Zeiss AxioImager.Z1/ ApoTome Microscope

CB&IACore
The University of Ottawa
CB&IACore Facility
Facility
Zeiss AxioImager.Z1/
ApoTome
Microscope
Objectives
Zeiss Axio Imager Z1 Microscope Objectives
Room: 3123
1
2
3
EC Plan-Neufluar 20x
EC Plan-Neofluar 10x
EC Plan Neofluar 5x
0.5 Ph2
0.3 M27
0.16
Magnification
20x
10x
5x
Numerical Aperture
0.5
0.3
0.16
2
5.2
18.5
Coverglass (mm)
0.17
0.17
0.17
Immersion
None
None
None
ICS
ICS
ICS
Flatness
****
****
****
Color Correction
****
****
****
UV Transmission
****
****
****
IR Transmission
***
***
***
DIC
-
**** (not equipped)
**** (not equipped)
High Contrast DIC
-
-
-
Ph2
-
-
440341-9904-000
420340-9901-000
440320-9902-000
4
5
6
EC Plan Neofluar 40x
Plan Apochromat 63x
EC Plan-Neofluar 100x
0.75 M27
1.4 Oil M27
1.3 Oil M27
Magnification
40x
63x
100x
Numerical Aperture
0.75
1.4
1.3
Working Distance (mm)
0.71
0.19
0.2
Coverglass (mm)
0.17
0.17
0.17
Immersion
None
Oil
Oil
ICS
ICS
ICS
Flatness
****
*****
****
Color Correction
****
*****
****
UV Transmission
****
***
****
IR Transmission
***
****
***
**** (not equipped)
DIC III
(DIC III)****
-
-
Yes
-
420360-9900-000
420780-9900-000
420490-9900-000
Working Distance (mm)
Optical System
Phase Contrast
Catalog #
Optical System
DIC
High Contrast DIC
Phase Contrast
Catalog #
ICS=Infinity Color Corrrected System
Filter Sets
Zeiss Axio Imager.Z1 Microscope Filter Sets
Room 3123
1. Transmitted Light
2. Filter Set 49 (DAPI)
Excitation:
G
365
Beam Splitter:
FT
395
Emission:
BP 445/50
3. Filter Set 47 (CFP)
Excitation:
BP
Beam Splitter:
FT
Emission:
BP
436/20
455
480/40
4. Filter Set 10 (Cy2/GFP)
Excitation:
BP 450-490
Beam Splitter: FT
510
Emission:
BP 515-565
5. Filter Set 46 (YFP)
Excitation:
BP
Beam Splitter:
FT
Emission:
BP
500/20
515
535/30
6. Filter Set 31 (Cy3.5/mcherry)
Excitation:
BP
565/30
Beam Splitter: FT
585
Emission:
BP
620/60
7. Filter Set 50 (Cy5)
Excitation:
BP
Beam Splitter:
FT
Emission:
BP
640/30
660
690/50
8. ApoTome Calibration
https://www.micro-shop.zeiss.com/?p=us&f=f
Filter Sets
https://www.micro-shop.zeiss.com/?p=us&f=f
Filter Sets
https://www.micro-shop.zeiss.com/?p=us&f=f
Filter Sets
https://www.micro-shop.zeiss.com/?p=us&f=f
Attention Users!!
Immersion OIL Rules:
 Always keep immersion oil bottle CLOSED. This prevents
water evaporation and maintains the integrity of the oil
 Keep oil bottle CLEAN. Oil should never be found along the
sides of the bottle or around the neck
 Apply only a SMALL AMOUNT of oil to your coverslip
Oil droplet
Coverslip
 Always load your sample on the microscope stage with the
STAGE LOWERED to the lowest focus position
 Always REMOVE OIL using dry Lens Paper from the objective
when changing slides. Re-apply a small amount of new oil to
the new slide
 If you are imaging a sample using an oil objective, do not
toggle between objectives. Image with that objective only!
 When you are done your session, clean objective using Dry
Lens Paper
 Do not get oil on any air objectives!
Attention Users!!
FluoArc Mercury Lamp:
The FluoArc lamp must be on for at least 30 minutes before being
turned off AND must not be turned on again within 1 hour of
being turned off.
1. When starting, before you turn on the FluoArc lamp, make sure it
has been OFF for at least 1 hour from previous use.
2. When finishing, before you turn off the FluoArc lamp, make sure it
has been ON for at least 30 minutes.
Objectives:
If you use oil on an oil-objective, please wipe it off with dry Lens
Paper after you have finished.
ApoTome:
Before toggling ApoTome power box switch off, make sure that
ApoTome slider is pulled out to click stop position 1
Your Data:
You can temporarily save your data under My Documents, your
lab-folder, your folder
You are responsible for backing-up your own data at all times. Do
not rely on this computer as storage for your data. Once you have
backed-up your files elsewhere, please delete your files from this
computer.
Attention Users!!
Log Book:
All users must log the time spent on the station as well as the time
the fluorescence lamp was turned on/off in the log book.
Guest Users:
Others and guest users can (with permission) use this microscope
on Mondays and Thursdays only.
Computer:
This computer is to be used only for image acquisition.
No other work is permitted!!
Zeiss Imaging System Help Line:
1-800-509-3905
Zeiss Axio Imager Z1
Start Up Procedure
1. Remove dust cover from microscope
2. Toggle FluoArc lamp switch ON
3. Toggle yellow power bar switch ON
4. Toggle black power bar switch ON
5. Toggle Power Supply 230 box switch ON
6. If using ApoTome, toggle ApoTome box switch ON
7. Turn on computer
8. Log in:
- User Name: GuestUser
- Password: -leave blank-
9. Open Program: AxioVision
Shut Down Procedure
1. If used oil, gently remove oil from lens using neatly
folded dry lens paper
2. Lower stage and put 5x objective in place
3. Close AxioVision program
4. Shut down computer. Wait for computer to completely
turn off
5. If used ApoTome, move slider out to click stop
position1, then toggle ApoTome box switch OFF
6. Toggle Power Supply 230 box switch OFF
7. Toggle black power bar switch OFF
8. Toggle yellow power bar switch OFF
9. FluoArc Lamp:
- Regular work hours: Set intensity to 50% on control pad (F2),
and leave lamp ON
- Non-work hours: Turn OFF lamp when you are finished only if
no one else is using it after you and if the lamp has been on
for at least 30 minutes
Cover microscope with dust cover, but DON’T COVER THE
HOT LAMP HOUSING!!