Headstage Technical Guide

Transcription

8, 16, 32 and 96 Channel Headstages
www.plexon.com
Contents
4Introduction
5
Headstage A – 8 channel, 0.050” pitch
7
8
R00 Permutation – Ground Reference Only
9
R01 Permutation – Ground Reference and True Reference
10
R10 Permutation – True Reference and Ground Reference
11
R11 Permutation – True Reference Only
12
Headstage B – 8 channel, 0.050” pitch (Latching)
13
Headstage C – 8 channel, 0.025” pitch (9 data pins)
15
Headstage C2 – 8 channel, 0.025” pitch (9 data pins)
17
Headstage D – 8 channel, 0.025” pitch (10 data pins)
19
Headstage D2 – 8 channel, 0.025” pitch (10 data pins)
21
Headstage E – 16 channel, 0.025” pitch
24
Headstage E2a – 16 channel, 0.025” pitch (2 Guide Pins)
26
Headstage E2b – 16 channel, 0.025” pitch (6 Guide Pins)
28
Headstage F – 32 channel, 0.025” pitch
31
Headstage F3 – 32 channel, 0.025” pitch
34
Headstage H – 16 channel, 0.025” pitch (VLSI)
36
Headstages J – 32 channel, 0.025” pitch (VLSI)
39
Headstage M – 96 channel, 0.050” pitch
42
Headstage N - 8 channel, 0.049” pitch (Biopotential) - PNS
44
Headstage P - 8 channel, 0.049” pitch (Biopotential) - EDIF
46
Headstage Q – 16 channel, 0.025” pitch (Digital)
48
Headstage R – 32 channel, 0.025” pitch (Digital)
50
PRA Headstage Preamplifier Board
51
PRA2 Headstage Preamplifier Board
53
55
Biopotential Headstage Electrode Interface Board (EIB) - Single Input
56
Biopotential Headstage Electrode Interface Board (EIB) - Dual Input
57
Headstage and Conversion Options
57
Light Emitting Diode (LED)
58
Latching
58
Ground Reference to True Reference Conversion
59DigiAmp™ Connector Pin-out, Ground and Reference Information
61MiniDigi™ Connector Pin-out, Ground and Reference Information
www.plexon.com
Page 2
Documentation History
Date
Version
Notes
Author
April 2015
HSTTN0001h
- Minor edits to headstages, cables and pin-out graphics.
Yolanda Rowe
February 2015
HSTTN0001g
- Added Digiamp™ and MiniDigi™ Connector Pin-out, Ground and
Reference Information pages.
- Added Headstages C2 and D2
- Added output pin-out to digital Headstages Q and R.
Yolanda Rowe
October 2014
HSTTN001f
- Renamed Headstages N and P to Q and R.
- Added new biopotential headstages.
- Added Electrode Interface Boards section.
- Minor edits to Dimensions for Headstages A, B, E2a, E2b, H and M
Yolanda Rowe
August 2014
HSTTN0001e
- Added 16 and 32 channel digital Headstages N and P.
- Added Headstage Type and Amplifier/Processor Compatibility
to Headstages A through P.
Yolanda Rowe
October 2013
HSTTN0001d
- Minor edits to Headstages M and headstage preamplifier sections.
Yolanda Rowe
August 2013
HSTTN0001c
- Added input impedances to Headstages E, E2a, E2b, F, F3 and M.
- Minor edits to Headstages H, J and M.
Yolanda Rowe
June 2013
HSTTN0001b
- Content edits and added Headstages E2a, E2b, F3 and M.
Yolanda Rowe
March 2013
HSTTN0001a
- New content changes with minor updates.
- Reformatted document to current branding guidelines.
Yolanda Rowe
June 2009
v1.0
- Initial creation of document for 8, 16 and 32 channel headstages and
PRA Boards, Connector Pin-out, Ground and Reference Information.
Chris Heydrick
www.plexon.com
Page 3
Introduction
Plexon’s analog and digital headstages serve to amplify neural and reference signals to optimize the recording of
the signal for successful travel along the length of a headstage cable with minimal interference. The various models
of headstages have different options for references. The following Headstage Technical Guide will serve to describe
those options and delineate the settings that are applied to the post-Headstage front end hardware Plexon provides
in order to use a particular reference.
The Headstage Technical Guide references the various Headstages, Connectors and cables as featured on our website
at www.plexon.com. Not detailed on our website (but delineated in this guide) are the various permutations available
for the 16 channel, 0.050” pitch headstage. The 16 channel, 0.050” pitch headstage is actually two 8 channel,
0.050” pitch headstages (Headstage A) permanently stacked. Permutations (Rxx) are the direct result of stacking
two 8 channel headstages, resulting in any one of the following four combinations below where 0 represents ground
reference and 1 represents true reference:
R00 = Ground Reference only
R01 = Ground Reference and True Reference
R10 = True Reference and Ground Reference
R11 = True Reference only
Connector pin-out configurations, including ground and reference information for each headstage are pictured and
outlined along with a section describing the connections and pinouts on the front panel of each of the DigiAmp™
and MiniDigi™ Digitizing Amplifers. Three headstage preamplifier boards (PRA, PRA2 and PRA3) featuring various
noseboards and jumper/referencing information are also featured as well as the most commonly used Electrode
Interface Boards which facilitate connection of electrodes to Plexon’s biopotential/differential Headstages N and P.
Lastly, the Headstage Technical Guide highlights several options to our headstages that include:
Mounted LEDs for most of our analog and digital headstages for use in behavioral analysis research using
Plexon’s CinePlex® Behavioral Research System with CinePlex Tracking. Your choice of red, blue or green LEDs
is available;
t he ever popular clip (latching mechanism) to ensure added security with more active animals (available for
both the low-impedance and high-impedance, 0.050” pitch headstages); and
converting a Ground Reference to True Reference.
NOTE: The product images in this document are not to scale relative to either actual size or to each other. The
headstage lengths include the longest pin. The headstage weights may vary ever so slightly due to the hand
crafted nature of the products. The addition of LEDs and/or a latch may impact the weight or increase the
dimensions of the headstage slightly, and references to preamplifers are MAP specific.
www.plexon.com
Page 4
Gain 1x (G1)
Gain 20x (G20) –
lavender dot in center
Channels:
8
Headstage Type: Analog
Pitch:
0.050”
Data pins:
10
Guide pins:
2
Amplifier/Processor Compatibility:
DigiAmp™, MiniDigi™,
Analog Amplifier,
PBX, PBX2, PBX3
Catalog Name Format:
HST/8o50-Gx-xR
RMS Noise (with inputs grounded):
2.5μV rms for 0.1Hz to 10kHz
Gain:
1x or 20x
Reference:
Ground or True
Power Supply:
±1.5V to ±7.5V
Supply Current:
3mA
Input Impedance (G1):
45MΩ @ 1kHz
Output Impedance (G1):
240Ω @ 1kHz
24MΩ @ 1kHz
50Ω @ 1kHz
Dimensions:
0.96L x 0.64W x 0.075D inches
(24.4L x 16.3W x 1.9Dmm)
Weight:
0.036oz
(1.02g)
Description:
An 8 channel op-amp based headstage amplifier containing eight input pins tied to a gain of 1x or 20x amplifier
designed to record from high impedance electrodes.
Input Connector Information: Connector #2
Input Mating Connector:
Male
Catalog Name:
CON/8o50m-10P
Input Connector:
Female
Catalog Name:
CON/8o50f-10P
Headstage Cable Information:
Catalog Name:
HSC/8
www.plexon.com
Page 5
Connector Pin-out, Ground and Reference Information
Ground Reference (GR)
Two pins are connected to a ground line whose output is a unity gain buffered ground that is used differentially at the
preamplifier level.
HST/8o50-G1-GR
50982
20x Gain Catalog name:
Item #:
HST/8o50-G20-GR
50988
Guide Pin Hole
1 – Channel 1 Out
2 – Channel 2 Out
3 – Channel 3 Out
4 – Channel 4 Out
5 – Channel 5 Out
6 – Channel 6 Out
7 – Channel 7 Out
9 – Buffered Ground
10 – (+5V)
11 – (-5V)
12 – Ground
Guide Pin Hole
Guide Pin
10 – Ground
9 – Ground
OUTPUT
8 – Channel 8 In
7 – Channel 7 In
6 – Channel 6 In
5 – Channel 5 In
4 – Channel 4 In
3 – Channel 3 In
2 – Channel 2 In
1 – Channel 1 In
Guide Pin
INPUT
8 – Channel 8 Out
Item:
Input Pin 9: Ground (output pin 9)
his pin is internally tied to system ground input pin 10 and is unity gain (1x) amplified and output from the headstage pin 9 on the
T
output connector. We call this output the “Buffered Ground”.
Use this pin to ground the animal via skull screw or cannula, and make sure it touches the cerebrospinal fluid.
et the preamplifier reference jumper to “REF1” to use this as a reference if the headstage is plugged into the channel 1-8 input of
S
the preamplifier, or “REF2” if it is plugged into the channel 9-16 input of the preamplifier.
Input Pin 10: Ground
This pin is internally tied to input pin 9, and tied to the system ground.
True Reference (TR)
One pin is tied to a unity gain (1x) amplifier for a reference electrode that is used differentially at the preamplifier level,
and another pin is tied to ground.
HST/8o50-G1-TR
50986
Item #:
HST/8o50-G20-TR
50992
Guide Pin Hole
1 – Channel 1 Out
2 – Channel 2 Out
3 – Channel 3 Out
4 – Channel 4 Out
5 – Channel 5 Out
6 – Channel 6 Out
7 – Channel 7 Out
9 – Reference
10 – (+5V)
11 – (-5V)
12 – Ground
Guide Pin Hole
Guide Pin
10 – Ground
9 – Reference
8 – Channel 8 In
OUTPUT
7 – Channel 7 In
6 – Channel 6 In
5 – Channel 5 In
4 – Channel 4 In
3 – Channel 3 In
2 – Channel 2 In
1 – Channel 1 In
Guide Pin
INPUT
8 – Channel 8 Out
Item #:
Input Pin 9: Reference (output pin 9)
This is typically connected to a de-insulated electrode and used as a local reference for spike channels.
This Reference is unity gain (1x) amplified and output from the headstage (pin 9 on the output connector).
et the preamplifier reference jumper to “REF1” to use this as a reference if the headstage is plugged into the channel 1-8 input of
S
the preamplifier, or “REF2” if it is plugged into the channel 9-16 input of the preamplifier.
Use this pin to ground the animal via skull screw or cannula, and make sure it touches the cerbrospinal fluid.
This pin is tied to the system ground.
www.plexon.com
Page 6
Gain 1x (G1)
Gain 20x (G20) –
Channels:
16
Pitch:
0.050”
Data pins:
20
Guide pins:
4
Analog Amplifier,
PBX, PBX2, PBX3
HST/16o50-Gx-Rxx
Gain:
1x or 20x
Reference:
R00, R01, R10, R11
Power Supply:
±1.5V to ±7.5V
Supply Current:
6mA
45MΩ @ 1kHz
240Ω @ 1kHz
24MΩ @ 1kHz
50Ω @ 1kHz
Dimensions:
0.96L x 0.64W x 0.075D inches
(24.4L x 16.3W x 1.9Dmm)
Weight:
0.09oz
(2.55g)
Description:
A 16 channel op-amp based headstage amplifier containing 16 input pins tied to a gain of 1x or 20x amplifier
designed to record from high impedance electrodes. This same 16 channel headstage can be configured into various
ground and/or true reference permutations (Rxx): R00, R01, R10 and R11. Permutations are the direct result of
stacking two 8 channel headstages, resulting in any one of the following four combinations below where 0 represents
ground reference and 1 represents true reference:
R00 = Ground Reference only
R01 = Ground Reference and True Reference
R10 = True Reference and Ground Reference
R11 = True Reference only
Connector is available in stacked 16 and 32 channel configurations using CON/8o50m-10P and/or CON/8o50f-10P.
Male
Catalog Name:
CON/8o50m-10P
Input Connector:
Female
Catalog Name:
CON/8o50f-10P
Catalog Name:
HSC/16o50
www.plexon.com
Page 7
R00 Permutation - Ground Reference (GR) Only
Four pins are connected to a ground line whose output is a unity gain (1x) buffered ground that is used differentially
at the preamplifier level.
HST/16o50-G1-R00
50948
Item #:
HST/16o50-G20-R00
50952
1 – Channel 1 Out
2 – Channel 2 Out
3 – Channel 3 Out
4 – Channel 4 Out
5 – Channel 5 Out
6 – Channel 6 Out
7 – Channel 7 Out
8 – Channel 8 Out
Guide Pin Hole
Guide Pin Hole
Channel 9 Out – 13
10 – (+5V)
Buffered Ground – 21
11 – (-5V)
(+5V) – 22
12 – Ground
Guide Pin Hole
(-5V) – 23
Ground – 24
8 – Channel 8 In
Guide Pin Hole
7 – Channel 7 In
Channel 16 In – 18
Guide Pin
6 – Channel 6 In
10 – Ground
5 – Channel 5 In
Guide Pin
4 – Channel 4 In
9 – Ground
3 – Channel 3 In
Ground – 20
2 – Channel 2 In
Ground – 19
1 – Channel 1 In
Guide Pin
Guide Pin
OUTPUT
Channel 9 In – 11
INPUT
Item #:
his pin is internally tied to system ground (input pin 10) and is unity gain (1x) amplified and output from the headstage
T
(pin 9 on the output connector). We call this output the Buffered Ground.
Set the preamplifier reference jumper to “REF1” to use this as a reference.
T
www.plexon.com
Page 8
R01 Permutation - Ground Reference and True Reference
One pin tied to a unity gain (1x) amplifier for a reference electrode that is used differentially at the preamplifier level,
1 pin connected to a ground line that is output as a unity gain ground that is used differentially at the preamplifier
level, and 2 pins tied to ground.
HST/16o50-G1-R01
TBD
Item #:
HST/16o50-G20-R01
TBD
1 – Channel 1 Out
2 – Channel 2 Out
3 – Channel 3 Out
4 – Channel 4 Out
5 – Channel 5 Out
6 – Channel 6 Out
7 – Channel 7 Out
8 – Channel 8 Out
Guide Pin Hole
Guide Pin Hole
10 – (+5V)
Reference – 21
11 – (-5V)
(+5V) – 22
12 – Ground
Guide Pin Hole
(-5V) – 23
Ground – 24
8 – Channel 8 In
Guide Pin Hole
7 – Channel 7 In
Guide Pin
6 – Channel 6 In
10 – Ground
5 – Channel 5 In
Guide Pin
4 – Channel 4 In
9 – Ground
3 – Channel 3 In
Ground – 20
2 – Channel 2 In
Reference – 19
1 – Channel 1 In
Guide Pin
Guide Pin
OUTPUT
Channel 9 In – 11
INPUT
Item #:
T
www.plexon.com
Page 9
R10 Permutation - True Reference and Ground Reference
One pin tied to a unity gain (1x) amplifier for a reference electrode that is used differentially at the preamplifier level,
1 pin connected to a ground line that is output as a unity gain (1x) ground that is used differentially at the preamplifier
level, and 2 pins tied to ground.
HST/16o50-G1-R10
50950
Item #:
HST/16o50-G20-R10
50952-001
6 – Channel 6 Out
5 – Channel 5 Out
4 – Channel 4 Out
3 – Channel 3 Out
2 – Channel 2 Out
1 – Channel 1 Out
Guide Pin Hole
7 – Channel 7 Out
Guide Pin Hole
8 – Channel 8 Out
9 – Reference
10 – (+5V)
11 – (-5V)
(+5V) – 22
12 – Ground
Guide Pin Hole
(-5V) – 23
Ground – 24
8 – Channel 8 In
Guide Pin Hole
7 – Channel 7 In
Guide Pin
6 – Channel 6 In
10 – Ground
5 – Channel 5 In
Guide Pin
4 – Channel 4 In
9 – Reference
3 – Channel 3 In
Ground – 20
2 – Channel 2 In
Ground – 19
1 – Channel 1 In
Guide Pin
Guide Pin
OUTPUT
Channel 9 In – 11
INPUT
Item #:
Set the preamplifier/reference jumper to “REF1” to use this as a reference.
T
www.plexon.com
Page 10
R11 Permutation - True Reference (TR) Only
Two pins tied to unity gain (1x) amplifier for a reference electrode that is used differentially at the preamplifier level,
and 2 pins tied to ground.
HST/16o50-G1-R11
50951
Item #:
HST/16o50-G20-R11
99134
6 – Channel 6 Out
5 – Channel 5 Out
4 – Channel 4 Out
3 – Channel 3 Out
2 – Channel 2 Out
1 – Channel 1 Out
Guide Pin Hole
7 – Channel 7 Out
Guide Pin Hole
8 – Channel 8 Out
9 – Reference #1
10 – (+5V)
Reference #2 – 21
11 – (-5V)
(+5V) – 22
12 – Ground
9 – Reference #1
(-5V) – 23
8 – Channel 8 In
Reference #2 – 19
Guide Pin Hole
7 – Channel 7 In
Ground – 24
6 – Channel 6 In
Guide Pin Hole
5 – Channel 5 In
Guide Pin
4 – Channel 4 In
10 – Ground
3 – Channel 3 In
Guide Pin
2 – Channel 2 In
Ground – 20
1 – Channel 1 In
Guide Pin
Guide Pin
OUTPUT
Channel 9 In – 11
INPUT
Item #:
Input Pin 9: Reference #1 (output pin 9)
Set the preamplifier/reference jumper to “REF1” to use this as a reference.
www.plexon.com
Page 11
Headstage B – 8 channel, 0.050” pitch, Latching
Gain 1x (G1)
Headstage A with latch
Gain 20x (G20) –
Headstage A with latch
Channels:
8
Pitch:
0.050”
Data pins:
10
Guide pins:
2
Analog Amplifier,
PBX, PBX2, PBX3
HST/8o50-Gx-xR-Lxx
Gain:
1x or 20x
Reference:
Ground or True
Power Supply:
±1.5V to ±7.5V
Supply Current:
3mA
45MΩ @ 1kHz
240Ω @ 1kHz
24MΩ @ 1kHz
50Ω @ 1kHz
Dimensions:
0.96L x 0.64W x 0.075D inches
(24.4L x 16.3W x 1.9Dmm)
Weight:
0.036oz
(1.02g)
Description:
An 8 channel op-amp based headstage amplifier containing eight input pins tied to a unity gain (1x) amplifier
designed to record from high impedance electrodes. Plexon’s 0.050” pitch, 8 channel headstages are identical to
Headstage A but comes with a special clip (latch) mechanism for added security with more active animals.
Male
Catalog Name:
CON/8o50m-10P-L
Input Connector:
Female
Catalog Name:
CON/8o50f-10P-L
Headstage Cable Information: Reference Headstage A
As Headstage B only differs from Headstage A due to the addition of a latch option, please refer to Headstage A on
page 4 of this document for this information.
Connector Pin-out, Ground and Reference Information: Reference Headstage A
As Headstage B only differs from Headstage A due to the addition of a latch option, please refer to Headstage A on
page 4 of this document for this information.
www.plexon.com
Page 12
Headstage C – 8 channel, 0.025” pitch, 9 data pins
Gain 1x (G1)
Channels:
8
Pitch:
0.025”
Data pins:
9
Guide pins:
2
Analog Amplifier,
PBX, PBX2, PBX3
HST/8o25-9P-xR
Gain:
1x
Reference:
Ground or True
Power Supply:
±1.5V to ±7.5V
Supply Current:
3mA
Input Impedance:
40MΩ @ 1kHz
Output Impedance:
240Ω @ 1kHz
Bandwidth:
0Hz – 1MHz
Dimensions:
0.76L x 0.46W x 0.07D inches
(19.3L x 11.7W x 1.8D mm)
Weight:
0.017oz
(0.47g)
Description:
Male
Catalog Name:
CON/8o25m-9P
Input Connector:
Female
Catalog Name:
CON/8o25f-9P
Catalog Name:
HSC/8
www.plexon.com
Page 13
A single unity gain (1x) buffered ground pin that is used differentially at the preamplifier level.
Item #:
HST/8o25-9P-GR
99036
Guide Pin Hole
1 – Channel 1 Out
2 – Channel 2 Out
3 – Channel 3 Out
4 – Channel 4 Out
5 – Channel 5 Out
6 – Channel 6 Out
7 – Channel 7 Out
8 – Channel 8 Out
9 – Ground
10 – (+5V)
11 – (-5V)
12 – Ground
Guide Pin Hole
Guide Pin
9 – Ground
8 – Channel 8 In
OUTPUT
7 – Channel 7 In
6 – Channel 6 In
5 – Channel 5 In
4 – Channel 4 In
3 – Channel 3 In
2 – Channel 2 In
1 – Channel 1 In
Guide Pin
INPUT
This Ground is unity gain (1x) amplified and output from the headstage (pin 9 on the output connector).
This is typically used as a distant reference for field potentials, and also for spike channels.
et the preamplifier reference jumper to “REF1” to use this as a reference if the headstage is plugged into the channel 1-8
S
input of the preamplifier, or “REF2” if it is plugged into the channel 9-16 input of the preamplifier.
True Reference (TR)
A single pin is tied to a unity gain (1x) input for a reference electrode pin that is used differentially at the preamplifier
level.
Item #:
HST/8o25-9P-TR
50979
Guide Pin Hole
1 – Channel 1 Out
2 – Channel 2 Out
3 – Channel 3 Out
4 – Channel 4 Out
5 – Channel 5 Out
6 – Channel 6 Out
7 – Channel 7 Out
8 – Channel 8 Out
9 – Reference
10 – (+5V)
11 – (-5V)
12 – Ground
Guide Pin Hole
Guide Pin
9 – Reference
8 – Channel 8 In
OUTPUT
7 – Channel 7 In
6 – Channel 6 In
5 – Channel 5 In
4 – Channel 4 In
3 – Channel 3 In
2 – Channel 2 In
1 – Channel 1 In
Guide Pin
INPUT
S
input of the preamplifier, “REF2” if it is plugged into the channel 9-16 input of the preamplifier.
CAUTION: This input connector provides no ground connection to the animal.
www.plexon.com
Page 14
Headstage C2 – 8 channel, 0.025” pitch, 9 data pins
Channels:
8
Pitch:
0.025”
Data pins:
9
Guide pins:
2
Analog Amplifier,
PBX, PBX2, PBX3
HST/8o25-GEN29P-G1-xR
Gain:
1x
Reference:
Ground or True
Power Supply:
±1.5V to ±7.5V
Supply Current:
3mA
Input Impedance:
25MΩ @ 1kHz
Output Impedance:
250Ω @ 1kHz
Gain 1x (G1)
Bandwidth:
0Hz – 1MHz
Dimensions:
0.75L x 0.46W x 0.06D inches
(19.1L x 11.7W x 1.5D mm)
Weight:
0.017oz
(0.49g)
Description:
An 8 channel op-amp based headstage amplifier containing eight input pins tied to a unity gain (1x) amplifier designed to
record from high impedance electrodes.
Male
Catalog Name:
CON/8o25m-9P
Input Connector:
Female
Catalog Name:
CON/8o25f-9P
Catalog Name:
HSC/8
www.plexon.com
Page 15
A single unity gain (1x) buffered ground pin that is used differentially at the preamplifier level.
Item #:
HST/8o25-GEN2-9P-G1-GR
99036-020
Guide Pin Hole
1 – Channel 1 Out
2 – Channel 2 Out
3 – Channel 3 Out
4 – Channel 4 Out
5 – Channel 5 Out
6 – Channel 6 Out
7 – Channel 7 Out
8 – Channel 8 Out
9 – Ground
10 – (+5V)
11 – (-5V)
12 – Ground
Guide Pin Hole
Guide Pin
9 – Ground
8 – Channel 8 In
OUTPUT
7 – Channel 7 In
6 – Channel 6 In
5 – Channel 5 In
4 – Channel 4 In
3 – Channel 3 In
2 – Channel 2 In
1 – Channel 1 In
Guide Pin
INPUT
This Ground is unity gain (1x) amplified and output from the headstage (pin 9 on the output connector).
S
True Reference (TR)
A single pin is tied to a unity gain (1x) input for a reference electrode pin that is used differentially at the preamplifier
level.
Item #:
HST/8o25-GEN2-9P-G1-TR
TBD
Guide Pin Hole
1 – Channel 1 Out
2 – Channel 2 Out
3 – Channel 3 Out
4 – Channel 4 Out
5 – Channel 5 Out
6 – Channel 6 Out
7 – Channel 7 Out
8 – Channel 8 Out
9 – Reference
10 – (+5V)
11 – (-5V)
12 – Ground
Guide Pin Hole
Guide Pin
9 – Reference
8 – Channel 8 In
OUTPUT
7 – Channel 7 In
6 – Channel 6 In
5 – Channel 5 In
4 – Channel 4 In
3 – Channel 3 In
2 – Channel 2 In
1 – Channel 1 In
Guide Pin
INPUT
S
input of the preamplifier, “REF2” if it is plugged into the channel 9-16 input of the preamplifier.
CAUTION: This input connector provides no ground connection to the animal.
www.plexon.com
Page 16
Headstage D – 8 channel, 0.025” pitch, 10 data pins
Gain 1x (G1)
Channels:
8
Pitch:
0.025”
Data pins:
10
Guide pins:
2
Analog Amplifier,
PBX, PBX2, PBX3
HST/8o25-10P-xR
Gain:
1x
Reference:
Ground or True
Power Supply:
±1.5V to ±7.5V
Supply Current:
3mA
Input Impedance:
40MΩ @ 1kHz
Output Impedance:
240Ω @ 1kHz
Bandwidth:
0Hz – 1MHz
Dimensions:
0.76L x 0.46W x 0.07D inches
(19.3L x 11.7W x 1.8D mm)
Weight:
0.017oz
(0.48g)
Description:
Male
Catalog Name:
CON/8o25m-10P
Input Connector:
Female
Catalog Name:
CON/8o25f-10P
Catalog Name:
HSC/8
www.plexon.com
Page 17
Two pins are connected to a ground line whose output is a unity gain (1x) buffered ground that is used differentially at
the preamplifier level.
Item #:
HST/8o25-10P-GR
50976
Guide Pin Hole
1 – Channel 1 Out
2 – Channel 2 Out
3 – Channel 3 Out
4 – Channel 4 Out
5 – Channel 5 Out
6 – Channel 6 Out
7 – Channel 7 Out
8 – Channel 8 Out
10 – (+5V)
11 – (-5V)
12 – Ground
Guide Pin Hole
Guide Pin
10 – Ground
9 – Ground
8 – Channel 8 In
OUTPUT
7 – Channel 7 In
6 – Channel 6 In
5 – Channel 5 In
4 – Channel 4 In
3 – Channel 3 In
2 – Channel 2 In
1 – Channel 1 In
Guide Pin
INPUT
T
S
True Reference (TR)
One pin is tied to a unity gain (1x) amplifier for a reference electrode that is used differentially at the preamplifier
level, and another pin is tied to ground.
Item #:
HST/8o25-10P-TR
50977
Guide Pin Hole
1 – Channel 1 Out
2 – Channel 2 Out
3 – Channel 3 Out
4 – Channel 4 Out
5 – Channel 5 Out
6 – Channel 6 Out
7 – Channel 7 Out
8 – Channel 8 Out
9 - Reference
10 – (+5V)
11 – (-5V)
12 – Ground
Guide Pin Hole
Guide Pin
10 – Ground
9 – Reference
OUTPUT
8 – Channel 8 In
7 – Channel 7 In
6 – Channel 6 In
5 – Channel 5 In
4 – Channel 4 In
3 – Channel 3 In
2 – Channel 2 In
1 – Channel 1 In
Guide Pin
INPUT
This pin is typically connected to a de-insulated electrode and used as a local reference for spike channels.
S
se this pin to ground the animal via skull screw or cannula, and make sure it touches the cerebrospinal
U
fluid.
www.plexon.com
Page 18
Headstage D2 – 8 channel, 0.025” pitch, 10 data pins
Gain 1x (G1)
Channels:
8
Pitch:
0.025”
Data pins:
10
Guide pins:
2
Analog Amplifier,
PBX, PBX2, PBX3
HST/8o25-GEN210P-G1-xR
Gain:
1x
Reference:
Ground or True
Power Supply:
±1.5V to ±7.5V
Supply Current:
3mA
Input Impedance:
25MΩ @ 1kHz
Output Impedance:
250Ω @ 1kHz
Bandwidth:
0Hz – 1MHz
Dimensions:
0.75L– x 0.46W x 0.06D inches
(19.1L x 11.7W x 1.5D mm)
Weight:
0.017oz
(0.49g)
Description:
An 8 channel op-amp based headstage amplifier containing eight input pins tied to a unity gain (1x) amplifier designed to
Male
Catalog Name:
CON/8o25m-10P
Input Connector:
Female
Catalog Name:
CON/8o25f-10P
Catalog Name:
HSC/8
www.plexon.com
Page 19
Two pins are connected to a ground line whose output is a unity gain (1x) buffered ground that is used
differentially at the preamplifier level.
Item #:
HST/8o25-GEN2-10P-G1-GR
TBD
Guide Pin Hole
1 – Channel 1 Out
2 – Channel 2 Out
3 – Channel 3 Out
4 – Channel 4 Out
5 – Channel 5 Out
6 – Channel 6 Out
7 – Channel 7 Out
8 – Channel 8 Out
10 – (+5V)
11 – (-5V)
12 – Ground
Guide Pin Hole
Guide Pin
10 – Ground
9 – Ground
8 – Channel 8 In
OUTPUT
7 – Channel 7 In
6 – Channel 6 In
5– Channel 5 In
4 – Channel 4 In
3 – Channel 3 In
2 – Channel 2 In
1 – Channel 1 In
Guide Pin
INPUT
T
et the preamplifier reference jumper to “REF1” to use this as a reference if the headstage is plugged into the channel
S
1-8 input of the preamplifier, or “REF2” if it is plugged into the channel 9-16 input of the preamplifier.
True Reference (TR)
level, and another pin is tied to ground.
Item #:
HST/8o25-GEN2-10P-G1-TR
40335-020
Guide Pin Hole
1 – Channel 1 Out
2 – Channel 2 Out
3 – Channel 3 Out
4 – Channel 4 Out
5 – Channel 5 Out
6 – Channel 6 Out
7 – Channel 7 Out
8 – Channel 8 Out
9 - Reference
10 – (+5V)
11 – (-5V)
12 – Ground
Guide Pin Hole
Guide Pin
10 – Ground
9 – Reference
OUTPUT
8 – Channel 8 In
7 – Channel 7 In
6 – Channel 6 In
5 – Channel 5 In
4 – Channel 4 In
3 – Channel 3 In
2 – Channel 2 In
1 – Channel 1 In
Guide Pin
INPUT
et the preamplifier reference jumper to “REF1” to use this as a reference if the headstage is plugged into the channel
S
1-8 input of the preamplifier, or “REF2” if it is plugged into the channel 9-16 input of the preamplifier.
www.plexon.com
Page 20
Headstage E – 16 channel, 0.025” pitch
Gain 1x (G1)
Channels:
16
Analog Amplifier,
PBX, PBX2, PBX3
Pitch:
0.025”
Data pins:
18
Guide pins:
2
HST/16o25-18P-xR
Gain:
1x
Reference:
Ground or True
Power Supply:
±1.5V to ±7.5V
Supply Current:
6mA
Input Impedance:
40MΩ @ 1kHz
Output Impedance:
240Ω @ 1kHz
Dimensions:
0.91L x 0.58W x 0.084D inches
(23.1L x 14.7W x 2.1D mm)
Weight:
0.025oz
(0.72g)
Description:
A 16 channel op-amp based headstage amplifier containing 16 input pins tied to a unity gain (1x) amplifier designed
to record from high impedance electrodes.
Male
Catalog Name:
CON/16o25m-18P
Input Connector:
Female
Catalog Name:
CON/16o25f-18P
Catalog Name:
HSC/16o25
www.plexon.com
Page 21
Two pins are connected to a ground line whose output is a unity gain (1x) buffered ground that is used differentially at
the preamplifier level.
Item #:
HST/16o25-18P-GR
50944
Guide Pin Hole
20 – (+V)
19 – Channel 8 Out
Guide Pin Hole
OUTPUT
(-V) – 11
Ground – 10
Channel 9 Out – 2
Ground –1
Channel 9 In – 2
Channel 10 In – 3
Channel 11 In – 4
Channel 12 In – 5
Channel 13 In – 6
Channel 14 In – 7
Channel 15 In – 8
Channel 16 In – 9
Ground – 10
Guide Pin
11 – Channel 1 In
12 – Channel 2 In
13 – Channel 3 In
14 – Channel 4 In
15 – Channel 5 In
16 – Channel 6 In
17 – Channel 7 In
18 – Channel 8 In
Guide Pin
INPUT
his pin is internally tied to system Ground input pin 10 and is unity gain (1x) amplified and output from the headstage (pin
T
1 on the output connector). We call this output the Buffered Ground.
Set the preamplifier reference jumper to “REF1” or “REF2” to use this signal as a reference.
www.plexon.com
Page 22
True Reference (TR)
level, and 1 pin tied to ground.
Item #:
HST/16o25-18P-TR
50945
Guide Pin Hole
20 – (+V)
Guide Pin Hole
OUTPUT
(-V) – 11
Ground – 10
Channel 9 Out – 2
Reference – 1
Reference –1
Channel 9 In – 2
Channel 10 In – 3
Channel 11 In – 4
Channel 12 In – 5
Channel 13 In – 6
Channel 14 In – 7
Channel 15 In – 8
Channel 16 In – 9
Ground – 10
Guide Pin
11 – Channel 1 In
12 – Channel 2 In
13 – Channel 3 In
14 – Channel 4 In
15 – Channel 5 In
16 – Channel 6 In
17 – Channel 7 In
18 – Channel 8 In
Guide Pin
INPUT
This pin is unity gain (1x) amplified and output from the headstage (pin 1 on the output connector).
Set the preamplifier reference jumper to “REF1” or “REF2” to use this signal as a reference.
www.plexon.com
Page 23
Headstage E2a – 16 channel, 0.025” pitch, 2 Guide Pins
Gain 1x (G1)
Channels:
16
Pitch:
0.025”
Data pins:
18
Guide pins:
2
Analog Amplifier,
PBX, PBX2, PBX3
HST/16o25-GEN218P-2GP-G1
Gain:
1x
Reference:
Ground and True
Power Supply:
±1.5V to ±7.5V
Supply Current:
8mA
Input Impedance
25MΩ @ 1kHz
Output Impedance:
250Ω @ 1kHz
Dimensions:
0.74L x 0.46W x 0.09D inches
(18.8L x 11.7W x 2.3D mm)
Weight:
0.19oz
(0.60g)
Description:
A 16 channel op-amp based headstage amplifier containing 16 input pins tied to a unity gain 1x amplifier designed to
Male
Catalog Name:
CON/16o25m-18P
Input Connector:
Female
Catalog Name:
CON/16o25f-18P
Catalog Name:
HSC/16o25-GEN2
www.plexon.com
Page 24
Ground and True Reference (GR)
Channels 1-16 of the G1x amplifiers are designed for recording from high-impedance recording electrodes. The 17th
channel may be used to generate a Reference signal from a de-insulated low-impedance electrode. This Reference
signal can be used differentially at the preamplifier level to subtract common mode noise and artifacts from the 16
recording channels. The headstage’s unity gain amplifier generates a Buffered Ground signal that may also be used
differentially at the preamplifier level (in place of the Reference signal).
Item #:
HST/16o25-GEN2-18P-2GP-G1
40684-020
19 – Channel 6
18 – Channel 5
17 – Channel 4
16 – Channel 3
15 – Channel 2
14 – Channel 1
13 – (-V)
Channel 13 – 7
Channel 12 – 6
Channel 11 – 5
Channel 10 – 4
Channel 9 – 3
Reference – 2
Guide Pin Hole
20 – Channel 7
Channel 14 – 8
Ground – 1
21 – Channel 8
Channel 15 – 9
22 – (+V)
18 – Channel 8
Channel 16 – 9
Channel 16 – 10
17 – Channel 7
Channel 15 – 8
Guide Pin Hole
16 – Channel 6
Channel 14 – 7
Ground –12
15 – Channel 5
Channel 13 – 6
14 – Channel 4
Channel 12 – 5
Guide Pin
13 – Channel 3
Ground – 10
12 – Channel 2
Channel10 – 3
11 – Channel 1
Channel 9 – 2
OUTPUT
Channel 11 – 4
Guide Pin
Reference – 1
INPUT
Set the preamplifier reference jumper to “REF2” to use this signal as a reference.
T
www.plexon.com
Page 25
Headstage E2b – 16 channel, 0.025” pitch, 6 Guide Pins
Gain 1x (G1)
Channels:
16
Pitch:
0.025”
Data pins:
18
Guide pins:
6
Analog Amplifier,
PBX, PBX2, PBX3
HST/16o25-GEN218P-6GP-G1
Gain:
1x
Reference:
Ground and True
Power Supply:
±1.5V to ±7.5V
Supply Current:
8mA
Input Impedance:
25MΩ @ 1kHz
Output Impedance:
250Ω @ 1kHz
Dimensions:
0.74L x 0.46W x 0.09D inches
(18.8L x 11.7W x 2.3D mm)
Weight:
0.022oz
(0.60g)
Description:
Input Connector Information: Connectors #6 and 7 (Nickel-free)
Input Mating Connector 6:
Male
Catalog Name:
CON/16m-V
Input Connector 6:
Female
Catalog Name:
CON/16f-V
Male
Catalog Name:
CON/16m-V-NiFree
(MRI Compatible)
Catalog Name:
HSC/16o25-GEN2
www.plexon.com
Page 26
Ground and True Reference
channel may be used to generate a reference signal from a de-insulated low-impedance electrode. This reference
Item #:
HST/16o25-GEN2-18P-6GP-G1
33504
Channel 9 Out – 3
Guide Pin Hole
Ground – 1
13 – (-V)
Reference – 2
22 – (+V)
18 – Channel 8 In
Guide Pin Hole
17 – Channel 7 In
Channel 16 In – 9
Ground – 12
16 – Channel 6 In
Channel 15 In – 8
15 – Channel 5 In
Channel 14 In – 7
Guide Pin
14 – Channel 4 In
Channel 13 In – 6
Guide Pin
13 – Channel 3 In
Channel 12 In – 5
Guide Pin
12 – Channel 2 In
Channel 11 In – 4
Ground – 10
11 – Channel 1 In
Channel 10 In – 3
Guide Pin
OUTPUT
Channel 9 In – 2
Guide Pin
Guide Pin
Reference – 1
INPUT
T
www.plexon.com
Page 27
Headstage F – 32 channel, 0.025” pitch
Gain 1x (G1)
Channels:
32
Pitch:
0.025”
Data pins:
36
Guide pins:
4
Analog Amplifier,
PBX, PBX2, PBX3
HST/32o25-36P-xR
Gain:
1x
Reference:
Ground or True
Power Supply:
±1.5V to ±7.5V
Supply Current:
12mA
Input Impedance:
40MΩ @ 1kHz
Output Impedance:
240Ω @ 1kHz
Dimensions:
1.42L x 0.73W x 0.09D inches
(36.1L x 18.5W x 2.3D mm)
Weight:
0.065oz
(1.84g)
Description:
A 32 channel op-amp based headstage amplifier containing 32 input pins tied to a unity gain (1x) amplifier designed
to record from high impedance electrodes.
Input Connector Information: Connectors #8 and #9 (Nickel-free)
Male
Catalog Name:
CON/32m-V
Input Connector 8:
Female
Catalog Name:
CON/32f-V
Male
Catalog Name:
CON/32m-V-NiFree
(MRI Compatible)
Catalog Name:
HSC/32o25
www.plexon.com
Page 28
Four pins are connected to a ground line whose output is a unity gain (1x) buffered ground that is used differentially
at the preamplifier level.
Item #:
HST/32o25-36P-GR
50966
Guide Pin Hole
38 – Ground
Guide Pin Hole
OUTPUT
(-5V) – 20
Buffered Ground –19
Channel 7 Out – 9
Channel 6 Out – 8
Channel 5 Out – 7
Channel 4 Out – 6
Channel 3 Out – 5
Channel 2 Out – 4
Channel 1 Out – 3
Ground – 2
(+SV) – 1
Ground –1
Channel 17 In – 2
Channel 18 In – 3
Channel 19 In – 4
Channel 20 In – 5
Channel 21 In – 6
Channel 22 In – 7
Channel 23 In – 8
Channel 24 In – 9
Ground – 18
Guide Pin
19 – Ground
20 – Channel 1 In
21 – Channel 2 In
22 – Channel 3 In
23 – Channel 4 In
24 – Channel 5 In
25 – Channel 6 In
26 – Channel 7 In
27 – Channel 8 In
28 – Channel 9 In
29 – Channel 10 In
36 – Ground
Guide Pin
INPUT
his pin is internally tied to system ground input pins 18 and 19 and is unity gain (1x) amplified and output from the
T
headstage (pin 19 on the output connector). We call this output the Buffered Ground.
The signal is output to the channel 1-16 branch (the red color-coded branch) of the headstage cable.
Set the channels 1-16 preamplifier reference jumper to “REF1” to use this signal as a reference.
his pin is internally tied to system ground input pin 18 and 19 and is unity gain (1x) amplified and output from the
T
headstage (pin 21 on the output connector). We call this output the Buffered Ground.
The signal is output to the channels 17-32 branch (the yellow color-coded branch) of the headstage cable.
Input Pin 18 and 19: Ground
Use these pins to ground the animal via skull screw or cannula, and make sure it touches the cerebrospinal fluid.
hese pins are internally tied to input pins 1 and 36, and tied to the system ground on Input Pin 18 and 19: Ground for
T
Ground Reference only.
www.plexon.com
Page 29
True Reference (TR)
Two pins are tied to a unity gain (1x) amplifier for a reference electrode that are each used differentially at the
preamplifier level (one reference per 16 channels), and two pins tied to ground.
HST/32o25-36P-TR
50968
Reference #2 – 1
Channel 17 In – 2
Channel 18 In – 3
Channel 19 In – 4
Channel 20 In – 5
Channel 21 In – 6
Channel 22 In – 7
Channel 23 In – 8
Channel 24 In – 9
Ground – 18
OUTPUT
Guide Pin Hole
38 – Ground
21 – Reference #2
Guide Pin Hole
Guide Pin
19 – Ground
20 – Channel 1 In
21 – Channel 2 In
22 – Channel 3 In
23 – Channel 4 In
24 – Channel 5 In
25 – Channel 6 In
26 – Channel 7 In
27 – Channel 8 In
28 – Channel 9 In
36 – Reference #1
Guide Pin
INPUT
(-5V) – 20
Reference #1 – 19
Channel 7 Out – 9
Channel 6 Out – 8
Channel 5 Out – 7
Channel 4 Out – 6
Channel 3 Out – 5
Channel 2 Out – 4
Channel 1 Out – 3
Ground – 2
(+5V) – 1
Item #:
The signal is output to the channel 1-16 branch (the red color-coded branch) of the headstage cable.
The signal is output to the channels 17-32 branch (the yellow color-coded branch) of the headstage cable.
Use these pins to ground the animal via skull screw or cannula, and make sure it touches the cerebrospinal fluid.
These pins are tied to the system ground.
www.plexon.com
Page 30
Headstage F3 – 32 channel, 0.025” pitch
Gain 1x (G1)
Channels:
32
Pitch:
0.025”
Data pins:
36
Guide pins:
4
Analog Amplifier,
PBX, PBX2, PBX3
HST/32o25-GEN336P-G1
Dimensions:
0.85L x 0.56W x 0.10D inches
(21.5L x 14.3W x 2.5D mm)
Gain:
1x
Reference:
Ground and True
Power Supply:
±1.5V to ±7.5V
Supply Current:
8mA
Input Impedance:
25MΩ @ 1kHz
Output Impedance:
250Ω @ 1kHz
Weight:
0.04oz
(1.0g)
Description:
Male
Catalog Name:
CON/32m-V
Input Connector 8:
Female
Catalog Name:
CON/32f-V
Male
Catalog Name:
CON/32m-V-NiFree
(MRI Compatible)
Catalog Name:
HSC/16o25-GEN2
www.plexon.com
Page 31
Channels 1-32 of the G1x amplifiers are designed for recording from high-impedance recording electrodes. The 33rd
and 34th channel may be used to generate reference signals from de-insulated low-impedance electrodes. These
reference signals can be used differentially at the preamplifier level to subtract common mode noise and artifacts from
the 32 recording channels. The headstage’s unity gain (1x) amplifiers generate Buffered Ground signals that may also
be used differentially at the preamplifier level (in place of the Reference signal).
Item #:
HST/32o25-GEN3-36P-G1
33499
Guide Pin Hole
40 – Reference #1
38 – hannel 15 Out
23 – Buffered Ground #1
22 – Ground
Guide Pin Hole
OUTPUT
(-V) – 21
Ground – 20
Buffered Ground #2 – 19
18Channel 32 Out – 18
Reference #2 – 2
(+V) – 1
Guide Pin
Reference #2 – 1
Channel 17 In – 2
Channel 18 In – 3
Channel 19 In – 4
Channel 20 In – 5
Channel 21 In – 6
Channel 22 In – 7
Channel 23 In – 8
Channel 24 In – 9
Ground – 18
Guide Pin
Guide Pin
19 – Ground
20 – Channel In – 1
36 – Reference #1
Guide Pin
INPUT
1x amplified reference line.
eference #1 is output from the headstage on pin 40, and is sent to the preamplifer via the headstage cable branch that
R
connects to channels 1-16 (red branch).
It is recommended that you connect the two reference inputs to a single reference electrode during the construction of
your electrode implant. Otherwise, channels 1-16 will be using a different reference than 17-32.
Set the preamplifer reference jumper to “REF2” to use this signal as a reference.
If a reference electrode is not being used, connect input pin 36 to input pin 18 or 19 ground to avoid having it float.
eference #2 is output from the headstage on pin 2, and is sent to the preamplifer via the headstage cable branch that
R
connects to channels 17-32 (yellow branch).
It is recommended that you connect the two reference inputs to a single reference wire during the construction of your
array. Otherwise, channels 1-16 will be using a different reference than 17-32.
If a reference electrode is not being used, connect input pin 1 to input pin 18 or 19 ground to avoid having it float.
www.plexon.com
Page 32
Input Pins 18 and 19: Ground (output pin 19 and 23)
ins 18 and 19 are tied together in the HST/32V-G20 headstage. Connect either pin 18 or pin 19 to a scull screw or
P
cannula that is in contact with the cerebrospinal fluid. For added safety, it is advised to connect pin 18 to the skull screw
AND pin 19 to a second scull screw, or both pin 18 and 19 to the cannula.
his Ground is unity gain (1x) amplified and output from the headstage on pins 23 and 19. Buffered Ground #1 is sent
T
through the headstage cable branch for Channels 1-16, and Buffered Ground #2 is sent through the headstage cable
branch for Channels 17-32.
Set the preamplifer reference jumper on each board to “REF1” to use Buffered Ground as the reference.
NOTE: Plexon MAP preamplifiers are most often configured with separate spike and field potential boards.
Each board is equipped with jumpers that can be used to select a reference. For example, you can use the
“REF2” jumper on the spike board for a local reference, and “REF1” on the field potential board to use the
Buffered Ground signal as a distant reference.
The most common reference configuration for the HST/32o25-GEN3-36P-G1 is to tie both Reference #1 and
Reference #2 together so that each branch of the headstage cable is sending the same reference signal to
each preamplifier board.
www.plexon.com
Page 33
Headstage H – 16 channel, 0.025” pitch, VLSI
Gain 20x (G20) –
2 lavender dots in center
Channels:
16
Pitch:
0.025”
Data pins:
18
Guide pins:
6
Analog Amplifier,
PBX, PBX2, PBX3
HST/16V-G20
Gain:
20x
Reference:
Fully differential
Power Supply:
±2.25V to ±2.75V
Supply Current:
6.8mA to 8.5mA
Input Impedance:
22MΩ @ 1kHz
Output Impedance:
158Ω @ 1kHz
Bandwidth (-3dB):
0.8kHz to 54kHz
RMS noise with inputs grounded:
6.2µV RMS for DC-10kHz
Dimensions:
0.94L x 0.42W x 0.10D inches
(23.9L x 10.7W x 2.5D mm)
Weight:
0.036oz
(1.03g)
Description:
A 16 channel miniature Very Large Scale Integration (VLSI) based headstage amplifier designed to record from highimpedance electrodes, containing 16 channel input pins tied to 20x gain amplifiers and one reference input tied to a
unity gain (1x) amplifier.
Input Connector Information: Connector #6 and #7 (Nickel-free)
Male
Catalog Name:
CON/16m-V
Input Connector:
Female
Catalog Name:
CON/16f-V
Male
Catalog Name:
CON/16m-V-NiFree
(MRI Compatible)
Catalog Name:
HSC/16V
www.plexon.com
Page 34
channel may be used to generate a reference signal from a de-insulated low-impedance electrode. This reference
Item #:
HST/16V-G20
52242
Guide Pin Hole
22 – (+V)
13 – Reference
Guide Pin Hole
OUTPUT
Ground – 12
(-V) – 10
Channel 8 Out – 9
Channel 7 Out – 8
Channel 6 Out – 7
Channel 5 Out – 6
Channel 4 Out – 5
Channel 3 Out – 4
Channel 2 Out – 3
Channel 1 Out – 2
Ground – 1
Guide Pin
Reference – 1
Channel 9 In – 2
Channel 10 In – 3
Channel 11 In – 4
Channel 12 In – 5
Channel 13 In – 6
Channel 14 In – 7
Channel 15 In – 8
Channel 16 In – 9
Ground – 10
Guide Pin
Guide Pin
Guide Pin
11 – Channel 1 In
12 – Channel 2 In
13 – Channel 3 In
14 – Channel 4 In
15 – Channel 5 In
16 – Channel 6 In
17 – Channel 7 In
18 – Channel 8 In
Guide Pin
Guide Pin
INPUT
Set the preamplifier reference jumper to “REF2” to use this signal as the reference.
If a reference electrode is not being used, connect pin 1 to pin 10 to avoid having it float.
his Ground is unity gain (1x) amplified and output from the headstage (pin 11 on output connector). We call this output
T
the Buffered Ground.
Set the preamplifier reference jumper to “REF1” to use Buffered Ground as the reference.
NOTE: Plexon preamplifiers are most often configured with separate spike and field potential boards. Each board is
equipped with jumpers that can be used to select a reference. For example, if you have a low impedance reference
electrode connected to input pin 1, you can use the “REF2” jumper on the spike board for a local reference, and
“REF1” on the field potential board to use the Buffered Ground signal as a distant reference.
CAUTION: The Plexon VLSI headstage operates with a ±2.5 Volt power supply. Connecting it to a ±5 Volt power supply
will cause IMMEDIATE and IRREPARABLE damage to the headset. For more information, please reference the CAUTION
section on the last page of the Headstage J section.
www.plexon.com
Page 35
Headstages J – 32 channel, 0.025” pitch, VLSI
Gain 20x (G20) –
Channels:
32
Pitch:
0.025”
Data pins:
36
Guide pins:
4
Analog Amplifier,
PBX, PBX2, PBX3
HST/32V-G20
Gain:
20x
Reference:
Ground and True
Power Supply:
±2.25V to ±2.75V
Supply Current:
6.8mA to 8.5mA
Input Impedance:
22MΩ @ 1kHz
Output Impedance:
158Ω @ 1kHz
Bandwidth (-3dB):
0.8kHz to 54kHz
RMS noise with inputs grounded:
6.2µV RMS for DC-10kHz
Dimensions:
0.85L x 0.59W x 0.15D inches
(21.6L x 15.0W x 3.8D mm)
Weight:
0.061oz
(1.73g)
Description:
A 32 channel miniature Very Large Scale Integration (VLSI) based headstage amplifier designed to record from
high-impedance electrodes, containing 32 channel input pins tied to 20x gain amplifiers and two reference inputs tied
to unity gain (1x) amplifiers.
Male
Catalog Name:
CON/32m-V
Input Connector 8:
Female
Catalog Name:
CON/32f-V
Male
Catalog Name:
CON/32m-V-NiFree
(MRI Compatible)
Catalog Name:
HSC/32V
www.plexon.com
Page 36
Channels 1-32 of the G20x amplifiers are designed for recording from high-impedance recording electrodes. The 33rd
and 34th channel may be used to generate reference signals from de-insulated low-impedance electrodes. These
reference signals can be used differentially at the preamplifier level to subtract common mode noise and artifacts from
the 32 recording channels. The headstage’s unity gain (1x) amplifiers generate Buffered Ground signals that may also
be used differentially at the preamplifier level (in place of the Reference signal).
Item #:
HST/32V-G20
52244
Guide Pin Hole
40 – (-2.5V)
39 – Reference #1
22 – Reference #2
Guide Pin Hole
OUTPUT
Ground – 21
Channel 7 Out – 9
Channel 6 Out – 8
Channel 5 Out – 7
Channel 4 Out – 6
Channel 3 Out – 5
Channel 2 Out – 4
Channel 1 Out – 3
(+2.5V) – 2
Ground – 1
Guide Pin
Reference #2 – 1
Channel 17 In – 2
Channel 18 In – 3
Channel 19 In – 4
Channel 20 In – 5
Channel 21 In – 6
Channel 22 In – 7
Channel 23 In – 8
Channel 24 In – 9
Ground – 18
Guide Pin
Guide Pin
19 – Ground
20 – Channel 1 In
21 – Channel 2 In
22 – Channel 3 In
23 – Channel 4 In
24 – Channel 5 In
25 – Channel 6 In
26 – Channel 7 In
27 – Channel 8 In
28 – Channel 9 In
36 – Reference #1
Guide Pin
INPUT
eference #1 is output from the headstage on pin 39, and is sent to the preamplifier via the headstage cable branch that
R
connects to channels 1-16 (red branch).
It is recommended that you connect the two reference inputs to a single reference electrode during the construction of your
electrode implant. Otherwise, channels 1-16 will be using a different reference than 17-32.
If a reference electrode is not being used, connect input pin 36 (Reference #1) to input pin 18 or 19 (Ground) to avoid
having it float.PP
eference #2 is output from the headstage on pin 22, and is sent to the preamplifier via the headstage cable branch that
R
connects to channels 17-32 (yellow branch).
It is recommended that you connect the two reference inputs to a single reference wire during the construction of your
array. Otherwise, channels 1-16 will be using a different reference than 17-32.
If a reference electrode is not being used, connect input pin 1 (Reference #2) to input pin 18 or 19 (Ground) to avoid
having it float.
www.plexon.com
Page 37
Input Pins 18 and 19: Ground (output pin 11 and 20)
ins 18 and 19 are tied together in the HST/32V-G20 headstage. Connect either pin 18 or pin 19 to a scull screw or
P
cannula that is in contact with the cerebrospinal fluid. For added safety, it is advised to connect pin 18 to a skull screw AND
pin 19 to a second scull screw, or both pin 18 and 19 to the cannula.
his Ground is unity gain (1x) amplified and output from the headstage on pins 11 (Buffered Ground #1) and 20 (Buffered
T
Ground #2). Buffered ground #1 is sent through the headstage cable branch for channels 1-16, and Buffered Ground #2 is
sent through the headstage cable branch for channels 17-32.
Set the preamplifier reference jumper on each board to “REF1” to use Buffered Ground as the reference.
NOTE: Plexon preamplifiers are most often configured with separate spike and field potential boards. Each
board is equipped with jumpers that can be used to select a reference. For example, you can use the “REF2”
jumper on the spike board for a local reference, and “REF1” on the field potential board to use the
Buffered Ground signal as a distant reference.
The most common reference configuration for the HST/32V-G20 is to tie both Reference #1 and Reference #2
together so that each branch of the headstage cable is sending the same reference signal to each preamplifier
board.
CAUTION: The Plexon VLSI headstages listed below operate with a ±2.5 Volt power supply. Connecting these
to a ±5 Volt power supply will cause IMMEDIATE and IRREPARABLE damage to the headset:
HST/16V-G20 (with or without LEDs or latching)
HST/32V-G20 (with or without LEDs or latching)
Plexon preamplifiers supply either ±2.5 Volts or ±5 Volts to the headset. The voltage level supplied by the
preamplifier is indicated by the color of the power indicator LEDs on the front of the preamplifier as
illustrated below. Green LEDs indicate ±2.5 Volts supplied to the headset. Red LEDs indicate ±5 Volts
supplied to the headset.
IMPORTANT: Verify the voltage level before connecting the headset to the preamplifier.
Green LEDs indicate ±2
Volts supplied to headset
Red LEDs indicate ±5 Volts
supplied to headset
DO NOT CONNECT ANY OF THE
HEADSETS LISTED ABOVE
www.plexon.com
Page 38
Headstage M – 96 channel, 0.050” pitch
Gain 20x (G20) –
Channels:
96
Pitch:
0.050”
Data pins:
108
Guide pins:
No guide pins
Analog Amplifier,
PBX, PBX2, PBX3
HST/CereStage 96
Gain:
1x
Reference:
Dedicated reference
input
(with optional buffered
ground reference)
Power Supply:
±1.5V to ±7.5V
Supply Current:
8mA
Input Impedance:
25MΩ @ 1kHz
Output Impedance:
250Ω @ 1kHz
Dimensions:
1.1L x 1.55W x 0.43D inches
(27.9L x 39.4W x 10.9D mm)
Weight:
0.28oz
(8.05g)
Description:
A 96 channel headstage amplifier containing 96 input pins tied to a unity gain 1x amplifier designed to record from
high impedance electrodes, for connecting to a Utah array with an ICS style connector.
Male
Catalog Name:
3x Mill-Max 852-10-036-10-001000
Input Connector:
Female
Catalog Name:
3x Samtec SFMC-118-L2-S-D
Catalog Name:
3
x HSC/CereStageTM 96-2H26F-36G
(2 x 26 pin Harwin output connector)
for use with 16 to 64 MiniDigi™ Digitizing
Amplifiers, analog OmniPlex System
amplifier or any Multichannel Acquisition
Processor (MAP) Data Acquisition System
preamplifier.
www.plexon.com
Page 39
Channel 84 In – 9
Channel 86 In – 8
Channel 88 In – 7
Channel 90 In – 6
Channel 92 In – 5
Channel 94 In – 4
Channel 96 In – 3
Ground – 2
No Connection – 1
20 – Ground
19 – Ground
Channel 52 In – 9
Channel 54 In – 8
Channel 56 In – 7
Channel 58 In – 6
Channel 60 In – 5
Channel 62 In – 4
Channel 64 In – 3
Ground – 2
No Connection – 1
20 – Reference #1
IChannel 12 In – 13
Channel 20 In – 9
Channel 22 In – 8
Channel 24 In – 7
Channel 26 In – 6
Channel 28 In – 5
Channel 30 In – 4
Channel 32 In – 3
Ground – 2
(-V) – 21
Ground – 20
Channel 8 Out – 6
Channel 6 Out – 5
Channel 4 Out – 4
Channel 2 Out – 3
Reference* – 2
(+V) – 1
Guide Pin Hole
40 – Reference*
22 – Ground
Guide Pin Hole
(-V) – 21
Ground – 20
Reference* – 2
(+V) – 1
32 – Channel 9 In
Guide Pin Hole
40 – Reference*
22 – Ground
Guide Pin Hole
33 – Channel 7 In
Channel 8 In – 15
(-V) – 21
Ground – 20
Reference* – 2
(+V) – 1
IChannel 38 In – 16
34 – Channel 5 In
Channel 6 In – 16
19 – Ground
35 – Channel 3 In
Channel 4 In – 17
Guide Pin Hole
40 – Reference*
22 – Ground
Guide Pin Hole
19 – Ground
20 – Reference #2
Item #:
No Connection – 1
36 – Channel 1 In
Channel 2 In – 18
Dedicated Reference Input (with optional buffered ground reference)
Seven pins are connected to a ground line whose output is a unity gain buffered ground that is used differentially at
the preamplifier level. Two pins for reference electrodes can change via jumper on headstage.
HST/CereStage 96
40694
INPUT
OUTPUT
*Output Reference is set by the jumper, see next page for details.
www.plexon.com
Page 40
Input Pin 20 on input connector for channels 65-96: Reference #2
This Reference is only passed to the preamplifer when the headstage reference jumper is set to “R2”.
eference #2 is output (when selected on the headstage jumper) from pins 2 and 40 on each of the output connectors,
R
and is sent to each of the preamplifer board’s input pin 26 via the headstage cable.
Input Pins 2 and 19 on input connectors for channels 1-32 and 65-96, and input pins 2, 19 and 20 on input connector for channels 33-64: Ground
his Ground is unity gain (1x) amplified and output from the headstage on pin 19 and 23 on each of the output connectors,
T
he input ground pins are tied together, but each buffered ground output is going through its own unity gain amplifier.
T
Connect at least one ground pin to a scull screw or cannula that is in contact with the cerebrospinal fluid. For added safety,
it is advised to connect more ground pins to the skull screw and/or cannula.
Set the preamplifer reference jumper on each board to “REF1” to use Buffered Ground as the reference.
Input Pin 20 on input connector for channels 1-32: Reference #1
This Reference is only passed to the preamplifer when the headstage reference jumper is set to “R1”.
eference #1 is output (when selected on the headstage jumper) from pins 2 and 40 on each of the output connectors,
R
www.plexon.com
Page 41
Headstage N – 8 channel, 0.049” pitch (Biopotential) - PNS
Channels:
8
Pitch:
0.049”
Data pins:
20
Guide pins:
N/A
Analog Amplifier,
PBX, PBX2, PBX3
HST/8-BIOP-PNS
Gain:
50x
Reference:
Fully differential
Power Supply:
±2.5V to ±15V
Input Impedance:
53MΩ @ 1kHz
Output Impedance:
37MΩ @ 1kHz
Dimensions:
1.40L x 0.91W x 0.20D inches
(35.5L x 23.1W x 5.0D mm)
Weight:
0.127oz
3.6g
Description:
An 8 channel biopotential headstage for peripheral nerve recording with a 20 pin male Gecko input connector, eight
pairs of differential inputs, 120Hz 2 pole to 12kHz 1 pole filtering and 50x gain.
Male
Catalog Name:
Gecko G125-MV12005L0P
Input Connector:
Female
Catalog Name:
Gecko G125-FV12005L0P
Catalog Name:
HSC/8-BIOP
This cable is for
use with the 8
channel PNS or
EDIF biopotential
headstages.
www.plexon.com
Page 42
Catalog name:
Item #:
HST/8-BIOP-PNS
33606-008
4 – Channel 7+ In
3 – Channel 8+ In
Channel -8 In – 13
1 – (V+)
3 – Channel 7 Out
5 – Channel 5 Out
7 – Channel 3 Out
9 – Channel 1 Out
11 – (V=)
5 – Channel 6+ In
Ground – 2
Channel 8 Out – 4
Channel 6 Out – 6
Channel 4 Out – 8
Ground – 12
6 – Channel 5+ In
1 – Ground
7 – Channel 4+ In
2 – N/C
8 – Channel 3+ In
N/C - 11
9 – Channel 2+ In
Ground – 12
10 – Channel 1+ In
OUTPUT
INPUT
Use this pin to ground the animal.
Set the preamp to REF1 (PRA/PRA1/PRA3) or REFA (OmniPlex®/OmniPlex D) to use this ground as a reference at the
preamp level
when it is plugged into the Channel 1-8 input of the preamplifier.
the preamp to REF2 (PRA/PRA2/PRA3) or REFB (OmniPlex/OmniPlex D) to use this ground as a reference at the
Set
preamp level when it is plugged into the Channel 9-16 input of the preamplifier.
www.plexon.com
Page 43
Headstage P – 8 channel, 0.049” pitch (Biopotential) - EDIF
Channels:
8
Pitch:
0.049”
Data pins:
20
Guide pins:
N/A
Analog Amplifier,
PBX, PBX2, PBX3
HST/8-BIOP-EDIF
Gain:
10x
Reference:
Fully differential
Power Supply:
±2.5V to ±15V
Input Impedance:
26MΩ @ 1kHz
Output Impedance:
53MΩ @ 1kHz
Dimensions:
1.40L x 0.91W x 0.20D inches
(35.5L x 23.1W x 5.0D mm)
Weight:
0.127oz
3.6g
Description:
An 8 channel biopotential headstage for EMG, EEG, EKG recording with a 20 pin male Gecko input connector, eight
pairs of differential inputs, 0.2Hz 1 pole to 2kHz 1 pole filtering and 10x gain.
Male
Catalog Name:
Gecko G125-MV12005L0P
Input Connector:
Female
Catalog Name:
Gecko G125-FV12005L0P
Catalog Name:
HSC/8-BIOP
This cable is for
use with the 8
channel PNS or
EDIF biopotential
headstages.
www.plexon.com
Page 44
Catalog name:
Item #:
HST/8-BIOP-EDIF
33621-001
4 – Channel 7+ In
3 – Channel 8+ In
1 – (V+)
3 – Channel 7 Out
5 – Channel 5 Out
7 – Channel 3 Out
9 – Channel 1 Out
11 – (V=)
5 – Channel 6+ In
Ground – 2
Channel 8 Out – 4
Channel 6 Out – 6
Channel 4 Out – 8
Ground – 12
6 – Channel 5+ In
1 – Ground
7 – Channel 4+ In
2 – N/C
8 – Channel 3+ In
N/C - 11
9 – Channel 2+ In
Ground – 12
10 – Channel 1+ In
OUTPUT
INPUT
Use this pin to ground the animal.
Set the preamp to REF1 (PRA/PRA3/PRA3) or REFA (OmniPlex/OmniPlex D) to use this ground as a reference at the
preamp level
when it is plugged into the Channel 1-8 input of the preamplifier.
the preamp to REF2 (PRA/PRA2/PRA3) or REFB (OmniPlex/OmniPlex D) to use this ground as a reference at the
Set
preamp level when it is plugged into the Channel 9-16 input of the preamplifier.
www.plexon.com
Page 45
Headstage Q – 16 channel, 0.025” pitch (Digital)
Channels:
16
Headstage Type: Digital
Pitch:
0.025”
Data pins:
17
Digital Headstage Processor (DHP)
Guide pins:
6
HST/16D
Gain:
N/A
Reference:
User selectable
Power Supply:
N/A
Input Impedance:
13MΩ @ 1kHz
Output Impedance:
N/A
Dimensions:
0.97L x 0.52W x 0.10D inches
(24.6L x 13.2W x 2.5D mm)
Weight:
0.032oz
(0.90g)
Description:
A 16 channel digital headstage amplifier designed to record from high-impedance electrodes, containing 16 channel
input pins and one reference input.
Input Connector Information: Connectors #6 and 7 (Nickel-free)
Male
Catalog Name:
CON/16m-V
Input Connector 6:
Female
Catalog Name:
CON/16f-V
Male
Catalog Name:
CON/16m-V-NiFree
(MRI Compatible)
Catalog Name:
HSC/DHSC1
This cable is for
use with the 16
and 32 channel
Digital Headstages.
www.plexon.com
Page 46
User Selectable Reference
Channels 1-16 are designed for recording from high-impedance recording electrodes. The 17th channel may be used
to generate a reference signal from a de-insulated low-impedance electrode. This reference signal can be used to
subtract common mode noise and artifacts from the 16 recording channels.
Catalog name:
Item #:
HST/16D
31012
16 – Channel 6 In
17 – Channel 7 In
18 – Channel 8 In
Channel 15 In – 8
Channel 16 In – 9
12 – NC
11 – CS+
10 – SCLK+
9 – MOSI+
8 – MISO+
15 – Channel 5 In
Channel 14 In – 7
Ground – 7
NC – 6
–CS – 5
–SCLK – 7
–MOSI – 3
–MISO – 2
+V – 1
14 – Channel 4 In
Channel 13 In – 6
Guide Pin
13 – Channel 3 In
Channel 12 In – 5
Guide Pin
12 – Channel 2 In
Channel 11 In – 4
Ground – 10
Guide Pin
11 – Channel 1 In
Channel 10 In – 3
Guide Pin
OUTPUT
Channel 9 In – 2
Guide Pin
Guide Pin
Reference – 1
INPUT
Input Pin 1: Reference or Ground
This input can be used as a dedicated reference electrode or ground, depending on how it is set in OmniPlex Server.
the reference option in OmniPlex Server to “True Reference” to use this input as a reference. It is typically connected
Set
to a de-insulated electrode and used as a local reference for spike channels.
Set the reference option in OmniPlex Server to “grounded reference” to connect this input to ground. This is typically used
as a distant reference for field potentials, and also for spike channels.
www.plexon.com
Page 47
Headstage R – 32 channel, 0.025” pitch (Digital)
Channels:
32
Headstage Type: Digital
Pitch:
0.025”
Data pins:
33
Digital Headstage Processor (DHP)
Guide pins:
4
HST/32D
Gain:
N/A
Reference:
User selectable
Power Supply:
N/A
Input Impedance:
13MΩ @ 1kHz
Output Impedance:
N/A
Dimensions:
0.97L x 0.52W x 0.10D inches
(24.6L x 13.2W x 2.5D mm)
Weight:
0.035oz
(1.0g)
Description:
A 32 channel digital headstage amplifier designed to record from high-impedance electrodes, containing 32 channel
input pins and one reference input.
Male
Catalog Name:
CON/32m-V
Input Connector 8:
Female
Catalog Name:
CON/32f-V
Male
Catalog Name:
CON/32m-V-NiFree
(MRI Compatible)
Catalog Name:
HSC/DHSC1
This cable is for
use with the 16
and 32 channel
Digital Headstages.
www.plexon.com
Page 48
User Selectable Reference
Channels 1-32 are designed for recording from high-impedance recording electrodes. The 33rd channel may be used
to generate reference signals from de-insulated low-impedance electrodes. These reference signals can be used
differentially to subtract common mode noise and artifacts from the 32 recording channels.
HST/32D
91710
Guide Pin
Ground - 1
Channel 17 In – 2
Channel 18 In – 3
Channel 19 In – 4
Channel 20 In – 5
Channel 21 In – 6
Channel 22 In – 7
Channel 23 In – 8
Channel 24 In – 9
Ground – 18
Guide Pin
OUTPUT
12 – NC
11 – CS+
10 – SCLK+
9 – MOSI+
8 – MISO+
Guide Pin
19 – Ground
20 – Channel 1 In
21 – Channel 2 In
22 – Channel 3 In
23 – Channel 4 In
24 – Channel 5 In
25 – Channel 6 In
26 – Channel 7 In
27 – Channel 8 In
28 – Channel 9 In
36 – Reference
Guide Pin
INPUT
Ground – 7
NC – 6
–CS – 5
–SCLK – 7
–MOSI – 3
–MISO – 2
+V – 1
Catalog name:
Item #:
Input Pin 36: Reference or Ground
This input can be used as a dedicated reference electrode or ground, depending on how it is set in OmniPlex Server.
the reference option in OmniPlex Server to “True Reference” to use this input as a reference. It is typically connected
Set
to a de-insulated electrode and used as a local reference for spike channels.
Set the reference option in OmniPlex Server to “grounded reference” to connect this input to ground. This is typically used
as a distant reference for field potentials, and also for spike channels.
Input Pin 1, 18 and 19: Ground
www.plexon.com
Page 49
19 – Reference #2
17 – Channel 1 In
15 – Channel 3 In
13 – Channel 5 In
11 – Channel 7 In
9 – Reference #1
7 – Channel 15 In
5 – Channel 13 In
3 – Channel 11 In
1 – Channel 9 In
25 – Ground
23 – (-V)
15 – Channel 9 In
13 – Unused
11 – Ground
9 – (-V)
7 – Channel 7 In
5 – Channel 5 In
3 – Channel 3 In
1 – Channel 1 In
Channel 4 In – 16
Channel 6 In – 14
Channel 8 In – 12
Ground – 10
Channel 16 In – 8
Channel 14 In – 6
Channel 12 In – 4
Channel 10 In – 2
Reference #2 – 26
(+V) – 24
Unused – 14
Reference #1 – 12
(+V) – 10
Channel 8 In – 8
Channel 6 In – 6
Channel 4 In – 4
Channel 2 In – 2
Reference #1
Ground – 20
Reference #2
Channel 2 In – 18
Ground – 20
N/C – 26
(+V) – 24
Unused – 14
Reference #1 – 12
(+V) – 10
Channel 8 In – 8
Channel 6 In – 6
Channel 4 In – 4
Channel 2 In – 2
Channel 10 In – 2
Channel 12 In – 4
Channel 14 In – 6
Channel 16 In – 8
Ground – 10
Channel 8 In – 12
Channel 6 In – 14
Channel 4 In – 16
Channel 2 In – 18
25 – Ground
23 – (-V)
15 – Channel 9 In
13 – Unused
11 – Ground
9 – (-V)
7 – Channel 7 In
5 – Channel 5 In
3 – Channel 3 In
1 – Channel 1 In
1 – Channel 9 In
3 – Channel 11 In
5 – Channel 13 In
7 – Channel 15 In
9 – Reference #1
11 – Channel 7 In
13 – Channel 5 In
15 – Channel 3 In
17 – Channel 1 In
19 – N/C
PRA Headstage Preamplifier Board
PRA cards accept one differential input per
16 channels in addition to having softwarecontrolled referencing. In order to choose
which reference is used as the differential
input, a removable input adaptor board
(noseboard) is used. The three types of
noseboards are the CA-11, CA-12 and
CA-13.
CA-11 Noseboard
The CA-11 input adaptor board selects the Reference #1 signal from the headset for differential subtraction in the
preamplifier. This signal is connected to pin 12 of the noseboard. All 16 channels are measured against Reference #1. CA-12 Noseboard
The CA-12 noseboard allows the user to select Reference #1 (pin 12) or Reference #2 (pin 26) by setting a jumper.
The reference selection made on the CA-12 board only affects the default reference that is used by the preamplifier in
the absence of any programmable configuration. The programmable reference selection applied to the preamplifier
supersedes this default. All 16 channels are measured against the selected reference.
www.plexon.com
Page 50
19 – Reference #2
17 – Channel 1 In
15 – Channel 3 In
13 – Channel 5 In
11 – Channel 7 In
9 – Reference #1
7 – Channel 15 In
5 – Channel 13 In
3 – Channel 11 In
1 – Channel 9 In
25 – Ground
23 – (-V)
15 – Channel 9 In
13 – Unused
11 – Ground
9 – (-V)
7 – Channel 7 In
5 – Channel 5 In
3 – Channel 3 In
1 – Channel 1 In
Ground – 20
Channel 2 In – 18
Channel 4 In – 16
Channel 6 In – 14
Channel 8 In – 12
Ground – 10
Channel 16 In – 8
Channel 14 In – 6
Channel 12 In – 4
Channel 10 In – 2
Reference #2 – 26
(+V) – 24
Unused – 14
Reference #1 – 12
(+V) – 10
Channel 8 In – 8
Channel 6 In – 6
Channel 4 In – 4
Channel 2 In – 2
13 – Channel 5 In
11 – Channel 7 In
9 – Reference #1
7 – Channel 15 In
5 – Channel 13 In
3 – Channel 11 In
1 – Channel 9 In
Channel 8 In – 12
Ground – 10
Channel 16 In – 8
Channel 14 In – 6
Channel 12 In – 4
Channel 10 In – 2
25 – Ground
23 – (-V)
15 – Channel 9 In
13 – Unused
11 – Ground
9 – (-V)
7 – Channel 7 In
5 – Channel 5 In
3 – Channel 3 In
1– Channel 1 In
15 – Channel 3 In
Channel 6 In – 14
Reference #2 – 26
(+V) – 24
Unused – 14
Reference #1 – 12
(+V) – 10
Channel 8 In – 8
Channel 6 In – 6
Channel 4 In – 4
Channel 2 In – 2
17 – Channel 1 In
Channel 4 In – 16
Reference #1
19 – Reference #2
Ground
Ground – 20
Reference #2
Channel 2 In – 18
CA-13 Noseboard
The jumper configuration for the CA-13 is horizontal which allows it to be configured when the PRA-E boards are
stacked. It functions the same as the CA-12, with all 16 channels being measured against the selected reference. PRA2 Headstage Preamplifier Board
PRA-F boards accept two
differential inputs per 16 recording
channels. The PRA-F may be
configured to use either of these
differential inputs for all 16
recording channels, or it may be
configured to use one differential
input for one group of eight
channels and the other differential
input for the other group of eight
channels. This feature may be
especially useful for comparing the
relative noise performance of two different preamplifier configurations. Additionally, you may configure the PRA-F to
ignore both the reference signal and the buffered ground signal from the headset and to use the local ground of the
preamplifier instead. The jumpers are either located inside the preamplifier box or outside the preamplifier box. If the
jumpers are located inside the preamplifier box and you decide to change the reference configuration, you will need
to un-stack the preamplifier cards. Be sure to make a note of the order in which the preamplifier boards are stacked
before disassembling them. They should be re-stacked in the same order after the jumpers have been reconfigured.
PRA2 Board Input
www.plexon.com
Page 51
PRA2 Jumpers
Jumpers may be located either inside the amplifier board or outside near the input of the preamplifier board. The
PRA-E image below illustrates the jumpers inside the preamplifier board, while the PRA-F or PRA-G jumpers shows
the jumpers outside of the preamplifier board.
PRA-E Jumpers
Channels 1-8
Use Reference #1
Use Reference #2
Use ”PBX Ground”
Channels 9-16
Use Reference #1
Use Reference #2
PRA-F or PRA-G Jumpers
Channels 1-8
Use Reference #1
Use Reference #2
Channels 9-16
Use Reference #1
Use Reference #2
www.plexon.com
Page 52
19 – Reference #2
17 – Channel 1 In
15 – Channel 3 In
13 – Channel 5 In
11 – Channel 7 In
9 – Reference #1
7 – Channel 15 In
5 – Channel 13 In
3 – Channel 11 In
1 – Channel 9 In
25 – Ground
23 – (-V)
15 – Channel 9 In
13 – Unused
11 – Ground
9 – (-V)
7 – Channel 7 In
5 – Channel 5 In
3 – Channel 3 In
1 – Channel 1 In
Ground – 20
Channel 2 In – 18
Channel 4 In – 16
Channel 6 In – 14
Channel 8 In – 12
Ground – 10
Channel 16 In – 8
Channel 14 In – 6
Channel 12 In – 4
Channel 10 In – 2
Reference #2 – 26
(+V) – 24
Unused – 14
Reference #1 – 12
(+V) – 10
Channel 8 In – 8
Channel 6 In – 6
Channel 4 In – 4
Channel 2 In – 2
PRA3 Board Input
PRA-H
PRA-H boards accept two differential inputs per 16 recording channels. The PRA-H may be configured to use either
of these differential inputs for any of the 16 channels on the board. PRA3 boards use the REF3 software (not to be
confused with similar terminology for the references on the preamplifier board) to set channels on the board to use
REF1 or REF2.
All channels on a PRA-H board are set to use REF1 as the reference when the board is powered ON.
PRA-I
PRA-I boards accept two differential inputs per 16 recording channels. The PRA-I may be configured to use either of
these differential inputs for all 16 recording channels, or it may be configured to use one differential input for one
group of eight channels and the other differential input for the other group of eight channels. You may also configure
the PRA-I to ignore both the reference signal and the buffered ground signal from the headset and use the local
ground of the preamplifier instead.
www.plexon.com
Page 53
PRA3 Jumpers
The jumpers may be located near the input of the preamplifier board, so that they are accessible from the outside
when the boards are stacked. This board is called the PRA3-I and the reference can be set the same as the PRA-H.
Channels 1-8
Use Reference #1
Use Reference #2
Channels 9-16
Use Reference #1
Use Reference #2
www.plexon.com
Page 54
Biopotential Headstage Electrode Interface Board (EIB) - Single Input
The Electrode Interface Board (EIB) is for use with Plexon’s biopotential/differential headstages. The EIB facilitates
connection of electrodes to the headstage, has one electrode contact per channel and comes in two variations.
Variation #1: No pins installed
The first variation (12-03-A-18-00) provides individual 0.021” in diameter
gold plated holes for each electrode connection and three holes for the
ground connections. Electrode wires may be soldered directly to these holes,
or press fit into the EIB using large sized conical EIB pins. When using the
EIB pins, the electrode wire is threaded through the hole from one side and
the EIB pin is pressed into the hole from the other side, crimping the wire
into the hole as shown below:
EIB pin
Electrode wire
Variation #2: Pins installed
The second variation (12-03-A-18-18) comes with pins (Mill-Max 4366)
installed in each of the electrode holes and two of the ground holes.
Electrode wires may be soldered to caps (Mill-Max 0334 not included) and
the caps may be reversibly connected to the individual pins on the interface
board as shown below:
Converting grounded electrode connection holes
In both 12-03-A-18-00 and 12-03-A-18-18, all of the electrode connection holes are initially connected to ground
by means of a cuttable trace on the bottom side of the circuit board. To use a particular electrode connection, the
corresponding ground trace on the bottom side of the circuit board must be cut. For example, to use the positive
input for channel 1, the trace marked with the red ‘X’ should be cut in the picture below. Unused electrode inputs
should remain grounded.
Negative Inputs
Positive Inputs
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Cuttable Traces
Page 55
Biopotential Headstage Electrode Interface Board (EIB) - Dual Input
The Electrode Interface Board (EIB) is for use with Plexon’s biopotential/differential headstages. The EIB facilitates
connection of electrodes to the headstage, has two electrode contacts per channel and comes in two variations.
Variation #1: No pins installed
The first variation (12-03-A-21-00) provides individual 0.022” in diameter
gold plated holes for each electrode connection and five holes for the
ground connections. Electrode wires may be soldered directly to these
holes, or press fit into the EIB using large sized conical EIB pins. When using
the EIB pins, the electrode wire is threaded through the hole from one side
and the EIB pin is pressed into the hole from the other side, crimping the
wire into the hole as shown below:
EIB pin
Electrode wire
Variation #2: Pins installed
The second variation (12-03-A-21-36) comes with pins (Mill-Max 4366)
installed in each of the electrode holes and four of the ground holes.
Electrode wires may be soldered to caps (Mill-Max 0334 not included) and
the caps may be reversibly connected to the individual pins on the interface
board as shown below:
Converting grounded electrode connection holes
In both 12-03-A-21-00 and 12-03-A-21-36, all of the electrode connection holes are initially connected to ground
by means of a cuttable trace on the bottom side of the circuit board. To use a particular electrode connection, the
corresponding ground trace on the bottom side of the circuit board must be cut. For example, to use the positive
input for channel 1, the trace marked with the red ‘X’ should be cut in the picture below. Unused electrode inputs
should remain grounded.
Negative Inputs
Positive Inputs
Cuttable Traces
www.plexon.com
Page 56
Using G20 Headstages to Record Field Potentials
Plexon local field potential preamplifier boards incorporate per-channel band-pass filters for removing spectral
content outside the frequency range of interest. Low-cut filters are used to recover signal recording capability after
movement artifacts or other voltage transients, whereas high-cut filters are used to remove spike signals from the
LFP band and allow digitization of the LFP signals at a reasonable sampling rate (typically 1kHz) without aliasing. In
addition, Plexon’s gain 20x VLSI headstages contain low-cut filters (< 1Hz cutoff), to remove any DC potential that
may accumulate at the electrode, metal-brain interface.
These filters, like most filters, inherently produce frequency-dependent phase shifts in the signal. The phase shifts are
equivalent to time delays (known as group delays) which likewise, vary with frequency. A way to avoid this problem is
ensure the filter cutoff values (the -3dB values) are a factor of ten or more away from the field potential frequency of
interest. In the case of the common 0.7-300 Hz band pass LFP filter, for instance, the filter will cause signals between
7-30 Hz to be delayed by less than 16 degrees for any frequency component. Outside this frequency range, the
delays can be as large as 180 degrees of the period (i.e. 50% of the period). However, it is possible to post-process
field potential recordings using the Plexon FPAlign utility so that the timing delays are greatly reduced. After running
your .PLX file through the FPAlign utility, the timing errors in the frequency components of the LFP signal (all the way
up to the -3 dB cutoff frequencies) will be greatly reduced, and can then be used in subsequent analyses that are
sensitive to LFP phase information.
NOTE: FPAlign can only work correctly when the preamplifier and headstage filtering that was used at the
time of recording is known.
The FPAlign utility may be downloaded from the Plexon website at www.plexon.com/software-downloads.
The download includes a document that provides the LFP-band phase delays as a function of frequency for some standard Plexon preamplifier and headstage filters, and the residual delays as a function of frequency
after FPAlign processing.
Headstage and Conversion Options
Light Emitting Diode (LED)
Most Plexon analog and digital headstages can be equipped with mounted LEDs for use
in behavioral analysis research using Plexon’s CinePlex® Behavioral Research System with
CinePlex Tracking. Your choice of red, blue or green LEDs is available. The headstage
pictured to the right is a 0.025” pitch, 32 channel headstage with two LEDs for highimpedance electrodes (HST/32o25-36P-2LED).
The LED Power pictures shown below are for the HST/16V-G20-2LED and the
HST/32V-G20-2LED headstages.
www.plexon.com
Page 57
Latching
More active animals may require an especially secure connection between the
mating connector and the headstage’s insertion pins. Plexon’s 0.050” pitch, 8
channel headstages are available with a special clipping (latching) mechanism that
delivers this additional security. The headstage pictured to the right is a 0.050”
pitch, 8 channel headstage for high-impedance electrodes with the latching
option (HST/8o50-G1-GR-L).
Ground Reference to True Reference Conversion
To configure a Ground Reference headstage to True Reference, there is the option to cut the trace connecting input
pins 9 and 10 and/or pins 19 and 20 on some headstage models. Doing so creates a dedicated reference input(s).
Cutting trace connecting pins 9 and 10 creates Reference #1, while cutting trace connecting pins 19 and 20 creates
Reference #2.
There are other headstage configurations not pictured here that may have a different trace layout. If you have any
questions concerning which trace to cut in order to configure a given headstage from Ground Reference to True
Reference, please contact Plexon support at [email protected] or visit our websites technical support page at
www.plexon.com/plexon-technical-support.
Below are two examples of where to cut to disconnect pin 9 from Ground (GND) in both a G1 and HST/8o50-Gx-TR
headstage:
HST/8o50-G1-TR
Cut here to disconnect
pin 9 from GND
HST/8o50-G20-TR
Cut here to disconnect
pin 9 from GND
www.plexon.com
Page 58
DigiAmp™ Connector Pinout, Ground and Reference Information
This section describes the connections and pinouts on the front panel of the DigiAmp™ Amplifier.
Sig Com—Signal common is the local zero-voltage reference point of the DigiAmp unit.
Earth—Earth is a direct connection to Earth ground. In the United States, Earth ground connects to the third prong
on the wall outlet, which is eventually tied to hard earth at the power service entrance to the building.
NOTE: Ambient noise in buildings and noise radiated by electronic equipment are very common, and
they can interfere with the signals in your experiment. To help reduce noise problems, you can connect
the green ground wire from either Sig Com or Earth to metal object(s) near the animal being studied,
such as headposts or apparatus framing or plates. It is best to try connecting to Sig Com and observing
the noise reduction effect, then connecting instead to Earth and observing the effect, then comparing
the results. Use the connection that gives the best results (best noise reduction).
Isolated/Grounded switch—The switch allows you to directly connect Sig Com to Earth ground or not. If Sig Com is
not directly connected to Earth ground, the DigiAmp unit is isolated from Earth ground.
HST PWR—This switch turns the power supply to the headstages on or off.
The DigiAmp Amplifier can be supplied with up to eight 42-pin headstage connectors. Each connector has pins for 32
channels, four pins for reference signals (RefA and RefB), two pins for power (+V and –V) and two pins for DigiAmp
signal common/ground (Sig Com/Ground). Two pins are reserved (not connected).
NOTE: The PBX boxes (used either alone or with a MAP or Recorder system) use “REF 1” or “REF 2”
to indicate input pins 12 or 26 (respectively). The OmniPlex Amps (OPX-A, OPX-D, and DHP) use
“REF A” and “REF B” to refer to those same reference lines. Any time you see “REF 1” or “REF 2” in
the headstage technical guide, you can treat them as “REF A” and “REF B” when using an
OmniPlex system.
www.plexon.com
Page 59
NC – 42
(-V) – 40
Sig Com/Ground – 38
RefB (Ch 17-32) – 36
Channel 32 – 34
Channel 30 – 32
Channel 28 – 30
Channel 26 – 28
Channel 24 – 26
Channel 22 – 24
Channel 20 – 22
Channel 18 – 20
RefB (Ch 1-16) – 18
Channel 16 – 16
Channel 14 – 14
Channel 12 – 12
Channel 10 – 10
Channel 8 – 8
Channel 6 – 6
Channel 4 – 4
Channel 2 – 2
41 – NC
39 – Sig Com/Ground
37 – (+V)
35 – RefA (Ch 17-32)
33 – Channel 31
31 – Channel 29
29 – Channel 27
27 – Channel 25
25 – Channel 23
23 – Channel 21
21 – Channel 19
19 – Channel 17
17 – RefA (Ch 1-16)
15 – Channel 15
13 – Channel 13
11 – Channel 11
9 – Channel 9
7 – Channel 7
5 – Channel 5
3 – Channel 3
1 – Channel 1
The diagram below shows the pinouts for Channels 1 to 32, which are on the first connector. Notice that there is one
RefA pin for the first set of 16 channels (RefA Ch 1-16) and a different RefA pin for the second set of 16 channels
(RefA Ch 17-32). Similarly, there is a RefB (Ch 1-16) and a RefB (Ch 17-32).
The functions of the pins are as follows:
RefA and RefB—Reference signal input provided by the headstage to the DigiAmp unit. You can select RefA or RefB
through the DigiAmp Device Settings dialog box. (See OmniPlex® User Guide v1 “Appendix E: DigiAmp Analog Highpass
Filter and Referencing” on page A-26). The resultant signal for a particular channel is determined by subtracting the RefA
or RefB signal from the signal measured at the pin assigned to that channel. For example, the resultant signal for Channel
21 is the signal on pin 23 (Channel 21 is assigned to pin 23) minus the signal on pin 35 (if RefA is selected) or pin 36 (if RefB
is selected).
+V and –V—Power provided by the DigiAmp unit to headstages, +3.0V and 3.0V with respect to DigiAmp Sig Com/
Ground.
Sig Com/Ground—Signal common and ground. Pins labeled “Ground” on headstage cables and headstages should
be connected to signal common. Signal common in the DigiAmp Amplifier can be either isolated from Earth ground or
connected to Earth ground. (See the OmniPlex User Guide v1 for information about the Isolated/Grounded switch on page
A-51.)
NC—No connection (not used).
There are two 42-pin connectors placed side by side on a board, and each board supports 64 channels. The DigiAmp
can have up to four boards for a total of 256 channels.
The pinouts for all 256 channels on the eight 42-pin connectors follow the pattern shown in this diagram.
Channels 32-1
Channels 64-3
Channels 96-95 Channels 128-197
www.plexon.com
Page 60
MiniDigi™ Connector Pinout, Ground and Reference Information
This section describes the connections and pinouts on the front panel of the MiniDigiAmp™ Amplifier.
Sig Com—Signal common is the local zero-voltage reference point of the MiniDigi unit.
Earth—Earth is a direct connection to Earth ground. In the United States, Earth ground connects to the third prong
on the wall outlet, which is eventually tied to hard earth at the power service entrance to the building.
NOTE: Ambient noise in buildings and noise radiated by electronic equipment are very common, and
they can interfere with the signals in your experiment. To help reduce noise problems, you can connect
the green ground wire from either Sig Com or Earth to metal object(s) near the animal being studied,
such as headposts or apparatus framing or plates. It is best to try connecting to Sig Com and observing
the noise reduction effect, then connecting instead to Earth and observing the effect, then comparing
the results. Use the connection that gives the best results (best noise reduction).
Isolated/Grounded switch—The switch allows you to directly connect Sig Com to Earth ground or not. If Sig Com is
not directly connected to Earth ground, the DigiAmp unit is isolated from Earth ground.
HST PWR—This switch turns the power supply to the headstages on or off.
The MiniDigi Amplifier can be supplied with up to four 26-pin headstage connectors. Each connector has pins for 16
channels, two pins for reference signals (RefA and RefB), four pins for power (+V and –V) and two pins for MiniDigi
signal common/ground (Sig Com/Ground). Two pins are reserved (not connected).
NOTE: The PBX boxes (used either alone or with a MAP or Recorder system) use “REF 1” or “REF 2”
to indicate input pins 12 or 26 (respectively). The OmniPlex Amps (OPX-A, OPX-D, and DHP) use
“REF A” and “REF B” to refer to those same reference lines. Any time you see “REF 1” or “REF 2” in
the headstage technical guide, you can treat them as “REF A” and “REF B” when using an
www.plexon.com
Page 61
RefB (Ch 1-16) – 26
(+V) – 24
Channel 16 – 22
Channel 14 – 20
Channel 12 – 18
Channel 10 – 16
NC – 14
RefA (Ch 1-16) – 12
(+V) – 10
Channel 8 – 8
Channel 6 – 6
Channel 4 – 4
Channel 2 – 2
23 – (-V)
21 – Channel 15
19 – Channel 13
17 – Channel 11
15 – Channel 9
13 – NC
9 – (-V)
7 – Channel 7
5 – Channel 5
3 – Channel 3
1 – Channel 1
The diagram below shows the pinouts for Channels 1 to 16, which are on the first connector.
The functions of the pins are as follows:
RefA and RefB—Reference signal input provided by the headstage to the MiniDigi unit. You can select RefA or RefB
through the DigiAmp Device Settings dialog box. (See OmniPlex User Guide v1 “Appendix E: DigiAmp Analog Highpass
Filter and Referencing” on page A-26). The resultant signal for a particular channel is determined by subtracting the RefA
or RefB signal from the signal measured at the pin assigned to that channel. For example, the resultant signal for Channel
14 is the signal on pin 20 (Channel 14 is assigned to pin 20) minus the signal on pin 12 (if RefA is selected) or pin 26 (if RefB
is selected).
+V and –V—Power provided by the MiniDigi unit to headstages, +3.0V and 3.0V with respect to MiniDigi Sig Com/
Ground.
Sig Com/Ground—Signal common and ground. Pins labeled “Ground” on headstage cables and headstages should
be connected to signal common. Signal common in the MiniDigi Amplifier can be either isolated from Earth ground or
connected to Earth ground. (See the OmniPlex User Guide v1 for information about the Isolated/Grounded switch on page
A-54.)
NC—No connection (not used).
There is one 26-pin connector on a board, and each board supports 16 channels. The MiniDigi unit can have up to
four boards for a total of 64 channels.
The pinouts for all 64 channels on the four 26-pin connectors follow the pattern shown in this diagram.
Channels 16-1
Channels 64-49 www.plexon.com
Page 62
About Plexon Inc
Plexon is a pioneer and leading innovator of custom, high-performance data acquisition, behavior and analysis solutions specifically designed
for scientific research. We collaborate with and supply thousands of customers including the most prestigious neuroscience laboratories
around the globe driving new frontiers in areas including basic science, brain-machine interfaces (BMI), neurodegenerative diseases, addictive
behaviors and neuroprosthetics. Plexon offers integrated solutions for in vivo neurophysiology, optogenetics, and behavioral research – backed
by its industry-leading commitment to quality and customer support. For more information, please visit www.plexon.com.
Sales Support
For Sales Support, email [email protected] or call +1 (214) 369-4957.
Technical Support
If after reviewing this document, you would still like to access Plexon’s Technical Support, we are available via several communication channels.
You are invited to reach us through email, on the phone, or even over Skype utilizing instant messaging, voice, and/or video as follows:
EMAIL
PHONE
INSTANT MESSAGING, VOICE OR VIDEO VIA SKYPE
[email protected]
8:30 a.m. to 5:00 p.m. Central Time
+1 (214) 369-4957
8:30 a.m. to 5:00 p.m. Central Time
Skype name: plexonsupport
Skype is a free service. For more information on Skype or to
download the application, go to www.skype.com.
PLEXON®, the five-line symbol, CereStage™, CineCorder™, CineLAB™, CineLyzer™, CinePartner™, CinePlex®, CineTracker™, CineTyper™, DigiAmp™, MiniDigi™,
Offline Sorter™, OmniPlex®, PL2™, PlexBright®, PlexDrive™, PlexStim™, Radiant™ and RapidGrid™ are registered and unregistered trademarks of Plexon Inc,
Dallas, Texas, USA. ©2015 Plexon Inc. All rights reserved. Other product and company names mentioned are trademarks of their respective owners.
HSTTN0001h
www.plexon.com
Page 63

Headstage Technical Guide

Transcription

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