lumox - Sarstedt

lumox
®
Film-based cell culture systems
lumox®
Film-based cell culture system
The lumox® cell culture products stand out for their ultra-thin, gas-permeable film base. As a result of the special film
base design, lumox® products show a very low autofluorescence and a high transparency for exceptionally good
microscopy behaviour.
The range of applications of the lumox® products extends from tissue cultures to automated analysis of fluorescencebased cell assays for active substance testing.
s Low autofluorescence
s High transparency
s Ideal for microscopy
s Gas-permeable film base
s Optimal growth
Optical properties
Low autofluorescence and high light transmission
®
Compared to standard polystyrene bases, the lumox® film
base shows a lower autofluorescence (Fig. 1) and has a
higher light transmission (Fig. 2) than polystyrene or glass
bases.
The low autofluorescence of the lumox film and the
good light transmission enable a consistently high
sensitivity for assays, imaging and reader techniques.
Fig. 2: Light transmission measurement. Detection of low signals,
especially at wavelengths of 200 to 300 nm.
Transmission (%)
relative fluorescence (RFU)
Fig. 1: Fluorescence measurement of lumox® film
and polystyrene bases at 330 nm
lumox® film
Polystyrene base
Glass base
®
lumox film
Polystyrene base
Emission filter (nm)
Wavelength (nm)
Double fluorescence staining of endothelial cells
The superior chemical resistance of the lumox® film enables
a multitude of fixation and staining steps without impairing
its low autofluorescence. In double fluorescence staining
of human endothelial cells, endothelial cell markers,
e.g. Willebrand factor and CD31, are shown clearly and
unaffected by additional signals.
lumox®
Cells simply grow better
The gas-permeable quality of the lumox® film base provides a range of advantages. Cells grow directly at the border
between the gaseous and liquid phase where the culture medium cannot function as a diffusion barrier. While the cells
are directly supplied with oxygen, metabolic waste products like CO2 can simultaneously escape. Exceptionally short
diffusion paths ensure an optimal gas exchange.
Fig. 3: Gas-exchange directly through the lumox® film base
Fig. 4: No gas-exchange in standard tissue culture vessels
through the polystyrene base or glass base
NEW!
lumox® multiwell s The multiwell plate with low autofluorescence
lum
lumox® multiwell plates consists of a black polystyrene frame (standard dimensions)
with a transparent base made from the ultra-thin (50 μm), gas-permeable lumox® film.
Available in 24-well, 96-well and 384-well format.
Order No.
94.6000.014
Description
lumox® multiwell, 24-well
®
Surface
Growth Area per Well
Working volume per Well
Packaging
pcs./case
Standard
190 mm2
500 - 1500 μl
4
2
500 - 1500 μl
20
94.6110.024
lumox multiwell, 24-well
Standard
94.6000.024
lumox® multiwell, 96-well
Standard
34 mm2
25 - 340 μl
4
2
®
190 mm
94.6120.096
lumox multiwell, 96-well
Standard
34 mm
25 - 340 μl
20
94.6000.034
lumox® multiwell, 384-well
Standard
11 mm2
10 - 130 μl
4
94.6130.384
lumox® multiwell, 384-well
Standard
11 mm2
10 - 130 μl
20
lumox® dish s The gas-permeable cell culture dish
lumox® dish is available in a diameter of 50 and 35 mm. With a choice of hydrophilic
or hydrophobic properties, lumox® dish is ideal for cultivation of adherent cells
or suspension cells.
Order No.
Description
Ø/Height in mm
Surface
Working volume
Packaging
Bag/Box
94.6077.305
lumox® dish 50
50/12
Suspension culture
5-10 ml
50
94.6077.410
lumox® dish 50
50/12
Standard
5-10 ml
50
®
94.6077.333
lumox dish 35
35/6
Suspension culture
2.5 ml
50/250
94.6077.331
lumox® dish 35
35/6
Standard
2.5 ml
50/250
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Order Number: 20.215
x-well Cell Culture Chambers
Order Number: 20.417
Order Number: 20.512
!
quadriPERM ®
!
flexiPERM ®
W
NE
Parallel cell culture on up to 4 slides
W
NE
Reusable tissue culture insert
Flexible tissue culture insert
flexiPERM® is a reusable silicone insert to
subdivide slides and cell culture dishes,
e.g. lumox® dish 50 or any other cell
culture dish, into smaller cultivation units.
1
5
The highly adhesive flexiPERM® bottom
adheres to all plain surfaces such as
glass, foil and plastic material.
s Autoclavable tissue culture chamber
from silicone
2
s Highly adhesive to all plain surfaces
(glass, foil, plastic)
4
3
s Cell cultivation, fixation and coloration
s Suitable for DIN slides
s Compatible with flexiPERM® slide and micro12 with
i h or without
i h t slide
lid
Slides made from a variety of materials, PCA, glass and coverglass (170μm), in combination with a polystyrene
frame are shaped as one- or multi-chamber vessels with different growth surfaces.
The product range includes options in a flask or 1, 2, 4, or 8 well format.
s Range of surfaces and formats
s Excludes cell transfer from large cell culture vessels
to slides for microscopical applications
550b-200-0712-0113
Tissue culture dish for parallel analyses
In quadriPERM® cells for cultivation can be easily and
quickly supplied with fresh medium, and the particular
cell growth can be microscopically analysed.
The rectangular, compartmentalized cell culture dish is
designed to accommodate four DIN slides (26 x 76mm).
Applications
quadriPERM® is especially suited for in-situ preparation
of chromosomes by cytogenetic analyses, e.g.
replication studies, immunofluorescence tests (FISH),
and cytoskeleton staining.
The four compartments of identical size enable
parallel cell cultivation under identical conditions.
While suspension cells can be cultivated directly in
the tissue culture dish, adherent cells are cultivated in
combination with coated or non coated slides.
In quadriPERM® the cells can be fixed, stained and
tagged immunohistologically. Accordingly, the cells
can be used for all molecular, genetic and immunological
detection methods.
quadriPERM® in combination
with flexiPERM® slide and
flexiPERM® micro12
s Reduced consumption of media and reagents
Ordering Information
Order No.
Working Volume per Unit
94.6077.307
abt. 10 ml
48
94.6077.308
abt. 10 ml
Packaging
192
Order Number: 20.529
SARSTEDT AG & Co.
P.O. Box 12 20
D-51582 Nümbrecht
Phone +49 2293 3050
Fax
+49 2293 305- 122
[email protected]
www.sarstedt.com
Order Number: 20.536
flexiPERM® slide 1 with eight and flexiPERM® micro12 2 with twelve subdivisions are suited for parallel analyses
of cells on DIN slides. In addition, these two versions can be used in combination with quadriPERM® with or
without a slide.
flexiPERM® conA and flexiPERM® conB
flexiPERM® conA
physiology.
3
and flexiPERM® conB
4
are designed for special cell culture analyses in animal and plant
The cone-shaped form provides numerous applications in micromanipulation/microinjection.
Intra- or intercellular measurements can be performed in parallel microscopic observation.
536c-200-0712-1012
s PCA options with detachable slide leaving no adhesive residues
This document may contain information on products that may not be available in particular countries
Technical modifications reserved
s Excellent microscopic properties
Easy handling
Technical modifications reserved
The x-well cell culture system includes slide-based cell culture vessels for high-resolution microscopy.
This document may contain information on products that may not be available in particular countries
Slide-based cell culture vessels
s Subdivision of slides and Petri dishes etc.
into several growth areas
flexiPERM® slide and flexiPERM® micro12
flexiPERM® disc
The flexiPERM® disc 5 subdivided into four compartments is the ideal insert for the gas-permeable
lumox® dish 50 or any cell culture dish of 50 mm diameter.
flexiPERM® disc can be used for co-cultivation of different cell lines in one vessel.
* flexiPERM® can be autoclaved at 121°C for 20 min. without loss of its mechanical properties.
Users must determine if the intended application will have any adverse effect on other product properties.
Ordering Information
Order No.
Description
Fig.
Cultivation Units
Cultivation Area per Unit
Packaging
94.6011.436
94.6032.039
94.6034.067
94.6077.434
94.6077.435
flexiPERM® micro12
flexiPERM® slide
flexiPERM® disc
flexiPERM® conA
flexiPERM® conB
2
4
12
8
4
1
1
0.3 cm2
0.4 cm2
1.8 cm2
1.1 cm2
3.1 cm2
5
5
5
5
5
1
5
3
SARSTEDT AG & Co.
P.O. Box 12 20
D-51582 Nümbrecht
Phone +49 2293 305-0
Fax
+49 2293 305-122
[email protected]
www.sarstedt.com
Order Number: 20.536