AUTOFIND S OPEN rev111206 low-res

Transcription

autoFIND S open - User Manual
autoFIND S
open
Preparative station for microFIND®
AUTOFIND S OPEN_rev111206.doc
i
This manual contains information about the AUTOFIND S OPEN preparative station
for MicroFIND®.
The information contained in this document is subject to change without notice.
This document is for information purposes only. The Manufacturer shall not be
liable for errors contained herein or for incidental or consequential damages in
connection with the furnishing, performance, or use of this material. Risks derived
from improper use of this manual lie exclusively with final user.
Unless otherwise specified, each reference to names, or belongings is purely
casual and has the purpose to illustrate the product here described.
All rights reserved. Reproduction, adaptation, or translation without prior written
permission is prohibited, except as allowed under copyright laws.
All rights are reserved.
Version: a
ii
06/12/2011
INDEX
1.
INTRODUCTION ............................................................................................. 1-1
1.1 Intended use and restrictions ...................................................................... 1-1
1.2 Symbol table ................................................................................................. 1-2
1.3 Warning ........................................................................................................ 1-3
1.4 Manual Structure .......................................................................................... 1-6
1.5 Applicable rules ............................................................................................ 1-7
1.6 General specifications ................................................................................... 1-7
1.7 Technical specifications ................................................................................ 1-9
2.
EQUIPMENT DESCRIPTION ............................................................................ 2-1
2.1 Parts definition ............................................................................................. 2-1
2.1.1 microFIND® area ....................................................................................... 2-2
2.1.2 Reagent vial areas ..................................................................................... 2-2
2.1.3 Syringe location ........................................................................................ 2-3
2.1.4 Connection panel ....................................................................................... 2-7
2.1.5 Control panel ............................................................................................. 2-8
2.1.6 Wash nozzle .............................................................................................. 2-9
2.1.7 Cover ......................................................................................................... 2-9
2.2 Moving parts ................................................................................................. 2-9
2.3 Control panel description ............................................................................. 2-10
2.3.1 Typographic notes ..................................................................................... 210
2.3.2 Directional keys ......................................................................................... 210
2.3.3 STOP, START, EXIT and ENTER keys ......................................................... 211
2.3.4 SET, MET and SEQ keys ............................................................................. 211
3.
INSTALLATION ............................................................................................... 3-1
3.1 Location ........................................................................................................ 3-1
3.2 Preliminary operations ................................................................................. 3-2
3.3 Unit assembly ............................................................................................... 3-2
3.4 Installation ................................................................................................... 3-2
3.4.1 Voltage control and set-up ........................................................................ 3-4
3.4.2 Blocks removal .......................................................................................... 3-5
3.4.3 Tubes positioning ...................................................................................... 3-6
3.4.4 Electrical connections ............................................................................... 3-6
3.4.5 Start-Up ..................................................................................................... 3-6
3.5 Switch off ...................................................................................................... 3-7
4.
SET UP ............................................................................................................ 4-1
4.1 General ......................................................................................................... 4-1
4.2 Setup Mode ................................................................................................... 4-1
4.3 Manual Operations submenu ........................................................................ 4-2
4.4 Submenu Syringe Setup ............................................................................... 4-4
4.5 Syringe installation ....................................................................................... 4-5
4.5.1 First installation ........................................................................................ 4-5
4.5.2 Removal .................................................................................................... 4-6
4.5.3 Substitution ............................................................................................... 4-7
4.5.4 Changing the Syringe holder ..................................................................... 4-7
5.
OPERATIONS .................................................................................................. 5-1
5.1 Installing and removing reagent vials .......................................................... 5-1
5.2 Installing and removing the microFIND® area cover ................................... 5-2
iii
5.3 Running ........................................................................................................ 5-2
6.
TROUBLESHOOTING ...................................................................................... 6-1
6.1 Preliminary ................................................................................................... 6-1
6.2 Sensor touch trouble .................................................................................... 6-1
Running errors ...................................................................................................... 6-1
6.2.1 Missing objects .......................................................................................... 6-1
6.2.2 Obstacle..................................................................................................... 6-2
6.2.3 Blocked movement .................................................................................... 6-3
6.3 Syringe needle damage ................................................................................ 6-5
7.
MAINTENANCE ............................................................................................... 7-1
7.1 Ordinary maintenance .................................................................................. 7-1
7.1.1 Washing procedure ................................................................................... 7-2
7.1.2 External cleaning of cabinet and air outlet opening ................................. 7-2
7.1.3 Replacement of the blotting paper inside the cover ................................. 7-2
7.2 Extraordinary maintenance .......................................................................... 7-3
8.
RECYCLING AND DISPOSAL ........................................................................... 8-4
8.1 Preparer uninstall ......................................................................................... 8-4
8.2 Preparer transport ........................................................................................ 8-5
8.3 Preparer disuse ............................................................................................. 8-5
8.4 Disposal instructions .................................................................................... 8-6
9.
Appendix A CONSUMABLES ............................................................................ 9-1
9.1 Syringes ........................................................................................................ 9-1
9.2 Reagent vials ................................................................................................ 9-1
9.3 Probe vials .................................................................................................... 9-1
9.4 Cover blotting paper ..................................................................................... 9-2
9.5 Flasks ............................................................................................................ 9-2
9.6 Manual syringe ............................................................................................. 9-2
10. Appendix B FLOW DIAGRAMS ........................................................................ 10-1
10.1 Setup mode ................................................................................................... 10-1
11. Appendix C SCREEN LIST ............................................................................... 11-1
11.1 Start-Up ........................................................................................................ 11-1
11.2 Run Time Menu ............................................................................................. 11-1
11.3 Setup ............................................................................................................. 11-2
11.4 Errors ............................................................................................................ 11-3
12. Appendix D INTERCONNECTION PANEL ........................................................ 12-1
12.1 Output interface ........................................................................................... 12-1
12.2 RS232 interface ............................................................................................ 12-3
FIGURES INDEX
SCREEN INDEX
iv
I
II
Chapter
1
1. INTRODUCTION
1.1 Intended use and restrictions
autoFIND S open is a preparer used to automate some phases of the FISH
technique (fluorescence in situ hybridization). However, the preparer and the
relative software are not intended to give any diagnostic information or result;
they haven’t to be used as a part of a specific diagnostic test in vitro and they are
designed only for use in the research field.
The preparer must be used in a professional environment and only by properly
trained users, which know the specific hazards of this technique and of its specific
application. The preparer must be used according to the regulations in force
regarding the safety in testing laboratories.
Other techniques or areas of application are possible. In particular, it can be used
in applications where the sample preparation phase must be automated.
The samples treated by the preparer are biologic samples, while the reagents and
washing liquids can be various according to the application.
The operator is responsible for the definition of the features and of the necessary
performances for the application in which the preparer is used. Furthermore the
operator is responsible for the sample traceability and of the correctness of the
operations carried out by the preparer.
Every possible mixture or omission of a component cannot be recognized or
previously prevented.
The operator that will use the preparer must be trained about the used
substances, their hazard, their use and their proper disposal. For the correct use
of the used substances, refer to chemicals safety data sheets (MSDS) and to the
regulations, laws, directives and safety decrees currently in force that regulate the
behaviour inside the testing laboratories.
The insertion and positioning of the reagents, slides and pads, the insertion of
syringes and washing solvents and all the other preparation activities are under
the sole responsibility of the user. The user must operate in accordance with the
law, in particular the ones concerning the safety of testing laboratories and the
accident prevention.
1-1
The operator must be trained to operate in accordance with the safety standards
for the laboratories. The preparer does not have specific protection mechanisms
against substances that are corrosive, potentially infectious, toxic and radioactive
or against other substances that can be hazardous for health. However, when the
preparer is used, because of the handled substances, the user could need to use
individual protection measures such as gloves, masks and goggles; the use of such
devices must comply with the regulations currently in force regarding the safety of
testing laboratories.
Anyhow, the use of the preparer for any technique invasive for humans or animals
is explicitly prohibited.
The preparer must be used only in the manner described in the use instructions.
Any other use may affect the preparer and the operator safety.
The manufacturer is not responsible for damages caused, even only in part, by
improper use of the preparer, by unauthorized modification of it, by a different or
missing execution of the procedures described in this manual, or by the use of the
preparer violating the applicable laws and regulations.
The preparer has been designed and tested in accordance with current safety
standards.
1.2 Symbol table
Please read carefully this table before using your preparer. These symbols are
used in this manual.
do not touch: action forbidden
high voltage
warning: check the manual
warning: indicates pinch, crush and cut hazard
Indicates the possible presence of
hazardous substances
1-2
hot surface
1.3 Warning
Read the following warnings before unpacking or using the unit.
See the paragraph 1.1“Intended use and restrictions “ and be sure to
have understood.
See Declaration of CE Compliance enclosed with the preparer.
Before removing the preparer from the box please read carefully
paragraphs 3.2 “Preliminary operations” and 3.3 “Unit assembly “.
Before switching ON the preparer check the correct voltage.
Warning: pinch, crush and cut hazard during movimentation.
Electrical Hazards. Every laboratory instrument has specific hazards, so
ensure to read and comply with the following precautions.
Only use fuses of the type and current rating specified. Do not use
repaired fuses and do not short-circuit the fuse holder.
The supplied power cord must be inserted into a power outlet with a
protective earth contact (ground). When using an extension cord,
make sure that the cord also has an earth contact.
Do not change the external or internal grounding connections.
Tampering with or disconnecting these connections could endanger
you and/or damage the preparer.
The product is designed and tested in accordance with international
safety standards.
You do not need to make any changes to the electrical connections or
to the chassis of the preparer to ensure safe operation
1-3
Do not turn the preparer on if you suspect that it has incurred any
kind of electrical damage or if it shows visible signs of damage. If, for
any reason, the safe protections were compromised or if the preparer
shows visible signs of damage, disconnect the power cord and contact
the manufacturer. The power cord connector is considered the
disconnecting device of the product. Electrical damage may have
occurred if the preparer shows visible signs of damage, or has been
transported under severe stress.
As concern preparer recycling and disposal the following operations
have to be carried out: switch off the preparer and remove syringe,
samples and reagents, eliminating any contamination on the
instrument in accordance with national and international safety
regulations.
Damage can also result if the preparer is stored for prolonged periods
under unfavourable conditions (e.g. subjected to heat, water, etc.).
Do not place the preparer so that it is difficult to handle the
disconnecting device of the preparer.
Always disconnect the power cord before attempting any type of
maintenance.
Capacitors inside the preparer may still be charged even if the
preparer is turned off.
The preparer includes a number of integrated circuits. These circuits
may be damaged if exposed to excessive line voltage fluctuations
and/or power surges.
If, for technical reasons, it is necessary to work on preparer parts,
which are accessible only using a tool, contact the Technical
Assistance Centre. Violation of this directive implies the immediate
invalidation of the warranty and service contract. These restrictions
don't affect normal operations as syringe installation and removing.
Avoid contact with the preparer while it is running.
To avoid accidents, observe the rules of good laboratory practice while
handling solvents, reagents and consumables. Observe the safety
regulations about the used chemicals, as indicated in their specific
safety data sheets (MSDS). Check carefully the physical and chemical
1-4
properties of the substances before use.
Vapors from volatile solvents may be hazardous and flammable. Acidic
vapors may be corrosive for the mechanical parts. Be sure to respect
the safety measures defined by the national and international safety
rules in force.
The preparer can heat the reagent vials and the slides up to 95°C. For
that reason these zones are labelled as hot surfaces. Be careful to
avoid burns. Allow the reagents and slides to cool down before
handling them. Do not touch the reagent areas, the relative cover and
the reagents, barehanded or with the hands not adequately protected,
until they have cooled.
Do not obstruct in any way the air outlet opening at the rear of the
preparer. Keep an appropriate distance between the rear and side of
the machine and any other items that may obstruct the area in
question.
Periodically clean, with the machine off and cooled, the rear air outlet
opening.
When it is not used, place the cover of the slide area in the dedicated
compartment with the top down, not to partially obstruct the openings
for the draft of cooling air (see paragraph 5.2 “Installing and removing
the microFIND® area cover”).
Be sure to insert the waste tube into a suitable waste tank, and in any
case, dispose it properly.
In case of overflow of the liquid in the nozzle, disconnect the preparer
from the main power and decontaminate the area as indicated in
safety data sheets of the substances used, according to the national
and international safety regulations in force.
The device complies with part 15 of the FCC Rules. Operation is subject
to the following two conditions: (1) This device may not cause harmful
interference, and (2) this device must accept any interference received,
including interference that may cause undesired operation.
1-5
This equipment has been tested and found to comply with the limits for
a Class A digital device, pursuant to part 15 of the FCC Rules. These
limits are designed to provide reasonable protection against harmful
interference when the equipment is operated in a commercial
environment. This equipment can radiate radio frequency energy and,
if not installed and used in accordance with the instruction manual,
may cause harmful interference to radio communications. Operation of
this equipment in a residential area is likely to cause harmful
interference in which case the user will be required to correct the
interference at his own expense.
The preparer has been designed and tested according to CISPR 11
Class A regulation. In a domestic environment may cause radio
interference, in this case the user may need to take appropriate
measures to reduce the interference.
The preparer has been tested and it complies with the requirements
described in the standard IEC 61326, concerning the emission and
immunity relating to electromagnetic compatibility (EMC).
Do not use the preparer next to sources of strong electromagnetic
radiation (eg intentional RF sources unshielded), as they can interfere
with the proper functioning of the device. The level of electromagnetic
interference should be evaluated before using the product.
The warning signal stuck on the tower indicates the presence of
moving parts, including the syringe. Be careful to avoid bumps or cuts.
The biohazard warning stuck on the tower, near microFIND® and wash
nozzle area indicates the possible presence of hazardous substances.
This depends on the types of substances used with the preparer.
The warning signal stuck near the peristaltic pumps, wash nozzle and
tube connections indicates the possible presence of liquids, also
hazardous (this depends on the types of substances used with the
preparer). Be careful before to disconnect the tubes. In any case verify
that tubes are correctly connected before to use the preparer.
1.4 Manual Structure
Please read this manual before use to ensure correct operation of the autoFIND S
open.
Chapter 1
Introduction
1-6
Warnings and general characteristics.
Chapter 2
Equipment description
Part names, notes and control panel description.
Chapter 3
Installation
Preliminary operations, assembly, installation and switch off.
Chapter 4
Set up
Configuration and set up of the unit, Setup mode, installation
and syringe removal.
Chapter 5
Operations
Installation and removal of the vials and unit operation.
Chapter 6
Trouble shooting
Causes and solutions.
Chapter 7
Maintenance
Ordinary and extraordinary.
Chapter 8
Recycling and disposal
Appendix A
Consumables
Appendix B
Flow diagrams
Appendix C
Screen list
Appendix D
Interconnection panel
1.5 Applicable rules
Directive 2006/95/CE
Directive 2004/108/CE
Regarding low-voltage equipment
Regarding electromagnetic compatibility
1.6 General specifications
autoFIND S open is a preparative station for microFIND®.
On the preparative station 12 MicroFIND® can be placed.
The 12 MicroFIND® are placed in three different areas (each made of 4 locations) that
can be thermally conditioned at three different temperatures from 35°C to 90°C.
MicroFIND® can be incubated for a specific time with different reagent liquids that are
available in the station.
In fact in the station there are also:
1-7
•
•
•
6 not heated positions for reagent vials;
3 positions for reagent vials which can be heated from 35 to 90°C;
other 3 positions for reagent vials which can be heated from 35 to 90°C;
The machine is controlled by software installed on a PC via an RS 232 interface (USBRS232 adapters may be used).
It can:
•
•
•
•
create and calibrate the machine configuration;
create customized methods;
run the operations;
display the machine status during operation.
1-8
1.7 Technical specifications
MECHANICAL SPECIFICATIONS
DIMENSIONS
Height
Depth
Width
660 mm
466 mm
402 mm
WEIGHT
Standard
About 17kg
ELECTRICAL SPECIFICATIONS
AUTOFIND S OPEN
Power voltage
Frequency
Power
230 or 115 Vac ±10%
50/60 Hz
141 VA
INTERFACE SPECIFICATIONS
PC connection
RS-232 serial cable
ENVIRONMENT CONDITIONS
Room temperature limits:
Ambient humidity limits:
Maximum altitude:
15°C - 35°C
5% - 80% (not -condensable)
2000m
SOUND PRESSURE LEVEL
Maximum measured level
Negligible
SAFETY INFORMATION
HT127D Classification:
• Pollution degree 2
• Overvoltage category II
• Device for use indoors
HEATING SPECIFICATIONS
Temperature range programmable on
MicroFIND®
Temperature range programmable on heated
1-9
Off or from 35°C to 90°C
Off or from 35°C to 90°C
Reagent vials (areas 4B and 4C in Figure 1)
SOFTWARE
HTAPREP
Purpose built software for Microsoft Windows Operating
Systems
1-10
Chapter
2
2. EQUIPMENT
DESCRIPTION
2.1 Parts definition
3
6
4C
4A
4B
1
5
7
2
8
8
Figure 1 - System
1
2
3
4
TURRET
CONTROL PANEL
PERISTALTIC PUMPS
REAGENT VIAL AREAS
5
6
7
8
microFIND® AREAS
NOZZLE
TABLE
COVER
2-1
rotating turret, plunger and syringe
A: not heated reagent vials
B: heated reagent vials
C: heated reagent vials
for needle washing
for incubation of microFIND®
2.1.1
microFIND® area
The microFIND® devices must be placed in the locations shown in the figure
below (indicated with the number 5 in figure 1).
Figure 2 – microFIND® locations
The locations are 12. Each location is identified by a number from 1 to 12.
These locations are divided in three different areas (each made of 4 locations)
that are highlighted with three different colours in the figure. These areas can be
heated at three different temperatures from 35°C to 90°C.
2.1.2
Reagent vial areas
In the station there are 12 positions for reagent vials (indicated with the letter 4A4B and 4C in figure 1).
The 6 housings on the left (4A), not heated, are for:
Buffer B (blue label)
Buffer C (pink label)
Ethanol 70%
Ethanol 85%
Ethanol 100%
Carnoy’s fixative
The 6 housings on the right (4B and 4C) are thermally heated from 35°C to 90°C,
and are dedicated to the heating of the reagents; the housings in 4B are
predisposed for Buffer A (yellow label), Buffer P+H and Buffer D (green label), the
ones in 4C are available for the denaturation of the probes. autoFIND S open is
equipped with 3 metallic adaptors for 0,5ml vials.
2-2
Figure 3 – Reagent vial locations
2.1.3
Syringe location
The syringe is located inside the tower.
To access to the syringe remove the closing screw using the allen key supplied
with autoFIND S open and lift up the sliding lid.
2-3
Figure 4 – Sliding lid down
1
2
Sliding lid
Closing screw
2-4
Figure 5 – Sliding lid up
The syringe holder contains the following parts.
Figure 6 – Syringe not installed
Figure 7 – Syringe installed
Figure 8 – Syringe location detail (1)
Figure 9 - Syringe location detail (2)
2-5
1
2
3
Needle guide
Drawing nozzle
Syringe housing
4
5
Regulator lock
Plunger holder
6
Syringe holder
7
8
Plunger housing
Safety lock
without syringe in Figure 6
with syringe in Figure 7
for needle guide
inserted in Figure 8
not inserted in Figure 9
inserted in Figure 8
not inserted in Figure 9
for needle guide
The plunger locker has two different sides, for different plungers shapes. The two
faces are labelled “S” side and “B” side.
Figure 10 – Plunger locker: Side “S”
2-6
Figure 11 – Plunger locker: Side “B”
2.1.4
Connection panel
On the rear side of the autoFIND S open there is a connection panel, as described
in the following figure and table.
Figure 12 – AUTOFIND S OPEN connection panel
Power supply
1
2
Power setting
I/O
230 o 115 Vac
Power switch (ON/OFF)
Connectors
3
4
Power socket
RS232C
5
MULTI CONNECTOR
for power supply cable
for serial connection of AUTOFIND S OPEN with
PC
for connection to other instrumentation
2-7
2.1.5
Control panel
The control panel of the unit is laid out as follows.
Figure 13 – Control panel
Display
0
Alphanumeric VFD (Vacuum Florescence Device) type, 20 characters/2
lines. Shows command and status
Keys
1
2
3
4
5
6
7
8
9
10
11
SET
MET
SEQ
STOP
START
EXIT
ENTER
!
"
#
$
enters Setup mode
not enabled
not enabled
operative key
operative key
operative key
operative key
directional key (moves
up)
right)
down)
left)
N.B.: for further information on the keyboard, please refer to 2.3.
2-8
2.1.6
Wash nozzle
On the left of the AUTOFIND S OPEN there is the location of the nozzle for the
needle wash (indicated with number 6 in figure 1).
Figure 14 – Wash Nozzle
2.1.7
Cover
Under the control panel is inserted a cover for the microFIND® area, which allows
the incubation of microFIND® in controlled temperature and humidity conditions.
2.2 Moving parts
There are three moving parts:
•
the Plunger (named PLG), which can move maximum 64 mm inside the
syringe;
•
the Needle (named NDL), which lifts and lower the syringe and the needle
for maximum 210 mm from the higher position;
•
the Turret (named TUR), which can rotate from central position of about
±160°;
Warning
Each part may be moved carefully by hand, but only when the
instrument is switched off.
Rapid movements or movements with the autoFIND S open switched on can
damage it.
2-9
2.3 Control panel description
The control panel is used for manual control of some functions of the machine.
2.3.1
Typographic notes
For simplification each screen will be shown as follows:
line 1 %
line 2 &
Screen #
Normal characters (see “line 1”) indicate static lines (non changing).
Italic characters (see “line 2”) indicate dynamic (scrolling) lines.
Symbols % and & are a field that can have different values. These can be
changed when needed by using the keypad.
The screen# indicate the reference screen number.
Symbols !, ", # and $ will indicate keys the directional arrow keys on the right
hand side of the consol (for reference these are 8, 9, 10 and 11 in Figure 13).
Symbols SET , MET , SEQ , STOP , START , EXIT and ENTER indicate the
function keys on the left and at the bottom of the console (keys 1, 2, 3, 4, 5, 6
and 7 in Figure 13).
2.3.2
Directional keys
line 1 %
line 2 &
Screen #
The instrument display has two lines. If the line is active, it has a blinking cursor.
To move from one line to another:
•
press down ( #) to move to the next line;
•
press UP (!) to move to the previous line.
These keys (# and !) also move to another screen or menu:
•
from the upper line pressing ! moves to the previous screen;
•
from the lower line pressing # moves to the following screen.
The left and right arrow keys (" and $) modify the values shown in a specific line
or to move to a submenu.
The directional keys (#, !, ", $) are not always active in all screens.
The icon on the extreme right hand side of the screen displays which keys are
active.
2-10
Symbol
!
Active key
Symbol
Active key
!
"
"
$
#
#
More than one icon indicates that more than one key is active. If there is no icon
then in that screen the directional keys are not active.
A beep confirms the key has been pressed.
2.3.3
STOP, START, EXIT and ENTER keys
EXIT is a functional key. It is used to go back from a screen to the previous one
and to exit from a secondary menu and/or go back to the main menu.
STOP is used to stop the instrument when moving. The key toggles the status
from active (“RUN”) to hold (“STAND-BY”) or confirmation wait (“PAUSE”).
START is used to re-start an action after the STOP pressing.
ENTER is use to confirm an instruction to the instrument.
2.3.4
SET, MET and SEQ keys
SET enters the configuration menu (“SETUP” mode).
MET key is not enabled. It flashes when the microFINDs are cooled or warmed.
SEQ key is not enabled. It flashes when the reagent vials are warmed.
When the unit enters one of the SETUP, the respective key will be lit.
To exit the mode, the key will need to be pressed again. The exit is enabled only
from the first screen of the menu.
2-11
Chapter
3
3. INSTALLATION
3.1 Location
The machine must be positioned in a place that provides good ventilation. The
electronic circuits generate heat that is removed through an opening located in the
rear of the unit as shown in the figure below.
Figure 15 – Cooling opening
Do not obstruct in any way the air outlet opening at the rear of the preparer. Keep
an appropriate distance between the rear and side of the machine and any other
items that may obstruct the area in question.
The unit must be positioned on a level surface.
Environmental temperature must be from 15°C to 35°C and the relative humidity
must be between 5%- 85% (not condensing). If these environmental conditions are
not met, it may affect the performance or damage the unit.
3-1
3.2 Preliminary operations
Please take care during the unpacking procedure.
Check the integrity of the box to see if it has any external damage. If damage is
present please inform your local dealer before removing it from the box.
When the unit has been unpacked, check the machine and accessories provided
(compare these with the packing list enclosed).
Ensure that everything listed in the packing list is in the box. If not, please advise
your dealer.
3.3 Unit assembly
For the assembly of the parts it is necessary to perform the following actions
in this order:
1. Place the box on a table, open the box and remove the foam packing;
2. Remove the envelope containing the product sheet and the Declaration of CE
Compliance form;
3. Check the product paper to verify if the configuration is compliant with the
device and the accessories provided;
4. Extract the autoFIND S open from the box and place it on a flat surface. Remove
the protection cushions.
5. Place the machine in a vertical position, with the display facing forwards.
6. Remove the protection belt and polythene bag from the tower.
3.4 Installation
The system needs to be configured before use. This is done by the following
operations.
•
•
•
•
•
Ensure the voltage is set correctly (see paragraph 3.4.1).
Blocks removal (see paragraph 3.4.2).
Tubes positioning (see paragraph 3.4.3).
Install the syringe (see paragraph 4.5.1).
Finish the installation (see paragraph 4.2).
3-2
At the end of the installation, the unit should look similar to Figure 16.
Figure 16 – autoFIND S open assembled
3-3
3.4.1
Voltage control and set-up
Ensure that the AUTOFIND S OPEN is disconnected from the mains power.
On the rear side of the unit is the connection panel (see Figure 12).
The voltage is shown above the power switch. It is normally set to 230V.
To switch to 115V, perform the following operations:
Figure 17 – Opening fuses hollow
•
•
•
Figure 18 – Extracting fuses holder
Open the fuse compartment with a screwdriver, as shown in Figure 17.
With the screwdriver remove the fuse holder, as shown in Figure 18.
Substitute the 2 fuses, ensuring the correct rating is used (see Table 1
below).
Power voltage
115V
230V
Type (EN 60127)
3,15 A T
2AT
Table 1
•
•
•
Replace the fuse holder with the correct voltage on the top side.
Carefully close the fuse compartment.
Ensure the correct voltage can be read.
3-4
3.4.2
Blocks removal
•
•
Open the syringe holder. Remove the closing screw with the Allen key and
raise the sliding lid (see figure 4 and 5).
Regulate the security block of the needle-drive.
Warning
If the security block is not released and reset then as soon as the unit is
switched on it will give and error (most likely a touch sensor error).
Normally the regulation block is set to the correct length when the unit is
shipped – there is normally no requirement to change this block unless a
different type (length) of syringe is used.
The autoFIND S open is normally shipped in the transport position shown in Figure
19.
Figure 19 – Transport position
1
Regulation block
3-5
Figure 20 – Operating position
2
•
•
3.4.3
Security block
With the Allen key loosen the security block on the left needle-drive (see
Figure 19). The drive should be lowered about 30 mm, until the regulation
block of on the right (see Figure 20) rests on the ledge (see picture). Raise
the left block about 5 mm above the right block and tighten.
Carefully lift the needle-drive up and down by hand to make sure it doesn’t
stick or catch on the syringe sledge. If it does catch ensure that the blocks
are fixed pointing forwards and not to the side.
Tubes positioning
Figure 21 – Tubes positioning
•
•
3.4.4
Electrical connections
•
•
3.4.5
Put the waste tube into the waste tank.
Put the wash tube into the washing liquid tank.
Plug the power cable in the machine and then insert it in the main power.
Plug the second cable provided with the machine in the port identified as
RS232C to the serial port of the PC.
Start-Up
Turn on the power switch (“I” is on “O” is off). The unit will boot up and display
the following screen:
3-6
AUTOFIND S OPEN Series
Software rel. %
Screen 1
Field % defines the software version installed in the machine.
After 5 seconds the tower moves to the rest position and on the display shows:
Initializing
Please wait!
Screen 2
After the Start-up, the system will get to stand-by (ready to execute commands)
and displays the following.
STAND-BY
< WAITING COMMAND! >
Screen 3
3.5 Switch off
To turn off the power, use the power switch (position “O”), on the rear of the unit
(see Figure 12).
It is recommended that the unit is in Stand-By before doing this.
3-7
Chapter
4
4. SET UP
4.1 General
To configure the unit “Setup” mode must be used.
Warning
Set-up must be completed by competent personnel, properly trained about the
procedures described in this manual: any missing or different execution of the
described procedures can cause damages to the preparer or to the person.
However, in particular, pay attention to the moving parts as the sledge, syringe ,
needle etc...
Some parts of the preparer (reagent vials, slides) can work at high
temperatures. Be careful to avoid burns. Allow these parts to cool down before
handling them.
Wrong settings could cause damages to the syringe and/or to the preparer.
If some values in the fields are incomplete or incorrect, the SET led will remain
light even after you exit from the Set-up menu.
Warning
If you are using biological or hazardous substances, be sure to use the suitable
protective devices and to follow the appropriate precautions according to the
regulations in force for the safety in the laboratories.
4.2 Setup Mode
To enter the Setup mode the instrument must be in screen 1 (this is the first
screen after boot up that shows the version number). If the system is in screens 2
or 3, return to screen 1 by pressing EXIT . Alternatively turn on the power whilst
holding the SET key.
Screen 1 is shown for 5 seconds, before automatically moving to screens 2 and 3.
4-1
From screen 1, press SET to enter the Setup mode.
The SET key will light and the display shows:
Manual Operations
Syringe Setup
Screen 50
The above screen (50) is the initial screen of Setup mode.
Press # and ! to move between Manual Operation and Syringe Setup.
Manual Operations can be used to move each individual motor.
The Syringe Setup is used to install the syringe. To exit from Setup, press the
SET key again when in screen 50.
4.3 Manual Operations submenu
The submenu “Manual Operations” is used to individually move the plunger,
syringe sledge, tower, washing valve and aspiration valve.
At the first row of screen 50, press " to display the following:
PL NE TU WSH ASP TCH
% & '
Screen 0
Select one of the six functions using " and $.
Every function corresponds to an individual part of the unit:
•
PL: is the syringe plunger;
•
NE: is the syringe holder (syringe sledge);
•
TU: is the turret;
•
WSH: indicates the status of the washing valve.
•
ASP: indicates the status of the aspiration valve.
•
TCH: indicates the status of the touch sensor;
The selected command will flash, with ! and # keys it will be possible to move the
releted motor:
•
•
•
lift (!) or lower (# ) the syringe piston;
lift (!) or lower (#) the syringe holder;
rotate tower to the right hand side (!) or to the left hand side (#);
4-2
If the unit registers a movement error, “BS” will appear underneath the
corresponding letter (if Bs appears when moving but disappears when the motor
has stopped it is not a problem).
The symbol under the write WSH (field %) displays the status of peristaltic pump
that brings the washing liquid in the nozzle. It is possible to modify its spin speed
with keys ! and #. It starts from 25% of its maximum speed as Run default, and
with every ! or # pressing it is changed of 1%.
The field % displays:
Run
Wash peristaltic pump ON
---
Wash peristaltic pump OFF
If the wash peristaltic pump is activated automatically also the aspiration
peristaltic pump is activated to avoid that the liquid overflows from the nozzle.
The symbol under the write ASP (field &) displays the status of the aspiration
peristaltic pump that sucks the liquid dispensed by the needle in the microFIND
and finally moves it to the waste tank. It is possible to modify its spin speed with
keys ! and #. It starts from 25% of its maximum speed as Run default, and with
every ! or # pressing it is changed of 1%.
The field & displays:
Run
Aspiration peristaltic pump ON
Stop
Aspiration peristaltic pump OFF
The symbol under the write TCH (field ') indicates the state of the sensor touch.
‡
The needle-drive is touching surface.
=
The needle-drive is not touching a surface.
Return to screen 50, by pressing EXIT .
4-3
4.4 Submenu Syringe Setup
The Syringe Setup submenu is used to set the syringe parameters.
From the second row of screen 50, press " to access the submenu:
Syringe Size mL 500
Syringe Install
Screen 52
Field % defines the nominal volume of the syringe (this can be 250 or 500mL) and
can be selected using the left/right arrow keys.
Press down then the right arrow key to access the syringe installation mode (see
paragraph 4.5).
Press the down key twice to show the following screen:
Syringe Install
Touch & Plunger Zero
Screen 53
Press down and right to enter the acquisition mode - this is used to define:
•
the zero point of syringe plunger;
•
the touch position of the nozzle.
Touch & Plunger zero will run automatically when selected (press right). The
display shows:
ZERO PLUNGER
WAIT RUNNING
Screen 54
The syringe plunger moves down to the nozzle and automatically acquires the
zero point. It then finds the zero point of the nozzle, the display shows.
WASTE TOUCH
WAIT RUNNING
Screen 55
NOTE. If there is a problem with these functions, (or during syringe emptying)
then the unit will generate an error message.
After the routine has been completed the unit will move the plunger (PLG) so that
the base of the plunger aligns with the top of the syringe scale. If this is not
4-4
aligned properly ensure that the correct syringe is used and the correct volume is
entered into the unit.
If the error remains, contact Technical Support.
The unit then returns to screen 50.
4.5 Syringe installation
To install, to remove or to change the syringe, it is necessary to follow this
procedure.
4.5.1
First installation
•
•
•
•
•
•
•
•
•
•
•
•
Ensure that the unit is in stand-by mode.
Open the syringe location, remove the closing screw with the Allen key and
raise the sliding lid (see figure 4 and 5)
Enter Setup mode (see paragraph 4.2) and select the Syringe Setup (see
paragraph 4.4).
Ensure the Syringe Size matches the capacity of the syringe that is to be
installed.
Enter in the Syringe install mode by pressing down the right (# and ").
In screen 0 (see paragraph 4.3), move the plunger (PLG) to its lowest
position.
Move the syringe holder (NDL) about 5cm down from its highest position.
Loosen the regulation block (on the right), so that the needle guide drops
down and rests on the security block (on the left).
Remove the plunger lock with the screwdriver (see Figure 8 and Figure 9).
Remove the syringe block with the Allen key (see Figure 8 and Figure 9).
Raise the syringe holder (NDL) to its highest position.
Install the syringe holder of the syringe to be used (see Appendix A
CONSUMABLES).
There are two type of syringe holder, corresponding to two types of syringe.
Before the syringe installation, ensure that the correct syringe holder is
installed.
•
•
•
•
Ensure that the touch sensor (“1” in Figure 6) can be accessed.
Place the syringe in the unit and secure in place with the syringe holder.
Tighten the regulation block (on the right). The tip of the needle should be
perfectly aligned (level) with the needle guide. It must not be below the
needle guide as this may lead to needle damage.
The needle is aligned with the vial locators by placing the touch tool over the
waste so that it sits under the guide. When the needle begins to bend it is
aligned.
4-5
•
•
•
•
•
Release the security block (on the left) and tighten about 10 mm higher than
the regulation (right) block. Ensure it is fixed perpendicular to the syringe
holder (so that is doesn’t catch on the sledge runners).
Move the needle guide up and down by hand and ensure that it does not
catch. If it does then loosen and retighten the blocks ensuring they are
perpendicular with respect to the syringe holder.
Move up the plunger (PLG) and insert the plunger holder. Ensure it is
inserted in the correct position for the syringe type (see Appendix A
CONSUMABLES). The correct face is oriented down.
Raise the syringe holder (NDL) to its highest position.
Ensure that the needle of the syringe is correctly aligned, as shown in Figure
22.
YES
Figure 22 – Alignment needle-touch
•
•
•
•
Push EXIT to exit from screen 0. The unit reverts to screen 52.
Run the plunger zero routine (see paragraph 0).
Close the syringe location, by bringing down the lid and tightening with the
closing screw.
Exit from Setup mode by pressing SET from screen 50.
Warning
The security block on the left shaft must be fixed above the regulator block
on the right shaft; otherwise the unit will have a “touch sensor” error.
4.5.2
Removal
•
•
•
Ensure that the unit is in stand-by.
Open the syringe location, remove the closing screw with the Allen key and
raise the protective cover.
Enter Setup mode (see paragraph 4.2) and select “syringe Setup” with the
right key (").
4-6
•
•
•
•
•
•
•
4.5.3
Using the # and " Keys, select the syringe installation mode “Syringe
Install” (see paragraph 0).
In the screen 0 (see paragraph 4.3) move down the plunger (PLG) to its
lowest position.
Move the syringe sledge (NDL) to about 2 cm below its highest position.
Remove the plunger holder with the screwdriver (see Figure 8 and Figure 9).
Remove the syringe holder with the Allen key (see Figure 8 and Figure 9).
Carefully remove the syringe.
Replace the syringe holder, using the two screws.
Substitution
If the syringe to be installed is different from the previous syringe then use same
procedure as if it was the first installation (see paragraph 4.5.1).
If the syringe is being replaced by one that is identical in volume and dimension
then perform the following procedure.
•
•
•
•
•
•
•
4.5.4
Insert the new syringe, ensuring the top of the plunger fits into the plunger
holder (ensure the correct face of the plunger lock is used. This information
can be found in Appendix A CONSUMABLES).
Secure the syringe in place with the syringe holder clip.
Ensure that that the needle-drive moves freely. Move down the syringe
sledge (NDL) needle into the waste for a length of about 2-3 cm, ensuring
that the needle doesn’t bend. If the needle-drive doesn’t move correctly,
repeat the installation, following the instructions of paragraph 4.5.1, as if it is
a first installation.
Press EXIT to exit from the screen 0. Screen 52 should appear.
Run “Plunger zero” (see paragraph 0).
Close the syringe location, lowering the sliding lid cover and fixing the closing
screw.
Exit from Setup mode by pressing “SET” from screen 50.
Changing the Syringe holder
Before changing substituting the syringe holder, verify that:
•
the syringe holder is empty;
•
the syringe holder is mounted;
•
the plunger holder is mounted.
Perform the following procedure:
•
remove the screw from the centre of the syringe holder;
•
Extract the syringe holder;
•
Insert the new syringe holder, ensuring the intermediate needle guide is in
the same place;
•
Fix in place with the screw.
4-7
Chapter
5
5. OPERATIONS
Warning
These operations must be completed by competent personnel, properly trained
about the procedures described in this manual: any missing or different
execution of the described procedures can cause damages to the preparer or to
the person.
However, in particular, pay attention to the moving parts as the sledge, syringe ,
needle etc...
For the sample and reagents handling see paragraph 1.1 “Intended use and
restrictions” and 1.3 “Warning”
Warning
Some parts of the preparer (reagent vials, slides) can work at high temperatures.
Be careful to avoid burns. Allow these parts to cool down before handling them.
Warning
5.1 Installing and removing reagent vials
To install, remove or substitute the reagent vials (see 2.1.2), simply move the
turret out of the way (use screen 0, do not move the machine by hand when it is
switched on) then lift the vials.
Place with care the vials in their respective places (see 2.1.2).
5-1
5.2 Installing and removing the microFIND® area cover
When not used, the microFIND® area cover is located inside its dedicated
compartment, with the top down, as shown in the picture below.
Figure 23 – MicroFIND area cover positioning in its compartment
At the time of use, extract the cover from the compartment and wet the blotting
paper with demineralized water, in order to have the necessary humidity in the
microFIND® area during the incubation.
Place the cover over the microFIND® area, as shown in Figure 24
Figure 24 – microFIND® area cover installation
5.3 Running
The unit requires a PC with HTAPrep software to work (other than the technical
functions described previously).
5-2
When running, the unit displays action currently performed.
Movement of tower and needle
MOVE
RUNNING
Screen 4
Vial/MicroFIND presence
CHECK OBJECT
RUNNING
Screen 5
Aspiration from object
ASPIRE FROM OBJECT
RUNNING
Screen 6
Aspiration from pump
ASPIRE FROM PUMP
RUNNING
Screen 7
Needle washing
WASH NEEDLE
RUNNING
Screen 8
Dispensation
DISPENSE IN OBJECT
RUNNING
Screen 9
Unit’s stop
ABORT
RUNNING
5-3
Screen 10
5-4
Chapter
6
6. TROUBLESHOOTING
6.1 Preliminary
This chapter shows the error messages and some solutions.
If the solutions do not resolve the problems, please contact your local agent or
Technical Assistance.
Before contacting Technical Assistance:
•
ensure that all instructions are performed properly;
•
repeat the scheduled operations;
•
ensure that all cables are connected properly.
6.2 Sensor touch trouble
*Needle*
Check “Touch Sensor”!
Screen E24
Causes
•
The sensor touch doesn’t work correctly.
Proposed solutions
•
•
Press EXIT . The unit displays screen 0 of manual operations. This is likely
top becauses by the two vertical bars not fully coming down or (on
installation) having the safety lock lower than the touch sensor lock (see
chapter 4.3).
After the movements, push EXIT.
Running errors
6.2.1
Missing objects
CHECK OBJECT
NOT FOUND!
Screen E0
ASPIRE FROM OBJECT
NOT FOUND!
Screen E1
6-1
ASPIRE FROM PUMP
NOT FOUND!
Screen E2
WASH NEEDLE
NOT FOUND!
Screen E3
DISPENSE IN OBJECT
NOT FOUND!
Screen E4
ABORT
NO DETECTION!
Screen E5
ABORT
TOUCH SENSOR FAIL!
Screen E6
Causes
•
When the unit checks for the presence of an object during an operation, and
it can’t find it.
Proposed solutions
•
•
•
•
6.2.2
Insert the missing object (vial or microFIND)
Check the orientation of the microFIND.
Restart the operation.
If the object is in place run Autozero with all the devices positioned (vials
and microFIND) and retry. If on waste run “Plunger zero” in SET mode.
Obstacle
CHECK OBJECT
OBSTACLE FOUND!
Screen E7
ASPIRE FROM OBJECT
OBSTACLE FOUND!
Screen E8
6-2
ASPIRE FROM PUMP
OBSTACLE FOUND!
Screen E9
WASH NEEDLE
OBSTACLE FOUND!
Screen E10
DISPENSE IN OBJECT
OBSTACLE FOUND!
Screen E11
ABORT
OBSTACLE FOUND!
Screen E12
Causes
•
The unit finds an obstacle on some objects.
Proposed solutions
•
•
•
6.2.3
Remove the obstacle if present.
Restart operation.
If there is no obstacle then run autozero and retry. If on waste run “Plunger
zero” in SET mode.
Blocked movement
If the unit finds an obstacle during movement, the control system cuts off the
electrical power to the motors in 60 seconds and displays the following screen.
*WAIT*
Autocheck Running
Screen E13
During this screen, the system stores the positions of the motors, and then shows
screen 0.
The part of the unit that is blocked shows “Bs”.
% & '
Screen E14
6-3
% & '
Screen E15
% & '
Screen E16
Remove the obstacle, if present.
Use the " and $ keys to select the part of the unit to move, and the ! and # to
move the blocked motor.
Press EXIT to exit from the Manual Operations screen.
One of the following screen will appear if a task has been interrupted.
MOVE
MECHANICAL LOCK!
Screen E17
CHECK OBJECT
MECHANICAL LOCK!
Screen E18
ASPIRE FROM OBJECT
MECHANICAL LOCK!
Screen E19
ASPIRE FROM PUMP
MECHANICAL LOCK!
Screen E20
WASH NEEDLE
MECHANICAL LOCK!
Screen E21
DISPENSE IN OBJECT
MECHANICAL LOCK!
Screen E22
ABORT
MECHANICAL LOCK!
Screen E23
6-4
6.3 Syringe needle damage
The syringe lifetime will be related to:
•
the type of syringe and needle;
•
syringe installation and configuration.
If the syringe is often bending then:
•
Try and see how and in which position the damage is occurring.
•
Ensure that the alignment procedure on nozzle is correct.
•
Examine the vials to ensure they are correctly placed without caps.
•
Check that the syringe is correctly installed (see paragraph Syringe
installation 4.5).
•
Ensure that the needle-drive is correctly aligned with the tip of needle, as
shown in Figure 22.
•
Examine the nozzle to ensure it is not blocked.
6-5
Chapter
7
autoFIND S open Manuale d’uso
7. MAINTENANCE
Warning
These operations must be completed by competent personnel, properly trained
about the procedures described in this manual: any missing or different
execution of the described procedures can cause damages to the preparer or to
the person.
However, in particular, pay attention to the moving parts as the sledge, syringe,
needle etc...
restrictions” and 1.3 “Warning”
Warning
Some parts of the preparer (reagent vials, slides) can work at high temperatures.
Be careful to avoid burns. Allow these parts to cool down before handling them.
Warning
7.1 Ordinary maintenance
7-1
Ordinary maintenance of the unit includes:
•
•
•
7.1.1
Emptying and washing the nozzle;
Cleaning of the air outlet opening at the rear of the preparer;
External washing of the cabinet.
Washing procedure
To avoid the clogging of the tubes, a washing procedure is required. To this
purpose, a method named “WASH” is supplied. For details regarding loading and
running of the method, please refer to the HTAPREP software manual.
Execute the washing procedure at least once a week.
7.1.2
External cleaning of cabinet and air outlet opening
Proceed as described below:
•
switch off the device;
•
disconnect the power cable;
•
use a brush to remove the dust from the air outlet opening at the rear of the
preparer;
•
clean the control panel and other fragile parts with a wetted cloth;
•
clean the cabinet using a mild detergent solution;
•
wipe with a cloth.
Note: Never use solvents, detergents or spray on electrical parts.
7.1.3
Replacement of the blotting paper inside the cover
The blotting paper inside the cover of the microFIND® area must be replaced
periodically, in order to avoid the formation of mold.
To replace it, take the cover upside down and open the white clip at the center of
the cover. Remove the metallic grid from the cover and the blotting paper to be
replaced. Take a new blotting paper sheet (see Appendix A) and place it inside the
cover, put the metallic grid over it and close the clip.
7-2
Figure 25 – Replacement of the blotting paper
7.2 Extraordinary maintenance
This unit should not require any annual maintenance.
For any other maintenance operation not indicated in this manual, please contact
your Supplier or Technical Assistance Centre. The operator is not authorized to
perform any operation different from the ones of normal working.
7-3
Chapter
8
8. RECYCLING AND
DISPOSAL
Warning
These operations must be completed by competent personnel, properly trained about
the procedures described in this manual: any missing or different execution of the
described procedures can cause damages to the preparer or to the person.
restrictions” and 1.3 “Warning”.
Warning
Some parts of the preparer (reagent vials, slides) can work at high temperatures. Be
careful to avoid burns. Allow these parts to cool down before handling them.
Warning
8.1 Preparer uninstall
To uninstall the preparer perform the following operations in the given sequence:
1. Remove the syringe according to the instructions given in the dedicated
paragraph of this manual. Remove any residual substances from the instrument in
accordance with the applicable safety regulations;
2. Re-insert the syringe locker and the plunger locker;
8-4
3. Remove the reagent vials and the slides, if present, eliminating any residual
substances from the instrument in accordance with the applicable safety
regulations;
4. Raise the syringe lodging (NDL) to the top.;
5. Loosen the Safety lock (left);
6. Raise the needle guide until the shipping position (see figure 19 in this
manual);
7. Tighten the Safety lock (left);
8. Move down the sliding lid and tighten the closing screw;
9. Extract the wash tube from the washing liquid tank.
10. Switch on the WSH pump (status “RUN”, see paragraph 4.3 “Manual Operations
submenu). When the liquid stop going out from the wash nozzle (see paragraph
2.1.6 ”Wash nozzle”) switch off this pump (status ---). Empty the wash nozzle with
a laboratory syringe.
11. Switch on the ASP pump (status “RUN”, see 4.3 “Manual Operations submenu”).
When the liquid stop going out from the waste tube (see paragraph 3.4.3 “Tubes
positioning”) remove the waste tube from the waste tank and switch off this pump
(status “STOP”).
12. Switch off the preparer;
13. Unplug the power cord from the main power and then remove it from the
preparer;
14. Unplug the RS232 cable from the preparer port and from the serial port of the
PC.
8.2 Preparer transport
The preparer transport can be realized only in the original packaging. If the
preparer has already been installed you must do the following operations:
1. Execute the uninstall procedure described in paragraph 8.1;
2. Clean and treat to avoid biological, chemical and radiological risks;
3. Repack the various parts of the preparer in the original packaging;
4. Close the box by fixing the flaps using adhesive tape for packaging.
8.3 Preparer disuse
In case of preparer disuse or disposal, perform the following operation:
1. Execute the uninstall procedure described in paragraph 8.1;
8-5
2. Place the preparer and its accessory parts in the original packaging or into
another packaging suitable for transport; if you need further information, contact
your dealer or the Technical Assistance Centre.
8.4 Disposal instructions
HTA srl, as electronic devices manufacturer, is engaged in the financing and in the
management of electronic equipment's disposal activities. For updated information
about the disposal mode and the collection points:
WEEE: information about the recycling of Electronic and Electrical Equipment
European Union Countries
Registration Number to Italian EEE Registry: IT11110000007352
For information about the recycling of Electronic and Electrical Equipment get
in contact with us
Outside European Union
8-6
For information about the recycling of Electronic and Electrical Equipment get in
contact with us
8-7
Appendix
A
autoFIND S open User Manual
9. Appendix A
CONSUMABLES
Warning
restrictions” and 1.3”Warning”.
9.1 Syringes
The following table shows the syringe that can be used with the unit.
Other syringe can be used as long as they have the same properties as the one
listed below.
TETHIS
P/N
syr_aFS_50
0
Syringe
volume
Needle type
Plunger type
0.500 ml
Removable, 51mm,
G22
PTFE
The following table shows the removable needle to use.
TETHIS P/N
Length
Type
ndl_aFS_51
51 mm
G22
Point stile
LC
9.2 Reagent vials
TETHIS P/N
Description
rvl_aFS_2
12,5x49mm (2ml) vials with screw cap
9.3 Probe vials
TETHIS P/N
pvl_aFS_05
Description
0,5ml vials with cap
9-1
9.4 Cover blotting paper
TETHIS P/N
blp_aFS_
Description
Blotting paper sheets
9.5 Flasks
TETHIS P/N
Description
FLSK_aFS_250 250 ml plastic flask with holed cap
9.6 Manual syringe
TETHIS P/N
SYR/CUP
Description
Disposable syringe (5ml) with suction
cup end
9.7
9-2
Appendix
B
10.1
10. Appendix B FLOW
DIAGRAMS
Setup mode
10-1
Appendix
C
11.1
11. Appendix C SCREEN
LIST
Start-Up
AUTOFIND S OPEN Series
Software rel. %
Screen 1
Initializing
Please wait!
Screen 2
11.2
Run Time Menu
STAND-BY
WAITING COMMAND!
Screen 3
MOVE
RUNNING
Screen 4
CHECK OBJECT
RUNNING
Screen 5
ASPIRE FROM OBJECT
RUNNING
Screen 6
ASPIRE FROM PUMP
RUNNING
Screen 7
WASH NEEDLE
RUNNING
Screen 8
11-1
DISPENSE IN OBJECT
RUNNING
Screen 9
ABORT
RUNNING
Screen 10
11.3
Setup
% & '
Screen 0
Manual Operations
Syringe Setup
Screen 50
Syringe Size mL 500
Syringe Install
Screen 52
Syringe Install
Touch & Plunger Zero
Screen 53
ZERO PLUNGER
WAIT RUNNING
Screen 54
WASTE TOUCH
WAIT RUNNING
Screen 55
11-2
11.4
Errors
CHECK OBJECT
NOT FOUND!
Screen E0
ASPIRE FROM OBJECT
NOT FOUND!
Screen E1
ASPIRE FROM PUMP
NOT FOUND!
Screen E2
WASH NEEDLE
NOT FOUND!
Screen E3
DISPENSE IN OBJECT
NOT FOUND!
Screen E4
ABORT
NO DETECTION!
Screen E5
ABORT
TOUCH SENSOR FAIL!
Screen E6
CHECK OBJECT
OBSTACLE FOUND!
Screen E7
ASPIRE FROM OBJECT
OBSTACLE FOUND!
Screen E8
ASPIRE FROM PUMP
11-3
OBSTACLE FOUND!
Screen E9
WASH NEEDLE
OBSTACLE FOUND!
Screen E10
DISPENSE IN OBJECT
OBSTACLE FOUND!
Screen E11
ABORT
OBSTACLE FOUND!
Screen E12
*WAIT*
Autocheck Running
Screen E13
% & '
Screen E14
% & '
Screen E15
% & '
Screen E16
MOVE
MECHANICAL LOCK!
Screen E17
CHECK OBJECT
MECHANICAL LOCK!
Screen E18
11-4
ASPIRE FROM OBJECT
MECHANICAL LOCK!
Screen E19
ASPIRE FROM PUMP
MECHANICAL LOCK!
Screen E20
WASH NEEDLE
MECHANICAL LOCK!
Screen E21
DISPENSE IN OBJECT
MECHANICAL LOCK!
Screen E22
ABORT
MECHANICAL LOCK!
Screen E23
*Needle*
Check “Touch Sensor”!
Screen E24
11-5
Appendix
D
12. Appendix D
INTERCONNECTION PANEL
The connection panel is on the back of the unit.
Figure 26 – Connection panel
12.1
Output interface
The used connector is a female connector DB15.
DB15
pin #
1
Function
2
3
4
5
6
7
8
9
10
11
12
13
14
15
FREE3-IN
+5 Volts
SAMPINS-NO
GCRDY
Notes
Max. 20 mA
Input (active low): Level 0 = 0…2 V High Level = 4 .. 12 V
Relay contact normally open
Input (active high): Level 0 = 0…2 V High Level = 4 .. 12 V
GND
SAMPINS-COM
FREE1-NC
--GCRDY
FREE1-NO
--FREE2
FREE1-COM
--SAMPINS-NC
Common Relay contact
Relay contact normally closed
NOT AVAIBLE CONTACT
Relay contact normally open
NOT AVAIBLE CONTACT
Common Relay contact
NOT AVAIBLE CONTACT
Relay contact normally closed
Figure 27 – Connector I/O
I. AUTOFIND S OPEN_rev111206.doc
12-1
Relay Specifications: Vmax = 24 V ; Imax = 1A
Figure 28 – Typical interfacing
Figure 29 – Output Connector
12-2
12.2
RS232 interface
The RS232 connector is a female connector DB9.
DB9
pin #
1
2
3
4
5
6
7
8
9
Function
N.C.
TX
RX
DSR
Notes
not connected
Transmission
Receiving
Internally connected to DTR
DGND
Internally connected to DSR
DTR
CTS
RTS
N.C.
not connected
Figure 30 – RS232C Connector
12-3
FIGURE INDEX
Figure
Figure
Figure
Figure
Figure
Figure
Figure
Figure
Figure
Figure
Figure
Figure
Figure
Figure
Figure
Figure
Figure
Figure
Figure
Figure
Figure
Figure
Figure
Figure
Figure
Figure
Figure
Figure
Figure
Figure
1 - System ........................................................................................................ 2-1
2 – microFIND® locations ................................................................................... 2-2
3 – Reagent vial locations .................................................................................. 2-3
4 – Sliding lid down ........................................................................................... 2-4
5 – Sliding lid up ................................................................................................ 2-4
6 – Syringe not installed ..................................................................................... 2-5
7 – Syringe installed .......................................................................................... 2-5
8 – Syringe location detail (1) ............................................................................. 2-5
9 - Syringe location detail (2) ............................................................................. 2-5
10 – Plunger locker: Side “S” .............................................................................. 2-6
11 – Plunger locker: Side “B” .............................................................................. 2-6
12 – AUTOFIND S OPEN connection panel ........................................................... 2-7
13 – Control panel ............................................................................................. 2-8
14 – Wash Nozzle .............................................................................................. 2-9
15 – Cooling opening ......................................................................................... 3-1
16 – autoFIND S open assembled ....................................................................... 3-3
17 – Opening fuses hollow ................................................................................. 3-4
18 – Extracting fuses holder ............................................................................... 3-4
19 – Transport position ...................................................................................... 3-5
20 – Operating position ...................................................................................... 3-5
21 – Tubes positioning ....................................................................................... 3-6
22 – Alignment needle-touch .............................................................................. 4-6
23 – MicroFIND area cover positioning in its compartment .................................... 5-2
24 – microFIND® area cover installation .............................................................. 5-2
25 – Replacement of the blotting paper ............................................................... 7-3
26 - Connection panel…………………………………………………………………………………….12-1
27 - Connector I/O…………………………………………………………………………………………12-1
28 - Typical interfacing…………………………………………………………………………………..12-2
29 - Output Connector……………………………………………………………………………………12-2
30 - RS232C Connector…………………………………………………………………………………..12-3
I
SCREEN INDEX
Screen
Screen
Screen
Screen
Screen
Screen
Screen
Screen
Screen
Screen
Screen
Screen
Screen
Screen
Screen
Screen
Screen
Screen
Screen
Screen
Screen
Screen
Screen
Screen
Screen
Screen
Screen
Screen
Screen
Screen
Screen
Screen
Screen
Screen
Screen
Screen
Screen
Screen
Screen
Screen
00.................................................................................................................... 4-2
01.................................................................................................................... 3-7
02.................................................................................................................... 3-7
03.................................................................................................................... 3-7
04.................................................................................................................... 5-3
05.................................................................................................................... 5-4
06.................................................................................................................... 5-4
07.................................................................................................................... 5-4
08.................................................................................................................... 5-4
09.................................................................................................................... 5-4
18.................................................................................................................... 5-4
50.................................................................................................................... 4-2
52.................................................................................................................... 4-4
53.................................................................................................................... 4-4
54.................................................................................................................... 4-4
55.................................................................................................................... 4-4
E0 ................................................................................................................... 6-1
E08 .................................................................................................................. 6-2
E1 ................................................................................................................... 6-1
E10 .................................................................................................................. 6-3
E11 .................................................................................................................. 6-3
E12 .................................................................................................................. 6-3
E13 .................................................................................................................. 6-3
E14 .................................................................................................................. 6-3
E15 .................................................................................................................. 6-4
E16 .................................................................................................................. 6-4
E17 .................................................................................................................. 6-4
E19 .................................................................................................................. 6-4
E2 ................................................................................................................... 6-2
E20 .................................................................................................................. 6-4
E21 .................................................................................................................. 6-4
E22 .................................................................................................................. 6-4
E23 .................................................................................................................. 6-4
E24 .................................................................................................................. 6-1
E3 ................................................................................................................... 6-2
E5 ................................................................................................................... 6-2
E6 ................................................................................................................... 6-2
E7 ................................................................................................................... 6-2
E8 ................................................................................................................... 6-2
E9 ................................................................................................................... 6-3
II

AUTOFIND S OPEN rev111206 low-res

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