docEssential Information for the Aerospray® Gram Slide Stainer
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Essential Information for the Aerospray® Gram Slide Stainer
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p. Troubleshooting
If you suspect a problem with reagent delivery, diagnose the problem by assessing the performance of each spray
nozzle.
Press the prime buttons and observe each nozzle for uniform dispersion, pattern shape, and direction of spray. Test
spray pattern and spray volume to determine which nozzle is not functioning properly. Use the instructions under
Disassembly and Cleaning to clean any nozzle that does not appear to function normally.
Disassembly and Cleaning
The automatic clean cycle eliminates the evaporative accumulation of reagent solute in the nozzle system. Foreign
material may, however, get into the system and eventually plug the nozzle. If this happens, manually disassemble
and clean the spray nozzle as follows:
1. Slide the nozzle tool over the spray nozzle and turn counter-clockwise to loosen and remove.
2. While taking the nozzle apart, become familiar with the nozzle, its parts and assembly.
NOTE: The following procedure requires using the Nozzle Maintenance Kit. Use the kit to prevent
mixing nozzles or nozzle parts. When removing Aerospray nozzle(s), be sure to return all nozzles to
their original positions after cleaning. This helps ensure consistent staining performance.
3. Hold the spray nozzle with the nozzle tool and insert the 5/32 nozzle hex wrench into the compression screw.
Turn counter-clockwise to loosen and remove. DO NOT DROP COMPRESSION SCREW OR SWIRL
CONE.
4. Remove the o-ring.
NOTE: Excessive force used to loosen and remove the compression screw can damage the plastic
nozzle tool. If the compression screw cannot be readily loosened, soak the nozzle in methanol, ethanol,
or prepared SS-029C to remove residue. If the problem continues, use light penetrating oil and a 5/8inch wrench to loosen the nozzle.
CAUTION! Do not use hardened metal instruments to clean or scrape nozzle components.
5. Place disassembled metal nozzle parts in one of the provided 50 mL centrifuge tubes.
6. Fill tube to 25 mL mark with methanol, ethanol, or prepared SS-029C.
7. Cap tube, agitate, and then soak parts until clean.
WARNING! Always wear protective clothing and eye protection when using the SS-029C.
Dispose of used cleaning solution in the correct manner.
8.
9.
10.
11.
Agitate again, and pour off the solution.
Use the provided cleaning wire (AC-059) to clean the nozzle orifice.
Remove any material in the swirl cone grooves by sliding the edge of a piece of paper through each groove.
Inspect nozzle parts to ensure they are completely clean. Repeat soaking if necessary.
NOTE: To prevent mixing nozzle parts, use the tube stand inside the Nozzle Maintenance Kit. Place
tube containing nozzle parts in the punched hole that corresponds with the reagent line that the nozzle
came from.
12. Rinse parts in the tube with deionized water. Continue rinsing until cleaning solution is completely removed
from parts and tube.
13. Rinse parts in the tube with methanol, ethanol, or prepared SS-029C, then remove parts from tube.
14. Reassemble nozzles and return nozzles to their original locations in the stainer.
DOC-00309-ENA.doc
Page 23 of 27
! Wescor, Inc
Nozzle Components
Reassembly:
1. Use a swab to apply a small amount of silicone lubricant (SS-103, included with the instrument) to the threads
of the compression screw to prevent binding.
2. Insert the swirl cone into the compression screw. Hold this assembly and nozzle housing in a vertical position.
HOLD THIS POSITION UNTIL THE NOZZLE REASSEMBLY IS COMPLETED.
3. Insert the long end of the hex wrench into the screw up to the stop. Turn the compression screw into the nozzle
housing. Tighten firmly with the nozzle tool and hex wrench. If assembled correctly, the compression screw
should screw into the nozzle housing approximately ! inch.
4. Replace the O-ring.
5. Install the spray nozzle by turning it in a clockwise direction. Be sure to return each nozzle to its original
position. Use the nozzle tool and tighten to a snug fit. DO NOT OVERTIGHTEN.
6. Prime the nozzle and check spray pattern and spray volume before staining.
i.
Safe Waste Disposal
Disposal of fluids may be governed by local regulations, consult material safety data sheets.
q. Setup and Pre-use Procedures (8.7o)
i.
Installing Drain Tube
Place the slide stainer on a level counter surface near a sink or suitable drain or waste container. Attach the drain
tube to the drain port on the back lower right of the unit. Route the tube to a drain or vented waste container. Make
certain the tube remains below the drain port. Run the tube continuously downward to the drain or waste container
so that liquid cannot be trapped in the line. Keep the tube as short as possible, no longer than 1.8 meters.
NOTE: Do not submerge the end of the drain tube in liquid (this interferes with draining).
ii.
1.
2.
3.
Connecting Power
The power switch is located on the back panel in the power entry module.
Make certain the switch is OFF (O).
Plug the female end of the power cord into the power entry module (85 to 264 Volts AC).
NOTE: We recommend utilization of a power line surge protector to isolate the instrument from
spikes and surges.
4.
5.
Plug the male end of the power cord into a grounded power outlet.
Turn the power switch ON (I). The power indicator on the front panel should now be on. The display initially
shows the software version.
NOTE: Leave the power on except when the instrument is serviced or moved.
iii.
Install all reagent bottles
Place the reagent bottles front to back in the following order:
(A) Decolorizer with Counterstain
(B) Iodine
(C) Crystal Violet
(D) Deionized Water
(E) Anhydrous Methanol or Ethanol (" 0.5% water)
DOC-00309-ENA.doc
Page 24 of 27
! Wescor, Inc
WARNING! Reagents used in the Aerospray Stainer contain moderately hazardous chemicals that
require care in handling. Always use appropriate safety measures including gloves and eye
protection when handling reagents.
CAUTION! To avoid severe damage, never use reagents containing organic solvents in this
instrument unless they are supplied by Wescor or specified in official Wescor formulation
instructions.
Remove the cap from each bottle. Remove the central portion of the seal.
Insert the dip tubes into the reagent bottles and screw on the ring caps.
iv.
Aerospray Nozzle Maintenance Kit
The Aerospray Nozzle Maintenance Kit (AC-075) is provided to help maintain optimum performance from the
reagent delivery system. The kit contains tools and equipment to clean the nozzles, check reagent delivery and to
prime the instrument.
v.
Prime all reagent lines
For peak stainer performance, thoroughly purge and prime each reagent delivery line using the following
instructions. This procedure uses approximately 250 mL of reagent per line. Extra reagent is included with the new
instrument for this purpose.
Remove each spray nozzle with the provided nozzle tool by turning it counterclockwise. Note the location of each
nozzle (so you can return it to the original location).
Place a carousel in the stainer to prevent stain from entering the motor shaft. Briefly press each prime button. Stain
should appear within 10 seconds. If no stain appears within 10 seconds there may be an air lock in the line.
Immediately stop priming. Use Reagent Pump Priming Tool (AC-069), part of Nozzle Maintenance Kit, to remove
the air lock.
If stain begins to appear within 10 seconds (or once an air lock is resolved), prime all reagent lines. This can be done
manually or by using Prime Lines or Volume Test functions. When properly primed, a steady stream of reagent (no
sputtering or breaks) flows from each nozzle receptacle while pressing the corresponding prime button.
WARNING! Never operate a dry pump longer than 10 seconds.
Simultaneously Priming All Lines
Press VOLUME TEST. Press 4 on the keypad to prime all the reagent lines. The pumps run for 60 seconds. After
priming reagent lines, replace the nozzles. Return each nozzle to its original location for consistent staining
performance.
Priming Individual Lines
Prime manually for 60 seconds or press VOLUME TEST. Press 2 on the Keypad. Press the desired prime button.
The pump for that position runs for 20 seconds. Repeat twice more to achieve 60 seconds of flow.
After priming all reagent lines, replace the nozzles. Return each nozzle to its original location for consistent staining
performance.
POWER
With the nozzles installed, repeat the priming sequence above. Use a carousel in the instrument. A fine cone of spray
should come from each nozzle. Run the Spray Volume and Spray Pattern tests. After verifying nozzle performance,
run the CLEAN cycle.
vi.
Run the CLEAN cycle
The CLEAN cycle is a two-stage process that uses methanol or ethanol to purge the reagent nozzles of precipitates
and debris, then reprimes reagents into the nozzles. Each stage of the cycle requires user input to continue the
DOC-00309-ENA.doc
Page 25 of 27
! Wescor, Inc
process. This allows delaying the second stage indefinitely. The steps described below are for initial setup (see
following Note), routine cleaning of the instrument, or to keep the nozzles clean while the instrument is idle. We
recommend frequent use (at least once per shift) of the CLEAN cycle to ensure reliable nozzle performance.
NOTE: Run the following CLEAN cycle routine twice during initial setup, to help remove any air bubbles
from the reagent lines and ready the instrument for slide staining.
•
•
•
•
•
•
Place an empty carousel in the instrument and close the lid.
Push CLEAN. This sprays methanol or ethanol (Reagent E) through the nozzles and onto the carousel. When
the cycle is completed the display reads: PRESS CLEAN TO REPRIME.
Wipe nozzle orifices to remove all residual liquid.
At this point, the instrument “stands by” with methanol or ethanol remaining in the nozzles until pressing
CLEAN again. The instrument can be left in this standby mode for extended periods to prevent clogging while
the stainer is idle.
If not staining slides immediately, leave the instrument in standby mode until ready to stain. This is particularly
important if the instrument will be idle for more than 4 hours. Later, when ready to stain slides, make sure there
is a carousel in the instrument, then:
Press CLEAN again. This reprimes reagent into the nozzles, making the instrument ready for staining.
CAUTION! Do not place any carousel loaded with specimens in the instrument while CLEAN TO
REPRIME appears on the display. Specimens will be damaged if they contact excess reagents
sprayed from the nozzles when you press CLEAN.
NOTE: Pressing STOP during the clean or reprime cycles causes a warning to be displayed until a
complete Clean and Reprime cycle is completed.
r. Process for Cleaning, Decontamination (8.7q)
Under normal clinical use the Aerospray" Gram Slide Stainer/Cytocentrifuge poses very little risk of biological infection
to laboratory workers. The stainer is essentially an environmental surface, which should be kept clean. Only low-level
disinfection is required.
Biological contamination occurs only when specimens slough off the slides during staining. These tend to be removed by
the continual flow of reagents through the instrument. In addition, the reagents used in the staining and cleaning process
are suitable for low to intermediate level disinfection. The stainer is self-cleaning under normal use, but cannot be
considered decontaminated. The stainer exterior can also be contaminated by touching with contaminated gloves and
requires routine surface cleaning.
For additional decontamination, the following procedures provide low to intermediate disinfection. If the stainer is
contaminated by unusually hazardous or disinfection resistant organisms, further treatment with appropriate procedures
may be necessary. If sending the stainer to Wescor for service or repair, contact Wescor for current decontamination and
shipping instructions.
1.
2.
Mask the lid latch and locking pin holes with waterproof tape to protect the stainer Interior.
Place the stainer in a biological safety hood or well-ventilated area. Use prudent safety precautions including hand
and eye protection.
CAUTION! Do not flood the stainer bowl by overloading the drain. Never allow fluid to rise above
the base of the drive hub. Do not spray fluids near openings in the stainer housing that lets fluid
into the instrument interior. This can cause severe damage.
3.
4.
5.
Spray inner bowl and inner lid with disinfectant detergent such as a 10% bleach solution or Wescor’s
Decontamination Solution (SS-133).
Repeat spray treatment every 2 or 3 minutes. Leave solution on surfaces for approximately 10 minutes. Do not allow
cleaning solutions to dry on the stainer surfaces.
Rinse inner bowl and lid thoroughly with tap water.
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6.
7.
8.
9.
With the stainer lid closed, apply decontamination solution to a cloth. Wipe the exterior surfaces of the stainer. Do
not flood the display panel with excessive moisture. Any moisture that seeps through could damage the internal
electronics. Repeat wipe down every 2 or 3 minutes for about 10 minutes total.
Remove decontamination solution by thoroughly wiping surfaces with a cloth soaked in tap water.
Immerse or generously spray the carousel and lid with disinfectant detergent. Allow to sit for 20 to 30 minutes. Do
not autoclave the carousel or lid.
Thoroughly rinse the carousel and lid with tap water.
NOTE: These decontamination procedures are for routine use only. If shipping the stainer to Wescor for
repair or service, contact Wescor ’s Service department for a current copy of the decontamination and
shipping instructions before preparing and shipping the stainer. Shipping stainers without
decontaminating them according to these instructions is dangerous to service personnel and results in a
significant decontamination charge.
s. Environmental Specifications and Precautions (8.7r)
Device conforms to EMC directive 89/336/EC. Using this instrument in a manner not specified by Wescor may impair the
safety protection designed into the equipment and may lead to injury.
t. Disposal of Device (8.7s)
The device is subject to the WEEE Directive 2002/96/EC and cannot be disposed of in a normal landfill. Instead, the
equipment must be disposed of either by routing to an authorized local facility approved for handling hazardous materials,
or returning the equipment to Wescor, Inc.
END
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