Platelet count by village

Isola&
geografici
e
marcatori
di
rischio
trombo&co
C.L.
Balduini
(Pavia)
[email protected]
Ogliastra
Ogliastra
10 villages
genealogical information dating
back to 17th century
538 pedigrees (on average 4.6
generations deep)
high endogamy (from 70% to
90%)
Fulvio Roiter, 1955
Marianne Sin-Pfaltzer: Baunei, ritratto di donne, 1955
Marianne Sin-Pfaltzer: Baunei, ritratto di donna, 1955
Ogliastra
10 villages
genealogical information dating
back to 17th century
high endogamy
(from 70% to 90%)
12,517 enrolled people (80% of
resident population)
538 pedigrees (on average 4.6
generations deep)
12,517 enrolled people (80% of
resident population)
64 pedegrees with more than one
member with thrombocytopenia
platelets x 109/L
Platelet
count
by
village
Northern Ogliastra
Western Ogliastra
Remainders
Platelets
x
109/L
Platelet
count
by
age
classes
age
class
Prevalence
of
thrombocytopenia
and
thrombocytosis
by
age
classes
12
PLT>400
<18
0
0
1
1
2
2
3
3
4
4
5
5
%
6
%
6
7
7
8
8
9
9
10
10
11
11
12
PLT<150
18-39
40-59
>=60
Age class
Northern Ogliastra
Western Ogliastra
<18
18-39
40-59
>=60
Age class
Remainders
Northern Ogliastra
Western Ogliastra
Remainders
In conclusion:
● platelet count is a highly heritable trait
● platelet count is higher in women than in men
●
platelet number declines with ageing
●
the propensity to present platelet counts slightly higher than
400x109/L or lower than 150x109/L is a new genetic trait.
Haematologica 2011;96:96-101
Rela&onship
of
MPV
versus
PLT
count
in
Ogliastra
popula&on
Pearson’s
r=‐0.36,
p<0.0001
Rela&onship
of
platelet
parameters
with
aging
in
Ogliastra
popula&on
Distribu&on
of
MPV
in
Ogliastra
popula&on
Distribu&on
of
MPV
in
Ogliastra
popula&on
Men
Women
Age,
y
43.8
(21.4)*
45.7
(21.0)
PLT,
x109/L
240.5
(65.3)*
260.3
(67.5)
8.9
(0.9)*
9.2
(1.0)
High
total
cholesterol,
n
(%)
1015
(21.0)
1374
(21.9)
Low
HDL‐C,
n
(%)
1173
(24.4)†
1924
(30.7)
223
(4.6)
317
(5.1)
MPV,
fL
Characteris&cs
of
the
11,085
Ogliastra
inhabitants
enrolled
in
the
study
Dyslipidemia
treatment,
n
(%)
At
least
1
pregnancy,
n
(%)
3875
(61.8)
Oral
contracep&ves,
n
(%)
1028
(16.4)
Diabetes,
n
(%)
372
(7.7)†
368
(5.9)
Varicose
veins,
n
(%)
129
(2.7)†
871
(13.9)
Hypertension,
n
(%)
1902
(39.5)†
1934
(30.9)
An&hypertensive
treatment,
n
(%)
730
(15.2)
1029
(16.4)
Obesity,
n
(%)
705
(14.6)
933
(14.9)
Wine
drinking,
n
(%)
3648
(75.7)†
2855
(45.5)
Cigare7e
smoking,
n
(%)
2698
(56.0)†
1410
(22.5)
Arterial
thrombosis,
n
(%)
281
(5.8)†
207
(3.3)
Venous
thrombosis,
n
(%)
40
(0.83)†
128
(2.0)
!"#$%&$'()*+)&,$-('.(,)&-
!"#
!"#$%&$'()*+)
!"
Thrombo&c
events
by
groups
of
villages
*
Chi
square
test
for
homogeneity
of
proporEons
in
Ogliastra
villages,
p<0.05
both
in
males
and
females
Mul&ple
logis&c
regression
of
arterial
and
venous
thrombosis
on
platelet
parameters
Arterial
thrombosis
Venous
thrombosis
Odds
RaEo
p
Value
Odds
RaEo
p
Value
PLT
1.0
0.573
1.0
0.926
MPV
1.0
0.767
0.9
0.281
Two
separate
models
were
run
for
arterial
and
venous
thrombosis
respecEvely,
each
adjusEng
for
age,
sex,
group
of
villages
and
risk
factors
related
to
thrombosis
Mul&ple
logis&c
regression
for
arterial
thrombosis
**
**
**
**
*
**
**
**p<0.01
*p<0.05
*
**
Odds
RaEo
Mul&ple
logis&c
regression
for
venous
thrombosis
**
**p<0.01
*p<0.05
**
*
**
**
*
Odds
RaEo
Rela&onship
of
mean
platelet
volume
with
&me
since
thrombosis
Western Ogliastra
Remainders
12
10
9
8
7
0
10
20
30
40
50
60
0
10
20
30
Northern inner Ogliastra
12
11
MPV, fL
MPV, fL
11
10
9
8
7
0
10
20
30
40
50
Years since trombotic event
60
40
50
60
Rela&onship
of
mean
platelet
volume
with
&me
since
thrombosis
all
thromboses
artherial
thromboses
Western
Ogliastra
Northern‐inner
Ogliastra
Remainders
("&
("%
("$
("#
("*
(")
(
!"(
!"!
!"'
!"&
!"%
!"$
!"#
+
)
*
#
!"#$%&%'()"&"*"(+
$
%
Conclusions
● Mean
Platelet
Volume
is
not
a
risk
factor
for
thrombosis
●
The
increased
MPV
previously
described
in
subjects
with
acute
thrombosis
was
a
consequence
instead
of
a
cause
of
thrombosis
Conclusions
● Mean
Platelet
Volume
is
not
a
risk
factor
for
thrombosis
●
The
increased
MPV
previously
described
in
subjects
with
acute
thrombosis
was
a
consequence
instead
of
a
cause
of
thrombosis
●
A
cross‐sec&onal
study
in
the
general
popula&on
of
a
gene&c
isolate
confirmed
several
well
known
risk
factors
for
thrombosis
I
da1
presenta1
sono
sta1
o8enu1
grazie
alla
collaborazione
di:
Ginevra
Biino*†
Mario
Pirastu*†
*
Ins1tute
of
Popula1on
Gene1cs,
Na1onal
Council
of
Research,
Sassari,
Italy
†
Shardna
Life
Sciences,
Cagliari,
Italy