SPRIworks-HT Brochure (BR-15981)

SPRIworks Systems
Fully automated library construction systems
with built-in size selection and cleanup
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BR-15981B
SPRIworks Technology
Fully automated fragment library construction systems
Choose the Automated Sample Prep System That is Right for Your Lab
History of Trust: Sample Prep for Genomic Sequencing
Low-Medium Throughput (Up to 30 libraries/day)
High Throughput (Up to 96 libraries/day)
SPRIworks Fragment Library Systems
SPRIworks HT System
• Fully automated library construction with built-in
size selection and enzymatic cleanup
• Fully automated library construction, with built-in
size selection and enzymatic cleanup
• Process up to 30 samples per day
• Process up to 96 samples per day
• Bench-top system
• SPRIworks chemistry automated on Biomek
robotic platforms
2010 - Beckman Coulter utilizes SPRI chemistry for next generation sequencing sample preparation
with the launch of SPRIworks Fragment Library Systems.
• Less than 5 minutes hands-on setup time
2005 - Recognizing the value of SPRI chemistry optimized on Biomek automated workstations,
• Prepares samples for Illumina*, Roche* and
Life Technologies* next generation sequencers
• Less than 30 minutes hands-on preparation time
• Prepares samples for Illumina next generation
sequencers
Beckman Coulter acquires Agencourt Bioscience.
• Includes optional methods for qPCR† setup
normalization and pooling
2003 - As a result of the high quality sequencing data produced by Agencourt,
the NHGRI designates Agencourt as one of only five Large-Scale Sequencing Centers
and the only commercial center responsible for genome sequencing of organisms of
high biomedical importance. SPRI technology, robotics and bioinformatics
enable Agencourt to be acknowledged by the sequencing community as
one of the preeminent sequencing facilities.
Beckman Coulter SPRIworks vs Manual Processing Methods
Manual
Up to 8 libraries per day
SPRIworks
Up to 30 libraries per day
SPRIworks HT
Up to 96 libraries per day
DNA Extraction
DNA Extraction
DNA Extraction
Shear DNA
Shear DNA
Shear DNA
End Repair
End Repair
End Repair
2001 - Due to SPRI’s flexibility, ability to produce high quality nucleic
acid, and ease of automation, Agencourt launches sequencing services
and genomics products based on SPRI chemistry.
1995 - Agencourt recognizes the advantages of magnetic bead
technology and acquires the license for SPRI paramagnetic beads.
SPRI is immediately used as the platform for all products and services.
Clean-up
A-Tailing
Clean-up
Ligation
A-Tailing
Clean-up
Ligation
Library construction is the number one bottleneck in the sample
preparation process for next generation sequencing, requiring
significant hands-on time. Additionally, multiplexing capabilities
are putting strain on laboratories to prepare more libraries for
sequencing than ever before.
SPRIworks and SPRIworks HT Fragment Library Systems
eliminate manual library construction and provide a significant
boost to productivity, reproducibility and consistency.
By automating sample processing, laboratories can now maximize
the return on their sequencing investments while freeing up valuable
resources to focus on data analysis and biological interpretation.
Size Selection
Automated
1-30 fully sized
libraries per day
A-Tailing
Clean-up
Ligation
Clean-up
Clean-up
Fully Automated Library Construction
Clean-up
Clean-up
Manual
1-8 fully sized
libraries per day
per person
Size Selection
Clean-up
Automated (optional)
Covaris E-series can be
integrated on Biomek FXP
Automated
1-96 fully sized
libraries per day
Clean-up
Size Selection
Clean-up
Clean-up
PCR Set-up
PCR Set-up
PCR Set-up
PCR
PCR
PCR
PCR Clean-up
PCR Clean-up
PCR Clean-up
qPCR
qPCR
qPCR
Normalization
Normalization
Normalization
Automated (optional)
T-robot on Biomek FXP
Automated
with method suite
Automated
with method suite
Pooling
Pooling
Pooling
Sequencing
Sequencing
Sequencing
SPRIworks Fragment Library Systems I, II and III
For Illumina, Roche and Life Technologies next generation sequencers
Built-in SPRI Size Selection and Cleanup Chemistry
Increase library construction throughput without running gels, cutting bands for DNA fragment size selection
or using columns for cleanup.
Free Your Lab From the Constraints of Next Generation Sequencing Sample Prep
The Beckman Coulter SPRIworks Fragment Library
Systems improve laboratory productivity and liberate
scientists to spend time focusing on data analysis.
Run up to 10 samples at a time
• Maximize sequencing run capacity by multiplexing
up to 10 samples in parallel on each system
No more manual size selection gels, cleanup columns
or manual fragment library construction.
Fully automated library construction process
• Reproducible and consistent library output
SPRI-TE Nucleic Acid Extractor
• Only 5 minutes of hands-on time
Reagents predispensed in a sealed cartridge
200-400 size selection
• Simple library construction process
300-600 size selection
Short and long fragment sizes isolated using SPRIworks Fragment Library System I for Illumina GA Sequencers and analyzed using
Agilent* High Sensitivity DNA Chip.
• No more labor intensive pipetting
Multi-platform Library Construction
Method Card
Not only does the SPRIworks bench-top instrument occupy a small footprint, but it prepares fragment libraries for
Illumina, Roche and Life Technologies next generation sequencers.
Cartridge
SPRIworks Fragment Library Systems have three components; The SPRI-TE Nucleic Acid Extractor, a method card that controls liquid
handling, and a cartridge containing all the reagents required to prepare a single library.
System I for Illumina Sequencers
System II for Roche Sequencers
System III for Life Technologies Sequencers
Throughput
Up to 10 samples in about 5 hours
Up to 10 samples in under 4 hours
Up to 10 samples in under 4 hours
DNA input amount
3-5 µg
500 ng
1 µg (3 µg for 150-250 size selection)
Size selection ranges
• No size selection
• 200-400 bps
• 300-600 bps
• No size selection
• 350+
• 500+
• No size selection
• 150-250 bps
• 150-350 bps
Supported input sample types
Sheared DNA, cDNA, amplicons
Sheared DNA, cDNA, amplicons
Sheared DNA, cDNA, amplicons
Basic Operation Steps
SPRIworks systems are simple and easy to operate. Hands-on time is significantly decreased
so that technical expertise can be allocated to experiment design and data analysis.
Step 1. Load cartridge into rack
Step 2. Transfer input samples
Step 3. Load cartridges, samples, adapters and labware
Step 4. Insert method card, select size range and press START
SPRIworks HT High Throughput System
For Illumina next generation sequencers
Built In SPRI Size Selection and Cleanup
High throughput library construction without running gels,
cutting bands or using columns for cleanup.
Sample Prep Freedom for High Throughput Next Generation Sequencing Labs
Patented SPRI chemistry is combined with the flexibility
of the Beckman Coulter Biomek FXP liquid handler to
revolutionize how next generation sequencing samples
are prepared. All chemistry needed to prepare libraries is
included in a kit format along with the reagents to perform
size selection and chemistry cleanup. The only thing left to
do is add the DNA sample and adapter of choice.
Because SPRIworks HT is automated on the Biomek
automated liquid handler, users have access to a suite
of methods to further streamline the next generation
sequencing workflow. Such methods include: PCR setup,
PCR (on deck with T-robot integration), PCR cleanup,
quantitation, normalization, and sample pooling.
Fully automated library construction process
With an Intuitive Easy to Use
Interface, Everyone is an Expert
• Less than 30 minutes of hands-on time
High throughput
Step 1. Configure parameters using the intuitive user interface
• Up to 96 high quality, fully constructed and sized
libraries in under 6 hours
• Up to 25 times the throughput of manual library
construction, similar to having 25 additional people
to do next generation sequencing sample preparation
Simple graphical user interface
150-350 size selection
Short fragment sizes isolated using SPRIworks HT High Throughput
System and analyzed using the Agilent High Sensitivity DNA Chip.
Illumina paired end adapters were used, samples were purified
with Agencourt AMPure XP and amplified with 10 cycles of PCR.
Includes SPRI size selection and cleanup
High Throughput Library Construction
Up to 96 high quality, fully constructed and sized libraries
in under 6 hours.
SPRIworks HT
Throughput
Up to 96 samples in under 6 hours
DNA input amount
1 µg
Size selection options
• No size selection
• 150-350 bp (insert sizes)
• 250-450 bp (insert sizes)
• 350-700 bp (insert sizes)
Supported input sample types
Sheared DNA, gDNA, cDNA, amplicons
Step 2. Method calculates reagent volumes based on user input
Step 3. Place tips, plates and reagents on the instrument, click OK
Ordering Information
In the United States and Canada call +1 800 361 7780
In other countries, please contact your local representative or call the country number listed below.
SPRIworks Fragment Library Systems (Up to 30 libraries/day)
Product #
SPRIworks System I (Includes instrument, cartridges, method card and labware)
SPRIworks Fragment Library kit I (For Illumina Genome Analyzer) 10 cartridges
SPRIworks Fragment Library kit I (For Illumina Genome Analyzer) 50 cartridges
A88267
A84801
A84803
SPRIworks System II (Includes instrument, cartridges, method card and labware)
SPRIworks Fragment Library kit II (For Roche GS FLX DNA Sequencer) 10 cartridges
SPRIworks Fragment Library kit II (For Roche GS FLX DNA Sequencer) 50 cartridges
A88268
A84805
A84806
SPRIworks System III (Includes instrument, cartridges, method card and labware)
SPRIworks Fragment Library kit III (For Life Technologies SOLiD Sequencer) 10 cartridges
SPRIworks Fragment Library kit III (For Life Technologies SOLiD Sequencer) 50 cartridges
A98473
A98476
A98477
SPRIworks HT System (Up to 96 libraries/day)
Product #
SPRIworks HT Reagent kit (For Illumina next generation sequencers) 48 samples
B06938
Biomek FXP Dual Arm Multi-96 and Span-8 Workstation - SPRIworks HT1
B054502
SPRIworks HT Automation Consumables (Includes Biomek tips, plates, tubes, etc. to process 960 samples)
B05451
1
2
Contact your automation sales specialist for specific configurations. +1 800 742 2345
The Biomek Workstation (Product # B05450) is intended for 110V operation only.
For more information, please visit www.beckmancoulter.com or contact your local sales representative.
For technical support in the United States and Canada call +1 800 854 3633 (Option #, 1, 3, 3).
In other countries, please contact your local representative or call the country number listed below.
† The PCR process is covered by patents owned by Roche Molecular Systems, Inc., and F. Hoffman-La Roche, Ltd.
* All trademarks are property of their respective owners.
Beckman Coulter, the stylized logo, AMPure, Biomek, SPRI and SPRIworks are registered trademarks of Beckman Coulter, Inc.
For Research Use Only. Not for use in diagnostic procedures.
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