Fullerene, Super Anti-Oxidant - in

Fullerene, Super Anti-Oxidant
-Clinical Test Results on Whitening and
Anti-Aging-
Vitamin C60 Bioresearch Corporation
Tomoko OHTSU
August 30th ,
2013
Minimum Knowledge about Fullerene!
1. Fullerene(C60) is a carbon allotrope of carbon, like a diamond.
2. Fullerene(C60) is a Nobel Prize winning material
3. Fullerene(C60) is a super anti-oxidant.
- 172 times as effective as Vitamin C
- stable against light and/or heat
- power is long-lasting (more than 24 hours)
2
Great Effects of Fullerene
Anti-aging（preventive effects of cell death)
Whitening
（Melanin Control）
Inflammation Easing
(reducing of a ruddy face)
Sunscreen / UV cut
Pore Tightening
Recover
Skin barrier
Anti-Wrinkle
Sebum Oxidation Control
3
Data of Fullerene Effect
- in vitro Study1. Free Radical Scavenging activity
2. Comparison data with
other anti-oxidant
ingredients
3. Preventive Effect of Fullerene
4
Free Radical Scavenging activity
Concentration of Free
Radical
high
low
Without Fullerene
With Fullerene
5
Radical scavenging activity of Fullerene
Antioxidant Activity (AOA%)
100
80
60
40
20
0
Linoleic acid
H2O2
UV ray
-20
Vitamin E
Vitamin C Derivative(APS)
Fullerene(Radical Sponge)
6
0
a-lipoic acid
APPS
17.7
crucmin
quercetin
20
coenzyme Q10
isoflavone
catechin
vitamin E
PVP/C60On
40
PVP/C60O
PVP/C70
PVP/C60
Activity(AOA%)
Antioxidant
Activity(AOA%)
Antioxidant
Comparative testing with other antioxidant
ingredients by β-Catotene method
60
49.5
35.6
29.1
25.1
14.8
7.35
7
Preventive effect of Fullerene
With
11 hours later
Without Fullerene
Fullerene
8
Clinical Test Results by using Fullerene
contained cosmetics
1. Brightening (Whitening) Effects
(Radical Sponge™ )
2. Effect of Fullerene on the Skin Barrier
(Radical Sponge™)
3. Fullerene is effective against wrinkle
( LipoFullerene™ )
9
Brightening (Whitening) Effects
by Fullerene
Effectively eliminate UV-induced free
radicals
Prevent excessive melanin production
Verified by various clinical trials
10
Suppression of Melanin production by Fullerene
Without Fullerene
With Fullerene
Let’s check the movie!!
11
Brightening (Whitening) Effect by Fullerene
Subjects: 18 females aged 34 to 49 Area: Face
The melanin index decreased intentionally on the 4 weeks.
p < 0.001
1.3
1.2
p < 0.001
p < 0.001
1.5
1.0
Hemoglobin Index
Melanin Index
1.1
0.9
0.8
0.7
0.6
1
0.5
0
0
4
8 (weeks）
0
4
8
（weeks）
You can feel the Whitening/Brightening effect in about 1-2 month.
Continuous use of Fullerene contained product is the key to have better
13
results.
Brightening (Whitening) Effect by Fullerene
After 3 month
After 5 month
Continuous use of Fullerene contained product will lead you
more brighter and fair skin condition.
14
Effect of Fullerene on the Skin Barrier
by Fullerene
Increase “Transglutaminase 1” mRNA
expression.
Increase “Cornified Emvelope” formation and
help maintain Skin barrier function.
Clinical study: Help in recovering skin barrier
15
function.
Structure and function of the stratum corneum
Cornified
envelope
（CE）
Moisture loss
Intercellular lipid
Stratum
corneum
20 μm
Cornified
cell
Granular layer
Normal stratum corneum
dry
skin
dullnes
s
rough
skin
・drying
・UV
・stress
・aging
flecks
wrinkles
Stratum corneum with decreased
skin barrier function
16
Clinical Test :
Tape Stripping Method to measure Trans Epidermal Water
Loss (TEWL)
Radical Sponge® helped recovery of TEWL on 2nd and 3rd day
significantly
100
RS(0%
)
Rate of recovery （％）
80
60
RS(1%)
#
#P<0.05 vs. RS(0%) #
40
20
0
days
-20
-40
Trial Design
0
40
1
1
2
3
2
3 study
Randomized
blinded
4
Method
TEWL changes after Tape Stripping
Subjects
10 healthy subjects (5male 5female)
Trail period and applications
Solution was applied twice daily for 5days
17
Fullerene is effective against wrinkle
by Fullerene
Remove the Free Radicals which is the
primary cause of wrinkles.
Skin structure is improved steadily.
Fullerene/squalene cream significantly
reduced wrinkle area compared to the
placebo-treated area.
18
Wrinkles formation by ultraviolet rays
Decrease of MMP9 and FOS mRNA means repression of wrinkles formati
The active oxygen which occurred by ultraviolet rays activates FOS
(intracellular signaling) and MMP9 (degradative enzyme of collagen),
and forms wrinkles.
UVB
Wrinkle
formation
RO
S
Epidermi
s
FOS
laminin-5
Basement
membrane
IV type collagen
Dermis
VII type collagen
MMP9（Matrix metallopeptidase 9）・・・ degradative enzyme of laminin-5 etc.
19
Fullerene is effective against wrinkle
by LipoFullerene
Guideline
s
Guidelines for Evaluation of AntiWrinkle Products prepared by the
Japanese Cosmetics Science Society
in 2006
Trial
Design
A randomized, double-blind test
Trial
Period
Eight weeks (September 24, 2008 ～
November 19th, 2008）
Subjects
23 Japanese women in their 30s to
40s with wrinkle grade 2 to 3.
Test
Samples
1%-LipoFullerene-blended cream
(active) and Lipofullerene free cream
(placebo)
Evaluation
Shadow image
Skin surface replica
10 x 10 mm
Wrinkle:
few
many
The percentage of wrinkle area decreases in the
LipoFullerene application group than a Placebo application
20
Great Effects of Fullerene
Anti-aging（preventive effects of cell death)
Whitening
（Melanin Control）
Inflammation Easing
(reducing of a ruddy face)
Sunscreen / UV cut
Pores Tighten
Moisturizer
(Skin barrier)
Anti-Wrinkle
Sebum Oxidation Control
21
For Basic Skin Care Products and MORE!
Basic Skin Care Products
- Lotion
- Essence/Serum
- Cream
+
1. Color Cosmetics
(BB Cream, CC Cream)
2. Hair care
(Scalp tonic,
Shampoo & Treatment)
3. Beauty Laser care
4. Sun screen
5. Facial Mask
22
Fullerene Products in
Overseas
Anti-oxidant Whitening Serum
With Fullerene RS™
23
Fullerene is famous for Doctor’s Cosmetics
More than 1,500 clinics in Japan
has selected our Fullerene cosmetic
ingredients!!
1600
Number of Clinics
1400
1200
1000
800
600
400
200
0
2005
2007
2009
2011
2013
24
Fullerene Contained Products
St.Clare
（Taiwan）
TUNEMAKERS
WithUs
アプロ
サイエンス
Dr.WU
（Taiwan）
Unicity
アジュバン
コスメ
Dr. Recella
MISSHA
（Korea）
Felice Towako
Cosmetics
AR
Over 1000 items
are selling in the
market !!
JMEC
2009
Launch LipoFullerene
DHC
2008
1000 Clinics selected Fullerene
Dr.Ci labo
Doctor Products
マードゥレクス
2011
CBON
Dr.Perricone
（USA）
ISA Nox
(Korea）
NOVA C60
（Canada）
Dr.Brandt
（USA）
Oil-Soluble
Fullerene
2007 Entered to Internet Market
2006 Start Selling in Overseas（Korea・Taiwan・USA）
Derfarma
For Clinical
Doctors
2005 Launch Radial Sponge
Water-Soluble
Fullerene
25
Our Products
Radical Sponge
(water-soluble)
LipoFullerene
(oil-soluble)
26
Trademark (LOGO)
Products which containing more than 1% of our ingredients(Radical Sponge
or LipoFullerene) can put our trademark to their product package and web p
27
Marketing Points of Fullerene!!
Nobel Prized Winning Ingredient
172 times as effective as Vitamin C
Stable against light(UV) and/or heat
Power is long-lasting (more than 24 hours)
Abundance of Clinical data
Multifunctional ingredient
Safety and secure ingredient selected by Clinic
Doctors
28
Samples of our ingredient is available!!
After concluding Non-Disclosure Agreement (Sample
Evaluation Agreement), VC60 will send sample to the
customer for free.
• Radical Sponge (water-soluble Fullerene) : 20 g
• LipoFullerene (oil-soluble Fullerene): 16 g
29
Sample formulations are available!!
• Radical Sponge (water-soluble Fullerene)
Serum & Cream
• LipoFullerene (oil-soluble Fullerene):
16 g
Milk Lotion W/O type & O/W type
30
Exhibitions
in-cosmetics Asia 2013
Date: October 29th – 31st, 2013
Time: 10:00 – 17:00
Place: BITEC Hall Bangkok,
Thailand
Booth number: E10
(Booth share with Bronson &
Jacobs)
31
Thank you for your attention!
Vitamin C60 BioResarch Corporation
Contact:
[email protected]
+81-3-3517-3252
http://www.vc60.com