New Pipettors with Active Brake
Transcription
New Pipettors with Active Brake
TN-BF-013 BIO-FLASH Technical Note Upgrade to Pipettors with Active Brake BIO-FLASH® Revision History Revision A Revision B Initial Release of the note Added: - PRE-INSTALLATION PAWL INSPECTION PROCEDURE - WASHER INSTALLATION PROCEDURE New Pipettors with Active Brake - UPGRADE KIT INSTRUCTIONS For technical service use only. A redesigned Pipettor brake mechanism has been made available in order to overcome the limitations of the Haydon nut in certain environments. The Haydon nut back-off procedure solves any problems related to the stalls of the arms. This UPGRADE is not mandatory but highly recommended Warning: It is mandatory that all materials be read and understood completely before starting any upgrade. Page 1 of 95 ECR# 427710 TN-BF-013 BIO-FLASH Technical Note Upgrade to Pipettors with Active Brake CONTENTS of the UPGRADE KIT The kit comes in a box with the following label In the box the following items will be found Accessories box Sample Pipettor Reagent Pipettor Page 2 of 95 ECR# 427710 TN-BF-013 BIO-FLASH Technical Note Upgrade to Pipettors with Active Brake In the accessories box you will find 6 different plastic bags that are described below with the part numbers are: BAG #1 Tie wrap mount. Plastic stickers for cable routing into the unit BAG #2 P-clamp and associated screws Page 3 of 95 ECR# 427710 TN-BF-013 BIO-FLASH Technical Note Upgrade to Pipettors with Active Brake BAG #3 Reagent Carousel Cable Holder Bracket (M/C: 011-00604ADF Rev D) Metal bracket and spare plastic clamp. Page 4 of 95 ECR# 427710 TN-BF-013 BIO-FLASH Technical Note Upgrade to Pipettors with Active Brake BAG #4 Assorted plastic ties (short and long) BAG #5 Sample Pipettor Override Switch (0009803290) Reagent Pipettor Override Switch (0009803291) Jumper cable (0009803289) Active brake power cable (0009803292) Cable set to be used. BAG #6 Page 5 of 95 ECR# 427710 TN-BF-013 BIO-FLASH Technical Note Upgrade to Pipettors with Active Brake Sample Pipettor Active brake PCB (0009803288) (comes with Sample/Reagent Pipettor Active Brake PCB support installed) Reagent Pipettor Active brake PCB (0009803287) (comes with Sample/Reagent Pipettor Active Brake PCB support installed) REQUIRED TOOLS Allen keys (M5, M4 and M3). Torque tools (adjustments for 23 in-lbs and 100 in-lbs are needed) Alcohol Light Tweezers Cutter Pipettor Arm Wrench (0009803302) Pipettor Arm Tool (0009803304) The unit must be accessed from the front and back, so make sure the instrument is on top of a bench with 360º accessibility. Page 6 of 95 ECR# 427710 TN-BF-013 BIO-FLASH Technical Note Upgrade to Pipettors with Active Brake PIPETTORS UNPACKING Pipettors descriptions and part numbers are: SAMPLE PIPETTOR ASSY (W/BRAKE) (0009803286) REAGENT PIPETTOR ASSY (W/BRAKE) (0009803285) Unpacking and handling instructions: Place the Pipettor on the packaging top foam until needed. See picture below. Page 7 of 95 ECR# 427710 TN-BF-013 BIO-FLASH Technical Note Upgrade to Pipettors with Active Brake Caution: GOOD SAFETY PRACTICES • • • • Wear lab coat and gloves during the upgrade. Clean the instrument surface before working on the unit with a 10% bleach solution. Before starting the UPGRADE make sure you have an area where you will be able to place all the components removed from the system. Before starting the installation make sure you understand the active brake blocking mechanism and the way to override it manually. You must to override the Pipettors manually during the installation to see if there is any cable interference with other components in the system. MANUAL OVERRIDE of the BLOCKING MECHANISM With a finger touching the active brake pawl and moving it clockwise you are able to unblock the Z movement of the arm. Familiarize yourself with it before starting. You can manually move the Pipettor up at any time. However, once in the up position the only way to make it go down is overriding the active brake solenoid manually as previously described. Familiarize yourself with the manual override of the blocking mechanism as shown below Override direction Page 8 of 95 ECR# 427710 TN-BF-013 BIO-FLASH Technical Note Upgrade to Pipettors with Active Brake Pawl at the blocking position When moving the base of the pawl in the direction of the yellow arrow, the tip of the pawl will move in the direction of the green arrow. Practice the movement with the Pipettors out of the unit in order to familiarize yourself with the override action. Page 9 of 95 ECR# 427710 TN-BF-013 BIO-FLASH Technical Note Upgrade to Pipettors with Active Brake PRE-INSTALLATION PAWL INSPECTION PROCEDURE After the releasing of the kits it was detected in manufacturing a potential problem due to tolerance of the pawl mounting screws. The following procedure describes how to inspect the pawls in order to detect out of tolerance screws that could compromise the functioning of the active brake mechanism or the Pipettor Z movement. 2 3 5 4 1 Check each pawl for the M2.5 mounting screws extending above the mounting plate of the solenoid. If they extend above the plate then removal of the Pawl and insertion of M2.5 flat washer between the Brake Pawl and the mounting plate of the solenoid has to be done. The image above shows already the spacing M2.5 washers in place, this is not the case in the released upgrade kits. The numbers listed below describe the numbered boxes in the drawing above. 1. M2.5 Flat washer installed 2. M2.5 Lock washer installed 3. Solenoid Mounting Plate Page 10 of 95 ECR# 427710 TN-BF-013 BIO-FLASH Technical Note Upgrade to Pipettors with Active Brake 4. Active Brake Pawl 5. M2.5 x5 SHCS stainless steel NOTE: Contact the instrument provider to supply the M2.5 washers to do this installation. Detail of the area to inspect The yellow squared area has to be free of any screw tip. In the picture can be seen only the bearings that allow a smooth rotation of the pawl. Because this picture corresponds to a pawl with washers in place, the screws do not stand above the pawl support and no screw end appears in the yellow squared area. Note- In the case you need to correct the pawls installation, with the addition of the lock washer there is no need to apply any thread locker to the mounting screw. The next section is a step by step guide on how to install the washers in case they are necessary. Page 11 of 95 ECR# 427710 TN-BF-013 BIO-FLASH Technical Note Upgrade to Pipettors with Active Brake Both Pipettors have to be inspected in order to detect if the washer modification of the pawls is necessary to be carried out. Not doing so will compromise the performance of the Pipettors and might require a further intervention upon the unit to correct this. NOTE: Contact the instrument provider to supply the M2.5 washers to do this installation. Page 12 of 95 ECR# 427710 TN-BF-013 BIO-FLASH Technical Note Upgrade to Pipettors with Active Brake WASHER INSTALLATION PROCEDURE Place robot on an appropriate surface Loosen all three screws until only a couple threads are engaged. Separation between Pawl and solenoid mounting plate (first screw removed) You should be able to see approximately 3 mm space between the Pawl and the Solenoid Mounting Plate. Page 13 of 95 ECR# 427710 TN-BF-013 BIO-FLASH Technical Note Upgrade to Pipettors with Active Brake Remove one screw and place a 2.5 mm lock washer on it NOTE: Contact the instrument provider to supply the M2.5 washers to do this installation. This will be done for all three screws used to attach the Pawl to the Solenoid Mounting Plate Place the M2.5 flat washer between the Pawl and the Solenoid mounting plate and insert the M2.5 screw with lock washer. Do not fully seat the mounting screw. Page 14 of 95 ECR# 427710 TN-BF-013 BIO-FLASH Technical Note Upgrade to Pipettors with Active Brake Remove the next screw- and install M2.5 lock washer- Place the M2.5 flat washer between the Pawl and the Solenoid mounting plate. Install the M2.5 screw with lock washer –repeat the steps for each screw. Seat / tighten all three Pawl mounting screws. Confirm all the M2.5 Flat washers are in place between the Pawl and the Solenoid Mounting Plate and each mounting screw has a M2.5 Lock washer between the head of the screw and the Pawl. Page 15 of 95 ECR# 427710 TN-BF-013 BIO-FLASH Technical Note Upgrade to Pipettors with Active Brake Rotate the pawl and release-confirm no binding or stopping. Confirm the tip of the Brake Pawl is seated in the groove of the Pulley. Page 16 of 95 ECR# 427710 TN-BF-013 BIO-FLASH Technical Note Upgrade to Pipettors with Active Brake UPGRADE PROCEDURE 1. Perform an Empty Lines. 2. Remove all reagent cartridges and place them in a 2 – 6°C refrigerator. 3. Remove the reagent basket. 4. Power-off the instrument and remove power cord. 5. Remove Sample arm cover and Reagent arm cover. 6. Remove instrument covers (Refer to Service Manual Chapter 7). NOTE: Keep the covers in a secure place to avoid damaging them. The area surrounding the instrument should be as clear as possible to avoid problems while working on the instrument. 7. Remove the Pipettor probes and keep them in a safe place, this will avoid any damage of the probes during the upgrade process (refer to Service Manual Chapters 9-1 (Reagent) and 10-1 (Sample)). NOTE: If you did not perform an Empty Lines, then disconnecting the tubes will spill system rinse upon the level sense board or the instrument. Remove the lines carefully with the aid of absorbent paper or fabric. Before disconnecting the tubing, place the probes above the corresponding wash towers in order to collect any drops from them. Warning: DO NOT MIX PROBES!!!! Clearly identify them in order to place them back to their original Pipettor. See example below of labeling to differentiate them (a simple color tape is used in the example). SAMPLE PROBE REAGENT PROBE Page 17 of 95 ECR# 427710 TN-BF-013 BIO-FLASH Technical Note Upgrade to Pipettors with Active Brake 8. Remove Sample Module (see Service Manual Chapter 12-1). 9. Remove Reagent Module (refer to Service Manual Chapter 11-1). 10. Loosen the electronics enclosure screws at the rear panel and slide it back to ease the process. Disconnect any cable that reaches the backplane board and front interconnect board. NOTE: With a paper cloth dry any condensation remaining into the reagent bowl in order to avoid any spillage during the removal process. Keep the carousel in a safe place and handle it with care. ATENTION: Make sure the 3 thermal isolators below the reagent carousel are present (on the base or in the reagent carousel. Keep them in a safe place until installing the Reagent Carousel Assy back into the unit. The absence of a thermal isolator compromises the instrument performance in terms of the cooling capability of the reagent carousel. 11. Remove Sample Pipettor (see Service Manual Chapter 10-2) NOTE: Pay attention when disconnecting the Sample Pipettor cables and cables routed through the clamp before proceeding with the removal of the module. When detaching it from the Incubator Distributor, a black plastic spacer will be seen, this piece must be reused when installing the new sample Pipettor. Picture of the plastic spacer is shown below. Page 18 of 95 ECR# 427710 TN-BF-013 BIO-FLASH Technical Note Upgrade to Pipettors with Active Brake Page 19 of 95 ECR# 427710 TN-BF-013 BIO-FLASH Technical Note Upgrade to Pipettors with Active Brake Instrument configuration must be as shown below before placing the new Pipettors into the instrument. The thermal isolators must be in the yellow areas (in the figure only one is left, the other two are in the foam of the reagent carousel). Inspect the instrument casting below the reagent carousel and dry any water that could have accumulated in the casting compartments. Page 20 of 95 ECR# 427710 TN-BF-013 BIO-FLASH Technical Note Upgrade to Pipettors with Active Brake 12.Install the Sample Pipettor with active brake. NOTE: Do not forget the Sample Pipettor spacer! When installing the Sample Pipettor in the unit, do not pinch any cables and route them to the back, exiting from the rear side of the Pipettor to allow a further routing step. Refer to the figure below: Page 21 of 95 ECR# 427710 TN-BF-013 BIO-FLASH Technical Note Upgrade to Pipettors with Active Brake Picture on the left shows the pre-placement of the Pipettor. In this phase the screws are in place but not completely tightened. When tightening the screws, make sure the Pipettor is slightly forced in the direction shown with the yellow arrow. Verify that there is no cable interference in the far up and low positions of the Pipettor. Override the Pipettor manually to bring it down and check for interference. Make sure the cables are not rubbing against any part. UP DOWN MIDDLE NOTE: When moving the Pipettor up manually to the far up position the connector lock of the flat cable will interfere against the sample wash tower. This is as designed, the home position of the Sample Pipettor Z movement happens before reaching this point. 13.Once the Sample Pipettor is fixed in place, guide the sample pump tubing that connects to the probe, through the internal part of the shaft. 14.Install the SAMPLE PIPETTOR ACTIVE BRAKE PCB (0009803288) (assembled with the Sample Pipettor Active Brake PCB support in BAG#6) as shown in the yellow circle. Warning: In this step the PCB support has to remain unfastened. The PCB support is not tightened until Step 36!! Page 22 of 95 ECR# 427710 TN-BF-013 BIO-FLASH Technical Note Upgrade to Pipettors with Active Brake Identify the board with Sample Brake label Placement detail of the board and support 15.Route the Sample Pipettor cables through the white P clamps as shown in the figure below Page 23 of 95 ECR# 427710 TN-BF-013 BIO-FLASH Technical Note Upgrade to Pipettors with Active Brake 4 cables from the Pipettor Routing is complete 16.Attach the square tie wrap mount as seen in the picture below (BAG#1). The placement is shown in the figure below use, as a reference, the hole in the base casting in the vicinity of the cables. Prior to removing the adhesive protection paper, clean the surface with alcohol where the tie wrap will be mounted. Page 24 of 95 ECR# 427710 TN-BF-013 BIO-FLASH Technical Note Upgrade to Pipettors with Active Brake Overview of the placement Detail of the placement 17.Place a short tie wrap (BAG #4) to group the cables as shown below. Do not completely tighten this tie wrap; some room has to be left for additional cables. 18.Connecting the cables. Motor cable labeled P601 has to be connected to J601 in the active brake board Solenoid cable P604 has to be connected to J604 in the active brake board Reconnect to Interconnect PCB #2 the two sensor cables labeled P222 and P221. Page 25 of 95 ECR# 427710 TN-BF-013 BIO-FLASH Technical Note Upgrade to Pipettors with Active Brake Interconnect PCB #2 P222 P221 P601 P604 19. Sample Pipettor flat cable installation instructions. a. Place the spare cable clamp (Bag #3) as shown in the picture below (label end is the reference point to align the clamp): Page 26 of 95 ECR# 427710 TN-BF-013 BIO-FLASH Technical Note Upgrade to Pipettors with Active Brake b. Fold the cable as shown (make sure the fold remains). This step will allow a proper routing of the cables out of the plastic clamp. NOTE: After the fold is done, remove the clamp. Page 27 of 95 ECR# 427710 TN-BF-013 BIO-FLASH Technical Note Upgrade to Pipettors with Active Brake 20. Install the Sample/Reagent Pipettor override switches. There are two holes in the left side of the unit where the peristaltic pump is located (see detail below). Page 28 of 95 ECR# 427710 TN-BF-013 BIO-FLASH Technical Note Upgrade to Pipettors with Active Brake The Sample Pipettor override switch cabling has to be installed in the hole closest to the front part of the instrument. The cable is in BAG#5 and its label is P603 (is the longer one of the two override switch cables). Discard the flat washer. Locking Washer Nut The locking washer and nut are installed in the outer part of the unit, as seen below. Page 29 of 95 ECR# 427710 TN-BF-013 BIO-FLASH Technical Note Upgrade to Pipettors with Active Brake Tighten it. Page 30 of 95 ECR# 427710 TN-BF-013 BIO-FLASH Technical Note Upgrade to Pipettors with Active Brake Proceed with the routing of the cable. Under the motor Page 31 of 95 ECR# 427710 TN-BF-013 BIO-FLASH Technical Note Upgrade to Pipettors with Active Brake Around the power supply Open the fluidics drawer (if needed) to route the cable behind the incubator. Page 32 of 95 ECR# 427710 TN-BF-013 BIO-FLASH Technical Note Upgrade to Pipettors with Active Brake Leave the cable like shown in the figure (for now) Proceed now with the Reagent Pipettor override switch following the same steps done for the Sample Pipettor Override Switch (this time use the cable labeled P703 from BAG#5): - Discard the flat washer. - Install the washer and the nut in the outer part of the unit. - Tighten it. - Proceed with the routing of the cable, following the same path as the sample. The Reagent Pipettor override switch is installed in the other hole, furthest from the front of the unit (as shown in figure below). Page 33 of 95 ECR# 427710 TN-BF-013 BIO-FLASH Technical Note Upgrade to Pipettors with Active Brake Place to install the Reagent Pipettor Override Switch 21. Remove the Luminometer black cover to ease the installation of the power supply cable, the black and red twisted one (0009803292) that splits into two and labeled P605 and P705 (It can be found into BAG#5). Page 34 of 95 ECR# 427710 TN-BF-013 BIO-FLASH Technical Note Upgrade to Pipettors with Active Brake Remove the plastic protection of the power supply terminals and install the power supply cable as is shown in the picture below. RED BLACK Be sure that both cables are placed as shown. Page 35 of 95 ECR# 427710 TN-BF-013 BIO-FLASH Technical Note Upgrade to Pipettors with Active Brake Tighten the screws and place the terminals plastic cover back. Page 36 of 95 ECR# 427710 TN-BF-013 BIO-FLASH Technical Note Upgrade to Pipettors with Active Brake Route the cable along with the override switched cables. Replace the Luminometer black cover. 22. Route the 3 cables behind the aluminum post. 23. Install the Reagent Pipettor Active Brake PCB (0009803287) as shown below (assembled with the Reagent Pipettor Active Brake PCB support in BAG#6). Page 37 of 95 ECR# 427710 TN-BF-013 BIO-FLASH Technical Note Upgrade to Pipettors with Active Brake BOARD - Page 38 of 95 ECR# 427710 TN-BF-013 BIO-FLASH Technical Note Upgrade to Pipettors with Active Brake Tighten the board in place through the upper support screw (see detail below). 24. Clean the area that the plastic cable holder will be installed to, with alcohol. Wait for the alcohol to dry (~1 minute) and install the plastic cable holder from BAG#1 (see picture below). Page 39 of 95 ECR# 427710 TN-BF-013 BIO-FLASH Technical Note Upgrade to Pipettors with Active Brake Clean with alcohol Place the plastic holder 25. Using a long plastic tie (BAG #4) tighten the cables as is shown in the picture below (behind the post). The tie does not have to grab the tubes that run close to the aluminum Page 40 of 95 ECR# 427710 TN-BF-013 BIO-FLASH Technical Note Upgrade to Pipettors with Active Brake post. Tighten the tie and cut the excess. Make sure the plastic head of the tie wrap stays as close to the tubes as possible. See yellow arrow indicating proper placement. 26.Route only the Power Supply Cable (P605) and the Sample Pipettor Override Switch (P603) through the plastic tie in the casting. As shown in the picture below. Page 41 of 95 ECR# 427710 TN-BF-013 BIO-FLASH Technical Note Upgrade to Pipettors with Active Brake Arrange the cables through the plastic tie wrap and P-clamp. Tidy them up and completely close the tie wrap. Page 42 of 95 ECR# 427710 TN-BF-013 BIO-FLASH Technical Note Upgrade to Pipettors with Active Brake Page 43 of 95 ECR# 427710 TN-BF-013 BIO-FLASH Technical Note Upgrade to Pipettors with Active Brake Connect the two new cables to the Sample Pipettor Active Brake PCB (P603 to J603 and P605 to J605). P603 J603 P605 J605 NOTE: It may be necessary to tie the two cables out of the way in order to prevent contact with the sample pump tubing. 27. You can connect the other two cables, labeled P703 and P705, to the corresponding connectors in the Reagent Pipettor Active Brake PCB. Make sure they go through the plastic clamp as shown in the figure below. Page 44 of 95 ECR# 427710 TN-BF-013 BIO-FLASH Technical Note Upgrade to Pipettors with Active Brake P703 P705 28.Modification of the Reagent Carousel In this section we are going to use the new Reagent Pipettor with Active Brake (0009803286) and a modified bracket (BAG #3) that will allow the proper guidance of the flat flexible cables. Remove the old Pipettor from the reagent carousel (see Service Manual Chapter 9-1). Remove the bracket piece from the reagent carousel shown in the figure below: Page 45 of 95 ECR# 427710 TN-BF-013 BIO-FLASH Technical Note Upgrade to Pipettors with Active Brake OLD BRACKET Cut the plastic ties holding the cabling on the right side. Remove the 3 plastic screws holding the bracket piece. Install the new supplied bracket (BAG #3). Reinstall the plastic ties accordingly to hold the cables in place. CABLE HOLDER NEW BRACKET Make sure the tie wrap is oriented correctly, otherwise will interfere with UP/DOWN movement of the Pipettors. Page 46 of 95 ECR# 427710 TN-BF-013 BIO-FLASH Technical Note Upgrade to Pipettors with Active Brake INCORRECT CORRECT Close the plastic clamp in the new supplied bracket as shown in the picture below. INCORRECT Open Clamp Page 47 of 95 CORRECT Closed Clamp ECR# 427710 TN-BF-013 BIO-FLASH Technical Note Upgrade to Pipettors with Active Brake Write the original number of the reagent carousel upon the clean curved surface of the bracket with a permanent marker (see example below). 29.Attach the Reagent Pipettor with Active Brake to the Reagent Carousel Page 48 of 95 ECR# 427710 TN-BF-013 BIO-FLASH Technical Note Upgrade to Pipettors with Active Brake Page 49 of 95 ECR# 427710 TN-BF-013 BIO-FLASH Technical Note Upgrade to Pipettors with Active Brake Verify once more the plastic clamp, it should be closed. Incorrect (clamp open) Correct (clamp close) Pre-install a tie wrap (long) through the 2 holes of flat cable holding bracket as shown below Page 50 of 95 ECR# 427710 TN-BF-013 BIO-FLASH Technical Note Upgrade to Pipettors with Active Brake Page 51 of 95 ECR# 427710 TN-BF-013 BIO-FLASH Technical Note Upgrade to Pipettors with Active Brake 30.Now route the flat cable labeled P6 as the shown below. The cable is going behind the linear bearing guide to the heat sink area. Make sure that there is NO TWIST in the cable. The lateral view is shown for reference. Page 52 of 95 ECR# 427710 TN-BF-013 BIO-FLASH Technical Note Upgrade to Pipettors with Active Brake 31.Proceed to attach the flat cable at the P clamp provided in BAG #2, as shown below. Place the cable through the P-clamp prior to its attachment against the heat sink. Before After 32.Ensure the Reagent Pipettor moves freely up and down with no interference. 33.Placement of the flat cable into the plastic clip. Move the Pipettor highest Z position. Page 53 of 95 ECR# 427710 TN-BF-013 BIO-FLASH Technical Note Upgrade to Pipettors with Active Brake Place the cable in the clamp. The clamp position for the cable is given by the end of the cable label, as shown in the picture below. Preform the cable with a 90º fold to facilitate its routing after installing the reagent module. 34. If you have not done so previously place the thermal isolators back on the casting posts. 35.At this point in time and before placing the reagent carousel back to the instrument proceed with the following cable connection. Take cable labeled as P701/P5 from BAG #5 and connect it from the Reagent Brake PCB board to the backplane board J5 connector. See images below as a reference. Page 54 of 95 ECR# 427710 TN-BF-013 BIO-FLASH Technical Note Upgrade to Pipettors with Active Brake NOTE: The guidance of this cable will require cutting the below indicated plastic tie. To install a new plastic tie, remove the electronics enclosure cover and remove the boards inside. After tightening the tie place the boards back into the electronics enclosure. ELECTRONICS ENCLOSURE COVER Cut existing tie and place a new one. Detail on how the plastic tie has to be in order to avoid any interference with moving cables. Page 55 of 95 ECR# 427710 TN-BF-013 BIO-FLASH Technical Note Upgrade to Pipettors with Active Brake The closer the plastic tie head is to the metal enclosure the better. Tidy up the cabling of the reagent carousel to avoid interferences with other modules when placing it back to the instrument. Keep the Pipettor in the upper position. Page 56 of 95 ECR# 427710 TN-BF-013 BIO-FLASH Technical Note Upgrade to Pipettors with Active Brake 36. Place the Reagent Module back upon the casting. Make sure nothing is pinched and drainage tubes fall into the corresponding area on the casting. Page 57 of 95 ECR# 427710 TN-BF-013 BIO-FLASH Technical Note Upgrade to Pipettors with Active Brake Make sure the fluid connection tubing inside the Reagent Pipettor shaft goes through the 2 P clamps on the casting (see picture below). To Reagent Pipettor Shaft From Reagent Valve NOTE: Verify nothing is being pinched before tightening the Reagent Carousel screws. Now connect the solenoid cable (P704) and the interconnect cable (P702) to the Reagent Pipettor Active Brake PCB, as it is shown in the next figure. It is difficult because it must reach the board from below. Place the cables at the bottom of the casting and route them to the board. They only have one fixing point, the plastic clip previously installed. Here you have a sample of how it must be layed out. Page 58 of 95 ECR# 427710 TN-BF-013 BIO-FLASH Technical Note Upgrade to Pipettors with Active Brake After clipping the cables move the bundle upwards Make sure the cables are at the same level as the plastic clip; otherwise they could be pinched by the backplane board when putting the electronics enclosure back to the system. The recommendation is to pull them upwards to avoid any pinching, see next figure for pinching indication. CHECK ONLY DO NOT PLACE THE ENCLOSURE BACK Potential pinching of the cables with the backplane board. Page 59 of 95 ECR# 427710 TN-BF-013 BIO-FLASH Technical Note Upgrade to Pipettors with Active Brake 37. Clip the Reagent and Sample flat cables in the clip mount of the bracket. To facilitate this operation, is strongly recommended to do the following: i) Remove Reagent Probe Wash Tower and place it over the incubator as shown: ii) Lift Sample Pipettor totally up iii) Lower Reagent Pipettor deep all the way. Open the clip to allow the Reagent cable to be free. The first cable into this clip is the Sample Pipettor cable, this cable comes with a folded part (previously folded) that will indicate is positioning in the clip. Page 60 of 95 ECR# 427710 TN-BF-013 BIO-FLASH Technical Note Upgrade to Pipettors with Active Brake We have to manage to place the Sample Pipettors first in the clip making sure that it exits towards the front of the instrument. Once this is done we should be able to see the Sample Pipettor cable coming out towards the front part of the instrument as the picture below Bracket From Sample Pipettor Instrument front Page 61 of 95 ECR# 427710 TN-BF-013 BIO-FLASH Technical Note Upgrade to Pipettors with Active Brake For the Reagent Pipettor Cable the routing is as shown: Instrument back From Reagent pipettor Bracket Page 62 of 95 ECR# 427710 TN-BF-013 BIO-FLASH Technical Note Upgrade to Pipettors with Active Brake Both together should look like the drawing below. Notice the presence of a plastic tie that keeps the cables together when exiting laterally. Instrument back From Reagent pipettor Plastic clip From Sample pipettor Bracket Plastic tie Instrument front Clip them together in the clip installed on the bracket. The cables must run parallel exiting the bracket, as shown in the previous drawing. Page 63 of 95 ECR# 427710 TN-BF-013 BIO-FLASH Technical Note Upgrade to Pipettors with Active Brake Use the pre-installed plastic tie wrap from step 24 to tie both flat cables against the bracket in order to avoid blocking the encoder wheel. Confirm that flat cables do not interfere with the reagent Pipettor encoder (see below). Flat Cables Bracket Encoder The Sample Pipettor cable (P216) that goes to the front should be twisted to the front and routed Page 64 of 95 ECR# 427710 TN-BF-013 BIO-FLASH Technical Note Upgrade to Pipettors with Active Brake behind the Sample Pipettor Active Brake PCB. Prior to tightening the support in place make sure the flat cable is as close as possible to the lower part of the casting to avoid any pinching. Example shown below. As low as possible Now fasten the PCB support upper screw. Page 65 of 95 ECR# 427710 TN-BF-013 BIO-FLASH Technical Note Upgrade to Pipettors with Active Brake Page 66 of 95 ECR# 427710 TN-BF-013 BIO-FLASH Technical Note Upgrade to Pipettors with Active Brake 38.Connect the Sample Pipettor flat cable (P216) to the front board (J216) as shown in the picture 39.Connect the Reagent Pipettor flat cable (P6) to Backplane PCB (J6). 40.Connect the jumper cable (BAG#5): 1st end - connector P602 to J602 on the Sample Pipettor Active Brake PCB. 2nd end - connector P223 to J223 on the Interconnect PCB#2 (under reagent carousel) 41. Reconnect all cables to Backplane PCB and bring the fluidics drawer and the electronics enclosure back to the closed position. In the process of doing this make sure to not pinching any cable. 42. Replace reagent wash tower (with corresponding tubes) if it was removed in step 33. Page 67 of 95 ECR# 427710 TN-BF-013 BIO-FLASH Technical Note Upgrade to Pipettors with Active Brake Page 68 of 95 ECR# 427710 TN-BF-013 BIO-FLASH Technical Note Upgrade to Pipettors with Active Brake 43. Remove the Reagent Tower Cap and align the Wash Tower by hand. The long axis of the inner oval Wash Cup should point directly at the Reagent Arm Shaft (see picture below). Replace the Reagent Wash Tower Cap. Perform the same type of alignment if the Sample Wash Tower has been taken out or moved. 44.Replace Sample Module (refer to Service Manual Chapter 12-1). 45. Replace Sample Probe and Reagent Probes (refer to Service Manual Chapter 10-2 & 9-1). 46.The instrument should be turned on in order to be able to move the Pipettor down using the override switches. Check the override switches moving the arms up by hand and then down with the help of the override switches to make sure the override mechanism works. WARNING: If any of the override switches do not seem to release the corresponding Pipettor, it could be as a consequence of the Pipettor being at its uppermost position. To unlock the Pipettor press its corresponding switch while pulling the Pipettor shaft up and then immediately down. NOTE: If the instrument is powered off, the override switches do not work. Page 69 of 95 ECR# 427710 TN-BF-013 BIO-FLASH Technical Note Upgrade to Pipettors with Active Brake Page 70 of 95 ECR# 427710 TN-BF-013 BIO-FLASH Technical Note Upgrade to Pipettors with Active Brake REAGENT PIPETTOR ALIGMENT 47.Attach the PIPETTOR ARM TOOL (PN 0009803304) as shown in the figure. The ground connection terminal should be taken out in order to accommodate the tool (sometimes cutting the plastic tie allows proper placement of the tool). 48.Loosen the M3 and M5 screws on the side of the Reagent Pipettor Arm just enough to allow the arm to rotate stiffly on the Pipettor Shaft. This should allow the arm to move freely in the radial direction as shown in the pictures below. The M5 screw may limit this movement if not loose enough. M3 Page 71 of 95 M5 ECR# 427710 TN-BF-013 BIO-FLASH Technical Note Upgrade to Pipettors with Active Brake 49.By hand rotate the arm until the probe is above Reagent Carousel position B. D C B A 50. Press the Reagent override switch, and lower the probe down into the hole until the pipette nut base is inside the groove of the Reagent Carousel cover. Page 72 of 95 ECR# 427710 TN-BF-013 BIO-FLASH Technical Note Upgrade to Pipettors with Active Brake B Page 73 of 95 ECR# 427710 TN-BF-013 BIO-FLASH Technical Note Upgrade to Pipettors with Active Brake 51.Adjust the length of the arm manually so that the pipette nut base is centered in the groove and does not hit either the wall of the groove. 52.Tighten the M3 screw on the side of the arm to 23 in-lbs. Page 74 of 95 ECR# 427710 TN-BF-013 BIO-FLASH Technical Note Upgrade to Pipettors with Active Brake 53.Turn the hex-head jack screw to set the mechanical spacing of the arm to the extension gap. Hex head screw 54. By hand bring the Pipettor up as high as it will go in the Z axis. Page 75 of 95 ECR# 427710 TN-BF-013 BIO-FLASH Technical Note Upgrade to Pipettors with Active Brake 55.Twist the Pipettor shaft counter clockwise until the Rotational Stop is hit. Page 76 of 95 ECR# 427710 TN-BF-013 BIO-FLASH Technical Note Upgrade to Pipettors with Active Brake 56.While twisting the shaft against the stop, rotate the Arm on the shaft so that the probe is in the groove of the Reagent Carousel cover, 5-8 mm from the end of the groove past the Reagent Carousel position A. See the picture sequence below. Page 77 of 95 ECR# 427710 TN-BF-013 BIO-FLASH Technical Note Upgrade to Pipettors with Active Brake A Place the probe not over A, just beyond it. Page 78 of 95 ECR# 427710 TN-BF-013 BIO-FLASH Technical Note Upgrade to Pipettors with Active Brake 5-8 mm 57.Ensure that the Pipettor Arm is fully seated on the shaft and snug the M5 screw. Page 79 of 95 ECR# 427710 TN-BF-013 BIO-FLASH Technical Note Upgrade to Pipettors with Active Brake 58.Check that the probe is still 5-8 mm from the end of the groove. If not, loosen the M5 screw and repeat the process from previous two steps. 59.Tighten the M5 screw to 100 in-lbs. 60.Remove the PIPETTOR ARM TOOL and replace the ground terminal back to the original position. If the plastic tie was removed reinstall a new tie. 61. Install the Pipettor cover onto the Reagent Arm. SAMPLE PIPETTOR ALIGMENT 62.Remove the sample basket from the instrument. Page 80 of 95 ECR# 427710 TN-BF-013 BIO-FLASH Technical Note Upgrade to Pipettors with Active Brake 63.Attach the PIPETTOR ARM TOOL as shown in the figure. The ground connection terminal should be taken out in order to accommodate the tool. Page 81 of 95 ECR# 427710 TN-BF-013 BIO-FLASH Technical Note Upgrade to Pipettors with Active Brake 64.Loosen the M3 and M5 screws on the side of the Sample Pipettor Arm just enough to allow the arm to rotate stiffly on the Pipettor shaft. This should allow the arm to move freely in the radial direction as shown in the pictures below (M5 screw may limit this movement if it is not loose enough). M3 M5 65.By hand rotate the arm until the probe is above Sample R Reagent D position. D Page 82 of 95 C B A ECR# 427710 TN-BF-013 BIO-FLASH Technical Note Upgrade to Pipettors with Active Brake Page 83 of 95 ECR# 427710 TN-BF-013 BIO-FLASH Technical Note Upgrade to Pipettors with Active Brake 66.Press the override switch, and lower the probe down into the hole until the pipette nut base is inside the groove of the Reagent Carousel cover. D 67.Adjust the length of the arm manually so that the pipette nut base is centered in the groove and does not hit either the wall of the groove. Page 84 of 95 ECR# 427710 TN-BF-013 BIO-FLASH Technical Note Upgrade to Pipettors with Active Brake 68.Tighten the M3 screw on the side of the arm to 23 in-lbs. 69.Turn the hex-head jack screw to set the mechanical spacing of the arm to the extension gap. Hex head screw 70.Remove the tool and check that the adjustment also is good for the Sample Carousel groove. Fine tune it if needed, loosing M3 and adjusting it again. 71.Reinstall the PIPETTOR ARM TOOL. Page 85 of 95 ECR# 427710 TN-BF-013 BIO-FLASH Technical Note Upgrade to Pipettors with Active Brake 72.By hand bring the Pipettor up as high as it will go in the Z axis. 73. Twist the Pipettor shaft counter clockwise until the Rotational Stop is hit. Page 86 of 95 ECR# 427710 TN-BF-013 BIO-FLASH Technical Note Upgrade to Pipettors with Active Brake 74.While twisting the shaft against the stop, rotate the arm on the shaft so that the probe just touches the Reagent Cover on the far side of the Sample R Reagent D position. See pictures below. Page 87 of 95 ECR# 427710 TN-BF-013 BIO-FLASH Technical Note Upgrade to Pipettors with Active Brake Page 88 of 95 ECR# 427710 TN-BF-013 BIO-FLASH Technical Note Upgrade to Pipettors with Active Brake 75.Ensure that the Pipettor Arm is fully seated on the shaft and snug the M5 screw. 76.Check that the probe still just touches the Reagent Cover. If not, loosen the M5 screw and repeat from two steps before. 77.Tighten the M5 screw to 100 in-lbs. 78.Remove the PIPETTOR ARM TOOL and replace the ground terminal back to the original position. Page 89 of 95 ECR# 427710 TN-BF-013 BIO-FLASH Technical Note Upgrade to Pipettors with Active Brake Page 90 of 95 ECR# 427710 TN-BF-013 BIO-FLASH Technical Note Upgrade to Pipettors with Active Brake 79.Replace the sample basket 80.Install the Pipettor cover onto the Sample Arm. ALIGMENTS CHECKING 81.Through the Field Service Software verify/set the following alignments Sample Pipettor Reagent Pipettor Sample Pipettor to Incubator/Distributor. ATTENTION: Do not reinstall the incubator covers because they must be removed in the next step. Reagent Pipettor to Incubator/Distributor Sample Pipettor to Sample Carousel. Sample Pipettor to Reagent Carousel. Reagent Pipettor to Reagent Carousel Page 91 of 95 ECR# 427710 TN-BF-013 BIO-FLASH Technical Note Upgrade to Pipettors with Active Brake CYCLE TESTING THE PIPETTORS 82.Within the Field Service Software go into the Cycle Tests wizard. NOTE: The purpose of running the Z cycle tests is to detect if there is any rubbing of cables against any piece of hardware. Inspect the area where the flex cables come together and the surrounding area to make sure that during the Z motion there is no friction or rubbing against any other part. Make sure that the tubing is able to move freely and it is not touched by cables, if it is rearrange the cables. ATENTION: Home Sample Z, Sample R, Reagent Z and Reagent R between these two tests and at the end of the testing. Homing is available on the left hand side of the wizard screen. Homing area of the Pipettor Cycle Test Wizard Page 92 of 95 ECR# 427710 TN-BF-013 BIO-FLASH Technical Note Upgrade to Pipettors with Active Brake Warning: Always home the Z and R movements before running the cycle test and before exiting the Wizard. Click on Pipettor Cycle Tests Page 93 of 95 ECR# 427710 TN-BF-013 BIO-FLASH Technical Note Upgrade to Pipettors with Active Brake Sample Pipettor Z movement (100 cycles). Once selected and set, click on Run Test to start the testing Page 94 of 95 ECR# 427710 TN-BF-013 BIO-FLASH Technical Note Upgrade to Pipettors with Active Brake Reagent Pipettor Z movement (100 cycles). 83.If no errors occur replace the instrument covers. QUALIFICATION OF THE UNIT 84.Perform the usual Qualification Procedure used when a new installation is performed. 85.The UPGRADE is now complete. INOVA Diagnostics, Inc. 9900 Old Grove Road San Diego, CA 92131 United States of America Technical Service (U.S. & Canada Only) : 877-829-4745 Technical Service (Outside the U.S.) : 00+ 1 858-805-7950 E-Mail: [email protected] Page 95 of 95 ECR# 427710