Issue - Southeastern Microscopy Society

V OLUME 22, I SSUE 2
W INTER 2007 V 1.0
SEMS 2008—A PRIL 16-18—P ENSACOLA B EACH , FL
M
ark your calendars for the next annual meeting of the Southeastern
Microscopy Society! This year we will be
meeting in beautiful Pensacola Beach at the
Hilton Pensacola Beach Gulf Front Hotel.
We expect a fabulous list of speakers (as
always) and hope to have all our corporate
members and sponsors present with lots of
nifty toys to ogle (if you’re into that sort of
thing). Please make sure you make your
reservation requests by March 18, 2008 to
ensure that you are given the special discounted rate. With the SEMS discount the
following rates should apply:
Soundside Standard:
Gulf Front Standard:
Tower 1BR Suite:
$139
$169
$199
It was mentioned that if your institution is a
tax free organization you won’t have to pay
the hotel room tax (around 14%) so be sure
and let them know!
Click HERE to go directly to the SEMS
2008 meeting page with all the information.
SEMS 2008 Program Chairs:
Dr. John Shields
University of Georgia
Dr. Charles Humphrey
Centers for Disease Control
Local Arrangements Committee:
Mr. Bill Monroe
Mississippi State University
Ms. Cynthia Goldsmith
Centers for Disease Control
I NSIDE
Ms. Amanda Lawrence
Mississippi State University
L ETTER F ROM
THE P RESIDENT
Ruska Chair
Ms. Jeannette Taylor
Emory University
THIS ISSUE :
A B RIEF H ISTORY 6
OF S EMS
2007 R USKA
7
A WARD W INNERS
Events of Interest
S
3
11
W ISH L IST 2007
ince it’s that time of year when most of
us think hard about what we’d like to
find under the Christmas tree (if you’re into
that), I thought I’d make a list of some of the
nifty gadgets or technologies that I would
love to have readily available at home or at
work.
Mini EM
Who wouldn’t want there very own miniature SEM or TEM? I mean, come on! You
could put one these in the trunk of your
pimped out Ford Focus, park at Hardees's
and you would soon be the envy of all the
locals. Hitachi and FEI both make tabletop
SEMs (the TM-1000 and Phenom, respectively) and Delong Instruments manufacturers the LVEM5– a tabletop SEM, TEM and
STEM. Of course there are drawbacks to
these instruments such as fixed accelerating
voltage, small sample size and backscatter
detection only; but it can fit in your passenger seat! (continued on page 4)...
C ALL
FOR
P APERS 5
C ALL
FOR
P APERS 5
R USKA A WARD
I NFORMATION
8
T RAVEL S UPPORT
A PPLICATION
12
I NSIDE S TORY
6
1
Executive Council
President:
Robert Simmons
Department of Biology
Ga State University
Atlanta, GA 30303
404.651.3138
[email protected]
President-Elect:
Giselle Thibaudeau
Dept. Biological Sciences
Mississippi State University
Mississippi State, MS 39762
662.325.7572
[email protected]
Past President:
Johnny Carson
Dept. Pediatrics
Univ. North Carolina
Chapel Hill, NC 27599
919.966.0762
[email protected]
Secretary:
Cynthia Goldsmith
1600 Clifton Rd.
CDC MS G30
Atlanta, GA 30333
404.639.3306
[email protected]
Treasurer:
Karen Kelly
University of Florida
PO Box 110700
Gainesville, FL 32611
352.392.1184
[email protected]
Corporate Member-at-Large:
William A. Monroe
PO Box 2190
Mississippi State Univ.
Mississippi State, MS 39762
662.325.3019
[email protected]
Member-at-Large:
Member-at-Large:
Jeanette Taylor
803 Inland Way
Lilburn, GA30047
770.717.8456
[email protected]
Amanda Lawrence
Mississippi State University
Mississippi State, MS 29762
662.325.3019
[email protected]
Appointed Officers
Historian:
Gray Jerome III
Dept. Pathology
Vanderbilt Univ. Med. Center
Nashville, TN 37232-2561
615.343.7023
[email protected]
Photographer:
Dayton Cash
Electron Microscopy Facility
Clemson University
Anderson, SC 29625
Webmaster:
Patrick Brown
Dept. of Biology
King College
Bristol, TN 37620
423.652.4805
[email protected]
Newsletter/Proceedings Editor:
David Burk
Pennington Biomedical Res. Center
6400 Perkins Road
Baton Rouge, LA 70808
225.763.2779
[email protected]
Investments:
Charles Humphrey
1600 Clifton Road
CDC DVRD MS G32
Atlanta, GA 30333
404.639.3306
[email protected]
864.656.2465
[email protected]
Newsletter of the
Southeastern Microscopy Society
A local affiliate of:
The Microscopy Society of
America, The Microbeam Analysis
Society and the Association of
Southeastern
Biologists.
The Beam is published approximately
three times a year for members and
friends of the Southeastern Microscopy
Society.
Copyright 2007 Southeastern Microscopy
Society
www.southeasternmicroscopy.org
2
L ETTER FROM THE P RESIDENT
December 20, 2007
Dear SEMS Members,
Welcome to the maiden voyage of the new electronic publication of The Beam. Council brought a proposal to the membership that The Beam join the electronic age and move to an Internet-based format for
publication. Our 2007 annual meeting in Decatur, GA, was a success with the largest turnout that SEMS
has seen for quite a while. Look for more changes and new services on our web site in the coming
weeks.
In the last year I have had to opportunity to attend meetings with several of our sister societies in such far
-flung places as New Mexico, California, Oklahoma, Ohio and North Carolina (some maybe not so farflung). Many professional societies have struggled with membership issues over the last few years, but I
am happy to report that things are looking good across the country. I also learned that SEMS is not alone
in recognizing student excellence. The Oklahoma Microscopy Society sponsors the Timpano student
competition in the same spirit as our own Ruska as well as the annual Ugly Bug Contest in support of
secondary science education. I enjoyed some very good student papers and saw some great micrographs
during my visit to their Fall meeting in Tulsa.
I want to remind everyone that the Microscopy and Microanalysis meeting will be held in Albuquerque,
NM, in August of 2008. Albuquerque is a great city and we can expect to have a very good time there,
even is it is high summer. They tell me that it’s dry heat and isn’t so bad. The Program Committee has
put together a program which should have many things of interest to microscopists from all of the disciplines. I hope to see a strong SEMS contingent there this summer. Also remember to mark your calendars for the SEMS meeting in sunny Pensacola Beach in April. Bill Monroe and Cynthia Goldsmith
have worked hard to get us accommodations on the beach again at the Hilton Garden Inn where we met a
few years ago. John Shields and Charles Humphrey are organizing a program that we’re all sure to enjoy.
I have also been reminded this year of just how quickly time slips away and it is again the time when we
reflect upon the past year. Remember the good times and appreciate the colleagues, friends and families
that we all hold dear. I wish you all the happiest of holidays and a prosperous New Year.
See you at the beach in April!
Robert Simmons
SEMS President 2007
3
WishList 2007
(continued from page 1)
Prices seem to range from ~$60K up to $150K for the Delong
depending on options.
From left to right: Hitachi TM-1000, FEI Phenom, Delong LVEM5.
Serial Block-Face Scanning
CAKE
EAT
Location of NTEGRA
Tomo and Gatan 3View
in a classic Venn diagram.
By merging an ultramicrotome with an imaging modality you
get either the Gatan 3View or the NTEGRA Tomo. With the
3View you get a device that serial sections inside a variable
pressure field emission SEM allowing for precise registration
between sections for 3D rendering. The Tomo is essentially a
Leica EM UC6 ultramicrotome coupled with an atomic force
microscope—as you section your block the face is scanned via
AFM and you can reconstruct the specimen in 3D.
Spectral Imaging
This technology is quite old and pretty much any confocal you
buy now and days comes equipped with the ability to do spectral scans of your sample (Zeiss 510 META, Leica TCS SP5,
Nikon Eclipse C1, Olympus FluoView) and you can even convert your standard widefield setup to do spectral imaging too
(CRi’s Nuance, Mag Biosystems SpecEM). So far I’ve been
unable to dig up someone doing excitation scanning in conjunction with a spectral emission scan. Maybe because it’d
take to long? Regardless, spectral scanning can come in handy
when you’re trying to remove autofluorescence signal from
your sample or if you need to separate FITC from GFP. It can
even be used on your brightfield images! I include this in my
list as I think the benefits of this technology warrants it’s availability in a microscopy core.
Right: Zeiss’ Colbri
LED system and
COOLLED’s precisExcite.
LEDs in conjunction
with the increase in
luminous output there are now several companies that make
fluorescence modules that can replace your typical arc-lamp.
Zeiss sells the Colibri which can hold 4 different LEDs (there
are now 10 different colors available from Zeiss) and
COOLLED sells the precisExcite - a fluorescence module that
looks a lot like a standard metal halide burner but holds 3 LED
modules of different excitation wavelength. Since LEDs
power on and off so rapidly this technology is great for live cell
imaging. LEDs also have a fantastically long life time (the precisExcite modules have 3-year warranties) which makes up for
the sticker shock of the individual light modules.
Helium Ion Microscope
Let’s see….better resolution,
don’t need to coat your sample,
little if any specimen damage,
extremely low imaging currents—
check. The next generation scanning EM is here thanks to technology capable of generating a gas
field ion source. Since helium ions have a much larger mass
than electrons used in current EM scopes (and hence, a much
smaller wavelength) these instruments should be capable of a
dramatic increase in overall resolution when compared to their
standard FEG counterparts—maybe much greater than 10
times better. Currently it appears that the only consumer
product available is the ORION from Carl Zeiss but that
should change as time progresses.
CyScope
I got to see this while at the 3D Microscopy course in Vancouver. Iain Johnson from Invitrogen had one set up
and it was a definite crowd-pleaser. A perfect stocking-stuffer—this scope is set up for either UV or
blue fluorescence AND transmitted light (via LED)
and has an optional USB camera you can pop on.
It’s made primarily for in-the-field TB and malaria
testing but I think it looks awesome anywhere—
from the dinner table to the tailgate party. It weighs
just a tad over 2kg and is a brilliant emerald green to
LED Illumination
boot! You can find them at Partec.com for around a thousand
Now that LEDs come in 5 watt (and even 10W) versions with bucks. My address is located elsewhere in this publication for
>120 lumen output it was only a matter of time before they
those who want to send me one.
were integrated as light sources into microscopes. Since there
DHB
has been a steady increase in the number of available colors of
4
Sems micrograph competition
Sponsored by The Beam
$50 Prize for the best LIGHT MICROGRAPH
$50 Prize for the best ELECTRON MICROGRAPH
RULES FOR ENTRY
1.You must be registered for the entire SEMS meeting.
2. Bring your micrographs with you to the meeting. You may submit a maximum of two entries (no
individual may win more than one prize).
3. Your entries must be posted by 10 am, April 16.
4. Overall dimension is limited to 11”x14” (less is better).
5. A separate sheet of text should include the title and a brief description of your micrograph(s).
6. Please print your name and contact information on the back of each micrograph.
Entries must be scientifically significant and will be judged on the basis of aesthetics
and technical skill.
WINNERS WILL BE ANNOUNCED AT THE BANQUET.
Winning micrographs will be published in The Beam.
If you need more information please contact
Beth Richardson
706.542.1790 or [email protected]
SEMS 2008 Micrograph Competition Winning Entries:
David H. Burk—Autofluorescence and GFP-tubulin fluorescence in Arabidopsis seedling taken with a Zeiss Lumar stereomicroscope (left). Bill Monroe—This
Scanning Electron Micrograph is an image of the surface pattern of a sex scale, which releases pheromones on the hind leg of a male tortricid moth to attract
mates. The genus of the moth is Thaumatotibia and was obtained in Thailand. The micrograph was imaged on a JEOL JSM 6500F Field Emission Scanning
Electron Microscope (right).
5
Historical Perspective
Edited by Jay Jerome
This is another in a recurring feature of the Beam dealing with our history, membership, and the Socieity’s contributions to
science. I hope every SEMS member (young and old) will consider contributing to this feature. I see this as a space to share our
history, get to know each other better, and record the personal side of science. The submission does not have to be long, just
interesting. Send submissions to me at [email protected].
A Brief History of SEMS 2005
by
Bill Monroe
I certainly wish I could start this story…Once upon a time…. but unfortunately the story I will relate now of 2004 and 2005
was very real indeed.
To begin the story leading up to the 2005 SEMS Meeting, which was finally located in Pensacola Beach Florida, let me begin
with a brief history leading up to that year’s meeting.
First, let’s go back in time to a simpler era, an era almost 15+ years ago, when a fellow staff member here at MSU volunteered
to help with Local Arrangements for an Alabama EM Society (AEMS) meeting. Most of my memory of her duties as LAC member involved the purchase of large bottles of liquor and set-ups for the receptions. Meetings back then for AEMS were small,
simple affairs as compared to the larger SEMS and Texas Society for EM meetings I have attended.
So in 2004, when Charles Sheetz suggested a meeting in Gulf Shores, Alabama for 2005, I quickly volunteered to be Local Arrangements Chairperson if the meeting were to be held there (my family and I had vacationed there for 16 years in a row, so we
knew the area well).
Being naïve about volunteering to be the LAC, the memory of just buying a few large bottles of booze and set-ups quickly faded
as the secretary gave me the SEMS operation manual, which in precise terms described the LAC Duties in exquisite detail.
I quickly became familiar with exciting new terms like; group contract, guest room block, room rates, room taxes, room
comps, meeting room set-up cost, exhibits, morning and afternoon breaks, corporate sponsored reception, signage, banquet
reception, banquet food choices, business buffet breakfast, etc etc.
So like any good SEMS member, I quickly threw myself into the task set before me.
My wife and were excited that we could combine business travel and pleasure while acquiring the contract on the coast. That
excitement quickly faded in the next few months as the following time line indicates.
•
First Contract:
Quality Inn, Gulf Shores, Alabama Spring 2004.
•
Contract Cancelled:
Summer 2004 (Hotel purchased and slated for destruction for condo development).
•
Second Contract:
Hampton Inn, Pensacola Beach, Summer, 2004.
•
Hurricane Ivan:
Landfall, September 16, 2004.
Results: 650 Homes in area destroyed, 2 Hotels left functioning on Pensacola
Beach.
•
2nd Contract Cancelled:
January, 2005
•
3rd Contract:
Hilton Garden Inn, Pensacola Beach, January 2005
•
SEMS 2005 Meeting:
May 18-20, 2005
In total, as LAC for 2005, I made 4 trips to the Gulf Coast and visited 7 Hotels, of which I stayed in 4 while checking out the
facilities. 3 Hotel Contracts were acquired, 2 of which were cancelled, for reasons noted above.
6
Summary:
As I think back to all the travel,
contracts, cancelled contracts,
and especially Hurricane Ivan of
2004, there are 2 memories’
that still haunt me.
1. The employee at the Hampton who sat in an empty hotel,
that had been ravaged by a hurricane, telling me over the
phone that the hotel would not
re-open, our contract was void
and she herself was waiting for
her job to be terminated.
2. The staff of the Hilton, who
sat across from me during contract negotiations with no smiles
as they continued to do their
Color-enhanced composite satellite image courtesy NASA-GSFC, data from NOAA GOES
jobs while their homes were
either destroyed or in repair.
In the end, the memories of 2004 and 2005 are forever etched in my memory.
Ruska Award Winners
R
uska Award winners at the SEMS 2007 meeting in Decatur, GA were Jennifer Seltzer (left) from the Department of Sociology, Anthropology and Social Work at Mississippi State University and Tao Wu from the Woodruff School of Mechanical Engineering, Georgia Tech in Atlanta, GA (right). Jennifer won the prize in the field of biological sciences with her presentation entitled Answering the Osage Problem: Using Scanning Electron Microscopy to Identify Archaeological Charcoal and Tao won in the
field of materials science with his entitled Modifying Bond Kinetics of VWF Adhesion by Eliminating Catch Bonds. Congratulations to
them both on their excellent work!
7
2007 Ruska Award Winner in Materials Science
Tao Wu
Modifying Bond Kinetics of VWF Adhesion by Eliminating Catch Bonds
Tao Wu,1* Jun Yang,1*† José A. López,2 Miguel A. Cruz,3 and Cheng Zhu1,4,5
1
Woodruff School of Mechanical Engineering, 4Coulter Department of Biomedical Engineering, 5Parker H. Petit Institute for
Bioengineering and Bioscience, Georgia Institute of Technology, Atlanta, GA, USA, 2Puget Sound Blood Center, Seattle,
WA, USA, 3Baylor College of Medicine, Houston, TX, USA , †Present Address: University of Western Ontario, London, ON,
Canada
*
These authors contributed equally to this work
Introduction
The binding of platelet glycoprotein (GP) Ib-IX-V to its ligand immobilized on the exposed subendothelium surface, von Willebrand factor (VWF), initiates the first step of hemostasis to recruit patrolling
platelets in the blood to damaged vascular surface and also thrombi formation underlying cardiovascular
disease and stroke [1]. There are three types of VWD resulting from qualitative or quantitative deficiency
in VWF. Type 2B VWD has been characterized related to a gain-of-function phenotype with increased
affinity with GP Ibα and removal of ULVWF from the circulation [2].
Catch bond has been revealed to govern the flow enhancement of L-selectin mediated leukocytes adhesion [3]. Similar to leukocytes, platelets also require a critical level of hydrodynamic flow to initiate
adhesion to VWF-A1 but this requirement was abolished for type 2B mutations [4, 5]. In this study, we
observed for the first time that GP Ibα-A1 interaction exhibits catch bond under force using Atomic
Force Microscope (AFM). However, two type 2B VWD mutants A1 R543Q and R687E totally eliminated the catch bond giving rise to slip bond behavior only, suggesting that catch bonds between A1 and
GP Ibα may prevent adhesion of circulating platelets to surface immobilized VWF-A1, while type 2B
mutant facilitated the platelets aggregation. From the single molecular kinetics measurement, our findings provide a mechanism explanation for the flow enhancement of platelets adhesion and type 2B
VWD.
Materials and methods
Soluble GP Ibα (glycocalicin) was purified from human platelets. WT and mutant A1 (R543Q and
R687E) proteins were produced in Escherichia coli and purified. A custom AFM was used to measure
lifetimes of GP Ibα-A1 bonds. A1 was physically adsorbed on the Petri dish and GP Ibα adsorbed on the
cantilever tip. The cantilever tip was allowed to contact the petri dish for 0.02 s, then retracted ~4 nm
above the surface and held for 1 s to allow bond formation. The cantilever was then further retracted for
rupture event and lifetime measurements.
Results
The dependence of binding frequencies on the distances from the Petri dish surface has been characterized for both non-specific and specific binding, which is BSA and GP Ibα interacting with WT/mutant
A1 (A1-R543Q data was shown in Fig. 1A. Non-specific binding frequencies decreased rapidly below
5% when the distance between cantilever tip and petri dish surface approached 4 nm. However, the specific binding was relatively stable and could still maintain around 15% when the distance was larger than
4nm. Binding specificity of WT/mutant A1 with GP Ibα has also been verified through A1 antibody 5D2
blocking experiments (Fig. 1B).
For each of three (WT, R687E and R543Q) A1 interacting with GP Ibα, >500 lifetimes were collected
and sorted by different force bins. Average lifetime and force value were taken as representative lifetime
8
under that force in each force bin. WT VWF-A1-GP Ibα bond showed a biphasic catch-slip transition at
~22 pN, which gave a transitional longest lifetime ~0.3 second (Fig. 2). Remarkably, the naturally occurring type 2B VWD mutant R543Q differed dramatically from those for WT A1, which totally eliminated
the catch bond and only showed slip bond behavior. At very low forces, lifetimes were prolonged and
monotonically decreased with increasing force. The mean bond lifetime vs. force curve for R543Q mutant collapsed with that for WT VWF-A1 as force increased above 40pN, where WT A1 catch bonds has
already transited to slip bonds. Another single residue gain-of-function mutant R687E, constructed mimicking type 2B VWD phenotype, also showed total elimination of catch bond at forces below optimal
force of WT A1 (~22 pN). The R687E slip bond curve also collapsed with WT and R543Q’s curves at
forces around 40pN. The prolongation of lifetime for R687E mutant under nearly zero forces was more
evident than the R543Q mutant, which gave 1.5 seconds and 0.3 second under ~5pN for R687E and
R543Q, respectively.
Discussion
Since being theoretically proposed about two decades ago [6], the counterintuitive catch bond hasn’t
been observed experimentally until recently [3, 7, 8]. Selectin-ligand catch bond has been demonstrated
to govern the L-selectin mediated adhesion and rolling of leukocytes under threshold shear [3]. Flow enhanced adhesion has been observed not only in selectin mediated cell adhesion, but also in VWF-A1-GP
Ibα mediated platelets tethering and rolling adhesion on injured endothelial surface. Previous kinetics
studies demonstrated the VWF-A1-GP Ibα tether bond possess all the biomechanical properties
associated with the selectin family, including flow dependent adhesion and rapid and force-dependent
kinetics [4]. Thus, it is most likely for one to speculate that VWF-A1-GP Ibα bond also shows catch
bond. This hypothesis has been proven in this study. The observation of catch bond in GP Ibα-A1 system
is not unusual if considering the similarities with selectin-ligand system. Point mutations on GP Ibα or
A1 have been identified and found related to many bleeding disorder, like platelet-type and type-1, type 2
(A, B, M) VWD. Our results provided kinetic insights in understanding how those structural changes
could lead to bond behavior change and thus the clinical consequences.
We thank Dr Robert K. Andrews (Monash University, Clayton, Australia) for the 5D2 antibody. We thank Mr Fang Kong for
help with the AFM force-clamp programming.
Reference:
1.
2.
3.
4.
5.
6.
7.
8.
9.
Savage, B., E. Saldivar, Z. M. Ruggeri, Initiation of platelet adhesion by arrest onto fibrinogen or translocation on
von Villebrand factor. Cell, 1996. 84: p. 289-297.
Ruggeri, Z.M., R. Lombardi, L. Gatti, R. Bader, C. Valsecchi, T. S. Zimmerman, Type IIB von Willebrand's disease:
Differential clearance of endogenous versus transfused large multimer von Willebrand Factor. Blood, 1982. 60: p.
1453-1456.
Yago, T., J. H. Wu, C. D. Wey, A. G. Klopocki, C. Zhu and R. P. McEver, Catch bonds govern adhesion through Lselectin at threshold shear. J Cell Biol, 2004. 166: p. 913-923.
Doggett, T.A., G. Giridhar, A. Lawshe, D. W. Schmidtke, I. J. Laurenzi, S. L. Diamond, T. G. Diacovo, Selectin-like
kinetics and biomechanics promote rapid platelet adhesion in flow: the GPIbα-vWF tether bond. Biophys. J., 2002.
83: p. 194-205.
Doggett, T.A., G. Giridhar, A. Lawshe, J. L. Miller, I. J. Laurenzi, S. L. Diamond, T. G. Diacovo, Alterations in the
intrinsic properties of the GPIbα-vWF tether bond define the kinetics of the platelet-type von Willebrand disease mutation. Blood, 2003. 102: p. 152-160.
Dembo, M., D.C. Tourney, K. Saxman and D. Hammer, The reaction-limited kinetics of membrane-to-surface adhesion and detachment. Proc. Royal Soc. London, 1988. 234: p. 55-83.
Marshall, B.T., M. Long, J.W. Piper, T. Yago, R.P. McEver, and C. Zhu, Direct observation of catch bonds involving
cell-adhesion molecules. Nature, 2003. 423: p. 190-193.
Sarangapani, K.K., T. Yago, A. G. Klopocki, M. B. Lawrence, C. B. Fieger, S. D. Rosen, R. P. McEver, and C. Zhu,
Low force decelerates L-selectin dissociation from P-selectin glycoprotein ligand-1 and endoglycan. J Biol Chem,
2004. 279: p. 2291-2298.
Kumar, R.A., J. F. Dong, J. A. Thaggard, M. A. Cruz, J. A. Lopez, L. V. McIntire, Kinetcs of GPIbα-vWF-A1 tether
bond under flow: effect of GPIbα mutations on the association and dissociation rates. Biophys. J., 2003. 85: p. 40994109.
9
-4
0.4
GPIb
BSA
0.3
0.2
0.1
0
-2
0
2
4
6
8
10
Binding Frequency(%)
B
Binding Frequency(%)
A
BSA
GPIb
GPIb+5D2
0.30
0.25
0.20
0.15
0.10
0.05
0.00
WT
R687E
R543Q
Distance(nm)
Figure 1 Binding specificity control through distance and mAb blocking effect.
Figure 2 Mean bond lifetime vs. force for WT and mutant
A1 vs. GP Ibα measured by AFM.
10
Events of Interest
March 1-7, 2008
PITTCON 2008
New Orleans, LA
www.pittcon.org
June 2-6, 2008
Ph.D. Course: Confocal Light Microscopy
Center for Advanced Microscopy
University of Amsterdam, The Netherlands
March 4-8, 2008
7th Annual FRET Microscopy Workshop
W.M. Keck Center for Cellular Imaging
University of Virginia
June 5-14, 2008
UC Berkeley Cryo EM Course
UC Berkley, CA
April 13-16, 2008
Focus on Microscopy
Osaka-Awaji, Japan
http://www.focusonmicroscopy.org
April 14-17, 2008
SCANNING 2008
FAMS, Inc.
Bethesda, MD
www.fams.org
April 16-18, 2008
SEMS 2008
Hilton Pensacola Beach Gulf Front Hotel
Pensacola Beach, FL
www.southeasternmicroscopy.org
May 7-16, 2008
Analytical & Quantitative Light Microscopy
Marine Biological Laboratory
Woods Hole, MA
May 31– June 6, 2008
Quantitative Fluorescence Microscopy
Mount Desert Island Biological Laboratory
Salisbury Cove, ME
June 14-26, 2008
3D Microscopy of Living Cells
University of British Columbia
Vancouver, BC, Canada
August 3-7, 2008
Microscopy and Microanalysis 2008
Microscopy Society of America
Albuquerque, NM
September 17-19, 2008
14th Annual Workshop on Single Molecule Spectroscopy and
Ultra Sensitive Analysis in the Life Sciences
PicoQuant GmbH
Berlin, Germany
October 7-16, 2008
Optical Microscopy & Imaging in the Biomedical Sciences
Marine Biological Laboratory
Woods Hole, MA
December 13-17, 2008
The American Society for Cell Biology
San Francisco, CA
www.ascb.org
June 2-6, 2008
Scanning Electron Microscopy and X-ray Microanalysis
Lehigh University
Bethlehem, PA
HAPPY NEW YEAR!
11
SEMS Business Meeting
Friday, April 13, 2007
Holiday Inn Hotel, Decatur, GA
SEMS President Johnny Carson opened the meeting at 7:38 AM.
Members present: Shampa Aich, Mary Ard, Rich Brown, David Burk, John Caola, Johnny Carson, Glenn Cohen, Cynthia
Goldsmith, Raymond K. Hart, Charles Humphrey, Jay Jerome, Karen Kelley, Sara Miller, Vera B. Larke, Amanda M. Lawrence, Charles W. Mims, Alan Nicholls, Beth Richardson, Elaine Schumacher, Jennifer Seltzer, John Shields, Robert Simmons,
Jeannette Taylor, Giselle Thibaudeau, Tao Wu.
SEMS Archives
- Jay noted that the Society has a dearth of pictures, so please let him know if you have pictures from past meetings (or perhaps
embarrassing pictures of SEMS members). These help to put a human face on the Society.
A call was put out whether anyone knows the whereabouts of Adele Mills, who has many of the old pictures.
- Jay called attention to the series of remembrances in the BEAM, and encouraged members to send in their own reflections.
- Lastly, putting on his hat as MSA Past-President, Jay urged members to encourage students to attend Microscopy & Microanalysis 2007, and noted that bursaries are available.
Treasurer’s Report, Karen Kelley
- This year’s books are looking much better, and the treasury is on the road to recovery.
- The list of members has been cleaned up, excluding the members in arrears, so that the membership’s numbers better reflect the true numbers.
- The Corporate membership is strong.
- Karen thanked the Local Arrangements Committee and Bill Monroe for the strong Corporate turnout at the 2007 meeting.
- The Treasurers Report was accepted as written.
- Johnny thanked Karen for her great stewardship of this position.
Secretary’s Report, Cynthia Goldsmith
- The minutes from the previous General Business meeting were accepted as written.
Endowments Report, Charles Humphrey
- Current balance is $37,790.06, which includes a gain of $1911.03 in 2006.
- Johnny thanked Charles for his stewardship of the SEMS Endowment for so many years.
Election Results, Robert Simmons
- Amanda Lawrence has been elected as Member-At-Large, who is replacing Rich Brown as he rotates off the SEMS Executive Council.
- Cynthia Goldsmith has been re-elected as Secretary.
- Giselle Thibaudeau has been elected as the new President-Elect.
Upcoming Meetings
- Bill Monroe announced that the SEMS 2008 meeting will be held in Pensacola, Florida, on April 14-18.
BEAM Newsletter / SEMS Website
- Johnny noted that David Burk has carried the flag for the Newsletter and Proceedings.
- Johnny informed the membership that the Executive Council has wrestled with the issue of whether to continue publishing a
hard copy of the BEAM. It was brought before the Membership to recommend that the hard copy be disbanded and go entirely digital.
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- Additional Points:
There will still be a strong need for a BEAM Editor and a Webmaster.
The BEAM would be sent as a pdf-file.
The Society would still have income from the corporate members for advertisements, and that the ads could contain weblinks to their company websites.
- A motion was made that the hard copy of the BEAM be disbanded.
- Discussion:
Giselle inquired if the corporate members have been asked their opinions.
-Johnny noted that the hard copy of the ads have always been a headache, and this would make things easier.
Amanda wondered about the savings from not having the expenses for printing and bulk mailing.
Karen thought that at least 25-50 copies should be printed out, for the archives, new members, and to display at meetings.
-John S. observed that it can cost as much to print 25-50 copies as to print a much higher number.
Rich B. thought that the Corporate members would be thrilled at the prospect of not having to print off the insets.
It was pointed out that the Webmaster would need to ensure that all links are up-to-date.
Sara pointed out that some people could print out the BEAM on their own computers.
Sara also initiated a discussion about the fact that at this time, Google is not bringing up the current SEMS website. We
need the Website to be easy to find, to help bring in more members.
-John S noted that there are still 3 different domains for the SEMS website.
-Rich pointed out that there are some things that Patrick can do to improve the situation with the search engines.
Charles M. wondered if the Proceedings should also be online.
-It was pointed out that there is an ISBN number for the Proceedings, and how this might be affected.
-Putting the Proceedings online was tabled, for lack of current knowledge.
- The motion to disband the hard copy of the BEAM in favor of going digital was passed unanimously.
Interaction with MSA and Other Societies
- It was reaffirmed that we value our relationship with MSA. Any members who wish to volunteer for MSA would be put
right to work.
- Another local society in close proximity for many SEMS members is AReMS (Appalachian Regional Microscopy Society).
Their annual meeting is usually in the fall, and members were encouraged to interact with colleagues in this Society.
Closing Remarks, Johnny Carson
- A key to success is a good Local Arrangements Committee, and Johnny thanked Robert, Charles H., and Cynthia for bearing the weight this year.
- Johnny sent out his acknowledgements and thanks to:
Ann Ellis and David for the Program and Proceedings
The students, and the ability of the Society to nurture and foster research.
Rich and Jeanette for heading up the Ruska Award Committee.
Our invited speakers, Jay Jerome, Elaine Schumacher, Alan Nicholls, and Rich Brown.
Bill Monroe as Corporate Liaison
Our Corporate Members, and especially Doug Griffith who was recognized this year.
- Lastly, Johnny conveyed his thanks for being elected President. He has many friends in SEMS, and he noted that we are still
a vital organization.
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Remarks by Incoming President, Robert Simmons
- Gave his thanks to the other members of the LAC, and to Karen and Amanda for their time on the Front Desk.
- Noted that the vendor area is still open.
He hopes to add in more time to visit the vendors in the 2008 Program.
- Closed with a “Thank you” to Johnny, for keeping us on track this past year.
Meeting adjourned at 8:15 AM.
HISTORY QUIZ #1
The image above has several photographs taken during the 1986 SEMS meeting in Savannah Georgia (Photos courtesy of
Charles Mimms). Your task, should you choose to accept it, is to correctly identify the 10 individuals indicated by numbers.
You can email your answers (guesses) to me at [email protected]. Please use History Quiz #1 as the subject line and
match names to corresponding number (i.e. don’t just send me a list of random names). There will be a prize to the winner.
Don’t ask what it is, it is a secret. To be eligible, your answer must be received within two weeks of the BEAM mailing date.
You must be a SEMS member in good standing (or at least sitting down) to enter. Only one entry per person allowed. In the
unlikely event that more than one person gets all of the individuals correct, the winner will be determined by an unbiased and
random selection of a winner from among those getting the correct answers. In the event that no one gets all the names correct, the person with the most correct answers will be declared the winner. The winner will be announced (exposed) in the
next issue of the BEAM. I’ll begin by giving you a hint. Person number 2 is not Sly Stone!
Good luckJay Jerome
SEMS Historian
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Astigmatisms
(and Contests!)
The following was submitted by John Shields:
Some of us have, at one time or another, been unfortunate enough to look for employment. And most of us had a wall wallpapered with rejection letters. The following adapted letter was noted from Steve Chilton’s site at the Univ. of Minnesota Duluth and should be of help the next time you discover that special place to be. Be sure to make the necessary editing before
sending.
March 14, 2008
Professor Tutwhittle
Chair - Search Committee
Department of Biology
University of Georgia
Athens, GA
Dear Professor Tutwhittle,
Thank you for your letter of March 6. After careful consideration, I regret to inform you that I am unable to accept
your refusal to offer me an assistant professor position in your department.
This year I have been particularly fortunate in receiving an unusually large number of rejection letters. With such a
varied and promising field of candidates it is impossible for me to accept all refusals. Despite the University of
Georgia's outstanding qualifications and previous experience in rejecting applicants, I find that your rejection does
not meet my needs at this time. Therefore, I will assume the position of assistant professor in your department this
May. I look forward to seeing you then.
Best of luck in rejecting future applicants.
Sincerely,
Evan I. Werk
Two Contests!
John Shields provided the image at the bottom left—correctly identify it and you win a SEMS USB flash drive!
The lower right image was sent in by Bill Monroe—identify the structure in the center of the photo tentatively titled “make a wish” and you win a PINIA COLADA!!!
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Left: Robert Simmons shows off
the IPOD nano he won in the
Hitachi TM-1000 contest.
Right: Doug Griffith being
honored as a Distinguished Corporate Member through his association with SEMS by representing Hitachi America.
Above left: Glenn Cohen and John Shields enjoy a coffee before the business breakfast meeting gets started. Above right: Many
Ruska candidates, Jay Jerome, Dayton Cash, Doug Griffith and Bill Monroe rally under their particular brand of flag.
Below left: Cynthia Goldsmith, Charles Humphrey, Johnny Carson and Robert Simmons at the Decatur courthouse before the delicious banquet. Below right: Jay Jerome is either giving a history lesson on how microscopists used to travel to conferences “back in
the day” or is trying to tally the number of beers Bill has so far consumed.
Thanks to Dayton Cash for providing the photos from SEMS 2007 as well as caption information!
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SEMS 2008
CALL FOR PAPERS
The 2008 Annual Meeting of the Southeastern Microscopy Society to be held at The Hilton Pensacola Beach
Gulf Front Hotel in Pensacola Beach, FL. April 16 –18, 2008.
Deadline for receipt of abstracts (Including Ruska Award Participants) has yet to be determined
Questions or problems with preparation of abstracts - please contact the Proceedings Editor (David Burk,
phone: 225.763.2779 or e-mail: [email protected]).
Preferred methods of submission:
A. Disk with submission
B. E-mail /attachment: Formats accepted - MSWord, RTF, TXT. Please avoid PDF files.
Complete the attached form indicating the type of presentation (Platform or Poster) and projection requirements (35 mm projector, overheads,or computer with Powerpoint -Windows platform only).
Posters must fit on 4’ tall x 8’wide poster space.
Abstract Submissions to (e-mail preferred):
Dr. David Burk
Cell Biology and Bioimaging Core
Pennington Biomedical Research
Center
6400 Perkins Road
Baton Rouge, LA 70808
Phone: 225.763.2779
e-mail: [email protected]
Dr. John Shields
Center for Ultrastructural Research
151 Barrow Hall
University of Georgia
Athens, GA 30602
Phone: 706.542.4080
e-mail: [email protected]
Dr. Charles Humphrey
Centers for Disease Control
1600 Clifton Road
CDC DVRD MS G32
Atlanta, GA 30333
Phone: 404.639.3306
e-mail: [email protected]
INSTRUCTIONS for Preparation of Abstracts:
1. Draw a box (for formatting purposes only) (4 5/8 ” x 7 1/8 “) as illustrated for the attached sample abstract on the following page.
Do not include the lines of the box in your final submitted
copy!
2. Single space for all text. 12 point Times Roman or Times New Roman font only. This will insure uniformity of space and format for the
Proceedings.
3. Indent the title of the abstract, authors and their affiliations 0.4 inches
from the left margin. Type as far as possible up to, but not crossing, the
right margin. BOLD all letters in the title. Italicize or underline scientific
names. Please do not list degrees of the authors.
4. Start the text of the abstract on a new line without indentation, using
the entire width (4 5/8”) of the box.
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Blood-Brain Barrier Disruption and Hydrogen Peroxide
Localization in Optic Neuritis: A Correlative MRI and
TEM Study
E. Ann Ellis1, John R. Guy1, Barbara Beck2 and Jeffrey R.
Fitzsimmons2
Depts. of Ophthalmology1 and Radiology2, College of Medicine, Univ. of Florida, Gainesville, FL 32610
Optic neuritis, a demylinating auto-immune disease (DAD) and
usually the initial presentation of multiple sclerosis (MS), is studied
in the animal model, experimental allergic encephalomyelitis
(EAE). Damage to the blood brain barrier (BBB) and free radical
derived oxidants, including hydrogen peroxide (H2O2), have been
implicated in the pathology of EAE and other inflammatory diseases. Disruption of the BBB is monitored clinically in human IF
cases by YOU magnetic resonance imaging (MRI) of gadoliniumDPTA (Gd-DTPA) leakage. READ optic nerves of guinea pigs
(ONGP) sensitized to EAE were THIS imaged by MRI (without
and with Gd-DTPA) prior to euthanasia which was followed by cytochemical SEND localization of H2O2 by the cerium NADH oxidase method. MRI showed no Gd-DTPA leakage in EAE optic
nerves. H2O2 localized in the BOB lumen, cell junctions, basement membrane and perivascular areas of PRICE endothelial cells
of the subarachnoid space, choroid, optic nerve head, retrobulbar
areas, the big round thingamabobs, and on the E-MAIL plasmalemma of inflammatory cells of EAE optic nerves but not in normal optic nerves. The time course and sites of Gd-DTPA leakage
are similar to those of cerium deposition and suggest that H2O2
plays a role in the pathology of BBB disruption in GP EAE.
(Supported by a grant from the National Multiple Sclerosis Society,
New York, NY and in part by a departmental grant from Research
to Prevent Blindness, Inc. New York, NY).
Check the appropriate boxes: [ ] Platform presentation
Projection equipment: [ ] 2x2 slides
[ ] overheads
[ ] Poster (4’ x 8’ space)
[ ]Powerpoint presentation (Windows format only)
Check with The Local Arrangements Committee if other presentation needs are required.
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Historical Perspective
Edited by Jay Jerome
This is another in a recurring feature of the Beam dealing with our history, membership, and the Society’s contributions to science. I hope every SEMS member (young and old) will consider contributing to this feature. I see this as a
space to share our history, get to know each other better, and record the personal side of science. The submission
does not have to be long, just interesting. Send submissions to me at [email protected].
What Value is Membership?
A personal reflection by Jay Jerome.
The pace of science has certainly accelerated in my life time and, like most of you, these days there are ever more
scientific activities competing for my time. In order to set priorities, I must regularly ask myself, “What is the personal value of an activity?” This is true of my membership in SEMS and my attendance at the annual meeting. I
thought it worth sharing with the membership, in the context of the Historical Perspective section of the BEAM, my
reasoning why SEMS membership and meeting attendance remains a priority. The one thing that still stands out in
my memory of the first SEMS (at that time SEEMS) meeting I attended was in 1988 in Athens, GA. Charles Mims
chaired a roundtable discussion on EM in Agriculture. I am not a botanist but I learned a great deal at that session.
Who wouldn’t when the panel included Henry Aldrich, Donald Bechtel, Patrick Echlin, Hilton Mollenhauer, and
Russ Steere? I thought this was an incredible line up for a “local meeting.” As impressed as I was with this session,
this paled by comparison to the great papers presented by students at the Ruska session. That is when I knew I would
be a long time SEMS member. So, the number one reason I attend the SEMS meeting is because of the great science.
An added benefit of attendance, however, has been the great scientific acquaintances that have come my way. As a
lover of history, I was privileged to get to talk extensively with Ivan Roth, Gene Michaels, and Ben Spurlock about
their early experiences in microscopy. SEMS members are great people as well as great scientists and I have made
many close friendships among the SEMS membership. These friendships have been both personally and scientifically
rewarding. Being able to rely on the extensive knowledge and experience of Johnny Carson regarding ciliary dysfunction allowed me to add cilia analysis as a service in the clinical EM laboratory when I was at Wake Forest University. That is only one example of how my participation in SEMS and our meeting have had a strong practical influence on my growth as a scientist. The ability to pick up the phone or send an email to a colleague who I know and
trust for advice when a problem arises in the laboratory is invaluable. The ability to do the same with a vendor of
scientific instrumentation to find out about new equipment or help with a stubborn piece of old equipment is equally
critical. There is a big difference between calling a stranger and calling someone you know and trust. So, when I review my priorities each year, renewing my SEMS membership and setting aside funds to attend the SEMS meeting
are close to the top of my list. Oh yes……….it helps that the SEMS meeting is a H*&@ of a lot of fun!
I will end with the following from the 1979 SEMS meeting.
(sung to the tune “I Could Have Danced all Night.”)
I Could Have Scanned All Nite
Words by Edith McRae, 1979
I could have scanned all Night
I could have scanned all Night,
And still have looked some more.
Yes there were Leukocytes
And there were Phagocytes and
Sights I must explore.
I’ll never know what made it so exciting,
When all at once the scan was right,
I only know ten K-V had made a beam for me
I could have scanned scanned scanned
All night.
I could have scanned all Night
I could have scanned all Night
And still had more to see.
There were some cilia
And microvillia so vivid in 3-D
I’ll never know
What made it so exciting,
When all at once the focus was right,
I only know ten K-V had made a beam for me
I could have scanned scanned scanned
All Nite, All Nite.
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RUSKA AWARD STUDENT COMPETITION
SEMS announces the 2008 Ruska Award Student Competition
The Ruska Award was named in honor of Ernst Ruska an inventor of the electron microscope, and his brother
Helmut, who collaborated in the development of this powerful research instrument. The award recognizes excellence in the biological and physical sciences by student researchers using microscopy as the primary research tool.
To qualify for this award students must 1) submit a written research summary – follow directions listed below 2)
submit an abstract – follow directions in “Call for Papers” 3) and present their work in the form of a PowerPoint
presentation at the SEMS meeting. The presentation should be twelve minutes in length followed by a three minute
question and answer period. The Ruska Award Committee will evaluate the scientific merit of the abstract, the
quality of the writing and micrographs and the oral presentation.
Participants receive free meeting registration and free housing at the meeting hotel (applicants must be willing to
share a room with other students) and a free banquet ticket. The first place winner will be awarded the Ruska
Award plaque and $200.00 at the meeting banquet. All participants receive a certificate of participation.
Ruska Award Guidelines
1. Applicants must be, or have been, an undergraduate or graduate student during the academic year of the meeting. The applicant cannot be a previous Ruska Award winner.
2. Students must present original research conducted by the student. The research may be co-authored by the student’s advisor.
3. Applicants must submit a Research Summary. The written portion of the research summary should not exceed
two pages. An additional page of micrographs or tables /charts related to the project should be included with a
figure legend that describes each micrograph or table. The Research Summary should be submitted in
the Arial font.
4. The Research Summary must consist of:
Title
Author (s) name, and affiliation
Introduction
Methods and Materials
Results/Discussion
References
A plate of micrographs or a combination of micrographs and tables must be accompanied by a figure legend that
describes each micrograph or table.
The entire Research Summary should not exceed four pages and should be submitted using the
Arial font.
5. The Research Summary must be submitted via e-mail to the Ruska Award Chair by Tuesday, April 8,
2008. A signed copy of the Ruska Award Checklist (next page) should be FAXed to the Ruska Chair by the
deadline at (404)727-7760. The student’s advisor must sign the Ruska Award Checklist.
Ruska Award Chair:
Ms. Jeannette Taylor
803 Inland Way
Lilburn, GA 30047
404.712.8674 (office)
404.727.7760 (FAX)
[email protected]
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6. Write an Abstract following the guidelines in the “Call for Papers” article included elsewhere in this issue of The
Beam. An electronic submission of the Abstract clearly labeled “Ruska Participant” must be submitted
to the Program Chair and Co-Chair by Tuesday, April 8, 2008.
Program Chair:
Co-Chair:
Dr. John Shields
Dr. Charles Humphrey
CAUR
Centers for Disease Control
151 Barrow Hall
1600 Clifton Rd
University of Georgia
CDC DVRD MS G32
Athens, GA 30602
Atlanta, GA 30333
Ruska Award Checklist: Mail this form with your Research Summary
1. Student’s name_____________________________________________.
2. Your affiliation _____________________________________________.
3. Your address_______________________________________________.
4. Research Summary includes:
Title, Authors, Affiliation___.
Introduction____.
Methods & Materials____.
Results____.
Discussion____.
Figures &Legends____.
Send Four Copies to Ruska Award Chair and One copy to Program Chair.
5. Sponsor verification:
“I verify that the above listed Ruska Award participant is an undergraduate/graduate student during this academic
year.”
Signed/Dated____________________________________________________________.
Ernst Ruska (right) and Max Knoll circa
1931.
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Ruska Award Method of Evaluation
The Ruska Award Committee will evaluate the research summary and oral presentation using the following criteria:
I. Submitted Text
A. Scientific Merit
1. Soundness of research proposal
2. Experimental design and thoroughness of investigation (originality, correlated techniques)
3. Validation of conclusions
4. Application of microscopy and associated methods to answer experimental questions
B. Micrographs
1. Presentation of specimen (fixation, quality of sectioning and staining)
2. Instrumentation
3. Photographic quality (aesthetics, density, contrast)
C. Quality of Writing
1. Organization
2. Clarity
3. Grammatical correctness
II. Oral Presentation
A. Clarity
B. Communication
C. Quality of Presentation
D. Response to audience questions
E. Adherence to presentation time limit (12 minutes for the presentation and 3 minutes for questions)
Microscopy Society of America
Application for Membership
230 East Ohio Street, Suite 400
Chicago, IL 60611-3265
800.538.3672, 312.644.1527, Fax: 312.644.8557
Email: [email protected]
To Apply, please go to
http://www.msa.microscopy.org/msaunits/members/newmemberapplication.html
And fill out the online form
Change of affiliation,
phone, e-mail, address?
Contact one of these individuals with your
new information so members can find you!
Cynthia Goldsmith, Secretary
1600 Clifton Road
CDC MS G30
Atlanta, GA 30333
404.639.3306
[email protected]
Karen Kelly, Treasurer
Univ. of Florida
PO Box 110700
Gainesville, FL 32611
353.392.1295
[email protected]
David Burk, Editor
CBB Core
PBRC
6400 Perkins Road
Baton Rouge, LA 70808
225.763.2779
[email protected]
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Application form for travel support for
technologists attending sems 2008
Each year the SEMS Council considers applications for travel awards to support meeting attendance by Technologists.
Awards are made on a competitive basis from limited funds designated by the society for this purpose.
Submitting this application does not guarantee that an award will be made.
A. Please Print or Type:
Name:_____________________________________________________________________________
Job Title:___________________________________________________________________________
Company or Institution:_________________________________________________________________
Mailing Address:______________________________________________________________________
City, State, Zip:_______________________________________________________________________
Phone:______________________________________________________________________________
E-mail:_____________________________________________________________________________
SEMS member for ______ years.
Name of immediate supervisor:____________________________________________________________
Supervisor’s Phone:____________________________________________________________________
Supervisor e-mail:_____________________________________________________________________
Signature of Supervisor:_________________________________________________________________
(indicating support for your attendance)
B. Dollar amount of local funds available for you to attend SEMS 2008:
Minimum amount requested from SEMS to attend meeting: (fill all that apply)
Hotel
__________________________
Registration (available only to those presenting)
__________________________
Automobile travel (calculate $0.30/mile)
__________________________
Air Travel (awards for air fare will receive
__________________________
second priority, may not be sufficient to cover
entire fare, and are subject to final
confirmation of cost)
Total:
__________________________
It is assumed that if this minimum cannot be met, you will be unable to attend the meetings. More funds than your requested
minimum may be allocated after consultation with you, the Executive Council, and other applicants.
*Please use a separate sheet to briefly comment on how participation at the meeting would benefit you and
enhance your present position.
Applications must be received by March 1, 2008. Mail to:
Robert Simmons
Department of Biology
Ga State University
Atlanta, GA 30303
404.651.3138
[email protected]
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Southeastern Microscopy Society
Membership Application
The purpose of SEMS is to increase and spread knowledge of microscopy. Most members
share an interest in the biological and physical sciences, applications of light microscopy, and transmission and scanning electron microscopy, and microscopical analysis. The annual meeting presents
invited speakers, contributed papers and posters, as well as commercial exhibits of light and electron microscopes and related equipment and supplies.
Annual dues for regular members are $20, students (with signature of faculty advisor) $10
and corporate members $100 for the calendar year.
Name:__________________________________________________________________
Company or Institution:_____________________________________________________
Mailing Address:___________________________________________________________
City, State, Zip:____________________________________________________________
Telephone:____________________________ E-mail:_____________________________
Fax:_________________________________ URL:______________________________
Membeship:
Regular
Student
Corporate
*Signature of Faculty Advisor:__________________________________________________
Make checks payable to SEMS and mail to:
Karen Kelly, Treasurer
PO Box 118525
University of Florida
Gainesville, FL 32611
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Attention Corporate Members
Order Form for Advertisements in The Beam
Please fill out the form below and
send your advertisements in
TIFF or PDF format to
[email protected] and a
check payable to “SEMS
Newsletter” to:
Enclosed is a disk with digital advertising insert in TIFF
or PDF format.
Full page ($100/issue)
Half page ($50/issue)
For convenience, you may also send TIFF or PDF files to
the editor via e-mail at [email protected]
David H. Burk, Editor
481 Brookhaven Dr.
Baton Rouge, LA 70808
Company Name:________________________________________________
Represented by:________________________________________________
Mailing Address:________________________________________________
City, State, Zip:________________________________________________
Phone:________________________________
FAX:_________________________________
E-mail:________________________________
URL:_________________________________
Amount of Order:_______________________
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