Custom BioGenic Systems manufactures a wide range of products

Slide 4 of 89
Custom BioGenic Systems manufactures a
wide range of products for industries that
include; life sciences, research laboratories,
veterinarian, horticulture, pharmaceutical,
industrial, scientific and medical, as well as cell
and tissue banks.
 CBS Isothermal -196°C dry storage freezers
 CBS Standard liquid nitrogen freezers
 Cryosystems, vapor-shippers and lab tanks
 Controlled rate freezers
 Electronic controls, alarms and data recorders
 Freezer racks and inventory systems
CBS Isothermal
Dry Storage Freezers
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Why is dry storage important?
Safety. Storage in liquid nitrogen can make vials shrink. This may
cause liquid nitrogen to seep into the vials, which on rewarming,
expand and explode as nitrogen vaporizes inside the vials. Dry
storage eliminates this possibility. Also, the Isothermal design
provides added user safety by eliminating contact with or
splashing of liquid nitrogen.
Cross Contamination. Studies have shown that viral, bacterial
and fungal pathogens can survive after suspension in liquid
nitrogen. Infected samples can cross contaminate other samples
in the same liquid nitrogen tank. Dry storage eliminates the
possibility of cross contamination.
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CBS Isothermal -196C Dry Storage Freezers
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Custom BioGenic Systems -196C Isothermal
Dry Storage Freezers Specifications
V1500-AB
V3000-AB
30
70
89
93
140
External Width in (mm) 26 (660)
37 (939)
37 (939)
47 (1,219)
47 (1,219)
External Depth in (mm) 37 (939)
48 (1,219)
48 (1,219)
54 (1,371)
54 (1,371)
External Height in (mm) 45 (1,143)
47.5
(1,206)
58 (1,473)
52 (1,320)
58 (1,473)
Usable Interior Height in (mm) 29 (736)
29 (736)
37 (940)
29 (736)
34 (864)
Usable Interior Diameter in (mm) 21 (534)
31 (787)
31 (787)
40 (1,016)
40 (1,016)
Weight Empty lbs(kg) 327 (148)
600 (272)
650 (295)
936 (425)
997 (453)
Weight Full lbs(kg) 383 (174)
720 (327)
808 (367)
1100 (500)
1247 (566)
9,100
22,100
25,500
40,300
46,500
434
1,120
1,280
1,936
2,208
LN2 Capacity (liters)
Maximum 2ml vial capacity
Maximum 50ml bag capacity
V3000EH-AB V5000-AB V5000EH-AB
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Know the CBS Isothermal Freezer
The unique, patented design provides the optimum environment for
preserving the long-term viability of samples with uniform temperatures
throughout the freezer in the -190°C range, including directly below the lid.
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Know the CBS Isothermal Freezer
No liquid nitrogen inside the
sample area eliminates the risk
of cross contamination and
provides added user safety by
eliminating contact with or
splashing of liquid nitrogen.
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Know the CBS Isothermal Freezer
The full internal volume is
available for storing samples
making the most efficient use
of freezer capacity. There are
no aluminum plates or
carousel walls taking up
valuable storage space.
The full width lid provides
unrestricted access to
samples.
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Know the CBS Isothermal Freezer
The outstanding
temperature uniformity and
“dry” storage condition
address requirements of
the latest European
directives for tissue
storage, in respect to
maintaining cell integrity
and preventing
contamination.
Slide 15 of 89
Temperature Uniformity and Liquid Level
In conventional (non CBS) vapor phase freezers, the
temperature directly under the lid can be reduced by raising the
level of liquid Nitrogen in the bottom.
Whilst improving the temperature uniformity (gradient), this
reduces the amount of space available for storing samples in
vapor. This also makes the freezer more expensive in terms of
the number of samples stored.
In this situation, ask the customer to find out the relationship
between temperature gradient and the level of liquid Nitrogen
in the freezer, since the specified temperature may depend
heavily on the liquid Nitrogen level.
In CBS Isothermal freezers all the internal volume is available
for sample storage - free from liquid Nitrogen.
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Temperature Uniformity and Temperature Probe Location
Encourage the customer to check with potential suppliers
about the operating temperatures of their freezers and where
the temperature sensor is located.
Cold air sinks and therefore the best location for the
temperature sensor is at the top, above the samples. On the
CBS Isothermal freezers, the sensor is located directly below
the lid and above the top of the racks giving the customer
confidence that all samples are stored below the indicated
{display) temperature - see typical V-1500 mapping data
below.
If the sensor is positioned lower down in the freezer, the
displayed temperature will look good but can the customer
be sure of the temperature of samples higher up?
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CBS Isothermal -190C Dry Storage Freezers
Fill to Fill Temperature Study
Probe #1
Probe #2
Probe #3
Probe #4
Probe #5
Probe #6
An aluminum freezer rack is used for
attaching 6 temperature probes to monitor
liquid nitrogen vapor temperature.
Spacing between the probes is
approximately 4 inches (10 cm).
Slide 18 of 89
CBS Isothermal -190C Dry Storage Freezers
Fill to Fill Temperature Study
-178°C
-180°C
START OF FILL
LEVEL AT 10"(25CM)
END OF FILL
LEVEL AT 17"(43CM)
FILLING PROGRAM - LOW SET POINT 10" (25 CM)
HIGH SET POINT 17" (43 CM)
-182°C
Probe 1
-184°C
Probe 2
-186°C
Probe 3
-188°C
Probe 4
Probe 5
-190°C
Probe 6
-192°C
-194°C
-196°C
10 Minute
fill period
Approximately
21 to 24 hours
fill to re-fill
6 Temperature probes are placed in a 13 shelf aluminum
freezer rack, equally spaced from top to bottom.
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Conclusion
This study shows that as the liquid nitrogen in your CBS Isothermal
Freezer evaporates, the internal temperature at probe #1 will rise
slightly to -182C. As the re-fill process starts, the temperature at
probe #1 will return to the normal operating temperature in the -189C
range.
The temperature of the other 5 probes increases very little, ranging
between -186C to -196C. Therefore, 85% of the freezer storage
space remains below -186C and only the top15% of the space will be
slightly warmer in the -182C range.
This study provides temperature data that confirms samples stored in
a CBS Isothermal Freezer will be well below critical temperatures.
Your CBS Isothermal Freezer is pre set to re-fill when the liquid
nitrogen level reaches a low set point of 10” (25cm) and stop filling
when the liquid nitrogen reaches the high set point of 17” (43cm).
These filling set points will provide excellent operating parameters.
However, they can be changed to suit your specific requirements.
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Temperature Uniformity and Thermal Conductivity
Customer: "Other freezers also offer -190°C vapor storage"
To compete with the Isothermal concept and provide greater
uniformity, other manufacturers use the thermal conductivity properties
of aluminum and install aluminum plates (frames and carousel walls)
inside the freezer. The aluminum conducts heat from the top of the
freezer, which promotes evaporation and the resulting Nitrogen vapor
rises to cool the top of the freezer.
This approach has two disadvantages;
• Firstly, the more aluminum in contact with liquid Nitrogen, the
higher the consumption will be. This is why it is important for the
customer to compare the loss rate of the actual models they are
considering.
• Secondly, the use of frames and carousels reduces the amount of
space inside the freezer for samples. In contrast, the open interior
of the CBS Isothermal freezer provides maximum storage capacity
without the restrictions of carousels etc.
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Narrow Neck Lids
Customer: " I prefer Narrow Neck Lids as they keep the cold in"
In an effort to reduce cold air loss and liquid Nitrogen consumption,
manufacturers have introduced models with narrow neck openings. Whilst the
concept appears attractive, feedback from many customers highlights some
practical issues with this design.
Firstly, access to and location of samples is made more difficult. In contrast,
the CBS Isothermal design with Nitrogen vapor vents helps to hold the cold air
inside the freezer. This means that full-width lids can be used giving the user
unrestricted access for easier sample location.
The following temperature mapping data shows how the temperature inside a
V-1500 increases on lid opening. Even after 30 minutes the air temperature at
the top of the V-1500 remains well below the critical glass transition
temperature of water.
Secondly, if anything is dropped or becomes jammed in the carousel system of
a freezer with a narrow neck lid, it becomes almost impossible to retrieve or
repair.
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CBS Isothermal -190C Dry Storage Freezers
5 Minute Lid Open Temperature Study
Probe #1
Probe #2
Probe #3
Probe #4
Probe #5
Probe #6
An aluminum freezer rack is used for
attaching 6 temperature probes to monitor
liquid nitrogen vapor temperature.
Spacing between the probes is
approximately 4 inches (10 cm).
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CBS Isothermal -190C Dry Storage Freezers
5 Minute Lid Open Temperature Study
17:12:00
17:06:00
17:00:00
16:30:00
16:00:00
15:30:00
15:00:00
14:30:00
14:00:00
13:30:00
13:00:00
12:30:00
12:00:00
-180°C
0:30:00
-178°C
-182°C
-184°C
TEMPERATURE
INCREASES
COLD VAPOR FROM THE
LOWER LAYERS COOLS
THE UPPER LAYERS
TEMPERATURE IS
STABILIZED TO
NORMAL OPERATING
TEMPERATURE
-186°C
-188°C
Probe 1
Probe 2
Probe 3
Probe 4
Probe 5
-190°C
Probe 6
-192°C
-194°C
-196°C
5 MINUTE LID OPEN PERIOD
Freezer lid is opened.
Items are stored or retrieved.
Lid is closed.
6 Temperature probes are placed in a 13
shelf aluminum freezer rack, equally
spaced from top to bottom.
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Narrow Neck Lids
 Lower capacity
 Round lid opening may restrict access to square
racks
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Liquid Nitrogen Consumption
Customer: “Other freezers use less liquid nitrogen”
The only way to measure and compare LN2 consumption of freezers is by
using the Static Evaporation Rate (also known as Normal Evaporation Rate).
This is an industry accepted method for measuring the LN2 consumption of an
empty freezer under standardized conditions. (A copy of the method is
available on request).
Many manufacturers make claims about lower LN2 consumption and running
costs but then don't support this with data in their sales material.
If LN2 consumption is an issue, we recommend that customers ask potential
suppliers for the Static Evaporation Rate (SER) for the freezers they are
considering so that like-for like comparisons can be made.
It is important that the customer asks for the SER of the specific model they are
considering, including built-in options. Some manufacturers only quote a SER
for the base model, however the actual SER could be much higher if the
customer orders optional aluminum frames or a carousel.
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Series 2301 Auto-Fill and Monitor System
2301 Front Panel Display
Four line LCD readout
 Start / Stop Fill
 System Status
 Output Data to Printer
 Fill Level Set Points
 Temperature Display – Fahrenheit / Centigrade
 Liquid Level – Inch / Centimeter
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Liquid Level Filling Set Points
Your CBS Isothermal Freezer is delivered with factory pre-set liquid
level filling set points.
 The low set point (start fill) should be set at 10 inches (25 cm).
 The high set point (stop fill) should be set at 17 inches (43 cm).
These filling set points will provide excellent operating parameters.
However, they can be changed to suit your specific requirements.
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Series 2301 Controller
2301 Features
New 16 Port Connector
 0-5 volt outputs provide temperature & level data for connection to existing alarm/monitor system
 4-20 mA outputs provide temperature & level data for connection to existing alarm/monitor system
 Sequential and One-Fill-All-Fill input and output connection for filling systems
New two level temperature display option
 Temp A displays temperature at the lid, approximately 11” (27.94 cm)
 Temp B displays temperature inside the storage space, approximately 20” (50.80 cm)
Additional Features
 Download data files to PC and be able to convert them to .csv files for use in EXCEL
 Ethernet and auxiliary RS485 for future expansion
 Dedicated 24VDC signal output to activate a third 24VDC valve to control the LN2 supply
 Global Remote Alarm - Dry contact that switches during any alarm condition
 Overflow Sensor (Optional)
 Fill Timer
 Data Log - Report Temp A, Temp. B and level with date and time stamp. Printed or displayed.
Slide 29 of 89
Series 2301 Features / Specifications
1
18
1.
2.
3.
4.
5.
6.
7.
8.
9.
10.
11.
2
19
3
4
5
17
6
7
9
10
8
Vent Thermocouple
Temp. A Thermocouple - Lid temp (located 11” down)
Temp. B Thermocouple - Temp (located 20” down)
Ethernet Port - For future expansion
RS-485 IN
RS-485 OUT
Sensor Port
Lid Switch
Fill/Vent In Progress
Fill Solenoid Valve Outlet
Vent Solenoid Valve Outlet
11
16
12.
13.
14.
15.
16.
17.



18.
19.
12
15
13
14
Global Remote Alarm
2 Amp Fuse
24 V Power Supply
Overflow Sensor (Optional)
Thermal Printer Port - For printing alarms & data
16 Port Connector:
0-5V output for Temp. A, Temp. B, and Level
4-20mA outputs – for Temp. A, Temp. B, and Level
Seq. OFAF Input and Output Connection
Auxiliary RS 485 - For future expansion
USB Port
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Initial Filling Details
The amount of time required to fill a freezer with liquid
nitrogen will vary with model. Listed below are the
estimated fill times for each CBS Isothermal Freezer.
 V1500 – approximately 40 minutes
 V3000 – approximately 90 minutes
 V5000 – approximately 90 minutes
 V5000 EH – approximately 90 minutes
These fill times are based on a conventional 180 liter
and 230 liter supply tanks operating at 22 psi.
Fill times may vary with the use of bulk tanks and
vacuum jacketed supply lines.