See, what we do!

VARIOUS FACES OF BIOMEDICAL
ENGINEERING
Department of Biomedical Engineering
Faculty of Fundamental Technical Problems, Wroclaw University of Technology
www.ibp.pwr.edu.pl
Department of Biomedical Engineering
Faculty of Fundamental Technical Problems, Wroclaw University of Technology
www.ibp.pwr.edu.pl
Wrocław University of Technology WrUT is an inheritor of the tangible
property of the German Königliche Technische Hochschule Breslau and
the intellectual and research traditions of the Polish Lvov Polytechnic.
The university has been functioning under the current name since 1945. It
was established and organised after WWII by researchers from Lvov and
Warsaw.
Today, it belongs to the best technical universities in Poland – over 32.000
students study here under the guidance of 2.000 academic teachers, at the
12 faculties and the Department of Fundamental Studies, as well as in the
3 regional branches.
Department of Biomedical Engineering
Faculty of Fundamental Technical Problems, Wroclaw University of Technology
www.ibp.pwr.edu.pl
Department of Biomedical Engineering (former the Institute of
Biomedical Engineering and Instrumentation) belongs to the Faculty
of Fundamental Problems of Technology. Dozens of students study
the Biomedical Engineering at our Faculty (ca. 150 are enrolled
each year).
Seven research groups are actively involved in the research projects:
Bio-Optics Group, Laboratory of Biophysics of Macromolecular
Aggregates, Group of Bioinformatics and Biophysics of Nanopores,
Biomedical
Signal
Processing
Laboratory,
Group
of
Biospectroscopy, Group of Biomedical Electronics and
Measurements, Group of Computer Simulations of Biomedical
Systems.
Our research groups
Bio-Optics Group
Group leader:
Prof. Dr. Halina Podbielska
Optical systems and methods for quick bacteria characterization by optical methods. Antibacterial
materials. Photosensitive nanomaterials. Nanoscale measurements (AFM). Computer aided analysis of
medical images. Physcial medicine. Thermal imaging. Pattern recognition.
Laboratory of Biophysics of Macromolecular
Aggregates
Group leader: Prof. Dr. Marek Langner
Procedures to evaluate sample stability using calorimetric methods. Procedures to evaluate chemical
composition of complex solid materials using LIBS (Laser Induced Breakdown Spectroscopy). Size and
zeta-potential determination using DLS (Dynamic Light Scattering) method. Study of supramolecular
aggregates formation and stability for targeted drug delivery systems
Biological nanopores, such as electropores and protein ionic channels. Conductivity characteristics,
Group of Bioinformatics and Biophysics of
structure-function relations, and medical applications. Experimental study on electro-nanopores in
Nanopores
planar lipid membranes. Electrochemiotherapy of cancer cells in vitro. Computer modeling of
Group leader: Dr. hab. Małgorzata Kotulska, Assoc.
conductivity of various nanopores. Bioinformatical methods for modeling structure of membrane
Prof.
channel proteins.
Biomedical Signal Processing Laboratory
Group leader: Dr. hab. Robert D. Iskander, Assoc.
Prof.
Monitoring, modeling and analysis of intracranial pressure, arterial blood pressure and cerebral blood
flow velocity in patients with hydrocephalus. Modeling and clinical applications of the eye pupil
reactivity. Modeling and study of correlation between intraocular pressure (IOP) measurements and
geometry and biomechanical properties of the human eyeball.
Group of Biospectroscopy
Group leader: Prof. Dr. Małgorzata Komorowska
Studies of the influence of near infrared radiation on tissue, proteins, nucleotides, and aminoacids by
means of different spectroscopic methods. Red blood cells protection by IR irradiation. Spin labels ESR
spectroscopy. ATR-FTIR and Raman vibrational spectroscopy, UV-Vis and fluorescence spectroscopy.
Group of Biomedical Electronics and
Measurements
Group leader: Dr. hab. Zbigniew Moroń,
Assoc. Prof.
Bioelectronics. Biomedical measurements and instrumentation. Modeling of biomedical structures and
systems. Medical electronics. Medical navigation.
Group of Computer Simulations of Biomedical
Systems
Group leader: Dr hab. Krystian Kubica,
Assoc. Prof.
Membrane properties modeling by computer simulation technique . Study the interaction between
biologically active compounds and membranes.
Department of Biomedical Engineering
Faculty of Fundamental Technical Problems, Wroclaw University of Technology
www.ibp.pwr.edu.pl
Bio-optics and Biophotonics for Microbiological
Applications
Novel Perspectives on bacterial identification
by light diffraction
Novel Perspectives on the Characterization of
Species-Dependent Optical Signatures of Bacterial
Colonies by Digital Holography
BASIC CONCEPTS OF LABEL-FREE DETECTION
OF BACTERIA
BY LIGHT DIFFRACTION
Species/strains – dependentd
morphological and optical
properties of bacterial colonies
Morphology and metabolic
properties of bacteria cells
Pseudomonas vulgaris
Pseudomonas aeruginosa
Unique diffraction signatures of
bacterial colonies
Genotypic features of bacterial
cells affect the phenotypic
properties of bacterial cells
„BACTERIA FINGERPRINTS”
Staphylococcus aureus
2013
DEVELOPMENT OF THE OPTICAL
SYSTEM FOR BACTERIA
IDENTIFICATION
2010
2015
(1) Laser diode module, (2) Linear polarizer, (3) Amplitude filter, (4) Beam
expander, (5) Iris diaphragm, (6) Transforming lens, (7) Sample of bacterial
colonies in Petri Dish, (8) Holder, (9) CCD camera, (10) Computer.
I. Buzalewicz, A.Wieliczko, H. Podbielska, Influence of various growth
conditions on Fresnel diffraction patterns of bacteria colonies examined
in the optical system with converging spherical wave illumination, Optics
Express 19(22), 21768-21785, (2011)
Fresnel diffraction patterns analysis supported by
statistical
methods
enable
the
bacteria
classification with the accuracy over 98%.
Escherichia coli
(ATCC 35401)
Salmonella Enteritidis
(ATCC 13076)
Citrobacter freundii
(ATCC 43864)
Salmonella Typhimurium
(ATCC 13311)
Pseudomonas aeruginosa
(ATCC 27853)
Klebsiella pneumoniae
(ATCC 700603)
Proteus mirabilis
(ATCC 43071)
Staphylococcus intermedius
(PCM 2405)
The reference database contains over 10000
diffraction patterns of 30 bacterial species and over
15 strains.
A.Suchwałko, I. Buzalewicz, H. Podbielska, Bacteria identification in
an optical system with optimized diffraction pattern registration
condition supported by enhanced statistical analysis, Optics Express
22(21), 26312-26327, (2014)
A.Suchwałko, I. Buzalewicz, A. Wieliczko, H. Podbielska, Bacteria
species identification by the statistical analysis of bacterial colonies
Fresnel patterns, Optics Express 21(9), 11322-11337, (2013)
Bacillus subtilis
(ATCC 6633)
Enterococcus faecalis
(ATCC 51299)
Listeria monocytogenes
(ATCC 19115)
Staphylococcus aureus
(ATCC 43300)
A. Suchwałko, I. Buzalewicz, H. Podbielska, Statistical identification
of bacteria species, Microbial pathogens and strategies for
combating them: science, technology and education , vol. 1, A.
Mendez-Vilas (Eds.), Formatex Research Center (2013), s. 711-721
Digital holography for bacterial colonies characterization
Characterization of
species-dependent
bacterial colonies
profiles by digital
holography.
Demonstration that
bacterial colonies
exhibit analogical lightfocusing properties as
classical lenses.
I. Buzalewicz, K. Liżewski, M. Kujawińska, H. Podbielska, Degeneration of Fraunhofer
diffraction on bacterial colonies due to their light focusing properties examined in digital
holographic microscope system, Optics Express 21(22), 26493-26505, (2013)
Novel Perspectives on the Characterization of Species-Dependent
Optical Signatures of Bacterial Colonies by Digital Holography
In comparison with previous forward scattering/diffraction sensors it is
possible by digital holography to extract more species-dependent optical
signatures: digital holograms of bacterial colonies, amplitude and phase patterns of
bacterial colonies, amplitude and phase patterns of diffracted field on bacterial colonies in
all observation space.
Staphylococcus
intermedius
Escherichia coli
I. Buzalewicz, M. Kujawińska, W. Krauze, H. Podbielska, Novel Perspectives on the
Characterization of Species-Dependent Optical Signatures of Bacterial Colonies by Digital
Holography, PLoS One 11(3): e0150449.(2016) doi:10.1371/journal.pone.0150449
Biomedical Engineering in nanoscale
Biofunctionalization of stent surface
2013-2016 Grant FP7-PEOPLE-2012-IAPP”Antibody-functionalised cardiovascular
stents for improved biocompatibility and reduced restenosis”
Grant FP7 nr CP-FP 212533-2, “Electrically modified biomaterials’ surfaces: From Atom
to Application”, grant acronym: “BioElectricSurface”
11
Problems after coronary angioplasty
thrombosis
restenosis
SMC
restenosis
http://vueklar.com/MyImages/MRI%20of%20VueKlar%20stent%20with%20thrombosis.gif
Modification of stent surface
Collaboration with Balton and the Medical University of Wroclaw
Photoactive coating for implantable medical devices for interventional cardiology. Design
of photosensitive coating to prevent restenosis and thrombosis.
Stainless steel surface functionalization for immobilization of antibody fragments for cardiovascular applications.
Foerster A, Hołowacz I, Sunil Kumar GB, Anandakumar S, Wall JG, Wawrzyńska M, Paprocka M, Kantor A,
Kraskiewicz H, Olsztyńska-Janus S, Hinder SJ, Bialy D, Podbielska H, Kopaczyńska M.
J Biomed Mater Res A. 2016 Apr;104(4):821-32
Immunofluorescence images of endothelial cells after 4h
adhesion and 5 days of proliferation on bar stainless
steel and titania coatings
The implantation of photoactive stent
Biocompatibile coating has potential activity against restenosis.
The photoactive stent was produced in prototype series by Balton, tested in vitro and in
vivo and is currently preparing to undertake clinical trials.
Angiografia
Patent P. 395888.
Histopatologia
OCT
Stent in the vessel
In vivo study
Explanted stent
Patent Application P.412016
The photoactive formulation of
nanoliposomes as a new strategy in the
photodynamic therapy of unstable
atherosclerotic plaque
2014– 2017 Grant NCN, „The photoactive formulation of nanoliposomes as a new
strategy in the photodynamic therapy of unstable atherosclerotic plaque”.
17
Application of photosensitizing drug in a
liposomal nanoformulation
100 nm
TEM image of liposomal
nanoformulation with encapsulated
photoactive drug
Colocalization of liposomal
nanoformulation with encapsulated
photoactive drug in the cells 18
The prototype system for intravascular PDT
19
Nanostructural changes in the cellular
structures induced by drugs used in
chemotherapy of malignant tumors
2012-2015 Grant NCBiR, “Optical tweezers in biomedical engineering”
20
DNA-drug complexes
intercalation
electrostatic
interaction
groove binding
Nanostructural changes of DNA caused by doxorubicin
AFM images
DNA
Anthracycline antibiotic :
 doksorubicin,
 daunorubicin,
 epirubicin;
DNA + DOX
Nanostructural changes of DNA caused by
aminoglycosides
AFM images: A) free DNA on mica surface, B) toroidal structures
caused by tobramycin, C) DNA strands degradation caused by
kanamycin
Molecular model of the toroidal structures
New strategies of targeted molecular therapy
Selective condensation of DNA by aminoglycoside antibiotics.
Kopaczynska M, Schulz A, Fraczkowska K, Kraszewski S, Podbielska H, Fuhrhop JH.
Eur Biophys J. 2015 Dec 8.
The studies of the mechanical properties of the
cell structures induced by drugs used in
chemotherapy of malignant tumors
2012-2015 Grant NCBiR, “Optical tweezers in biomedical engineering”
25
Optical tweezers in mechanical studies of nanomaterials
Optical tweezers in oncology
Mechanical properties study of blastic stem
cells of acute myelogenous leukemia in
optical trap
Blood smear patient with
acute myeloid leukemia
Collaboration with Medical University of Wroclaw. Studies on biomechanical
properties of the blastic cell membranes of acute myeloid leukemia, comparison
between healthy blood cells and aggressive cancer cells.
27
Nanomanipulation of the cells by using optical
tweezers
Stem cells of acute myelogenous
leukemia blasts
Erythrocyte in the trap
28
Bio-optics and Biophotonics for
Predictive, Preventive and
Personalized Medicine
Novel Perspectives of IR imaging’ applications
for personalized physiotherapeutic approach
Novel Perspectives of IR imaging for early
detection and therapy monitoring of
lipodystrophy
INFRARED IMAGING IN
MEDICINE AND
PHYSIOTHERAPY
THERMOVISION (THERMOGRAPHY) 
measurement technique which enables
registration and visualization of Infra
Red radiation emitted by each body
which temperature is above absolute
zero.
Image of the body’s surface
temperature distribution, obtained
as a result of IR radiation
recording, is called thermogram.
Healthy patient on the right and patient with
thromboembolism and inflammatory process on the
left
Patient with Reynaud disease
The following stages of coronary circulation after
LIMA-LAD anastomosis
Patient with spine disease
DETECTING OF EARLY STAGES
OF
LIPODYSTROPHY
AND ASSESSMENT OF TREATMENT
PROGRESS
Nürnberger Müller
scale
US
G
Cellulite
diagnostics
CT
Healthy tissue
Stage 0
Thermograp
hy
NMR
Dermatoscopy
Patient with
Stage 2
Nürnberger-Müller scale (1978)
Stages of cellulite
Patient with
Stage 1
Patient with
Stage 3
THERMAANALYZER – NEW SOFTWARE FOR DIAGNOSTIC,
REAL-TIME THERAPY MONITORING AND TREATMENT
PROGRESS’ ASSESSMENT BY USE THERMOVISION
Open file
Select ROI /Set parametrs/
Analyze
View results
in new window
ThermaAnalyzer –
working with the
program
Calculation the spots
number and size
THERMAANALYZER – LIPODYSTROPHY TREATMENT MONITORING
Patient nr 10, initially stage 3
Before treatment
After 4 weeks
treatment
Patient nr 8, initially stage 1
Before treatment
After 4 weeks
treatment
Persons with higher
degree of cellulite
have more irregular
superficial
temperature
distribution seen as
the spots on the
thermal image.
Thermal imaging can
be
an
objective
method
for
lipodystrophy
diagnosis and therapy
monitoring.
SCIENTIFIC OBJECTIVES OF THE RESEARCH:
1. Establishment of personalized procedures for
monotherapies, based on such parameters like:
 age,
 gender,
 BMI,
 parameters of physical agents
2. Establishment of personalized procedures for
polytherapies, including such parameters like:
 treatment order,
 optimal break between treatments
 parameters of physical agents
Person 10
Person 1
Person 17
Person 5
Person 13
RESEARCH:
1. Personalization of physical treatment is not a trivial task, as a proper planning of the procedures, especially in
case of joined therapies, requires an extensive and profound knowledge of physiological and pathological
conditions of the body's response to stimuli and regular tracking of the organism’s reaction.
2. Physical stimuli should cause specific therapeutic reaction
3. There are still no comprehensive studies of the different physical procedures, depending on such parameters as
patient’s age, gender, BMI, dose, stimuli duration as well as the application’s order.
MONITORING OF PHYSIOTHERAPEUTIC
POLYTHERAPIES AND MONOTHERAPIES
Thermogram
before treatment
Patient
No 16
(Woman)
After
cryotherapy
After 15
minutes
After
ultrasounds
After 30
minutes
Our study demonstrated that older persons do not react on the
physical agents in form of cooling or heating in the same way
as the young individuals.
A significant differences can be seen just after applying the
second treatment in both cases DD-Soll and Soll-DD
polytherapies. However, the thermal effect is short and no
significant differences were observed after 15 and 30 minutes
A significant difference can be observed in case of US-CRYO
versus CRYO-US polytherapies. In case of US-CRYO order the
cooling effect lasts longer (up to 30 min) whereas in case of
CRYO-US the thermal effect is shorter ( up to 15 minutes)
These findings have practical significance, especially when the
treatment procedure is performed in order to facilitate the
kinesiotherapy and physical exercises.
Boerner Ewa*, Bauer Joanna, Kuczkowska Magdalena*, Podbielska Halina, Ratajczak B*: Comparison of the skin surface temperature on the front of thigh
after application of combined red-IR radiation and diadynamic currents executed in a different sequence Journal of Thermal Analysis and Calorimetry. 2015,
vol. 120, nr 1, s. 921-928, http://dx.doi.org/10.1007/s10973-015-4545-9
Boerner Ewa*, Bauer Joanna, Ratajczak B*, Dereń Ewelina*, Podbielska Halina: Application of thermovision for analysis of superficial temperature distribution
changes after physiotherapy Journal of Thermal Analysis and Calorimetry. 2015, vol. 120, nr 1, s. 261-267, http://dx.doi.org/10.1007/s10973-014-4026-6
Laboratory for Biophysics of Macromolecular Aggregates
LAB FACILITY:
-
Steady-state and time resolved
fluorescence,
Dynamic light scattering,
HPLC (UV/VIS, FLUO, ELSD),
Stopped-flow apparatus,
UV/VIS spectroscopy,
LIBS,
Ultrafiltration,
Differential Scanning Calorimetry,
Isothermal Titration Calorimetry
prof. Marek Langner
Pl. Grunwaldzki 13, bud. D1 pok. 8A
phone: +48 71 320 41 55
E-mail: [email protected]
www.lbam.pwr.edu.pl
Magda Przybyło, PhD
Sebastian Kraszewski PhD
Maciej Łukawski, MSc
Kamila Szostak, MSc
Dominik Drabik, MSc
Laboratory for Biophysics of
Macromolecular Aggregates
BASIC RESEARCH : BIOPHYSICS OF LIPID MEMBRANE
Studies on lipid membrane permeability
Experimental studies of water transport through
the lipid membrane – development of new
method based on stopped – flow technique
Przybylo M, Drabik D, Lukawski M, Langner M. Effect of Monovalent Anions on Water
Transmembrane Transport. Journal of Physical Chemistry B. 2014;118(39):11470-11479. A
Supported by Molecular Dynamic Simulations
Lis M, Wizert A. Przybylo M. Langner M. Swiatek J. Jungwirth P.
Cwiklik Ł. The effect of lipid oxidation on the water permeability of
phospholipids bilayers Phys. Chem. Chem. Phys., 2011, 13, 17555–
17563
Determination of lipid bilayer bending properties by vesicle
fluctuation analysis based on confocal imaging
Drabik, D; Przybylo, M; Chodaczek, G; Iglic, A; Langner, M The modified fluorescence
based vesicle fluctuation spectroscopy technique for determination of lipid bilayer bending
properties Biochimica et Biophysica Acta – Biomembranes 2015; 1858 (2), 244-252
Determination of lipid diffusion coefficient by Fluorescence
Correlation Spectroscopy
Drabik D, Przybyło M., Sikorski A., Langner M., The Effect of a Fluorophore PhotoPhysics on the Lipid Vesicle Diffusion Coefficient Studied by Fluorescence Correlation
Spectroscopy, Journal of Fluorescence DOI 10.1007/s10895-015-1752-5.
Studies of lipid membrane mechanics by microscopic imaging
www.brainlab.pwr.edu
.pl
Brain Research Lab
Joint time-frequency analysis
of dynamic cerebral autoregulation
Research supported by National Science Centre, Poland
(no. DEC-2013/10/E/ST7/00117)
Non-invasive measurements:
 Arterial Blood Pressure – Finapres finger plethysmograph (Finometer®
MIDI, FMS Medical Systems)
 Cerebral Blood Flow Velocity – Transcranial Doppler ultrasonography
(Doppler-Box™ X DWL)
 End tidal CO2 – capnograph (RespSense™, NONIN)
 ECG – three lead (ECG module, FMS Medical Systems)
Experimental Procedure


Hypercapnia is induced by increasing the
respiratory dead space by attaching
plastic sack.
Hypercapnia is used as a model of
cerebral autoregulation impairment.
Results: Exemplary Subject
Cerebral autoregulation is less effective in hypercapnia (higher T-F coherence,
lower T-F phase shift between arterial blood pressure and cerebral blood flow
velocity )
hypercapnia
normocapnia
time-frequency coherence= 0.44
time-frequency coherence = 0.36
0.07
1
0.06
0.8
0.05
0.6
0.04
0.4
0.2
0.06
0.8
0.05
0.6
0.04
0.4
0.03
0.2
0.03
0
0.02
0
0.02
0
1
2
3
Time (minutes)
4
5
time frequency phase shift = 35.5°
Frequency (Hz)
Frequency (Hz)
0.07
1
2
3
Time (minutes)
4
time-frequency phase shift =
17.9°
0
Research
www.brainlab.pwr.edu
.pl
Multimodal monitoring of biosignals in brain injury patients
Decomplexification of biosignals in brain pathology
Complexity of T-F representations of arterial blood pressue (ABP) and cerebral
blood flow velocity (CBFV) is lower during vasospasm than before vasospasm in
patients after subarachnoid haemorrhage (SAH)
1st day after SAH (before vasospasm)
ABP
Renyi entropy=51.05
CBFV (side of vasospasm)
Renyi entropy=38.62
10th day after SAH (during
vasospasm)
ABP
Renyi entropy=27.58
CBFV (side of vasospasm)
Renyi entropy=29.98


Ocular dicrotism, a double-peak shaped feature in corneal pulsation, is a
newly observed phenomenon (2015). It was found to increase its prevalence
with age and with glaucoma progression.
Ocular dicrotic pulse, characterised by two corneal pulsations with each
cardiac cycle, was detected with innovative ultrasonic transducers.
Ocular
dicrotism
New instrument for measuring corneo-scleral topography

Wide coverage
range
Typical coverage by a
standard Placido disk
videokeratoscope
Fourier Profilometry
topographer
Fluroescein needs to be
instilled into the eye
CURRENT EUROPEAN PROJECTS
Prof. R. Iskander
H2020-MSCA-ITN-2014 EDEN – European Dry Eye Network, ID 642760,
European Commission, start: 01-10-2014
FP7-PEOPLE-2013-ITN AGEYE – Aging Eye, ID MC608049, European
Commission, start: 01-10-2013
Prof. H. Podbielska
FP7- PEOPLE-2012-IAPP EU Industry-Academia Partnerships and
Pathways (IAPP) EPICSTENT Antibody-functionalised cardiovascular
stents for improved biocompatibility and reduced restenosis, start: 01-052013
Department of Biomedical Engineering
Faculty of Fundamental Technical Problems, Wroclaw University of Technology
www.ibp.pwr.edu.pl
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