TRAINING MODULE FOR LAB TECHNICIANS BLOOD

TRAINING MODULE FOR LAB TECHNICIANS
BLOOD CELL COUNTER
HORIBA Medical
MICROS ES60
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CONTENTS:
1. SAMPLE COLLECTION……………………………………………………..03
2. OPERATIONAL CONDITION……………………………………………….04
2.1 LOCATION………………………………………………………………..04
2.2 GROUNDING……………………………………………………………..04
2.3 HUMIDITY AND TEMPRATURE CONDITION…………….………….04
2.4 ELECTROMAGNETIC ENVIROMENT CHECK……………….……….04
2.5 MAIN POWER SUPPLY………………………………………………….04
3. SYSTEM OVERVIEW…………………………………………….………….05
3.1 SOFTWARE OVERVIEW…………………………………….…………..05
3.2 SOFTWARE BUTTON DESCRIPTION …………………….…………...05
4. STANDERD OPERATING PROCEDURE……………………….………….08
5. MAINTENANCE…………………………………………………..…………10
5.1 REAGENT REPLACEMENT………………………………..……………10
5.2 PAPER REPLACEMENT…………………………………..……………..11
5.3 CONCENTRATED CLEANING………………………………………….12
6. ALARMS AND FLAGS………………………………………………………14
6.1 SYSTEM ALARMS……………………………………………………….14
6.2 REAGENT ALARMS……………………………………………………...15
6.3 FLAGS……………………………………………………………………..17
7. CONTACT DETAILS…………………………………………………………18
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1. Sample Collection:
Every specimen must be accompanied by a request slip, which should indicate date, patient’s full
name, age and sex, sample Id, provisional diagnosis, , exact time the sample was obtained and
the name of the person who collected the specimen with signature. It is necessary that the request
slip be complete and clearly written. Incomplete information may lead to total loss of the report.
Samples should be processed as early as possible. Temporary storage in refrigerators is advisable
except for C.S.F. and specimens submitted for bacteriological cultures. Urine and other body
fluid should be processed as early as possible (Before 2 hrs) as delay causes disintegration of
cells and loss of morphology.
Blood Sampling:
Blood is one of the most common samples studied in laboratories. Blood will clot within few
minutes after it is removed from the body unless an anticoagulant is used.
Whole Blood:- Blood with anticoagulant is called whole blood.
Plasma :- Fluid portion of unclotted blood is called plasma and is obtained from anticoagulated
blood.
Serum:- Fluid portion of clotted blood and is obtained from clotted blood, which is collected
without any added anticoagulant.
ANTICOAGULANT:The anticoagulant prevents the blood from clotting. Most of anticoagulants remove calcium.
Which is one of factor required for coagulation.
EDTA – Ethylene Diamine Tetra Acetic Acid –
Sodium & Potassium salts of EDTA are powerful anticoagulants specially suitable for Routine
haematological work including cell counts and blood film staining, It is used for Hb, TLC, DLC,
CBC, PLT Count, Reticulocyte count.
Vaccutainers:- These are plastic tubes with coloured cap with capacity of 3-5ml blood used in
laboratory now a days. These may be with or without vacuum. The color of the cap indicates the
presence of anticoagulant present inside the tubes. The label is attached with the tube for
patient’s identification.
RED Cap: No chemical for serum separation (Biochemistry and Serological test)
PURPLE Cap:- Potassium EDTA for CBC, Hb, TLC, DLC, PBF, Reticulocyte count, TEC
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2. Operational Conditions
*The operation of the ABX Micros ES 60 should be restricted to indoor location use only.
2.1 Location
■ Place your instrument on a clean and leveled table or workbench. Keep in mind that the
instrument weighs approximately 17 Kgs.
■ Avoid exposure to sunlight.
■ Place your instrument where it is not exposed to water or vapor.
■ Place your instrument where it is free from vibration or shock.
■ Provide a space of at least 20 cm (8 in.) at the back of the instrument for a proper ventilation
and an easy access to connections.
■The Power switch and Power supply connection should always be accessible. When positioning
the system for operational use, leave the required amount of space for easy accessibility to these
items.
2.2. Grounding
Proper grounding is required when installing the system. Check the wall outlet ground (earth) for
proper grounding to the facilities electrical ground. If you are unsure of the outlet grounding,
contact your facilities engineer to verify the proper outlet ground.
2.3. Humidity and Temperature Conditions
Instrument operating temperature: from +16°C (+61°F) to +30°C (+86°F), with a relative
humidity of 85% maximum, without condensation. If the instrument is stored at a temperature
lower than 10°C (50°F), it should stand for one hour at a normal room temperature before use.
2.4. Electromagnetic Environment Check
The instrument has been designed to produce less than the accepted level of electromagnetic
interference in order to operate in conformity with its destination, allowing the correct operation
of other instruments also in conformity with their destination.
In case of suspected electromagnetic noise, check that the instrument has not been placed in the
proximity of electromagnetic fields or short wave emissions, e.g. Radar, X-rays, Scanners, Cell
phones, etc.
2.5. Main Power Supply
Grounding is required. Check that earth wall-plug is correctly connected to the laboratory
groundingSystem. If there is no such system, a ground stake should be used. Use only main
supply cable delivered with the instrument.
Main power supply voltage fluctuations must not exceed +/- 10% of the nominal voltage.
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3. System Overview
3.1 Software Overview
A software application is installed on the ABX Micros ES 60.
The main screen includes the following items:
1 = Menu access buttons: to access the main functions of the system (sample identification,
quality control, results history, maintenance).
2 = Exit button: to access the Exit screen (operator change, instrument restart, instrument
shutdown).
3 = Status button: to display the Status screen which indicates any problem on QC, reagents,
Archives, application or instrument and gives the corrective actions to perform.
4 = Contextual toolbar: to access the functionalities related to the screen currently displayed.
3.2 Software Buttons Description
Menu Access buttons
The menu access buttons are located on the right-hand side of the screen. These buttons allow
the user to access the main functions of the system.
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Sample Identification
To enter the patient's sample or control information and to launch analyses.
Quality control
To monitor a set of three QC tests.To display QC results, LJ graphs and XB statistics.To manage
instrument calibration.
Result history To display all stored patient results, from the current or
archived sessions.
Maintenance
To perform maintenance operations (cleaning cycles, reagent management, troubleshooting, etc).
To edit/to modify several parameters of the instrument (certain parameters modification may
require a specific user level).
Press a menu button to display the corresponding menu (default menu is Sample Identification).
The yellow edging of the active menu button shows the user in which menu he is located.
Exit Button and Exit Screen Description
The Exit button is located at the bottom left corner of the screen.
Exit button To open the Exit screen.
The Exit screen allows three possible actions to perform.
Log out To disconnect the current user and to display the Login window.
Restart To restart the instrument.
Shutdown To shutdown the instrument.
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Status Button Description
The Status button is located at the top-right corner of the screen. It allows to display the Status
screen which informs the user on the general system state, lockings and the actions to perform to
unlock the instrument:
Green status The instrument is running correctly.
Orange status An alarm is raised but does not lock the instrument. An analysis can be run.
Red status An alarm is raised and locks the instrument. Analyses cannot be run except QC.
The Status button blinks when a new alarm is raised: the Status screen has then to be checked.
Contextual Toolbar Description
Depending on the screen currently displayed, buttons of the contextual toolbar may change.
Buttons listed below are the most commonly displayed:
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5. Maintenance & troubleshooting
5.1 Reagents Replacement
Access: Main screen > Maintenance menu > Reag. (tab)
An reagent alarm is triggered and display in the Status screen if:
■ the reagent is expired,
■ the remaining number of tests is lower than 10,
■ the diluent container is empty.
Then, the ABX Minidil LMG must be changed
1. Unscrew the stopper assembly of the new container using the opening tool provided with your
Instrument
2. Remove the stopper assembly straw from the empty container and insert it into the new one.
3. In the Reag. tab, select the DILUENT radio button.
4.Press the Replace button.
5. Press Validate in the dialog box.
6. In the Edit reagent screen, press Edit in the contextual toolbar.
7. Press the Lot field to activate it.
8. Read the diluent barcode label with the barcode reader or type the lot number using the virtual
keyboard or an external keyboard (if one is connected). The instrument searches diluent
information in the reagent database.
9. When diluent information are displayed, press Validate in the contextual toolbar.
10. In the Reag. tab, press the Prime button to launch a prime cycle.
To Replace a Reagent Bottle
The Lyse reagent is provided in a bottle (ABX Minilyse LMG or ABX Alphalyse) or in a small
pack (ABX Alphalyse 360):
■ To replace the Lyse bottle: follow the procedure below.
■ To replace the Lyse small pack: refer to the To Replace the ABX Alphalyse 360 pack
(Optional) chapter.
1. Open the reagent compartment flap cover.
2. Remove the empty bottle from the reagent compartment.
3. Install the reagent straw and the bottle stopper on the bottle.
4. Connect the tube to the reagent bottle.
■ For the Cleaning bottle: connect the blue tube.
■ For the Lyse bottle: connect the white tube.
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5. Place the reagent bottle in the reagent compartment
6.
Close the reagent compartment flap cover.
7. Run the priming of the reagents.
5.2 To Replace the Integrated Ticket Printer Paper
1. Lift the lever
2. Lift the printer cover.
3. Remove the old paper roll.
4. Load the new paper roll:
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5. Close the printer cover by pressing simultaneously on both ends of the cover. A "click "
should be heard.
5.3 Concentrated Cleaning
IT IS RECOMMENDED THAT CONCENTRATED CLEANING SHOULD BE PERFORM IN EVERY 15 DAYS
Access: Main screen > Maintenance menu > Serv. (tab) > Customer services (button) >
Hydrau. (tab)
Prepare a 5 mL syringe, a bottle brush and ABX Minoclair or bleach solution containing 12%
active
chlorine.
1. Press the Concentrated cleaning radio button.
2. Press the Start cycle button and follow the steps that appears on the screen.
This message is displayed: "This cycle takes 10 minutes; it cannot be cancelled until it has
finished; are you sure that you want to run this cycle? If so, open the front door and press
confirm
to continue.".
3. Open the front cover.
4. Press Validate in the dialog box.
This message is displayed: "Put 3 mL of MINOCLAIR in WBC chamber and validate to
continue.".
5. Dispense the bleach solution into the WBC chamber (or into the hole on the top of the cover
that it
goes into the chamber).
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6. Press Validate in the dialog box.
This message is displayed: "Put 3 mL of MINOCLAIR in RBC chamber and validate to
Continue.".
7. Dispense the bleach solution into the RBC chamber (or into the hole on the top of the cover
that it goes into the chamber).
8.
Press Validate in the dialog box.
This message is displayed: "Close the front cover and validate to continue.".
9. Close the front cover.
10. Press Validate in the dialog box.
The cleaning cycle begins and a cleaning time is displayed. This concentrated cleaning cycle
involves different cycles, backflush, aspiration, rinsing, which allow a good cleaning of the
chambers and apertures.
11. When the procedure is complete, perform a startup cycle to check that the reference blank
parameters are within their limits.
a. Press the Status button to open the Status screen.
b. Press the Startup button.
12. Rerun quality control and check that the results are within the assay limits listed on the
control
assay sheet that comes with the controls
Once the startup cycle has passed, sample analysis may begin.
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6. ALARMS AND FLAGS
6.1 System Alarms
QC alarms
QC status button
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6.2 Reagent alarms
Instrument status button
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6.3 Flags:
« $ » mean: the system did 3 counts rather than 2 for the concerned
parameter.
The results can be reported, but the parameter should be monitored for
the next sample Analyzed.
« * » mean : "Reject" = the system did 3 counts. All 3 counts were
outside the system precision limits for that specific parameter. The
result is rejected . The results should be verified by re-running the
sample.
Suspicion is noted « ! » mean the Hemoglobin blanck of the measured
is too much différent from reference blanck done at the startup. This
result can be reported, but Hgb should be monitored for next sample
-.-- No result available.
« D » result exceed linearity limit
« H » mean result is Higher than normal range.
« L » mean result is Lower than normal range.
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7. Contact Details for Horiba India Pvt. Ltd.
3 Part cell counter (HORIBA India Private Limited)
Mr. Manish Agrawal
9314686396
Jaipur
Mr. Naresh Rohilla
9636521879
Mr. Vibhav Kumar
9582506134
Mr. Vibhav Kumar
Ajmer
9582506134
Mr. Vaibhav
Kota
9654073503
Mr. Sunil Parekh
Jodhpur
9828031552
Mr. Vaidhant Kumar
Bhartpur
8010437906
Mr. Hemant Kumar
Udaipur
09024628903
Mr. Ranjeet Kumar
Bikaner
09829709175
RMSC Support: Consultant Biomedical Engineer
Phone: 0141-2225885
Email: [email protected]
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