WASHINGTON ANIMAL DISEASE DIAGNOSTIC LABORATORY

Transcription

WASHINGTON ANIMAL DISEASE
DIAGNOSTIC LABORATORY
Annual Report
FY 2014
July 1, 2013 through June 30, 2014
Washington Animal Disease Diagnostic Laboratory
Washington State University
College of Veterinary Medicine
Pullman, WA
Main Laboratory
Puyallup, WA
Avian Health and Food Safety Branch Laboratory
WASHINGTON ANIMAL DISEASE DIAGNOSTIC
LABORATORY - ANNUAL REPORT FY2014
SUMMARY OF DIAGNOSTIC LABORATORY WORK
July 1, 2013 – June 30, 2014
TABLE OF CONTENTS
WADDL Mission and Values
WADDL Annual Highlights
Definitions
WADDL Faculty, Staff, and Graduate Students
5-Year Total Accession Information (FY2010 - FY2014)
5-Year Total Laboratory Test Information (FY2010 - FY2014)
WADDL Accessions by State and by WA County
WADDL Budget and Budget Sources
5-Year WADDL Accessions by Lab. Section (FY2010-FY2014)
Total WADDL Tests by Species of Animal (FY2013 - FY2014)
Laboratory Examinations by Lab. Section (FY2013 - FY2014)
Avian Health and Food Safety Laboratory
Pathology
Parasitology
Aquatic Health
Virology
Molecular Diagnostics
Bacteriology
Serology
Immunohistochemistry
Antigen Detection (Infectious Agent)
Toxicology
WADDL Quality System Highlights and Proficiency Tests
Publications
Presentations (Poster Sessions, Letters, Invited
Addresses)
Book Chapters, Books, Book Reviews
Peer-reviewed Journal Articles
2
3
4-8
9
10-11
12
13
14
15-16
17
17
18-24
18
18
19
19
19
20
21
22
23
23
24
25-26
27-35
27-31
31-32
32-35
Mission
Our mission is to provide accurate, state-of-the-art, timely, and cost-effective
diagnostic services, consultation, disease surveillance and outreach in order to
safeguard animal health, the food supply and public health and to contribute to
the academic mission of the University and College of Veterinary Medicine
through discovery, scholarly activity, and education.
Values
As a combined service and educational unit within a land grant University, the
Washington Animal Disease Diagnostic Laboratory (WADDL) occupies a unique
position that links the University to public and private stakeholders. We recognize
and value the importance of our role in providing a positive, responsive interface
between Washington State University and the animal and public health
community, and between our stakeholders and the academic community.
We value collegial service and outreach to our constituents, which include the
practicing veterinary community, the agricultural animal industry, animal and
public health regulatory agencies, companion animal owners, and the general
public. We also place a very high value on quality, both in service to and
communication with our clientele.
In addition to general diagnostics, we endorse and highly value the critical role we
play in surveillance and detection of emerging and exotic diseases, whether
introduced accidentally or deliberately with intention to disrupt the nation’s food
supply. We value our role as a reference laboratory, our role in helping to assure
a safe food supply, our vital contribution to maintaining international trade of
agricultural animal products, our critical role in public health, and our ability to
contribute to the discipline of diagnostic medicine through education and training
of professional veterinary diagnosticians and discovery of new knowledge.
The Washington Animal Disease Diagnostic Laboratory is guided by a commitment
to excellence, and endorses the core values of Washington State University,
including Quality and Excellence, Integrity, Trust and Respect, Discovery,
Innovation and Creativity, Land-Grant Ideals, Diversity and Global Citizenship,
Freedom of Expression, & Stewardship and Accountability.
3
WADDL Highlights
1. Disease Surveillance Testing (Animals Tested):
Aquatic Health Viral Culture screening
9,268
Epizootic hematopoietic necrosis virus Infectious hematopoietic necrosis virus Infectious pancreatic necrosis virus Oncorhynchus masou virus Viral hemorrhagic septicemia virus Infectious salmon anemia virus Spring Viremia of Carp
Aquatic Health Bacterial screening
7,671
Aquatic Health Bacterial Kidney Disease
3,969
Avian influenza
2,714
Bovine anaplasmosis
3,302
Bovine brucellosis
8,504
Bovine leukosis
2,141
Bovine spongiform encephalopathy (BSE)
6,658
Bovine trichomoniasis
2,007
Bovine virus diarrhea virus-PI–cattle
4,708
Caseous lymphadenitis (sheep and goats)
7,812
Johne’s disease (cattle, sheep, goats)
8,422
Leptospirosis (multiple species)
6,897
Mycoplasma ovipneumoniae (bighorn and domestic
sheep)
3,746
Selenium (multiple species)
2,546
Sheep / Goat scrapie
1,528
Trace Element and Metals (multiple species)
1,478
4
Program surveillance testing at WADDL is done through WSU-WADDL programs,
specific state or regional interagency agreements or contracts, and WADDL
participation in the federal laboratory networks for animal health (National Animal
Health Laboratory Network (NAHLN) of the United States Department of
Agriculture), public health (Laboratory Response Network of the Centers of
Disease Control and Prevention) and animal foods and feeds (US Food and Drug
Administration (FDA) Veterinary Response Network (FDA Vet-LIRN).
5
2. New or Continued Surveillance Programs/Contracts/Agreements
 USDA National Animal Health Laboratory Network Disease Surveillance
Program (African Swine Fever, Avian Influenza, Bovine Spongiform
Encephalopathy, Classical Swine Fever, Chronic Wasting Disease, Exotic
Newcastle’s Disease, Foot and Mouth Disease, Infectious Salmon Anemia
Virus, Scrapie)
 FDA-Center of Veterinary Medicine
o Cooperative Agreement for Surveillance of Salmonella Carriage in
Dogs and Cats
o Expanded Testing Methods for Elements and Metals in Animal
Diagnostic Specimens
o Veterinary Investigation and Response Network for Contamination
Events in Animal Foods and Feeds
 Washington Department of Agriculture (WSDA)-WADDL Interagency
Agreement
o Disease Surveillance (Bovine and cervid brucellosis, Porcine
pseudorabies, Bovine trichomoniasis, Bovine tuberculosis)
o Foreign Animal Disease Outbreak Investigation

Washington State Department of Health
o CDC Laboratory Response Network for Bioterrorism
o West Nile Virus Testing Program

Washington Horse Racing Commission Postmortem Examination Program

Aquatic Health
o Nebraska Game and Parks Commission Fish Health Inspection
Program
o New Mexico Fish and Game Department
o Wyoming Game and Fish Department
o Nevada Department of Wildlife Disease

Arizona National Poultry Improvement Plan Surveillance

California Department of Fish and Game Bighorn Sheep Surveillance

Nevada Department of Agriculture Diagnostic Services

Nevada Department of Wildlife: Bighorn Sheep Surveillance

Arizona Department of Game and Fish: Bighorn Sheep Surveillance
6
3. Personnel Changes (New or Lateral Hires):
Departures
Joe Beier, Histology
Dr. Kevin Lahmers, Pathology
Dr. James Stanton, Pathology
Claire Warren, Avian Health & Food Safety Laboratory
Jason Wetzel, Information Technology
New Faculty and Staff
Dr. Dharani Ajithdoss, Pathology
Nicole Dossey, Sample Receiving
Larry Hughes, Information Technology
Rebecca Kottke, Molecular Diagnostics
Dr. Josh Ramsay, Pathology
Andrew Vo, Aquaculture
4. Capital and Equipment Improvements
Capital Improvements
 WADDL –Puyallup (Avian Health and Food Safety Laboratory)
o Necropsy and Biowaste Renewal Project
o VOIP Phone System Upgrade
o Security System Upgrade
Equipment Improvements
 WADDL-Pullman: Replaced desktop computers
o Bacteriology: MALDI-TOF Biotyper CM System; 2 Zeiss
Microscopes
o Histology: Rotary Microtome
o Serology: Microplate Reader software; Ultra-low chest freezer
o Sample Receiving: Established new section to receive, record, and
distribute samples to the various WADDL sections.

WADDL-Puyallup (Avian Health and Food Safety Laboratory)
o Bio-Rad Gel Imager
o Egg Incubator
o Leased new copier/scanning machine
o Remodel room for new section
7
5. Discovery:
Scholarly Activity
 WADDL faculty and staff in FY2014 wrote 37 peer-reviewed publications
and 13 book chapters, and made 41 presentations at regional, state, or
national / international meetings (details in “Publications in WADDL by
Faculty and Staff, pages 27-35)

Diseases / Disease Agent Discoveries
o Very Virulent Infectious Bursal Disease Virus (IBDV) associated
high mortality in Washington chickens
o Equine Herpesvirus-7 like gamma herpesvirus associated
abortion in donkeys
o Sarcocystis chalchasi encephalitis in psittacine birds

Tests developed / implemented
o MALDI-TOF Biotyper 24 turnaround time on bacterial cultures
o Coxiella burnetii immunohistochemistry
o Infectious bursal disease virus PCR
o Salmonella spp. PCR
8
DEFINITIONS:
Accession:
specimen(s) of one animal species or miscellaneous substances
belonging to one owner, and submitted at one time
Aquatic Laboratory Exams:
each fish or tissue pool tested
Bacteriologic Examinations:
each tissue sample cultured or examined
Examination: individual laboratory test or slide examined
Fixed Tissue: biopsy or necropsy tissue submitted in a fixed state
FY: Fiscal year (July 1 – June 30)
Laboratory Test: each specimen examined by a procedure to detect, identify or
quantify one or more significant substances to establish the nature of a condition
or disease.
Pathology slides:
number of slides examined histologically
Pathology tissues: number of tissues examined histologically
Immunohistochemistry slides: number of slides examined
immunohistochemically
Laboratory Animal:
rabbits, mice, hamsters, monkeys, rats, guinea pigs, etc.
Molecular Diagnostics: each test performed
Necropsy:
each whole animal examined
Parasitology:
Serology:
Toxicology:
each test performed
each test performed
each analysis performed
Virus Isolation:
each specimen cultured
9
WADDL Personnel
Laboratory Section
Name
Position
McElwain, Terry
Baszler, Tim
Leathers, Charles
Hagen, Kathleen
Weber, Judy
Aukerman, Denise
Olson, Diana
Boe, Judy
Everett, Erica
Lukens, Pat
Alexander, Trevor
Thompson, Jim
Lecoq, Merri
McMenamin, Katie
Reno, Jo-Lynn
Rockefeller, Joe
Vo, Andrew
Professor, Executive Director
Professor, Director
Professor, Associate Director
Administrative Manager
Fiscal Specialist Supervisor
Fiscal Technician 3
Patient Services Supervisor
Medical Transcriptionist 2
Office Assistant 2
Quality Manager
Quality Assistant (0.5FTE)
Associate Professor, Section Head,
ACVP Diplomate
Laboratory Manager
Microbiologist 1
Microbiologist 2
Laboratory Technician 2
Microbiologist 2
Crespo, Rocio
Associate Professor and Branch Chief
Masse, Jon
Meyer, Lynn
Perry, Stu
Warren, Claire
Weber, Sylvia
Williams, Cheryl
Office Assistant 3
Microbiologist 1
Microbiologist 2
Microbiologist 3
Microbiologist 1
Diaz-Campos, Dubra
Assistant Professor, Section Head,
Teitzel, Charlene
Alexander, Trevor
Baisley, Jennifer
Maddox, Timberly
Mellick, Dena
Wooten, Jeannine
Microbiologist 3 / Supervisor
Microbiologist 2 (0.5FTE)
Microbiologist 1
Microbiologist 2
Microbiologist 1 (0.5FTE)
Evermann, James
Professor, Section Head
Evans, Lusha
Merritt, Catherine
Noble, Kathy
Shiflett, Jessica
Tanaka, Lorraine
Wang, Tao
Laboratory Manager
Microbiologist 1
Microbiologist 2
Microbiologist 1
Microbiologist 2
Scientific Assistant
Administration
Administration Support
Budget and Finance
Front Office
Quality System
Aquatic Health
Avian Health and Food
Safety Laboratory
(WADDL-Puyallup Branch
Laboratory)
Bacteriology / Central
Processing/Parasitology
ImmunoDiagnostics /
Virology
Snekvik, Kevin
10
Information Systems
Histology /
Reichardt, Niles
Hughes, Larry
Whiteman, Aaron
Head, Medical Informatics
Info Systems Project Leader
Info Systems Coordinator
Leathers, Charles
Professor, Section Head
Leonard, Cassie
Babcock, Jennifer
Bailey, Gisela
Beier, Joesph
Smith, Lon
Bradway, Dan
Kottke, Rebecca
Bjorklund, Nicole
Wolking, Rebecca
Wooten, Jeanne
Laboratory Manager
Histologic Technician 1
Histotechnologist
Histologic Technician 1
Histotechnologist
Professor, Section Head, ACVM
Diplomate
Laboratory Manager
Scientific Assistant
Microbiologist 1
Microbiologist 1
Microbiologist 1
Ajithdoss, Dharani
Baszler, Tim
Haldorson, Gary
Leathers, Charles
Nelson, Danielle
Potter, Kathleen
Ramsay, Josh
Snekvik, Kevin
Prahinski, Kip
Assistant Professor, ACVP Diplomate
Professor, ACVP Diplomate
Associate Professor, ACVP Diplomate
Professor, ACLAM Diplomate
Assistant Professor, ACVP Diplomate
Instructor
Nadine Timm
Patricia Yager
Nicole Dossey
Laboratory Manager
Talcott, Patricia
Professor, ACVT Diplomate
Besser, Tom
Necropsy / Pathology
Sample Receiving
Toxicology
Graduate Students
Microbiology
Huntsberry, Claire
LeCuyer, Tessa
Rogovskyy, Artem
Pathology
Balmer Bethany
Ochoa, Jennine
Fry, Lindsay
Pastenkos, Gabrielle
Macias, Melissa
Schmidt, Carrie
Magunda, Foregivemore
Williams, Laura
Munoz, Juan
11
Annual Total Accession (Case) Information
The 5-year trend from FY2010 - FY2014 in WADDL accessions (client cases processed)
was upward, increasing from 18,106 in 2010 to 19,046 in 2014 (+5.2%). In FY 2014
WADDL-Pullman received 82.1% of total accessions and WADDL-AHFSL received 17.9%
of total accessions. Compared to FY 2013, FY2014 total WADDL accessions increased
2.5% (+465 accessions), reflecting a 1.9% increase at Pullman (+290 accessions) and a
5.9% increase at AHFSL (+191 accessions).
Total WADDL Accessions FY2010 - FY2014
WADDL-Pullman and WADDL-Puyallup (AHFSL)
22,000
20,324
19,555
20,000
18,566
18,106
19,046
18,000
16,000
14,000
12,000
10,000
2010 (FY)
2012 (FY)
Accessions-Pullman
FY2010- FY2014
18,000
16,000
2011 (FY)
15,569
15,994 15,723
15,341
5,000
2013 (FY)
Accessions-Puyallup (AHFSL)
FY2010 - FY2014
4,330
3,832
15,630 4,000
3,000
14,000
2014 (FY)
3,225
3,416
2,537
2,000
12,000
1,000
0
10,000
2010 2011 2012 2013 2014
(FY) (FY) (FY) (FY) (FY)
2010 2011 2012 2013 2014
(FY) (FY) (FY) (FY) (FY)
12
WASHINGTON
214, ANIMAL DISEASE DIAGNOSTIC
LABORATORY
544 - ANNUAL REPORT FY2014
Annual Total Laboratory Examination (Test) Information
The 5-year trend from FY2010 - FY2014 in WADDL laboratory tests conducted decreased
from 342,415 to 232,083 (-32.2%). The dramatic decrease in test numbers was due to loss
of the USDA Bovine Brucellosis Slaughter Surveillance Program in Washington State in
FY2012 (federal budget reductions). In FY2013 WADDL-Pullman conducted 85.7% of
total laboratory tests and WADDL-Puyallup (AHFSL) conducted 14.3% of total laboratory
tests. Compared to FY 2013, FY2014 total WADDL laboratory tests decreased 4%
(-13,313 tests), reflecting a 6.7% decrease at Pullman (-14,267 tests) and a 3% increase
at WADDL-Puyallup (AHFSL) (+954 tests).
400,000
Total WADDL Laboratory Tests FY2010 - FY2014
WADDL-Pullman and WADDL-Puyallup (AHFSL)
365,589
350,000
341,914
300,000
248,886
250,000
241,865
240,630
2013 (FY)
2014 (FY)
200,000
2010 (FY)
2011 (FY)
2012 (FY)
Labortory Tests-Pullman
FY2010 - FY2014
204,692
208,115
214,544
213,097
205,000
Laboratory Tests-Puyallup (AHFSL)
FY2010 - FY2014
198,830
156,973
162,000
142,000
155,000
137,723
122,000
102,000
105,000
82,000
62,000
55,000
42,000
34,342
32,299
33,253
2012 (FY)
2013 (FY)
2014 (FY)
22,000
5,000
2010 (FY)
2011 (FY)
2012 (FY)
2013 (FY)
2,000
2014 (FY)
2010 (FY)
13
2011 (FY)
Total WADDL Accessions by State
State
Accession
Alaska
447
Alabama
12
Arkansas
11
Arizona
192
California
439
Colorado
104
Connecticut
30
Delaware
0
District of Columbia
3
Florida
78
Georgia
41
Hawaii
25
Idaho
1,849
Illinois
32
Indiana
48
Iowa
278
Kansas
8
Kentucky
26
Louisiana
21
Maine
96
Maryland
29
Massachusetts
59
Michigan
145
Minnesota
29
Mississippi
8
Missouri
37
Montana
604
%
2.35%
0.06%
1.01%
1.01%
2.30%
0.55%
0.16%
0.00%
0.02%
0.41%
0.22%
0.13%
9.71%
0.17%
0.25%
1.46%
0.04%
0.14%
0.11%
0.50%
0.15%
0.31%
0.76%
0.15%
0.04%
0.19%
3.17%
State
Accession
Nebraska
82
Nevada
193
New Hampshire
33
New Jersey
24
New Mexico
107
New York
148
North Carolina
34
North Dakota
7
Ohio
79
Oklahoma
9
Oregon
883
Pennsylvania
51
Rhode Island
5
South Carolina
7
South Dakota
27
Tennessee
52
Texas
80
Utah
115
Vermont
7
Virginia
30
Washington
12,238
West Virginia
20
Wisconsin
26
Wyoming
21
Other
117
%
0.43%
1.01%
0.17%
0.13%
0.56%
0.78%
0.18%
0.04%
0.41%
0.05%
4.64%
0.27%
0.03%
0.04%
0.14%
0.27%
0.42%
0.60%
0.04%
0.16%
64.25%
0.11%
0.14%
0.11%
0.61%
Total
100.00%
19,046
Total Washington Accessions by WA County
County
Adams
Asotin
Benton
Chelan
Clallam
Clark
Columbia
Cowlitz
Douglas
Ferry
Franklin
Garfield
Grant
Grays Harbor
Accessions
47
260
308
75
73
194
34
23
22
19
390
27
284
34
%
0.38%
2.12%
2.52%
0.61%
0.60%
1.59%
0.28%
0.19%
0.18%
0.16%
3.19%
0.22%
2.32%
0.28%
County
Island
Jefferson
King
Kitsap
Kittitas
Klickitat
Lewis
Lincoln
Mason
Okanogan
Pacific
Pend Oreille
Pierce
San Juan
Accessions
47
28
1074
141
138
146
199
175
29
187
25
17
602
29
Whitman County includes WSU Veterinary Teaching Hospital
14
%
0.38%
0.23%
8.78%
1.15%
1.13%
1.19%
1.63%
1.43%
0.24%
1.53%
0.20%
0.14%
4.92%
0.24%
County
Skagit
Skamania
Snohomish
Spokane
Stevens
Thurston
Wahkiakum
Walla Walla
Whatcom
Whitman
Yakima
Not reported
TOTAL
Accessions
530
5
733
763
136
1477
26
394
617
1509
378
0
12,238
%
4.33%
0.04%
5.99%
6.23%
1.11%
12.07%
0.21%
3.22%
5.04%
12.33%
3.09%
0.00%
100%
FY 2014 WADDL Budget and Budget Sources
In FY 2014, the total WADDL budget from all budget sources (State, Revenue,
Grants and Contracts, and other) was $4,973,090. The majority of the WADDL
Budget (63%) was provided by non-State sources and includes revenue generated
from general fees for service (45%), and Grants and Contracts (18%) for state and
federal surveillance testing programs.
WADDL receives no state funding for operations. All state funding is expended for
faculty and staff salaries; state funding covers approximately 50% of total
personnel expenses.
Every state dollar invested in WADDL results in approximately $3 output from
WADDL through revenue generation or extramural grants and contracts (300%
return on investment).
FY 2014 WADDL Funding Sources
Total = $4,973,090
0%
18%
36%
State
Self-Sustaining
Grants
Other
46%
15
5 Year Trend for WADDL Budget and Budget Sources (FY2010 - FY2014)
The 5-year trend in total WADDL budget from FY2010 – FY2014 in total budget was
increased $296,316 (+6.6%), primarily from an increase in fee-for-service revenue of
$268,766 (+12.2%).
Compared to FY2013, FY2014 total fee-for-service revenue increased $72,652 (+3.3%).
Compared to FY2013, FY2014 total state support increased $75,426 (4.5%) due to “across
the board” professional staff and faculty salary increases granted and funded by the state.
On a percentage of total budget basis, the 5-year budget trend is relatively flat for state
support (blue bars), increasing for self-sustaining revenue (red bars), and relatively flat for
direct grants and contracts (light green bars).
Direct State Appropriations
Fee-for-Service Revenue
Grants and Contracts
Other
FY 2014
1,742,193
2,304,855
870,981 55,062
FY 2013
1,666,767
2,232,203
851,136 87,085
FY 2012
1,631,069
2,526,052
862,982 73,661
FY 2011
1,659,531
2,273,198
1,006,642 37,188
FY 2010
1,810,602
2,036,089
16
875,936 11,540
Total WADDL Tests by Laboratory Section (FY2010 - FY2014)
2010 (FY) 2011 (FY)
WADDL-Puyallup (Avian Health
Food Safety Laboratory)
2012 (FY) 2013 (FY) 2014 (FY)
137,723
156,973
34,342
32,299
33,253
Aquaculture
24,424
27,502
25,209
24,507
27,180
Bacteriology
11,691
10,042
11,346
10,775
8,058
BSE Lab (BSE and CWD)
Histopathology (Necropsy and
Surgical Biopsy)
8,607
5,617
8,727
7,639
6,658
5,164
5,307
4,760
4,423
4,609
2,753
1,762
1,386
1,774
1,800
11,415
15,557
12,520
14,379
13,093
982
1,022
1,004
977
1,249
3,570
2,910
2,288
2,548
2,208
Pathology Tissues
63,087
68,343
67,095
67,264
66,277
Serology
65,700
62,652
75,090
74,182
60,713
Toxicology
4,975
4,474
3,487
3,531
5,269
Virology (Electron Microscopy,
Isolation and ELISA)
2,324
2,927
1,632
1,098
1,716
342,415
365,088
248,886
245,396
232,083
Necropsy
Parasitiology
Total
Total WADDL Accessions by Species of Animal (FY2013 - FY2014)
Total WADDL-Pullman
Accessions by Species
Avian
Bovine
Camelid
Canine
Caprine
Equine
Feline
Fish
Ovine
Porcine
Wildlife/Other
Total
2014
(FY)
319
2538
236
5074
2521
1403
777
555
490
87
1307
15307
2013
(FY)
340
2680
288
4986
2419
1312
837
614
499
74
1292
15341
Total WADDL-AHFSL
Accessions by Species
Avian
Domestic Cow
Domestic Goat
Environmental
Food Product
Pig
Water
Other
Total
17
2014
(FY)
866
138
281
65
1740
9
257
38
3394
2013
(FY)
1079
149
7
274
1277
20
249
170
3225
Laboratory Examinations by Individual Branch / Section
WADDL-Puyallup (Avian Health and Food Safety Laboratory)
Bacteriology
Brucella Slaughter Testing
Food Safety
Histopathoolgy
Necropsy
Parasitology
Serology
Virology
Total
FY 2014
3,072
0
15,946
148
2,989
652
19
10,425
2
33,253
FY 2013
2,980
0
17,942
158
1,303
158
16
9,741
1
32,299
WADDL Pullman Laboratory
PATHOLOGY BY SPECIES
Avian
Bovine
Canine
Caprine
Equine
Feline
Lab Animal
Ovine
Porcine
Other
FY2014 TOTAL
NECROPSY
69
5.52%
168 13.45%
242 19.38%
60
4.80%
84
6.73%
113
9.05%
141
11.29%
49
3.92%
44
3.52%
279 22.34%
1249
100%
HISTOPATHOLOGY
FROM NECROPSY
44
5.19%
308
36.36%
95
11.22%
49
5.79%
25
2.95%
33
3.90%
51
6.02%
81
9.56%
47
5.55%
114
13.46%
847
100%
SURGICAL BIOPSY
13
0.35%
23
0.61%
3055
81.21%
11
0.29%
212
5.64%
362
9.62%
15
0.40%
1
0.03%
2
0.05%
68
1.81%
3762
100%
PATHOLOGY BY SPECIES
Avian
Bovine
Canine
Caprine
Equine
Feline
Lab Animal
Ovine
Porcine
Other
FY2013 TOTAL
NECROPSY
64
6.60%
132 13.50%
195 20.00%
43
4.40%
79
8.10%
132 13.50%
155 15.90%
87
8.90%
35
3.60%
55
5.60%
977
100%
HISTOPATHOLOGY
FROM NECROPSY
29
4.80%
78
13.00%
151
25.20%
26
4.30%
44
7.30%
100
16.70%
46
7.70%
66
11.00%
18
3.00%
41
6.80%
599
100%
SURGICAL BIOPSY
36
0.90%
61
1.60%
2,968
77.60%
18
0.50%
244
6.40%
398
10.40%
14
0.40%
13
0.30%
5
0.10%
67
1.80%
3,824
100%
18
WADDL – Pullman Laboratory
PARASITOLOGY
Baermann
Fecal Flotation
Liver Fluke Sedimentation
Parasite Identification
2014
202
1892
8
106
2,208
2013
260
2,066
58
164
2,548
2014
2013
7,671
3,969
2,109
9,268
6,892
3,626
1,980
8,190
Diagnostic Testing
Aquatic aerobic culture
Aquatic anaerobic culture
Aquatic susceptibility
Aquatic fungal culture
Aquatic viral isolation
265
1
0
1
17
614
2
0
0
29
Pathology
Aquatic necropsy
Aquatic histopathology
M. cerebralis histopathology
External parasite (Histo)
21
718
300
5
25
939
435
0
2.389
446
0
0
27,180
1,691
84
0
0
24,507
2014
290
177
467
2013
319
265
584
TOTAL PARASITOLOGY
AQUATIC HEALTH LABORATORY EXAMINATIONS
Health Inspection Testing
Aquatic bacterial screen
BKD
Piscirickettsia salmonis
Aquatic viral culture
Parasitology
M. cerebralis digest
C. shasta smear
External parasite screen
Amoebic Gill Disease
TOTAL AQUACULTURE
TERRESTRIAL ANIMAL VIROLOGY
Viral Identification-EM
Viral Isolation
TOTAL VIROLOGY*
*No Viral antigen ELISA, See ImmunoDiagnostics
19
MOLECULAR DIAGNOSTICS
Standard and Real time PCR
Anaplasma marginale
Aquaculture
Bacterial
Bluetongue
Bovine Virus Diarrhea Virus
Clostridium perfringens
Contagious ecthyma (Orf)
Contagious Bovine Pleuropneumonia
Classical Swine Fever Virus
E. coli virulence
Avian Influenza
2014
2013
249
1,953
46
62
4,708
71
20
231
34
40
256
593
1,580
13
65
6,899
85
0
0
16
73
475
Equine Herpesvirus 1
Exotic Newcastle' Disease Virus
Canine Influenza Virus
Enzootic Hemorrhagic Disease Virus
Lumpy Skin Disease Virus
Malignant Catarrhal Fever Virus
Miscellaneous PCR
Mycobact. avium spp. paratuberculosis
Mycoplasma spp.
Mycoplasma ovipneumoniae
Neospora caninum
Nested PCR
PCR from tissue
Rhodococcus equi
Toxoplasma gondii
Tritrichomonas foetus
West Nile Virus
DNA sequencing
Fungal ID by sequencing
Mycobacterium ID by sequencing
19
0
1
41
0
61
252
138
39
2,485
17
4
146
2
9
2,007
36
98
28
39
58
3
1
63
0
44
147
30
1,695
10
4
114
8
19
2,183
61
67
25
44
0
1
0
0
0
2
2
0
13,093
14,379
Select Agent PCR
African Swine Fever by PCR
Foot and mouth disease by PCR
Francisella tularemia
Rinderpest virus by PCR
TOTAL
20
BACTERIOLOGY
Cultures & Identifications
Aerobic
Anaerobic
Blood
Milk Bulk tank
Campylobacter spp.
Clostridium difficile
Clostridium perfringen
Cold enrichment culture
Enterococcus spp.
Fecal culture
Feed culture
Mycobacterium avium spp. paratuberculosis
Listeria spp.
Mastitis culture
Mycobacterium spp.
Mycology culture
Mycoplasma spp.
Salmonella spp.
Tritrichomonas spp.
Ureaplasma spp.
Susceptibilities
K/B Susceptibility
MIC Susceptibility
Other Bacteriology
Bacteriologic Stains
Clostridium difficile Toxin Test
Clostridium spp. F/A
Leptospirosis spp. F/A
Salmonella spp. Serotyping
TOTAL
21
2014
2013
3,840
724
94
12
96
68
148
0
15
777
1
25
34
68
18
243
466
90
3
14
4,457
931
100
2
166
42
171
0
9
702
7
31
25
184
51
222
1,210
235
22
13
0
574
0
714
1,136
67
8
179
65
8,765
1,182
39
12
186
62
10,775
IMMUNODIAGNOSTICS / SEROLOGY
Anaplasma marginale
Bluetongue Virus
Bovine Leukosis Virus
Bovine Parainfluenza-3
BRSV
Bovine Viral Diarrhea Virus
Brucella abortus
Brucella canis
Brucella ovis
Canine Adenovirus
Canine Coronavirus
Canine Distemper Virus
Canine Herpesvirus
Canine Parainfluenza
Canine Parvovirus
Small Ruminant Lentivirus (CAEV, OPP)
Caseous Lymphadenitis
Epizootic Hemorrhagic Disease
Equine Herpes Virus
Equine Infectious Anemia Virus
Equine Viral Arteritis
Feline Calicivirus
Feline Coronavirus/FIP
Feline Herpesvirus
Feline Immunodeficiency Virus
Feline Panleukopenia Virus
Infectious Bovine Rhinotracheitis Virus
Johne's Disease
Leptospira MAT
Malignant Catarrhal Fever Virus
Mycobacterium bovis gamma interferon
Mycoplasma ovipneumoniae
Neospora caninum
Ovine Progressive Pneumonia Virus
Porcine Parvovirus
Pseudorabies Virus
Toxoplasma gondii
Vesicular Stomatitis Virus
West Nile Virus
TOTAL SEROLOGY
22
2014
2013
3,302
854
2,141
638
606
1,130
8,504
47
290
30
1
851
15
8
360
12,264
7,812
550
57
1,325
865
34
163
37
51
105
767
8,422
6,897
0
87
1,261
902
0
9
63
235
0
30
60,713
5,333
2,602
3,186
553
546
1,190
13,198
0
115
59
47
349
22
21
316
16,544
8,670
561
52
731
346
20
51
60
5
46
655
7,811
7,557
104
81
1,540
1,000
611
10
0
125
38
27
74,182
IMMUNOHISTOCHEMISTRY
PROCEDURES
Infectious agents-Viruses
Bovine Coronavirus
Bovine Respiratory Syncytial Virus
Bovine Virus Diarrhea Virus
Canine Coronavirus
Canine parvovirus
Equine Herpesvirus-1
Infectious Bovine Rhinotracheitis Virus
Feline coronavirus (FIP)
Feline parvovirus (panleukopenia)
Parainfluenza Virus-3
Prions (Chronic Wasting Disease)
Prions (Sheep and Goat Scrapie)
2014
2013
2
2
13
3
7
11
1
20
8
2
1
33
1,528
0
6
24
1
14
6
0
5
18
7
0
95
1,371
3
4
0
0
0
1
24
7
0
0
41
0
24
3
11
1
7
5
4
2
1
43
20
53
1
17
9
10
1
5
16
8
0
1
55
19
1,800
1,774
Infectious agents-Bacteria & Protozoa
Coxiella
Leptospira
Listeria
Sarcocystis
Toxoplasma
Tumor Cell Markers
B-lymphocyte
Chromatogranin
Cytokeratin
Desmin
Factor VIII
GFAP
Lamda Light Chain
Melan A
Muscle Actin
Neurofilament
Synaptophysin
T-lymphocyte
Vimentin
TOTAL
23
IMMUNODIAGNOSTICS
/ ANTIGEN DETECTION
2014
2013
BSE
BRSV-Ag
BVD-Ag
Bovine Enteric Virus
Chlamydophila
CWD Ag ELISA
Feline Leukemia Virus
TOTAL
6,658
57
708
0
851
38
0
62
8,374
7512
121
573
0
349
43
127
12
8,737
University of Idaho Analytical Sciences Laboratory
TOXICOLOGY
Acetylcholinesterase - blood
Aflatoxin
Ammonia
Anticoagulant rodenticide screen -blood*
Anticoagulant rodenticide screen -tissue*
Arsenic - blood/serum
Ash %
Carbamate pesticide screen
Carbon & Nitrogen
Chloride - Nitric
Chlorinated Herbicides - Limited
Cyanide
Sample prep (filter, etc.)
Water metals - ICP - Dissolved
Water metals - ICP - Total
Water metals - ICPMS - Dissolved
2014
5
17
10
3
15
3
0
6
3
2
0
3
2
3
1
4
2013
2
11
6
0
10
2
1
1
0
0
0
0
18
1
0
0
Water metals - ICPMS - Total (Extended)
Glutathione peroxidase
Glycol - ethylene/other
Heavy metals screen -ICP - tissue
Heavy metals screen-ICPMS
Heavy metals -ICPMS -single analyte
Ion Screen
Ionophores
Lead - blood
Macro Element Screen
Macro/Micro Elements
Melamine
0
9
4
170
39
2
1
7
69
20
422
13
0
8
0
114
40
9
1
5
19
10
156
0
Mercury
Metaldehyde
Micro elements
Moisture percent
Molybdenum
Nitrate - feed
Nitrate - ocular fluid / serum
Nitrate/nitrite - water
Nitrite - feed
Nitrite - ocular/serum
Organochlorine pesticide screen
Organophosphorus/nitrogen screen
PCB Congener
pH
Phosphine gas [Zn-Mg-Al phosphide]
Plant / Mushroom identification
Selenium
Special request analysis
Strychnine
Toxicology organic [GCMS] screen
Trace elements - serum
Trace elements - ocular fluid
Trace elements, Na + K only
Trace elements - soil
Urea
Vitamin A - tissue
Vitamin E - serum
Vitamin E - tissue
Total
24
2014
27
2
0
10
0
7
19
4
0
0
10
20
5
4
1
2013
19
1
3
0
7
15
20
0
3
1
12
8
8
3
2
25
2,546
27
8
20
1,478
17
0
1
1
10
188
6
12
1,688
31
12
13
1,149
11
5
0
1
0
93
0
5269
3531
WADDL Quality System
External Audits Successfully Passed
 USDA Compliance Inspection for Permit 118210 in Pullman, September
2013
 National Poultry Improvement Plan Audit
o AHFSL, November 2013
o Pullman, December 2013
 USDA Approval Inspection to test for Contagious Equine Metritis (CEM), April
2013
Official WADDL Proficiency Testing and Inter-laboratory Comparisons
Test
Agency
Lab Section
WADDL-Pullman
USDA
USDA
AAVLD
Bacteriology
Avian Influenza PCR
Avian Paramyxovirus PCR
Aerobic Pathogens Inter-laboratory
Comparison
Aflatoxin in Milk
Bluetongue
Bovine Leukosis (BLV)
Bovine Spongiform Encephalopathy
Brucellosis Serologic Tests (SPT, BAPA,
STT, Rivanol and Card)
Bovine Tuberculosis Gamma Interferon
Brucella ovis ELISA
Chronic Wasting Disease (CWD) ELISA
Classical Swine Fever Virus
Equine Herpesvirus-1 Neuropathogenic
PCR Inter-laboratory Comparison
Equine Infectious Anemia (EIA)
European Union Aquatic Pathogen
Equine Viral Arteritis
5-Hydroflunixin in Milk
Johne’s Disease Milk ELISA
Johne’s Fecal PCR (individual)
Johne’s Fecal PCR (pooled)
Johne’s Disease Fecal Test (Solid media)
Johne’s Disease Serologic
Leptospira MAT
Melamine and Cyanuric Acid in Fish
Tissue
Newcastle’s Disease Virus (NDV) PCR
Pseudorabies gB
Pseudorabies gI
Salmonella V-Clasp (Bacteriology)
FDA
USDA
USDA
USDA
USDA
USDA
USDA
USDA
USDA
AAVLD /
USDA
USDA
EU
USDA
FDA
USDA
USDA
USDA
USDA
USDA
USDA
FDA
USDA
USDA
USDA
FDA
25
Bacteriology
Immunodiagnostics – Serology
Immunodiagnostics – TSE Lab
Immunodiagnostics – Serology and
AHFSL
Immunodiagnostics - Serology
Immunodiagnostics – TSE Lab
Aquatic Health
Bacteriology
Bacteriology
Toxicology
Molecular Diagnostics and AHFSL
Bacteriology
Test
Continued
Salmonella PCR Inter-laboratory
Comparison
Scrapie / Chronic Wasting Disease (CWD)
by Immunohistochemistry
Swine Influenza Virus (SIV) PCR
West Nile Virus (Equine IgM Capture
ELISA)
Agency
Lab Section
FDA
USDA
Pathology and Histology
USDA
USDA
WADDL – Avian Health and Food Safety Laboratory
Avain Influenza - AGID
USDA
Serology
Avian Influenza PCR
USDA
Avian Paramyxovirus PCR
USDA
Brucellosis Serologic Tests (SPT, BAPA,
USDA
Immunodiagnostics – Serology and
STT, Rivanol and Card)
AHFSL
Pseudorabies gB
USDA
Pseudorabies gI
USDA
Salmonella Group D
NPIP
AHFSL
Salmonella in Liquid Egg
USDA
Bacteriology, Molecular Diagnostics,
Serology
26
PUBLICATIONS / PRESENTATIONS BY WADDL FACULTY, RESIDENTS and STAFF
Presentations
Baszler T, Ostlund E, Crossley B, Balasuriya U, Bradway D, Erol E, Johnson D,
Rodman D, Sells S: Interlaboratory Comparison of Equine Herpesvirus type 1
Polymerase Chain Reaction Techniques Utilized in North American Diagnostic
Facilities. Amercian Association of Veterinary Laboratory Diagnosticians, San Diego,
CA October 18-22, 2013, Invited Plenary Session Speaker
Berlinski R, Hanley C, Garner M, Jafarey Y, Kinsella M, Bradway D, Meyerson R:
Meerkat (Suricata suricatta suricatta) Fatalities Associated with Intestinal
Trematodiasis (Prosthodendrium naviculum. American Association of Zoo
Veterinarians Annual Conference, Salt Lake City, Utah October 2013
Chiok K, Warren C, Crespo R, Shah DH: Evaluation of a multiplex real-time PCR for
the serotype-specific detection of Salmonella enteritidis. 113th General meeting of the
American Society of Microbiologists. Denver, Colorado. Poster: 853, 2013
Crespo R, Chiok K, Warren CA, Shah DH: Development of a multiplex real-time
PCR for the Serotype-specific Salmonella enteritidis. Abstracts in the Proceedings of
the 148th AVMA/AAAP Annual Convention, Chicago, Illinois, 2013
Crespo R: A Database for Equipment Maintenance and Reagent Tracking. Abstracts
in the Proceedings of the 149th AVMA/AAAP Annual Convention, Denver, Colorado,
2014
Crespo, R: Avian Influenza, Salmonella and Other Avian Zoonotic Diseases. Zoonotic
and Vector- Borne Diseases Workshop. Washington State Department of Health.
Olympia and Moses Lake, WA, 2014
Crespo, R: Common Health Conditions in Poultry. Country Living Expo & Cattlemen’s
Winterschool. Washington State University, Extension. Stanwood, WA, 2014
Crespo, R: Pasture Poultry management and challenges. Small Farms Program,
WSU CAHNR. WSU Extension and Research Center, Puyallup, WA, 2013
House M, Ward L, Snekvik K: Progress on the removal of the Elwha river damsSediment and more sediment. Western Fish Disease Workshop, Port Townsend, WA,
June 19, 2013
Justice-Allen A, Fry L, Bradway D, Snekvik K, Darveau D, Holt T: Bluetongue virus
10 and 11 infection in two desert bighorn sheep. Wildlife Disease Association
meeting, Knoxville, TN, July 2013
27
Leathers CW: WSU Feline Club, necropsy wet laboratory, Pullman, WA, October,
2013
Leathers CW: WSU Natural Resource Science, necropsy wet laboratory, Pullman,
WA, November, 2013
Leathers CW: University of Idaho Wildlife Ecology, necropsy wet laboratory, Moscow,
ID, December, 2013
Leathers CW: University of Idaho Wildlife Society, necropsy wet laboratory, Moscow,
ID, April, 2014
Leathers CW: Washington State 4-H Teen Conference, necropsy wet laboratory,
Pullman, WA, June, 2014
Leathers CW: WSU Animal Sciences, necropsy wet laboratory, Pullman, WA, April,
2014
Mitchel H, Snekvik K: The other KD: PKD- or is it? Eastern Fish Health Workshop,
Gettysburg, PA, Apr 30, 2013
MM Roiseco: Fatal intestinal inflammatory lesions from equine necropsy cases from
California: A case series study from 1990 to 2013. Annual meeting of the American
Association of Veterinary Laboratory Diagnosticians, San Diego California, October
2013
Munoz-Gutierrez JF, Schneider DA, Baszler TV, Stanton JB: An immortalized sheep
microglial cell line capable of accumulating natural and experimental prions. Prion 7
(Supplement): 44-45, 2013
Murray M, Sidor I, Garner M, Marchand M, Bradway D: Fungal dermatitis in two freeliving snakes associated with Chrysosporium ophiodiicola and Acremonium strictum.
Annual Conference of the Wildlife Disease Association, Knoxville, TN, July 2013
Noh SM: Emerging and re-emerging vector borne diseases; with a focus on bluetongue. Emerging and Exotic Diseases of Animals course, Washington State
University, College of Veterinary Medicine, 2014
Noh SM: Principles of vector-borne disease. APHIS Smith-Kilborne Transboundary
and Emerging Animal Disease course. Washington DC, 2014 (invited)
Potter KA, Besser TE, Cassirer EF. Mycoplasma ovipneumoniae infection in bighorn
sheep. Western Conference of Veterinary Diagnostic Pathologists, Saskatoon,
Saskatchewan, Oct 4-5, 2013
28
Potter, KA, Ochoa J. Fungal eustachiitis (guttural pouch mycosis). Western
Conference of Veterinary Diagnostic Pathol., Saskatoon, Saskatchewan, Oct, 2013
R. Subramaniam, S. Shanthalingam, J. Bavananthasivam, A. Kugadas, B. Raghavan,
S.A. Batra, J. Rodriguez, A. Tibary, D.D. Nelson, K.A. Potter, W.J. Foreyt, and S.
Srikumaran. Why are bighorn sheep more susceptible than domestic sheep to
Mannheimia haemolytica-caused pneumonia? 10th International Veterinary
Immunology Meeting, 2013
Rogovskyy AS and Bankhead T: Genetic heterogeneity is a requirement for host
superinfection by the Lyme disease spirochete. The 15th Annual College of Veterinary
Medicine Student Research Symposium, Washington State University, Pullman, WA,
October 29, 2013
Rogovskyy AS, Chen Z, Burk RD, and Bankhead T: Genomic comparison of the
novel papillomavirus isolated from the North American beaver (CcanPV1) to other
rodent papillomaviruses. Dr. William R. Wiley Research Exposition, Washington State
University, Pullman, WA, February 22, 2014
Rogovskyy AS, Chen Z, Burk RD, and Bankhead T: Genomic comparison of the
novel papillomavirus isolated from the North American beaver (CcanPV1) to other
rodent papillomaviruses. The 56th AAVLD/117th USAHA, San Diego, CA, October 1723, 2013
Rogovskyy AS, Jones CR, Owen JP, Scoles GA, and Bankhead T: Investigating the
importance of VlsE for the enzootic cycle of Borrelia burgdorferi. Gordon Research
Conference (GRC): Spirochetes, Biology of, January 19-24, 2014, Ventura Beach
Marriott, Ventura, CA, USA
Rogovskyy AS: An interstrain difference in the ability of Borrelia burgdorferi to
superinfect”. The 94th Annual Meeting of the Conference of Research Workers in
Animal Diseases (CRWAD), Chicago, Illinois, December 8-10, 2013
Schmidt CE, Miller CH, Bradway DS, and Haldorson GH: Listeria monocytogenes
hepatitis in a serval cat (Leptailurus serval): a case report. American
College of Veterinary Pathologists annual meeting, Montreal, Canada, December
2013
Snekvik K and Bradway D. Surveillance testing for infectious salmon anemia virus in
the Pacific Northwest: the laboratory perspective. Aquaculture America Conference,
Seattle, WA, February 2014
29
Stanton JB, Muñoz-Gutierrez JF, Evermann JE, Ridpath JF and Bradway DS:
Immortalized sheep microglia are permissive to a diverse range of ruminant viruses.
American College of Veterinary Pathologists annual meeting, Montreal, QC, Canada
December 2013
Talcott PA: Mushrooms: The Good, The Bad, and the Ugly, Tasty Treats Pets Should
Avoid, New and Used Topics in Toxicology, Medications: Common Drugs That
Companion Animals Ingest, Facts and Fiction Associated With Decontamination
Procedures, My Neighbor Poisoned My Dog Syndrome, Plants Your Loved One
Should Reconsider, Central Veterinary Conference, San Diego, October 31November 3, 2013
Talcott PA: Plants and Pesticides Problematic to Animals, Pesticide Management in
Cattle and Rangeland Operations, Ritzville, WA, May 14, 2013
Talcott PA: Plants Problematic to Animals – Identification and Control of Poisonous
Plants in Range and Pasture, 13th Annual Washington Country Noxious Weed Tour
and the Noxious & Invasive Weed Identification and Integrated Pest Management
Program, Weiser, ID, June 12-13, 2013
Talcott PA: Plants Problematic to Animals and People, Paradise Creek Regional
High School, Moscow, ID, May 24, 2013
Talcott PA: Poisonous Plants in Hayfields: Their Effects on Livestock, Weed Free
Hay Inspector Training, Poisonous Plants, Moscow, ID, March 25, 2013
Talcott PA: Tasty Treats Pets Should Avoid”, “Common and Uncommon Poisonings
That Can Occur in Roaming Companion Animals, My Neighbor Poisoned My Dog
Syndrome, and Facts and Fiction Regarding Decontamination Procedures: Is It Ever
Too Late?, 102nd Annual Conference, Washington State Veterinary Medical
Association, Yakima, WA, October 4-6, 2013
Talcott PA: Weeds/Pesticides Problematic To Animals, Washington State Weed
Association Weed Conference, Wenatchee, WA, November 6-8, 2013
Wood J, Johnson E, Allen K, Campbell G, Rezabek G, Bradway D, Pittman L, Little
S, and Panciera R: Merogonic Stages of Theileria cervi in Mule and White Tailed
Deer. 56th Annual Meeting of the American Association of Veterinary Laboratory
Diagnosticians (AAVLD), San Diego, CA, October 2013
30
Book Chapters
Besser TE, Schmidt C, Shah DH, & Shringi S: 'Pre-harvest' Food Safety for E. coli
O157 and other pathogenic STECs. In: Enterohemorrhagic Escherichia coli. American
Society for Microbiology Press, In Press, 2014
Crespo R and Shivaprasad HL: Miscellaneous diseases. In: Diseases of Poultry, 13th
edition. D. Swayne et al (eds). Iowa State Press, Ames, Iowa. Pages: 1233-1270,
2013
Crespo R and Shivaprasad HL: Velogenic Newcastle-slide study set. American
Association of Avian Pathologists, 2013
Evermann JF: Canine herpesvirus ecology and epidemiology. In Clinical Veterinary
Advisor: Dogs and Cats 3rd ed, Cote E ed. Elsevier, New York pp 510-513, 2014
Gwaltney-Brant, SM and Talcott PA: “Nonsteroidal antiinflammatories”. In: Small
Animal Toxicology, 3rd Edition, Ed. M.E. Peterson and P.A. Talcott, Saunders/Elsevier,
pp. 687-708, 2013
Murphy MF, Talcott PA: “Anticoagulant rodenticides”. In: Small Animal Toxicology,
3rd Edition, Ed. M.E. Peterson and P.A. Talcott, Saunders/Elsevier, pp. 435-445, 2013
Talcott PA: “Copper”. In: Small Animal Toxicology, 3rd Edition, Ed. M.E. Peterson
and P.A. Talcott, Saunders/Elsevier, pp. 517-521, 2013
Talcott PA: “Effective use of a diagnostic laboratory”. In: Small Animal Toxicology, 3rd
Edition, Ed. M.E. Peterson and P.A. Talcott, Saunders/Elsevier, pp. 125-132, 2013
Talcott PA: “Miscellaneous herbicides, fungicides, and nematocides”. In: Small
Animal Toxicology, 3rd Edition, Ed. M.E. Peterson and P.A. Talcott, Saunders/Elsevier,
pp. 401-408, 2013
Talcott PA: “Mycotoxins”. In: Small Animal Toxicology, 3rd Edition, Ed. M.E. Peterson
Talcott PA: “Strychnine”. In: Small Animal Toxicology, 3rd Edition, Ed. M.E. Peterson
Talcott PA: “Zinc”. In: Small Animal Toxicology, 3rd Edition, Ed. M.E. Peterson and
P.A. Talcott, Saunders/Elsevier, pp. 847-851, 2013
Wills TB and Haldorson GJ, Chapter 28 “The Adrenal Gland”, in Cowell and Tyler’s
Diagnostic Cytology and Hematology of the Dog and Cat, Fourth Edition, AC
Valenciano and RL Cowell Editors, Elsevier, 2014
31
Publications
Al-Adwani, S. R., R. Crespo, and D. H. Shah. Production and Evaluation of Chicken
Egg-Yolk-Derived Antibodies Against Campylobacter jejuni Colonization-Associated
Proteins. Foodborne Pathogen and Disease. 10: 624-631, 2013
Besser TE, Cassirer EF, Highland MA, Wolff P, Justice-Allen A, Mansfield K, Davis
MA, and Foreyt WJ: Bighorn sheep pneumonia: sorting out the cause of a
polymicrobial disease. Preventive Veterinary Medicine 108: 85-89, 2013
Burek-Huntington KA, Gill V, and Bradway DS: Locally Acquired Disseminated
Histoplasmosis in a Northern Sea Otter (Enhydra lutris kenyoni) in Alaska, USA.
Journal of Wildlife Diseases 50: 389-392, 2014
Chung C, Wilson C, Bandaranayaka-Mudiyanselage CB, Kang E, Adams DS,
Kappmeyer LS, Knowles DP, McElwain TF, Evermann JF, Ueti MW, Scoles GA, Lee
SS, McGuire TC: Improved diagnostic performance of a commercial Anaplasma
antibody competitive enzyme-linked immunosorbent assay using recombinant major
surface protein 5-glutathione S-transferase fusion protein as antigen. J. Vet. Diagn.
Invest. 26:61-71, 2014
Chung C, Wilson C, Evermann JF, et al. Validation of an improved competitive
ELISA for equine viral arteritis virus, J. Vet. Diag. Invest. 25: 727-735, 2013
Churgin SM, Garner MM, Swenson J, Bradway DS, and West G: Intestinal
Coccidioidomycosis in a Coachwhip Snake in a Red Coachwhip Snake (Maticophis
flagellum piceus). Journal of Zoo and Wildlife Medicine, 44:1094-1097. 2013
Crespo R, Franca M, Shivaprasad ML: Ulcerative enteritis-like disease associated
with Clostridium sordellii in quail.Avian Diseases. 57: 698-702, 2013
Crespo R, Hopkins S, Shah D: Outbreak of Listeria monocytogenes in an urban
poultry flock. Avian Diseases. BMC Veterinary Research. DOI:10.1186/1746-6148-9204, 2013
Crosby F. L., Wamsley H. L., Pate M. G., Lundgren A. M, Noh S. M., Munderloh U.
G., Barbet A. F.: Knockout of an outer membrane protein operon of Anaplasma
marginale by transposon mutagenesis. BMC Genomics. 15:278, 2014
Dassanayake RP, Evermann JF, et al. Role of Bibersteinia trehalosi, respiratory
syncytial virus, and parainfluenza-3 virus in Bighorn sheep pneumonia. Vet.
Microbiol.162: 166-172, 2013
32
Davis MA, Moore DA, Baker K, French N, Patnode M, Hensley J, Macdonald K, &
Besser TE: Risk factors for campylobacteriosis in two washington state counties with
high numbers of dairy farms. Journal of Clinical Microbiology 51: 3921-3927, 2013
Cassirer EF, Plowright RK, Manlove K, Potter KA, Cross PC, and Hudson PJ:.
Pneumonia in adult and juvenile bighorn sheep (Ovis canadensis): spatial and
temporal patterns within a metapopulation. Journal of Animal Ecology, doi:
10.1111/1365-2656.12031, 2013
Eberhart LJ, Ochoa J, Besser TE, and Call DR: Microcin MccPDI reduces the
prevalence of susceptible Escherichia coli in neonatal calves. Journal of Applied
Microbiology 117:340-346, 2014
Giannitti F, Rioseco MM, Garcia JP, Beingesser J, Woods LW, Puschner B, Uzal FA:
Diagnostic exercise: hemolysis and sudden death in lambs. Veterinary Pathology
51:624-627, 2014
Hove PR, Haldorson GJ, Magunda F, Bankhead T., Presence of Arp specifically
contributes to joint tissue edema associated with early-onset Lyme arthritis. Infect
Immun. 82: 43-51, 2014
Jaros P, Cookson A, Campbell DM, Besser TE, Shringi S, Mackereth GF, Lim E,
Lopez, L, Dufour M, Marshall JC, Baker MG, Hathaway S, Prattley DJ, & French N: A
prospective case-control and molecular epidemiological study of human cases of
Shiga toxin-producing Escherichia coli in New Zealand. BMC Infectious Diseases
(Online). Sep 30;13:450. doi: 10.1186/1471-2334-13-450, 2013
Jung W, Bono JL, Clawson M, Leopold S, Shringi S, & Besser TE: Lineage and
genogroup-defining single nucleotide polymorphisms of Escherichia coli O157:H7.
Applied and Environmental Microbiology 79: 7036, 2013
Mellor GE, Besser TE, Davis MA, Beavis B, Jung W, Smith HV, Jennison AV, Doyle
CJ, Chandry PS, Gobius KS, & Fegan N: Multilocus genotype analysis of E. coli
O157 from Australia and the USA provides evidence of geographic divergence.
Applied and Environmental Microbiology 79: 5050-5058, 2013
Nelson DD, Haldorson GJ, Stanton JB, Noh SM, Bradway DS, Mansfield KG,
Baszler TV: Tularemia without lesions in grey tree squirrels: A diagnostic challenge.
Journal of Veterinary Diagnostic Investigation 26:312-315, 2014, [Epub 2014 Feb 20]
33
Oidtmann B, Lapatra S, Verner-Jeffreys D, Pond M, Peeler E, Noguera P, Bruno D,
St-Hilaire S, Schubiger C, Snekvik K, Crumlish M, Green D, Metselaar M, Rodger H,
Schmidt-Posthaus H, Galeotti M, Feist S. Differential characterization of emerging
skin diseases of rainbow trout – a standardized approach to capturing disease
characteristics and development of case definitions. J Fish Dis. 2013 Mar 1. doi:
10.1111/jfd.12086.
Plowrigh, R, Manlove K, Cassirer EF, Cross PC, Besser TE, and Hudson P: Use of
exposure history to identify patterns of immunity to pneumonia in bighorn sheep (Ovis
canadensis). PLoS One. 8(4):e61919, 2013
Rimoldi G, Speer B, Wellehan JF Jr, Bradway DS, Wright L, Reavill D, Barr
BC,Childress A, Shivaprasad HL, Chin RP: An outbreak of Sarcocystis chalchasi
encephalitis in multiple psittacine species within an enclosed zoological aviary. J Vet
Diagn Invest. 25: 775-781, 2013
Rogovskyy AS and Bankhead T: Bacterial heterogeneity is a requirement for host
superinfection by the Lyme disease spirochete. Infection and Immunity, ePub ahead
of print August 11, IAI.01817-14, 2014
Rogovskyy AS and Bankhead T: Variable VlsE is critical for host reinfection by the
Lyme disease spirochete. PLOS ONE, 8(4):e61226, doi:
10.1371/journal.pone.0061226, Print 2013
Rogovskyy AS, Chen Z, Burk RD, and Bankhead T: Characterization and analysis of
the North American beaver (Castor canadensis) papillomavirus genome. Vet.
Microbiol., 168:214-220, 2014
Sondgeroth K, Schlee S, Davis M, Evermann J, McElwain T, Baszler T:
Seroprevalence of Coxiella burnetii in Washington state domestic goats. Vector Borne
and Zoonotic Diseases 13:779-783, 2013 [Epub 2013 Oct 9]
Sondgeroth KS, McElwain TF, Allen AJ, Chen AV, Lau AOT: Loss of neurovirulence
is associated with reduction of cerebral capillary sequestration during acute Babesia
bovis infection. Parasites & Vectors Jun 18;6(1):181. doi: 10.1186/1756-3305-6-181,
2013
Stone D, Davis MA, Baker K, Besser TE, Roopnarine R, & Sharma R: MLST
genotypes and antibiotic resistance of Campylobacter spp. isolated from poultry in
Grenada. Biomedical Research International. 2013:794643; Epub Feb 24, 2013
Subramaniam R, Shanthalingam S, Bavananthasivam J, Kugadas A, Raghavan B,
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Bighorn sheep x domestic sheep hybrids survive Mannheimia haemolytica challenge
in the absence of vaccination. Veterinary Microbiology. 170: 278-283, 2014
34
Suthar N, Roy S, Call DR, Besser TE, and Davis MA: An individual-based model of
transmission of resistant bacteria in a veterinary teaching hospital. PLoS One
9(6):e98589, 2014
Volkova VV, Lu Z, Besser TE, and Grohn Y: Modeling Infection Dynamics of
Bacteriophages in Enteric Escherichia coli: Estimating the Contribution of
Transduction to Antimicrobial Gene Spread. Applied and Environmental Microbiology
80:4350-4362, 2014
Wattegedera S, Pearce J, Wheelhouse N, Baszler T, Cocheva M, Ballingall K,
McInnes C, Entrican G: Mechanisms of immunity and maternal tolerance in the ovine
placenta. Immunology 140:28, Supplement 1, 2013
Wheelhouse N, Coyle C, Barlow P, Mitchell S. Greub G, Baszler T, Rae M,
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July 10]
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(Oncorhynchus mykiss) Possess a Large Core Intestinal Microbiota That Is Resistant
to Variation in Diet and Rearing Density. Appl Environ Microbiol. 2013
Aug;79(16):4974-84. doi: 10.1128/AEM.00924-13. Epub Jun 14, 2013
Wood J, Johnson EM, Allen KE, Campbell GA, Rezabek G, Bradway DS, Pittman
LL,Little SE, Panciera RJ. Merogonic stages of Theileria cervi in mule deer
(Odocoileus hemionus). J. Vet. Diagn. Invest. 25:662-665, 2013
Ziegler J, Parish S, Snekvik K, Barrington G. Traumatic gastroperitonitis (hardware
disease) in an alpaca (Vicugna pacos). J Zoo Wildl. Med. 44:163-166, 2013
Ziegler JC, Lahmers KK, Barrington GM, Parish SM, Kilzer K, Baker K, & Besser TE:
Safety and immunogenicity of a Mycoplasma ovipneumoniae bacterin for domestic
sheep (Ovis aries). PLoS One ((4):e95698, 2014
35

WASHINGTON ANIMAL DISEASE DIAGNOSTIC LABORATORY

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