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June, 3rd, 2010 Soil fertility and crop management A. Oswald, J. Sanchez, G. Quispe, M. Rojas, P. Calvo, J. Caycho Introduction Integración socioeconómica MIE Suelo Semillas MIP Integración de componentes Introduction Goals: Contribute to the development of integrative research approaches in division 4 and CIP; Establish soil fertility and cropping systems management as a component of ICM in division 4 ; Contribute to an intensification of collaborative activities among divisions and partner programs; Support NARES in capacity building and soil fertility research; Extent activities to other CIP-regions; Integrative Research Contribute to the development of integrative research approaches in division 4; - time consuming task; - depending on the effort and attitude of scientists involved; - requires leadership and commitment; - eventually removed from the priority activities; Collaborative activities Contribute to an intensification of collaborative activities among divisions and partner programs; - collaborative activities division 3 and 5; - Papa Andina, ALTAGRO, Urban Harvest, CONDESAN; With other institutions – NARS, NGOs etc.; Support capacity strengthening Support NARES in capacity building and soil fertility research; - INIA, INIAP - PROINPA; - CEDEPAS, YANAPAI conclusions: need for basic training and standardization - design, implementation, evaluation and statistical analysis; - time series - locations participatory approaches; development of training approaches; feed-back – robustness of methods and technologies – scaling out Soil fertility & crop management research Establish soil fertility and cropping systems management as a component of ICM in division 4; Soil fertility and fertilizer use ¾ organic amendments ¾ manure, compost, biol ¾ inorganic amendments ¾ fertilizer use, types, efficiency Crop management ¾ minimum tillage and use of mulch ¾ in the Andes ¾ in China ¾ traditional potato production technologies ¾ development of a concept of sustainable agricultural systems and evaluation of technologies Biological amendments ¾ PGPR ¾ crop production and protection ¾ seed production ¾ Mycorrhiza ¾ crop production Soil fertility and fertilizer use I. Present situation Location % of farmers appl. fertilizer N in kg ha-1 P in kg ha-1 K in kg ha-1 10.0 Aymara n=20 100 160 ± 46 209 ± 44 186 ± 53 7.5 26.6 26 ± 18* 9±7 22 ± 16 27.5 39.3 Choppcas n=25 22 2 3 12.5 21.3 97 195 ± 110 144 ± 61 117 ± 72 4 15.0 2.7 Marcavalle n=35 5 2.5 -- Aramachay n=33 100 133 ± 77 156 ± 113 107 ± 41 Duration of fallow period in years Altiplano Aramachay 0 35.0 1 Altiplano n=214 21 55 ± 45* 0 manure in kg ha-1 OM* in kg ha-1 N in kg ha-1 P in kg ha-1 K in kg ha-1 Location Average area cultivated with potato in ha Aymara n=20 1.50 ± 0.47 4070 ± 1132 1741 ± 609 76 ± 23 44 ± 12 107 ± 30 Choppcas n=25 0.74 ± 0.58 3637 ± 3110 1642 ± 1525 58 ± 52 35 ± 32 59 ± 49 Marcavalle n=35 0.45 ± 0.33 4240 ± 2370 2014 ± 1012 85 ± 41 42 ± 34 47 ± 45 Aramachay n=50 0.46 ± 0.25 10076 ± 6611 3869 ± 2539 147 ± 83 118 ± 67 374 ± 212 Altiplano n=214 0.55 ± 0.55 5365 ± 2455 2441 ± 1117 86 ± 39 45 ± 21 96 ± 44 0 Soil fertility management Relation of OM applications and tuber yield in Choppcas and the Altiplano Organic matter in kg/ha O rg a n ic m atter in kg /h a Relation of OM applications and tuber yield in Aramachay and Aymara 6,000 4,000 2,000 0 0 10000 20000 30000 40000 6,000 4,000 2,000 0 50000 0 10000 Tuber yield in kg/ha 200 100 Relation of N and OM applications in Aramachay and Aymara 300 N fertilizer in kg/ha N fertilizer in kg/ha y = -0.0027x + 209.32 2 R = 0.287* 200 100 y = 0.0224x + 75.99 2 R = 0.316* 0 0 10000 20000 30000 Tuber yield in kg/ha 30000 Tuber yield in kg/ha Relation of N applications and tuber yield in Aramachay and Aymara 300 20000 40000 50000 0 0 1,000 2,000 3,000 4,000 Organic matter in kg/ha 5,000 6,000 Input Aymara Aramachay Output - 21 t/ha Input Output Choppcas - 27 t/ha Input Output Altiplano - 13 t/ha Input - 11 t/ha Output 0 Fertilizer N 100 200 Manure N (50%) 300 Soil N 400 500 Potato N N input – output relationship in farmers‘ fields of communities in the Central Andes Aymara Input Output Aramachay Input Output Choppcas Input Output Altiplano Input Output 0 100 Fertilizer N Potato N 200 300 Manure N Barley N 400 500 600 Soil N Oats N N input – output relationship in farmers‘ fields of communities in the Central Andes Soil fertility and fertilizer use I. Organic amendments - home made abundant availability produced based on robust, easy processes - nutrient source increase nutrient use efficiency improve plant recovery after stress events Organic amendments – Biol / manure tea Ingredientes Agua (l) Estierco (kg) 2 Melaza (kg) Leguminosa (kg) Leche (l) Ceniza (kg) Levadura (kg) Nitrógeno (kg) Fósforo (kg) Capacidad en l 1 Biodigestores (tratamientos) 1 2 31 90 90 90 15+5 0+20 15+5 2.5 1 1.5 0.5 0.25 4 90 15+5 2.5 5 90 15+5 2.5 6 90 15+5 2.5 1.5 0.5 0.25 7 90 0+20 2.5 1 1.5 0.5 0.25 8 90 0+20 5 1 9 90 0+20 5 1 0.25 1 0.25 0.9 0.9 120 120 120 120 120 0.9 120 120 120 = tratamientos 3 y 12 tienen los mismos ingredientes, pero tratamiento 12 fue removido 2 veces al día; 120 10 90 0+20 5 1 0.9 0.9 120 0.5 121 90 15+5 2.5 1 1.5 0.5 0.25 120 120 11 90 15+15 5 2 3 Organic amendments – Biol / manure tea pH 18 dias 36 dias 58 dias 72 dias 92 dias 107 dias 120 dias 8.00 6.00 4.00 2.00 0.00 1 2 3 4 5 6 7 8 Tratamientos 9 10 11 Peso fresco promedio (g/maceta) Básicos 8.2 Ácidos 12.0 Fertilizante foliar (20-20-20) 14.3 Sin aplicación 9.8 12 Figura 1: Lectura de pH de los Bioles (7 noviembre 2008 - 9 marzo 2009) Basicos Grupos Acidos Basicos Acidos 6000.00 120.00 5000.00 100.00 mg/L mg/L 4000.00 3000.00 2000.00 80.00 60.00 40.00 20.00 1000.00 0.00 0.00 N P K Ca Macroelementos Mg Na Cu Zn Mn Microelementos Fe B Organic amendments – compost 50 g/planta 40 30 20 10 0 Control Compost-llama Compost-cow Compost-chicken 10 g/maceta 20g/maceta Field trial 15 12 9 6 3 ur e 12 012 012 Ll 0 am a 1: 1 f Ll am a 2: 1 f Ll am a 2: 1 Co w 1: 1 f Co w 2: 1 f Co w 2: Ch 1 ic ke n 1: 1 Ch f ic ke n 2: 1 Ch f ic ke n 2: 1 NP K t/h a m an Co n tr ol 0 10 Compost: -C/N – 25:1 -pathogen free -low or no ammonium -activates soil life -slow release of nutrients -improves soil structure – aeration, moisture holding capacity, CEC Pot trial Soil fertility and fertilizer use Rendimiento comercial y total en t/ha 30 t/ha 8 25 6 20 4 15 10 2 5 0 0 Control Marcavalle Ñuñunhuayo 8 t/ha 16 t/ha 24 t/ha 80 N Tingo 160N 8t/ha + 8t/ha + 80 N 160 N Soil fertility and fertilizer use 20 15 10 5 0 Huamantanga Camotillo Peruanita Yana Huayro 16t manure 24t manure NPK 80-100-100 NPK 160-100-100 Crop management I. Minimum tillage I. Andes Barbecho Chaqmeo Chiwa Crop management I. Minimum tillage I. Andes 20 15 10 5 organic 3755m asl inorganic 4102m asl 4144m asl 20 15 10 5 Barbecho 3755m asl Oswald et al. 2009. The complexity of simple tillage systems. Journal of Agricultural Sciences, 147, 399-410 Chacmeo 4102m asl Chiwa 4144m asl II. Minimum tillage - China Potentials and opportunities ¾ ¾ ¾ Jan new short duration rice varieties increase winter fallow period; temperatures adequate for potato production; winter potato intensifies land-use-system; Feb Mar Apr May Jun Jul Aug Sep winter potato Oct Nov Dec winter potato early rice late rice spring potato summer rice rapeseed, vetch barley, wheat rapeseed, vetch barley, Oswald et al. 2009. Minimum tillage systems with potato in winter cropping regions of subtropical China. Tropentag – Biophysical and socio-economic frame conditions for sustainable management of natural resources. 06-08.10.2009, Hamburg, Germany Winter potato cropping system ¾ ¾ ¾ farmers’ innovation; often as minimum tillage system on heavy rice soils; several types according to climatic or soil conditions; Soil fertility and fertilizer use Biological amendments - Plant Growth Promoting Bacteria - PGPR Mycorrhiza - PGPR - Laboratory tests Greenhouse experiments Field trials Pot trials Canchan Yungay Control control control B1 C2 30 30 20 20 10 10 0 C1 C2 B1 B2 B3 B4 B5 Tuber Plant Variedad Canchan B6 B7 B8 0 C1 C2 C2 B9 B9 B10 B11 B12 B13 B14 B15 B16 Tuber Plant Oswald et al., 2010 Evaluating soil rhizobacteria for their ability to enhance plant growth and tuber yield of potato. Annals of Applied Biology (in press). Crop diversity 120 10 semanas 11 semanas 100 80 g/plant 60 40 Control 8 semanas Azo16M2 Act16M2 11 semanas 20 Control 0 Acelga Bac17M9 Rabanito Control Bac 22 Espinaca Act 16M2 Maiz Azo 16M2 - all crops showed increments in fresh and dry matter - plant weight increased between 40% - 140% Bac17M9 Azo16M2 Act16M2 PGPR use in different production systems I. Aeroponics Variety Peruanita with PGPR 1.00E+10 Variety Peruanita without PGPR Planting 60 days 1.00E+08 60 days 30 days 1.00E+06 cfu 60 days ¾ ¾ 1.00E+04 ¾ 1.00E+02 1.00E+00 Inocul. Water Water Total bacteria Water Canchan Inocul. Bacillus Azotobacter Noninoc. Tubers production from inoculated plants better growth of inoculated plants; the growth period of inoculated plants was extended by up to 30 days; adequate bacteria populations could be maintained in the irrigation water and on potato roots; Aeroponics (20) 100 500 (6) 400 80 (78) (63) (83) 60 (-14) (42) (38) 300 (4) 40 20 (-12) (82) (72) (-1) (-5) 200 (126) (133) 100 (665) (315) 0 0 Des Revo Cruza Conde Unica Capiro Perri Yun Can Des Number of tubers/plant Revo Cruza Conde Unica Capiro Perri Yun Can Tuber yield in g/plant 8 ¾ 6 4 ¾ 2 0 Des Revo Cruza Conde Unica Capiro Mean tuber weight in g with bacteria without bacteria Perri Yun Can inoculated plants produce more tubers and often greater tuber weights; tuber size is similar or greater with non-inoculated plants Farmers’ greenhouse Yields in kg/m2 of horticultural crops in 3 greenhouses in Puno, Peru Location Palermo Yacari 1 control A1-15/06 Chard 3.28 + 60% 4.69 + 41% 4.88 + 60% Spinach 3.63 + 14% 3.44 + 2% 3.88 + 29% Beetroot 4.38 + 3% 4.69 + 3% 7.81 - 20% Radish -- -- 4.38 - 13% 5.31 - 29% Pumkin -- -- 1.62 + 42% -- -- Bacteria control B1-22/06 Yacari 2 control A3-30/06 Farmers’ fields – horticultural crops 60 45 30 15 Yield increase of coriander or beetroot inoculated with PGPR strains over control without bacteria at farmers’ fields in Huachipa, Lima. 0 -15 -30 A1-22/06 A1-17/06 B1-22/06 B1-27/06 A3-25/06 A3-27/06 Coriander Beetroot Field trials with potato ¾ Field evaluation: cultivars, agro-ecologíes, crops ¾ Trials implemented: Lima (0 m asl), Huancayo (3,300m asl); Aymara (3,900m asl), Puno (3,900m asl) ¾ Varieties: Única, Revolución, Yungay, Amarillis, Ccompis, Amarillo del Centro, Andina, Queccorani ¾ Crops: potato and maize ¾ 2007 – 2009 14 trials with and potato and 1 trial with maize ¾ 64 strains used: 21 Bacillus, 8 Azospirillum,13 Azotobacter, 8 Pseudomonas, 14 Actinomycetes ¾ 13 positive strains: 4 Bacillus 1 Azospirillum 4 Azotobacter 3 Actinomycetes 1 Pseudomonas 19% 13% 31% 21% 13% Results of fields trials 2006-2009 Strain A1-30/06 Actinomycetes A1-30/08 A1-45/08 A2-18/08 Azospirillum A3-15/06 Azotobacter A3-16/08 A3-39/08 B1-04/06 Bacillus B1-05/06 B1-21/06 B1-22/06 B1-29/06 B1-35/06 P1-20/08 Pseudomonas B1-35/06+P1-20/08 Number of field trials Positive yield response 10 2 1 1 5 3 3 2 2 8 5 3 4 2 1 3 1 1 1 2 2 2 1 1 1 2 1 1 1 1 % increase 11-30 19 16 10 10 9-13 10-23 14 8 38 11-17 28 18 16 9 Pot trial using bacterial strains to inoculate potato plants growing in substrate with different pH dry tuber weight in g 12 9 6 3 0 4.5 5.6 6.8 7.8 pH P1-20/08 B1-35/06 A2-20/08 A3-19/08 Control Peso seco de tuberculos in g de plantas de papa inoculadas con rhizobacterias en substratos con diferentes texturas 20 tuberculos in g/plant 15 10 5 Arena Musgo Tierra 100 0 0 80 20 0 60 40 0 40 60 0 0 10 90 0 25 75 0 50 50 20 40 40 0 P1-20/08 B1-35/06 A2-19/08 A3-19/08 A1-19/08 Control 200 Incrementos en % del 150 peso seco de tuberculos de plantas de papa inoculadas con % 100 rhizobacterias en substratos con diferentes 50 texturas 0 P1-20/08 B1-35/06 A2-19/08 A3-19/08 A1-19/08 Results of fields trials 4.2 t/ha Control 17.2 t/ha P1-08/08 B1-21/06 A2-01/08 A3-39/08 A3-16/08 A1-30/08 A1-30/06 -20 -10 0 10 20 30 % increase/reduction over control Salcedo Tahuaco 40 Future activities I. Fertilizer use efficiency I. II. II. Minimum tillage I. II. III. Use in pre-basic seed production; Use in marginal environments and low-input systems; Improve the quality and efficiency of organic fertilizers; Nutrient management I. II. V. Developing minimum tillage systems for tuber crops; Developing MT options for marginal environments; Biological amendments I. II. III. IV. Improving the efficiency of organic inputs for low-input systems; Improving the efficiency of inorganic fertilizers for high input systems; Farm nutrient inventories; Capacity building in nutrient management – NARS and farmers Genetic improvement I. II. Screening for fertilizer use efficiency; and/or nutrient appropiation capacity; gracias