Protexin - Avian AquaSolve Ltd

Probiotics International
Protexin Concentrate for Broilers
Probiotics International
Somerset, UK
1989: Company formed.
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Cattle and sheep products.
1996: Became part of Tithebarn Group.
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UK animal feed supplement manufacturer.
Compressed salt blocks.
Family owned since 1935.
1997: Probiotics of other species.
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Agriculture.
Companion Animals.
Humans.
Aquaculture.
2010: New production site.
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5 km move, retaining skilled work force.
2011: Second production unit.
2012: Queens Award for Export
2013: Continued growth
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Medium size family owned company.
90+ employees.
Production to GMP standards in new purpose
built facilities.
Selling into 50+ countries.
Markets for Agricultural Products
•
South America
Brazil, Argentina, Bolivia, Chile.
•
Sub Continent
India, Bangladesh, Pakistan, Sri Lanka.
•
Asia
Japan, Australia, Thailand, Malaysia, Vietnam, Indonesia,
Philippines, South Korea, New Zealand, China
•
Middle East
Iran, Turkey, Saudi Arabia, Egypt, Jordan, Iraq.
•
Africa
South Africa, Namibia, Mozambique, Kenya, Zimbabwe , Zambia.
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European Union
United Kingdom and the other 26 countries of the EU.
60,000,000 broilers per month fed Protexin Concentrate in Brazil
Major Markets
Why use a Probiotic
Animal performance
growth rates, FCR, egg production, egg fertility.
Animal health
reduced mortality, disease incidence and severity, boost to immune
system, competitive exclusion of pathogens. Gut function
Alleviation of stress
heat stress, dietary changes --- any stress factors.
Modern farm management techniques.
Antibiotic resistance
therapeutic antibiotics for animals
resistance problems for human medicine
Sales and marketing
quality and welfare branding of finished products
export market requirements
International trials evidence to support claims for Protexin for all animals
How do Probiotics work?
1: Competitive exclusion
Attachment sites and for nutrients.
2: Reduction in intestinal pH
Production of lactic acid
3: Modulation of the immune system
Innate immunity
Innate immunity receptors, Toll-like receptors
Lactic Acid
Adaptive immunity
T-helper cells
Modulate balance of Th1, Th2 and Th17 derived cytokines
Mucosal antigen-specific IgA
Adequate production dependent on normal homeostasis of
microflora
What is Protexin Concentrate
 Highly concentrated probiotic - Enterococcus faecium
2 x 109 CFU/g (2 x 1012 CFU/kg)
 Given in-feed or via drinking water or milk (Spray)
 Microencapsulated
Pelleting at up to 900C
Acid tolerant to pH 2.0
Shelf Life
 No Withdrawal Periods
Protexin Concentrate – Poultry
•
•
Daily usage
Group
g/tonne
Age
Broiler - Starter
150
Days 1 to 21
Broiler - Grower
100
Days 22 to 35
Broiler - Finisher
50
Days 35+
Benefits
Improved FCR and DLWG
Reduces the incidence of pathogens
Reduces the effects of stress
Increased egg production
Protexin effect on broiler performance and immune
response when compared to other Growth Promoters
Afshin Zakeri and Pedram Kashefi1 – The Comparative Effects of Five Growth
Promoters on Broiler Chickens Humoral Immunity and Performance – Journal of
Animal and Veterinary Advances 10 (9):1097-1101, 2011
1 Poultry
Section, Department of Animal Science, Faculty of Science, Islamic Azad University,
Tabriz Branch, Tabriz, Iran.
Comparative effect of Protexin
Materials and methods.
• 100 day old broilers per treatment, Cobb 500.
• 42 day feeding period.
Treatment Groups
Control
Probiotic
Prebiotic
Antibiotic
Organic Acid
Plant Extract
Without GP
Protexin
MOS
Avilamycin
Nutracid Acid
Vitacel
@ 100 g/t
@ 1000 g/t
@ 100 g/t
@ 800 g/t
@ 4 kg/t
Recording
• Weight Gain, FCR, Feed Intake and EFPE
• HI titre, before and days 7 and 14 after vaccination against Newcastle disease
Comparative effect of Protexin
Weight Gain
EFPE takes account of changes in :1. Age
2. Mortality
3. Weight Gain
4. FCR
2450
2400
2350
2300
2250
2200
2150
2100
2050
2000
EFPE
360
349
350
Control
Protexin
MOS
Avilamycin Nutracid
Focus
Vitacel
342
340
335
330
320
310
FCR
315
309
308
300
2.3
Control
2.2
2.1
2
1.9
1.8
1.7
1.6
Control
Protexin
MOS
Avilamycin
Nutracid
Focus
Vitacel
Protexin
MOS
Avilamycin Nutracid
Focus
Vitacel
Protexin Concentrate v MOS. Broilers
•
Commercial Trial Brazil, 2010
•
Material and Methods
MOS Group
Protexin Group
•
Feeding period 46 Days
•
Recording
Slaughter weight
Daily liveweight gain
Feed conversion rate
Production Index
Economic analysis
596000 broilers
229000 broilers
23 groups
10 groups
Protexin Concentrate v MOS. Broilers
MOS
(+ve control)
Protexin
Concentrate
Average age at slaughter, days
46.1
46.0
Slaughter weight, kgs
2.75
2.77
Daily liveweight gain, g
59.71
60.19
Feed conversion rate
1.711
1.679
1.675 to 1.75
1.65 to 1.695
0.075
0.045
321.5
329.8 +8.3
Physical Performance
Range in FCR
Production Index (EFPE)
Financial Comparison
Protexin
Concentrate
MOS
Slaughter weight, kgs
Feed conversion rate
2.75
1.711
1.679
Feed consumed, kg
4.70525
4.61725
Cost of rations, R$
0.502
0.504
Broilers slaughtered per day
Ration costs, R$
Additional margin, R$
$
450000
1,062,915.90
1,047,503.96
R$ 15,151.90
$8755
Protexin Concentrate v MOS. Broilers
Conclusions
• the performance of broilers fed with Protexin Concentrate was better
than those consuming MOS (slaughter weight, daily gain, feed
conversion and production index).
• From the economic standpoint it is beneficial to use Protexin
Concentrate in broiler rations.
• All groups treated with Protexin Concentrate were negative for
salmonella at slaughter.
Effect of Protexin on broiler performance and
development
Belchiolina Beatriz Fonseca 1, Marcelo Emílio Beletti 2, Max Siqueira da Silva 3, Paulo Lourenςo
da Silva 3, Ivamario Nahas Duarte 2 and Daise Aparedida Rossi 3 – Microbiota of cecum, ileum
morphometry, pH of the crop and performance of broiler chicks supplemented with
probiotics. – R. Bras. Zootec. Vol. 39 no. 8 Viςosa Aug. 2010
1
Programa de Pós-Graduaςão em Imunologia e Parasitologia Aplicadas da Universidade Federal de Uberlândia, Brazil.
2 Instituto de Ciências Biomédicas da Universidade Federal de Uberlândia, Brazil.
3 Laboratório de Biotecnologia Animal Aplicada, Faculdade de Medicina Veterinária da Universidade Federal de Uberlândia.
Effect of Protexin on broiler performance and development
Material and Method
120 day old broiler chicks, Cobb-Vantress.
Treatment period
28 days
Treatments
Group A = Control
Group B = Protexin @ 150 g/t
Recording
Days 1, 7, 12, 18, 23 and 28.
pH of crop and enterobacteria count in cecum
Body weight
Effect of Protexin on broiler performance and development
Table 1: pH of Crop
Day
Control
Protexin
1
6.80 a
4.98 b
7
4.39 a
2.98 b
12
3.37 a
3.06 a
18
5.71 a
3.88 b
23
4.58 a
4.11 a
28
4.86 a
4.55 a
Pathogenic bacteria reach the digestive system of
birds by passing through the acid barrier of the crop.
High Lactobacillus counts and low pH in the crop
reduces the incidence of potentially pathogenic
bacteria such as Salmonella passing into the intestinal
tract.
The low pH in this study could have contributed to
The general health of these birds.
Values with different superscripts are significantly different p<0.05
8
7
Control
6
Protexin
5
4
3
2
1
0
1
7
12
18
23
28
Effect of Protexin on broiler performance and development
Table 2 : Enterobacteria Counts CFU/g
Day
Control
Protexin
% Reduction
7
1.7 x 109 a
7.7 x 108 b
54.7
12
5.6 x 109 a
2.2 x 109 a
60.7
18
1.4 x 109 a
2.3 x 108 b
83.6
23
8.3 x 108 a
6.4 x 107 b
92.3
28
1.4 x 108 a
2.6 x 106 b
98.1
Values with different superscripts are significantly different p<0.05
There is a vast number of undesirable
microbiota in the cecum of normal
chickens and these specifically are from
the family Enterobacteriaceae
which includes such organisms as
Salmonella.
Enterobacteria can cause damage to
intestinal cells and can become
pathogenic if conditions are suitable for
their development
Effect of Protexin on broiler performance and development
Villi height μm
2000
1800
1600
Control
1400
Protexin
1200
1000
800
600
400
200
0
1
7
12
18
23
28
Control
233
466
500
950
900
1083
Protexin
267
667
1267
1433
1700
1767
Age in Days
Effect of Protexin on broiler performance and development
Table 3: Weight of birds
Day
Control
Protexin
Diff
1
45.42
45.79
+ 0.37
7
127.49
138.69
+ 11.2
12
280.00
281.8
+ 1.80
18
490.24
501.64
+ 11.4
23
828.33
859.34
+ 31.0
28
1265.60
1330.73
+ 65.1
Other benefits seen in this trial.
•
•
•
Quicker and lower reduction of
crop pH levels.
Lower potential pathogen
counts
Better intestinal development
seen as improved villi
development
Effect of Protexin on broiler performance and development
Conclusion
Probiotics reduce the pH of the crop contents contributing to a decrease in
enterobacteria in the cecum. Length of villi increased which can lead to better feed
absorption.
Belchiolina Beatriz Fonseca, Marcelo Emílio Beletti, Max Siqueira da Silva, Paulo Lourenςo
da Silva, Ivamario Nahas Duarte and Daise Aparedida Rossi.
Microbiota of cecum, ileum morphometry, pH of the crop and performance of broiler chicks
supplemented with probiotics.
R. Bras. Zootec. Vol. 39 no. 8 Viςosa Aug. 2010
Effect of Protexin and alternative on bone strength
and structure
H Ziaie1, M Bashtani2, M A Karimi Torshizi3, H Naeeimipour,2 H Farhangfar1 and A Zeinali4 – Effect
of Antibiotic and its alternatives on Morphometric Characteristics, Mineral Content and Bone
Strength of Tibia in Ross Broiler Chickens – Global Veterinaria 7 (4): 315-322, 2011
1 Research
Centre of Agriculture and Natural Resources, South Khorasan, Birjand, Iran.
2 Dept. of Animal Sciences, Birjand University, Birjand, Iran.
3 Dept. of Animal Science, Faculty of Agriculture, Tarbiat Modares University, Tehran, Iran.
4 Dept. of Animal Science, Faculty of Agriculture, Ferdowsi University of Mashhad, Mashhad Iran.
Comparative effect of Protexin
Materials and methods.
• 360 day old broilers, Ross 308.
• 42 day feeding period.
•
Treatment Groups
Control
Antibiotic
Probiotic
Prebiotic
Plant Extract
Organic Acid
•
Without GP
Virginiamycin
Protexin
MOS (Immnuwall)
Digestrom
Propionic acid
@ 15ppm
@ 150 mg/kg
@ 1000 mg/kg
@ 450 mg/kg
@ 400 mg/kg
Recording
Bone Weight, thickness, weights and mineral contents
Elasticity and yield stress
Blood Ca and P
Comparative effect of Protexin
Tibia Bone parameters at 42 Days
Control
Antibiotic
Protexin
Prebiotic
Bone weight, g
4.42b
5.10a
5.25a
5.14a
5.09a
5.10a
Length, mm
88.19b
91.53a
91.72a
91.02a
91.86a
91.28a
Thickness of inner wall, mm
1.41b
1.72a
1.73a
1.71a
1.73a
1.68a
Thickness of outer wall, mm
2.53b
2.88a
3.10a
2.91a
2.96a
2.89a
Medullary canal diameter, mm
5.33a
4.58b
4.57b
4.54b
4.56b
4.62b
a,b
Means with different superscripts within the same row differ significantly (P<0.05)
Plant Extract Organic Acid
Comparative effect of Protexin
Blood Analysis at 42 Days
Control
Antibiotic
Protexin
Prebiotic
Calcium, mg/dl
13.82c
16.11b
17.63a
16.50a
16.50ab
16.50ab
Phosphorous, mg/dl
2.32b
4.69a
5.15a
4.84a
4.93a
4.43a
a,b
Plant Extract Organic Acid
Means with different superscripts within the same row differ significantly (P<0.05)
Calcium, mg/dl
Phosphorus, mg/dl
19.00
6.00
5.50
5.00
4.50
4.00
3.50
3.00
2.50
2.00
1.50
1.00
18.00
17.00
16.00
15.00
14.00
13.00
12.00
11.00
10.00
Control Antibiotic Protexin Prebiotic
Plant
Extract
Organic
Acid
Control Antibiotic Protexin Prebiotic
Plant
Extract
Organic
Acid
The effect of Protexin Concentrate to control
Salmonella Minnesota in the chicken gut.
Profa E Santin1 – Protexin Concentrate adicionado na ração para o controle de
Salmonella Minnesota em frangos de corte. – Private communication, March 2011
(publication 2011/12 tbc)
1 Departamento de
Medicina de Veterinaria, Universidade Federal Do Parana, Curitiba, SP, Brazil
Protexin for Salmonella Control
Materials and methods.
• 60 broilers, 20 per treatment
Treatments
• T1 = Negative Control
• T2 = Positive Control
• T3 = Protexin Concentrate
without Salmonella inoculation
with Salmonella inoculation
with Salmonella inoculation
Birds inoculates orally with 108 Salmonella Minnesota on day 15
Trial period 35 days
Protexin Concentrate given in feed at recommended rates
Protexin for Salmonella Control
Salmonella in Litter
Day 21
Day 35
Negative Control
0.00 ± 0.0a
0.00 ± 0.0a
Positive Control
19952 ± 1.2b
3981 ± 1.7c
Protexin
794 ± 19.9b
707 ± 3.6b
Salmonella in cloacal swabs
48 hours post inoculation
Negative Control
0.00 ± 0.00a
Positive Control
8912.2 ± 173.8b
Protexin
28.2 + 42.7 – 28.2a
Salmonella counts
Crop
Cecum
Negative Control
0.00 ± 0.00a
0.00 ± 0.00a
Positive Control
7 ± 3b
19952 ± 19054c
Protexin
5 ± 5b
204 ± 40.7b
% Reductions
Litter day 21
Litter day 35
Swabs after
48hrs
Cecum day 35
96.0%
82.2%
99.7%
99.0%
a – c Values with different superscripts show statistical differences (P<0.05)
Thank you for your attention
Any questions??
Manufacturing
Manufacturing Process
•
Steps 1 to 4 Fermentation
•
Step 5 Concentration
Cells harvested by gentle filtration NOT centrifuged
Almost 100% recovery compared to 50 to 60% when centrifuged
•
Step 6 Micro-encapsulation
THE PROTEXIN UNIQUE STAGE
Micro-encapsulation using cryoprotectants
Proteic and Disaccharidic Complex
•
Step 7 Freeze drying (Lyophilisation)
Frozen quickly to -40’C
Bacterial structure maintained
•
Step 8
Adding other ingredients depending on
product type
Quality Control
Sampling and testing
• Viability
• Stability, including long term
• Contamination
Protexin LifeStart
and Concentrate
is for all farms not
just organic ones
Protexin
Compatibility
Recovery of Protexin at differing pelleting
temperatures
110.00
105.00
% Recovery
100.00
95.00
80oC
90oC
95oC
90.00
85.00
80.00
75.00
Start
1 Minute
2 Minutes
3 Minutes
4 Minutes
5 Minutes
Acid Stability within Gastrointestinal Tract
(unbuffered in vitro growth pH2 for 2 hours)
1.00E+12
1.00E+11
Bifidobacterium
bifidum
Lactobacillus
acidophilus
Lactobacillus
bulgaricus
Lactobacillus
plantarum
Lactobacillus
rhamnosus
Streptococcus
thermophilus
Enterococcus
faecium
Average
1.00E+10
1.00E+09
1.00E+08
pH 6.8
pH 2.0
Protexin Stability, % of original count
110.00
100.00
Sample 1
Sample 2
Count for
Specification
2 x 109 CFU/g
90.00
80.00
70.00
60.00
50.00
Start
3 Months
6 Months
9 months
12 Months
15 Months
18 Months
21 Months
24 months
Protexin Stability, Compatibility with Chlorine
1.00E+09
1.00E+08
1.00E+07
1.00E+06
1.00E+05
1.00E+04
0 ppm
1.00E+03
2 ppm
4 ppm
1.00E+02
6 ppm
8 ppm
1.00E+01
10 ppm
1.00E+00
0 hours
2 hours
4 hours
8 hours
10 hours
12 hours
Protexin Concentrate