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Transcription

tosoh bioscience
contents
EcoSEC SEC/GPC System
about us
contents
page [00 - 00]
EcoSEC GPC System
all-in-one system
page [01 - 01]
INcreased throughput and lower solvent cost
page [01 - 03]
Superior performance
page [04 - 10]
page [11 - 12]
page [13 - 13]
Optional UV Detector
EcoSEC GPC System Specifications
Applications
page [14 - 14]
page [15 - 15]
page [16 - 16]
HFIP Reproducibility
Modified polyurethane prepolymer analysis
Analysis of styrene and isoprene block copolymers before and after fluorination
GPC columns and calibration standards
TSKgel GPC columns
page [17 - 21]
PstQuick GPC polystyrene calibration standards
page [22 - 23]
TSKgel polystyrene calibration standards
page [24 - 24]
TOSOH BIOSCIENCE
Im Leuschnerpark 4, 64347 Griesheim, Germany
Tel: +49 (0)6155 70437-00 Fax: +49 (0)6155 83579-00
[email protected] www.tosohbioscience.de
B15I06A
2
Tosoh bioscience LLc
3604 Horizon Drive,
Suite 100
King of Prussia, pa 19406, USA
t + 49 (0) 6155 70437 00
f + 49 (0) 6155 83579 00
[email protected]
www.tosohbioscience.DE
t +1 484 805 1219
f +1 610 272 3028
[email protected]
www.separations.us.tosohbioscience.com
t +81 3 5427 5118
f +81 3 5427 5198
[email protected]
www.tosohbioscience.com
1
Tosoh bioscience gmbh
Im Leuschnerpark 4
64347 Griesheim
Germany
about us
With a global perspective.
TOSOH BIOSCIENCE GmbH, Separations Business Unit, Stuttgart,
is an acknowledged global leader in the field of bioseparations.
Established as TosoHaas in 1987, the original joint venture
between Tosoh Corporation of Japan and the Rohm and Haas
Company, USA, has become synonymous with advanced products
and quality support. In the year 2000, Tosoh Corporation acquired
a 100% controlling interest changing the name to TOSOH BIOSEP.
In the course of unifying all Tosoh affiliates, the new Brand Name
Tosoh Bioscience evolved. Today, the two branches, Bioseparations
and Diagnostics operate with the same name Tosoh Bioscience Separations Business Unit and accordingly Diagnostics Business
Unit. Tosoh manufacturing sites in Japan provide products to the
sales and support subsidiaries in the U.S. and Europe, ensuring full
global coverage. Tosoh has a long and successful history in manufacturing instruments for gel permeation chromatography (GPC) for
the Asian market. Based on the wide experience in GPC instrument
design and GPC column technology, Tosoh has developed the new
EcoSEC system to meet the market demands for high throughput,
semi micro GPC.
3
Tosoh corporation
3-8-2 Shiba, Minato-Ku
TOKYO 105-8623
japan
1
2
3
4
5
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Tosoh bioscience SHANGHAI CO. LTD.
ROOM 301, plaza b,
NO. 1289 yi shan road
xu hui district
SHANGHAI, 200233, CHINA
t +86 21 3461 0856
f +86 21 3461 0858
[email protected]
www.separations.asia.tosohbioscience.com
5
Tosoh asia Pte. LTD.
63 market street #10-03
Bank of singapore centre
Singapore 048942, singapore
t +65 6226 5106
f +65 6226 5215
[email protected]
TOSOH HISTORY
1935
Founding of Toyo Soda Manufacturing Co., Ltd.
1936
Operation of Nanyo Manufacturing Complex begInS
1971
Scientific Instruments Division formed, First GPC column using TSKgel developed by Tosoh
1974
High performance liquid chromatography column plant Is completed
1979
Tosoh develops TOYOPEARL media
1983
Tosoh develops Hydrophobic Interaction Media
1987
TosoHaas US operations formed in Montgomeryville
1989
TosoHaas GmbH operations formed in Stuttgart
1995
Tosoh Nanyo gel facility receives ISO 9001
2002/2003all Tosoh affiliated scientific & diagnostic system related companies in Europe are unified under the name TOSOH BIOSCIENCE
2008EcoSEC, THE 7TH GENERATION GPC SYSTEM IS INTRODUCED GLOBALLY
2010
Tosoh celebrates its 75th year in business with the opening of five new plants, and continued rapid expansion in china
2011
Tosoh Bioscience celebrates 40 Years of operation
2012
Tosoh Releases first TOYOpearl mixed-mode resin toyopearl mx-Trp-650M
2013
Tosoh releases A high capacity Protein A Chromatography resin
2014
Tosoh Bioscience GmbH celebrates its 25th anniversary in Stuttgart
2015
Tosoh bioscience successfully moves its sales & marketing offices to griesheim, darmstadt
DISCOVER the new high temperature GPC SYSTEM for high quality polymer analysis
Compact all-in-one HT-GPC system
Outstanding RI performance through dual flow Cell
Ultra High Temperature GPC up to 220°C
Heating transfer line for optional external detectors
Sample preparation system up to 220°C
Intelligent safety system with auto-locked doors
The EcoSEC-HT GPC System is distributed by Tosoh Bioscience GmbH. If You are interested in the EcoSEC-HT
GPC system or would like to get more information, please contact [email protected].
2
3604 Horizon Drive,
Suite 100
t + 49 (0) 6155 70437 00
f + 49 (0) 6155 83579 00
[email protected]
t +1 484 805 1219
f +1 610 272 3028
[email protected]
t +81 3 5427 5118
f +81 3 5427 5198
[email protected]
1
Im Leuschnerpark 4
64347 Griesheim
Germany
about us
semi micro GPC.
3
Tosoh corporation
TOKYO 105-8623
japan
1
2
3
4
5
4
ROOM 301, plaza b,
xu hui district
t +86 21 3461 0856
f +86 21 3461 0858
[email protected]
5
t +65 6226 5106
f +65 6226 5215
[email protected]
TOSOH HISTORY
1935
1936
1971
1974
1979
1983
1987
1989
1995
2010
2011
2012
2013
2014
2015
tosoh bioscience
contents
about us
contents
page [00 - 00]
EcoSEC GPC System
all-in-one system
page [01 - 01]
page [01 - 03]
page [04 - 10]
page [11 - 12]
page [13 - 13]
Applications
page [14 - 14]
page [15 - 15]
page [16 - 16]
TSKgel GPC columns
page [17 - 21]
page [22 - 23]
page [24 - 24]
TOSOH BIOSCIENCE
Tel: +49 (0)6155 70437-00 Fax: +49 (0)6155 83579-00
B15I06A
Tosoh bioscience ANALYSIS
INSTRUMENTS
1
GPC
EcoSEC
GPC System
All-In-One-System
Increased throughput
Unparalleled versatility
Semi-micro (4.6 mm ID) columns cut run time in
half, doubling your throughput
Autosampler for unattended operation
Stable RI baseline in THF within 2 hours of startup
Column switching valve
Easy to use, intuitive software
Optional UV detector
Optional Viscometer and Multi-Angle Light
Scattering detector
Optional 3rd party software allows system
control, data handling, and connectivity to
external detectors and other lab systems
Lower solvent cost
Low dead volume requires 85% less solvent
compared to conventional GPC systems
Unmatched baseline stability due to unique
dual flow RI design
Excellent retention time reproducibility due
to advanced temperature controlled pumps
Day to day, system to system, location to
location consistency
Increased Throughput and Lower Solvent
Costs
Minimal extra-column band broadening is required
to take full advantage of the highest efficiency GPC
columns. The EcoSEC GPC System is engineered to
minimize system dead volume. The semi-micro design
allows the use of GPC columns with smaller ID (4.6
mm) and shorter lengths (15 cm) such as the TSKgel
SuperMultiporeHZ columns. Together with a small stroke
volume pump and a 2.5 μL RI flow cell, the EcoSEC GPC
System allows accurate and precise molar mass measurements, particularly when benefiting from state-of-the-art
column technology.
2
GPC
EcoSEC
GPC System
table 1
Component
Description
Benefit
Semi-micro
design
The EcoSEC GPC System is engineered
for low volume by reduced tubing lengths,
low dead volume flow cells, and small
stroke pumps.
Maintains the efficiency of semi-micro
columns (4.6. mm ID x 15 cm). The result
is no loss of resolution at 1/6 the flow
rate and 50% of the run time compared to
conventional (7.8 mm ID x 30 cm) columns.
Control panel
Allows the system to be controlled
manually at the discretion of the operator.
Saves time by controlling a series of
operations without the use of the computer
or software.
Autosampler
100 sample capacity, 1 to 1,500 µL per
injection.
Automatic sample injection for unattended,
around the clock operation.
Purge unit and
degasser
20 and 40 mL solvent volume; variable
degassing capactiy (for semi-micro or 30
cm column).
Saves time with rapid solvent changes
via purge valve eliminating solvent
replacement and other time-consuming
manual operations.
Temperature
controlled
pumps
Pump heads and solvent lines are
maintained at a constant temperature.
Improves baseline stability by removing
the effect of temperature fluctuations.
This results in consistent and accurate
flow rates and reproducible molar mass
determinations.
Column oven
Engineered for precise (+/- 0.02°C) column
temperature; oven can accomodate up to
8,30 cm length columns.
Constant column temperature ensures
precise and reproducible molar mass
determinations.
RI detector
Low dead volume flow cell, 2.5 µL. Solvent
flows through a separate reference cell.
Enhanced baseline stability from dual flow
cell RI detector.
UV detector
(optional)
Low dead volume flow cell, 2 µL.
Wavelength range from 195-350 nm.
Open for
polymers.
Light scattering
detector (optional)
Up to 7 angle MALS design.
Absolute molar mass determination on the
widest range of polymer sizes.
Viscometry
detector (optional)
Unique 80/20 split between sample and
reference flow paths.
Reduces run times by up to 50% compared
to 50/50 split flow path detectors.
measuring
UV-absorbing
INSTRUMENTS
3
GPC
EcoSEC
GPC System
As shown in Figure 1, when run on the EcoSEC GPC System,
the TSKgel SuperMultiporeHZ-N (4.6 mm ID × 15 cm)
column achieves separation efficiency equivalent to that
of a conventional high-speed column (7.8 mm ID × 30 cm),
but analysis time is reduced to half that of a conventional
column and one-sixth the amount of solvent is consumed.
Figure 2 shows an example of an oligomer (A-500) separation using four TSKgel SuperHZ2000 GPC columns
in tandem performed using an EcoSEC GPC System
and a competitive GPC system. A faster analysis and
improved resolution is achieved with the EcoSEC GPC
System as a result of the advanced engineering design
of the system.
The combination of the EcoSEC GPC System and semimicro columns provides significant solvent related cost
savings while doubling sample throughput without compromising resolution. As shown in Table 2, the solvent related
cost savings are extraordinary for samples requiring expensive solvents such as hexafluoroisopropanol.
Figure 1
Figure 2
90
40
Detector response (mV)
70
A
50
30
10
EcoSEC GPC System
Competitor GPC System
30
B
-10
10
30
20
Retention time (minutes)
20
40
14
15
16
17
18
Comparing semi-micro and conventional gpc columns
Column: A. Conventional column, 7.8 mm ID x 30 cm x 4
B. TSKgel SuperMultiporeHZ-N, 4.6 mm ID x 15 cm x 4
Sample: poly(teramethylene ether glycol) (PTMEG 650), 10 µg/µL;
Mobile phase: THF; Flow rate: A. 1.0 mL/min, B. 0.35 mL/min;
Detection: RI; Temperature: 40°C; Injection vol.: A. 50 µL, B. 10 µL
comparison of resolution of a semi-micro column
run on an EcoSEC GPC system and a conventional
gpc system
Column: TSKgel SuperHZ2000, 4. 6mm ID x 15 cm x 4; Mobile phase:
THF; Flow rate: 0.3 5mL/min; Detection: RI; Temperature: 40°C;
Injection vol.: 10 µL; Sample: styrene oligomer (A-500), 0.2 mg/mL
table 2
Annual Solvent Cost Saving with Semi-Micro Columns and the EcoSEC GPC System
SolventCompetitive GPC SystemEcoSEC GPC SystemSavings
THF cost (€ 30/L)/year
3.600
1.1882.376
THF disposal cost (€ 30/L)/year
3.600
1.1882.376
HFIP* cost (€ 1.500/L)/year
HFIP disposal cost (€ 60/L)/year
* THF: tetrahyrofuran; HFIP: hexafluoroisopropanol
180.000
59.400120.600
TSKgel SuperHZ2000, 4.6mm ID x 15cm, × 4
7.200
2.3764.824
Mobile phase:
Flow rate:
Detection:
Temperature:
Injection vol.:
THF
0.35mL/min
RI
40°C
10µL (0.2mg/mL)
4
GPC
EcoSEC
GPC System
Superior Performance
Unmatched Baseline Stability
Refractometer
Dual flow cell design
Continous correction of RI baseline drift due
to solvent instability
Improved molar mass precision and accuracy
Rapid baseline stability at startup
Basic Principle of Refractive Index Detection
The most common type of differential refractive index detector is a deflection-type detector employing the principles of
Snell’s law of refraction. In this type of detector, light emitted
from a source is transmitted through the flow cell of the
RI detector and strikes a detector element. The flow cell is
constructed in such a way that there are two separate, triangular pyramid shaped compartments (sides): (1) the reference
side, consisting of stagnant pure solvent; and (2) the sample
side, containing a flowing stream of analyte in the same
solvent as in the reference side. As the light passes through
the reference side into the sample side, the direction in which
the light is travelling is changed, e.g., the path is bent. The
amount of bending that takes place depends on the nature
of the flow cell, the wavelength of the light being used, and
the temperature and the concentration of analytes in the cell.
Figure 3
Reference cell
containing
only solvent
The light then strikes a mirror and reflects back through
the cell to two photodiodes mounted on a single chip. The
two photodiodes will produce equal signals if the contents
of the reference and sample sides have the same refractive
indices as each other (Figure 3). In contrast, if the reference
and sample sides have different refractive indices, a voltage difference will result between the photodiodes because
the two photodiodes detect the difference in light intensity
due the bending of the light beam, as shown in Figure 4.
The difference in refractive indices between the two sides
produces a voltage difference proportional to the concentration of the analyte in solution.
Figure 4
Sample cell
containing
only solvent
Depiction of RI detector flow cell when the contents
of the referecne and sample sides have the same
refractive indices as each other
Reference cell
containing
only solvent
Sample cell
containing
solvent plus
analyte
Depiction of RI detector flow cell when the contents
of the reference and sample sides have different
refractive indices
INSTRUMENTS
5
GPC
EcoSEC
GPC System
Dual Flow Refractive Index Detector
The refractive index detector in the EcoSEC GPC System
is unlike any other refractive index detector on the market
due to its unique dual flow design. The EcoSEC GPC System RI flow cell is constructed in such a way that there are
two sides: (1) the reference side, containing a flowing stream
of pure solvent; and (2) a sample side, containing a flowing
stream of analyte in the same solvent as in the reference side
(Figure 5).
Another benefit of the dual flow cell is rapid attainment of
baseline stability when the instrument is first started, as
purging is not required. A stable baseline can be achieved
by flowing only 50 mL of solvent through the instrument.
Additionally, the reference side mobile phase can be sent to
waste or recycled back to the solvent bottle.
The unique dual flow design of the EcoSEC GPC System
results in superb RI baseline stability and reduced RI baseline drift. In a conventional RI detector, over time, the refractive index of the stagnant pure solvent in the reference side
will slowly change and the two photodiodes will no longer
produce equal signals, thus the contents of the reference
and sample sides have different refractive indices and will
produce a voltage difference similar to that of an analyte
in solution. For example, the refractive index of THF slowly
alters over time, due to the buildup of peroxide-related compounds, resulting in baseline drift (Figure 6). The dual flow
design of the RI detector in the EcoSEC GPC System compensates for the changes in refractive index of the solvent
over time by continuously flowing pure solvent through the
reference side of the flow cell.
The EcoSEC GPC System offers unmatched baseline stability
because it is the only GPC system which uses a dual flow
refractive index detector and temperature controlled pumps.
Baseline stability is essential for the accurate calculation of
polymer molar mass averages. For example, computer simulations predict a polymer with a polydispersity index (PDI)
of 5 will have an 18% error for Mz if baseline instability leads
to a 4% error in peak width determination. In addition, a 2%
uncertainty in baseline height will result in a 20% error in
Mz1.
Tcjir, W.J.; Rudin, A.; and Fyfe, C.A.; Journal of Polymer Science:
Polymer Physics Edition, Volume 20, Issue 8, 1443-1451
1
Figure 5
Figure 6
Reference cell
containing
degraded THF
(stagnantt liquid)
Reference cell
containing
flowing solvent
Sample cell
containing
only solvent
Depiction of Dual flow RI detector in the ecosec system,
showing the compensation of the changes in refractive
index of the solvent over time
Sample cell
containing
only solvent
Depiction of RI detector flow cell showing the effects
of thf degradation in the stagnant reference side of a
conventional gpc system
Column:
6
Eluent:
Detector:
Temp.:
Flow rate:
Sample:
Number of Injections:
TSKgel SuperHZ-H, 6µm, 4.6mm x 15cm x 1
THF
RI
40°C
0.35mL/min,
dicyclohexylphthalate
5
GPC
EcoSEC
GPC System
A study was done to demonstrate the superb baseline
stability of the EcoSEC GPC System compared to that of two
conventional GPC systems over a five hour time period. As
shown in Figure 7, five consecutive injections of polystyrene standards with run times deliberately extended to one
hour without auto zeroing the detectors between injections,
resulted in an extremely stable baseline with low baseline drift
on the EcoSEC GPC System and a significantly drifting baseline on the two conventional GPC systems. In comparison
50 to the conventional GPC systems, the EcoSEC GPC System
has both a lower baseline drift and a better signal to noise
ratio.
Figure 7
50
40
30 Comparison of Baseline Stability
30
mV
40
Five injections of dicyclohexyl phthalate were made on the
20 EcoSEC GPC System and a conventional GPC instrument
with a stagnant reference cell. The chromatograms were
10 overlaid and are shown in Figures 8A and 8B.
With the EcoSEC GPC System, superposition of five con0 secutive chromatograms shows negligible baseline drift, as
0
4 repeated with
6 a competicompared
to the2same experiment
Minutes
tor’s GPC system having a non flow-through reference cell.
20
10Comparison of baseline drift of the dual flow refRactive index detector of the ecosec gpc system and two
conventional gpc systems
0
Column: TSKgel SuperMultiporeHZ-M, 4.6 mm ID x 15 cm x 2;
5
6
7
1
2
3
0
4
Sample: polystyrene standards, PStQuick MP-M series; Mobile
phase: THF; Flow rate: 0.35Minutes
mL/min; Detection: RI; Temperature:
40°C; Injection volume: 10 µL
Figure 8
50
comparison of baseline stability
A. EcoSEC GPC System
40
Column: TSKgel SuperMultiporeHZ-M, 4.6 mm ID x 15 cm;
Sample: dicyclohexyl phthalate; Mobile phase: THF; Flow rate:
0.35 mL/min; Detection: RI; Temperature: 40°C; Inj. volume: 10 µL
30
20
10
0
0
50
2
4
6
B. Competitor GPC System
40
mV
30
20
10
0
0
1
2
3
4
5
6
7
INSTRUMENTS
7
GPC
EcoSEC
GPC System
Comprehensive Temperature Control
Elution Time Precision
To assess the influence of environmental conditions within
the laboratory on solvent flow, a study was done in which
the EcoSEC GPC System and a conventional GPC system
were placed in a chamber where the temperature was
cycled between 23°C and 26°C. A series of sixty injections of
polystyrene were made over a time period of ten hours. For
each instrument the elution volume at peak maximum was
measured; the resulting data is shown in Figure 9 below. The
elution volume drift of the EcoSEC GPC System was about
20% lower than that of the conventional GPC system.
Figure 10 demonstrates the superiority of the EcoSEC GPC
System for the determination of weight average molar masses. Figure 11 shows a comparison of Mw reproducibility for a
sample injected 10 times a day for 5 days on the EcoSEC GPC
System compared to a conventional GPC. The reproducibility
of the EcoSEC GPC System was superior by a factor of 3 to
that of the conventional GPC system.
Figure 10
The results shown demonstrate that the engineering design
concepts of the EcoSEC GPC System result in a high degree
of reproducibility of elution times and molar mass determinations.
1.00
Polycaprolactone MW = 15,000g/mol
%CV
Mw Precision
Molar mass averages can be affected by changes in the
environment and measuring conditions. Generally, these
variations are the result of one or more factors including
flow rate reproducibility, baseline drift and injection reproducibility. In addition to controlling column temperature,
Tosoh engineers added temperature control for both pumps
and inlet and outlet tubing on the EcoSEC GPC System to
deliver top GPC analysis performance.
Poly(vinyl chloride-co-vinyl acetate) MW = 24,000g/mol
0.80
0.60
0.40
0.20
0.00
Day 1
Day 2
Day 3
Day 4
Day 5
Day-to-day
Reproducibility
CV value less than 0.2% a day.
CV value less than 0.4% on different days.
reproducibility of MW Analysis
Sample: poly (vinyl chloride-co-vinyl acetate); Mobile phase: THF;
Flow rate: 0.35 mL/min ; Temp.: 40 °C; Detection: RI; Inj.volume: 10 µL
Figure 9
Figure 11
24
3.03
23
10
20
30
40
Injection number
50
22
EcoSEC GPC System Elution volume %CV=0.070
Conventional GPC System Elution volume %CV=0.23
Temperature of Chamber
Mobile Phase delivery reproducibility with ambient
temperature changes
Sample: polystyrene standard; Mobile phase: THF; Flow rate: 0.35
mL/min, Detection: RI; Temperature: 40°C; Inj.volume: 10 µL
Temperature was cycled 23°C-26°C in the testing chamber.
Conventional GPC
4.5
4
EcoSEC GPC System
3.5
%CV
25
3.04
Room temperature (°C)
Retention volume (mL)
at peak maximum
26
3.06
3.01
5
27
3.08
3
2.5
2
1.5
1
0.5
0
Day 1
Day 2
Day 3
Day 4
Day 5
Day-to-day
Reproducibility
Comparing MW reproducibility of the EcosEc gpc system
and a conventional gpc system
Sample: poly (vinyl chloride-co-vinyl acetate); Mobile phase: THF;
Flow rate: 0.35 mL/min ; Temp.: 40 °C; Detection: RI; Inj.volume: 10 µL
8
GPC
EcoSEC
GPC System
System-to-System Reproducibility
TABLE 3
Often measurements can be reproduced using the same
equipment but results differ when an instrument from the
same or another manufacturer is used. Among the systemspecific factors which can influence the results of GPC analysis, fluctuations in elution time, in particular, can have a
significant effect.
Site-to-Site reproducibility
Mn MWWZ
Site A
13,800
29,800
53,700
Site B
13,700
29,900
54,300
A study was performed using a polydisperse poly(vinyl chloride-co-vinyl acetate) sample run on four different EcoSEC
GPC Systems by different operators to assess system reproducibility. The results are shown in Figure 12. The highprecision of the EcoSEC GPC System results in minimal variation among instruments and from day-to-day.
Site C
13,600
29,800
53,200
Site D
13,700
30,200
54,100
Average13,700
29,900
53,800
Deviation70
160
420
Site-to-Site Reproducibility
%CV0.52 0.55 0.78
To test site reliability, a round-robin study was undertaken
in which the same polydisperse poly(vinyl chloride-co-vinyl
acetate) sample was run on EcoSEC GPC Systems located at
four different sites. The results are displayed in Table 3.
Reproducibility from system-to-system and location-tolocation is exceptional with the EcoSEC GPC System. Coefficients of variations for all mass determinations were all well
below 1%. Because of the high instrument-to-instrument
reproducibility of the EcoSEC GPC System, methods developed at one location, e.g., an R&D laboratory, can be reliably
transferred to a second site, e.g., a QC lab at a manufacturing
site, and so on.
For EcoSEC GPC Systems, 4 operators, 4 column sets,
4 conditions, 4 locations
Sample: poly (vinyl chloride-co-vinyl-acetate); Mobile phase: THF;
Flow rate: 0.35 mL/min, Detection: RI; Temperature: 40°C; Inj.volume:
10 µL
Average of values measured with each instrument (n=10).
System 1
System 2
System 3
Day 1
Day 2
Day 3
Day 4
Day 5
AVG
Day 1
Day 2
Day 3
Day 4
Day 5
AVG
Day 1
Day 2
Day 3
Day 4
Day 5
AVG
10
9
8
7
6
5
4
3
2
1
0
Day 1
Day 2
Day 3
Day 4
Day 5
AVG
Figure 12
System 4
Four EcoSEC GPC Systems, 4 operators,
4 column sets, 4 conditions, one location
Day-to-Day reproducibility
Column: TSKgel SuperMultiporeHZ-M, 4.6 mm ID x 15 cm x 2; Sample: poly (vinyl chloride-co-vinyl acetate); Mobile phase: THF; Flow
rate: 0.35 mL/min ; Temp.: 40 °C; Detection: RI; Inj.volume: 10 µL
INSTRUMENTS
9
GPC
EcoSEC
GPC System
Rapid Column Switching
The EcoSEC GPC System contains two pumps: a sample
pump to deliver sample and solvent through the analytical
column and the sample side of the RI detector flow cell and
a reference pump to flow solvent (via a reference column) to
the reference side of the RI detector flow cell. By installing
an optional column switching valve and replacing the
reference column with another analytical column, an analysis
can be performed on column 1 while equilibrating column 2.
After switching the valve, column 2 becomes the measurement column while column 1 will be in the flow path to the
reference side of the RI detector flow cell.
Since the column switching valve changes column sets
while the oven door remains closed and switches to an
already equilibrated column set, a stable baseline is rapidly
established.
Column switching valve
Reduce column switching time
Easily switch between low MM and
high MM range columns
Eliminate temperature related base
line drift following column change
Figure 13
Column #1 (Analytical)
UV Detector
Sample
Pump
Injection
Reference
Cell
RI Detector Sample
Cell
Reference
Pump
Column #2 (Reference)
Solvent
A
Waste
Column #1 (Reference)
UV Detector
Sample
Pump
Injection
Reference RI Detector Sample
Cell
Cell
Reference
Pump
Column #2 (Analytical)
Waste
Solvent
B
A.) flow path with column 1 as the analytical column B.) flow path with columns 2 as the analytical column
10
GPC
EcoSEC
GPC System
Comparison of Time to Baseline Stability with and
without the Column Switching Valve
On the EcoSEC GPC System the RI baseline is considered
stabilized when the drift in signal is 1 x 10-7 RIU/hr or less
(based on THF at a flow rate of 1.0 mL/min). When a new set
of columns is manually placed on the EcoSEC GPC System
and the flow rate is started, the RI baseline stabilizes after
80 - 90 minutes. When a new column set is brought online
using the column switching valve, the baseline stabilizes
after 15 minutes.
(Experimental conditions: THF, 35°C, 0.35 mL/min, 20 minute
warm-up at 50% flow rate). Figure 14 clearly demonstrates
the 65 – 75 minute savings in time required to reach a stable
baseline when the columns are switched using the column
switching valve compared to manually changing columns.
Figure 14
Stable baseline
achieved
within 90 minutes
Stable baseline achieved
Overlay of refractive index detector signals during equilibration following a column change using the column
switching valve (blue) and withou use of the columns switching valve (red)
INSTRUMENTS
11
GPC
Configuration options
UV Detector
Variable UV; 195 – 350 nm
Semi-micro flow cell (2 µL)
Factory installed option
The optional UV detector is variable from 195 to 350 nm
and the detector flow path and electronics are optimized for
the use of semi-micro columns. The volume of the flow
cell is reduced to 2 µL and the shortest time constant is
0.5 seconds.
Copolymer Analysis
The EcoSEC GPC System equipped with both RI and UV detectors can be used to determine the structural composition
of an unknown copolymer, in which the copolymer contains
one UV visible and one non-UV visible component. At least
one copolymer of known composition must be available to
create a copolymer calibration curve. The final result is a
plot of the structural composition at each molar mass. This
composition curve overlaid on the chromatogram, as seen in
Figure 15, can be generated using the EcoSEC GPC Workstation Software. The software allows for the creation and
use of separate UV and RI specific calibration curves while
correcting for the inter detector delay volume.
100
90
80
70
60
50
40
30
20
10
0
8 x 104
6 x 104
RI
4 x 10
4
UV
2 x 104
0
% Copolymer
Differential distribution
Figure 15
5
Log molar mass
Copolymer Analysis of Polystyrene-b-Polybutene
Column:TSKgel SuperMultiporeHZ-M, 4.6 mm ID x 15 cm x 2; Mobile phase: THF; Flow rate: 0.35 mL/min, Detection: RI, UV@254 nm;
Temperature: 40˚C; Injection vol.: 10 µL; Samples: PS-b-PB, 0.2 wt%
12
GPC
Configuration options
table 4
UV Detector Specifications
UV Detector Specification
System Dual beam, single flow cell
Light source Deuterium lamp
Wavelength range 195 to 350 nm
Wavelength accuracy ± 2 nm
Bandwidth 8 nm
Range (FS) 0.5, 1, 2, 4 AU/1 V
Response 0.5, 1.0, 3.0 seconds
Drift 3 x 10-4 AU/h (254 nm, air in cell, response: 1.0 s)
Noise 2.5 x 10-5 AU/h (254 nm, air in cell, response: 1.0 s)
Flow cell volume 2 µL
Safety mechanism Liquid leakage sensor; lighting time monitoring
INSTRUMENTS
GPC
EcoSEC
GPC System
Ecosec Specifications
PumpSpecification
Flow rate
0.010 to 2.000 mL/min in 0.001 mL/min steps
Accuracy
+/- 2%
Precision
+/- 0.2%
Maximum pressure
25 MPa or 3,500 psi
Safety features
Liquid supply stops if pressure rises above the upper limit or drops below
the lower limit, Plunger drive count monitoring, Pan for liquid leakage
Stroke volume
7.51 μL
Auto-injector
Injection volume
1 to 1,500 μL in 1 μL increments
Number of samplesAir detection in wash solution and sample solution, Needle lock when the
sample table is not loaded, Monitoring of 6-way and 4-way valve rotations
Column Oven
Temperature rangeAmbient plus 10°C to 60°C
Capacity
7.8 mm ID x 30 cm x 8 columns
Accuracy
+/- 0.5°C
Precision
+/- 0.2°C
RI Detector
Type
13
Bryce or dual flow type, Tungsten light source (1.00-1.80 RI range)
OpticsDeflection
Cell volume
2.5 μL
Cell pressure limit
0.5 MPa
Noise
2 x 10-9 RI units (RIU)
Drift
1 x 10-7 RIU/h (THF, 1.0 mL/min)
Dynamic range
+/- 2.5 x 10-4 RIU
Temperature controlOff, 35°C, 40°C, 45°C
Analog out
For connection to third party light scattering and viscometry detectors
Safety features
Leak sensor and thermal fuse for circuit block
Instrument
Dimensions
680 (W) x 500 (D) x 550 (H) mm = 2.2’ x 1.6’ x 1.8’
Weight
95 kg = 210 lbs
14
GPC
Applications
Dr. Li Jia and co-workers at the University of Akron are investigating different synthetic routes for the formation of polypeptoids with alternating block structures. Highly reproducible
data is needed to obtain subtle molar mass distribution
trends from the various synthetic routes. The EcoSEC GPC
System and a set of TSKgel mixed-bed columns were used
successfully to obtain high quality molar mass distribution
(MMD) data of a series of Dr. Jia’s block poly-ß-alkylalanoids
with hexafluoroisopropanol (HFIP) as the mobile phase in
under 15 minutes.
As shown in Table 5, percent standard deviations are more
than 10 x lower than values previously reported for polyamides in HFIP2.
Percent relative standard deviation of the polydispersity
index (PDI) ranged from 0.1 to 0.5%, permitting one to
report PDIs within three significant figures. The high
precision of the EcoSEC GPC System allows for the detailed
study of polymerization reactions.
Sample chromatograms from 4 selected poly-ß-alkylalanoid
samples run on an EcoSEC GPC System using two TSKgel
GMHHR-M, 5 µm, 4.6 mm ID x 15 cm columns are shown in
Figure 16. Sample profiles display very little tailing and no
baseline drift, allowing for highly precise data not available
with conventional systems. All samples, with the exception
of (C)40, contain almost symmetrical, narrow polymer profiles eluting around 6 minutes. The shoulder seen in (C)40
is indicative of another population of a high MW polymer
component in the sample.
table 5
Averaged Values from Three Consecutive Injections and the Percent Relative Standard Deviations
SampleaMnbMwbPDIb
Rel std devRel std devRel std dev
(A)10(B)40
26,500 ± 10
0.04%
30,300 ± 30
0.11%
1.14 ± 0.01
0.09%
(A)60(B)20
33,300 ± 170
0.52%
40,700 ± 28
0.07%
1.22 ± 0.01
0.50%
(A)40(B)40
48,700 ± 220
0.45%
60,900 ± 160
0.26%
1.25 ± 0.01
0.10%
(C)40
30,100 ± 50
0.18%
36,400 ± 140
0.37%
1.21 ± 0.01
0.39%
a.
Block lengths were determined by Dr. Jia from independent measurements. Chemical composition of blocks A, B and C will be published by L. Jia.
b.
Molar mass data were obtained from a PMMA calibration curve. Molar mass averages given in the table are averages of three sequential injections per sample.
Based on block lengths, MMD are significantly overestimated.
(A)60(B)20
60
40
20
4
6
80
8
10
12
Figure 16
(A)10(B)40
60
40
20
4
6
8
10
12
(B)40(C)40
80
60
40
20
4
6
8
10
12
80
(C)40
60
40
20
4
6
8
10
12
Poly-b-alkylalanoid samples
TSKgel GMHHR-M, 5 µm, 4.6 mm ID x 15 cm x 2 packed in HFIP; Samples: selection of poly-b-alkylalanoid samples
Mobile Phase: HFIP containing 5 mmol/L sodium trifluoroacetate; Flow rate: 0.35 mL/min; Detection: RI; Temperature: 40˚C Inj. vol.: 10 µL
2
Robert, E. C.; Bruessau, R.; Dubois, J.; Jacques, B.; Meijerink, N.; Nguyen, T. Q.; Niehaus, D. E.; Tobisch, W. A. Pure Appl. Chem. 2004, 76, 2009–2025
INSTRUMENTS
15
GPC
Applications
Modified Polyurethane Prepolymer Analysis
Figure 17
An EcoSEC GPC System was used to analyze an isocyanate
modified polyurethane prepolymer with residual DMSO. As
shown in Figure 17, separation of the sample by GPC results
in ten positive chromatographic peaks and two negative
chromatographic peaks. The first nine chromatographic
peaks correspond to components of the modified polyurethane prepolymer while the two negative chromatographic
peaks are indicative of the solvent, THF. The latest eluting peak
is a result of the residual DMSO present in the sample and is
retained by a non-SEC retention mechanism, as it elutes
after the void volume of the column.
The molar mass averages Mn, Mw, and Mz of the sample,
given in Table 6, were determined via a polystyrene relative
calibration curve. The sample was found to have a weight
average molar mass Mw ranging from 4,199 to 178 g/mol.
The polydispersity index (PDI) shown in Table 6 for the
entire sample, e.g., peaks 1 through 9, was 2.26 while the
individual components of the polyurethane prepolymer had
PDI values ranging from 1.01 to 1.09. From the PDI values
it can be concluded that collectively the sample is polydispersed with respect to molar mass, but the nine visible
components within the sample are virtually monodispersed
with respect to molar mass.
Modified polyurethane prepolymer sample
Column: TSKgel SuperH3000, 6.0 mm ID x 15 cm x 2; Sample:
modified polyurethane prepolymer, 10 mg/mL ; Mobile phase: THF;
Flow rate: 0.30 mL/min; Detector: RI; Temperature: 35°C; Injection
volume: 20 μL
table 6
Molar Mass Averages and Polydispersity Index for Modified Polyurethane Prepolymer Sample in THF at 0.3 mL/min
Peak Mn MwMzPDIa
1 4,199 ± 46b 4,606 ± 67 5,214 ± 109 1.09 ± 0.01
2 2,643 ± 19 2,655 ± 18 2,667 ± 18 1.01 ± 0.01
3
2,011 ± 16
2,024 ± 16
2,038 ± 16
1.01 ± 0.01
4
1,387 ± 10
1,403 ± 10
1,418 ± 10
1.01 ± 0.01
5
798 ± 4
808 ± 5
817 ± 5
1.01 ± 0.01
6
551 ± 9
554 ± 9
557 ± 9
1.01 ± 0.01
7
391 ± 9
394 ± 9
397 ± 10
1.01 ± 0.01
8
278 ± 3
280 ± 3
282 ± 3
1.01 ± 0.01
9
178 ± 1
181 ± 1
183 ± 1
1.01 ± 0.01
All
676 ± 9
1,531 ± 31
2,873 ± 83
2.26 ± 0.02
a
PDI = Mw/Mn
b
Standard deviations from six injections
16
GPC
Applications
Analysis of Styrene and Isoprene Block Copolymers Before and After Fluorination
Dr. Jimmy Mays’ group from the Department of Chemistry
at the University of Tennessee, Knoxville, is synthesizing
and characterizing the bulk morphology of fluorinated and
sulfonated block copolymers. Well-defined block copolymers
of sulfonated polystyrene-b-fluorinated polyisoprene (sPS-bfPI), Figure 18, were synthesized by anionic polymerization
followed by fluorination and sulfonation3.
The EcoSEC GPC System, equipped with TSKgel SuperMultipore® columns, was then used to determine the numberaverage molar mass, Mn, and the polydispersity index, PDI, of
sPS-b-fPI, as well as that of the precursor polymer (PS-b-PI),
Table 7. As seen in Figure 19, complete analysis of sPS-b-fPI
was obtained in less than 10 minutes with excellent resolution
using the EcoSEC GPC System.
TABLE 7
Figure 18
x
Number-Average Molar Mass, Mn, and the Polydispersity
Index (PDI) of sPS-b-fPI and the Precursor Polymer (PS-b-PI)
PS-b-Pl sPS-b-fPl
H
z
y
F
F
Seriesa Mn(SEC) PDI Mn(SEC) PDI
1
2.1 x 104 1.04 2.5 x 104 1.08
2
4.6 x 104 1.03 6.0 x 104 1.05
SO3(H, Na)
a
series 1 in acid form; series 2 in Na form
Structure of Sulfonated Polystyrene-b-Fluorinated
Polyisoprene (sPS-b-FPI)
Figure 19
A
B
Detector response
PS-b-f P I:
Mn=2.5 x 104, PDI=1.08
PS-b-f P I:
Mn=6.0 x 104, PDI=1.05
PS-b-P I:
Mn=4.6 x 104, PDI=1.03
PS-b-P I:
Mn=2.1 x 104, PDI=1.04
PS:
Mn=9.5 x 103, PDI=1.05
7.0
7.5
PS:
Mn=1.0 x 104, PDI=1.05
8.0
8.5
9.0
9.5
10.0
7.0
7.5
8.0
8.5
9.0
9.5
10.0
Sulfonated polystyrene-b-fluorinated polyisoprene precursor samples
TSKgel SuperMultiporeHZ-M, 4.6 mm ID x 15 cm x 1; Samples: A. series 1, table 3 B, series 2, table 3; Mobile Phase: THF; Flow rate:
0.35 mL/min; Detection: RI; Temperature: 35˚C Inj. vol.: 20 µL
Wang, X.; Hong, K.; Baskaran, D.; Goswami, M.; Sumpter, B.; Mays, J. Soft Matter, 2011, 7, 7960
3
INSTRUMENTS
17
GPC
TSKgel columns and
standards
TSKgel GPC Columns
Semi-micro Columns
Tosoh introduced its first line of GPC columns in 1971. Ever
since, Tosoh scientists have made important contributions to
advances in polymer analysis by developing state-of-the-art
GPC columns for the most demanding applications.
Semi-micro columns are the TSKgel columns of choice for
use with the EcoSEC GPC System. They are referred to as
such since their dimensions are smaller than conventional
columns in terms of internal diameter as well as in length:
4.6 mm or 6 mm ID x 15 cm vs. 7.8 mm ID x 30 cm.
GPC columns for polymers soluble in organic
solvents
Semi-micro columns* (4.6 or 6.0 mm ID x 15 cm)
TSKgel SuperMultiporeHZ columns
TSKgel SuperHZ columns for ultra-low adsorption
TSKgel SuperH columns for low adsorption
Conventional columns (7.8 mm ID x 30 cm)
TSKgel HXL columns for ultra-low adsorption
TSKgel HHR columns for low adsorption;
max temp. 140°C
*EcoSEC GPC System recommended columns
GPC columns for polymers soluble in polar
organic solvents
Semi-micro columns* (6.0 mm ID x 15 cm)
TSKgel SuperAW columns
Conventional columns (7.8 mm ID x 30 cm)
TSKgel Alpha columns
GPC columns for polymers soluble in aqueous
solvents
Semi-micro columns* (6.0 mm ID x 15 cm)
TSKgel SuperMultiporePW columns
18
GPC
TSKgel columns and
standards
Multi-pore Technology
Prior to the introduction of TSKgel SuperMultiporeHZ
columns, scientists separating polymers with a wide range
of molar masses were left with two options. One option
is to use multiple columns of different pore sizes linked
together in series. A second is to use a column packed with
a mixed-bed resin of different pore sizes at an optimized
mix ratio. However, problems can occur with both of these
methods, which include distortion of the chromatogram or
deviations between the actual calibration curve and the
calibration curve approximated from data obtained from the
molar mass standards.
As is shown in Figure 20, a novel approach to solve this
problem was developed by Tosoh scientists and is incorporated in TSKgel SuperMultiporeHZ columns. These columns
are packed with small particles of uniform size synthesized
with a broad distribution of pore sizes. This novel approach
creates a linear calibration curve within each particle. Therefore, columns with an extended linear calibration curve
can now be prepared without mixing particles of different
pore sizes. Their small ID (4.6 mm ID) and length (15 cm)
reduces solvent consumption, results in quick run times, and
offers high throughput capabilities.
Figure 20
Strategies for wide range separation using Size Exclusion Chromatography
Conventional Strategy
Large Pore
Medium Pore
Connect columns with
different grades of packings
(TSKgel G5000H+G4000H+G2000H)
New Strategy
Small Pore
Multiple Pore Size
Blend (mixed bed) packings
of different grades
(TSKgel GMH series)
Pure packings
with multi-pore size distribution
(TSKgel MultiporeHXL column)
Graphical Representations Illustrate the Multi-Pore Particle Synthesis Technology
INSTRUMENTS
19
GPC
TSKgel columns and
standards
Figure 21
TSKgel SuperMultiporeHZ Columns Packed with Monodisperse Particles
Figure 21 shows the monodispersity of the particle size distribution of TSKgel SuperMultiporeHZ columns compared to a conventional
mixed-bed column.
Figure 22
15
TSKgel SuperMultiporeHZ-M
A.
TSKgel SuperHZ
5
B.
-5
5
10
15
20
25
Comparison of TSKgel SuperMultiporeHZ-M and TSKgel SuperHZ Columns for the Separation of Acrylic Resin
Figure 22 demonstrates that inflection points are no longer observed with columns packed from particles prepared by multi-pore technology.
20
GPC
TSKgel columns and
standards
TSKgel SuperMultiporeHZ Columns
TSKgel SuperMultiporeHZ columns combine ultra high performance GPC columns with low dead volume (4.6 mm ID
x 15 cm) and feature multi-pore particles with a wide pore
size distribution. The use of the multi-pore technology
ensures that the calibration curves have excellent linearity.
There are three columns available within the TSKgel
SuperMultiporeHZ columns, each with a different particle
size, separation range, and exclusion limit. These columns
can separate and characterize polymers within a wide molar
mass range.
Figure 23
As demonstrated in Figure 23, the TSKgel SuperMultiporeHZ
columns have a highly linear calibration curve with a
shallow slope, which indicates excellent reproducibility
across various average molar mass values.
The TSKgel SuperMultiporeHZ-N columns provide the same
or higher resolution at a much shorter analysis time than
multiple columns linked together, as shown in Figure 5.
Figure 24
108
90
TSKgel SuperMultiporeHZ-N
107
TSKgel SuperMultiporeHZ-M
70
TSKgel SuperMultiporeHZ-H
106
10
MW
5
104
103
102
101
1.5
2.5
3.5
4.5
5.5
6.5
Calibration Curves of TSKgel SuperMultiporeHZ-M, H and N
Columns
Columns: TSKgel SuperMultiporeHZ-N, 3 µm, 4.6 mm ID x 15 cm;
TSKgel SuperMultiporeHZ-M, 4 µm, 4.6 mm ID x 15 cm; TSKgel
SuperMultiporeHZ-H, 6
µm, 4.6 mm ID x 15 cm; Samples: PStQuick
polystyrene standards; Mobile phase: THF; Flow rate: 0.35 mL/min;
Detection: UV@254nm; Temperature: 25˚C
A
50
30
10
-10
10
B
30
20
40
Comparing Semi-Micro and Conventional GPC Columns
Columns: A. Conventional columns, 7.8 mm ID x 30 cm x
4; B. TSKgel SuperMultiporeHZ-N, 4.6 mm ID x 15 cm x 4;
Sample: poly(teramethylene ether glycol); (PTMEG 650), 10 μg/μL;
Mobile phase: THF; Flow rate: A. 1.0 mL/min B. 0.35 mL/min;
Detection: RI; Temperature: 40°C; Injection vol.: A. 50 μL B. 10 μL;
INSTRUMENTS
21
GPC
TSKgel columns and
standards
table 8
Ordering Information
Part #DescriptionParticle Exclusion limitTheoreticalColumn
size (µm)
(polystyrene)
plates/15 cm
dimensions
0021815TSKgel SuperMultiporeHZ-N
3
1.2 x 105 Da
>20,000
4.6 mm ID x 15 cm
0021488TSKgel SuperMultiporeHZ-M
4
2 x 106 Da
>16,000
4.6 mm ID x 15 cm
0021885TSKgel SuperMultiporeHZ-H
6
4 x 107 Da
>11,000
4.6 mm ID x 15 cm
0021886TSKgel SuperMultiporeHZ-H Guardcolumn 6N/AN/A
4.6 mm ID x 2 cm
0021489TSKgel SuperMultiporeHZ-M Guardcolumn 4N/AN/A
4.6 mm ID x 2 cm
0021816TSKgel SuperMultiporeHZ-N Guardcolumn 3N/AN/A
4.6 mm ID x 2 cm
22
GPC
TSKgel columns and
standards
PStQuick GPC Polystyrene Calibration
Standards
PStQuick polystyrene calibration standards contain pre-mixed
quantities of polystyrene polymers in autosampler vials for
the calibration of GPC columns. Addition of solvent is all that
is required for easy preparation and analysis.
12 different kits containing polystyrene polymers of various
molar masses are available. Of the 12 kits, 9 are individual kits,
each containing 3 to 5 polystyrene polymers. The remaining
3 are composite kits containing 2 or 3 of the individual kits.
Figure 25
(3) TSKgel SuperMultiporeHZ-N
12
13
15
14
1 x 108
16 17
18
PStQuick
1 x 107
MP-N
1 x 106
130
(2) TSKgel SuperMultiporeHZ-M
7
6
80
10
11
(1) TSKgel SuperMultiporeHZ-H 3
30
1
-20
8
9
3
(PStQuick MP-H)
5
4
Log molar mass
180
MP-M
5
(PStQuick MP-M)
1.Mw 5480000(Lot No.TS- 30) 6.Mw 706000(Lot No.TS-201)
2.Mw 706000(Lot No.TS-201) 7.Mw 96400(Lot No.TS-144)
3.Mw 96400(Lot No.TS-144) 8.Mw
5970(Lot No.TS-503)
4.Mw 10200(Lot No.TS-508) 9.Mw
474(Lot No.TS-505)
5.Mw
1010(Lot No.TS-507) 10.Mw
370(Lot No.TS-505)
11.Mw
266(Lot No.TS-505)
11
(PStQuick MP-N)
1 x 104
1,000
10
7
9
(PStQuick MP-M)
1 x 105
100
MP-H
2
(PStQuick MP-H)
3
13
5
7
9
11
(PStQuick MP-N)
12.Mw
13.Mw
14.Mw
15.Mw
16.Mw
17.Mw
18.Mw
37900(Lot No.TS-202)
5970(Lot No.TS-503)
682(Lot No.TS-505)
578(Lot No.TS-505)
474(Lot No.TS-505)
370(Lot No.TS-505)
266(Lot No.TS-505)
Chromatograms and calibration curves obtained using the PStQuickMP series
Columns: SuperMultiporeHZ-H, 4.6 mm ID x 15 cm x 2; SuperMultiporeHZ-M, 4.6 mm ID x 15 cm L x 2; SuperMultiporeHZ-N, 4.6 mm ID x
15 cm x 2; Sample: PStQuick MPseries; Mobile phase: THF; Flow rate: 0.35 mL/min; Injection volume: 10 μL; Temperature: 25°C; Detection:
UV@254nm (UV-8020 microcell)
Chromatograms obtained using the PStQuickMP series
Calibration curves obtained using the PStQuickMP series
SuperMultiporeHZ-H, 4.6mm ID x 15cm x 2
SuperMultiporeHZ-M, 4.6mm ID x 15cm x 2
SuperMultiporeHZ-N, 4.6mm ID x 15cm x 2
Mobile phase:
Flow rate:
Temperature:
Detection:
Sample:
THF
0.35mL/min
25°C
UV@254nm(UV-8020 microcell)
PStQuick MPseries 10µL
INSTRUMENTS
23
GPC
TSKgel columns and
standards
table 9
Ordering Information for PStQuick Polystyrene Calibration Standards
To calibrate TSKgel SuperMultiporeHZ Columns
Part #DescriptionRemarksCalibration rangeContentsVials
0021912PStQuick MP-N
For SuperMultiporeHZ-N
5.3 x 102 to 4.4 x 104A-500, A-5000, F-4
60
0021913PStQuick MP-M
For SuperMultiporeHZ-M
5.3 x 102 to 8.0 x 105A-500, A-5000, F-10, F-80
60
0021914PStQuick MP-H
For SuperMultiporeHZ-H
9.5 x 102 to 5.5 x 106A-1000, F-1, F-10, F-80, F-550
60
To calibrate TSKgel H-type Mixed-Bed Columns
0021915PStQuick Kit-L
for H-type – N Grade
5.3 x 102 to 4.2 x 105PStQuick E, F
40**
0021916PStQuick Kit-M
for H-type – M Grade
5.3 x 102 to 2.9 x 106PStQuick C, D
40**
0021917PStQuick Kit-H
for H-type – H Grade
5.3 x 102 to 8.4 x 106PStQuick A, B, C
60*
*20 of each type x 3, **20 of each type x 2
To calibrate TSKgel GPC Columns
0021911PStQuick A
for other GPC columns
2.8 x 103 to 8.4 x 106A-2500, F-2, F-20, F-128, F-850
20
0021910PStQuick B
9.5 x 102 to 5.5 x 106A-1000, F-1, F-10, F-80, F-550
20
0021909PStQuick C
5.3 x 102 to 2.9 x 106A-500, A-5000, F-4, F-40, F-288
20
0021908PStQuick D
2.8 x 103 to 1.3 x 106A-2500, F-2, F-20, F-128
20
0021907PStQuick E
9.5 x 102 to 4.2 x 105A-1000, A-5000, F-4, F-40
20
0021906PStQuick F
5.3 x 102 to 1.9 x 105A-500, A-2500, F-2, F-20
20
24
GPC
TSKgel columns and
standards
TSKgel Polystyrene Calibration Standards
TSKgel polystyrene bulk calibration standards are used to
calibrate size exclusion columns for subsequent analysis
of unknown samples. The standards range from 400 to
21,000,000 Da.
table 10
Ordering Information for TSKgel Polystyrene Calibration Standards
Part #DescriptionWeight
0005202A-300, 400 MW
10 g
0005203A-500, 530 MW
10 g
0005204A-1000, 950 MW
10 g
0005205A-2500, 2800 MW
5g
0005206A-5000, 6200 MW
5g
0005207
F-1, 1.0 x 104 MW
5g
0005208
F-2, 1.7 x 104 MW
5g
0005209
F-4, 4.4 x 104 MW
5g
0005210
F-10, 1.0 x 105 MW
5g
0005211
F-20, 1.9 x 105 MW
5g
0005212
F-40, 4.2 x 105 MW
5g
0005213
F-80, 7.8 x 105 MW
5g
0005214
F-128, 1.3 x 106 MW
1g
0005215
F-288, 2.9 x 106 MW
1g
0005216
F-380, 3.8 x 106 MW
1g
0005217
F-450, 4.5 x 106 MW
1g
0005218
F-550, 5.5 x 106 MW
1g
0005219
F-700, 6.8 x 106 MW
1g
0005220
F-850, 8.4 x 106 MW
1g
0005221
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EcoSEC GPC System
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Applications
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TOSOH BIOSCIENCE
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B15I06A

ecosec GPc sYsTeM - Tosoh bioscience

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