Caddy 4000 Plus

MegaBACE™ Automation Systems
Caddy 4000 Plus
Selling Support Pack
(version 1.0)
Watrex Praha, Ltd.
All rights Reserved
MegaBACE 4000 384-capillary system
MegaBACE™ is a registered trademark of Amersham Biosciences
MegaBACE 4000 384-capillary system
MegaBACE™ is a registered trademark of Amersham Biosciences
The Scientist 16[6]:41, Mar. 18, 2002
The Core of DNA Sequencing
Thanks to centralized core facilities, "drive-through" convenience
has replaced the grueling days of manual sequencing
By Jessica M. Pisano
“For really high-throughput needs, the newly introduced
MegaBACE 4000 can process 384 samples in one run.”
“[With ABI's PRISM® systems] .. samples can be drawn
from 96- or 384-well plates, allowing multiple runs to take
place unattended over a 24-hour period..”
“Another consideration [to purchase a new automated
DNA sequencer] is the machine's level of automation.
Harvard's Vannberg says that his facility wanted
Instruments that could be operated 24 hours a day,
mostly unattended.”
Caddy 4000 Plus
– basic facts
• complete automation of MB 4000
• compatible with ICM 3.0 and up
• cathode + anode module
• reduced evaporation
• 18 plate positions (384 wellplates)
• walkaway automation
• 2 tank positions
• workflow flexibility
• 156 tube positions
• barcoding capability
Cathode Automation Module
(replacing plates and tanks at the cathode)
Plate base
Plate hotel with 20 drawers
Accurate pneumatic gripper
Caddy 4000 Plus (Cathode Automation Module)
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Plate hotel: capacity 18 plates + 2 tanks
Dimensions 460x320x900 mm (WxLxH)
Weight 68 kg
Power requirements 115/230 V AC
50/60 Hz 160 VA
Anode Automation Module
(replacing tubes at the anode)
Tube rack
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•
•
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156 tubes capacity
13 columns (1-13)
12 rows (A-L)
Random access
Pingle (temporary storage)
OLD SET
NEW SET
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Two-pack capacity
Close to anode tray
Direct manual access
Error recovery
Two-step transfer
Step 2 (fast)
Pingle
ep
St
MegaBACE
w)
o
l
s
1(
Anode slider
Tube rack
Gripper
Caddy 4000 Plus (Anode Automation Module)
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Rack: capacity 156 tubes (26 x 6-packs)
Pingle: 12 position temporary storage
Dimensions 480x430x650 mm (WxLxH)
Weight 35 kg
Power requirements 115/230 V AC
50/60 Hz 160 VA
Flexibility
Customized definitions of workflows
- multiple injections
- user-defined protocols
Customized definitions of containers
- buffer tank
- buffer tubes
- water tubes
- empty tubes
Automated protocols
Flush and Dry
Capillary Focusing
Available throughput configurations*
Basic configuration:
24 hours unattended operation (9 runs)
- 9 sample plates
- 9 buffer plates
- 1 water tank
Extended configuration
32 hours unattended operation (12 runs)
-12 sample plates
-6 buffer plates
-1 water tank
Ultimate configuration
48 hours** operation (18 runs)
-18 sample plates
-2 buffer tanks
*) assuming 160 minutes total turnaround time
**) increased sample volumes required to reduce evaporation
SCOUT automation software
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Flexibility (user profiles)
•
Active communication with MegaBACE
•
Integration with ICM
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Barcoding capability
•
Remote notification
SCOUT – cathode view
SCOUT – anode view
SCOUT – mapping concept
SCOUT – support for manual protocols
Sample evaporation
(384 wellplate)
corner wells
outer rows
s econd rows
inner wells
11
Volume [µ L]
10
9
8
7
6
5
0
2
4
6
Time [hours ]
8
10
OVERVIEW OF CADDY AUTOMATION SYSTEMS:
MB - MegaBACE, CD - Caddy, ABI - Applied Biosystems
MegaBACE™ is a registered trademark of Amersham Biosciences
Things change …
Me gaBACE au t o m at io n we bs it e :
www.Me gaBACE.n e t
-
n e ws an d u pdat e s
FAQ
ph o t o galle ry
m ark e t in g an d s ale s
s e rvic e an d s u ppo rt
u s e r dis c u s s io n s
MegaBACE™ is a registered trademark of Amersham Biosciences