Method Li-sting Mon Oct 22 10:16:05 2012 Method: cannabinoids

Method Li-sting Mon Oct 22 10:16:05 2012 Method: cannabinoids

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MS
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wcflstation
Method:
Method
—
Li-sting
Mon Oct
*
* —
22
.
*
* * * * * * * * * *
10:16:05
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2012
cannabinoids—ms
*****——**
**4**4*
**
Notes
This
lea
is
a
using
general detection method for
both ZSD and TSD detectors.
.
*
* *
*
* * *
3B00
of
abuse
in
GO
* * *
* * * *
Module
drugs
Address:
44
Autosampler
8400
Solvent
Sample
Syringe size:
Injection Mode;
Penetrarion Depth:
Penetration Depth:
Default Clean Vial:
Default Clean Volume:
Default Clean Strokes,
Default Clean Drawup Speed,
Clean Mode Pre—Inj Solvent Flushes:
Clean 4ode Post—Inj Solvent Flushes;
Clean Mode Pre—inj Sample Flushes:
C.ean Mode Solvent Scurce:
io
uL
Std Split/Splitless
50 %
90
I
5-0
1
5.0
3
C
0
I
Use Prepahead: no
Selay Time:
COO niz
Table
Valve
Front
Injector
Oven Power:
Temperature:
Time
mm)
On
250
C
Split
State
Initial
0.35
Middle
1177
Type
Split
Ratio
ott
75
off
On
Injector
Type
1079
Oven Power:
Coolant;
Enable Coolant at:
Coolant rineot:
Temp
(C)
Raze
C/min
250
TIme
(min
0
Split
State
On
Ott
250 C
20.00 mln
Hold
fin)
Total
(nr.)
3_CO
3.00
Split
Ratio
;r.izial
Front
Injector
25
EEC
Type
I
Constant Column Flow:
Pulse Pressure:
Pulse Duration,
Middle
Injector
EFC
Type
1.2 ni/mm
10.0 psi
0.05 mm
1
Constant Column Flow:
Pressure Pulse:
1.0 ml/min
none
uL
uL/sec
biological
samo
Column
Oven
Coolant:
Enab]e Coolant at,
Ccolant Timeout;
Stabilization Tne:
Tenp
Rate
(C/mm)
20
2S3
303
Middle
TSD
50.0
Range
yes
Bead
Power
Off
A
Intiar
Front
Attenuation
1
B
Tine
(inn)
Signal
Source
Initial
Front
Port
Autozero
11
Signal
Source
Output
Data
On
300 0
3.100 A
Fast
line
(mm)
Port
3.35
3.15
B.5C
Detector
Initial
Oatput
Total
inn)
0.S5
3.CO
4.95
C.O
Time
(mm)
Port
Hold
flIrt)
25.0
Oven Power;
Temperaturc:
Bead Current:
Time Constant:
Output
Off
50 C
20.00 mm
0.15 nm
Attenuation
1
C
Time
mm)
Signal
Source
Initial
Front
Attenuation
1
Acquisition
Detector Sunch Pate
Xonrtor Length
Front F:D/TSD ScDle:
Midde F:D/TSD Scale:
Rear FrD/TS Scale:
rntegratcn
Parameters
Address
Subtract Blank Baseline
Initial S/IJ Iatio
Initial Peak Width
Initial Tangent Height %
Monitor Noise
Measurement Type
Initial Peak Reject Value
Report Unidcntified Peaks
Report Missing Peaks
Calibratcn
Setup
8 points
5.0 :-z)
64 bunched oornts
1 Volts
1 Volts
1 Volts
:
:
:
:
:
:
:
:
ChanneL
sec)
:idde
No
5
4 sec
10%
Before every run
Peak Area
1000
counts
Yes
No
Address
Calculation Type
Niztber of Calbratron Levels:
Curve Origin
Curve tic
Werghted Regression
Replicate Treatcent
Averagrngweioht
Replicate Tolerance
Out—of—Tolerance Action
Calibration Range Tolerance
Out—of—Tolerance Action
44
(12.8
44
Channel
<iddle
(No CalibratIon
%
I
Fe r cc
Linear
:-Jone
Average Caibratron Replicates
53
applied to new rapLizares
Add replicates within tolerance of
No Action
1O.0
No Action
0.5
Veritication Setup
?ddress
Deviation Tolerance
Out—of— T0.eran7e
Table
Peak
Address
channel
channel M’ddle
Reference Peaks Time Windows:Width:
ether Peaks Time Wndows
Width:
Peak
Time
Events
TabIe
Table
Time
* *
Retention Time
Retentron Time
2.0%
2.0%
44
Table
Channel
Middle
Empty
* * * * * * * * * * * * *
4003
* * * *
0.10 nun0.10 nr..
Empty
Address
Events
Middle
100.3%
o Action
Action
44
44
F4S/GC
*
* *
Module
* *
* * * * * * * * * * *
Address:
*
*
56
Security Options Required:
Instrument Configuration:
None
Internal
Mass Data Type:
Number Cf Segments:
Last Modified:
Liethod Start Time:
Centroid
3
5/29/2012 9:54
0.00 minutes
Segment: I
Punning Tine:
Last Modified:
on r zat I C r.
FIl/MUL ELA?
0.03
2.75 minutes
i0,30/20j’ 11,58 AX
Segnenc: 2
RunnIng Tame:
last Mcdffed:
S€t?OInts
Calibrant:
IRCA Segment
2.75
7.60 minunes
13/18/2011 9:03 AX
1
2
3
4
and ci
AM
—
—
Off
Scan Typo:
Mass Rango:
Tune Type:
Segment
El
Full
40: 550
Auto
Low
Mass
(m/z)
High
Mass
m/z)
10
100
250
400
99
249
399
1000
RE’
Storage
Level
(m/z)
Ion
Time
Factor
(%)
31
35
35
35
:cn±zation Type:
argat iXZC:
!4ax ion Tame:
EmIssion Current:
100
100
100
100
20030 counts
40003 usezonds
10 uaArps
General Parameters:
Scan Mode:
Scars Avera;ed:
Data Pate:
Mass Defect,
SultIplaer Otsot:
Count Threshold:
Normal
6 nrcroscans
U..,,
C
C
Segmont: 3
Running Time:
Last Modified:
Ionization:
2.13
nZ
rnu/lCCu
volts
cholesterol segment
7.80
6.50 minutes
5/29/2012 9:54 AM
Off
—
MS
REPORT
FORMAT METHOD
seconds/scan)
PRINT
OPTICNS:
SncIe—Run
Satple
Reports:
Report:
Prrnted
AsC:
Header
Sample
Sarnpe
Report:
Peport:
Configuration:
Header
Description:
Sample ID
Instrument ID
Acquisition Date
Calculation Date
Sample Notes
mi
Compound
Operator
Last Calibration
Data File
Method
Report;
Printed
Header
Compound
Reports:
No
Analysis
Verification
Calibration
Samples:
Samples:
Samples:
Yes
Yes
Yes
Contiquration:
Header
Descroticn:
Sanple :D
Instrjmenc TD
Measurement Type
Acqu’sitz.cn Date
Calculatacn ate
Sample Type
mi. Sample Notes
Printout
iulti—Run
Scheduling:
after
Sample
List
Reports
RZSZLTS
list
of
of
injection
Column
Summary
Summary
Control
Control
tic
No
No
Report:
Report:
Charts:
Charts:
No
No
No
No
Separator;
separator
in
regional
copies to print:
decimal digits for
io:<AT:
Report:
Report:
Peport:
Reports:
Print
ASCII
Print
Print
Number
Number
each
Reports:
Printed Block
ASCI Block
Prtntcd Curve
Use
Operator
Last Calibration
Calibration ype
ata File
Vethcd
alock Reports:
Ca1bratron
ASCII
No
No
settrngs
amouuts:
3
Results
to
Print:
Target
Compounds
Identified:
railed:
Unidentified
Results
Table
Missing:
Yes
Yes
Yes
TIC:
Unknown:
Dp1icate
Yes
Yes
Yes
Peaks:
Format;
STANDARD
Show
Show
Rut.
Ion Rato Information:
Compound Group Totals:
No
No
Documentation:
Acqusa.t.-on
Informatron:
Run Log:
Error Log:
Calibration Report:
Revision Log:
Sample Notes,
Method Notes,
Segment
No
NO
No
No
No
No
No
CHROMATOGRAM WORMAT:
Target
Compound
Quan
Scan
Time
Chromatogram
Ions;
Function
RIC
Merged
Channels,
Scale:
Start Time:
End Time:
Number
of
Overlap
ATolitude
0.00 minutes
1A40.00 minutes
Plots:
I
Seconds;
3
Scale:
AutoscaTh
Percentage
Plot
Specification:
(Zoom
of
Factor
Offset
=
=
1.00)
5%
Annotation:
General:
Acquisition Segments:
Integration Events 4 Baselines:
Background Correction:
Yes
Yes
Yes
Peak Annotation;
Peak Types
COMPOUrfl
to
REPORTS:
Target
Comccunds:
RetentionTime:
Scan Number:
Compound Name:
Compound Number:
Yes
No
Yes
No
Annotate:
Identified:
Failed:
Missing:
TIC’s:
Unknowns:
Duplicates:
Yes
Yes
Yes
No
No
No
Results
to
Print:
Identificd:
Failed:
Missing:
Yes
Yes
Yes
Results:
Acquisition
Target
Segment
Type:
STANDARD
Info:
Yes
Plots:
Quan
Ion
Unidentified
Results
Chromatogran
Sample Spectrum
Raterence Spectrum
Raw Sample Spectrum
Peaks:
to
Print:
TIC;
Unknown:
Duplicate:
Yes
Yes
Yes
Results:
Acquisition
Library
Searched
Quan
Piots
CALIBRATION
Block
Ion
Not
Segment
Info:
Yes
Crronatograz
uan
or. Chromatoaram
BLOCK
REPORT:
Report:
SCANDARD
Calibration
Block
Report
Report:
L:ST
:nclude
4
Yes
Linear/Linear
Calibration Carves
Report
REPORTS:
Run
Types:
Calibration;
Analysis:
Veriticaticn:
Sort
Sample Soectrur,
First liSrarv Match
Difference
Sample
lzbra ry Searched:
Number of Curves/page:
Show Outliers on Curve:
Curve Scales:
Title:
SAMPLE
STANDARD
Plots;
Type:
Title:
Curve
Type;
Yes
Yes
Yes
By:
First Key:
Group Ry:
Summary
Report:
Tite:
Coupounc
Frst Key
-
Sursary
Report
Summarize:
Retention Time;
Results (Amount):
Area:
Height:
Control
Charts:
Yes
No
No
No
—
Spectrum
1st Match
Title:
Control
Chart
Plot
Retention Time
*/—2 Standard Deviations
Chart:
Limits:
Control
Options
Number
Datahandling
Method
Calculations
Setup
of
Plots/Page:
Section
4
Address
SC
General
Noise Type
Measurement Type
Calibration Type
Unretained Peak Time
Report Missing Peaks
Report Unknown Peaks
Normalize Results
rgnore Calibration Data
Scale Air Flow Samples
Peak To Peak
Area
Internal Std
0.000
mm
Yes
Yes
No
No
No
Compound Confirmation Criteria
Ion Ratio Type
Abs o 1 U to
Qualifier Integration
Quan Ion
PaT
Tolera—--Ce
0.5
Ccnfrrr. RaT
Confirm S/N Threshold
NC
Report Outliers As
railed
CnromatDzran
Prccessncs
Integration
Quan
cns
Scar. Function Channels
Smooth Chrorratosram
Remove Sczkes
Intial Peak Width
Slope Sonsrtiv’ty
InItial Tangent Height
Inrtial Peak ReJect Value
Tzre Events Table
Tine Events Table
Tentative
Library
counts
Event/TIte
Ito
identificatron
Search
No
Reporting
Absolute-.
All
Yes
1.330
Treatment
Calibration
Results
Replicates Addrrion Mode
Replicates Addrtion Rule
1odatecrrpdrable ?arams
Retention Trne
Reference Spectruz
Target
cn ?ataos
Analyss
t1orged
I;o
t:0
4.0
sec
20
10%
2003
Empty
Results Calculation And
RE To Use
Reporting Threshold
Exclude Duplicates
Results
Points
Append
Always
add
Yes
No
-0
Results
Calibration Parse Tolerance
Out—of—Tolerance Act’cn
Verification
23.0%
NC Act:on
Results
Deviation Tolerance
Out—of—Tolerance Action
100.0%
No Action
new
replmcates
Compound
Table
Compound
Table
Empty