Diapositiva 1

Produzione di Idrogeno
mediante microorganismi
fotosintetici.
G. Torzillo
Istituto per lo Studio degli Ecosistemi
Sede di Firenze
Energia e idrogeno: Le esperienze e le strategie europee. Il nuovo bando
energia del 7PQ. Le iniziative in Italia. Milano, 12 luglio, 2012
Metabolic pathways in Chlamydomonas reinhardtii
- Gaffron and Rubin, 1942
Le nostre principali attività di ricerca svolte nell’ambito
del progetto Hydrobio (FISR, MIUR, 2004-2009), EBH2
(Regione Toscana)
1) Selezione di ceppi di
Chlamydomonas reinhardtii con
elevati tassi di produzione di H2
2) Ottimizzazione delle
condizioni colturali
3) Verifica all’aperto in
fotobioreattori
PsbA gene – D1 protein
153
294
(Courtesy of U. Johanningmeier)
D1
Chlamydomonas reinhardtii mutants screened for the H2 production at ISE-FI
1) Photobioreactor;2) Intermediate bottle for gas-liquid conversion;3) Liquidaccumulating bottle; 4) Electronic balance; 5) PC with interface for data recording and
storage; 6) - 7) - 8) Probes: Temp, pH, Eh; pO2; PAM-2100; 9) Magnetic mixing system.
a)
c)
b)
d)
Produzione di H2 mediante batteri fotosintetici
Brodi colturali arricchiti con acqua di vegetazione, quale sottoprodotto
dell’industria olearia , sono stati utilizzati per la produzione di energie verdi
Brodi colturali arricchiti con acqua di scarico, sono stati utilizzati per la
produzione d’idrogeno. In collaborazione CNR-ISE- UNIFI ( Prof Roberto
De Philippis).
2H2O
PE =
(8 photons)
O2 + 4
e-
+ 4H
+
H2ase
2H2
237 KJ/mole H2 x 2 moles
(209KJ/ mole quanta in PAR) x (8 moles of photons)
= 28.3%
28.3 x 0.45 = 12.7% (of solar light)
An ultimate potential objective might be to increase BioHydrogen
production as close as possible to about 600,000 m3 ha-1 y-1, which
represents solar light conversion efficiency to H2 of about 10% in sunny
areas.
1) Torzillo G., Scoma A., Faraloni C., Ena A., Johanningmeier U. (2009). Increased hydrogen photoproduction by means of a sulfur-deprived Chlamydomonas reinhardtii D1 protein
mutant. Int J Hydrogen Energy: 34: 4529-4536.
2) Giannelli L., Scoma A., Torzillo G. (2009). Interplay between light intensity, chlorophyll concentration and culture mixing on the hydrogen production in sulfur-deprived C. reinhardtii
cultures grown in laboratory photobioreactors. Biotechnol Bioeng104: 76-90.
3) Faraloni C., Torzillo G. (2010) Phenotypic characterization and hydrogen production in Chlamydomonas reinhardtii QB binding D1 protein mutants under sulphur starvation: changes
in chlorophyll fluorescence and pigment composition. J Phycol, 46: 788-799.
4) Torzillo G. Giannelli L., Martinez-Roldan A.J., Verdone N., De Filippis P., Scarsella M., Bravi M. (2010). Microalgal culturing in thin-layer photobioreactors. Chem Eng Trans , 20: 265270.
5) Torri C., Samorì C., Adamiano A., Fabbri D., Fartaloni C., Torzillo G. (2011). Preliminary investigation on the production of fluels and bio-char from Chlamydomonas reinhardtii
biomass residue after bio-hydrogen production. Biotechl. Biores. (in press) Doi 10.1016/j.biortech.2011.01.064.
6) Faraloni C., Ena A., Pintucci C., Torzillo G. (2011). Enhanced hydrogen production by means of sulfur-deprived Chlamydomonas reinhardtii cultures grown in pre-treated olive mill
wastwater. Int. J. Hydrogen Energy 36: 5920-5931.
7) Scoma A., Giannelli L Faraloni C., Torzillo G. (2011). Outdoor H2 production in a 50-liter tubular photobioreactor by means of a sulfur-deprived culture of the microalga
Chlamydomonas reinhardtii . J. Biotechnol. (in press). Doi: 10.1016/j.jbiotec.2011.06.040
8) Scoma A., Giannelli L Faraloni C., Torzillo G. (2011) Sustained H2 production in a Chlamydomonas. reinhardii D1 protein mutant. J. Biotechnol. (in press). Doi:
10.1016/j.jbiotec.2011.06.019
9) Scarsella. M., Torzillo G., Cicci A., Belotti G., De Filippis P., Bravi M. (2011). Mechanical stress tolerance of two microalgae. Process Biochemistry (in press). Doi:
10.1016/j.procbio.2011.07.002
10) Carlozzi P. (2009). The effect of irradiance growing on hydrogen photoevolution and on the kinetic growth in Rhodopseudomonas palustris, strain 42OL. Int. J. Hydrogen Energy,
34(19): 7949-7958.
11) Carlozzi P., Lambardi M. (2009). Fed-batch operation for bio-H2 production by Rhodopseudomonas palustris (strain 42 OL). Renewable Energy, 34: 2577-2584.
12) Carlozzi P., Buccioni A., Minieri S., Pushparaj B., Piccardi R., Ena A., Pintucci C. (2010). Production of bio-fuels (hydrogen and lipids) through a photofermentation process. Biores.
Technol. 101: 3115-3120.
13) Carlozzi P, Scoma A, Pintucci C, Ena A. (2010). Co-production of bioH2 and biomasses rich in oil from two Rhodopseudomonas palustris strains: 42OL and 6A. In Clean Energy:
Resources, Production and Developments. Ed: Aiden M. Harris, Nova Science Publishers Inc, New York, Chap. 13, pp. 163-178, ISBN: 978-1-61761-509-2
14) Carlozzi P., Pintucci C., Piccardi R., Buccioni A., Minieri S., Lambardi M. (2010). Green energy from Rhodopseudomonas palustris grown at low to high irradiance values, under fedbatch operational conditions. Biotechnol. Lett. 32: 477-481.
15)Rodolfi L, Chini Zittelli G, Bassi N, Padovani N, Biondi N, Bonini G, Tredici M R (2009). Microalgae for oil: strain selection, induction of lipid synthesis and outdoor mass cultivation in
a low-cost photobioreactor. Biotechnol Bioeng 102: 100-112.
16)Tredici M.R., Chini Zittelli G., Rodolfi L. (2010). “Photobioreactors”. In: Encyclopedia of Industrial Biotechnology: Bioprocess, Bioseparation, and Cell Technology, Vol 6. Flickinger
M.C. (ed). J. Wiley & Sons Inc., New York, pp. 3821-3838.
17) Bondioli P., Della Bella L., Rivolta G., Casini D., Prussi M., Chiaramonti D., Chini Zittelli G., Bassi N., Rodolfi L., Tredici M.R. (2010). Oil production by the marine microalga
Nannochloropsis sp. F&M-M24. Proc. of 18th Europ. Biomass Conf., 3-7 May, Lyon, France. pp. 538-541. ISBN 978-88-89407-56-5; DOI: 10.5071/18thEUBCE2010-VP1.3.53
18) Chini Zittelli G., Rodolfi L., Bassi N., Tredici M.R. (in press) Photobioreactors for microalgae biofuel production. In: "Algae for Biofuels and Energy" (eds M. Borowitzka and N.
Moheimani).
19)Bondioli, L. Della Bella, G. Rivolta, G. Chini Zittelli, N. Bassi, L. Rodolfi, D. Casini, M. Prussi, D. Chiaramonti, M.R. Tredici. Oil production by the marine microalgae Nannochloropsis
sp. F&M-M24 and Tetraselmis suecica F&M-M33. Bioresource Technology (submitted 12.12.2011).
20) Adessi, A., Torzillo G., Baccetti E., De Philippis R. (2012) Susstained outdoor H2 production with Rhodopseudomonas palustris cultures in a 50 L tubular photobioreactor. Int. J. of
Hydroge energy;
21) Carlozzi P. (2012). Hydrogen photoproduction by Rhodopseudomonas palustris 42OL cultured at high irradiance under a semi-continuous regime. J Biomed Biotechnol (in press)
22) Samori C Torri C, Fabbri D, Falini, Faraloni et al., (2012) ChemSus Chem. (in press)
23) Torzillo G. Seibert (2012) Hydrogen production from Microalgae In Richmond ed. 2012 (in press).
24) Torzillo G., Faraloni C., Giannelli L. (2012) Biotechnology of hydrogen production with the microalga C. reinhardtii (The Science of Algal Fuels ( Gordon R., Seckbach eds), 2012
A collaborative research and development (R,D&D) program
created in 1977 on a task-shared “bottom-up” basis
H Y D R O G E N
Task-21:
I M P L E M E N T I N G
A G R E E M E N T
“Bio-Inspired Hydrogen and BioHydrogen”
Subtasks
2010-2015
A. Bio-inspired Systems
B. Dark BioHydrogen Fermentation Systems
C. Basic studies for Light-Driven BioHydrogen
Production
D. Biological Electrochemical Systems
E. Overall Analysis
Michael Seibert, Operating Agent
AN IMPLEMENTING AGREEMENT OF THE
INTERNATIONAL ENERGY AGENCY
IEA
Support
MIUR (HYDROBIO)
Regione Toscana (EBH2; PROSEV;
OLIVARE)
BANCHE (CRF; MPS)
ENEL Brindisi (CO2-capture)
EU: Oli-PHA- European project (20122015) - AQUAGRIS; ALGINET,
GRAZIE
per
l’attenzione
2H2O O2 + H2