Brochure INVINCITY - In-Cosmetics North America

ALGUES & MER
OUESSANT ISLAND: OUTSTANDING PLACE
This fantastic environment is protected by many institutions,
like UNESCO (biosphere reserve).
A&M: THE FUCOIDANS EXPERT
INVINCITY® is extracted from Ascophyllum nodosum, harvested on the island before being
processed in our factory.
Fully in the sustainable way, Algues & Mer uses a hand cutting harvest method.
This provides regeneration stimulation and a quick new growth.
All our seaweeds on Ouessant Island are certified organic.
INVINCITY® is concentrated in High Molecular Weight (HMW) Fucoidans, over 100 kDa.
Naturally present in brown seaweeds only, Fucoidans are sulphated polysaccharides.
Main monomer is Fucose.
Fucoidans are well-known for many different benefits in cosmetics and medicine.
Each year, scientists find new properties out of these molecules, detailed in hundreds of
scientific publications.
Algues & Mer has a unique technical know-how to produce Fucoidans of different molecular weights.
Like hyaluronic acid, effects of Fucoidans on the skin depend on their molecular weights.
A&M Low Molecular Weight Fucoidans are reserved, with worldwide exclusivity, for a major brand.
The secret weapon against POLLUAGING®
TECHNICAL
INFORMATION
Appearance: Clear to cloudy liquid
INCI: Ascophyllum nodosum Extract, Sodium Benzoate,
Potassium Sorbate
Origin: Certified organic brown seaweeds of Ouessant Island
Dry matter: 6 to 7%
Solubility: Water soluble
Recommended dose: 3%
Processing:
• Emulsion: add INVINCITY® below 50°C, during the cooling
process.
• Lotion, serum: add INVINCITY® at any time,
in a pH range of 3.5 to 8.
APPLICATIONS
• Anti-pollution products
• Global anti-aging care
• Soothing care, anti-redness for sensitive skin
• Whitening, brightening and lightening products
• Concealer (CC cream...)
• After sun products
• Your next innovation!
Enjoy
Ouessant
unpolluted
fresh air!
Contacts & distributors list: www.algues-et-mer.com
©Algues & Mer - Argu Invincity - Mars 2016 ; Crédits photos: 123RF/ Gromovataya - Songquan Deng – Guniita ; Photo algues & Ouessant ©Algues & Mer
Factory and laboratory are located on Ouessant Island, 20 km off Brittany coast,
at the western tip of France, far from any source of industrial or agricultural pollution.
PROTECTS
Anti-pollution •
(AhR regulation)
Barrier reinforcement •
REPAIRS
• Anti-dark spots
• Anti-redness
• Higher collagen
density
FIGHTING POLLUAGING®
INVINCITY® EFFICACY
PROTECTS
1
❶
REPAIRS
2
❷
3
❸
GLOBAL BRIGHTNESS
1
ANTI-POLLUTION ❶
AhR (Aryl hydrocarbon Receptor) are receptors to polyaromatic hydrocarbons and some heavy metals. They are found
in keratinocytes, melanocytes, fibroblasts & Langerhans cells.
When activated by a pollutant, they trigger the expression of genes controlling reactions like oxidative stress,
inflammation, melanogenesis…
AhR expression was visualized with an antibody and quantified after exposure to pollutants:
INVINCITY® 3% decreases AhR expression by 73% compared to placebo (ex-vivo).
3
ANTI-DARK SPOTS ❸
4
❹
Inhibion of Tyrosinase acvity: up to 76%
The tyrosinase is a key enzyme of the melanogenesis. It contributes
to the melanin production by catalyzing the first chemical
reactions of the synthesis pathway, leading to dark spots.
INVINCITY® shows a significant inhibition of tyrosinase
activity with a dose‐dependent effect (in-tubo).
Melanin content variaon (%)
Melanin content: -8%
Untreated skin
AhR appear in pink
Skin + Polluting agents
(heavy metals + hydrocarbons)
Skin + Polluting agents
+ INVINCITY® 3%
REPAIRS
 Inflammation
 Melanogenesis
 Oxidative stress
Number of mature
cornified envelopes


Skin barrier function
 Water loss
Melanin
synthesis

Anti-dark spots
ANTI-POLLUTION
BARRIER
REINFORCEMENT
Cancels VEGF
peaks
Inhibits tyrosinase

Vasodilatation
 Capillary
permeability


Collagen
density
TRANSEPIDERMAL WATER LOSS (TEWL)
TEWL measurement is an indicator of the barrier dysfunction.
After 56 days, INVINCITY® 3% decreases the TEWL by 13% versus placebo (in-vivo).
Skin mechanical
resistance
GLOBAL BRIGHTNESS
-2%
-4%
8%*
FIRMNESS
INVINCITY®
INVINCITY®
20
10
0
INVINCITY®
0.5%
1%
INVINCITY®
INVINCITY®
2%
5%
6%
-5%
-6%
-7%
■ Placebo ■ INVINCITY® 3%
Hemoglobin content was measured with the Siascope® on a redness area.
After 56 days, INVINCITY® 3% decreases hemoglobin content by 9%
compared to placebo (in-vivo).
Hemoglobin content: up to -9%
850
800
Immature
corneocytes
750
700
650
+39%
42%
41%
41%
D28
5%
-6%
-8%
-10%
-12%
500
CTR
CTR+
H202
+ H2O2
INVINCITY®
0.5%
+ H2O2
INVINCITY®
2%
+6%
D56
4%
+5%
76%
+2%
43%
9%**
■ Placebo
4%
■ INVINCITY® 3%
+ H2O2
INVINCITY®
5%
5
FIRMNESS ❺
13%
In the dermis, collagen brings structural support to the
skin, and therefore its firmness.
The analysis of the general morphology shows that
INVINCITY® 3% improves the epidermal morphology
and increases collagen density in the papillary
dermis (ex-vivo).
Altered zone (non-viable)
3 to 4 cell layers
Altered zone (non-viable)
2 to 3 cell layers
Non altered zone (viable)
1 to 2 cell layers
Non altered zone (viable)
3 to 4 cell layers
Dense collagen network
Mildly dense collagen network
Skin + Polluting agents
(heavy metals + hydrocarbons)
EX-VIVO TEST CONDITIONS IN-VIVO TEST CONDITIONS
Human skin explants treated 5 days with INVINCITY® 3% versus placebo.
On day 5: a mix of pollutants (heavy metals + hydrocarbons) was added for 24h.
Parameters analyzed on day 6:
• General morphology of the skin (Masson’s trichrome stain)
• Expression of AhR (immunolabelling)
Double-blind study versus placebo on 15 urban women
(over 35 years old – average: 53 years old) with redness and dark spots on the face.
Twice-daily application on half face for 56 days:
• 1st side of the face: placebo cream
• 2nd side of the face: cream with 3% INVINCITY®
(1) Biological effect of various sulfated polysaccharides extracted from algae on skin barrier function through their impact on corneocyte maturation, Potter et al. ; 2011
D28
D56
-2%
-4%
D56
INVINCITY® 3%
7%
6%
5%
4%
3%
2%
1%
0%
-1%
-2%
-3%
-4%
-5%
550
TEWL variaon vs placebo
up to -13%
0%
D0
600
-14%
Anti-redness
30
Melanin content was measured with the Siascope® on a darkspot area.
After 28 days, INVINCITY® 3% decreases melanin content
by 8% compared to placebo (in-vivo).
0%
-1%
-3%
55%
43%
VEGF: Back to basic level aer oxidave stress
TEWL variaon (%)
AhR expression
40
63%
53%
50
900
Mature
corneocytes

60
0.1%
+2%
1%
76%
70
back to the baseline level.
VEGF ng/L
PROTECTS
80
2%
VEGF is a mediator of the vascular inflammation: it boosts the
vasodilatation and capillary permeability which lead to redness.
The VEGF modulation was evaluated on human keratinocytes
undergoing inflammation by oxidative stress (in-vitro).
From 0.5% of INVINCITY®, the VEGF concentration comes
CORNEOCYTES MATURATION
The first role of skin is to protect the body from external attacks.
A deficit of mature corneocytes leads to a dysfunctional barrier.
The impact of Fucoidans from Ascophyllum nodosum extract on the Cornified Envelope (CE)
maturation process was evaluated on reconstructed epidermal skin (ex-vivo; EpiskinTM model).
Native Fucoidans 5% increase the number of mature CE by 225%.(1)
90
4
ANTI-REDNESS ❹
2
BARRIER REINFORCEMENT ❷
The secret weapon
against POLLUAGING
3%
100
Hemoglobin content variaon (%)
Algues & Mer (A&M) innovates with an incredible active
ingredient: INVINCITY®.
It protects the skin from pollution from the inside,
and fights its damages, to prevent POLLUAGING®.
5
❺
% Inhibion enzyme acvity
Half of the world’s population lives in cities. Pollution is now
a major concern for consumers.
What is the impact on Earth? On my health?
And on my skin!?
Exposed to urban pollution, the skin becomes more sensitive.
It loses its brightness and ages prematurely.
This is « POLLUAGING® ».
Skin + Polluting agents
+ INVINCITY® 3%
Parameters analyzed:
• Redness and dark-spot evaluation with Siascope®
• TransEpidermal Water Loss (TEWL) measurement with Tewameter®
Results for the 10 best volunteers.
*Significant p<0.05; ** Significant p<0.01 ‐ Student’s t‐test
ALGUES & MER
OUESSANT ISLAND: OUTSTANDING PLACE
This fantastic environment is protected by many institutions,
like UNESCO (biosphere reserve).
A&M: THE FUCOIDANS EXPERT
INVINCITY® is extracted from Ascophyllum nodosum, harvested on the island before being
processed in our factory.
Fully in the sustainable way, Algues & Mer uses a hand cutting harvest method.
This provides regeneration stimulation and a quick new growth.
All our seaweeds on Ouessant Island are certified organic.
INVINCITY® is concentrated in High Molecular Weight (HMW) Fucoidans, over 100 kDa.
Naturally present in brown seaweeds only, Fucoidans are sulphated polysaccharides.
Main monomer is Fucose.
Fucoidans are well-known for many different benefits in cosmetics and medicine.
Each year, scientists find new properties out of these molecules, detailed in hundreds of
scientific publications.
Algues & Mer has a unique technical know-how to produce Fucoidans of different molecular weights.
Like hyaluronic acid, effects of Fucoidans on the skin depend on their molecular weights.
A&M Low Molecular Weight Fucoidans are reserved, with worldwide exclusivity, for a major brand.
The secret weapon against POLLUAGING®
TECHNICAL
INFORMATION
Appearance: Clear to cloudy liquid
INCI: Ascophyllum nodosum Extract, Sodium Benzoate,
Potassium Sorbate
Origin: Certified organic brown seaweeds of Ouessant Island
Dry matter: 6 to 7%
Solubility: Water soluble
Recommended dose: 3%
Processing:
• Emulsion: add INVINCITY® below 50°C, during the cooling
process.
• Lotion, serum: add INVINCITY® at any time,
in a pH range of 3.5 to 8.
APPLICATIONS
• Anti-pollution products
• Global anti-aging care
• Soothing care, anti-redness for sensitive skin
• Whitening, brightening and lightening products
• Concealer (CC cream...)
• After sun products
• Your next innovation!
Enjoy
Ouessant
unpolluted
fresh air!
Contacts & distributors list: www.algues-et-mer.com
©Algues & Mer - Argu Invincity - Mars 2016 ; Crédits photos: 123RF/ Gromovataya - Songquan Deng – Guniita ; Photo algues & Ouessant ©Algues & Mer
Factory and laboratory are located on Ouessant Island, 20 km off Brittany coast,
at the western tip of France, far from any source of industrial or agricultural pollution.
PROTECTS
Anti-pollution •
(AhR regulation)
Barrier reinforcement •
REPAIRS
• Anti-dark spots
• Anti-redness
• Higher collagen
density
FIGHTING POLLUAGING®
INVINCITY® EFFICACY
PROTECTS
1
❶
REPAIRS
2
❷
3
❸
GLOBAL BRIGHTNESS
1
ANTI-POLLUTION ❶
AhR (Aryl hydrocarbon Receptor) are receptors to polyaromatic hydrocarbons and some heavy metals. They are found
in keratinocytes, melanocytes, fibroblasts & Langerhans cells.
When activated by a pollutant, they trigger the expression of genes controlling reactions like oxidative stress,
inflammation, melanogenesis…
AhR expression was visualized with an antibody and quantified after exposure to pollutants:
INVINCITY® 3% decreases AhR expression by 73% compared to placebo (ex-vivo).
3
ANTI-DARK SPOTS ❸
4
❹
Inhibion of Tyrosinase acvity: up to 76%
The tyrosinase is a key enzyme of the melanogenesis. It contributes
to the melanin production by catalyzing the first chemical
reactions of the synthesis pathway, leading to dark spots.
INVINCITY® shows a significant inhibition of tyrosinase
activity with a dose‐dependent effect (in-tubo).
Melanin content variaon (%)
Melanin content: -8%
Untreated skin
AhR appear in pink
Skin + Polluting agents
(heavy metals + hydrocarbons)
Skin + Polluting agents
+ INVINCITY® 3%
REPAIRS
 Inflammation
 Melanogenesis
 Oxidative stress
Number of mature
cornified envelopes


Skin barrier function
 Water loss
Melanin
synthesis

Anti-dark spots
ANTI-POLLUTION
BARRIER
REINFORCEMENT
Cancels VEGF
peaks
Inhibits tyrosinase

Vasodilatation
 Capillary
permeability


Collagen
density
TRANSEPIDERMAL WATER LOSS (TEWL)
TEWL measurement is an indicator of the barrier dysfunction.
After 56 days, INVINCITY® 3% decreases the TEWL by 13% versus placebo (in-vivo).
Skin mechanical
resistance
GLOBAL BRIGHTNESS
-2%
-4%
8%*
FIRMNESS
INVINCITY®
INVINCITY®
20
10
0
INVINCITY®
0.5%
1%
INVINCITY®
INVINCITY®
2%
5%
6%
-5%
-6%
-7%
■ Placebo ■ INVINCITY® 3%
Hemoglobin content was measured with the Siascope® on a redness area.
After 56 days, INVINCITY® 3% decreases hemoglobin content by 9%
compared to placebo (in-vivo).
Hemoglobin content: up to -9%
850
800
Immature
corneocytes
750
700
650
+39%
42%
41%
41%
D28
5%
-6%
-8%
-10%
-12%
500
CTR
CTR+
H202
+ H2O2
INVINCITY®
0.5%
+ H2O2
INVINCITY®
2%
+6%
D56
4%
+5%
76%
+2%
43%
9%**
■ Placebo
4%
■ INVINCITY® 3%
+ H2O2
INVINCITY®
5%
5
FIRMNESS ❺
13%
In the dermis, collagen brings structural support to the
skin, and therefore its firmness.
The analysis of the general morphology shows that
INVINCITY® 3% improves the epidermal morphology
and increases collagen density in the papillary
dermis (ex-vivo).
Altered zone (non-viable)
3 to 4 cell layers
Altered zone (non-viable)
2 to 3 cell layers
Non altered zone (viable)
1 to 2 cell layers
Non altered zone (viable)
3 to 4 cell layers
Dense collagen network
Mildly dense collagen network
Skin + Polluting agents
(heavy metals + hydrocarbons)
EX-VIVO TEST CONDITIONS IN-VIVO TEST CONDITIONS
Human skin explants treated 5 days with INVINCITY® 3% versus placebo.
On day 5: a mix of pollutants (heavy metals + hydrocarbons) was added for 24h.
Parameters analyzed on day 6:
• General morphology of the skin (Masson’s trichrome stain)
• Expression of AhR (immunolabelling)
Double-blind study versus placebo on 15 urban women
(over 35 years old – average: 53 years old) with redness and dark spots on the face.
Twice-daily application on half face for 56 days:
• 1st side of the face: placebo cream
• 2nd side of the face: cream with 3% INVINCITY®
(1) Biological effect of various sulfated polysaccharides extracted from algae on skin barrier function through their impact on corneocyte maturation, Potter et al. ; 2011
D28
D56
-2%
-4%
D56
INVINCITY® 3%
7%
6%
5%
4%
3%
2%
1%
0%
-1%
-2%
-3%
-4%
-5%
550
TEWL variaon vs placebo
up to -13%
0%
D0
600
-14%
Anti-redness
30
Melanin content was measured with the Siascope® on a darkspot area.
After 28 days, INVINCITY® 3% decreases melanin content
by 8% compared to placebo (in-vivo).
0%
-1%
-3%
55%
43%
VEGF: Back to basic level aer oxidave stress
TEWL variaon (%)
AhR expression
40
63%
53%
50
900
Mature
corneocytes

60
0.1%
+2%
1%
76%
70
back to the baseline level.
VEGF ng/L
PROTECTS
80
2%
VEGF is a mediator of the vascular inflammation: it boosts the
vasodilatation and capillary permeability which lead to redness.
The VEGF modulation was evaluated on human keratinocytes
undergoing inflammation by oxidative stress (in-vitro).
From 0.5% of INVINCITY®, the VEGF concentration comes
CORNEOCYTES MATURATION
The first role of skin is to protect the body from external attacks.
A deficit of mature corneocytes leads to a dysfunctional barrier.
The impact of Fucoidans from Ascophyllum nodosum extract on the Cornified Envelope (CE)
maturation process was evaluated on reconstructed epidermal skin (ex-vivo; EpiskinTM model).
Native Fucoidans 5% increase the number of mature CE by 225%.(1)
90
4
ANTI-REDNESS ❹
2
BARRIER REINFORCEMENT ❷
The secret weapon
against POLLUAGING
3%
100
Hemoglobin content variaon (%)
Algues & Mer (A&M) innovates with an incredible active
ingredient: INVINCITY®.
It protects the skin from pollution from the inside,
and fights its damages, to prevent POLLUAGING®.
5
❺
% Inhibion enzyme acvity
Half of the world’s population lives in cities. Pollution is now
a major concern for consumers.
What is the impact on Earth? On my health?
And on my skin!?
Exposed to urban pollution, the skin becomes more sensitive.
It loses its brightness and ages prematurely.
This is « POLLUAGING® ».
Skin + Polluting agents
+ INVINCITY® 3%
Parameters analyzed:
• Redness and dark-spot evaluation with Siascope®
• TransEpidermal Water Loss (TEWL) measurement with Tewameter®
Results for the 10 best volunteers.
*Significant p<0.05; ** Significant p<0.01 ‐ Student’s t‐test
ALGUES & MER
OUESSANT ISLAND: OUTSTANDING PLACE
This fantastic environment is protected by many institutions,
like UNESCO (biosphere reserve).
A&M: THE FUCOIDANS EXPERT
INVINCITY® is extracted from Ascophyllum nodosum, harvested on the island before being
processed in our factory.
Fully in the sustainable way, Algues & Mer uses a hand cutting harvest method.
This provides regeneration stimulation and a quick new growth.
All our seaweeds on Ouessant Island are certified organic.
INVINCITY® is concentrated in High Molecular Weight (HMW) Fucoidans, over 100 kDa.
Naturally present in brown seaweeds only, Fucoidans are sulphated polysaccharides.
Main monomer is Fucose.
Fucoidans are well-known for many different benefits in cosmetics and medicine.
Each year, scientists find new properties out of these molecules, detailed in hundreds of
scientific publications.
Algues & Mer has a unique technical know-how to produce Fucoidans of different molecular weights.
Like hyaluronic acid, effects of Fucoidans on the skin depend on their molecular weights.
A&M Low Molecular Weight Fucoidans are reserved, with worldwide exclusivity, for a major brand.
The secret weapon against POLLUAGING®
TECHNICAL
INFORMATION
Appearance: Clear to cloudy liquid
INCI: Ascophyllum nodosum Extract, Sodium Benzoate,
Potassium Sorbate
Origin: Certified organic brown seaweeds of Ouessant Island
Dry matter: 6 to 7%
Solubility: Water soluble
Recommended dose: 3%
Processing:
• Emulsion: add INVINCITY® below 50°C, during the cooling
process.
• Lotion, serum: add INVINCITY® at any time,
in a pH range of 3.5 to 8.
APPLICATIONS
• Anti-pollution products
• Global anti-aging care
• Soothing care, anti-redness for sensitive skin
• Whitening, brightening and lightening products
• Concealer (CC cream...)
• After sun products
• Your next innovation!
Enjoy
Ouessant
unpolluted
fresh air!
Contacts & distributors list: www.algues-et-mer.com
©Algues & Mer - Argu Invincity - Mars 2016 ; Crédits photos: 123RF/ Gromovataya - Songquan Deng – Guniita ; Photo algues & Ouessant ©Algues & Mer
Factory and laboratory are located on Ouessant Island, 20 km off Brittany coast,
at the western tip of France, far from any source of industrial or agricultural pollution.
PROTECTS
Anti-pollution •
(AhR regulation)
Barrier reinforcement •
REPAIRS
• Anti-dark spots
• Anti-redness
• Higher collagen
density