterilisation 40°C to 90°C (86°F to 194°F),
¾ Distribution temperature: 40°C to 80°C (86°F to 176°F), step of 1°C
Mechanical shaker with rotating propellers, with variable speed to get the best
homogeneity of the components (20 to 250 rpm)
Included
Inlet for supplement adding
By microprocessor
± 0.5°C at 121°C (250°F)
Up to 15, (sterilisation time and temperature), with the possibility of inserting/excluding
the supplement addition
Two probes model PT100 (one in the work trays, the other in the steam generator)
¾ Automatic safety overpressure valve (working vessel)
¾ Automatic safety overpressure valve (jacket)
¾ Safety thermostat
¾ Safety over pressure switch
Automatic thru softned tap water
Included
230 V, 50/60 Hz; ± 10%. Other power supplies on request
2,3 kW
+15°C to +40°C (59°F to 104°F)
10% to 85% RH
450 x 602 x 900 mm (W x D x H) approx.
70 Kg approx.
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AgarClave
User's Guide
AgarClave 10 litres
Code 755010T
Page 7
European Norms
Type
Working Capacity
External Frame
Internal Vessel
Settable Parameters
Supplements
Temperature probes
Safety devices
Cooling
Printer/Recorder
Power Supply
Power Consumption
Working Temperature
Working Humidity
Packaging Weight
Enamelled steel
Removable
Included
By microprocessor
± 0.5°C at 121°C (250°F)
Included
230 V, 50/60 Hz; ± 10%. Other power supplies on request
3,3 kW
+15°C to +40°C (59°F to 104°F)
10% to 85% RH
85 Kg approx.
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AgarClave
User's Guide
AgarClave 20 litres
Code 755020T
Page 8
European Norms
Type
Working Capacity
External Frame
Internal Vessel
Settable Parameters
Supplements
Temperature probes
Safety devices
Cooling
Printer/Recorder
Power Supply
Power Consumption
Working Temperature
Working Humidity
Packaging Weight
Enamelled steel
Fixed
Included
By microprocessor
± 0.5°C at 121°C (250°F)
Included
380 V, 50/60 Hz; 3ph+N+GN; ± 10%. Other power supplies on request
7,5 kW
+15°C to +40°C (59°F to 104°F)
10% to 85% RH
105 Kg approx.
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AgarClave
User's Guide
Code 75502x20T
AgarClave 2x20 litres
Page 9
European Norms
Type
Working Capacity
External Frame
Internal Vessel
Settable Parameters
Supplements
Temperature probes
Safety devices
Cooling
Printer/Recorder
Power Supply
Power Consumption
Working Temperature
Working Humidity
Packaging Weight
2x20 litres of culture media
Enamelled steel
Fixed
Included
By microprocessor
± 0.5°C at 121°C (250°F)
Included
15 kW
+15°C to +40°C (59°F to 104°F)
10% to 85% RH
200 Kg approx.
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AgarClave
User's Guide
AgarClave 40 litres
Code 755040T
Page 10
European Norms
Type
Working Capacity
External Frame
Internal Vessel
Settable Parameters
Supplements
Temperature probes
Safety devices
Cooling
Printer/Recorder
Power Supply
Power Consumption
Working Temperature
Working Humidity
Packaging Weight
Enamelled steel
Fixed
Included
By microprocessor
± 0.5°C at 121°C (250°F)
Included
9 kW
+15°C to +40°C (59°F to 104°F)
10% to 85% RH
145 Kg approx.
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AgarClave
User's Guide
Code 75502x40T
Page 11
European Norms
Type
Working Capacity
External Frame
Internal Vessel
Settable Parameters
Supplements
Temperature probes
Safety devices
Cooling
Printer/Recorder
Power Supply
Power Consumption
Working Temperature
Working Humidity
Packaging Weight
Enamelled steel
Fixed
Included
By microprocessor
± 0.5°C at 121°C (250°F)
Included
18 kW
+15°C to +40°C (59°F to 104°F)
10% to 85% RH
250 Kg approx.
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AgarClave
User's Guide
AgarClave 60 litres
Code 755060T
Page 12
European Norms
Type
Working Capacity
External Frame
Internal Vessel
Settable Parameters
Supplements
Temperature probes
Safety devices
Cooling
Printer/Recorder
Power Supply
Power Consumption
Working Temperature
Working Humidity
Packaging Weight
Enamelled steel
Fixed
Included
By microprocessor
± 0.5°C at 121°C (250°F)
Included
12 kW
+15°C to +40°C (59°F to 104°F)
10% to 85% RH
180 Kg approx.
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AgarClave
User's Guide
Code 75502x60T
Page 13
European Norms
Type
Working Capacity
External Frame
Internal Vessel
Settable Parameters
Supplements
Temperature probes
Safety devices
Cooling
Printer/Recorder
Power Supply
Power Consumption
Working Temperature
Working Humidity
Packaging Weight
Enamelled steel
Fixed
Included
By microprocessor
± 0.5°C at 121°C (250°F)
Included
24 kW
+15°C to +40°C (59°F to 104°F)
10% to 85% RH
330 Kg approx.
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User's Guide
Page 14
INSTALLATION:
Place the unit far from heat-sources and excessive humidity, taking care of leaving 10 cm distance
from lateral walls.
Connect the unit to the water net after inserting a proper decalcification group thru nipple 1 in Pic. 3
and connect an outlet line to nipple 2 in Pic. 3, blocking the pipe with a ring.
Pic. 3
Warning: there is hot water and/or steam exiting by nipple 2, use then appropriate pipes, interposing,
when possible, a thermical trap.
Insert the electrical plug, after having checked that the characteristics of the suit the ones on the label.
It is important to make sure that the network is earthing provided and that the earthing is properly
working.
Do not use extensions or multiple shunts to feed this instrument.
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PROGRAMMING:
Pic. 4
Switch on the unit using the main switch button (Pic. 4 ref. 1)
All the inserting or numerical data requesting functions are shown on the alpha-numerical display (Pic.
4 ref.2) by the > symbol on the left of the field to be inserted or corrected.
In the inserting field a value is generally proposed: this value can be accepted or corrected as you
please; the C button cancels one by one the present numbers starting from the one on the farthest
right (last entered), the E button confirms the value shown on the display;
Example:
Presented value 1246, desired value 1346.
Push C button, > 124 is shown
Push 3 button, > 13 is shown
Confirm the value using the E button.
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Push the PROGRAM button, "Program # > " is displayed, using the numerical buttons set the number
between 0 and 14 corresponding to the program to be inserted or corrected.
Once you confirm it "Steril temp. > " is displayed, this refers to the sterilising temperature, then insert
a value between 90 and 121 degrees.
Once you confirm it "Steril temp. > " is displayed, this refers to the requested sterilising time, then
insert a value between 1 and 60 minutes.
Once you confirm it "Final temp. >
(erogating temperature).
" is displayed, this refers to the final temperature to keep
If the set sterilising temperature is higher than 100 degrees, when confirming the "Adv. temp. > "
writing is shown, referring to the temperature the additives have to be added at, insert a value between
30 and the previously setted sterilising temperature value (if this is not required, set as a value the
same final temperature to be kept).
When confirming "Adtv time > " is displayed, referring to the required "cooking" time, insert a value
between 1 and 60 minutes.
When confirming the "final temp. > " is displayed, referring to the final temperature to be kept
(erogating temperature).
Once this last parameter has been inserted the program is permanently kept in memory until a
modificationt takes place, even if there is no power supply.
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USE:
Before opening the well, make sure there is no pressure in the jacket (Pic. 5 ref. 2) and in the well (Pic.
6 ref. 1), otherwise release the pressure in the well thru the tap (Pic. 6 ref. 1) located on the cover of
the well itself, and release the pressure in the jacket switching on the apparatus for at least 20
seconds.
Warning: before performing the above operations make sure the autoclave has completely cooled off
at the end of the previous cycle.
Pic. 5
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User's Guide
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Pic. 6
Opening the well:
Before proceeding check that the main feeding button is OFF.
The instructions herebelow refer to the models with screw knobs cover locking system. For the
opening and closing of the models with cover central locking system please follow the instructions:
-opening: rotate the central handwheel counter clockwise and rotate the locking bar in a convenient
direction to access the working vessel.
- closing: operate in the opposite direction. Please note that it is not necessary to apply
an high rotating torque to obtain a proper closing as the gasket is double lip type.
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Disconnect the connector which connects the autoclave to the lid of the well only for the models where
the temperature probe is hooked to the lid (all the models where the removable sterilisazion basked is
required) or those where the automatic exhaust option has been required.
Disconnect the nipple connecting the autoclave to the cover of the well (Pic. 7 ref.1-5)
Pic. 7
Unscrew and remove the cover itself placing it on a surface, taking care not to damage the probeappendices or the shaker rod.
Warning: for the models with removable baskets the well is subjected to the idrostatic pressure of the
jacket, so when it is empty, if there is some water in the jacket (as it should be) it tends to float.
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Pic. 8
Make sure the space washer of the shating system is in good conditions (its thickness should not be
less than 1 mm), and put bake in its place the shaking rotor making sure it moves freely.
Add the ingredients into the well (Pic. 8 ref. 1)
Put the cover back in its place checking that the O-RING (Pic. 8 ref.2) is properly situated in its own
place and block the cover using the 5 knobs (Pic. 7) taking care of screwing the knobs following the
order:1-3-5-2-4.
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Pic. 9
Check that the erogation nipple is closed (Pic. 9)
Switch on the unit using the main switch button (Pic. 4 ref. 1)
Push start button to start the sterilising cycle, insert the number corresponding to the program to be
performed and confirm it pressing E.
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The sterilising cycle is internally divided into 10 phases (some programs avoid some phases).
Picture 10 shows these 10 phases
Pic. 11
The passage from a phase to the following one happens automatically, but when there are operations
to be performed manualy, in this case the operator is alerted by a buzzer.
It is possible to avoid manually some phases intervening on F1 button, but this opportunity has to be
used only if it is compatible with a good performance of the cycle itself.
Warning: In case of lack of electricity, the unit starts again automatically as the electricity comes back,
therefore if a cycle has been launched, it will automatically restart.
If you want to stop it, press the stop button that puts the autoclave in stand-by.
In some of these cases it is advisable to stop the cycle, to restart it and pressing F1 bring it to a phase
compatible with the gained temperatures.
During the sterilising cycle the buzzer informs the operator when the well reaches a 96/97 degrees
temperature, at this temperature make sure to close the exhausting tap on the cover of the well (Pic. 6
ref. 1).
The operation has to be confirmed pressing the exhaust button.
This operation is not necessary where the automatic exhaust option has been required.
Afterwards the buzzer rings when the temperature for the addition of the thermolabile additives has
been reached (cooking), if it has been previously set (see Programming).
The operation has to be confirmed using the exhaust button.
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At the end of the cycle, when the erogation temperature is reached, a buzzer alerts the operator. To
confirm that the warning has been noticed press the exhaust button.
At this point the reached temperature is indefinitely kept.
Once the drawing of the product is ended, press the sop button.
Warning: each time the operator press the stop the cycle is taken back to phase 0. If you want to
start/restart from a different phase press F1 button to select the required phase.
Information about the status of the autoclave are shown on the alpha-numerical display (Pic. 4 ref.2.)
(i.e. jacket and well temperatures, electrovalves status and so on).
Printer/Recorder of the thermical phase
The preparator of the media is equipped with ALPHAPANEL printer/recorder which gives information
regarding the temperature of the sterilizing well, the time of the beginning and ending of the cycle and
any switch off of the equipment due to lack of power supply or voluntary action.
Simply press the OPEN button (“key”) to start the recorder, once it is on the led is lit. Use the same
button to switch it off. Press FEED to adjust the paper as desired.
When the printing tape has reached the end and you want to re-use it, simply use your finger to rewind
it (the tape is located in the internal right side).
The recorder displays the day, month, year and hour. These parameters have been previously set by
the manufacturer and they do not need to be modified. If a modification is required press OPEN before
switching on the autoclave and release the button after pressing the main green switch. The ID unit
serial number will appear on the display. Confirm this value using the “E” enter button and the year,
month, day, hour, minute will appear on the display. If you need to replace the given values, use the
“C” (cancel) button, insert the new values and confirm every single operation with “E” enter. The last
thing on the display will be enter code>0. Confirm the 0 value with “E” to reach the operative stand-by
phase.
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Programmed delayed start
You have the possibility to automatically start the cycle with a delay even if the operator is not
available. The delay is shown in hour/minutes. To set this option follow this procedure:
-
press Select (instead of Start)
insert the number of the program and press E
insert the hour (0…99)
insert the minutes (0…59)
confirm with E, and the new time will appear for few seconds.
The instrument stays on stand-by visualizing the clock, and at the preset time the cycle will start.
Automatic exhaust
This option makes completely automatic the preparation of the culture media. It is very important to
make sure that the duct of the automatic exhaust is sterile. To achieve this make sure to install a
proper hydrophobic bacterial filter, that can be sterilized in line (for ex. PALL C2PFRP1) on the
exhaust side of the valve .
To activate it, it is necessary to install this valve in its place using the supplied clamp pipe and link the
connector of its excitation coil to the predisposed plug on the control head. Make sure the valve is
properly installed with the arrow facing up. The valve itself can be sterilized and we strongly
recommend this procedure if you operate in adverse conditions and /or with particularly sensitive
media. In this case the sterilisation will be done with the bacterial filter installed on the ventilation
pipeholder.
The preparators equipped with this option can be used following the manual exhaust procedure. To do
this simply replace the exhaust with the supplied clamp cap to close the door and follow the ordinary
procedure. The system can automatically recognise whether the exhaust has been installed.
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Acid pump (optional)
The function of this pump is to introduce a certain amount of acid into the steam generator jacket at
the end of every cycle to dissolve any possible lime deposit in the jacket itself. The amount of acid is
defined by the manufacturer (TKA). The acid solution to be used is composed by citric acid 30%.
WARNING: the control system cannot verify that during the phase of the introduction of the
acid the acid itself is pumped into the jacked as there is no connection between the autoclave
and the vessel with the diluted acid.
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Cleaning:
Pic. 11
At the end of each production make sure to clean carefully the autoclave.
For the instrument with removable well:
-
Open the well in the way described in chapter "Use" .
Remove it in order to make the cleaning operation easier.
Before putting it back check that the probes for the control of the water level in the jacket (Pic. 12
ref. 1 and 2) are in vertical position and that the O-RING (Pic. 12 ref. 3) is properly situated in its
own place.
For the instrument with fixed well introduce hot water (at least 50°) into the well itself, start the shaker
for a few minutes and, at the same time, release completly the water.
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Page 27
TROUBLESHOOTING
These are the most probable cases as well as the action to be taken.
For different problems please refer to our technical staff.
Possible cause
The lamp of the main switch
does not light when the main
switch is pressed
Suggested action
1. Defective main power switch
2. Improper plugging of the
power cord
3. Power cord disconnected
4. Main fuses interrupted
Replace the switch
Plug it in properly
The
display
shows
no
information when the main
switch is turned on
1. Broken transformer
2. Defective KT-OUT board
3. Broken pressostat
Replace the transformer
Replace the board
Replace the pressostat
The acid pump does not work
starting a program (in case this
option is available)
1. Broken acid pump
Replace the acid pump
The shaker does not work
1. The regulation knob is set for
speed equal to zero
2. The washer of the shaker is
too worn-out
3. Broken control circuit
4. Broken transmission belt
5. Broken motor reducer
Turn the know clockwise
Replace the cord
Replace the fuses
Replace the washer
Replace the broken parts
Replace the belt
Replace the motor reducer
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Possible cause
Suggested action
The in/out water valves are
continuously on
1. The in-tap water is closed
2. The level sensor are broken
or disconnected
Open the in-tap water
Reconnect or replace the water
level sensors
The in-out water valves are on
during the warming up
1. The exhaust valve is dirty
2. The jacket safety valve is
dirty
Clean or replace it
Replace it
The plotter/printer does not work
(in case this option is available)
1. The plotter/printer has not
been activated
2. The power supply of the
plotter/printer is broken
Press the “OPEN” key
1. The thermostatic switch is
broken
2. The warming system does
not work properly
Replace the thermostatic switch
The sterilization temperature is
never reached
3. The exhaust valve or the
safety valve are dirty
The indicated temperature is
incorrect
1. The IO-STERO board is not
tuned
2. The PT100 probe is broken
3. The PT100 holder is broken
Replace the board
Replace the broken parts (solid
state switch/heating
resistences)
Replace the defective parts
Call the service
Change the probe
Change the probe holder
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Possible cause
Suggested action
During the cooling the difference
between the working vessel and
the jacket is more than 40C
(during the cooling a difference
up to 35-40C can be normal)
1. The recirculation pump is
broken
Replace the recirculation pump
Air evacuation is insufficient, or
there is no evacuation (only if the
automatic exhaust valve is
available)
1. Defective automatic exhaust
valve
2. The automatic exhaust valve
has not been connected
Replace the valve
Pressure does not increase
(although temperature is close to
100C)
1. Imperfect closing
exhaust valve
Close completely (if manual) or
clean/replace the automatic
valve (if automatic)
Replace the valve
of
the
2. Defective jacket pressure
safety valve
3. Defective pressure gauge
4. Leakage in pipe or other part
Connect it properly
Replace the pressure gauge
Repair the part
Steam leakage through the lid
gasket
1. Worn our lid gasket
Replace the lid gasket
“CHECK SAFETY SW” display
1. Safety thermostat broken
Replace the thermostat
The media preparator switches
off during the cycle
1. Overpressure into the jacket
2. Pressure switch broken
Call the service
Replace the pressure switch
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Page 30
MAINTENANCE:
In addition to the normal cleaning operations no particular maintenance is ordinary required, anyway it
is necessary to remove any lime deposit to properly maintain the heating elements.
To better achive this use soft water and, periodially, acording to the toughness of the water, introduce
citric acid in the jacket.
Before the beginning of each cyle, as previously described, make sure that the space washer of the
shating system is in good conditions (its thickness should not be less than 1 mm).
If it is weared out replace it.
Picture 13 shows some devices placed into the autoclave
Pic. 13
1-fusibles
2-relay for the thermostat
3-pump for the circulation (only for a few models)
4-inlet water electrovalve
5-outlet water electrovalve
6-inlet tap
7-jacket security valve
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WARRANTY:
All our apparatus have been carefully tested and are covered by a 6 months warranty starting from the date of purchasing.
TEKNOLABO A.S.S.I s.r.l. undertakes to freely repair or replace those parts that within waranty period Teknolabo itself should consider not efficient.
The complete replacement of the apparatus and the extension of the warranty after an operation due to a failure are excluded.
TEKNOLABO A.S.S.I. is not responsible for possible direct or undirect damages caused to people or things by failures of the apparatus or depending on
forced suspension in its use.
The warranty does not cover: aesthetic parts, damages caused by careless, bad or improper use or bad installation, mistaken connection to tension
different from the one suitable to the apparatus, or different from the limitations fixed by CEI rules (+ -10% nominal value).
Possible in laboratory-repairing is performed in our Technical Assistance Centre situated in Via Quattro Strade 2, - Lacchiarella - Milano unless different
EXPLICIT indication or authorisation of the constructor.
The warranty declines if the apparatus is tampered or repaired by nunauthorised personnel.
The apparatus considered defective will have to be sent to us together with a copy of the certificate of warranty.
The expenses and the risks of the travelling from and to our laboratory will be charged by the customer.
In case of field repairing (to be performed only on constructor's not arguable decision), the customer has to pay the calling-right as a repayment for the
travelling of the personnel.
Validity of the warranty
The waranty is valid only if the coupon has been fulfilled and has been sent to Teknolabo A.S.S.I. within 8 days from the date of the unit reception.
Any dispute should occurr the Court of Pavia is competent.
TEKNOLABO A.S.S.I.
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AgarClave
User's Guide
WIRING DIAGRAM
5 -- 10 LITRES (1 PH)
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AgarClave
User's Guide
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AgarClave
User's Guide
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AgarClave
User's Guide
WIRING DIAGRAM
10 -- 20 LITRES (3 PH)
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AgarClave
User's Guide
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Page 36
AgarClave
User's Guide
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Page 37
AgarClave
User's Guide
WIRING DIAGRAM
40 -- 60 LITRES (1 PH)
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AgarClave
User's Guide
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Page 39
AgarClave
User's Guide
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Page 40
AgarClave
User's Guide
HOOKUP DIAGRAM SINGLE CHAMBER
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Page 41
AgarClave
User's Guide
HOOKUP DIAGRAM DOUBLE CHAMBER
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Page 42

AgarClave User`s Guide Page 1 AGARCLAVE 60 + 60

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