Diagnostic Laboratory Updates — Pennsylvania Animal Diagnostic

Diagnostic Laboratory
Updates
Pennsylvania Animal Diagnostic Laboratory System
Deep Tewari
-PADLS how it works
-Disease issues
-What is new
-Future
How does PADLS work
3 separate laboratories with 1 mission
Pennsylvania State University, ADL
University of Pennsylvania, NBC
PA Department of Agriculture, Harrisburg
1998 – creation of Animal Health and
Diagnostic Commission
PADLS memberships
-NAHLN
-FERN
-LIRN
Select Agent Membership
Registered BSL3 space
Additional BSL3 space operated as
BSL2 space
A member of NARMS- FDA and Human
The PADLS Concept
All 3 labs have a set of core disciplines:
Pathology
Bacteriology
Molecular testing
Each lab has a specialty:
PDA serology and regulatory focus
PSU mastitis and traditional virology
UP toxicology
The PADLS Concept (cont’d)
Single Quality system
Specimen transfer by courier
Single fee schedule
Single, lab information management system
Frequent and regular meetings of lab management
Accreditation
Tied to extension group for providing investigative
Who do we serve? Poultry, Cattle, Equine, Cervids,
Small ruminants,Aquaculture, Pet
and exotics
Core and Special Disciplines
Tests
20013-14
PADLS
Total
Serology
Pathology
571,174
451,174
50,144
Microbiol 77,648
Tox
3,022
PADLS Labs
NBC
PSU
PVL
PADLS contribution
Work on major ongoing disease programs
2013-14
Avian influenza
Brucellosis
Equine infectious anemia
Johne’s disease
Salmonella
Bovine Viral Diarrhea
Rabies
Mycoplasma (Avian) serology
181,427
41,269
14,004
25,908
19,563
17,117
1,638
90,363
PADLS
PEQAP (PA Egg Quality Assurance Program)
Began in 1994
Voluntary HACCP-type program
PDA, PA Health Dept., Penn Ag
Approx. 200,000 eggs tested per year
Tests added recently
TB testing -Gamma test for cattle
Parasitology testing equine/alpaca
Canine/Equine Influenza
Q fever
Johne’s in sheep and goats
SECD
FPA
IMPORTANT DISEASE ISSUES
Brucellosis
RAP
FPA
Milk ring
SAT
CF
Chasing false positives can be costly!
IMPORTANT DISEASE ISSUES
Bovine viral diarrhea
Multiple test options
Sale: PI animal check
ELISA- most popular
PCR
VI/Microplate test
Herd screen-Ear notch pooling most
Popular but vaccination and
collection issues can pose problems
IMPORTANT DISEASE ISSUES
Johne’s
A dairy herd in NE US
>30% animal tested come up seropositive
Negative on PCR and no clinicals
What are options?
Management issues
Other possibilities-birds??
Rotational testing Serology, Culture/PCR
IMPORTANT DISEASE ISSUES
Salmonellosis Cattle
Salmonella Newport
Salmonella Cerro
Salmonella Dublin
Biosecurity?
IMPORTANT DISEASE ISSUES
Avian Influenza
HPAI
LPAI
LBM, NPIP, USDA WS
All HPAI Detections in All Birds, by Type, As Of April 23, 2015 PM (as reported on www.aphis.usda.gov)
Backyard
Commercial
Wild Birds
CWD depopulations
BSL3 necropsy space
-16 deer
-13 deer
Tissue collection
Incineration
Protein Misfolding Cyclic Amplification
Invitro antemortem test
-amplification
-normal
+ abnormal prion
-detectable level of Prion
Disease issues: Your opinions
Strangles: Vaccine vs Non vaccine strains
Coggins: Reporting to SAHO in other
States for negatives
Rabies: Euthanasia issues
What’s new in Labs?
NAHLN New proposed structure
What is going to change?
Tier 1
= Core laboratory/Large BSL3
Tier 2
= Member laboratory
Tier 3
= Surveillance/Affiliate/Speciality
-New technology in labs
Multiplexing
Metagenomics/genome sequencing
MALDI
-New Biosafety initiatives-BMBL new version
Select agent rule easing (Brucella)
Multiplexing
Multiple agents with 1 test/1 sample
Lyme disease test (Cornell)
Salmonella/Campy/Clostd/E coli
(13 different targets)
Serological tests
LUMINEX based Technology.
Metagenomics
Viral-More common
Bacterial
Construction of Library
Shotgun sequencing
Looking at different fragment
Honey bee collapse disorder
MALDI-Bacterial identification
Bacterial: Is a norm in most mid –big
size D labs!
Based on signature peptide profile comparing
it to database.
PADLS –the Future
More collaborations/complete service
Reliable results
Qualified, experienced staff
Quality assurance, to OIE standards
Rapid results
Competent staff/scalability
State of the art instruments
Adequate staffing
PADLS –the Future (cont’d)
Good communication with clients
Connect LIMS to the web
Clients can input data
Clients have access to results
Enhanced capabilities
Emerging diseases
Foreign animal disease
Bioterrorism
PADLS services for supporting PA Agriculture
Acknowledgements: PVL, PADLS and Bureau staff