Elektronische neuzen in de (intensieve) veehouderij
Transcription
Elektronische neuzen in de (intensieve) veehouderij
Elektronische neuzen in de (intensieve) veehouderij Ervaringen en een literatuur onderzoek. Auteur: Drs Ries MJM Kock voorzitter Stichting Leefbaar Buitengebied Gelderland Inhoud: 1. 2. 3. 4. 5. Inleiding De geurwet van 2007 De evaluatie E-nose systeem E-nose systeem voor gebruik in de (intensieve) veehouderij a. Onderzoek uit Canada b. EMS 6. Conclusies en aanbevelingen 1 Inleiding De leden van de burgergroeperingen zijn ervaringsdeskundigen als het gaat om stank uit (intensieve) veehouderijen. Al jaren ageren zij tegen de aanwezigheid, de uitbreiding of de komst van (intensieve) veehouderijen. Daarbij is vrijwel altijd de stank de directe aanleiding om zich te organiseren en te verdiepen in de oorzaken. Wij zien dat bij een buurtgroepering het standpunt “not in my backyard” al snel verandert in “not in anybodies backyard”. Waarom zou een bewoner op het platteland in de stank moeten wonen? Nog verdere verdieping leidt er toe dat de groeperingen tot de conclusie komen dat de (intensieve) veehouderij in Nederland uit de hand gelopen is. De overtuiging is groeiende dat niet schaalvergroting de (intensieve) veehouderij duurzaam zal maken, maar de kringloop van productie sluiten een voorwaarde is. Of, als voorbeeld, geen mestvergisters en mestproducten naar de wijngebieden, maar in stand houden van bossen in Zuid Amerika, minder vlees eten en produceren in Europa en afbouw van niet-gesloten-kringloop-landbouw in Europa. Onlangs kwam in het nieuws dat 20% van de intensieve varkenshouderijen in Nederland technisch failliet is. De bedrijven zijn de facto door de banken overgenomen maar lijden voortdurend verlies. Volgens De Telegraaf formuleerde de staatssecretaris haar standpunt nadrukkelijker dan de NVV en LTO het weergeven. De krant citeert haar als volgt: „Er wordt gewoon te veel varkensvlees geproduceerd, niet alleen in Nederland", zegt ze. Dat probleem speelde volgens haar al voor de Russische boycot. „Het wegvallen van de Russische markt heeft het probleem alleen maar versneld", stelt de PvdA-bewindsvrouw. Zij wijst er bovendien op dat er ook problemen zijn in landen die varkenshouders geen extra kosten opleggen. „We produceren gewoon te veel", herhaalt ze. De sector wil hetzelfde aantal dieren of zelfs meer produceren, ook al verdient ze er niet aan. De staatssecretaris wil productie toestaan, zolang we eraan verdienen. Maar waar blijft het besef bij de sector en de staatssecretaris dat we duurzaam moeten produceren? Dat de voedselproductie zodanig dient te zijn dat de gehele mensheid nu en in de toekomst gezond voedsel ter beschikking heeft? Zoals onlangs is geformuleerd door het VN High Level Panel: 1. Uitbanning van armoede 2. Verbetering van de positie van vrouwen en meisjes en het bereiken van gelijkheid van sekse 3. Kwalitatief onderwijs 4. Gezonde levens 5. Toegang tot voedzaam eten en voedselzekerheid 6. Universele toegang tot water en sanitaire voorzieningen 7. Verzekerde en duurzame energie 8. Groei van banen en welzijn 9. Duurzaam gebruik van natuurlijke hulpbronnen 10. Goed bestuur en effectieve instituties 11. Creëren van stabiele en vreedzame samenlevingen 12. Een eerlijk mondiaal systeem met lange termijn financieringsmogelijkheden De bestrijding van stank uit (intensieve) veehouderij zal in verband gebracht worden met omvattende problematiek van “toegang tot voedzaam eten en voedselzekerheid”. 2 De geurwet van 2007 Aan de orde is de evaluatie van de geurwet van 2007. Deze is onder meer bedoeld om omwonenden van (intensieve) veehouderijen te beschermen tegen geurhinder. Deze wet faalt in die opzet om een aantal redenen: - - - De zogenaamde grens van 14 odeur garandeert stankoverlast. Buren van intensieve veehouderijen klagen al jaren over stankoverlast en worden geconfronteerd met goedgekeurde vergunningen. De praktijk leert dat de wet niet werkt. De grens van 14 odeur beschermt weliswaar het ondernemerschap van de ondernemer, maar niet zijn welzijn en zeker niet die van zijn buren. Bij de buren gaat de vlag uit wanneer een (intensieve) veehouderij stopt of failliet gaat. De wet schrijft een berekening voor (V-Stacks) die toegepast wordt bij het al dan niet toekennen van een vergunning van een uitbreiding of nieuwbouw. Slechts een gedeelte van de stank die een bedrijf voortbrengt wordt aan de berekening onderworpen. Zo wordt de stank bij het overpompen van mest, het roeren in mest, het vullen van silo’s, het maken van brijvoer, het in werking hebben van mestvijvers, het indikken van mest niet meegenomen in de berekening. De maximale 14 odeur uit de berekening blijkt in de praktijk vaak veel hoger. De cumulatie van stank van meerdere bedrijven op een bewoner wordt niet meegenomen in de geurwet. De 50% regeling. De wet blijkt niet te handhaven zo is de ervaring van buren. Wanneer een bedrijf onder gelijke omstandigheden op het ene moment (bijvoorbeeld in het weekend) buitengewoon meer stinkt dan op het andere, dan komt de controlerende instantie niet onmiddellijk vaststellen wat er aan de hand is, maar vele dagen later, wanneer de stank zich niet meer voordoet. De geurwet 2007 heeft tot gevolg dat een ondernemer kan zeggen dat hij/zij aan de wet voldoet, terwijl buren klagen over overmatige stank. Beiden hebben gelijk. De gevolgen ervan zijn: - Een ondernemer die voldoet aan de wet wordt desondanks gezien als een buur die het woongenot van zijn buren ernstig benadeelt. Dat naast de geuroverlast en de gevolgen voor de gezondheid ook andere emissies van (intensieve) veehouderijen nadelig zijn voor de gezondheid (voorbeeld: Q-koorts) Daling van waarde van onroerend goed van buren. Er zijn voorbeelden bekend van een daling van de WOZ waarde van 35% bij een afstand van 70 meter; en 20% bij 250 meter. Taxaties bevestigen dat. Doordat buren gaan klagen en de ondernemer daarvoor geen reden ziet gaat de cohesie in buurten achteruit. Verjaardagen worden niet samen meer gevierd, kinderen spelen niet meer samen en een enkele keer leidt het tot woordenwisselingen en zelfs bedreigingen. De geurwet van 2007 heeft een haat creërende werking. 3 De evaluatie In de evaluatie spelen twee onderzoeken een belangrijke rol. Dat van PRA Odournet uit 2006 en het recente onderzoek van GGD/IRAS. Beide onderzoeken hebben verschillende uitkomsten. Er is een groot verschil in de gerapporteerde geurbelasting. Die valt bij GGD/IRAS veel hoger uit dan bij PRA Odournet. Het RIVM stelt op grond van een vergelijking van de onderzoeken vast: De rekenmodellen voor geurbelasting zijn verouderd, waardoor de berekende geurbelasting kan afwijken van de praktijk. Huidige stallen hebben meerdere uitlaatpunten met warmte inhoud; de emissies variëren (afhankelijk van groei van de dieren, verschil tussen dag en nacht); er zijn veranderingen in stalontwerp, huisvesting, ventilatie en voeding. Al deze factoren kunnen niet genegeerd worden. Het verdient aanbeveling rekening te houden met verschillen in perceptie (plaats, tijd en persoon) die kunnen leiden tot andere hinderpercentages bij gelijkblijvende blootstelling. En daarmee ook tot een grotere of minder grote mate van geurhinder. Het hanteren van een algemene modellen is daarom niet in alle situaties verstandig. De burgergroeperingen, vertegenwoordigd in de werkgroep max5odeur, hebben er een hard hoofd in dat er ooit nog eens modellen komen die vrij nauwkeurig de werkelijke geurbelasting kunnen voorspellen. Op meerdere terreinen is in de agrarische sector sprake van een kloof tussen de gemodelleerde werkelijkheid en de feitelijke, gemeten werkelijkheid. Daarbij komt dat modellen voor de leek ondoorgrondelijk zijn en fraude bij toepassing niet kan worden uitgesloten. Om die reden zijn burgergroeperingen groot voorstander van geurmetingen. Bij de vergunningverlening zou meer waarde moeten worden toegekend aan metingen dan aan berekeningen. Dat kan door vergunningen af te geven onder voorwaarde dat met behulp van sensoren objectief is vastgesteld dat omwonenden geen stankoverlast ondervinden. Wanneer daarna toch stankoverlast wordt gerapporteerd, kunnen er via handhaving aanvullende maatregelen afgedwongen worden. Er is internationaal onderzoek gaande naar de mogelijkheden om geur uit veehouderijen elektronisch te meten. We behandelen hier Canadees onderzoek uit 2011 en de ontwikkeling van een geursensor op basis van Multi Gas Sensor Array (MGA) voor het gebruik van geurmetingen bij varkensstallen van het Nederlandse bedrijf EMS. 4 E-nose systeem Een oplossing van de problemen van de gebreken van de geurwet van 2007 boven genoemd is een systeem dat - 24 uur per dag 7 dagen in de week de stank objectief kan meten 24 uur per dag 7 dagen in de week, de ondernemer, zijn / haar buren en de controlerende instantie actief informeert informatie geeft over de aard van de stank stimuleert dat (intensieve) veehouderijen verder bij buren vandaan komen te staan De kern van zo’n systeem is een elektronische neus. Deze bestaat uit een rij van elektronische sensoren, die (delen van de) geur meten en die via elektronica en software wordt omgezet in kennis van de geur. An electronic nose (e-nose) is a device that identifies the specific components of an odor and analyzes its chemical makeup to identify it. An electronic nose consists of a mechanism for chemical detection, such as an array of electronic sensors, and a mechanism for pattern recognition, such as a neural network . Electronic noses have been around for several years but have typically been large and expensive. Current research is focused on making the devices smaller, less expensive, and more sensitive. The smallest version, a nose-on-a-chip is a single computer chip containing both the sensors and the processing components. An odor is composed of molecules, each of which has a specific size and shape. Each of these molecules has a correspondingly sized and shaped receptor in the human nose. When a specific receptor receives a molecule, it sends a signal to the brain and the brain identifies the smell associated with that particular molecule. Electronic noses based on the biological model work in a similar manner, albeit substituting sensors for the receptors, and transmitting the signal to a program for processing, rather than to the brain. Electronic noses are one example of a growing research area called biomimetics , or biomimicry, which involves human-made applications patterned on natural phenomena. Electronic noses were originally used for quality control applications in the food, beverage and cosmetics industries. Current applications include detection of odors specific to diseases for medical diagnosis, and detection of pollutants and gas leaks for environmental protection. Bron: http://whatis.techtarget.com/definition/electronic-nose-e-nose Samengevat: - een elektronische neus is een apparaat dat een aantal componenten van een geur identificeert en de sterkte ervan vaststelt. dat gebeurt via een reeks sensoren, die de aanwezigheid van een chemische geurstof meetbaar maakt een computer bewerkt de gegevens en geeft als resultaat een gemeten geurwaarde af 5 E-nose systeem voor gebruik in de intensieve veehouderij Twee publicaties zijn in dit verband relevant. 1. Remote Monitoring and Analyzing Livestock Farm Odour Using Wireless Electronic Noses door Leilei Pan The University of Guelph , Ontario, Canada © Leilei Pan, November, 2011 2. Rapportage ontwikkeling geur sensor voor het gebruik van geurmetingen bij varkensstallen. Electronic determination of odour by olfactory with the Multi Gas Analyser (MGA) Interpretation by an Artificial Neural Network Opmerking: Er zijn veel meer publicaties die facetten van e-noses bespreken. Deze twee springen eruit omdat ze betrekking hebben op e-noses in (intensieve) veehouderijen. Beide publicaties zijn als bijlage toegevoegd. Remote Monitoring and Analyzing Livestock Farm Odour Using Wireless Electronic Noses Array met sensoren Array met sensoren met luchtpomp 6 E-nose met: Processor (computer), data verwerving en preproces bord, voeding, carbon filter, sensoren kamer en luchtpomp. Conclusies in dit rapport: A portable electronic nose with 14 gas sensors, a humidity sensor and a temperature sensor was successfully developed especially for objective measurement of livestock farm odours. Experiments were conducted in field downwind from 14 livestock and poultry farms. Experimental results demonstrate that “Odour Expert” is a useful tool in supporting livestock and poultry farm odour management, which greatly improves the efficiency of livestock farm odour management and thus benefit livestock industry. The electronic nose is capable of producing accurate output with greater consistency in odour measurement. It is easier and cheaper to operate than using olfactometry or human panel. Thus it has good commercial potential. However, an electronic nose can only evaluate odour events on one point; it cannot provide an overall odour mapping around livestock facilities that is necessary for an overall odour management strategy. It is suggested that an electronic nose network combined with an air dispersion model is possible to predict the downwind odour strengths on the basis of odour emission rates, topography and meteorological data. Therefore, it can locate odour strength around livestock facilities in real time, and help the development of an overall odour management strategy. De conclusies bevestigen dat een elektronische neus met 14 gas sensoren en een temperatuur sensor in staat is om objectief de geur van een kippenstal te meten. Daarmee kan met succes geurmanagement worden bedreven. De elektronische neus is in staat om voldoende precies te meten. Het is goedkoper dan een menselijk panel. Het kan de sterkte van geur rondom een stal meten in real time. 7 Het bedrijf EMS B.V., Raiffeisenstraat 24, 4697 CG SINT-ANNALAND zegt op zijn website: Geur sensor op basis van Multi Gas Sensor Array (MGA) voor het gebruik van geurmetingen bij varkensstallen en ten behoeve van geuroverlast bepalingen. De Elektronische neus Geur is een samenstelling van moleculaire stofdeeltjes in lucht. Om geuren te ruiken heeft een mens ongeveer 30 miljoen reukcellen. Deze cellen reageren op de stofdeeltjes in lucht waardoor de hersenen de geur kunnen analyseren. Dit heet het reukvermogen. Het reukvermogen is het meest gevoelige zintuig van de mens. Het is het enige zintuig dat direct met het emotionele brein in werking staat. Hierdoor wordt het handelen van de mens direct gemanipuleerd door de omgevingslucht. Omdat dit niet in alle situaties gewenst is kan er ook worden gesproken over geuroverlast. Geuroverlast wordt veroorzaakt door een te hoge concentratie stofdeeltjes in lucht waardoor de fysieke of geestelijke gezondheid van de mens in gevaar wordt gebracht. Het vakgebied wat de geurbeleving bestudeert heet olfactometrie. Olfactometrie is een zeer complex gebied omdat zowel met objectieve als subjectieve waarnemingen wordt gewerkt. In de intensieve veehouderij is geuroverlast een groot probleem omdat een grote concentratie geur bronnen (varkens) aanwezig is in een dicht bevolkt gebied. Om geuroverlast te bepalen worden geurmetingen gedaan waarbij een groep mensen de geurende lucht gaan beoordelen. Deze methode is tijdrovend, duur en niet reproduceerbaar. Omdat er geen goede alternatieven op de markt zijn om eenvoudig geurmetingen uit te voeren bij varkensstallen heeft EMS gekeken naar de haalbaarheid van een elektronische geurmeting. EMS heeft een geurmeetsysteem ontwikkeld dat gebruikt kan worden voor metingen van geuroverlast bij een varkensstal. Het elektronisch bepalen van geuroverlast. De geuroverlast wordt in 3 stappen bepaald: 1. De sensor (Multi Gas Analyzer) bepaald een “vingerafdruk” van de geurende lucht. Het bepalen van hiervan is zo geoptimaliseerd dat het relatief weinig tijd inneemt. Deze vingerafdruk wordt vervolgens ingevoerd in een kunstmatig neuraal netwerk. 2. Het kunstmatig neuraal netwerk is een computer algoritme wat aan de hand van de vingerafdruk de concentratie van de verschillende geurcomponenten kan bepalen. 3. De bepaalde concentraties worden omgezet naar European Odour Units (OUe). Dit is een eenheid voor geurconcentratie vastgelegd in de NEN-13725 en VDI 3882. Vervolgens wordt hieruit de geurbeleving bepaald. Aan de hand van deze geurbeleving is het mogelijk om te bepalen of er sprake is van geuroverlast. 8 Bij navraag ontvingen wij de volgende aanvullende informatie: EMS: ‘’Bijgevoegd een application note met de beschrijving van een project dat we hebben uitgevoerd onder SBIR. Het systeem is momenteel niet commercieel te verkrijgen. (Dat zegt niet dat we dit niet willen en kunnen, maar we hebben op dit moment geen "standaard artikelnr.") Het hier beschreven systeem is bedoeld als prototype. Inmiddels hebben we 2 van deze systemen gebouwd.’’ EMS is bereid om een proefopstelling te laten zien. ‘’Evt. kan een bezoek aan ons lab erg waardevol zijn. We kunnen in ons lab elke geur maken die men wenst. Inmiddels zijn we aan een systeem bezig waarmee we een geur uit 24 gascomponenten kunnen laten bestaan. Dit vormt een extreem dichte benadering van de daadwerkelijke geur. Met deze opstelling leren we de geursensor ook in.’’ EMS is tevens bereid om een presentatie te geven over dit onderwerp voor de werkgroep evaluatie Wet geurhinder veehouderij. Doel is dan om over "geurmeten" meer algemene en basisinformatie te verstrekken. Bijgevoegd nog 2 foto's, 1 foto is de MGA gemonteerd bij een gaswasser van een varkensstal. De 2e foto is een foto van een geurmeetsysteem voor de bepaling van geur van vis. (Project dit jaar uitgevoerd). Het gaat bij EMS dus om echte kwalitatieve en kwantitatieve bepaling van de meer complexe geurprofielen. Daarnaast ontwerpen, bouwen en leveren wij voor de agrarische markt meetsystemen met lage detectiegrenzen voor allerhande toepassingen. Denk aan bewaring van producten, zoals fruit groente, bloembollen, maar ook permanente meting van (schadelijke) sprorengassen tijdens reguliere teelt in de glastuinbouw. Opmerkelijk is dat de hedonische waarde (de mate waarin een geur vies gevonden wordt) een rol speelt bij uitkomst van de verwerking van de meting. In de bijlage meer informatie over het project van EMS. De ‘’Rapportage ontwikkeling geur sensor voor het gebruik van geurmetingen bij varkensstallen’’ sluit af met: ‘’De praktische uitvoering en het gebruik van deze sensor is onder handbereik, maar hangt af van de vraag van de overheden en de handhavende instanties.’’ 9 Conclusies: - E-nose systemen zijn in ontwikkeling Eén Nederlandse ondernemer geeft aan dat e-nose-systeem gereed is om getest te worden. Hij wil de werkgroep evaluatie WGV en de Burgergroeperingen nader informeren. Onderzoeksvragen Omdat de huidige methodiek die gebruikt wordt in de geurwet van2007 zoveel te wensen overlaat heeft met spoed ontwikkelen van een e-nose-systeem een hoge prioriteit. Wij formuleren de volgende onderzoeksvragen. Is er een e-nose systeem dat voldoet aan de volgende criteria: - - Het systeem is in staat om real time de stank van een (intensieve) veehouderij te meten. Het systeem kan zo ingesteld worden dat het consistent onderscheid kan maken tussen de volgende vijf situaties o Er is een geur maar die is niet waarneembaar voor mensen o Er is een geur maar die is maar door de helft van de mensen waarneembaar (zeer mild) o Er is een geur maar die wordt niet als stank ervaren door 4 van de 5 mensen o Er is een geur die wordt door 4 van de 5 panel mensen als stank ervaren o Er is een geur die wordt door 4 van de 5 panel mensen als ernstige stank ervaren Het systeem is in staat om in 4 van de 5 gevallen een oorzaak van de stank aan te wijzen Het systeem is in staat om bij de aanwezigheid van meer dan één stal, van elke stal vast te stellen: o Er is een geur maar die is niet waarneembaar voor mensen o Er is een geur maar die is maar door de helft van de mensen waarneembaar (zeer mild) o Er is een geur maar die wordt niet als stank ervaren door 4 van de 5 mensen o Er is een geur en die wordt door 4 van de 5 mensen als stank ervaren o Er is een geur en die wordt door 4 van de 5 mensen als ernstige stank ervaren Wanneer de vraag bevestigend wordt beantwoord is de volgende vraag? o o Is het systeem te bouwen voor een prijs lager dan € 10.000 en onderhoudskosten lager dan € 1000, per jaar, per (intensieve) veehouderij. Op welke termijn zijn de systemen bij een afname van 1000 stuks te leveren? Bijlagen 2. 10 Remote Monitoring and Analyzing Livestock Farm Odour Using Wireless Electronic Noses by Leilei Pan A Thesis presented to The University of Guelph In partial fulfilment of requirements for the degree of Doctor of Philosophy in Engineering Guelph, Ontario, Canada © Leilei Pan, November, 2011 ABSTRACT REMOTE MONITORING AND ANALYZING LIVESTOCK FARM ODOUR USING WIRELESS ELECTRONIC NOSES Leilei Pan Advisor: University of Guelph, 2011 Professor Simon X. Yang A wireless electronic nose network system has been developed for monitoring and analyzing livestock farm odour. The system utilizes electronic noses (e-noses) that can measure odour compounds and environment factors such as temperature and humidity. The e-noses are deployed at various locations on the farm, and sensor signals are transmitted via a wireless communication to a central station, where the data processing and sensor fusion algorithms analyze the collected odour data, compute the odour concentration, and display the odour dispersion plume. This system would provide users with convenient odour monitoring capabilities and help the development of an effective overall odour management strategy. In addition, an adaptive neuro-fuzzy inference approach is proposed to calibrate the e-nose responses to human panelists’ perception. The proposed method can handle non-numeric information and human expert knowledge in livestock farm odour models, and can adjust the parameters in a systematic manner for optimal system performance. The proposed approach has been tested against a livestock farm odour database. Several livestock farm odour models have been developed for comparative studies. The results show that the proposed approach provides a more accurate odour prediction than a typical multi-layer feedforward neural network. Furthermore, to model odour dispersion around livestock facilities, a biologically inspired odour dispersion model is proposed, and is tested using computer simulations and a livestock farm odour database. Results show that the proposed approach is effective in providing accurate modelling of odour dispersion from multiple and various types of odour sources in both static and non-static environments. Dedication To my loving and understanding parents and to my dear husband. iii Acknowledgements Fist of all, I would like to acknowledge the contributions of the many people who provided invaluable help and support for the completion of this thesis. I would like to thank my advisor, Dr. Simon X. Yang, for his invaluable guidance, assistance, encouragement, and support during the course of this research and through my graduate studies at the University of Guelph. Special thanks as well to my advisory committee member Dr. Gauri Mittal, Dr. Staphano Gregori, and Dr. Fangju Wang, who has provided me precious advice and suggestions. The School of Engineering has provided a supportive environment in which to study. I would like to take this opportunity to express my sincere appreciation to all the faculty, staff, fellow students, and my friends. On a personal front, I am forever indebted to my family for my continued study. Special thanks to my parents and my husband, who have provided both moral and financial support over the period of my education. Without all of their love and support, I wouldn’t have been able to finish this thesis. There have been many people who have actively contributed to this project. All of their help and support is greatly appreciated. iv Contents List of Tables viii List of Figures ix List of Symbols xi List of Abbreviations xiii 1 Introduction 1 1.1 Problem Statement . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2 1.2 Objectives of This Thesis . . . . . . . . . . . . . . . . . . . . . . . . . 4 1.3 Contributions of This Thesis . . . . . . . . . . . . . . . . . . . . . . . 5 1.4 Organization of This Thesis . . . . . . . . . . . . . . . . . . . . . . . 7 2 Background and Literature Survey 2.1 2.2 2.3 9 Odour Measurement Technologies . . . . . . . . . . . . . . . . . . . . 9 2.1.1 Human Olfactory Based Methods . . . . . . . . . . . . . . . . 9 2.1.2 Non-Olfactory Odour Measurement Methods . . . . . . . . . . 11 Livestock Farm Odour Calibration Modelling . . . . . . . . . . . . . 14 2.2.1 Single Component Calibration Models . . . . . . . . . . . . . 14 2.2.2 Multi-Component Calibration Models . . . . . . . . . . . . . . 15 2.2.3 Multi-Component Multi-Factor Calibration Models . . . . . . 15 Odour Calibration Algorithms . . . . . . . . . . . . . . . . . . . . . . 16 2.3.1 16 Statistical Methods . . . . . . . . . . . . . . . . . . . . . . . . v 2.4 2.3.2 Neural Network Based Methods . . . . . . . . . . . . . . . . . 18 2.3.3 Fuzzy Logic Based Methods . . . . . . . . . . . . . . . . . . . 20 2.3.4 Other Methods . . . . . . . . . . . . . . . . . . . . . . . . . . 21 Air Dispersion Modelling . . . . . . . . . . . . . . . . . . . . . . . . 22 2.4.1 Fluid Dynamics Models . . . . . . . . . . . . . . . . . . . . . 22 2.4.2 Gaussian Dispersion Models . . . . . . . . . . . . . . . . . . . 24 2.4.3 Lagrangian Particle Models . . . . . . . . . . . . . . . . . . . 29 3 A Wireless Electronic Nose Network for Monitoring Livestock Farm Odour 30 3.1 Introduction . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 30 3.2 The Developed Wireless Electronic Nose Network System . . . . . . . 32 3.2.1 Development of the Electronic Noses . . . . . . . . . . . . . . 33 3.2.2 The Wireless Electronic Nose Network . . . . . . . . . . . . . 36 3.2.3 Software Integration . . . . . . . . . . . . . . . . . . . . . . . 39 3.3 Discussion . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 47 3.4 Summary . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 49 4 A Neuron-fuzzy Model for Odour Calibration 50 4.1 Introduction . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 50 4.2 The Proposed Neuro-fuzzy Approach . . . . . . . . . . . . . . . . . . 52 4.3 Experimental Results . . . . . . . . . . . . . . . . . . . . . . . . . . . 55 4.3.1 Data Sets . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 56 4.3.2 Livestock Odour Calibration Models . . . . . . . . . . . . . . 56 4.3.3 Results . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 59 Summary . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 60 4.4 5 A Neuron-dynamics Model for Odour Dispersion 64 5.1 Introduction . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 64 5.2 The Proposed Neuro-dynamics Approach . . . . . . . . . . . . . . . . 66 5.3 Stability Analysis of the Proposed Dispersion Model . . . . . . . . . . 68 vi 5.4 Simulation Results . . . . . . . . . . . . . . . . . . . . . . . . . . . . 69 5.4.1 Odour Dispersion in Static Environments . . . . . . . . . . . . 69 5.4.2 Odour Dispersion in Non-static Environments . . . . . . . . . 71 5.5 Experimental Results . . . . . . . . . . . . . . . . . . . . . . . . . . . 75 5.6 Discussion . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 77 5.6.1 Comparison to Gaussian Models . . . . . . . . . . . . . . . . . 77 5.6.2 Comparison to Fluid Dynamics Models . . . . . . . . . . . . . 81 5.6.3 Comparison to Lagrangian Particle Models . . . . . . . . . . . 82 Summary . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 83 5.7 6 Conclusions and Future Work 84 6.1 Conclusions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 84 6.2 Future Work . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 85 References 87 Appendix: Selected Publications 101 vii List of Tables 2.1 Pasquill-Gifford air stability classes . . . . . . . . . . . . . . . . . . . 26 3.1 Sensors used in the development of the electronic nose 34 viii . . . . . . . . List of Figures 2.1 A multi-component multi-factor model for livestock farm odour. . . . 17 3.1 The architecture of the proposed wireless electronic nose network. . . 33 3.2 The developed sensor array. . . . . . . . . . . . . . . . . . . . . . . . 35 3.3 The developed chamber. . . . . . . . . . . . . . . . . . . . . . . . . . 36 3.4 An electronic nose consists of the sensor array inside a chamber, a data acquisition board, a mote processor, and a power supply. . . . . . . . 37 3.5 The block diagram of the wireless network. . . . . . . . . . . . . . . . 37 3.6 The mote processor MPR2400 and the gateway MIB600. . . . . . . . 38 3.7 The gateway with connection to a laptop computer. . . . . . . . . . . 39 3.8 Topology of the wireless network. . . . . . . . . . . . . . . . . . . . . 42 3.9 The interface of data acquisition system. . . . . . . . . . . . . . . . . 42 3.10 The interface of database. . . . . . . . . . . . . . . . . . . . . . . . . 43 3.11 The interface of data records management console. . . . . . . . . . . 44 3.12 The main interface of the software. . . . . . . . . . . . . . . . . . . . 45 3.13 The dynamic display console. . . . . . . . . . . . . . . . . . . . . . . 46 3.14 The odour analysis system. . . . . . . . . . . . . . . . . . . . . . . . . 47 4.1 The structure of an ANFIS consists of a five-layer feedforward network. 53 4.2 An ANFIS model with three inputs and 27 IF-THEN rules for calibrating the livestock farm odour. . . . . . . . . . . . . . . . . . . . . 58 4.3 Structure of NN model for calibrating the livestock farm odour. . . . 58 4.4 Comparison of the initial membership functions before the learning (A) with the final membership functions after learning (B) using Model 1. ix 61 4.5 Comparison of the initial membership functions before the learning (A) with the final membership functions after learning (B) using Model 2. 62 5.1 Odour dispersion from a point source simulated by the proposed model. 70 5.2 Gaussian distribution test on the crosswind distributions generated by the proposed shunting model. . . . . . . . . . . . . . . . . . . . . . . 72 5.3 Odour dispersion from a line source simulated by the proposed model. 73 5.4 Odour dispersion from a regular area source simulated by the proposed model. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5.5 Odour dispersion from an irregular area source simulated by the proposed model. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5.6 75 Odour dispersion in changing wind speed simulated by the proposed model. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5.8 74 Odour dispersion from an instantaneous emission simulated by the proposed model. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5.7 73 76 Comparison of odour dispersion from a point source using the proposed model and a typical Gaussian dispersion model. . . . . . . . . . . . . x 78 List of Symbols api The Sugeno parameter associated with the i-th input and the p-th output in a fuzzy rule A The passive decay rate of neural activity Aij The j-th linguistic label associated with the i-th input B The upper bound of the neural activity c̄ (x, y, z) Steady-state concentration of pollutant at location (x, y, z) at a specific unit (g/m3 ) C Concentration of the pollutant C(Xi , t) The odour concentration at location Xi and time t dij The Euclidean distance between positions Xi and Xj D The lower bounds of the neural activity fp The p-th consequent output of the a rule H Effective source height i, j, k Indexes of a neuron in the neural network Ii+ The excitatory inputs to the i-th neuron Ii− The inhibitory inputs to the i-th neuron Kx Diffusion coefficient at horizontal downwind direction Ky Diffusion coefficient at horizontal crosswind direction Kz Diffusion coefficient at vertical direction m Number of input membership functions of a fuzzy rule MDP The mass consumption term that represents the deposition and biochemical interaction effects MSC The source term that represents the mass flow-in xi n Number of inputs of a fuzzy rule Opk The p-th output of the k-th layer of a ANFIS model Q The source strength or emission rate of the pollutant (g/s) r Correlation of the predicted value and perceived value u(θ) The angle function of wind direction (θ) Vw (t) The wind speed at time t Vx Horizontal downwind wind velocity (m/s) Vy Horizontal crosswind wind velocity (m/s) Vz Vertical wind velocity (m/s) wij The connection weight from the j-th neuron to the i-th neuron wp The firing strength of the p-th rule x Downwind distance to the odour source (m) xi The i-th input linguistic variable in the antecedent part of a fuzzy rule Xi The spatial location of the i-th neuron y Crosswind distance to the central line of the odour source (m) z Vertical distance to the central line of the odour source (m) μAij Membership function of the linguistic label Aij θ The angle between the wind direction and the position of the neighbour neuron ξi The neural activity of the i-th neuron σy Lateral Gaussian dispersion coefficient at crosswind direction σz Vertical Gaussian dispersion coefficient xii List of Abbreviations A/D Analog/Digital ANFIS Adaptive network-based fuzzy inference system ANN Artificial neural network BP Back propagation CA Cluster analysis CNN Competitive neural network DC Direct current DT Dilution to threshold DFA Discriminant function analysis FCNN Fuzzy clustering neural network FD Fluid dynamics FE Finite element FV Finite volume GA Genetic Algorithm GC/MS Gas chromatography/Mass Spectrometry GRNN Generalized regression neural network MCE-nose Multi-chamber electronic nose MLP Multi-layer perceptron MOS Metal-oxide semiconductor MSE Mean squared error NN neural network NN1 neural network with all factors and components NN2 neural network without considering ammonia xiii NN3 neural network without considering hydrogen sulfide PCA Principal components analysis PLS Partial least squares RBF Radial basis function RMSE Root mean squared error SLF Structural learning with forgetting VOC Volatile organic compound xiv Chapter 1 Introduction Despite its significant contributions to the Ontario economy, the livestock industry is now facing an increasing number of public complaints and concerns from non-farming rural residents about environmental and health problems caused by odour emissions from livestock operations. It is recognized that long-term exposure to nuisance odour usually results in undesirable reactions in people, including emotional stresses, respiratory problems, headaches, nausea, and vomiting (Sucker et al., 2009; Kartavtsev et al., 2011; Sakawi et al., 2011). The Environmental Protection Act has addressed the issue of odours that create health problems. In order to reduce the impact of odour emissions from livestock facilities, governments have devoted significant effort to limiting the expansion and development of livestock operations; as a result, the growth of the livestock industry has been impeded by its odour problems. The development of odour control technologies is progressing. However, evaluating the effectiveness of existing odour control technologies and methods is a difficult task because there is no convenient and reliable method for quantifying livestock farm odour strength. The lack of livestock farm odour measurement tools stems from the complexity of livestock farm odour and a limited understanding of the odour itself. In addition, the lack of convenient odour measurement tools prevents governments and environmental agencies from establishing regulations or standards to mitigate livestock farm odour problems. 1 1.1 Problem Statement Odour is a sensation caused by odorous chemicals in the air. Odour strength perceived by the human nose is a result of combined processes of physiological detection of stimuli by the olfactory receptors in the nose and psychological processing of the stimuli by the human brain (Kammermeier et al., 2001; Masaoka et al., 2007). People’s sensitivity to odour is very personal, and varies with gender, age and state of health. Therefore, it is difficult to achieve an objective and analytical measure of odour. Over 100 odorants have been identified in livestock farm odours. In the human nose, each odorant is detected by a combination of different olfactory receptors, and responses from all receptors are combined in neurons in the olfactory cortex in the brain. It is reported that the responses of cortical neurons are not just a combination of responses to the individual odorants of an odour, but actually generate novel odour perceptions in humans (Zou and Buck, 2006). Therefore, the strength of an odorant mixture is not a simple sum of the strength of all odorous compounds within it. This character of the human olfactory perception adds difficulty to odour measurement. The following problems affect research on livestock farm odours: 1. The human nose is still the “instrument” for odour measurement most commonly used today. Odour measurement technologies such as olfactometry and scentometer use trained odour panelists to smell air samples to determine the strength of an odour. Those human olfactory-based methods are susceptible to the analyst’s personal bias in odour measurement. It is difficult to overcome an individual’s bias regarding the influence of various factors - such as age and health, and personal background and experience - on the human sense of smell (Schiffman et al., 1995; Sucker et al., 2009; Sakawi et al., 2011). In addition, use of a human panel is expensive. Therefore, the need exists for unbiased automatic odour evaluating technologies. Electronic noses are objective, automatic, and low-cost odour evaluating devices that can detect and recognize complex odours. However, odour sources 2 in a modern livestock facilities usually are arrays of points (exhaust fans), lines (side curtain opening) and areas (manure spreadings). The odour profile is affected by farmstead factors such as building layout schemes, separation distances between buildings, and location of exhaust fans. Odour measurements at a single location, therefore, is not sufficient to provide a comprehensive odour impact evaluation around the farm; measurements taken at multiple locations simultaneously are required for an overall odour profile. A single electronic nose (enose) can assess odour levels at only one location at a time; it cannot provide a comprehensive odour impact evaluation based on various topography and farmstead factors, a fact that has led to an unfocused and inefficient research approach on reducing livestock farm odour. Therefore, an odour measurement technology that can provide a comprehensive odour profile around livestock facilities is required in the development of an overall odour management strategy. 2. Efforts to reduce odours from livestock production facilities have been impeded by the lack of knowledge on odour generation mechanisms. Livestock farm odours result from complex mixtures of hundreds of compounds that interact with each other; and the strength of an odour is not a simply the sum of the strengths of all odorous compounds within it (Zhang et al., 2003). Besides, many factors such as air temperature, air velocity, and relative humidity influence people’s perception of odour. However, how and to what extent these factors and components influence odour concentration is still unclear (Powers and Bastyr, 2004; Schauberger et al., 2005; Lin et al., 2007). 3. Detecting and locating odour around livestock farms has long been an elusive goal of researchers and environmental agencies. Air dispersion models are used as mathematical tools to calculate the downwind odour strength based on odour emission rates, topography and meteorological information. Researchers have identified important aspects in which agricultural odour sources are different from industrial pollutant sources (Zhu, 1998; Xing, 2006). For instance, odour sources in livestock farms are low-lying - usually at or near ground level; and the 3 rise of a livestock odour plume is not obvious because the vertical momentum or lower density of a mass flow of warm gas is not significant. Researchers have also indicated that some industrial-oriented air dispersion models are not suitable for agricultural odour dispersion (Xing, 2006). These differences have to be taken into account when calculating air dispersion. In addition, most existing air dispersion models require prior knowledge about the environment such as emission rate of gas at the source, incoming solar radiation, and mixing height - for example, the distance between the Earth’s surface and the bottom of the inversion atmospheric layer. These parameters are difficult to obtain without access to special professional tools and expertise, thus preventing a convenient estimation of the impact of livestock farm odour on the environment. Because odour emissions from livestock farms have unique characteristics, an odour dispersion model designed specifically for livestock farms can greatly improve the efficiency and accuracy of odour dispersion prediction. However, none of the existing dispersion models are specifically developed for livestock farm odour. 1.2 Objectives of This Thesis The objectives of this research are: (1) to develop an electronic nose network system for monitoring and analyzing livestock farm odours; (2) to develop an artificial intelligent model for analyzing and exploring odour generation; and (3) to propose a neuro-dynamics model for dynamic simulation of odour dispersion around livestock farms. The wireless network based system can provide objective, comprehensive and remote measurement for odours around livestock farms. Together with the artificial intelligence models, this electronic nose system can provide farmers and researchers with more precise odour management capabilities for more efficient operation of odour control systems. When combined with odour calibration and dispersion analysis approaches, the system can help in the development of an overall odour management strategy by providing dynamic, comprehensive information about the livestock farm environment and odour dispersion. 4 1.3 Contributions of This Thesis This work is different from other odour-related work in the following ways: 1. In this study, a wireless network system is developed for monitoring and analyzing livestock farm odour. The system utilizes a wireless network built from electronic noses that can detect odour compounds and environment factors such as temperature, wind speed, and humidity. The electronic noses are located at various points on the farm, and sensor signals are delivered via wireless link to an analysis station, where sensor fusion algorithms and odour dispersion algorithms calculate the odour concentration and dispersion; display the odour data in real time; and, should any environment factor change, predict the odour concentration. The proposed electronic nose network approach can automatically monitor livestock farm odours, but also has potential industry applications. It differs from existing electronic noses - which can measure at only a single location - in that it provides multi-location odour monitoring. The proposed wireless electronic nose network, therefore, can provide a more comprehensive odour profile than a single electronic nose. Second, by using this wireless electronic nose network system, users can monitor the odours from a remote location such as inside a house or office, as opposed to working in the field, making odour measurement easier in unfavourable weather. In addition, the developed electronic nose network system with multiple electronic noses is quite robust to sensor failure and noise. One or more failed or incorrect sensor readings can be corrected through the redundant information from the other electronic noses. 2. A multi-component, multi-factor odour calibration model is proposed to adequately model livestock farm odour. Both numeric factors and linguistic factors are considered in this model. The proposed multi-component multi-factor odour calibration model and neuro-fuzzy based approach can incorporate more potential odour generation factors into the model. Many non-numeric factors and 5 expert knowledge that cannot be incorporated into previous statistical models or neural network models can be considered by this approach. Thus it enables a more adequate and comprehensive odour calibration modelling 3. A neuro-fuzzy based method is proposed for analysis of the livestock farm odour. The proposed approach incorporates neuro-adaptive learning techniques into a fuzzy logic method. In addition, instead of choosing by trial and error the parameters associated with a given membership function, the parameters are tuned automatically in a systematic manner so as to adjust the membership functions of the input/output variables for optimal system performance. This feature is important as it can avoid inappropriate predetermination of membership functions, thus minimizing errors resulting from the limited knowledge on the livestock farm odour. 4. Air dispersion models have been used to predict the concentration of pollutants from industrial sources. Dispersion models use source emission rates and meteorological data to calculate the downwind concentration of chemical compounds as a function of the distance to the source. However, odour concentration is different from the concentration of chemical compounds, as it is a result of the combined processes of physical dilution of chemical compounds and biological olfactory sensing. Therefore, existing dispersion models are not very suitable for simulating odour dispersion. A novel neuro-dynamics approach is proposed to model dynamic odour dispersion around livestock farms. The dynamics of each neuron is characterized by a shunting equation derived from Hodgkin and Huxleys’s membrane equation (Grossberg, 1982). The proposed dispersion model can dynamically represent complex or non-steady-state environmental features around livestock farms. The model can also model odour dispersions from multiple odour sources, and from various types of sources like point sources, line sources, and area sources. The proposed neuro-dynamics odour dispersion model is developed specifically for odours around livestock farms. In this model, odour plume is represented by 6 the landscape of the neural activity for all neurons in the topologically organized neural network, and the dynamics of each neuron is characterized by a shunting neural equation. The dispersion parameters are therefore tuned according to input/output data to represent the nonlinear relationship among inputs and outputs. The proposed model is capable of simulating air dispersion both in static and non-static environments, and is able to model emissions from various types of odour sources, such as ventilation hoses, manure pits, and manure spreadings in fields. It is therefore more suitable than existing dispersion models for representing odour dispersion around livestock farms. 1.4 Organization of This Thesis This thesis consists of six chapters. Chapter 1 provides an introduction to livestock farm odour problems and outlines the objectives and contributions of the research. Chapter 2 summarizes the background and reviews the state-of-the-art research on odour measurement technologies, odour calibration algorithms, and odour dispersion modelling methods. Chapter 3 briefly presents the current status of odour measurement research, our previous work on development of an electronic nose for measuring livestock farm odour, and problems existing in current odour measurement technologies. Based on this review, a wireless electronic nose network is proposed for providing remote, automatic and objective odour measurements at multiple locations around livestock facilities. The architecture of the wireless network system and the design and development of compact electronic nose nodes and wireless communication links are discussed in detail. The development of a data management software system is described for supporting users in the processing and analysis of livestock farm odour data collected by the wireless electronic nose network. The structure and design of the software system is also presented. Chapter 4 reviews the limitations of current odour calibration models and algorithms. A multi-component, multi-factor model is proposed for better representing 7 livestock farm odour, and an adaptive network-based fuzzy inference system (ANFIS) is proposed to overcome limitations of existing odour calibration algorithms. The proposed approach is tested with a livestock farm odour database. Various comparative studies have been performed and the proposed method demonstrates the effectiveness of the proposed multi-component, multi-factor model and ANFIS method. Chapter 5 reviews existing air dispersion models and their limitations in modelling odour dispersion around livestock facilities. A novel neural network model is proposed for modelling dynamic odour dispersion around livestock farms. The development of the proposed model is explained in detail, and the stability and sensitivity analysis of the model are discussed. Odour dispersions in various farmstead (point, line, and area odour source) situations, and in static and non-static environments, are investigated by computer simulation. Preliminary testing with field livestock farm odour data is described, showing the effectiveness of the proposed neural network-based method in modelling odour dispersion around livestock farms. Chapter 6 summarizes, and draws conclusions from, the study on monitoring and analyzing livestock farm odour using the developed wireless electronic nose network, and the proposed odour calibration method and odour dispersion model. Recommendations for future work based on the findings of this research are presented. 8 Chapter 2 Background and Literature Survey Developing a system for monitoring and analyzing livestock farm odours requires technical knowledge in various areas. These areas can be categorized into odour measurement technologies, livestock farm odour calibration modelling, odour calibration algorithms, and air dispersion modelling. This chapter provides background knowledge and reviews existing research in these areas. 2.1 Odour Measurement Technologies A number of technologies are available for measuring odour strength around livestock facilities, such as dynamic dilution olfactometry and scentometer, which takes advantage of the human sensory response, and automatic electronic methods. 2.1.1 Human Olfactory Based Methods Currently trained human panelists are most commonly engaged in odour measurement technologies. Human olfactory based technologies include dynamic-dilution olfactometry for laboratory environment and scentometer for field measurement. 9 Dynamic-Dilution Olfactometry Dynamic-dilution olfactometry is widely used as a standard method of odour measurement (ASTM, 1986; Brose et al., 2001; McGinley and McGinley, 2004; Schmidt and Cain, 2009; Monse et al., 2010; Liu et al., 2010). When using dynamic-dilution olfactometry, air samples are presented to trained odour panelists over a series of dilution ratios through a sniffing port. Panelists smell the continuously diluted air samples, and evaluate the odour strength based on odour detection threshold. Odour concentration is then established as the dilution factor at the detection threshold. Several types of dynamic-dilution olfactometry are currently available. They vary from single port olfactometry to three ports olfactometry, and range from single panelist unit to multi-panelist units. When using single port olfactometry, panelists only evaluates the diluted air from the single sniffing port, while when using three ports olfactometry, three air samples are presented through the ports, two of which are odour-free air and one is diluted odour. The panelist must identify the diluted odour from then three samples. Only one panelist at a time can measure odour when using single panelist dynamic-dilution olfactometry, but multi-panelist can simultaneously evaluate odour through multi-panelist olfactometry. However, dynamic-dilution olfactometry has to be conducted in strictly controlled laboratory environment, where gas and dust concentrations are significantly different than in natural, open space. Thus, this method is not suitable for sampling odour downwind from odour sources in the field. Moreover, odour measurement using dynamic-dilution olfactometry requires a sufficient number of panelists. Panelist selecting and training procedures are time-consuming and of high cost. Scentometers Similar to dynamic-dilution olfactometry, scentometers is a hand-held dilution device that are also used to measures odour strength based on odour threshold. But in comparison to dynamic-dilution olfactometry, a scentometer is specifically intended for field studies. It dilutes odorous ambient air with carbon-filtered odourless air, 10 and odour concentration is established in “Dilutions-to-Threshold” (D/T) values. The Dilution-to-threshold is the dilution ratio of volume of carbon-filtered air to the volume of odorous air at the odour threshold (Kosmider and Krajewska, 2007; Bereznicki et al., 2010). A scentometer is portable, simple to use, relatively of low cost, and can be used to provide real-time odour measurement in the field. However, it has limited dilution levels; therefore it is less accurate than dynamic olfactometry. Because human sensory is involved, both dynamic-dilution olfactometry and scentometers are susceptible to the panelists’ personal bias in odour measurement. Many factors such as age and health, and individual’s background and experience, would influence the human sense of smell (Schiffman et al., 1995; Sucker et al., 2009; Sakawi et al., 2011). It is difficult to overcome this personal bias in odour measurement. In addition, human panelists are expensive to use. Therefore, unbiased automatic odour evaluating technologies are desirable. 2.1.2 Non-Olfactory Odour Measurement Methods Non-olfactory odour measurement technologies in are summarized in this section. The advantages and weaknesses of each technology are discussed. Gas Chromatography/Mass Spectroscopy Analysis Gas Chromatography/Mass Spectrometry analysis (GC/MS) is an analytical technology to identify and quantify of volatile organic chemical compounds within a sample gas. Its applications include drug detection (Hans et al., 2007), food and beverage quality control (Petrova et al., 2007), environment analysis (Eckenrode, 2001), and identifying chemical components in livestock odours (Schiffman et al., 2001a; Koziel et al., 2010). GC/MS analysis is conducted in strict laboratory environment, gas compounds should be maintained at certain vapour pressures during analysis. Before the gas sample is introduced into the GC/MS instrument, sample preparation and cleanup is required, e.g. the sample gases need to be dissolved in a suitable solvent. Formal training and expertise in mass spectrometry and chemistry is required for the 11 operation of the instrument. In addition, the analysis procedure typically takes 20 to 100 minutes, and additional data analysis can take more time depending on the amount of information required (McMaster, 2008). Generally, GC/MS can be used for identification and quantization of chemical compounds in livestock farm odours, but it doesn’t directly measure the strength of odour. Additional data analysis procedure is in need to transform the quantity of chemical compounds into odour strength. Moreover, it should be performed in laboratory, it is not suitable for field analysis; the analysis procedure is relatively slow, a GC/MS instrument is very expensive compared to other analytical systems and operational cost is high. Electronic Noses An electronic nose is a sensor-based electrical odour analysis system that is used for measurement of both quality and quantity of complex odours. Electronic nose is built to mimic human olfactory system, and usually consists of an array of electronic gas sensors (as counterpart of olfactory receptors in human nose) and a signal-processing system (mimics the part of human brain that processes odour stimuli) (Brattoli et al., 2011). During a measurement, odorous gases are introduced into the electronic nose, flow through the gas sensors. Gas sensors respond to the odour, and produce electrical signal outputs. The signal-processing system analyzes sensor signals, and transforms digital signals into meaningful odour quality/quantity results. The output of an electronic nose can be the identity and amount of chemical components in the odour, a prediction of odour strength, or any other characteristic properties of the odour which might be perceived by a human. Many applications of electronic noses have been reported. The most common application is to classify the smell of beverages or foods, in order to measure the freshness for quality control (Zhang et al., 2008). Electronic nose technology also has proved to be a useful tool in medicine for diagnosing (Kodogiannis et al., 2008), and in environmental monitoring for analyzing fuel mixtures (Sobaski et al., 2006), testing food and water quality (Devarajan et al., 2011), and identifying household 12 odours (Al-Bastaki, 2009). Gonzalez-Jimenez et al. (2011) proposed a multi-chamber electronic nose (MCE-nose) to overcome a major disadvantage of using metal oxide semiconductor (MOS) sensors for electronic noses, e.g. the long recovery period needed after each gas exposure. The proposed MCE-nose comprises several chambers, and in each chamber there is an identical sets of MOS sensors. Chambers and sensors alternate between sensing and recovering states, thus provide rapid sensing ability for the electronic nose. However, the number of studies dedicated to using an electronic nose in agricultural environment management is still very limited (Osarenren, 2002; Powers and Bastyr, 2004). No commercially available electronic noses were developed in particular for detecting livestock farm odour. Moreover, most electronic noses developed previously are bulky, desk-top instruments that can only be used in laboratories. The environments of laboratories are apparently different from those of the fields. Thus, the accuracy and reliability of the measurements are limited. It is highly desirable to develop new electronic nose for the measurement livestock farm odour which has great commercial potential. In my previous study, an intelligent portable electronic nose was developed particularly for measurement and analysis of livestock farm odour, and can be used in both laboratory and field studies (Pan and Yang, 2007). Experiments in 14 Ontario livestock farms showed that the electronic nose is capable of producing an objective output, has a greater consistency in odour measurement, and is easier to operate than using olfactometry or human panel. Electronic noses are unbiased, automatic, and low-cost odour evaluating devices which can detect and recognize complex odours. However, a modern livestock facility is usually not a point source but rather an array of points (exhaust fans), lines (side curtain opening) and areas (outdoor storage surfaces). The odour profile is affected by building layout schemes, separation distances between buildings, locations of exhaust fans, and landscape around livestock facilities. An electronic nose can only evaluate odour events at one location each time; it cannot provide a comprehensive odour impact evaluation according to topography, meteorological factors, and farm13 stead factors such as building layout schemes, which has led to an unfocused and inefficient research approach on reducing livestock farm odour. Therefore, an odour measurement technology that can provide an overall odour profile around livestock facilities is desirable for developing an overall odour management strategy. 2.2 Livestock Farm Odour Calibration Modelling The outputs from automated odour measurement instruments such as GC/MS and electronic nose are in terms of concentration of chemical compounds or electrical voltages or currents. They need to be correlated with human olfactory perception, and transformed into odour strength units based on odour calibration models. Odour strength is relative personal and subjective matter. It is subject to human olfactory sensitivity and human brain’s response to stimuli. Limited understanding of odour system and human olfactory and brain system has impeded the progress of odour calibration modelling. Researchers have indicated livestock farm odours are complex mixtures of hundreds of compounds that interact with each other; and the concentration of an odour is not a simply sum of concentrations of all odorous compounds within it (Zhang et al., 2003). Besides, many factors such as air temperature, air flow speed, and relative humidity (O’Neill and Philips, 1992; Schiffman et al., 2001b; Zahn et al., 2001) influence people’s perception of odour. 2.2.1 Single Component Calibration Models Researchers initially attempted to identify a single chemical compound as an odour indicator. Hydrogen sulfide and ammonia have been used as such indicators (Liu et al., 1993; Guo et al., 2000). Later research found that this single-component analysis method is incomplete, and a more thorough multi-component analysis is required (DiSpirito et al., 1999; Janes et al., 2004). 14 2.2.2 Multi-Component Calibration Models Some researchers have investigated multi-component calibration approach. Schiffman et al. (2001a) demonstrated there are hundreds of chemical components in livestock farm odour, and odour strengths are resulted from aggregate effect of hundreds odorous chemicals. Many chemicals at lower concentration actually produce higher odour strengths than other chemicals at higher concentration do. Therefore multicomponent analysis must be performed when correlate odorous chemicals with odour strengths. Zahn et al. (2001) conducted direct multiple-component analysis of malodorous volatile organic compounds (VOCs) present in ambient air from 29 livestock production facilities using a 19-component statistical model. This study provided evidence that a direct multi-component analysis of VOCs may be useful in monitoring livestock odours, however, it did not show that multi-component analysis of odours is more accurate than single-component analysis. 2.2.3 Multi-Component Multi-Factor Calibration Models Many researchers have indicated that the multi-component calibration approach should be extended to include odour generation factors, such as temperature, wind speed, relative humidity, solar radiation, etc. (Powers and Bastyr, 2004; Janes et al., 2005). Factors that probably influence livestock odour also include type of livestock facilities, number of animals, location of odour source, and many other relevant factors (Janes et al., 2005; Miller et al., 2004; Lin et al., 2009). Zahn et al. (2002) investigated the influence of seasonal and environmental factors on odour emissions from a livestock lagoon using statistical methods. The factors being analyzed included effluent temperature, air temperature, relative humidity, wind speed, and effluent concentration of hydrogen sulfide and ammonia. The results showed that these factors are highly related to gaseous odour emissions from livestock farms. Powers and Bastyr (2004) conducted research on climatic effects to the perception of odour downwind from poultry and livestock facilities. It was found out that wind speed would affect concentrations of phenol, decane, and undecane; and solar cover would influence odour 15 in the way that odour dilution threshold are greater on sunny days while concentrations of hydrogen sulfide and acetic acid are lower. Janes et al. (2005) proposed a multi-component, multi-factor model for the analysis of livestock farm odours using neural-networks. The location of measurement (livestock barn source or livestock manure storage source) is considered as a contributing factor to downwind odour concentrations. The results of the study showed improved accuracy on prediction of odour concentrations. These multi-component, multi-factor studies demonstrate that the accuracy and precision of odour strength prediction can be improved by taking into consideration contributing factors such as environmental conditions, type of facilities, and odour prevention operations. A possible multi-component, multi-factor neural network model for livestock farm odour is shown in Figure 1.1. 2.3 Odour Calibration Algorithms The chemical components and contributing factors need to be analyzed to determine their underlying relationships with odour strength. The analysis methods used can be conveniently subdivided into two basic types: statistical approaches which seek to probabilistically formulate the underlying relationships of the input and the output data, and non-statistical approaches which try to simulate to the human cognitive process to solve the problems (Gardner and Bartlett, 1999). Researchers have obtained considerable experience in the use of various statistical and non-statistical pattern recognition technologies. These include the use of conventional statistical techniques, artificial neural networks, fuzzy logics, and genetic algorithms. 2.3.1 Statistical Methods Statistical methods, such as linear or nonlinear multiple regression models, have been the primary tools used to analyze and calibrate the relationship between chemical components, contributing factors and odour strengths (Janes et al., 2004; Bereznicki et al., 2010). They are relatively easy to use and fast in computing. Principal components analysis (PCA) (Hanumantharaya et al., 1999; Brambilla and Navarotto, 2010), 16 Odor components Amonia Hydrogen Sulfide Acetic Acid Indole Environmental Conditions Temperature Wind speed Odour Concentration Humidity Location Type of Facility Farrowing Gestation Nursery Finishing Figure 2.1: A multi-component multi-factor model for livestock farm odour. 17 discriminant function analysis (DFA) (Chung et al., 2003; Brambilla and Navarotto, 2010) and cluster analysis (CA) (Pearce et al., 2003) are commonly used statistical methods for characterizing and identifying chemical compounds in livestock odours. Also, when large amounts of data are available, PCA and DFA are often used to pre-process data and identify useful information. However, statistical methods are sensitive to noises in the data. In addition, they are not good at incorporating nonnumerical or subjective human expert knowledge, such as influence of environmental factors and type of facilities, to help calibrate the perception of odour. Thus they are not well suited for dealing with vague and imprecise information available in odour measurement. 2.3.2 Neural Network Based Methods Artificial neural networks (ANNs) have been used to calibrate odour compounds and strength levels. The most popular type of ANN employed is the feed forward, multi-layer perceptron (MLP) training using the error-correction, back-propagation algorithm (BP) (Sohn et al., 2003; Janes et al., 2004). Hobbs et al. (2001) used a radial basis function (RBF) neural network approach to develop a relationship between odour concentration and major odorants from livestock wastes. This method could achieve good prediction result, and showed higher accuracy when compared with prediction results from a linear regression model. Carmel et al. (2003) employed a Hopfield neural network to identify chemicals and determine their concentration for an electronic nose. The algorithm was tested against real field data, and showed high quality performance, and could identify chemicals which were not previously presented to the enose system. Janes et al. (2004) used a fast back-propagation (fast BP) method to train a MLP neural network. The inputs of the neural network are concentrations of hydrogen-sulfide and ammonia, and measurement locations. The calibration outcomes were compared with those from a statistical model, the result showed that the ANN approach significantly improved odour prediction accuracy. Pan et al. (2006) developed a multi-component, multi-factor neural network model for pig farm odours, and used a structural learning with forgetting (SLF) approach to ana18 lyze potential contributing components and factors of pig farm odour. By using this method, during the training, unnecessary neural connections would fade away, and skeletal neural network would emerge, revealing relative contributions of each input (components and factors) to the outputs (odour strength). This approach would allow identification of significant chemical components and major contributing factors, and potentially help improve efficiency of odour control methods. Sohn et al. (2009) evaluated the capability of a gas sensor array system to monitor the odour abatement performance of a biofiltration system for piggery buildings. Three calibration algorithms: PCA regression, ANN and partial least squares (PLS) were employed in quantification of the sensor array responses. It showed that ANN and PLS yields good quantification accuracy while PCA regression approach was least accurate. Sun et al. (2008) applied two ANN techniques: BP neural networks and generalized regression neural network (GRNN) to quantify the diurnal and seasonal gas concentrations and emission rates from livestock facilities. Both approached demonstrated high accuracy and good systemic performance. GRNN showed fast training speed and relatively easy network parameter settings in comparison to the BP neural network method, thus was more preferred in modelling livestock farm odour. Men et al. (2011) presented a optimized competitive neural networks (CNNs) to classify odour information gathered electronic noses. The proposed CNN could automatically adjust (increase, divide, or delete) the number of neurons involved the learning, and the learning rate changes according to the variety of training times of each sample. In comparison to the traditional CNN, the new method demonstrated its ability to optimize network structures and achieve good classification results. It is widely accepted that there are many advantages in using ANNs algorithms over statistical techniques. These advantages include ANNs’ generalization abilities, their ability to model highly non-linear and complex systems, and their ability to automatically learn the relationship that exits between the inputs and outputs (Haykin, 1999). However, neural networks have some disadvantages as well. For example, they can not explicitly model human expert knowledge and non-numeric data which arise from several different sources such as environmental conditions, manure 19 systems, and type of livestock facilities. As a result, ANN algorithms sometimes fail to produce a good prediction. 2.3.3 Fuzzy Logic Based Methods Fuzzy logic utilizes fuzzy sets and fuzzy reasoning to mimic human reasoning in the computer programming. Fuzzy sets provide a way to represent fuzzy knowledge, which allow intermediate values to be defined between conventional values such as true/false and high/low. allows intermediate values to be defined between conventional evaluations such as true/false and high/low (Zadeh, 1965; Zadeh and Bellman, 1977). Fuzzy logic based methods can incorporate human experience and expert knowledge, and handle ambiguous and uncertain data. Furthermore, they are capable of dealing with linguistic information. Limited research has been reported on applying the fuzzy logic based approach to odour evaluations (Francesco et al., 2001; Huang and Miller, 2003; Jatmiko et al., 2009). Francesco et al. (2001) developed a fuzzy logicbased pattern recognition system for an conducting polymer sensors based electronic nose. In this method, the shapes of sensor responses to odorants were transformed into fuzzy linguistic expressions. Time space and signal space are fuzzified, e.g. the time interval during which odour samples were taken and the amplitude values of the signal were represented by triangular fuzzy sets. The output of the system was classified odorant. Experimental results showed that it could achieve 87% recognition rate on unknown odorants. Huang and Miller (2003) applied a multiple criteria decision making approach based on fuzzy scales to evaluate swine odour management strategies. The strategies been evaluated included manual oil sprinkling, auto oil sprinkling, web scrubber, draining shallow pit weekly, and so on. The objectives of the odour management practice are to maximize reduction in odour emissions, and to be constrained with limited financial resources. The experimental results showed that as the fuzziness scale of each strategy’s membership function varies, the preference of each strategy to the objectives of odour management practice is changed. Havlerson et al. (2007) proposed a method to assess odour annoyance using fuzzy set theory to handle ambiguity in the data. Personal reaction and past experience are 20 considered as inputs, and are fuzzified by various membership functions. The output is odour annoyance level. The predicted annoyance from this method achieved good agreement with outcomes of human expert assessments. Despite their abilities to handle vagueness, uncertainty, and linguistic information, fuzzy logics alone do not have learning ability, thus it is difficult to analyze complex systems without prior knowledge on the system being analyzed. 2.3.4 Other Methods Other methods have also been employed in calibrating odour strengths. These methods include fuzzy clustering neural network (FCNN) (Karlik and Yksek, 2007) and genetic algorithm optimized fuzzy neural (GA-FNN) system (Kusumoputro and Arsyad, 2005). Karlik and Yksek (2007) developed a novel fuzzy clustering neural network (FCNN) algorithm for recognizing odours in real-time. In this method, an unsupervised classifier called fuzzy c-means clustering is used on top of a multi-layer perceptron (MLP) network to derive the activations of input neurons. The number of input data points is reduced by fuzzy c-means clustering before they are presented to MLP, therefore the training time of the MLP is reduced. In addition, the data points are fuzzified by the fuzzy clustering, divided into fuzzy partitions, and are optimized by fuzzy c-means algorithm. Experimental results showed that FCNN can provide high recognition probability in determining odour categories, and can recognize more odours than a typical MLP network. Kusumoputro and Arsyad (2005) used a genetic algorithm (GA) to optimize the network size of a fuzzy neural system. The system was applied to classify mixture of odours. The objective of GA is to minimize activated neuron connections, error rate, and training epochs. The performance of the optimized system was compared with that of a conventional MLP neural system trained with back-propagation (BP) algorithm and a un-optimized fuzzy neural system. It demonstrated the GA-optimized fuzzy neural system has better performance in recognition capability compared to the other two systems. 21 2.4 Air Dispersion Modelling Detecting and accurately locating odour around livestock farms has been an elusive goal of researchers and environmental agencies. Air dispersion models are mathematical tools to estimate the impact of downwind odour concentrations on the basis of source factors such as odour emission rates, topography and meteorological data. Various types of dispersion models have been developed to simulate how chemical compounds dispersed in ambient air. Air dispersion models usually can be categorized into two types: steady state models such as Gaussian models, and non steady state models such as Fluid dynamics (FD) models and Lagrangian particle trajectory theory. 2.4.1 Fluid Dynamics Models Fluid dynamics models apply fluid mechanics to solve and analyze problems that involves fluid flows. The basis of fluid dynamics models are advection-diffusion equations that describes the temporal-spatial dynamics of a conservative or nonconservative pollutant (Lin et al., 2009; Hong et al., 2011). They are based on mass conservation equation, and the theory that chemical contaminants are transported in air via two processes: advection by the air flow, and turbulent diffusion. A general basic advection-diffusion equation for modelling air dispersion is given as (Brown, 1990; Zannetti, 1990) ∂C ∂t =− ∂(Vx C) ∂x + ∂ ∂x + ∂(Vy C) ∂y Kx ∂C ∂x + + ∂ ∂y ∂(Vz C) ∂z Ky ∂C ∂y + ∂ ∂z Kz ∂C ∂z + MSC − MDP , (2.1) where C is the concentration of the pollutan; Vx , Vy and Vz are the velocity components; Kx , Ky and Kz are the diffusion coefficients; MSC is the source term that represents the mass flow-in; and MDP is mass consumption term that represents the deposition and biochemical interaction effects. The first term on the right side of Eq.(2.1) is the advection contribution and the second term represents the diffusion 22 effect. This basic advection-diffusion equation provides a generally valid description of air flow. Many air dispersion models which describe the actual distribution of pollutants in the wind field are derived from this basic advection-diffusion equation. These advection-diffusion models can be solved either by analytical methods if some further assumptions are made to simplify the models and parameters. Gaussian plume model is an example of this approach (which is discussed in next section); or by using numerical methods (Zannetti, 1990; Holmes and Morawska, 2006). Various numerical approaches have been employed to solve the advection-diffusion equations. These numerical methods include finite volume (FV) methods (Gao and Niu, 2007; Tsai and Chen, 2004), finite difference (FD) methods (Meerschaert et al., 2006; Fatehifar et al., 2008), and finite element (FE) methods (Ei-Awad, 2002; Kamiski et al., 2007). Fatehifar et al. (2008) developed a finite difference approach to model the dispersion of pollutants from a network of industrial stacks. A three dimensional advection-diffusion equation was developed to describe the activity of pollutants. Assumptions made in this approach include: diffusion in wind direction is negligible in comparison to advection in that direction, and there is no wind velocity at vertical and crosswind direction. Diffusion coefficients were selected based on Pasquill’s stability class (Pasquill, 1974). Environmental parameters needed for deciding diffusion coefficients include: ambient temperature, air pressure, air viscosity, air density, wind velocity, day-time solar radiation, cloud cover, earth surface type, boundary layer height, etc. Computer simulation results showed that dispersion of pollutants is proportional to air temperature and inversely proportional to wind velocity. Tsai and Chen (2004) used a finite volume approach to solve a three-dimensional dispersion model for simulating the dispersion of air pollutant in an urban street canyon. Parameters needed were turbulent kinetic energy, turbulent energy dissipation rate, and Stefan-Boltzmann constant. Values of dispersion parameters were obtained according to (Launder and Spalding, 1974). Simulation results were compared with measurement results, and predicted pollutant concentration were of high accuracy. Kamiski et al. (2007) used a finite element method to model the threedimensional dispersion of carbon monoxide along roads. Environment parameters 23 considered were wind speed and direction, temperature, pressure, air humidity, cloud cover, and traffic velocity. A software was developed based on this method, and field data was collected for validating the model. Calculated results based on the approach agreed well with measured levels of carbon monoxide. However, numerical approaches are complicated, a number of initial conditions and boundary conditions which are difficult to obtain in the field are in need to solve advection-diffusion equations. And usually many assumptions are made to simplify the solution. Numerical approaches require high computational and memory capacity. Even with simplified models and high-speed computers, only approximate solutions can be achieved in many cases. 2.4.2 Gaussian Dispersion Models Gaussian dispersion models are used for air dispersion in a steady-state environment. They are a closed solution of the advection-diffusion equation. They are relatively easy to use, and require small number of inputs. The basic Gaussian plume model is the corner stone in most dispersion models. It is based on following assumptions (Beychok, 2005; Schnelle and Dey, 1999): 1. Vertical and crosswind diffusion follow Gaussian distributions; 2. The source emissions are continuous and the mass-flow rate (i.e. g/s)is constant; 3. The horizontal wind velocity (i.e. m/s) is constant and the mean wind direction is constant; 4. Diffusion of emissions in the downwind direction is negligible compared to their transport by the wind; and 5. There is no deposition or washout of emissions, no absorption of emissions by the ground or other physical bodies, and no chemical conversion of the emission. 24 The basic Gaussian plume model was initially developed for a continuous point source in a uniform flow with homogeneous turbulence, which is expressed as y2 Q exp − 2 c̄ (x, y, z, H) = 2πūσy σz 2σy (z − H)2 (z + H)2 exp − + exp − 2σz2 2σz2 , (2.2) where c̄ (x, y, z) is the steady-state concentration of pollutant at a specific point (g/m3 ); Q is the source’s strength or emission rate of the pollutant (g/s); H is the effective source height; ū is the mean transport velocity across (m/s); x is the downwind distance from the source to the receptor (m); y is crosswind distance from the receptor to the plume centreline (m); z is vertical distance from the receptor to the centreline (m); σy is the horizontal dispersion coefficient; and σz is the vertical dispersion coefficient. For the centreline concentration of ground-level odour source, the value of z = H = y = 0, therefore the model becomes c̄ (x, 0, 0) = Q . 2πūσy σz (2.3) The dispersion coefficients σy and σz are empirical parameters depending on air stability classes, and commonly they are functions of the downwind distance from the source to the receptor. The air stability classes defines the atmospheric turbulence that occurs in the ambient air (Barratt, 2001). The most commonly used air stability classes categories are the Pasquill stability classes, which are defined by wind speed, daytime incoming solar radiation, and night-time cloud cover. The Pasquill stability classes are presented in Table 1.1 (Pasquill, 1961). Air stability is considered to have a major effect on the dispersion of air pollution plumes by many researchers, because it enhances the plume dispersion (Barratt, 2001; Beychok, 2005; Hoff and Bundy, 2003). By using Pasquill air stability classes, verticalσz and lateral σy dispersion coefficients are expressed as functions of distance from the source and Pasquill-Gifford curves, and can be computed as follows σy = 465.11628(x) tan(θ), (2.4) σz = axb . (2.5) 25 Table 2.1: Pasquill-Gifford air stability classes (from Pasquill, 1961) Surface Daytime Night-time wind speed Incoming solar radiation Cloud cover (m/s) strong moderate slight > 50% < 50% <2 A A-B B E F 2-3 A-B B C E F 3-5 B B-C C D E 5-6 C C-D D D D >6 C D D D D Pasqui-Gifford stability class Definition A very unstable B unstable C slightly unstable D neural E slightly stable F stable 26 However, Pasquill dispersion coefficients are based on field experiments and are only appropriate when used for modelling air dispersion in open, rural areas (Pasquill, 1976). Gaussian dispersion models have been widely used in evaluation odour impacts around livestock facilities and in determining set-back distance between livestock farms and local residents. Chastain and Wolak (2000) adopted a Gaussian plume model to predict odour concentration downwind from the odour source, and used it to assist selection of sites of livestock facilities. The dispersion coefficients were selected based on air stability classes defined by (Turner, 1994). Surface roughness was considered. Urban empirical dispersion parameters were used for forested areas, and open terrain dispersion coefficients were used for cut grass or crop area. Sensitivity analysis was performed on primary input variables to provide objective basis for selecting livestock facilities locations. Analysis results also showed that forest barriers and odour source control can help reduce odour dispersion. Hayes et al. (2006) used a commercial Gaussian atmospheric dispersion model to evaluate to evaluate odour impact of existing intensive poultry farms. The model was also used to determine setback distance of new poultry production units. Pasquill stability classes were used. It was assumed that the surrounding terrain was uniformly flat, therefore, topological data was not used in this study. A new odour annoyance criterion was developed in the study, and it suggested the new odour annoyance criterion could largely reduce the maximum setback distance of new poultry facilities. Latos et al. (2010) developed a Gaussian dispersion model for odorous chemical compounds hydrogen sulfide and ammonia emitted from pig farms. In this study, in order to predict the maximum concentration with a single breath’s time-scale (few seconds) instead of a long-term average concentration, dispersion coefficients were modified based the “peak-to-mean”. Complex terrain around the farm and the height of the emission stack were considered. The analysis results indicated that a standard Gaussian dispersion model that predicts long-term average concentration underestimates the impact of odorous pollutant, and short-term “peak-to-mean” modification should be adopted in odour dispersion model. Yu (2010) developed a livestock farm odour dispersion 27 model based on Gaussian plume theory. Pasquill-Gifford dispersion coefficients were used for long-time odour emission, and Hogstrom dispersion coefficients were applied for modelling short-time odour fluctuation. Atmospheric parameters such as friction velocity, sensible heat flux, and mixing height were considered. Also, predicted odour concentration was modified by “peak-to-mean” ratio in order to reflect short-term peak odour concentration during a single breath’s time. The model showed its capability to predict mean odour concentration, instantaneous odour concentration, peak odour concentration and frequency of odour concentration. The model was tested against field odour plume measurement data, and achieved good odour concentration and odour frequency predictions. Li (2009) evaluated impact of odorous emissions from livestock farms using two Gaussian model based commercial air dispersion models: AERMOD and CALPUFF. Sensitivity analysis of the two models showed that out of five major climatic parameters, under steady-state condition, air stability class and wind speed had greatest impact on odour dispersion, while ambient temperature and wind direction had little impact, and mixing height had no impact on odour dispersion. The two models were validated using field odour data, and it was found that both models had poor performance in modelling dispersion of low strength livestock farm odours. Since Gaussian models are build upon assumptions that the dispersion field is in steady-state conditions, the accuracy of Gaussian models depends upon the validity of the those assumptions against the environment being analyzed. Therefore, their applications to real world problems are greatly limited. For instance, by assuming the dispersion filed is non-variable, Gaussian models exclude the effect of pollutant emissions and environment factors from previous hours. They are more suitable for modelling air dispersion in relatively simple meteorological and topographical conditions, such as flat field without complex ground surface conditions. However, in rural areas where most livestock facilities are locate, topographical conditions are usually complicated with farm facilities, hills, rivers, forests, and various vegetation heights. Gaussian dispersion models are not suitable for modelling the dispersion of odour emissions around livestock farms. 28 When applying existing air dispersion models to model livestock odour dispersion, the livestock odour is treated as a chemical pollutant which follows mass conservation law. However, downwind odour strength is not only resulted from physical dilution process of chemical compounds, but is also a result of biological olfactory sensing process. Livestock farm odour consists of over 100 odorous compounds. Each compounds has different odour perception threshold. During dispersion, the concentrations of some compounds fall below their perception thresholds, e.g. the quantity of odour is not conservable even if there is no chemical reaction among the chemical compounds. Therefore, the odour perceived by human panel at a downwind location is different than the odour at source given the distance to the source is far enough, because there are less perceivable odorous compounds in the downwind odour. This would influence odour dispersion modelling at longer distance range where many compounds of livestock farm odour are at their perception thresholds. Therefore, most existing air dispersion model which are based on mass conservation law are not suitable for modelling livestock odour dispersion. 2.4.3 Lagrangian Particle Models Lagrangian particle models have been applied to simulate livestock farm odour dispersion (Duyzer et al., 2004). In a Lagrangian model, the odorous emissions released from a source are assumed to be a cloud of particles, where a particle represents a pollutant mixture. Lagrangian models compute the random spatial trajectories of the particles following a statistical approach. Then the odour concentration is calculated as the total number of particles falling in a unit volume (Lin et al., 2011). However, Lagrangian particle models require extensive computing time and memory resource. To adequately modelling odour concentration around a livestock farm, the trajectories of several tens of thousands of particles have to be simulated simultaneously in small consecutive time steps. Therefore, it leads to very time-consuming computing, which are not practical for real-time odour monitoring application. 29 Chapter 3 A Wireless Electronic Nose Network for Monitoring Livestock Farm Odour Odour control technologies are greatly impeded by the lack of science-based approaches to assess the effects of odour. With the current state of technology, the most commonly used methods to measure odours from livestock facilities is human sniffing methods, but they are very expensive and difficult to overcome the analyst’s personal bias. Therefore, unbiased automatic odour evaluating technologies are desirable. 3.1 Introduction An electronic nose is an alternative tool to human nose for objectively measuring livestock farm odour. Electronic noses have been developed for monitoring agricultural environment (Osarenren, 2002; Qu et al., 2001; Hudon et al., 2000; Pan and Yang, 2007). In our previous study, an electronic nose consisting of a sensor array and an analysis system was developed, and proved its capability of producing a qualitative output, lower operation cost, and greater consistency in odour measurement (Pan and Yang, 2007). The impact area of odorous gas emissions is difficult to de30 termine because it continuously changes with wind speed and direction. In addition, a modern livestock facility is usually not a point odour source but rather an array of points (ventilation hose), lines (side curtain opening) and areas (manure pits). However, an electronic nose can only evaluate odour events at one location; it cannot provide a comprehensive odour impact evaluation according to farmstead factors such as building layout schemes, landscape and topography around the farm, and meteorological factors, which has led to an unfocused and inefficient research approach on reducing livestock farm odour. Therefore, an odour measurement technology that can provide an overall odour profile around livestock facilities is desirable for developing an overall odour management strategy. it cannot provide an comprehensive odour map around livestock facilities which is necessary for an effective odour management strategy. Wireless communication technology has been widely applied in environmental observations (Wang et al., 2006; Werner-Allen et al., 2006), medical diagnostic (Koizumi et al., 2008; Greer et al., 2008), habitat monitoring (Mainwaring et al., 2004), military applications (Zhao et al., 2006; Liang, 2006; Song and Hatzinakos, 2007) and various industries (Basset et al., 2007; Yi, 2008). Disseminating data via wireless network enables long-term, remote data collection, and can provide localized measurements and detailed information that is hard to obtain through traditional instrumentation (Tubaishat and Madria, 2003; Wang et al., 2006). In this research, a novel wireless electronic nose network system is proposed for monitoring livestock farm odours. The system utilizes an electronic nose network built from compact electronic noses which can detect odour compounds and environment conditions such as temperature and humidity. The electronic noses are placed at various applicable locations on the farm, and odour data collected by them are delivered via a wireless network to the analysis station, typically a laptop computer, where several data analysis algorithms process and analyze the data, display sensor signals in real-time, and model the odour dispersion. A software package is also developed for performing all the data analysis, and for providing a platform for collaborations among the users. 31 The development of the proposed wireless electronic nose network system includes following parts: design of several simplified electronic noses for detecting odour components and environmental conditions, network architecture design, design of a software package to process and analyze odour data, development of data processing and analysis algorithms, and integration of the whole network. In this chapter, the design of the entire electronic nose network is presented. Section 3.2 firstly introduces the architecture of the proposed wireless electronic nose network system; the development of each single electronic nose is given in details in Subsection 3.2.1; Subsection 3.2.2 presents the set-up of the wireless network; and the software developed for the electronic nose network are covered in Subsection 3.2.3. At the end, several advantages of the proposed electronic nose network are addressed in Subsection 3.3.3. 3.2 The Developed Wireless Electronic Nose Network System In this study, an electronic nose network-based system is developed for monitoring livestock farm odour remotely. The system utilizes an electronic nose network built from compact electronic noses which can detect odour compounds and environment conditions such as temperature and humidity. The architecture of the proposed wireless electronic nose network is illustrated in Fig. 3.1. Essentially, the wireless electronic nose network is a simple star network, with all the sensor nodes communicating with a central control station. It consists of 3 electronic noses at this time, and can be extended to accommodate more electronic noses when necessary. The electronic noses are placed at various location of interest on the farm, and odour data collected by them are delivered via wireless link to the analysis station, where the sensor fusion algorithms process and analyze the data. Usually if an electronic nose fails, other electronic noses cover that area can also provide the missing information. Therefore, the more electronic noses in a certain area, the more robust the wireless network system is to the sensor failure. However, a larger density of electronic noses 32 User Interface Tire Sensor Control Gateway Data Analysis Data Display Data Manage User Database Tier Data Acquisition Wireless Connection Electronic nose Electronic nose Electronic nose Electronic nose Figure 3.1: The architecture of the proposed wireless electronic nose network. costs more, and large redundant information makes wireless transmission slow. The proposed wireless electronic nose network consists of various hardware components. In this section, the architecture of the wireless electronic nose network and the development of essential hardware components are presented. 3.2.1 Development of the Electronic Noses To be utilized in the wireless network, the electronic noses should be compact in size, energy efficient, and of low cost. The design toward the final electronic nose of the wireless sensor network comprised several steps such as the selection of sensors to detect compounds most prevalent in livestock farm odour, the incorporation of these sensors into a sensor array, the design of a chamber to regulate gas toward the sensors and the acquisition of the sensor data for wireless transfer. 33 Sensor Selection and Sensor Array Each electronic nose in the network has a sensor array with 4 metal-oxide semiconductor (MOS) gas sensors to detect odorous components in ambient air, and 2 sensors to detect environmental conditions, i.e., a humidity sensor and a temperature sensor. The gas sensors are selected based on previous extensive investigation and experiments, as well as various sensors available for odour compounds measurement. These 4 sensors can measure most of the major gas compounds in livestock farm odours, with high sensitivity to target gases, quick response to low concentrations of gases, high stability and reliability over a long period, relatively low cost and long life in comparison to other sensing technologies. A section of this sensor list is shown below in Table 3.1. The selected gas sensors are purchased from Figaro Engineering Inc. The gas sensors are fixed in an array. A developed sensor array is shown below in Fig. 3.2. Table 3.1: Sensors used in the development of the electronic nose Sensors Compounds Material TGS826 ammonia, amine compounds, toluene tungsten dioxide TGS825 hydrogen sulfide tin dioxide TGS822 broad spectrum including skatole, indole, methane, tin oxide benzene, ethanol, acetone, butane, propane, alcohol, etc. .. . .. . .. . 34 Figure 3.2: The developed sensor array. Design of the Chamber A chamber is designed to accommodate the sensor array and to regulate the flow of odorous air. The sensor array is placed within this chamber, and the odorous air is directed toward the array. Since the sensors have specific requirements on the flow rate for proper operation, a pump is used to control the flow speed. The sensor array faces directly into the coming air so that the surface area of the array which is exposed to odorous air is maximized. This ensures best detecting results and minimizes the possibility of attaining improper readings. The chamber also has an input valve which is controlled by a solenoid. The valve is switched between odorous and carbon filtered air ports through the application a 12 V signal to the valve, so that the filtered odorous air can refresh the chamber and sensor when necessary. The pump used is a standard high-volume, low-pressure pump which ideally suited our requirement for low-pressure, continuous sampling, and allows the air to become steady in the chamber. The chamber also contains a simple diffuser to focus the incoming odorous and filtered air onto the sensor array. A developed chamber is shown in Fig. 3.3. 35 Figure 3.3: The developed chamber. Integration of the Electronic Nose The analog sensor signals collected by the electronic nose are converted to 12 bit digital signals by a data acquisition board (MDA300) right at the electronic nose side. The multi-function data acquisition board MDA300 can convert up to 11 channels analog input. The digital signals are delivered by a 2.4 GHz mote processor (MPR2400) to a gateway (the sink). Both the data acquisition board MDA300 and the mote MPR2400 are powered by two AA batteries. In addition, a 12 V DC power supply is used to power the gas sensors and the air pump. A developed electronic nose is shown in Fig. 3.4. These electronic noses are placed in areas of interest around livestock facilities to collect odour data. 3.2.2 The Wireless Electronic Nose Network The odour data collected by the electronic noses located around the farm is delivered by 2.4 GHz wireless link to a gateway. The gateway will gather the sensor signals and input them into the control centre via Ethernet. The block diagram of the wireless network is shown in Fig. 3.5. For the purposes of wireless transmission of sensor signals, in this work, the MicaZ wireless network platform from Crossbow (Crossbow Technology Inc., USA) is 36 Figure 3.4: An electronic nose consists of the sensor array inside a chamber, a data acquisition board, a mote processor, and a power supply. Mote MPR 2400 Mote MPR 2400 Wireless Communication DAQ MDA 300 Gateway MIB 600 Ethernet Sensor Node Internet Global Connection Odorous Air Figure 3.5: The block diagram of the wireless network. 37 adopted. This wireless network platform has advantages of the small physical size, low cost and low power consumption, making it ideal for this odour monitoring application. The mote processor (MPR2400), and the gateway (MIB600) are utilized as shown in Fig. 3.6. Figure 3.6: The mote processor MPR2400 and the gateway MIB600. The mote processor MPR2400 includes an ATmega128L low-power micro-controller, a high data rate radio, and batteries. The micro-controller runs network communication applications from its internal flash memory. A single MPR2400 processor can support sensor control, data processing and radio communication applications simultaneously. Power is provided by any 3 V power source, typically two AA batteries. The radio on the MPR2400 module consists of an antenna and a single-chip 2.4 GHz radio frequency transceiver which is compliant to IEEE 802.15.4/ZigBee protocol. The transceiver providing a spreading gain of 9 dB and an effective data rate of 250 kbps. The MIB600 is an Ethernet interface board. It provides Ethernet connectivity for data communication ability via TCP/IP. A MIB600 board attached with a MPR2400 38 is defined as a getaway (sink). It allows the aggregation of in-coming network data into a computer. The gateway with connection to a laptop computer is shown in Fig. 3.7. The wireless link provides communication ability to transfer sensor data and status information across the network, it also support remote control of network nodes. Figure 3.7: The gateway with connection to a laptop computer. 3.2.3 Software Integration In order to utilize the previously mentioned hardware, certain software packages are needed. An application provided by Crossbow called MoteWorks is used to set up a wireless network for data transfer. Then MoteWorks integrates and synchronizes the wireless network, configures and acquires the IP address of the network, and pro39 grams the mote to interface with the electronic noses. An application software called MoteView from Crossbow is used to establish the network and wireless communication, monitor the network status, and manage the network topology. A screen of the wireless network topology interface is shown in Fig. 3.8. The odour data collected by the electronic nose network need to be processed and analyzed for the purpose of supporting odour control decisions. Hence, a software suite is designed for data acquisition, management, display and analysis. The software is running on a central analysis station, and it includes four main components: (1) a data acquisition system to acquire the sensor signals that are transferred to the computer via the wireless network; (2) a database which stores the odour data for future applications; (3) an odour analysis system, which identifies risk factors of odour, calculates odour strength and odour dispersion plume; and (4) a user interface tier interacts with users and displays odour data and reports analysis results. Data Acquisition System After analog sensor signals are converted into digital signals, the digital signals are sent through wireless link to the gateway, which receives and collect the digital signals. Then the data acquisition system in the computer interfaces with the getaway and captures the digital signals into the database. The data acquisition interface is shown in Fig. 3.9. Communication to the gateway can be established by specify the IP address of the gateway. The status of the connection is shown on the interface. In this data acquisition system, currently there are 9 channels, a channel shows electronic nose node ID, a temperature sensor channel, a humidity sensor channel, four channels adc0 to adc3 are used for the 4 gas sensors in each electronic nose, and channels adc5 and adc6 are reserved for future development. Sensor signals generated by the electronic nose usually are contaminated by white noise. An exponentially weighted moving average filter is used to minimize the noise (Smith, 1997). The filter is given as x̄k = n n x̄k−1 + (1 − )xk , n+1 n+1 40 (3.1) where n is number of data points in the filter; k is the k-th data point; and x̄k is a simple moving average filter given by x̄k = k 1 xi . n i=k−n+1 (3.2) Database and Data Management System All collected data are organized and stored in a central database. Database plays an important role as a data storage, query, and exchange centre for various applications. In this system data structures (records and files) are optimized to deal with large amounts of data. A data storage system based on Microsoft SQL Server 2005 database server is developed for storing sensor signals and odour information. The database is organized and managed by a database management system. The stored odour data in the database can be retrieved, managed, and processed through user interface. The interface of the database management system is shown in Fig. 3.10. The data management system has link to the data acquisition system. Therefore, the data acquisition system can be conveniently launched from the data management system to capture odour data into database. Collected data records are then structured, categorized, and tagged in the database for future use. Because data records are well-organized, historical data can easily be queried and retrieved by name, date and time of collection, description, and characters. A picture of data records management console is shown in Fig. 3.11 User Interface Tier Users interact with the control station directly through a series of user interfaces. These interfaces are within the user interface tier. The goals of user interfaces are to provide a user-friendly environment, and to adequately support the operation undertaking. User interfaces provide graphical front-end pages to the data base records, analysis result reports, task management, and the other components. Microsoft C# environment is used for user interface development. The main interface is the first displayed page when users start the application. Users log onto the system from this 41 Figure 3.8: Topology of the wireless network. Figure 3.9: The interface of data acquisition system. 42 Figure 3.10: The interface of database. 43 Figure 3.11: The interface of data records management console. page to get the access to other functional pages such as data display interface and analysis system interface. Figure 3.12 shows the main interface of the application. To satisfy various requirements, the user interface tire can provide a widely used standard for analyzing record data and also accommodates applications developed in other programming language environment. The interface of data display system is shown in Fig. 3.13. To provide better odour strength observation, a data dynamic display system is developed. This system provides basic sensor signal on-line and off-line display. Several display attributes can be selected by users, such as different update interval, number of visible points and data range, persistent display mode enable/disable, average number display, etc. This would be very helpful for routine operation and various studies. Odour Analysis System Odour analysis system is the key component of the software. The system utilizes the data analysis applications to identify rules and patterns in the data, to tell the 44 Figure 3.12: The main interface of the software. 45 Figure 3.13: The dynamic display console. users where is the odour, how strong the odour is, to give suggestion on odour control practice, and to predict the resulted odour strength if any odour control means are applied. The data analysis system has many sub-systems: odour strength analysis system calculates odour concentration/intensity using artificial intelligent techniques; odour dispersion analysis system calculates odour dispersion plume using odour dispersion models based on odour emission rates, topological and meteorological information; risk factor analysis module identifies significant risk factors which cause odour problems; and decision making module assists users to make odour control decisions based on analysis results and human expert knowledge. The interface of odour analysis system is shown in Fig. 3.14 This analysis system also provides decision support for farmer’s odour control practice. In the analysis system, the sensor fusion algorithm calculates the odour strength based on collected sensor signals, odour dispersion algorithm calculates and draws the odour dispersion. The odour dispersion analysis system provides an overall odour mapping around live facilities which is necessary for an overall odour manage- 46 Figure 3.14: The odour analysis system. ment strategy, and it also gives suggestions on possible odour control practice based on farmstead, topography and meteorological data, such as when to spread manure, change vent direction, and where to plant trees to block odour. This will help the livestock producers to make strategic funding decisions by investing only in odour reduction research and implementation that deals with relevant factors of the odour problem. 3.3 Discussion The proposed electronic nose-based wireless network approach to automatically monitoring of livestock farm odours differs from any existing electronic noses in the following ways 1. The developed wireless electronic nose network system can provide multi-location odour monitoring while existing electronic noses can only provide odour monitoring at a single location. Detecting and accurately locating odour around livestock farms has been an elusive goal of researchers and livestock producers for a long time. Odour concentration changes with meteorology, distance to the source, emission height above ground, and source configuration (exhaust pipe diameter, building geometry etc). The proposed wireless electronic nose 47 network can provide a more comprehensive odour plume profile than a single electronic nose. 2. The developed electronic nose network can automatically monitor livestock farm odours, and have many potential industry applications. It integrates sensor system, data acquisition system, data analysis system, data transmission systems, and user interface system. The developed wireless network can perform remote multi-point odour monitoring, and the integrated software package provides data analysis capabilities which would assist users’ decision making on odour control practice. Based on meteorological information provided by weather forecast, the software can estimate future impacts of odours around the farm, and help farmers plan odour control strategies. In addition, if the odour strength reaches a pre-set threshold, the network system can alerts farmers, and provide suggestions on reducing odour levels. The electronic network system can provide farmers and researchers with more efficient odour control capabilities by providing automated, robust, and comprehensive data about the environment and odour profile around livestock farms. 3. By using this wireless electronic nose network system, users can monitor the odour from a remote location such as inside a house or office, so that they do not have to stay in field, which makes odour measurement easier in unfavourable weather. 4. The developed electronic nose network system with multiple electronic noses is more robust to sensor failure and noise. One or more failed or incorrect sensor reading could be corrected through the redundant information from the other electronic noses. The developed wireless electronic nose network system is a useful tool in remote monitoring livestock farm odour, which can greatly improve the efficiency of livestock farm odour reduction and thus benefit livestock industry. 48 3.4 Summary The design of an electronic nose network-based livestock farm odour monitoring system is presented. The system is composed of electronic noses that can detect odour compounds and environment factors. The electronic noses are deployed around the farm and odour data are delivered via a wireless network to the central analysis station. The sensor fusion algorithm and odour dispersion algorithm in the analysis system calculate the odour dispersion, display the odour plume dispersion dynamically, and predict odour plume. By using the proposed wireless electronic nose network based remote odour monitoring system, real-time downwind odour measurement becomes possible. The developed real-time odour monitoring system can enable the accurate detection and location of a livestock farm odour plume. It should be effective in quantifying the instantaneous and long term odour strength. The odour monitoring and analysis system can provide farmers and researchers with more accurate odour management capabilities for more efficient operation of odour control systems such as ventilation fans, and can help the development of an overall odour management strategy by providing dynamic, comprehensive information about the livestock farm environment and odour dispersion. 49 Chapter 4 A Neuron-fuzzy Model for Odour Calibration Calibration of odour concentration against human panelists’ perception is difficult due to the complexity of the livestock farm odour system and limited knowledge of the odour itself (Zhang et al., 2002c). Many factors influence odour strength. Research has indicated that environmental conditions such as temperature and humidity (Powers and Bastyr, 2004), type of facilities (Janes et al., 2004), and farm management practises affect odour generation (Zhang et al., 2002). However, how and by how much a factor would influence livestock farm odour still remains unclear (Schiffman et al., 2001a; Powers and Bastyr, 2004; Pan et al., 2006). Resulting from a poor understanding of the odour problem, existing odour calibration technologies are not efficient. 4.1 Introduction Most previous studies on modelling livestock farm odour have concentrated on the modelling of the odour components and have not considered other potential contributing factors, such as outdoor temperature, air flow speed, or type of facilities, due to the limitation of statistical modelling techniques. In statistical methods, odour system models being analyzed are not capable of incorporating non-numeric or subjective 50 human expert knowledge, such as environmental conditions, type of livestock facilities. Thus they are not well suited for dealing with vague and imprecise information available in odour measurement and control. The rapid development of soft computing techniques, such as neural networks and fuzzy logic technologies, allows the incorporation of those non-numeric contributing factors and expert knowledge into livestock farm odour models. Artificial neural networks (ANNs) have been used to calculate odour strength (Pan et al., 2006; Kodogiannis et al., 2008; Al-Bastaki, 2009). It is widely accepted that there are several advantages in using ANNs algorithms over the conventional techniques, such as its generalization ability to automatically learn the relationship that exits between the inputs and outputs. However, ANNs are not capable of properly incorporating non-numeric or subjective human expert knowledge (Haykin, 1999). Fuzzy logic utilizes fuzzy sets and fuzzy reasoning to provide a more human-like inference process. It can handle imprecise, ambiguous, and vague information, and can incorporate human experience and expert knowledge (Zadeh and Bellman, 1977). Fuzzy logic has been used in a wide range of applications such as control systems, project management, non-linear system models, expert systems, forecasting, system identification and analysis (Zimmermann, 2001). However, fuzzy logic does not have learning ability, thus it is difficult to analyze complex systems without prior and accurate knowledge on the system being analyzed. To overcome the limitations of neural networks and fuzzy systems, an adaptive network-based fuzzy inference system (ANFIS) approach is proposed to predict livestock farm odour concentration. Two ANFIS livestock farm odour models are developed, both numeric data and non-numeric data are incorporated in the odour models. Various comparative studies are also presented to demonstrate the effectiveness of the proposed method. 51 4.2 The Proposed Neuro-fuzzy Approach In common fuzzy logic systems, membership functions are chosen by trial and error and are fixed once selected. Rule structure of a fuzzy logic system is also predetermined based on user’s interpretation of the variables in the model. However, in practise, due to limited knowledge about the system being analyzed, the selection of membership functions and rule structure may not be appropriate, thus results in poor performance of the model (Jang et al., 1997). An adaptive neuro-fuzzy inference system (ANFIS) approach is proposed for analyzing livestock farm odour. The proposed ANFIS approach incorporates an adaptive neural learning technique into the fuzzy logic model. The parameters are tuned automatically during the learning so the membership functions can well represent the nonlinear system being studied with an optimal performance. The proposed ANFIS uses a Sugeno-type fuzzy relation (Jang et al., 1997; Karray and De Silva, 2005), where the fuzzy rules take the following form: Rule 1: IF x1 is A11 and x2 is A21 . . . and xn is An1 , THEN f1 = a10 +a11 x1 +...+a1n xn , Rule 2: IF x1 is A12 and x2 is A22 . . . and xn is An2 , THEN f2 = a20 +a21 x1 +...+a2n xn , .. . Rule p: IF x1 is A1m and x2 is A2m . . . and xn is Anm , THEN fp = ap0 + ap1 x1 + ... + apn xn , where xi , i = 1, ..., n is i-th input linguistic variable in the antecedent part of the rule; variable Aij is the j-th linguistic label (for instance, large, small, etc.) associated with xi . Function fp is the consequent output of the rule and api is the Sugeno parameter. An general ANFIS model is shown in Fig. 4.1, which has n inputs and each input variable has m membership functions, resulting in a total of mn rules. There are 5 layers in the general ANFIS model shown in Fig. 4.1. The first layer is a fuzzification layer, in which the crisp inputs of the network are fuzzified through mapping into corresponding membership functions. Layer 2 is a rule operation layer. In this layer, the incoming signals of each rule node are multiplied and the product is sent out. The output of each node (e.g. the product of the incoming signals of the 52 Layer 1 μA (x 1 ) 11 x 1 μA (x 1 ) 1m μA (x 2 ) 21 x A111 A Layer 2 A122 A Π A A1m2 Π B211 A w1 Layer 3 N N Π N Layer 4 w1 Σ w2 Σ Layer 5 w1 f 1 Odour Concentration w3 Σ A222 B 2 μA (x 2 ) 2m μA n1(x n ) x n μAnm(x n ) AB2m Bn11 A An22 B Π N Π N wm n wmn-1 wmn Σ wmn f mn ABnm2 Figure 4.1: The structure of an ANFIS consists of a five-layer feedforward network. 53 node) in layer 2 is referred as the firing strength of a rule. Layer 3 is a normalization layer, the firing strengths of the fuzzy rules are normalized in the way that, the output of each node in this layer is the ratio of the i-th rule’s firing strength to the sum of all rules’ firing strength. Layer 4 is a consequent layer, in which the values of the consequent of the rules are multiplied by normalized firing strengths. The last layer is aggregation layer. The overall output of this layer is computed as a summation of all incoming signals. During the training, the membership parameters can be tuned automatically in a systematic manner so as to adjust the membership functions of the input/output variables for optimal system performance. Layer 1: Fuzzification Layer In this layer, the crisp inputs xi , i = 1,...,n, is fuzzified through mapping into membership function μAij of the linguistic labels Aij , i = 1,...,n, j = 1, ..., m. The membership functions can be any appropriate parameterized functions. In this study, all the membership functions are bell-shaped functions as μAij (xi ) = 1+ 1 xi −cij aij 2bij , i = 1, 2, ..., n; j = 1, 2, ..., m, (4.1) where aij , bij and cij are the parameters; n is the number of inputs; and m is the number of membership functions of each input. The bell-shaped functions vary accordingly when these parameters are changed, and thus exhibit various forms of membership functions on Aij . The output Op1 of each node i in layer 1 is the membership functions of Aij . Op1 = μAij (xi ), i = 1, 2, ..., n; j = 1, 2, ..., m; p = 1, 2, ..., m × n, (4.2) where variable Op1 represents the degree to which the given xi associates with Aij . Layer 2: Rule Operation Layer In this layer, each rule node multiplies the incoming signals and sends the product out. The output of each node in layer 2 represents the firing strength of a rule, which 54 is given as Op2 = wp = n i=1 ji = 1, 2, ..., m; p = 1, 2, ..., mn , μAiji (xi ), (4.3) where j1 , j2 ,..., jn is a combination of m membership functions for the n input variables, thus there are a total of mn combinations, resulting in mn nodes in this layer. Each node in fact performs and AND operation within the rule antecedent. Other t-norm operators can also be used as the node function in this layer. Layer 3: Normalization Layer In this layer, the firing strengths of the fuzzy rules are normalized. The output of each node in this layer is the ratio of the i-th rule’s firing strength to the sum of all rules’ firing strength, and is calculated based on wp Op3 = wp = mn p=1 wp , p = 1, 2, ..., mn . (4.4) Layer 4: Consequent Layer In this layer, the values of the consequent are multiplied by normalized firing strengths according to Op4 = wp fp , p = 1, 2, ..., mn . (4.5) Layer 5: Aggregation Layer The overall output of this layer is computed as a summation of all incoming signals: n 5 O = m p=1 4.3 wp fp = p wp fp . p wp (4.6) Experimental Results The proposed ANFIS approach is tested by a livestock database collected in the fields downwind from poultry, dairy and swine facilities. This section provides detailed information about the data samples. Three odour calibration models are developed for comparative studies. The structure of each model is also presented in this section. 55 4.3.1 Data Sets Outdoor temperature, wind speed, distances to the odour source, and odour dilutionto-threshold (D/T) concentration levels perceived by human assessors were recorded. At the same time, an electronic nose took measurement at each site. The sensor signals from the electronic nose represent the concentrations of gas compounds in the air. The detailed measuring procedure is described in the paper by (Pan et al., 2006). The data of temperature, type of facility and concentration of hydrogen sulfide are used in odour generation modelling in this study. Factors such like wind speed and distance to odour source are not considered as they mainly influence downwind transportation of odour emissions. By using statistical multi-variant linear regression method, temperature and concentration of hydrogen sulfide being independent variables and odour concentration being dependent variable, a low accuracy (r = 0.40, p < 0.01) is obtained on prediction of odour concentration. It is not surprising that low accuracy is achieved using multi-variant linear regression method, as livestock farm odour systems yield highly non-linear character. There are a total of 64 samples in the data available for the development and testing of models. Approximately 80% of the data points are randomly selected training the neural network, and the remaining 20% are used for testing. 4.3.2 Livestock Odour Calibration Models Three models are developed based on the database mentioned above: an ANFIS model with only 2 numerical inputs, a second ANFIS model with a linguistic input and 2 numerical inputs, and a neural network model with 2 numerical inputs. Model 1: an ANFIS Model with Two Inputs To make comparative study, an ANFIS model (Model 1) which contains only numeric inputs is developed. These inputs include concentration of chemical components that is represented by the output signals of sensors and environmental conditions such as temperature. Researchers have indicated that the concentration of hydrogen sulfide 56 can act as an indicator for livestock farm odour strength (Lunn and Van De Vyver, 1977; Williams, 1984). The concentration of hydrogen sulfide is represented by the magnitude of the output signal of a sensor that is selected for detecting hydrogen sulfide. All the inputs are numeric data that have to be fuzzified in the fuzzification layer. The output of Model 1 is the odour concentration. Three membership functions are associated with each input, thus resulting in 9 IF-THEN rules in the model. Model 1 is trained by a back-propagation (BP) algorithm (Haykin, 1999). Model 2: an ANFIS Model with Three Inputs In order to test the ability of the proposed approach of incorporating linguistic information, another ANFIS model (Model 2) is developed. The Model 2 has identical inputs as those of model 1, but with 1 more non-numeric input: type of facilities. The type of facilities can generally represent such measures as type of animals, farm activity, odour emission rates, and other physical measures that may influence the dour concentration. The output is the odour concentration. Model 2 also has 3 membership functions for each input, resulting in 27 IF-THEN rules in total. Model 2 is illustrated in Fig. 4.2. The model is trained by BP algorithm. Model 3: a Typical Neural Network Model In order to analyze the effectiveness of the proposed approach, a feedforward neural network (NN) model (Model 3) is designed with an input layer, one hidden layer, and an output layer. A three-layer neural network is theoretically able to learn any nonlinear relationship at a desired level of precision with sufficient number of hidden neurons in the network architecture and through sufficient learning. Figure 4.3 illustrates the proposed network structure for livestock farm odour. Model 3 has identical inputs as those of Model 1. The output is the odour concentration. The neural network is trained by a typical BP algorithm, and the results are compared with the results from Model 1 and Model 2. 57 Layer 1 Layer 2 Layer 3 Layer 4 Π N Σ Π N Σ Π N Π N Π N Layer 5 Dairy Type of facility A Swine 2 Poultry 2 Cold1 Temperature Odour Concentration Σ B Medium 2 Hot B Low1 B H2S 2 Medium High2 B Σ Figure 4.2: An ANFIS model with three inputs and 27 IF-THEN rules for calibrating the livestock farm odour. Input layer Hidden layer Output layer Temperature Odour Concentration H2S Figure 4.3: Structure of NN model for calibrating the livestock farm odour. 58 4.3.3 Results In this section, the results of proposed ANFIS approach are analyzed. In order to demonstrate the effectiveness of the proposed approach, the odour concentration prediction results from both ANFIS models are compared with that from NN model. In neuro-fuzzy technology, parameters such as learning rate and training epochs, and the number of membership functions for each input are usually determined by trial and error. In this study, the sensitivity of these parameters are investigated and showed following results. (1) With each increment of the training epoch, the accuracy of odour concentration prediction improves. After 100 epochs, the curve of RMSE tends to stabilize. (2) The number of membership functions for each input reflects the complexity of ANFIS for tuning parameters. If the number is too small, it is not enough to approximate the complex relationship of data; if the number is too large, it is produces the redundancy for the nonlinear relationship of data. Three membership functions for each input variable results in highest accuracy of odour concentration prediction in this study. (3) The higher the learning rate, the faster the network converges. However, if the learning rate is too large, the network oscillates. In this study, a learning rate of 0.1 is selected by trial. Performance of Model 1 The performance of models are evaluated based on the accuracy of odour concentration prediction and root mean squared error (RMSE) of the predicted odour intensities. By using the proposed ANFIS approach, the odour concentration prediction accuracy is significantly improved (correlation coefficient r = 0.946; RMSE = 1.11) compared to that from Model 3. The result demonstrated that the proposed ANFIS model can incorporate human experience and knowledge, which allows prior knowledge to be embedded in the model, and this improve the results of learning. The comparison of initial membership functions before the learning with final membership functions after learning using Model 1 is shown in Fig. 4.4. The membership functions are all changed during the learning. The result showed that the proposed 59 ANFIS approach is capable of tuning the membership functions to improve the performance index. Performance of Model 2 By incorporating type of facility, the prediction accuracy is improved (r = 0.976; RMSE = 1.03) compared to those of Model 1 and Model 3. The comparison between the initial membership functions before the learning and the final membership functions after learning using Model 2 is shown in Fig. 4.5. The parameters of type of facilities are changed during the learning. This result shows that type of facility does affect odour concentration. The proposed ANFIS approach is able to interpret linguistic variables such as type of facility and cloud cover conditions, and therefore increase the accuracy of odour concentration prediction. Factors such as temperature and type of facility are actually measured or known exactly before the analysis. Their data are not fuzzy. However, by fuzzifying these data and applying ANFIS, the predicted odour concentrations are of higher accuracy than those by using classical or crisp methods (multi-variant linear regression or neural network). By using fuzzy sets and fuzzy reasoning, the proposed method is able to break stiffness and inflexibility that lie in accurate and precise classical or crisp logic, and make fuzzy logic more “soft” and dexterous. Performance of Model 3 The correlation coefficient of the predicted odour concentration and perceived odour concentration is 0.522 by using neural network technique alone. The RMSE is 7.18. The predicted odour intensities are not as accurate as those of Model 1 and Model 2. 4.4 Summary The calibration of odour strength around livestock facilities has been investigated by many researchers over past decades. Calibration of the odour concentration is difficult due to limited knowledge on livestock farm odour itself. It is known that 60 Membership 1 0.8 0.6 0.4 0.2 0 0 Cold 1 0.8 0.6 0.4 0.2 0 0 Low Hot Medium 5 10 15 20 25 Temperature, C 30 35 High Medium 25 50 40 75 100 125 150 175 200 225 250 Concentration of H2S, ppm Membership (A) 1 0.8 0.6 0.4 0.2 0 0 Cold 1 0.8 0.6 0.4 0.2 0 0 Low Medium 5 25 10 15 20 25 30 Temperature, C Medium 50 Hot 35 40 High 75 100 125 150 175 200 225 250 Concentration of H2S, ppm (B) Figure 4.4: Comparison of the initial membership functions before the learning (A) with the final membership functions after learning (B) using Model 1. 61 Dairy 1 0.5 0 Swine Membership Dairy 1 0.5 0 0 Cold 1 0.5 0 0 Low Swine Facility Type Poultry Poultry Medium 5 10 15 20 25 30 Temperature, C Medium 25 50 Hot 35 40 High 75 100 125 150 175 200 225 250 Concentration of H 2S, ppm Membership (A) 1 0.5 0 Dairy 1 0.5 0 0 Cold 1 0.5 0 Low Swine Dairy 0 Swine Facility Type Poultry Poultry Hot Medium 5 25 10 15 20 25 30 Temperature, C Medium 50 75 35 40 High 100 125 150 175 200 225 250 Concentration of H 2S, ppm (B) Figure 4.5: Comparison of the initial membership functions before the learning (A) with the final membership functions after learning (B) using Model 2. 62 a livestock farm odour system is a highly non-linear system that is influenced by many odorous components and various factors such as environmental conditions and type of facilities. The rapid development of soft computing techniques provides more opportunities for improving accuracy of livestock farm odour modelling. An adaptive neuro-fuzzy based approach which incorporates neuro-adaptive learning techniques into fuzzy logic method is developed to analyze livestock farm odour. This approach can incorporate non-numeric data and subjective human expert knowledge, which allows prior knowledge to be included in the model. In addition, the membership functions are tuned during the learning according to input/output data, therefore to optimize the performance. This feature is important as it can avoid inappropriate predetermination of membership functions, thus minimize errors resulted from the limited knowledge about the livestock farm odour system. A livestock farm odour database is used in this study. Several livestock farm odour models are developed, both numeric data and non-numeric data are incorporated in the odour models. Various comparative studies are presented to demonstrate the effectiveness of the proposed method. The results show that the proposed approach is effective and provides a much more accurate odour prediction in comparison with a typical multi-layer feedforward neural network. 63 Chapter 5 A Neuron-dynamics Model for Odour Dispersion The concentration of livestock farm odours in the field is influenced by meteorology conditions, distance to the source, and farmstead layout (e.g., source configuration). As livestock farms have special odour emissions, farmsteads, and odour management systems, an ad-hoc dispersion model for livestock farm odours can greatly improve the efficiency and accuracy of odour dispersion modelling. 5.1 Introduction No air dispersion models have been developed specially for modelling livestock farm odour. Most used air dispersion models like US EPA regulatory models ISCST3 and Australian model AUSPLUME are initially designed to predict the concentration of pollutants from industrial sources. The empirical dispersion parameters they adopt are developed for modelling dilution process of industrial pollutants in ambient air, not for odour emissions. However, downwind odour strength is not only resulted from physical dilution process of chemical compounds, but is also a result of biological olfactory sensing process. These dispersion models may represent the dispersion of gas compounds, but are not very suitable to accurately model perceived odour concentration as a whole. 64 In this study, a novel neural network model is proposed for modelling the dynamic odour dispersion around livestock farms. The proposed neural network model for livestock farm odour dispersion is motivated by Hodgkin and Huxley (1952) biological membrane model and the later developed Grossberg’s shunting model (Grossberg, 1973; Grossberg, 1982; Grossberg, 1983; Grossberg, 1988). Hodgkin and Huxley (1952) initially proposed a shunting model for a patch of membrane in a biological neural system using electrical circuit elements (Hodgkin and Huxley, 1952). The dynamics of voltage across the membrane Vm can be described using state equation technique as Cm dVm = −(Ep + Vm )gp + (EN a − Vm )gN a − (EK + Vm )gK , dt (5.1) where Cm is the membrane capacitance; EK , EN a and Ep are the Nernst potentials for potassium ions, sodium ions and the passive leak current in the membrane, respectively; and gK , gN a and gp represent the conductances of potassium, sodium and passive channels, respectively. This model together with Hodgkin and Huxley’s other experiments led them to a Nobel prize in 1963. Based on Hodgkin and Huxley (1952)’s biological membrane model (Eq. (5.1)), Grossberg proposed a shunting model to describe the stable adaptive behaviour in real time to complex and varying environmental events (Grossberg, 1973; Grossberg, 1988). It has a lot of applications in biological and machine vision, sensory motor control and some other area (Li and Ogmen, 1994; Ogmen and Gagne, 1990a; Ogmen and Gagne, 1990b). Substituting Cm = 1, ξi = Ep + Vm , A = gp , B = EN a + Ep , D = Ek − Ep , Si+ = gN a and Si− = gK in Eq. (5.1), Grossberg obtained a typical shunting model characterized as dξi = −Aξi + (B − ξi )Ii+ (t) − (D + ξi )Ii− (t), dt (5.2) where ξi is the neural activity (membrane potential) of the i-th neuron; A is a positive constants representing the passive decay rate; B is the upper bound of the neural activity; and D is the lower bound of the neural activity. Variables Ii+ and Ii− are the excitatory and inhibitory inputs to the neuron, respectively. 65 The shunting model in (5.2) shows that the excitatory input Ii+ and inhibition input Ii− are multiplied by two auto gain control terms, (B − xi ) and (D + xi ), respectively, which depend on the activity ξi (t). The interaction of excitatory and inhibitory inputs are nonlinear via multiplication. In addition, (5.2) shows that the activity of the cell, ξi , increases at a rate of (Bi − ξi )Ii+ . Hence, the cell activity ξi is forced to stay below the upper bound Bi . Similar to the inhibitory term, it forces the cell activity stay above its lower bound −Di . Therefore, once the activity goes into the [−Di , Bi ] region, it is guaranteed that the cell activity stays in this region for any excitatory and inhibitory inputs (Li and Ogmen, 1994). 5.2 The Proposed Neuro-dynamics Approach Based Grossberg’s shunting model in Eq. (5.2), a dynamic odour dispersion model in two dimensions is proposed as ∂C(Xi ,t) ∂t = −AC(Xi , t) + (B − C(Xi , t))[I(Xi , t)]+ + N j=1 wij [C(Xj , t)]+ (5.3) − − (D + C(Xi , t))[I(Xi , t)] , where Xi = (x, y) is the spatial location of the i-th neuron; x is the downwind distance of the i-th neuron to the odour source, while y is the crosswind distance of the i-th neuron to the odour source; C(Xi , t) is the odour concentration at location Xi and time t; [I(Xi , t)]+ and [I(Xi , t)]− are excitatory and inhibitory inputs, respectively, where [e]+ and [e]− are linear-above threshold functions defined as ⎧ ⎪ ⎨ [e]+ = max {e, 0} ⎪ ⎩ [e]− = max {−e, 0} . (5.4) Parameters Arepresent the passive decay rate; B is the upper bound of the neural activity; and D is the lower bound of the neural activity. Parameters B and D are constants, and A can be a function of wind speed, i.e. A = qVW (t), 66 (5.5) where q is a positive constants; and VW (t) represents the wind speed at time t. Therefore, parameter A represents the transportation effect of air flow, the higher the air flow speed, the faster the odorous compounds being dispersed. The variable I(Xi , t) is external input to the i-th neuron defined as ⎧ ⎪ ⎪ ⎪ S(t), ⎪ ⎪ ⎨ I(Xi , t) = if there is a odour source at positionXi −R(t), ⎪ ⎪ ⎪ ⎪ ⎪ ⎩ 0, if there is odour reduction means at position Xi , (5.6) otherwise where S(t) and R(t) are positive functions of time. The odour reduction means includes odour control practise operated by livestock producers, dry deposition to the earth’s surface, and wet deposition by precipitation (rain, fog, snow, etc.). In Eq. (5.15), item N j=1 wij [C(Xj , t)]+ represents the interaction among the neigh- bouring neurons; N is the total number of neighbouring neurons of the i-th neuron; wij is the connection weight from j-th neuron to the i-th neuron, which is a function of distance defined as ⎧ ⎪ ⎨ 0, wij = ⎪ ⎩ if dij > r0 u/dij , if dij ≤ r0 , (5.7) where r0 is a positive constant; and dij = |Xi − Xj | represents the Euclidean distance between positions Xi and Xj in the state space. Therefore, the neuron has only local lateral connections in a small region (0, r0 ). Function u(θ) is a function of wind direction (θ), which is defined as θ u(θ) = −E cos( ), 2 (5.8) where θ is the angle between the wind direction and the position of the neighbour neuron. When 0 ≤ θ ≤ 90, the neighbour position Xj is downwind of Xi , the position being inspected, therefore, odour emissions are transported from position Xi to Xj , which causes a depletion of odour concentration/intensity at position Xi ; when 90 < θ ≤ 180, the neighbour position Xj is upwind of Xi , therefore, odour emissions are transported from position Xj to Xi , which results in a replenishment of odour concentration/intensity at position Xj ; When θ = 180 or θ = 0, the position Xj is right 67 on the centreline of the wind direction, therefore, the odour concentration/intensity at Xi yields a maximum replenishment/depletion effect. The emission rate from the odour source, the environment (wind direction and speed, etc.), and farming activities (spreading manure, applying odour reduction means, etc.) may vary with time. The activity landscape of the neural network dynamically changes due to the varying external inputs and the internal neural connections, and therefore dynamically representing the changes of odour dispersion. 5.3 Stability Analysis of the Proposed Dispersion Model The proposed neural network is a stable system, where the neural activity is bounded in a finite interval [-D, B] (Grossberg, 1982; Grossberg, 1983; Grossberg, 1988). In addition, the stability and convergence of the proposed neural network system can be rigorously proved using a Lyapunov stability theory. From the definition of Ii− , Ii+ , and wij , Eq. (5.15) can be rewritten into Grossberg’s general form (Grossberg, 1988), ⎛ ⎞ n dxi = ai (xi ) ⎝bi (xi ) − cij dj (xj )⎠ , dt j=1 (5.9) by the following substitutions xi = Ci (Xi , t), ai (xi ) = B − xi , 1 bi (xi ) = − AB + (B − xi )(A + [Ii ]+ ) + (D + xi )[Ii ]− , B − xi cij = −wij , dj (xj ) = [xi ]+ . Since B and D are positive constants, and xi = Ci (Xi , t) ∈ [−D, B], where D = 0 by definition, then ai (xi ) ≥ 0 (positivity); since wij = wji , then cij = cji (symmetric); from the definition of function [e]+ , the function dj (xj ) = [xj ]+ has a nonnegative derivation, i.e. dj (xj ) ≥ 0 (monotonicity). Therefore, the proposed neural network in 68 Eq. (5.15) satisfies all the three stability conditions required by Grossberg’s general form (Grossberg, 1988). The rigorous proof of the stability and convergence of the general form in Eq. (5.9) can be found in Grossberg (1983). Therefore, the proposed odour dispersion system is stable. The dynamics of the network is guaranteed to converge to an equilibrium state of the system. 5.4 Simulation Results The proposed neural network model is capable of dynamically modelling odour dispersion in an arbitrary environment. In the simulation study, the proposed model is applied to odour dispersion of a livestock farm. Several cases are studied: odour dispersion in a static environment with various kinds of odour sources (point, line, area, and irregular area sources), odour dispersion in a dynamic environment, and odour dispersion in sudden environmental changes. In the following, these simulations results are outlined. The simulation results show that the proposed model is capable of describing odour dispersion in various environments. 5.4.1 Odour Dispersion in Static Environments In this section, the proposed model is applied to solve the odour dispersion problem in static environment in various cases. In all of the cases studied here, the wind speed is assumed to be 2.5 m/s, odour source height and receptor height are assumed to be the same, the ground roughness is assumed to be 0.1 m(typically low grass on the ground), and there is no rain drop. Odour Dispersion from a Point Source The proposed model is applied to simulate the odour dispersion from a point source, typically a ventilation hose. The simulated odour dispersion is shown in Fig. 5.1A. The odour strength along the centreline of the odour plume simulated by using the proposed model is shown in Fig. 5.1B, and the odour dispersion along crosswind direction is shown in Fig. 5.1C. 69 Odour concentration (DT) 60 48 36 24 12 20 ind sw os Cr 0 0 ) (m 40 200 150 100 50 0 Downwind distance (m) (A) 3.6 Odour concentration (DT) 60 3.0 48 2.4 36 1.8 24 1.2 12 0 0.6 0 50 100 150 200 0 0 5 10 15 20 25 30 35 Downwind distance (m) Crosswind distance (m) (B) (C) 40 45 Figure 5.1: Odour dispersion from a point source simulated by the proposed model. (A) Odour dispersion from a point source; (B) Odour dispersion along the centreline of the odour plume; (C) Odour dispersion along crosswind direction. 70 It is believed by many researchers that without obstacles crosswind diffusion should occur according to Gaussian distributions. Many atmospheric dispersions models such as Gaussian air dispersion models are developed under this assumption (Beychok, 2005; Schnelle and Dey, 1999). In this study, the Shapiro-Wilk test is applied to test whether the crosswind dispersion generated by the proposed shunting model is in accordance with Gaussian distribution. The test results are shown in Fig. 5.2. It is clear that the crosswind distributions simulated by the proposed model follow Gaussian distribution. Odour Dispersion from a Line Source The proposed model is applied to simulate the odour dispersion from a line source, typically side curtain opening of a barn. The simulated odour dispersion is shown in Fig. 5.3. Odour dispersion from a Regular Area Source The proposed model is applied to simulate the odour dispersion from an area source, typically a manure pit. The simulated odour dispersion is shown in Fig. 5.4. Odour Dispersion from an Irregular Area Source The proposed model is applied to simulate the odour dispersion from an irregular area source, typically manure spread. The simulated odour dispersion is shown in Fig. 5.5. 5.4.2 Odour Dispersion in Non-static Environments In this section, the proposed model is applied to solve the odour dispersion problem in non-statistic environment in various cases. 71 2.0 Expected Normal Value Expected Normal Value 2.5 1.5 1.0 0.5 0.0 2.5 2.0 1.5 1.0 0.5 0.0 -0.5 -1.0 -1.5 -1.5 -2.0 -2.0 -2.5 -2.5 -0.0050.000 0.005 0.010 0.015 0.020 0.025 0.030 0.0350.040 -0.002 0.002 0.006 0.010 0.014 0.018 0.022 0.026 Observed Value Observed Value -0.5 -1.0 (B) 2.5 2.0 1.5 1.0 0.5 0.0 -0.5 -1.0 -1.5 -2.0 -2.5 0 1E-8 1.2E-8 1.4E-8 Expected Normal Value Expected Normal Value (A) 2.5 2.0 1.5 1.0 0.5 0.0 -0.5 -1.0 -1.5 -2.0 -2.5 0 2E-9 4E-9 6E-9 8E-9 Observed Value 2E-28 6E-28 1E-27 1.4E-27 Observed Value 1.8E-27 (D) (C) Figure 5.2: Gaussian distribution test on the crosswind distributions generated by the proposed shunting model. (A) 50 m away from source (SW-W = 0.819, p ¡ 0.001); (B) 100 m away from source (SW-W = 0.897, p = 0.0014); (C) 150 m away from source (SW-W = 0.920, p = 0.0067); (D) 200 m away from source (SW-W = 0.909, p = 0.0031). 72 Odour concentration (DT) 60 48 36 24 12 0 0 50 30 100 150 Downwind distance (m) 200 0 40 20 10 Crosswind distance (m) Figure 5.3: Odour dispersion from a line source simulated by the proposed model. Odour concentration (DT) 60 48 36 24 12 0 0 50 30 100 150 Downwind distance (m) 200 0 40 20 10 Crosswind distance (m) Figure 5.4: Odour dispersion from a regular area source simulated by the proposed model. 73 Odour concentration (DT) 60 48 36 24 12 0 0 50 30 100 150 Downwind distance (m) 200 0 40 20 10 Crosswind distance (m) Figure 5.5: Odour dispersion from an irregular area source simulated by the proposed model. Odour Dispersion from an Instantaneous Source So far the odour sources studied above are continuous sources that are emitting continuously or for time periods equal to or greater than the travel times from the source to the point of interest. Cases of instantaneous release, as from short-term releases on the order of seconds, are also of practical concern. The proposed model is applied to simulate the odour dispersion from an instantaneous emission. The simulated odour dispersion is shown in Fig. 5.6. Odour Dispersion in Changing Wind Speed The proposed model is applied to simulate the odour dispersion in changing wind. The wind speed is increasing linearly as a function of time. The comparison of odour plume centrelines when wind speed are at 1.74 m/s, 1,77 m/s and 1.8 m/s is shown in Fig. 5.7. From the simulation result, it is obvious that the odour level is lower at higher wind speed, while at low air flow rate, the odorous 74 Odour concentration (DT) 24 18 12 6 0 0 50 Downwind distance (m) 100 0 10 20 30 40 Crosswind distance (m) Figure 5.6: Odour dispersion from an instantaneous emission simulated by the proposed model. particles are dissipated slower and not as far. This result is agree with that observed in downwind odour measurement on livestock farms (Pan et al., 2007). Wind helps transportation of odours; odours are dissipated faster as wind speed increases. 5.5 Experimental Results The proposed odour dispersion model is tested using a livestock database. Odour data samples were collected in the fields downwind from poultry, dairy and swine facilities in 2004 by Ontario Ministry of Agriculture and Food. At each farm, human assessors walked around the farm, identifying wind direction and odour plume. Measurements were taken at various locations within the odour plume. Outdoor temperature, wind speed, distances to the odour source, and odour dilution-to-threshold (DT) concentration perceived by human assessors were recorded. Types of odour sources include ventilation fans, vents, manure pits, and combinations of above sources. Maximum odour concentration is 60 DT, and minimum concentration is 0 DT. Totally 120 data 75 Odour concentration (DT) 60 Wind speed at 1.74m/sec Wind speed at 1.77m/sec Wind speed at 1.8m/sec 50 40 30 20 10 0 0 20 40 60 80 100 Downwind distance (m) Figure 5.7: Odour dispersion in changing wind speed simulated by the proposed model. sets were collected, where 43 were from dairy farms and 77 were from swine farms. In the proposed approach, parameters B and D are the upper and lower bounds of the neural activity, respectively. In this study, the upper limit of odour concentration is 60 DT and the lower limit of odour concentration is 0 DT (e.g., no odour is detected by human panel). In computer simulation, odour values are normalized between 0 and 1, and B=1 and D=0 are chosen for all cases. The value of other parameters such as a, u, and d are adjusted by trial and error to minimize the differences of odour concentrations calculated by the neural network model and observed by human panel. As the result, a is selected between 0.8 and 3, the value of u is between -0.5 and 2 √ and the value of d is between 0 and 2. The performances of models are evaluated based on the accuracy and root mean squared error (RMSE) of the calculated odour concentration in comparison to the experimental odour concentration perceived by human panels. The neuron grid is scaled to the real odour field, and the calculated values from the neuron network are also scaled up to 0 DT and 60 DT accordingly using linear regression. By using the proposed odour dispersion model, the accuracy 76 of the calculated odour concentration can reach correlation coefficient r = 0.606 and RMSE = 12.04 DT in comparison to human perceived odour levels. The calculated odour concentrations using the proposed approach are in close accord with the experimental data. The results show that the proposed approach is effective in calculating odour dispersion around livestock farms. 5.6 Discussion Above simulation results demonstrated that the proposed odour dispersion model has several advantages over commonly used air dispersion models, such as computational fluid dynamics models, Gaussian models and Lagrangian models. 5.6.1 Comparison to Gaussian Models Gaussian dispersion models have been proved to be useful tools in predicting odour concentration downwind of an odour source in a stationary environment (Beychok, 2005; Schnelle and Dey, 1999). The basic Gaussian plume model is initially developed for modelling emissions from point sources. The formula of the basic Gaussian plume model is given below: Q y2 C (x, y, z, H) = exp − 2 2πūσy σz 2σy (z − H)2 (z + H)2 exp − + exp − 2σz2 2σz2 , (5.10) where C is the odour concentration; Q is the emission rate at the point source; ū is the mean transport velocity across the plume; σy and σz are the Gaussian plume dispersion parameters; H is the height of the odour source; x is the downwind distance to the odour source; y is the crosswind distance; and z is the vertical distance. Performance in Static Environments To make comparative studies, the proposed odour dispersion model and a typical Gaussian dispersion model is applied to simulate odour dispersion from a point odour source in static environment. The odour dispersion alone the centreline of the odour 77 Odour concentration (DT) 60 Calculated by Shunting model Calculated by Gaussian model 50 40 30 20 10 0 0 20 40 60 80 100 120 140 160 180 200 Downwind distance (m) (A) 1.8 Calculated by Shunting model Calculated by Gaussian model Odour concentration (DT) 1.6 1.4 1.2 1.0 0.8 0.6 0.4 0.2 0.0 -20 -15 -10 -5 0 5 10 Crosswind distance (m) 15 20 (B) Figure 5.8: Comparison of odour dispersion from a point source downwind along plume centreline (A) and along crosswind direction (B), using the proposed model and a typical Gaussian dispersion model. plume simulated by both the models is shown in Fig. 5.8A, and the odour dispersion along crosswind direction is shown in Fig. 5.8B. In Fig. 5.8, the calculated odour concentrations along both downwind direction and crosswind direction by the two methods yield very close results. This demonstrates that the proposed odour dispersion model, in static regime, can achieve similar results and accuracy as traditional Gaussian dispersion models. However, difference is observed in Fig. 5.8A: the odour concentration calculated by the proposed model decreases to 0 quickly at a distance around 120 m while the odour concentration 78 predicted by the Gaussian model still decreases slowly. This is because Gaussian dispersion models are originally designed to simulate the dispersion of industry pollutants (such as ammonia and hydrogen sulfide) in air. However, those industry pollutants are different from livestock farm odours, in the way that pollutants are physical substances while an odour actually is a result from human olfactory perception. Perception of an odour is subject to a threshold which is the concentration of odorous compounds at which it is noticeable by the human nose. Therefore, once released from the source, a pollutant always exists in the air no matter how low the concentration is, but an odour may not be perceived when the concentration of odorous compounds are below the threshold. Thus, the behaviour of the proposed odour dispersion model is more consistent with our knowledge and expectations of odour diffusion processed in the environment. Capability of Modelling Odour Dispersion from Complex Odour Sources The basic Gaussian plume model is initially developed for modelling emissions from point sources (Beychok, 2005; Schnelle and Dey, 1999). When modelling emissions from line sources and area source, integration and double integration of the Gaussian plume kernel shown in Eq. (5.10) is performed respectively. To model a finite line source, the odour concentration is calculated as C (x, y, z, H) = L y2 Ql exp − 2 2πūσy σz 2σy (z − H)2 (z + H)2 exp − + exp − 2σz2 2σz2 dl, (5.11) where Ql is the line source emission rate; L is the length of line source; and dl is incremental length. To model an area source, the odour concentration is calculated as C (x, y, z, H) = S Qs y2 exp − 2 2πūσy σz 2σy (z − H)2 (z + H)2 exp − + exp − 2σz2 2σz2 ds, (5.12) where Qs is the area source emission rate; S is area of the source; and ds is incremental length. However, these integrations are complicated and require great computing effort, 79 especially when modelling an irregular odour source. Besides, when modelling odour dispersion from multiple sources, Gaussian models calculate odour dispersion from each individual source, and then the total odour dispersion is computed as the arithmetic sum of dispersions from each of the sources. This method deserves further Discussions as the odour system is a highly nonlinear system that the odour concentration at one location is not a simple sum of concentrations of individual odours at that location. The proposed odour dispersion model can easily model odour emissions from complex sources, without integrating Gaussian plume equations. The odour dispersion is represented by the landscape of the neural activity for all neurons, and the dynamics of each neuron in the topologically organized neural network is characterized by a shunting neural equation. Therefore, the proposed model is much easier and simpler than traditional Gaussian dispersion models in modelling odour dispersion from various sources. Capability of Modelling Odour Dispersion in Non-Static Environments The most significant advantage of the proposed model over traditional Gaussian dispersion models is that the proposed model can simulate odour dispersion in non-static environment. Gaussian dispersion models take assumptions that the source emissions are at a continuous and constant mass-flow rate, and the horizontal wind velocity the mean wind direction are both constant (Beychok, 2005; Schnelle and Dey, 1999). Therefore, Gaussian models are only suitable for modelling air dispersion in static environment, which greatly limits the applications of Gaussian dispersion models to solve real world air dispersion problems. Livestock farm odour emissions are mostly released by near ground sources (less than 1000 m high). Due to the surface friction near the ground, the wind speed varies with height such that Gaussian models are not appropriate (Turner, 1994). Demonstrated by the study above, the proposed model is capable of simulating the air dispersion in a dynamic environment, therefore, it is more suitable than Gaussian models to simulate livestock farm odour dispersion. 80 5.6.2 Comparison to Fluid Dynamics Models To compare the proposed method with a general 2D fluid dynamics model (FD), Eq. (2.1) can be rewritten as ∂C ∂t =− ∂(Vx C) ∂x + ∂(Vy C) ∂y + ∂ ∂x Kx ∂C ∂x + ∂ ∂y Ky ∂C ∂y + MSC − MDP , (5.13) where C is the concentration of the pollutant; Vx and Vy are the velocity components; Kx and Ky are the diffusion coefficients; MSC is the source term that represents the mass flow-in; and MDP represents mass consumption term that represents the deposition and biochemical interaction effects. The first term on the right side of Eq. (2.1) is the advection contribution and the second term represents the diffusion effect. The 2D dispersion model in Eq. (5.13) uses a fixed coordinate system that is fixed to earth, so the wind direction is variable. Therefore, Vx and Vy are both variables in this fluid dynamics model. If a variable coordinate system which is adaptive to the wind direction, is adopted by this model, such that the x-axis always points to the downwind direction and y-axis is on crosswind direction, therefore the wind direction is no longer variable, and Vy and ∂(Vy C) ∂y all become zero. In this way, Eq. (5.13) becomes ∂C ∂t = − ∂(V∂xx C) + ∂ ∂x Kx ∂C ∂x + ∂ ∂y Ky ∂C ∂y + MSC − MDP . (5.14) The above Eq. (5.14) shares some similarity with the proposed neuro-dynamics based odour dispersion model. The proposed odour dispersion model is given as ∂C(Xi ,t) ∂t = −AC(Xi , t) + (B − C(Xi , t))[I(Xi , t)]+ + N j=1 wij [C(Xj , t)]+ (5.15) − − (D + C(Xi , t))[I(Xi , t)] , where the excitatory input [I(Xi , t)]+ represents mass generation term, as the source term MSC in Eq. (5.14); the inhibitory input [I(Xi , t)]− is mass consumption term which represents the wet and dry depositions, as MDP in the FD model; the influence from neighbouring neurons N j=1 wij [Cj (Xj , t)]+ represents the diffusion effect, as the 81 second term on the right in Eq. (5.14). And the first right term −ACi (Xi , t) in Eq. (5.15) is the advection term as − ∂(V∂xx C) in Eq. (5.14). Since the proposed shunting dispersion model is based on a neuron network, it is discrete in spatial domain. If discretize the spatial domain of the FD model, the advection term − ∂(V∂xx C) in Eq. (5.14), it becomes x C+Vx C x C − V(x+1)−x = − Vx C+V = −Vx C − Vx C. 1 (5.16) Assume that the mass concentration within each discrete unit is uniform, that is C = 0 in Eq. (5.16). So the advection term in FD finally becomes −Vx C, which is very similar to the first right term AC in Eq. (5.15). As shown above, although originate from different areas, the proposed shunting approach is similar to FD models. It includes advection, diffusion, source and sink effects in the model, which describes the time and space evolution of pollutant as FD models do. However, the proposed shunting approach adopts a spatial discrete neuron network, thus by discretizing the spatial domain, it replaces first order (advection) and second order (diffusion) partial differential terms in FD models with simple multiplications. Therefore, the computing resource and time can be greatly reduced. 5.6.3 Comparison to Lagrangian Particle Models The proposed model is computationally efficient. To adequately modelling odour concentration around a livestock farm, a Lagrangian particle model would require hundreds of thousands of virtual particles be simulated simultaneously (Hurley, 1994), and the trajectories of these particles have to be calculated in small consecutive time steps. Therefore, it leads to very time-consuming simulations. Using the proposed odour dispersion model, a small neuron grid is enough to simulate odours downwind of a livestock facility. Therefore, it greatly reduced the computing efforts required in comparison with Lagrangian particle models. 82 5.7 Summary The locating and identifying odour dispersion around livestock facilities has been investigated by many researchers over past decades. Prediction of the odour dispersion is difficult due to the complex characters of odour itself, and complicated farmstead scheme, landscape and topology, and meteorology conditions around livestock facilities. In this study, an odour dispersion model is proposed to dynamically simulate odour dispersion around livestock facilities. The proposed dispersion model dynamically represents steady-state or non-steady-state environment features around livestock farms. The model can simulate odour dispersions from various types of sources such like point source, line source, and area source, and from multiple odour sources. In addition, the proposed model is relatively computationally efficient. The stability of the proposed neural network system is guaranteed by qualitative analysis and the Lyapunov stability theory. Computer simulation is performed over the proposed model. Complex and nonsteady-state environmental features around livestock farms are studied, including simulation of odour dispersions from multiple odour sources, and from various types of sources such like point sources, line sources, and area sources. Field odour data gathered from Ontario livestock farms is also used to test the proposed method. The results show that the proposed approach is effective in providing accurate odour dispersion prediction. 83 Chapter 6 Conclusions and Future Work The measurement and analysis of livestock farm odour is an important research topic in both agricultural and engineering areas, the findings from this study expand the body of knowledge in this area. This chapter gives a brief summary of the research. Future work is also discussed in this chapter. 6.1 Conclusions A wireless network based electronic system has been developed for measuring and analyzing odours around livestock facilities. The system utilizes a wireless sensor network built from electronic noses that can detect odour compounds and environment factors such as temperature, wind speed, and humidity. The electronic noses are located at various points on the farm and sensor signals are delivered via wireless links to an analyzing station, typically a laptop computer, where a sensor fusion algorithm and odour dispersion model calculate the odour strength; display the odour dispersion plume; and, if any environment factors have changed or if different control strategies have been applied, analyze the resulting odour plume. The odour monitoring and analyzing system can provide the farmer and researcher with more precise odour management capabilities for more efficient operation of odour control systems, such as ventilation fans and biofiltrations; and can help the development of an overall odour management strategy by providing dynamic, comprehensive information about 84 the livestock farm environment and odour dispersion. A multi-component, multi-factor odour calibration model is developed for adequately modelling livestock farm odour. An adaptive neuro-fuzzy based approach which incorporates neuro-adaptive learning techniques into the fuzzy logic method has been developed to analyze livestock farm odour. This approach can incorporate non-numeric data and subjective human expert knowledge, allowing prior knowledge to be included in the model. In addition, to optimize the performance, the membership functions can be tuned during the learning according to input/output data. This feature is important as it can avoid inappropriate predetermination of membership functions, thus minimizing errors resulting from limited knowledge about the livestock farm odour system. A livestock farm database collected by our research team has been used in the study. The results show that the proposed approach is effective and provides a much more accurate odour prediction than a typical multi-layer feedforward neural network. An odour dispersion model is proposed for modelling odour dispersion around livestock facilities. The proposed dispersion model dynamically represents complex or non-steady-state meteorological and topographical features in livestock farm areas. The model can also model odour dispersions in both static environments and nonstatic environments, and from various types of sources like point source, line source, and area source. This model can enable a more realistic prediction of odour dispersion, thus improving the predictions of odour impact and helping to better define the parameters for odour control options. Odour reduction research for livestock farms can then be directed toward the most significant odour impact factors, thereby increasing the efficiency of developing odour reduction technologies and methods for livestock producers, and greatly benefiting the livestock industry in Canada and elsewhere. 6.2 Future Work There remain many important and critical issues that should be explored in future research, including the following issues. 85 1. The cost efficiency of the wireless electronic nose network is an important factor. A study should be performed on reducing the price of each electronic nose as well as the whole network system. First, gas sensors and other network components that have higher accuracy, are less expensive, and have a longer life should be selected to achieve maximum measurement accuracy while keeping cost to a minimum. The number of electronic nose nodes should also be examined. If an electronic nose fails, other electronic noses usually cover that area and can provide the information lost by the nose failure. Therefore, the greater the number of electronic noses in a certain area, the more robust the wireless network system is to the sensor failure. However, a larger density of electronic noses is expensive and is more difficult to maintain, and a large redundancy in information makes wireless transmission slow. The objective of the optimization of the wireless electronic nose network is to minimize both measurement error and total cost of the network, while maximizing data transmission quality and keeping the network functional, stable and robust. 2. Continue the research on odour calibration and dispersion procedures; improve the accuracy of odour calibration and dispersion models. More field odour dispersion data need to be gathered from real livestock facilities. More farm landscape, topological and meteorological factors should be studied for validation of the dispersion model proposed in this study. Livestock farm operation methods, feeding materials and methods, and manure processing means should be considered in odour calibration models, as well as more advanced computing algorithms are for the calibration of odour levels. 86 References Al-Bastaki, Y. (2009). An artificial neural networks-based on-line monitoring odor sensing system. Journal of Computer Science 5(11), 878–882. 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Science 311(5766), 1477–1481. 100 Appendix: Selected Publications Journal Papers Published journal papers are attached after Page 102. 1. L. Pan and S.X. Yang: (2009). “An electronic nose network system for online monitoring livestock farm odors,” IEEE/ASME Transactions on Mechatronics, Vol. 14, No. 3, pp. 371-376. 2. R. Liu, L. Pan and S.X. Yang (2009). “A web-based collaborative system for remote monitoring and analysis of livestock farm odours,” Journal of Environmental Informatics, Vol. 13, No. 2, pp. 75-85. 3. L. Pan, L. Qin, S.X. Yang and J. Shuai, (2008). “A neural network based method for risk factor analysis of West Nile Virus,” Risk Analysis, Vol. 28, No. 2, pp. 487-496. 4. L. Pan and S.X. Yang, (2007). “Analyzing livestock farm odour using an adaptive neuro-fuzzy approach,” Biosystems Engineering, Vol. 97, No. 3, pp. 387393. 5. L. Pan, and S.X. Yang, (2007). “An new intelligent electronic nose for measuring and analyzing livestock and poultry farm odours,” Environmental Monitoring and Assessment, vol. 135, no. 1-3, pp. 399-408. 6. L. Pan, and S.X. Yang (Submitted). “A Neuro-dynamics Model for Odour Dispersion around Livestock Farms,” Computational Intelligence and Applications, submitted in 2011. 101 Conference Papers 1. L. Pan, S.X. Yang, G.S. Mittal, S. Gregori, F. Wang, (2010). “A neurodynamics model for odour dispersion around livestock farms,” In Proc. IEEE International Conference on Systems Man and Cybernetics, pp.843-848. 2. R. Liu; L. Pan; S.X.Yang, M.Q.-H. Meng, (2008). “A web based collaborative portal for remote monitoring and analysis of livestock farm Odour,” In Proc. IEEE/ASME International Conference on Advanced Intelligent Mechatronics, pp. 371-376. 3. L. Pan, R. Liu, S. Peng, S.X. Yang, and S. Gregori, (2007). “Real-time monitoring system for odours around livestock farms,” In Proc. IEEE International Conference on Networking, Sensing and Control, pp. 883-888. 4. L. Pan, R. Liu, S. Peng, Y. Chai and S.X. Yang, (2007). “An wireless electronic nose network for odours around livestock farms,” In Proc. 14th International Conference on Mechatronics and Machine Vision in Practice, pp. 211-216. 5. L. Pan and S.X. Yang (2007). “Analyzing livestock farm dour using a Neurofuzzy approach,” In Proc. International Conference on Intelligent Computing, pp. 772-777. 6. R. Charles, Y. Krupin, J. Holstead, A. Trcka, L. Pan, and S.X. Yang, (2007). “Development of a new electronic nose for odour measurement utilizing wireless sensor networks,” In Proc. International Conference on Networking, Sensing and Control , pp. 455-459. 102 IEEE/ASME TRANSACTIONS ON MECHATRONICS, VOL. 14, NO. 3, JUNE 2009 371 Short Papers An Electronic Nose Network System for Online Monitoring of Livestock Farm Odors Leilei Pan and Simon X. Yang Abstract—An electronic nose (e-nose)-based network system is developed for monitoring odors in and around livestock farms remotely. This network is built from compact e-noses that are tailored to measure odor compounds and environmental conditions such as temperature, wind speed, and humidity. The e-noses are placed at various applicable locations in and around the farm, and the collected odor data are transmitted via wireless network to a computer server, where the data processing algorithms process and analyze the data. The developed e-nose network system enables more effective odor management capabilities for more efficient operation of odor control practice by providing consistent, comprehensive, real-time data about the environment and odor profile in and around the livestock farms. Experimental and simulation results demonstrate the effectiveness of the developed system. Index Terms—Electronic nose (e-nose), odor dispersion, odor monitoring, wireless sensor network. I. INTRODUCTION Odor emissions from livestock operations have caused significant public concerns about the environment and human health. Odor control technologies are greatly impeded by the lack of science-based approaches to assess the effects of odor. With the current state of technology, the most commonly used methods to measure odor from livestock facilities is human sniffing methods. Odor measurement technologies such as olfactometry and scentometer use trained odor panelists to smell air samples to determine the strength of an odor [1]. Both olfactometry and scentometer methods are expensive to use and are susceptible to the assessor’s personal bias. Therefore, unbiased automatic odor evaluating technologies are desirable. An electronic nose (e-nose) is such a reliable alternative for objectively measuring livestock farm odors. Now, e-noses have been developed for monitoring agricultural environment. In our previous study, an e-nose consisting of a sensor array and an intelligent analysis system was developed. Experimental results proved its capability of producing objective and consistent odor measurement [2]. However, an e-nose is not enough to provide a comprehensive odor profile in and around the livestock facility. The impact area of odorous gas emissions is difficult to determine because it continuously changes with wind speed and direction. In addition, a modern livestock facility is usually not a point odor source but rather an array of points (exhaust fans), lines (side curtain opening), and areas (outdoor storage surfaces). An e-nose can only measure the odor at one location; it cannot provide an overall odor profile around livestock facilities that is necessary for a comprehensive and efficient odor management strategy. Manuscript received June 10, 2008; revised September 20, 2008. First published March 10, 2009; current version published June 17, 2009. Recommended by Technical Editor H. Hashimoto. This work was supported in part by Ontario Pork and in part by the Natural Sciences and Engineering Research Council of Canada (NSERC). The authors are with the School of Engineering, University of Guelph, Guelph, ON N1G 2W1, Canada (e-mail: [email protected]; syang@ uoguelph.ca). Color versions of one or more of the figures in this paper are available online at http://ieeexplore.ieee.org. Digital Object Identifier 10.1109/TMECH.2009.2012850 Wireless communication technology has been widely applied to environmental observations [4], [9], habitat monitoring [3], medical diagnostic [10], [11], and various industries [12], [14]. Wireless network enables remote data collection, and can provide localized measurements and detailed information that is hard to obtain through traditional instrumentation [4]. Data analysis and display of the measured data and analysis results in real time are an important issue in mechatronic system development. Many end users of the system such as livestock farmers do not have sufficient knowledge of the complicated system and computer skill to program the data analysis; therefore, user-friendly interface is desirable [2]. In this research, a novel wireless e-nose network system is developed for online monitoring of livestock farm odors. The system utilizes an e-nose network built from compact e-noses that can measure odor compounds and environmental conditions such as temperature and humidity. The e-noses are placed at various applicable locations in and around the farm; odor data measured by the e-noses are delivered via a wireless network to the analysis station where several data analysis algorithms process and analyze the data, display sensor signals in real time, and model the odor dispersion around the farm. A software suite is also developed to perform all the data analysis and support users’ odor control practice. A novel intelligent odor dispersion model is proposed for modeling the odor dispersion downwind of odor sources. By using the wireless e-nose network system, real-time downwind odor measurement becomes possible. The proposed real-time odor monitoring system would enable the remote measuring and location of livestock farm odor plume. Experimental results show that the wireless e-nose network is effective in remote monitoring and real-time analysis of livestock farm odors. Computer simulations also demonstrate that the proposed odor dispersion model is feasible in modeling odors around livestock facilities. In this paper, the design of the entire e-nose network is presented. Section II first introduces the architecture of the proposed wireless enose network system. The development of each single e-nose is given in details in Section II-A; Section II-B presents the setup of the wireless network; several data processing and analysis approaches are covered in Section II-C. Section II-D introduces the software suite developed for the e-nose network system. Several advantages of the proposed e-nose network system are finally addressed in Section III. II. DEVELOPED WIRELESS ELECTRONIC NOSE NETWORK SYSTEM In this study, an e-nose network system is developed for remotely monitoring and analyzing livestock farm odors in real time. The development of the proposed wireless e-nose network system includes following parts: the design of several simplified e-noses for measuring odor components and environmental conditions, the network architecture design, the design of a software suite to process and analyze the odor data, the development of data processing and analysis algorithms, and the integration of the whole network system. The architecture of the developed wireless e-nose network system is illustrated in Fig. 1. Essentially, the wireless e-nose network is a simple STAR network. STAR is a common network topology, with all the sensor nodes communicating with a central control station (also called sink or gateway). The wireless e-nose network system consists of various hardware components. In this section, the architecture of the wireless e-nose 1083-4435/$25.00 © 2009 IEEE 372 IEEE/ASME TRANSACTIONS ON MECHATRONICS, VOL. 14, NO. 3, JUNE 2009 Fig. 2. Fig. 1. Developed sensor array. Architecture of the developed wireless e-nose network. TABLE I SENSORS USED IN THE DEVELOPMENT OF THE E-NOSE Fig. 3. Developed e-nose consists of the sensor array inside a chamber, a data acquisition board, a mote processor, and a power supply. network and the development of essential hardware components are presented. A. Development of the Electronic Noses To be utilized in the wireless network, the e-noses should be compact in size, energy efficient, and of low cost. The design toward the final enose for the wireless sensor network includes several steps, such as the selection of sensors to measure odorous compounds most prevalent in livestock odor, the incorporation of these sensors into a sensory array, the design of a chamber to regulate gases toward the sensors, and the acquisition of the sensor array data for wireless transfer. 1) Sensor Selection and Sensor Array: Each e-nose in the network has a sensor array with four gas sensors to measure odor components in ambient air, and two sensors to measure the environmental conditions, i.e., a humidity sensor and a temperature sensor. The gas sensors are selected based on previous extensive investigation and experiments, as well as various sensors available for odor compound measurement. These four sensors can measure most of the major gas compounds in livestock farm odors. A section of this sensor list is shown in Table I. The selected sensors are fixed in an array. A developed sensor array is shown in Fig. 2. 2) Design of the Chamber: A chamber is designed to accommodate the sensor array and regulate the flow of odorous air. The sensor array is placed within this chamber, and the odorous air is directed toward the array. Since the sensors have specific requirements on the airflow rate for proper operation, a pump is used to control the airflow speed. The chamber also contains a simple diffuser to disperse the incoming odorous air or filtered clean air onto the sensor array. The sensor array faces directly into the coming air so that the surface area of the array that is exposed to the odorous air is maximized. This ensures best measuring results and minimizes the possibility of attaining improper readings. The chamber also has an input valve that is switched between odorous and carbon filtered air ports, so that the filtered odorous air can refresh the chamber and sensor when necessary. 3) Integration of the Electronic Nose: The analog sensor signals collected by the e-nose are converted to 12-bit digital signals by a data acquisition board (MDA300) right at the e-nose side. The multifunction data acquisition board MDA300 can convert up to 11 channels of analog inputs. The input analog signals range from 0 to 2.5 V, and the output digital signals range from 0 to 4095. The digital signals are delivered by a 2.4-GHz mote (MPR2400) to a gateway. Gas sensors and the air pump are powered by a 12-V dc power supply. A developed e-nose system is shown in Fig. 3. These small battery-powered e-noses are placed in locations of interest around livestock facilities to collect odor data. The e-nose network prototype consists of three e-noses at this time, which can be easily expanded to accommodate more e-noses when necessary. Usually, if an e-nose fails, other e-noses that cover the area can also provide the missing information. For instance, if e-noses A, B, and C all provide coverage of area G, when nose A fails, noses B and C still provide information on area G. Therefore, the more e-noses in a certain area, the more robust the wireless network system is to the sensor failure. However, larger density of e-noses costs more, and large redundant information could make wireless transmission slow. IEEE/ASME TRANSACTIONS ON MECHATRONICS, VOL. 14, NO. 3, JUNE 2009 373 where n is number of data points in the filter, k is the kth data point, and x̄k is a simple moving average filter given by x̄k = Fig. 4. Block diagram of the wireless network. B. Electronic Nose Network The odor data collected by the e-noses located in and around the farm are delivered by a 2.4-GHz wireless LAN to the gateway. The gateway gathers the sensor signals and inputs them into the control center via Ethernet. The block diagram of the wireless network is shown in Fig. 4. For the purposes of wireless transmission of sensor signals, in this research, the MicaZ wireless network platform from Crossbow (Crossbow Technology, Inc., USA) is adopted. This wireless network platform has advantages of the small physical size, low cost, and low power consumption, making it ideal for this odor monitoring application. The small, low-power radio and processor board MPR2400 includes a processor, radio, and battery. Power can be provided by any 3-V power source, typically two AA batteries. The radio on the MPR module consists of a basic 2.4-GHz industrial, scientific and medical (ISM) band transceiver that is compliant to IEEE 802.15.4/ZigBee protocol, an antenna, and collection of discrete components to configure the physical layer characteristics, such as signal strength and sensitivity. The gateway MIB600 provides Ethernet connectivity for communication, and allows remote access to the e-nose network data via Transmission Control Protocol (TCP)/IP. The wireless communication ability not only enables sensor data and status information to be communicated across the network, but also enables remote control of network nodes. C. Data Processing and Analysis Odor data collected by the e-noses need to be processed and analyzed to identify rules and patterns in the data. Several signal processing algorithms and pattern analysis techniques are used to analyze and process sensor data. 1) Noise Reduction: Sensor signals generated by the e-nose usually are contaminated by white noise. Noise reduction is the process of removing noise from a signal. There are many noise reduction methods to remove or reduce noise from sensor signals, such as commonly used fast Fourier transforms, Kalman filters, and moving average filters. In this research, moving average technology is adopted to reduce noises in signals as it is fast in computing and easy to use in comparison to other existing technologies. An exponentially weighted moving average filter is used to minimize the noise [18]. The filter is given as x̄k = n x̄k −1 + n+1 n xk n+1 k xi . (1) (2) i = k −n + 1 This method applies weighting factors to the data points in the way that the weights for earlier data decrease exponentially, thus giving much more importance to recent observations while still not discarding previous observations entirely. 2) Compensation for Signal Drift: Sensor signals are usually subjected to temporal variation, typically referred to as “long-term drift,” due to temperature and humidity changes in the environment. This drift may seriously influence calibration; therefore, drift compensation approaches are used to reduce the drift. These approaches include mathematic methods based on curve fitting of the temporal variation of the sensor signals, wavelet analysis, or statistic methods such as principal components analysis. In this study, a simple data preprocessing algorithm is used to compensate the signal drift. This method compensates the variation of the conductance of the sensors that are caused by environmental changes, and therefore reduces the variation of the output electrical signals of the sensors. The general expression of the compensation approach is given as ri j = Gi j − G0i G0i (3) where G0i is the conductance of the sensor i in clean air (i.e., baseline signal) and Gi j is the conductance of the sensor i in the presence of odor j [8]. 3) Modeling of Odor Dispersion: Accurately measuring odors in and around the livestock farms has been an elusive goal of researchers and livestock producers. Air dispersion models have been applied to evaluate odor impacts around the livestock facilities and determine the setback distance between the livestock facilities and local residents [6]. Various types of dispersion models have been developed to represent different types of emission release scenarios. Commonly used dispersion models for livestock farm odor dispersion include numerical advection–diffusion models, Gaussian models, and Lagrangian particle trajectory theory. Gaussian dispersion models are used for steady-state meteorology conditions, and therefore are not suitable for modeling dynamic odor dispersion on livestock farms [15]. Numerical approaches and Lagrangian particle trajectory approaches are complicated, and require the high computational and memory capacity, and therefore are not ideal for online odor analysis [5], [16]. As livestock farms have special odor emissions, farmsteads, and odor management systems, an ad hoc livestock farm odor dispersion model can greatly improve the efficiency and accuracy of odor dispersion prediction. A neural network model is proposed for modeling dynamic odor dispersion around livestock farms. The neural dynamics of each neuron is characterized by a shunting equation of an additive equation derived from Hodgkin and Huxleys’s membrane equation [7]. A 2-D dynamic odor dispersion model is proposed as dC(Xi , t) = −AC(Xi , t) + (B − C(Xi , t)) dt ([I(Xi , t)]+ + N wi j [C(Xj , t)]+ ) j=1 − (D + C(Xi , t))[I(Xi , t)]− 1− 1 n (4) where Xi = (x, y) is the location of the ith neuron, i = 1, 2, . . . , N , N is the total number of neighboring neurons of the ith neuron, x is the 374 IEEE/ASME TRANSACTIONS ON MECHATRONICS, VOL. 14, NO. 3, JUNE 2009 downwind distance of the ith neuron to the odor source, while y is the crosswind distance of the ith neuron to the odor source, C(Xi , t) is the odor concentration at the ith neuron, and [I(Xi , t)]+ and [I(Xi , t)]− are excitatory and inhibitory inputs, respectively, where [e]+ and [e]− are linear-above-threshold functions defined as [e]+ = max {e, 0} [e]− = max {−e, 0} . (5) Parameters A, B, and D represent the passive decay rate, the upper and lower bounds of the neural activity, respectively. Parameters B and D are constants, and A can be a function of wind speed, i.e., A = qW (t) (6) where q is a positive constants and W (t) represents the wind speed at time t. Therefore, parameter A represents the transportation effect of airflow; the higher the airflow speed, the faster the odorous compounds being dispersed. The variable I(Xi , t) is external input to the ith neuron defined as I(Xi , t) = ⎧ S(t), ⎪ ⎪ ⎪ ⎪ ⎨ −R(t), ⎪ ⎪ ⎪ ⎪ ⎩0, if there is an odor source at position Xi if there is an odor reduction means (7) at position Xi if there is no odor source or reduction means at position Xi where S(t) and R(t) are positive functions of time. The odor reduction means include odor control practice operated by livestock producers, dry deposition to the earth surface, and wet deposition by precipitation (rain, fog, snow, etc.). N In (4), item j = 1 wi j [C(Xj , t)]+ represents the interaction among the neighbor neurons, where N is the total number of neighboring neurons of the ith neuron and wi j is the connection weight from jth neuron to the ith neuron, which is a function of distance defined as wi j = 0, u , di j if di j > r0 if di j ≤ r0 (8) where r0 is a positive constant and di j = |Xi − Xj | represents the Euclidean distance between positions Xi and Xj in the state space. Therefore, the neuron has only local lateral connections in a small region (0, r0 ). Function u is a function of wind direction, which is defined as θ (9) u(θ) = −E cos 2 where θ is the angle between the wind direction and the position of the neighbor neuron. When 0 ≤ θ ≤ 90, the neighbor position Xj is downwind of Xi , the position being inspected; therefore, odor emissions are transported from position Xi to Xj , which causes a depletion of odor concentration/intensity at position Xi ; when 90 < θ ≤ 180, the neighbor position Xj is upwind of Xi ; therefore, odor emissions are transported from position Xj to Xi , which results in a replenishment of odor concentration/intensity at position Xj ; when θ = 180 or θ = 0, the position Xj is right on the centerline of the wind direction; therefore, the odor concentration/intensity at Xi yields a maximum replenishment/depletion effect. The emission rate from the odor source, the environment conditions (such as wind direction and speed), and farming activities (such as spreading manure, applying odor reduction means) may vary with time. The activity landscape of the neural network dynamically changes due to the varying external inputs and the internal neural connections, and therefore dynamically represents the changes of odor dispersion. Fig. 5. Calculated odor dispersion from a point source. (a) Odor plume profile. (b) Odor downwind along plume centerline. (c) Odor along crosswind direction. An example of calculated odor dispersion from a point source is shown in Fig. 5. The 2-D odor dispersion at receptor’s height is shown in Fig. 5(a), downwind along plume centerline in Fig. 5(b), and along crosswind direction in Fig. 5(c). Previous research on odor dispersion indicated that the location of the e-noses would influence the measurement results and the odor dispersion model. The odor dispersion plume is usually affected by the landscape around the farm such as vegetation and buildings. For example, high crops in the field serve as windbreaks, which would greatly reduce the odor dispersion [15]. A building could cause a downwash effect, e.g., airflow forms a “cavity” with recirculating flow downwind of the building, which would greatly change the odor dispersion close to that building [17]. Therefore, it is important to make sure that the odor information around these locations is available by deploying sensor nodes to these locations. D. Software Integration In order to utilize the previously mentioned hardware, certain software packages are needed. The MDA300, the MICAz, and the MIB600 all run on TinyOS programming language. An application provided with the hardware called MoteWorks is sufficient to set up a wireless network for data transfer. This involved placing the gateway (MIB600) in the same subnet as the client station and using a simple hub to successfully place the device onto the network. Then, MoteWorks autoconfigures and acquires the IP address of the network. Once the gateway device is active, the mote is programmed to interface with the e-noses. Then, the wireless network system can be integrated and synchronized. An application software called MoteView from Crossbow (Crossbow Technology, Inc., USA) can automatically detect the e-nose and the gateway, and receive data from the e-nose through the wireless network. The topology of the wireless network interface is shown in Fig. 6. The odor data collected by the e-nose network need to be processed and analyzed for the purpose of supporting odor control decisions. Hence, software suite is designed for real-time data capture, display, and analysis. The software is running on a central analysis station, and includes four main components: 1) a data acquisition system to IEEE/ASME TRANSACTIONS ON MECHATRONICS, VOL. 14, NO. 3, JUNE 2009 Fig. 6. 375 Fig. 7. Dynamic display console. Fig. 8. Odor analysis system. Topology of the wireless network. acquire the sensor signals that are transferred to the computer via the wireless network; 2) a database that stores the odor data for future applications; 3) an odor analysis system that identifies risk factors of odor and calculates odor concentration and odor dispersion plume; and 4) a user interface tire that interacts with users and displays odor data and reports analysis results. 1) Data Acquisition System: After analog sensor signals are converted into digital signals, the digital signals are sent wirelessly through the wireless network to the gateway, which receives and collect the digital signals. Then, the data acquisition system in the computer will interface with the gateway and acquire the digital signals into the database. 2) Database: Database plays an important role as a data storage, management, and exchange center for various applications. In this system, data structures (data records and files) are optimized to deal with large amounts of data. A data storage system based on Microsoft SQL Server 2005 database server is developed for storing sensor signals and odor information. The stored odor data in the database can be retrieved, managed, and processed through user interface. 3) User Interface Tire: Users interact with the control station directly through a series of user interfaces. These interfaces are within the user interface layer. The user interfaces provide a user-friendly environment and adequately support the operation undertaking. The user interface layer provides graphical front-end pages to the database records, analysis result reports, task management, and the other components. Microsoft C# environment is used for user interface development. The main interface is the first displayed page when users start the application. Users log onto the system from this page to get the access to other functional pages such as data display interface and analysis system interface. To satisfy various requirements, the user interface layer can provide a widely used standard for analyzing record data and also accommodates applications developed in other programming language environment. The interface of data display system is shown in Fig. 7. This system provides online and offline display of the sensor signals. Several display attributes can be selected by users, such as different update interval, number of visible points and data range, average number display, etc. This system would be helpful for routine operation and various studies. 4) Odor Analysis System: Odor analysis system is the key component of the software suite. The system utilizes the data analysis applications to identify rules and patterns in the data, to tell the users where is odor, how strong the odor is, to give suggestion on odor control practice, and to predict the resulted odor strength if any odor control means are applied. The data analysis system has many subsystems: odor strength analysis system calculates odor concentration/intensity using artificial intelligent techniques; odor dispersion analysis system calculates odor dispersion plume using odor dispersion models based on odor emission rates, and topological and meteorological information; risk factor analysis module identifies significant risk factors that cause odor problems; and decision-making module assists users to make odor control decisions based on analysis results and human expert knowledge. The interface of odor analysis system is shown in Fig. 8. This analysis system also provides decision support for farmer’s odor control practice. In the analysis system, the sensor fusion algorithm and odor dispersion algorithm calculate the odor dispersion, display the odor plume dispersion in real time, and predict the odor map if any environmental factors are changed or different control means are applied. Based on odor plume prediction results, the analysis system would provide suggestions on possible odor control practice based on farmstead, topography, and meteorological data, such as when to spread manure, change vent direction, and where to plant trees to block odor. The odor dispersion analysis system provides an overall odor mapping around live facilities that is necessary for an overall odor management strategy. This will help the livestock producers to make strategic funding decisions by investing only on efficient odor control practice. To validate the developed hardware and software system, a series of experimental studies is conducted in laboratory using odorous gases with single or multiple odor components. The results show that the developed system is functional and effective in remote data collection, wireless data transmission, and real-time display and analysis of odor data. Simulation studies on the odor dispersion model show its feasibility to model the dynamic odor dispersion around livestock farms. 376 IEEE/ASME TRANSACTIONS ON MECHATRONICS, VOL. 14, NO. 3, JUNE 2009 III. DISCUSSIONS The proposed e-nose-based wireless network approach to automatically monitor livestock farm odors differs from any existing e-noses in the following ways. 1) The developed wireless e-nose network system can provide multilocation odor monitoring while existing e-noses can only provide odor monitoring at a single location. Odor concentration changes with source configuration (shape of the source, building geometry, etc.), landscape around the farm, and distance to the source. The proposed wireless e-nose network system can provide a more comprehensive odor plume profile than a single e-nose system. 2) By using this wireless e-nose network system, users can monitor the odor from a remote location such as inside a house or office, so that they do not have to stay in the field, which makes odor measurement easier in unfavorable weather. 3) The developed e-nose network system with multiple e-noses is more robust to sensor failure and noise. One or more failed or incorrect sensor reading could be corrected through the redundant information from the other e-noses. The developed wireless e-nose network system is a useful tool in remote monitoring of livestock farm odor, which will greatly improve the efficiency of livestock farm odor reduction, and thus benefit livestock industry. In addition, it would be used for prediction of upcoming odor plume profile with weather information forecast, and planning upcoming odor management. IV. CONCLUSION The design of an e-nose-network-based livestock farm odor monitoring system was presented in this paper. The system is composed of e-noses that can measure odor compounds and environmental factors. The e-noses are deployed in and around the farm and odor data are collected via wireless communication. The data processing algorithms and odor dispersion algorithm in the analysis system calculate the odor dispersion, display the odor plume dispersion in real time, and can also predict upcoming odor plume with upcoming weather forecast. The main contribution of this research include the following: 1) an e-nose-based wireless network is developed for monitoring livestock farm odors; 2) data acquisition and data processing software is developed for collecting and processing odor data from the wireless e-nose network; 3) a neural-network-based odor dispersion model is proposed for modeling odor dispersion downwind of the odor source in real time; and 4) a data analysis software with user-friendly interface is developed for assisting end users in making efficient odor management decisions. The proposed e-nose network approach can automatically monitor livestock farm odors, and have many potential industrial applications. The developed wireless e-nose network system can perform remote multipoint odor monitoring, and the integrated software suite provides data analysis capabilities that would assist users’ decision making on odor control practice. 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Yang* School of Engineering, University of Guelph, Guelph, Ontario N1G 2W1, Canada Received 15 October 2008; revised 12 March 2009; accepted 1 April 2009; published online 10 June 2009 ABSTRACT. Monitoring and analysis of livestock farm environments require collection and management of large amount of data from distributed farms. There is an increasing demand for collaboration among livestock producers, environment agencies and governments. This paper presents a collaborative system for monitoring and analyzing livestock farm odours remotely, and for enhancing the collaboration among users. This system utilizes a web-based portal application as the infrastructure for running distributed applications. Livestock farm odour information is stored in central servers. Distributed users can access the data remotely, submit odour data for analysis, receive analysis results through the Internet, exchange information, and discuss odour related topics on public forums. This collaborative system provides a collaborative, robust, and user-friendly environment for distributed users to efficiently manage and process the data records, share the analysis results and other information. Keywords: collaborative web-based application, livestock farm odour, remote monitoring and analysis 1. Introduction Odour emissions from livestock facilities have caused serious complaints from rural residents. The Ontario governments have worked on setting up regulations and policies to limit odorous air emissions from livestock farms. Environment agencies and researchers have made significant efforts to investigate and resolve the livestock farm odour problems (Janes et al., 2004; Pan et al., 2007; Pan and Yang, 2007a, 2009). In order to efficiently resolve the livestock farm odour problem, there is an increasing demand for collaboration among livestock producers, consulting agencies and governments (federal, provincial and local). Through collaboration, they can share and make better use of their professional skills and expertise to serve the odour control problem. However, these users have different backgrounds, are responsible for different tasks based on their roles, and work at different locations. It is difficult to bring them together to achieve prompt and convenient information exchange and communications. High speed Internet provides a way for remote communications and data sharing. However, traditional HTML-based content only provides single public-facing web site. It is enough for small organizations to advertise their services or products, but it cannot satisfy the collaboration requirements of such a large-scaled service, management, industrial and research community. * Corresponding author. Tel.: +1 519 8244120; fax: +1 519 8360227. E-mail address: [email protected] (S. X. Yang). ISSN: 1726-2135 print/1684-8799 online © 2009 ISEIS All rights reserved. doi:10.3808/jei.200900142 By using the traditional HTML-based web technologies, it requires hundreds or even thousands web sites for all the users and activities in this odour monitoring and analysis task. It is expensive and time consuming to keep up with the need to create and manage those web sites. Due to the rapid development of platform-independent and central processing technologies, web-based applications are emerging to provide collaboration among distributed users. In such a web-based application, the essential software and database are located on a central server and are accessible over networks. Therefore, users can upload and process their odour data through Internet web browser. Many web-based applications have been developed in various areas, such as education (Chang et al., 2003; Jackson, 2003; Wang et al., 2004), information technology (Yu et al., 2003; Taguchi and Tokuda, 2005), urban planning (Counsell, 2004; Babar and Gorton, 2004), and environment monitoring (Zeng et al., 2007; Athanasiadia and Mitkas, 2007; Carswell et al., 2008). Yu et al. (2003) developed a web-based application for 3D visualization and collaboration to enable production, dissemination and use of 3D imagery and geospatial information on a hierarchical level. Counsell (2004) developed a web-based application for collaboration on managing and maintaining large area urban modeling. Taguchi and Tokuda (2005) designed a web application generator to generate data-intensive web applications by using diagrams that represent data-flow relationships among web components. Maglogiannis et al. (2006) presented a collaborative web-based application for medical diagnosis over the Internet via peer-to-peer distribution of electronic health records. Yu and Cheng (2007) developed a web-based decision support system for slopeland hazard warning, which combines with GIS 75 R. Liu et al. / Journal of Environmental Informatics 13(2) 75-85 (2009) technology and can provide real-time monitoring of rainfall via Internet Explorer. Despite broad applications of web collaboration technologies, none of them were developed for collaboration and cooperation among livestock producers and environment organizations. Zeng et al. (2007) proposed a web-based system for managing the information of urban water resource social renewability. An artificial neural network was developed for analysis of the information and the evaluation results were published via the Internet. Carswell et al. (2008) introduced a web-based mobile system for monitoring fish species at risk. The fish data is stored in a central database and is delivered to GPS-enabled hand-held devices so that professionals can access the data remotely and make decision immediately. To date, however, the information exchange among livestock farmers, environment agencies and governments on resolving odour issues is still quite limited. The objective of this study is to develop a web-based collaborative framework to assist the collaborations in resolving the livestock farm odour problems. Collaboration, in dictionary, is defined as a process that multiple people/organizations work together to achieve an intersection of common goals (Oxford, 1989). Collaborative software in information technology is an application designed for people participated in a common task to reach their goals. The proposed approach provides communication and data-sharing capability among distributed users, and thus enhances collaboration and cooperation among users with different backgrounds for more efficient odour management and decisionmaking. All the data collection, processing and analysis tools are developed in a web-based application, and can be accessed simultaneously by multiple users. Users can communicate with each other and share information from distributed locations. This proposed collaborative framework is built on Microsoft Framework 2.0 and Windows SharePoint system. Visual Studio 2005 is chosen as the development environment, and Microsoft SQL server 2005 is used as the data repository. 2. The Proposed Approach The proposed collaborative framework should provide essential collaboration capabilities for effective information sharing, data processing and management. This framework is developed for collecting, monitoring, processing, and analyzing livestock farm odour data. This solution not only provides robust data management and data integration for the odour research community, but also brings added values to the experimental data. However, the development is complex. The following aspects are considered: (1) Multiple user collaboration: In general, the collaborative framework should be able to serve multiple purposes, such as user collaboration, data processing, and information sharing. The odour data that generated by a client acquisition system and data analysis results are shared by governments and authorized agencies. Farmers can select environment agencies to analyze their odour data, and grant necessary permissions to chosen agencies to access their data. Farmers also have access to the analysis results and feedbacks from agencies. Ag- 76 encies process and analyze the data, generate result reports, provide suggestions on odour control practice. Governments review the odour data and analysis results, provide guidance for farm operations, and enforce environmental regulations. Governments also consult with agencies to revise or improve environmental laws or regulations. Users can interact with each other either by correspondence via private emails, or by discussions threads on the public discussion board. The proposed collaborative framework provides a disciplined approach to facilitate the collaboration among livestock farmers, agencies and governments. (2) Central data management and security requirement: Generally, odour data are collected from separate farms and stored in distributed computers. In order to analyze those distributed data and generate results, the data records need to be sent into a central data server, where the data analysis system can process numerous users’ requests simultaneously. However, in this way security issue is arisen, due to the sensitive and private nature of the odour information. In order to solve the security problem, separate data record libraries are established on the central data server, and are assigned to different users. Security settings and policies are specified for sensitive information. The collaborative framework authorizes different access privilege to different users, based on their roles and responsibilities. Only authorized users have access to the content in the record library via role-based permission control; unauthorized users have no access to private contents unless the owner of the record library grants them appropriate access privilege. For example, livestock farmers do not have access to the data of other farmers unless they are granted access. This feature enhances the data security. (3) User-friendly interface: The user interface of the system provides a user-friendly and consistent environment for users with different backgrounds and expertise. User interface in computer science is a place for human-computer interaction. It provides input and output means for users to access the computer system. Users can control the system by keystrokes with the computer keyboard, or by movements and clicking of the computer mouse; the system presents the users with graphical, textural and auditory information. Considering most potential users of the application are not computer experts, it is very important that the system should provide a user-friendly interface, adopting all necessary functionality that the users need in operations and collaborations. This collaborative framework is a platform-independent web-based application, which can be run on different platforms, such as Windows, UNIX, and Linux. Users do not need to have the same platform on their computers. All users can access the real-time interactive collaborative system through a web browser. Moreover, all data acquisition applications are installed on the central server and have online connection to the central server, so that individual users do not have to process the data on their own computers. These considerations make the proposed web-based application very easy to use, and also provide great convenience for the system administrators to maintain and update the system, since they only need to modify or tune applications on the central server. In addition to the access by desktop personal com- R. Liu et al. / Journal of Environmental Informatics 13(2) 75-85 (2009) puters, the interface also provides support for mobile device access. (4) Transparent workflow: The collaborative framework is a workflow-driven application, supporting users to submit different tasks for processing data records. Workflows represent the operational information of a work procedure, such as how processes are structured, formalized, enforced, audited, and how they are synchronized. With the robust, customizable workflow framework, users can tell whether a process request has been created, reviewed, processed, or updated, and also receive the analyzed information. The central workflow-enabled processing functionality helps users to make effective decisions by providing all useful information in one central place. 3. Design of the Web Based Collaborative Framework The collaborative system is built on top of the wireless sensor networks that are deployed on livestock farms to collect odour data. The architecture of the collaborative system is shown in Figure 1. The system consists of three main components: client applications for wireless sensor networks; a central database server; and central application servers. 3.1. Client Applications for Wireless Sensor Networks The wireless sensor networks are at the lowest level of the collaborative system. They are deployed on livestock farms to collect odour data. Each wireless sensor network has many sensor nodes, where each node is an electronic nose (e-nose) that is built of gas sensors and environmental sensors to measure odorous compounds and temperature and relative humidity. Sensor signals are delivered to and stored on a local computer for future processing. The detailed information about the wireless sensor networks can be found in Pan and Yang (2009), and the development of electronic noses is described in details in Pan and Yang (2007a). The wireless sensor networks provide the collaborative system with remote odour monitoring ability. Client applications are developed to assist the data collection for the wireless sensor network. A client application includes three main components: a data acquisition module to acquire the sensor signals transmitted to the computer via the wireless network; a client-side database that stores the data, and a data transfer web service that exchanges information between the client-side computer and the central database server. 3.1.1. Data Acquisition Module The sensor signals collected on a farm are transmitted through the wireless network to a gateway, which receives and collects the digital signals. Then the data acquisition system in the computer interfaces with the getaway and gathers the digital signals into the client-side database. 3.1.2. Client-Side Database Database plays an important role as data storage, management, and exchange center. In this system, data structures (records and files) are optimized to deal with very large amounts of data stored on a data storage server. A database based on Microsoft SQL 2005 database Server 2005, is developed for storing sensor signals and odour information. Besides database itself, there is also a data management system to store and organize or pre-process the data. Database management system is a complex set of software modules that help the organization, storage and retrieval of data in the database. It can connect to the database, load data acquisition console, and display sensor signals dynamically. The interface of data display system is shown in Figure 2. It provides both on-line and off-line signal display options. Several display settings can be selected by users, such as different update interval, number of visible points and data range, etc. This would be very helpful for routine operation and various studies. Figure 1. Architecture of the collaborative web-based application. 3.1.3. Data Transfer Web Service The client application uses a web service to interface with central servers. The web service feeds data to or consumes data from central servers. It provides remote accessibility for the client application to integrate with the central servers. 77 R. Liu et al. / Journal of Environmental Informatics 13(2) 75-85 (2009) Figure 2. The dynamic monitoring console. 3.2. Central Database Server The odour data collected by the wireless sensor networks from farm terminals are shared among decision makers, such as governments and environment agencies. The central database server provides data/information storage for the collaborative system. The proposed web-based application uses the central SQL Server 2005 as central data repository. SQL Server 2005 provides enhanced safety and security, high performance and scalability, as well as a wide range of database service tools. The central database stores the data, and provides the data to data processing services. Figure 3 shows the data structure of the central database. The central database stores different kinds of data sets and information such as real-time odour data that are collected online by the gas sensors on livestock farms, historical data records, farm information and user profiles, analysis results, and search index. With all these integrated database capabilities, the collaborative system can also help the researchers to improve data analysis and research efficiency. 3.3. Central Application Servers The architecture of the central application server is shown in Figure 4. It includes three application servers: a collaboration server, an application server, and an active directory server. The collaboration server provides a platform for data and information integration, sharing and exchange, as well as collaboration and cooperation ability among distributed end users. The application server is used to host data processing and analysis services to provide core data processing, reporting and content crawling functionality. All data processing features are shared across application domains through web services. The active directory server stores user information, and provides user authentication and authorization functionality. Livestock farmers, environment agencies, and government users are the designed users of the application server. The application server consists of two basic components: a data processing tier, and a user collaboration interface. The collaboration interface is the front-end of the application server that 78 Figure 3. Data structure of the central database server. Figure 4. Architecture of the central application server. contains all the user interfaces and directly interacts with users. Data processing service communicates with the central database server, and the user collaboration interface consumes the data processing results. All the data processing and analysis modules are located in the data processing module. Data processing service receives requests from users through the in- R. Liu et al. / Journal of Environmental Informatics 13(2) 75-85 (2009) Figure 5. Interface of the data processing services. terface, looks up information in the database, analyzes the data, processes the requests, generates analysis result reports, and then sends back result reports to presentation layer where the result reports are displayed. ledge. The detailed information about the odour dispersion analysis modules, risk factor analysis module, and decision making module can be found in Pan and Yang (2009). The report generation module generates the analysis result reports and provides report accessibility and management. (1) Data Analysis and Reporting Module The data analysis and reporting module provides a userfriendly environment and supports data processing operations. This module combines data analysis services and result reporting services. Figure 5 illustrates the framework of the data analysis service that is built as an inter-operable middle-tier of the collaborative system. The collaborative system utilizes the data analysis services to identify rules and patterns in the data, to inform the users where has odour and how strong the odour is, to give suggestion on odour control practice, and to predict the resulted odour strength if any odour control means are applied. The data analysis module has many sub-modules: odour strength analysis module calculates odour concentration/intensity using artificial intelligent techniques; odour dispersion analysis module calculates odour dispersion plume using odour dispersion models based on odour emission rates, topological and meteorological information; risk factor analysis module identifies significant risk factors that cause odour problems; and decision making module assists users to make odour control decisions based on analysis results and human expert know- Neural network methods are used in the data analysis module to explore the odour data pattern. Neural network algorithms mainly address the classification task. They can find nonlinear relationships among the input values of the predictable attribute, by creating an internal classification and regression models that are iteratively improved, based on the actual value (Haykin, 1999). Existing research demonstrates that neural networks can be successfully applied to odor data analysis (Janes et al., 2004, 2005; Pan et al., 2006; Pan and Yang, 2007b). The odour model used in the data analysis module has three layers as shown in Figure 6: the input layer, the hidden layer and the output layer. The input nodes form the first layer of the neural network, where each input node is mapped to one sensor data input channel such as temperature, humidity or odour sensor data. The second layer is the hidden layer that has hidden nodes, which receives input data from the input layer. The output layer presents the predictable attributes. In this case, our neural network has one single output attribute that is the odour strength. 79 R. Liu et al. / Journal of Environmental Informatics 13(2) 75-85 (2009) The neural network is a feed-forward network. Before using the neural network to provide prediction on odour strength value, it needs known data to tune the network, which is called the training process that is to find the best set of weights for the neural network. The weights of the network are randomly initialized at the beginning. In every training iteration, the network compares the actual value of the training case with the predicted value, and calculates the error at the outputs. Then the weights of the network are adjusted using the error back-ward propagation learning technique. The reason to choose neural network algorithms as the data analysis algorithm is that neural networks can handle complex as well as large amounts of training data efficiently. f1 x1 Gas Sensor 1 x2 Gas Sensor 2 f2 f3 E e2 / 2 (t y ) 2 / 2 (5) where t is the actual (desired) value of the output neuron for the processed the training sample, and e is the error at the output neuron. The weight adjusting equations are given as: wijnew wijold f j (1 f j ) xi e hj , (6) 2 v new v old j j (1 y ) f j e (7) y x3 Gas Sensor 3 x4 nodes, and M is the number of hidden neurons. This neural network updates the weights after a sample case is analyzed. In order to minimize the training error, the least mean square error E is used as the error function: where η is the learning rate, and ejh is the error of j-th hidden neuron given by: Odour Strength fj Gas Sensor 4 x5 e hj v j e Temperature x6 (8) Humidity fM Input Layer Hidden Layer Output Layer Figure 6. The structure of the neural network. The processing unit in the neural network is a single neuron node. Each node processes the combination of weighted sum of the input values into a single output value. The logistic function g(a) is selected as the activation function for the hidden nodes, and the tahn function φ(a) is selected as the activation function for the output node, which are defined as: g (a) ea e a ea e a (1) (a) 1 1 e a (2) Therefore, the outputs of hidden neurons and the output neuron are given as: N f j g ( wij xi ) j = 1, 2, …, M (3) i 0 M y ( v j f j ) (4) j 0 where y is the output of the neural network; fj is the output of the j-th hidden node; wij is the weight between i-th input node and the j-th hidden node; vj is the weight between the the j-th hidden node and the output neuron, N is the number of input 80 The parameters of the neural network such as learning rate η and number of hidden nodes are determined by trial and error. The value of η is usually set to a large value at the beginning stage of training and gradually decreases to avoid error oscillations. It allows the neural network to find out the optimum solution quickly. The neural network model performs predictions for the data analysis module and compares with the known data. By tuning the model, the user is able to explore, query and check the information that the analysis module provides. Once the data analysis module is set up and well tuned, the system can produce the reasonable analyzed results. This module uses the integration services between data analysis and reporting in SQL server 2005, providing predictive results in a flexible and scalable manner to the end users. Figure 7 shows an odour strength analysis interface using an artificial neural network approach. Figure 8 shows a comparison between the prediction results and the actual measured values of odour strength, where the predicted values match the actual ones very well. The actual measured odour strengths range from 0 to 60 TD (dilution to threshold), and the mean square error of the predicted odour strengths against the measured ones is 1.46. It demonstrates that the data analysis module produces accurate predictions. Figure 9 shows an example of the result report. A textual or graphic report is generated to show the patterns about the prediction. After the data analysis process is complete and an accurate result is created, the application generates userfriendly reports. The report will be displayed on the browser screen or be deployed to Microsoft Office. This reporting module allows the data analysis results to be easily delivered to users in a simple format. R. Liu et al. / Journal of Environmental Informatics 13(2) 75-85 (2009) Figure 7. Odour strength analysis module. Figure 8. A comparison between the predicted value and actual value of odour strength. (2) Workflow Management The sensor data processing requires the workflow management to manage the analyzing processes, record submission, processing data, routing, etc. This central application server implements a comprehensive workflow, by using Microsoft Workflow Foundation (WFF) technology. This technology has support for complex workflow functionality, rich user interface, long running processes and advanced multiple level custom actions. The collaborative system has the seamless supports for WFF. The solution is integrated with the user interactive functionality and is built up on it. The workflow integration feature can be used to provide custom workflow for any custom business process/application. Users in research community can collaborate on data records and manage data processing tasks through particular web forms. Workflows help users to adhere to consistent project processes. (3) Personalization and Targeting Personalization and targeting component is used to target user specific information. This component primarily reads information from existing data sources, and retrieves changes to user data in the parent data sources (i.e. Active Directory), and then synchronizes with the system user profile database. The system user profile database is used for managing user profiles. All the publishing interactions with user profiles, audiences and alerts are based on the proposed personalization and targeting component. Personalization and content targeting component are exposed as a functional web service, which 81 R. Liu et al. / Journal of Environmental Informatics 13(2) 75-85 (2009) Figure 9. Odour data analysis result report. allows users to control the audience to whom the content is targeted. Collaborative web site administrators (not necessarily domain or local administrators) can easily create these audiences. These audiences are logical groups that satisfy some filtering rules (AD attribute values or custom attributes) in user profile database. It should be noted that personalization and targeting are not the same as security and restricting access to content. Results management interface is an example of a content-specific functionality that controls the targeting of result reporting to specified audience. The personalization and targeting feature provides functionality such as display a result item (i.e. reports, announcements and etc) to authorized users only. The different audience can be identified by looking up the user roles. (4) Security All the applications and application servers within the collaborative system architecturally support Windows integrated authentication. Users are authenticated against their credentials and access rights as defined in the Active Directory server. All the applications make use of these user credentials and access rights to restrict specific content from being viewed by unauthorized users. Generally, the roles of a user (i.e. the Active Directory group that they belong to) determine the access permissions that he has on the collaborative web site. (5) User Interface Users interact with the collaborative framework system 82 directly through a series of user interfaces. These interfaces are within the web-based interface application. The interface application provides front-end pages to the data record libraries, analysis result reports, discussion forums, task management, workflow management and other components. The homepage is the first displayed page when users start the application. Users log into the system from this page to get the access to other functional pages such as data record page and result reporting page. The security model of the sub-pages is inherited from the homepage so that it only needs one time login when browsing through the web pages. Figure 10 shows the homepage of the system. Users can navigate through the web site easily and find the information and content they need. All task status can be monitored on the task status page. The task status control shows the task status of submitted requests, and the service status control shows the status of pending service requests of the current user. Status bar shows the completed percentage of the whole process. This page provides a way to track and close monitoring process status and participants. The public discussion forum page is a place for the users to discuss topics of their interest and to interact with each other. Livestock farmers can consult with agencies or governments to improve their odour control operations; agencies can post their comments and concerns, rural residents can deliver their complaints about odour issue. The interface of the discussion forum is shown in Figure 11. R. Liu et al. / Journal of Environmental Informatics 13(2) 75-85 (2009) Figure 10. Homepage of the collaborative portal. 4. Conclusions A collaborative portal framework is developed for acquisition, transmission, integration, management, processing and analysis of livestock odour data; and for providing a platform to enhance collaboration in livestock farm odour management. The user-friendly environment makes the application easy to use. The centralized data storage and analysis tools and models enable easy maintenance and improvement for the application. The authentication and authorization features provide enhanced security for the application. The web-based collaborative system is an efficient and convenient platform for collaboration among livestock producers, environment agencies, and governments. It not only brings a data integration solution to the research community but also increases the productivity of all the participants and brings significant added values to the data. The collaborative portal system eliminates the limitation on the livestock farm odour emission research by the Internet-accessible collaborative portal web site. The system delivers a collaborative interface to let users easily manage the process and share the data. The interface links researchers together based on the same interests about the experimental data, topics or projects. Researchers are no longer required to be in the same place working on the same project. The collaborative portal framework provides essential collaboration capabilities and dynamic, accurate, and robust environment information that promotes faster and more insightful decision making on livestock farm odour control problems. Acknowledgments. 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Yang,1 and Jiangping Shuai2 ∗ There is a lack of knowledge about which risk factors are more important in West Nile virus (WNV) transmission and risk magnitude. A better understanding of the risk factors is of great help in developing effective new technologies and appropriate prevention strategies for WNV infection. A contribution analysis of all risk factors in WNV infection would identify those major risk factors. Based on the identified major risk factors, measures to control WNV proliferation could be directed toward those significant risk factors, thus improving the effectiveness and efficiency in developing WNV control and prevention strategies. Neural networks have many generally accepted advantages over conventional analytical techniques, for instance, ability to automatically learn the relationship between the inputs and outputs from training data, powerful generalization ability, and capability of handling nonlinear interactions. In this article, a neural network model was developed for analysis of risk factors in WNV infection. To reveal the relative contribution of the input variables, the neural network was trained using an algorithm called structural learning with forgetting. During the learning, weak neural connections are forced to fade away while a skeletal network with strong connections emerges. The significant risk factors can be identified by analyzing this skeletal network. The proposed approach is tested with the dead bird surveillance data in Ontario, Canada. The results demonstrate the effectiveness of the proposed approach. KEY WORDS: Neural network; risk factor analysis; structural learning with forgetting; West Nile virus 1. INTRODUCTION cases of WNV human infection were reported in Ontario, Quebec, Manitoba, Saskatchewan, and Alberta in 2005.(7–9,15) Although WNV has been shown to infect many other species of animals, such as horses, cats, bats, and squirrels, it is believed that birds serve as primary reservoirs for WNV.(3,12,13) By feeding on infected birds, mosquitoes become infected, and then further spread the virus to humans and other animals through biting them.(1,3,6) Birds are considered as one of the most important factors in the WNV transmission cycle. Many researchers have used environmental factors to investigate WNV disease patterns. These environmental factors include metrology, topography, and soil characteristics.(14–19) The WNV infections are also influenced by climatic factors, such as temperature. West Nile virus (WNV) is a mosquito-transmitted disease that was first found in 1937 in the West Nile district of Uganda and arrived in New York City in 1999.(1,4,5) In Canada, the virus was first detected in 2001 in a wild bird in Ontario. In 2002, six provinces detected the virus. In 2003, the virus spread to seven provinces. In 2004 and 2005, however, only five provinces detected the virus. Confirmed School of Engineering, University of Guelph, Guelph, Ontario, N1G 2W1, Canada. 2 Public Health Agency of Canada, Guelph, Ontario, N1H 8J1, Canada. ∗ Address correspondence to Simon X. Yang, School of Engineering, University of Guelph, Guelph, Ontario NIG 2WI, Canada; tel: +1-519-824-4120; fax; +1-519-836-0227; syang@ uoguelph.ca. 1 487 C 2008 Society for Risk Analysis 0272-4332/08/0100-0487$22.00/1 488 Researchers have indicated that summer temperature would influence the transmission of WNV. It is believed that the higher the summer temperature is, the more quickly the mosquito develops from egg to adult stage.(2,16–19) However, some researchers suggested that winter temperature might also influence the infection of WNV, as in a mild winter, more mosquito eggs can survive, resulting in a higher mosquito population early in the next year, and thus spread more WNV in the coming spring and summer.(2,7,8) Further investigation and modeling are needed to determine the role of temperature, and identify if winter temperature patterns may be associated with WNV infection. However, the WNV transmission system is very complicated, as many species of animals and humans interact with each other.(1,3,5,6) The role of temperature in WNV disease spread and breakout is not adequately understood. Understanding the nature of this interaction would help develop new technologies for WNV prediction, prevention, and control. The influence analysis of temperature-related risk factors to WNV has been attracting much attention. Some researchers used degree-days as the major temperature indicator,(16,19) while many other researchers prefer to use mean monthly temperature as the temperature indicator.(17,18) The mathematical methods employed include logistic regression analysis,(18,19) principal component analysis,(17) and discriminate analysis.(16) However, these statistical methods for WNV infection have several weaknesses, for example, they require prior knowledge about the statistical distributions in the data sources, and they are unable to deal with vague and noisy data.(20) Soft computing techniques, such as artificial neural networks (ANNs), have been successfully applied to analysis of complex nonlinear systems where conventional statistical methods have difficulties. ANN approaches are particularly suited for exploratory analysis because of their capability to learn the association pattern among the inputs and outputs without any prior knowledge of the system being studied.(10) In addition, they can quickly adapt to changing situations, and are robust to inconsistent, incomplete, and noisy data. Therefore, ANN methods have great potential in interpreting the nonlinear interactions between infection of WNV virus and the environment.(10,21) Bayesian networks have been used in identifying input variables that have most significant influence to the outputs.(22,23,26) However, these Bayesian approaches require an exhaustive search over the model space for all the possible network structures. Therefore, the time requirement for the learning procedure increases super-exponentially as Pan et al. a function of the number of variables.(27,28) Thus they are not a good choice for analyzing the model structures with large number of variables. Fuzzy-logic-based methods can incorporate human experience and expert knowledge. However, they do not have learning ability; thus fuzzy systems have difficulty in analyzing complex systems without prior knowledge of the system being investigated. To overcome the limitations of fuzzy systems, adaptivenetwork-based fuzzy inference systems (ANFIS) that incorporate neuroadaptive learning techniques into the fuzzy logic models have been used for factor analysis, but their high computational cost makes them unsuitable for large data sets with complex data structure.(24,25) In order to investigate the influence of seasonal temperature on WNV infection, in this article, a neural-network-based approach that is able to extract analytical information from WNV analysis models was used. The main innovative aspect of this study relies on the capability of finding out the significant risk factors that contribute most to WNV infection rates. A few comparative studies are also presented in this article to show the effectiveness of the proposed algorithm. 2. METHODS A neural network model using a structural learning with forgetting (SLF) algorithm is proposed to model and analyze the NWV infection. The contribution of monthly temperature to infections of WNV is ranked in this study. Since the proposed SLF algorithm is a modified typical back-propagation learning (BPL) with forced forgetting of the connection weights, the basic idea of an SLF algorithm is introduced after a brief description of the typical BPL. 2.1. Data Sets and the Proposed WNV Analysis Model 2.1.1. Data Sets The data used for this study were collected through the national WNV dead bird surveillance in Ontario from 2002 to 2005. In each year, dead birds were collected and tested in 33 health units within the province. The dead birds tested include crows, jays, magpies, and ravens. In the data, information from each record includes the name of the health unit where the dead birds were collected, the dates when the infected dead birds were collected, the number of A Neural Network-Based Method for Risk Factor Analysis of West Nile Virus dead birds collected (in total of all species), and the number of confirmed infected birds (in a total of all species) from the health unit. The climatic information from 2002 to 2005 was collected from the website of Environment Canada (http://www.ec.gc.ca). The location information, such as longitude, latitude, and elevation of each health unit, was also collected from the spatial data of the Environment Canada website. There are a total of 132 records in the data available for developing and validating the model. Approximately 75% were randomly selected for training the neural network model and the remaining 25% of the total data sets were used for validating the trained neural network structure. Data sets were randomly selected by using a MATLAB built-in function “randperm(N),” which can generate a series of integral numbers ranging from 1 to N (where N is the number of data records) in random order. These randomly ordered numbers were used as the index of data records, so that the data records were also randomly ordered. Then the first 75% of data were used as training data, and the remaining 25% were used as testing data. This is equivalent to randomly selecting 75% of the data to be training data, since the data records were randomly ordered. 2.1.2. Statistical Analysis The infection number versus month is shown in Fig. 1. Infected dead birds were found from May to October during the study period (2002–2005), and the maximum numbers of infected dead birds were found in July for 2002 and 2003, and in August for 2004 and 2005. Fig. 1. Number of infected dead birds collected in Ontario versus month. 489 Fig. 2. Infection rate of dead birds collected in Ontario versus month. Merely analyzing the absolute number of positive dead birds is not enough. The total number of dead birds tested should also be considered because this total number varied greatly among health units. This study uses infection rate, which is the number of positive dead birds over the total number of dead birds tested. The infection rate versus month is shown in Fig. 2. The highest infection rates occur in August in 2002 and 2005, while in 2003 and 2004, highest infection rates happen in September. The monthly mean temperature of Ontario is shown in Fig. 3. July and August yield the highest mean temperature of the year, while the lowest temperature appears in January and the second lowest temperature appears in February in most of years. By comparing Figs. 1 and 2 with Fig. 3, we can assume that there is Fig. 3. Mean monthly temperature of Ontario. 490 Pan et al. Fig. 4. Performance of the statistical multiple regression method. a strong relationship between monthly mean temperature and infection rate of WNV. Generally, the infection number/rate increases as the temperature increases. However, both figures yield highly nonlinear characteristics. Therefore, a WNV risk model using conventional statistical algorithms may not be able to accurately identify the risks. For instance, a statistical multivariate regression method was performed to analyze the data. Standard diagnostics, such as normality of observations and regression residuals, and independence of observations were performed to ensure the goodness of fit using the method. A low-prediction accuracy (r = 0.446, p< 0.05) was obtained on WNV infection rate. The predicted results using multivariate regression are shown in Fig. 4. 2.1.3. Neural Network Architecture A multifactor neural network model for WNV infection rate is proposed in this article. Since the data were collected from May to October in each year, the weather conditions during the data collection periods are considered, that is, this model considers the monthly average temperatures of November and December of the previous year, and monthly average temperatures from January to October of the current year in which the dead bird surveillance data were collected. The location (longitude, latitude, and elevation) of the health unit where the data were collected is also considered as a factor that would influence the infection rate of dead birds, and included in the neural network model, because the location (longitude, latitude, and elevation) of the health unit may represent predominant weather conditions such as temperature, humidity, and other factors. The proposed neural network model has an input layer, one hidden layer, and an output layer. The model has N input neurons, where the inputs can be risk factors such as monthly temperatures and topology information. The output is the WNV infection rate. The neural network started with a fully connected network that had Q hidden units. Connections reflect the relation between input and output attributes.(11) The outputs at the hidden layer and the neural network output are given as ⎧ N ⎪ ⎪ ⎪ hk = g vki xi , i = 0, 1, . . . , N; k = 1, 2, . . . Q ⎪ ⎪ ⎨ i=0 (1) Q ⎪ ⎪ ⎪ ⎪ wkhk , k = 0, 1, . . . , Q, ⎪ ⎩y = f k=0 A Neural Network-Based Method for Risk Factor Analysis of West Nile Virus where y is the output of the proposed network; hk is the output of the kth hidden neuron; x i is the ith input variable; v ki and wk are the connection weights from the inputs to hidden layer and the hidden layer to output layer, respectively; N is the number of input neurons; and Q is the number of neurons in the hidden layer. A nonlinear sigmoid function is used for the activation function of the hidden layer, and a linear function is used for the output layer, which are defined as ⎧ x −x ⎨g(x) = e − e e x + e−x . (2) ⎩ f (x) = x The proposed neural network was trained using a SLF method. A comparison to a typical BPL was also conducted. 2.2. Typical BPL The typical BPL is based on minimizing squared errors,(10) whose error function E is defined as 1 2 1 e = (t − y)2 , (3) 2 2 where y is the predicted WNV infection rate, while t is the collected WNV infection rate, and e is the error at the network output. In a typical BPL, the weight changes w B k and B v ki are obtained by differentiating Equation (3) with respect to the connection weight wk and v ki ,(10) ⎧ ∂E B ⎪ ⎪ i = 0, 1, . . . , N; ⎪vki = −η ⎪ ∂vki ⎪ ⎨ = η 1 − h2k xi ekh , k = 1, 2, . . . , Q (4) ⎪ ⎪ ⎪ ∂E ⎪ ⎪ ⎩wkB = −η = ηhke, k = 0, 1, . . . , Q ∂wk E= where η is the learning rate; and ekh is the error at the kth hidden neuron, which is given as ekh = wke, k = 1, 2, . . . , Q. (5) 2.3. SLF A neural network learning algorithm called structural learning with forgetting was proposed by Ishikawa.(11) SLF is a modified typical BPL with forced forgetting of the connection weights. Unlike the typical BPL, in SLF a penalty term is added to the criterion function to force the unnecessary connection weights decreasing to small values. The criterion function in the learning with forgetting is given as Ef = E + ε 491 Q N |vki | + i=0 k=1 Q |wk| , (6) k=0 where the first term E on the right side is the squared error that is the same as the one in Equation (3) in the typical BPL, the second term is the penalty item, and ε is the forgetting rate. Similar to the typical BPL, the weight change, wk , and v ki are obtained by differentiating Equation (4) with respect to the connection weights w k and v ki , ⎧ ∂E f ⎪ vki = −η i = 0, 1, . . . , N; ⎪ ⎪ ⎪ ∂vki ⎪ ⎪ ⎪ ⎨ = v B − ηεsgn(vki ), k = 1, 2, . . . , Q ki (7) ⎪ ∂E f ⎪ ⎪ wk = −η k = 0, 1, . . . , N ⎪ ⎪ ∂wk ⎪ ⎪ ⎩ = wkB − ηεsgn(wk), B where v B ki and w k are the weight changes due to the mean square error, which are the same as those in Equation (4); and sgn(·) is the sign function. The second terms on the right-hand side of Equation (7) show that the unnecessary connection weights decrease continuously during the learning process. Therefore, redundant connections can be eliminated while only important connections remain in the resulting network. 3. RESULTS In this section, the results using the proposed SLF algorithm were analyzed. In order to demonstrate the effectiveness of the proposed approach, the skeletal neural network structure resulting from the SLF was compared with the same neural network structure trained using the typical BPL. 3.1. Analysis of Risk Factors The WNV neural network model was trained by the SLF algorithm. In neural network methodology, parameters such as the learning rate, the forgetting rate, and the number of hidden units are usually determined by trial and error.(10) Neural networks with different combinations of learning rate, forgetting rate, and number of hidden neurons were tried, the training error and generalization error for each one were computed, and the optimal parameter combination with both small training error (to avoid underfitting) and small generalization error (to avoid overfitting) was selected. A computing program to implement the 492 Pan et al. Fig. 5. Resulting structure of WNV model after trained using the typical BPL. proposed algorithm is developed using MATLAB on a computer with 2.1GHz CPU and 1GB RAM. In this study, the neural network model has 15 input variables, and there are around 100 data sets for training the network. Both the SLF and typical BPL algorithms were run many times, and the learning rate η = 0.01, the forgetting rate ε = 0.5, and the number of hidden unit Q = 8 were chosen. Therefore, there were 137 adjustable weights and bias involved in training the neural network. The structure of the WNV neural network model trained using the typical BPL is shown in Fig. 5, while the resulted structure of the WNV model trained us- Fig. 6. Resulted structure of WNV model after trained using SLF. ing the SLF method is shown in Fig. 6. The solid lines represent connection weights with absolute value greater than 1, and the dashed lines represent connection weights with absolute value smaller than 1. The strengths of the connections are represented by the line width. When the connection weights are too small (e.g., under a certain threshold value), the contribution of the input neurons to the output neurons is negligible. To clearly compare the resulting structures, a threshold θ = 0.1 was selected, for example, for a connection with absolute value less than 0.1, there was no line drawn. The resulting skeletal neural network structure is shown in Fig. 7. Investigation of the con- A Neural Network-Based Method for Risk Factor Analysis of West Nile Virus 493 Fig. 7. Resulting structure of WNV model after trained using SLF and after removing connections less than threshold θ = 0.01. nection weights enables the extraction of significant risk factors. Comparing the results from Fig. 7 with those from Fig. 5 indicated that 13 factors, latitude, longitude, elevation, monthly temperatures of previous December, current January to August and October, are relatively significant to the infection rate of WNV. The remaining two factors, monthly temperatures of previous November and current September, are relatively less important than these 13 factors. These results show that winter temperature (represented by mean monthly temperatures of December to February) does have an influence on WNV infection rate, while temperature in early spring and summer also affects the WNV infection rate. It is not surprising that the location factors (latitude, longitude, and elevation) of the place where the measurement was taken may be able to represent factors such as terrain conditions, weather conditions, and other environmental conditions. It should be noted that risk factors of WNV are not limited to the factors studied in this research. Although this neural network model only considers location factors and 12 monthly temperatures, other factors that are potentially associated with WNV prevalence, such as ecological factors, for instance, ecoregion and food source of birds, can also be included in the neural network model once the data are available. 3.2. Performance Analysis By using the neural network approach, the accuracy of the prediction in testing can reach r = 0.597 (p < 0.05), while that using statistical nonlinear analysis is r = 0.446. The prediction accuracy of testing data using the neural network approach is much higher than using the statistical nonlinear regression method. In order to analyze the performance of the SLF algorithm, the proposed neural network was trained by both the typical BPL and the SLF algorithms. The performance of using the typical BPL is shown in Fig. 8, and that of using the SLF is shown in Fig. 9. The performance is evaluated based on the prediction accuracy of the test sets. The Pearson correlation coefficient between the predicted infection rates and the experimental infection rates can reach r = 0.630 (p < 0.05) using BPL, while it is r = 0.597 (p < 0.05) by using SLF. This result shows that the neural network model that was trained by BPL provided more accurate prediction than the SLF. This is because the SLF minimizes the total criterion Ef in Equation (6) instead of the quadratic criterion E in Equation (3). However, the difference in prediction accuracy is small, and by using SLF, unnecessary connections fade away and a skeletal network emerges, which enables identification of significant risk factors in the WNV infection rate. 3.3. Validity of the Approach One additional step was taken in order to ensure the validity of our proposed approach. One additional neural network model (NN2) was developed by leaving out those identified unimportant factors. If the performance of NN2 is degraded significantly in comparison to that of NN1, which means those 494 Pan et al. Fig. 8. Performance of the typical BPL. Fig. 9. Performance of SLF. A Neural Network-Based Method for Risk Factor Analysis of West Nile Virus Table I. Performance of NN1a and NN2b Type of Neural Network NN1 (using multiple regression) NN1 (trained using BPL) NN1 (trained using SLF) NN2 (trained using BPL) 495 4. CONCLUSION AND DISCUSSION cr d MSE(10−2 ) 0.446 0.630 0.597 0.610 2.30 1.52 1.54 1.58 = NN model with inputs of longitude, latitude, elevation, and monthly temperatures from previous November to current October. b NN2 = NN model only with the input neurons of longitude, latitude, elevation, and average monthly temperatures of previous December, current January to August, and October. c r = correlation coefficient of predicted value and measured value. d MSE = mean squared error. a NN1 eliminated factors are actually important, then the proposed methods are not effective in identifying significant risk factors. The new model NN2 has the same structure as that of NN1, but with only 13 input neurons of latitude, longitude, elevation, temperatures of previous December, current January to August, and October. NN2 was trained using the typical BPL. In order to ensure that the results could be compared fairly as the attributes change, all the parameters, such as learning rate η and hidden units Q, remained the same as those of NN1, for example, η = 0.01, Q = 8. The performance of NN2 is shown in Table I. For NN2, the correlation coefficient of predicted infection rate and tested infection rate was r = 0.610 (p < 0.05), which was close to the results obtained from NN1 trained with BPL. The prediction accuracy of NN2 (with only 13 inputs: latitude, longitude, elevation, monthly mean temperatures of previous December, current January to August, and October) did not decrease significantly from that of NN1 (with all 15 inputs: latitude, longitude, elevation, monthly average temperatures of November, and December of the previous year, and monthly average temperatures from January to October of the current year), which indicated that the rest of the factors in NN1 other than these 13 inputs contributed little to the WNV infection rate. This result demonstrated that the proposed SLF approach was effective in recognizing significant risk factors associated with the WNV infection rate. It should be noted that the proposed method is just a data analysis method; the analysis result obtained is largely influenced by the quality of data, factors considered, and how the data were collected and processed. The validation of the result is subject to future investigation by conducting specified surveillance and studies on the WNV infection problem. There is a need to gain more knowledge on the risk factors and their influence on WNV activities and transmission. This knowledge may help local public health professionals to develop proper WNV risk control strategies and determine a risk control timeframe. Scientists believe that seasonal temperature plays a significant role in the emergence and resurgence of WNV, but how and by how much seasonal temperature influences the WNV infection is still not clear. In this article, a multifactor neural network model was proposed based on dead bird surveillance data. A neural network model with SLF was proposed to model the WNV infection and analyze the associated risk factors. After learning by the SLF algorithm, the relative contributions of risk factors to the WNV infection rates were discovered. Testing results demonstrated the effectiveness of the proposed approach. The finding that winter temperature also plays a role in WNV activities deserves further discussion. It is not clear that whether WNV is endemic or is an introduced epidemic, whether winter temperature influences WNV activities, there may be a possibility that WNV might be endemic in Canada, although it may also be interpreted that winter temperature may influence the habitat of migratory birds and mosquitoes. It is suggested that more specific surveillance and studies should be performed to investigate this problem. 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Learning Bayesian network structure from massive datasets: The sparse candidate algorithm. In Proceedings of the Fifteenth Conference on Uncertainty in Artificial Intelligence (pp. 206–215). Stockholm, Sweden. 28. Huang, Z. (2004). Bayesian computation for multilevel models: Some new methods with applications. Ph.D Thesis, Columbia University. New York. ARTICLE IN PRESS BIOSYSTEMS ENGINEERING 97 (2007) 387– 393 Available at www.sciencedirect.com journal homepage: www.elsevier.com/locate/issn/15375110 Research Paper: SE—Structures and Environment Analysing livestock farm odour using an adaptive neuro-fuzzy approach Leilei Pan, Simon X. Yang School of Engineering, University of Guelph, Guelph, Ont., Canada, N1G 2W1 article info In livestock farming, odour measurement and reduction are necessary for a cleaner environment, lower health risks to humans, and higher quality of livestock production. Article history: There have been many studies on modelling of livestock farm odour by analysing the Received 20 July 2006 chemical components in odorous air. It is suggested that the component analysis Accepted 14 March 2007 approaches should be extended to factors such as temperature, relative humidity, and Available online 2 July 2007 airflow speed. In this paper, a neuro-fuzzy-based method for analysing odour generation factors to the perception of livestock farm odour was proposed. The proposed approach incorporates neuro-adaptive learning techniques into fuzzy logic method. Rather than choosing the parameters associated with a given membership function by trail and error, these parameters could be tuned automatically in a systematic manner so as to adjust the membership functions of the input/output variables for optimal system performance. A multi-factor livestock farm odour model was developed, and both numeric factors and linguistic factors were considered. The proposed approach was tested with a livestock farm odour database. The results demonstrated the effectiveness of the proposed approach in comparison to a typical neural network model. & 2007 IAgrE. Published by Elsevier Ltd. All rights reserved. 1. Introduction In the livestock industry, measurement and control of offensive odours from livestock production facilities are very important because of the requirement of environmental conditions. Prediction of odour intensity is difficult due to the complexity of the livestock farm odour system and limited knowledge of the odours themselves (Zhang et al., 2002a, 2002b). Many factors influence odour generation. Research has indicated that environmental conditions such as temperature and humidity (Powers & Bastyr, 2004; Schiffman et al., 1995), type of facilities (Janes et al., 2004), and farm management practises affect odour generation (Zhang et al., 2002a, 2002b). However, how and by how much a factor influences livestock farm odour remains unclear (Schiffman et al., 1995, 2001; Lunn & Van De Vyver, 1977; Powers & Bastyr, 2004; Pan et al., 2006). Because of a poor understanding of the odour problem, existing odour control technologies are not efficient. Because of limitations in statistical modelling techniques, most previous studies on modelling livestock farm odour have concentrated on the modelling of the odour components and have not considered other potential odour generation factors, such as outdoor temperature, airflow speed, or type of facilities. In statistical methods, odour system models are not capable of incorporating non-numeric or subjective human expert knowledge, such as environmental conditions, type of livestock facilities. In addition, they are not well suited for dealing with the vague and imprecise information that is available in odour measurement and control. Corresponding author. E-mail address: [email protected] (S.X. Yang). 1537-5110/$ - see front matter & 2007 IAgrE. Published by Elsevier Ltd. All rights reserved. doi:10.1016/j.biosystemseng.2007.03.012 ARTICLE IN PRESS 388 BIOSYSTEMS ENGINEERING Nomenclature Aij p ai jth linguistic label of the ith input aij, bij, E fp i, j, p ith Sugeno-type parameter of consequence of pth rule cij parameters of the bell-shaped membership function Aij mean-squared error consequential output of the pth rule index numbers The rapid development of soft computing techniques, such as neural networks (NNs) and fuzzy logic technologies, allows the incorporation of those non-numeric contributing factors and expert knowledge into livestock farm odour models. Artificial neural networks (ANNs) have been used to predict odour strength (Janes et al., 2004; Pan et al., 2006). It is widely accepted that there are several advantages in using ANN algorithms over the conventional techniques, including the generalisation ability, the capability to handle nonlinear interactions mathematically, as well as the ability to automatically learn the relationship that exits between the inputs and outputs without any previous knowledge of the system being studied. However, ANNs are not capable of properly incorporating non-numeric or subjective human expert knowledge (Haykin, 1999; Zhang et al., 2002a, 2002b; Sohn et al., 2003). Fuzzy logic utilises fuzzy sets and fuzzy reasoning to provide a more human-like way of thinking in the computer programming (Zadeh & Bellman, 1977). Fuzzy logic allows intermediate values to be defined between conventional evaluations such as true/false and high/low (Zadeh, 1965). It can handle imprecise, ambiguous, and vague information, and can incorporate human experience and expert knowledge. Fuzzy logic has been used in a wide range of applications such as control systems, project management, non-linear system models, expert systems, forecasting, system identification and analysis (Zimmermann, 2001). However, fuzzy logic does not have learning ability, thus it is difficult to analyse complex systems without prior and accurate knowledge on the system being analysed (Jang, 1993; Castañeda-Miranda et al., 2006). To overcome the limitations of NNs and fuzzy systems, in this paper, an adaptive network-based fuzzy inference system (ANFIS) approach is proposed to predict livestock farm odour intensity. Two ANFIS models for livestock farm odours were developed; both numeric data and non-numeric data were incorporated in the odour models. Various comparative studies were also presented to demonstrate the effectiveness of the proposed method. 2. Methods In this section, an adaptive neuro-fuzzy inference system approach is proposed for analysing livestock farm odour. Two ANFIS models are developed after a brief description of the 97 (2007) 387 – 393 m n Oqp R wp wp xi mAij number of membership functions for each input number of inputs pth output of the qth layer, q ¼ 1, 2, 3, 4, 5 Pearson correlation coefficient firing strength of the pth rule ratio of the pth rule’s firing strength to the sum of all rules’ firing strength ith input membership function value of Aij livestock farm odour data used for the analysis. Studies of the ANFIS models are made in comparison to a multi-layer feedforward NN model. 2.1. Adaptive neuro-fuzzy inference systems In common fuzzy logic systems, membership functions are chosen by trail and error and are fixed once selected. Also, rule structure is essentially predetermined by the user’s interpretation of the characteristics of the variables in the model. However, in practice, these models may not perform satisfactorily due to limited knowledge of the system, and the improper selection of membership parameters (Jang et al., 1997). The proposed ANFIS approach incorporates an adaptive neural learning technique into the fuzzy logic model. The parameters are tuned automatically during the learning so the membership functions can well represent the non-linear system being studied with an optimal performance. The proposed ANFIS uses a Sugeno-type fuzzy relation (Jang, 1993; Jang et al., 1997; Karray & De Silva, 2005), where the fuzzy rules take the following form: Rule 1 : IF x1 is A11 and x2 is A21... and xn is An1 ; THEN f 1 ¼ a10 þ a11 x1 þ a21 x2 þ ::: þ a1n xn Rule 2 : IF x1 is A11 and x2 is A22... and xn is An1 ; THEN f 1 ¼ a20 þ a21 x1 þ a22 x2 þ ::: þ a2n xn .. . Rule p : IF x1 is A1m and x2 is A1m... and xn is A1m ; p p p p THEN f p ¼ a0 þ a1 x1 þ a1 x2 þ ::: þ an xn ; where xi, i ¼ 1; :::; n, is ith input linguistic variable in the antecedent part of the rule; variable Aij is the jth linguistic label (for instance, large, small, etc.) associated with xi. Function fp is the consequent output of the rule, and p ai is the Sugeno parameter. A general ANFIS model is shown in Fig. 1, which has n inputs and each input variable has m membership functions, resulting in a total of mn rules. Layer 1: Fuzzification layer In this layer, the crisp inputs xi, i ¼ 1,y,n, are fuzzified through mapping into membership functions of the linguistic labels Aij, j ¼ 1,y,m. The membership functions can be any appropriate parameterised functions. In this paper, all the ARTICLE IN PRESS BIOSYSTEMS ENGINEERING Layer 1 A11 (x1) x1 A111 A A122 A A1m (x1) AA1m2 A21 (x2) x2 Layer 2 B211 A Π Layer 4 Layer 3 w1 Π 389 97 (20 07) 38 7 – 393 N N w1 Σ w2 Layer 5 w1 f1 Σ Odour intensity w3 Π N Π N Σ A222 B A2m (x2) AB2m Anl (xn) xn Anm (xn) Bn11 A Bn22 A Π wmn−1 Σ wmn wmn wmn f mn N ABnm2 Fig. 1 – The structure of an adaptive neuro-fuzzy inference system (ANFIS) consists of a five-layer feedforward network. membership functions are bell-shaped functions described by mAij ðxi Þ ¼ 1 1 þ ðxi cij =aij Þ2bij i ¼ 1; 2; . . . ; n; ; j ¼ 1; 2; . . . ; m n, where aij, bij and cij are the parameters, n is the number of inputs, and m is the number of membership functions of each input. The bell-shaped functions vary accordingly when these parameters are changed, and thus exhibit various forms of membership functions on Aij. The output O1p of each node i in layer 1 is the membership functions of Aij O1p ¼ mAij ðxi Þ; i ¼ 1; 2; . . . ; n; j ¼ 1; 2; . . . ; m; p ¼ 1; 2; . . . ; m n, where variable O1p represents the degree to which the given xi associates with Aij. Layer 2: Rule operation layer In this layer, each rule node multiplies the incoming signals and sends the product out. The output of each node in layer 2 represents the firing strength of a rule, which is given as O2p ¼ wp ¼ n Y i¼1 mAij ðxi Þ; i cedent. Other t-norm operators can also be used as the node function in this layer. ji ¼ 1; 2; . . . ; m; Layer 3: Normalisation layer In this layer, the firing strengths of the fuzzy rules are normalised. The output of each node in this layer is the ratio of the ith rule’s firing strength to the sum of all rules’ firing strength, and is calculated based on wp O3p ¼ wp ¼ Pmn ; p¼1 wp p ¼ 1; 2; . . . ; mn . Layer 4: Consequent layer In this layer, the values of the consequent are multiplied by normalised firing strengths according to O4p ¼ wp f p ; p ¼ 1; 2; . . . ; mn . Layer 5: Aggregation layer The overall output of this layer is computed as a summation of all incoming signals: P X p wp f p wp f p ¼ P . O5 ¼ p wp p p ¼ 1; 2; . . . ; mn , where j1, j2,y,jn is a combination of m membership functions from the n input variables; thus there are a total of mn combinations, resulting in mn nodes in this layer. Each node in fact performs an AND operation within the rule ante- 2.2. Data sets The proposed ANFIS approach was tested using a livestock database. Data were collected in the fields downwind from ARTICLE IN PRESS 390 BIOSYSTEMS ENGINEERING four poultry, five dairy and six swine facilities in 2004. Outdoor temperature, wind speed, distances to the odour source, and odour dilution-to-threshold (D/T) intensity levels perceived by human assessors were recorded and at the same time, an electronic nose developed by Yang and his research team in The University of Guelph took measurement at each site (Pan & Yang, 2007). The electronic nose had 14 gas sensors that were selected for detecting the gas compounds such as hydrogen sulphide in livestock farm odour. The sensor signals from the electronic nose represent the concentrations of gas compounds in the air. The detailed measuring procedure is described in the paper by Pan et al. (2007). The data of temperature, type of facility and concentration of hydrogen sulphide were used in odour generation modelling in this study. Factors such as wind speed and distance to odour source are not considered as they mainly influence downwind transportation of odour emissions. By using a statistical multi-variant linear regression method, temperature and concentration of hydrogen sulphide, being independent variables and odour intensity being a dependent variable, a low accuracy (r ¼ 0:40, Po0:01) was obtained on prediction of odour intensity. It is not surprising that low accuracy was achieved using this method, as livestock farm odour systems are highly non-linear in character. There were a total of 64 samples in the data available for the development and testing of models. Approximately 80% of the data points were randomly selected training the NN, and the remaining 20% were used for testing. 2.3. Livestock farm models In this section, three models are developed based on the database mentioned above. The details of the three models Layer 1 Layer 2 97 (2007) 387 – 393 are introduced below, two using an ANFIS approach and the third using a NN. 2.3.1. Model 1: an ANFIS model with two inputs To estimate the odour intensity, an ANFIS model (model 1) was designed. The model has 2 inputs: temperature and concentration of hydrogen sulphide. Researchers have indicated that the concentration of hydrogen sulphide can act as an indicator for livestock farm odour strength (Lunn & Van De Vyver, 1977; Williams, 1984). The concentration of hydrogen sulphide was represented by the magnitude of the output signal of a sensor that was selected for detecting hydrogen sulphide. All the inputs are numeric and have to be fuzzified in the fuzzification layer. The output of model 1 is the odour intensity. Three membership functions are associated with each input, thus resulting in 9 IF–THEN rules in the model. Model 1 was trained by a back-propagation (BP) algorithm (Haykin, 1999). 2.3.2. Model 2: an ANFIS model with three inputs In order to test the ability of the proposed approach of incorporating linguistic information, another ANFIS model (model 2) was developed. The model has 3 inputs: temperature, type of facilities, and concentration of hydrogen sulphide. The type of facilities can generally represent such measures as type of animals, farm activity, odour emission rates, and other physical measures that may influence the odour intensity. The output is the odour intensity. Model 2 also has 3 membership functions for each input, resulting in 27 IF–THEN rules in total. Model 2 is illustrated in Fig. 2. The model was trained by BP algorithm. Layer 3 Layer 4 Π N Σ Π N Σ Layer 5 Dairy Type of facility Swine A2 Poultry Odour intensity Cold Temperature Π N Π N Π N Σ Medium 2 Hot B Low B1 H 2S Σ Medium B 2 High B2 Fig. 2 – An ANFIS model with three inputs and 27 IF–THEN rules for analysing livestock farm odour. ARTICLE IN PRESS BIOSYSTEMS ENGINEERING Input layer Hidden layer Output layer Odour Membership intensity Fig. 3 – Structure of a neural network model for analysing livestock farm odour. 3. 5 10 0 25 50 0 (b) 35 5 25 10 15 20 25 Temperature ˚C Medium 50 40 High Medium Low 1 0.8 0.6 0.4 0.2 0 30 75 100 125 150 175 200 225 250 Concentration of H2S, ppm 1 Cold . 08 0.6 0.4 0.2 0 0 15 20 25 Temperature, ˚C Medium 1 Low 0.8 0.6 0.4 0.2 0 (a) Results and analysis In order to demonstrate the effectiveness of the proposed approach, the odour intensity prediction results from both ANFIS models were compared with that from NN model. In neuro-fuzzy technology, parameters such as learning rate and training epochs, and the number of membership functions for each input are usually determined by trial and error. In this study, the sensitivity of these parameters were investigated and showed following results: (1) With each increment of the training epoch, the accuracy of odour intensity prediction improves. After 100 epochs, the curve of mean-squared error tends to stabilise. (2) The number of membership functions for each input reflects the complexity of ANFIS for tuning parameters. If the number is too small, it is not enough to approximate the complex relationship of data; if the number is too large, it produces the redundancy for the non-linear relationship of data. Three membership functions for each input variable results in highest accuracy of odour intensity prediction in this study. (3) The higher the learning rate, the faster the network converges. However, if the learning rate is too large, the network oscillates. In this study, a learning rate of 0.1 was selected by trial and error. Hot 30 35 40 High 75 100 125 150 175 200 225 250 Concentration of H2S, ppm Fig. 4 – Comparison of initial membership functions (MF) for temperature and for H2S before the learning (a) with final membership functions after learning (b) using model 1. squared error E ¼ 1:23 105 ) compared to that from model 3. The result demonstrated that the proposed ANFIS model can incorporate human experience and knowledge, which allows prior knowledge to be embedded in the model, and this improve the results of learning. The comparison of initial membership functions before the learning with final membership functions after learning using model 1 is shown in Fig. 4. The membership functions were all changed during the learning. The results show that the proposed ANFIS approach is able to tune the membership functions so as to improve performance index. 3.2. 3.1. 0 Model 3: a neural network model In order to analyse the effectiveness of the proposed approach, a feedforward NN model was designed with an input layer, one hidden layer, and an output layer. A threelayer NN is theoretically able to learn any non-linear relationship at a desired level of precision with sufficient number of hidden neurons in the network architecture and through sufficient learning. Fig. 3 illustrates the proposed network structure for livestock farm odour. The model has two input neurons, where the inputs are temperature and concentration of hydrogen sulphide. The output is the odour intensity. The NN was trained by a typical BP algorithm, and the results were compared with the results from models 1 and 2. Membership 2.3.3. Hot Medium 1 Cold 0.8 0.6 0.4 0.2 0 Temperature H2S 391 97 (20 07) 38 7 – 393 Performance of model 2 Performance of model 1 The performances of models were evaluated based on the accuracy of odour intensity prediction and mean-squared error of the predicted odour intensities. By using the proposed ANFIS approach, the odour intensity prediction accuracy was significantly improved (correlation coefficient r ¼ 0:946; mean By incorporating type of facility, the prediction accuracy was improved (r ¼ 0.976; E ¼ 1.06 1015) compared to those of models 1 and 3. The comparison between initial membership functions before the learning and final membership functions after learning using model 2 is shown in Fig. 5. The parameters of type of facilities were changed during the ARTICLE IN PRESS 392 BIOSYSTEMS ENGINEERING Membership 1 0.5 0 1 0.5 0 0 Dairy Swine Poultry Dairy Swine Facilitytype Poultry Cold Medium Hot 5 0 25 (a) Membership 1 0.5 0 15 20 25 Temperature, ˚C High Swine Poultry Dairy Swine Facility type Poultry Hot Medium 5 10 15 20 25 Temperature, ˚C Low 0 40 Dairy 0 1 0.5 0 35 50 75 100 125 150 175 200 225 250 Concentration of H2S, ppm Cold 1 0.5 0 30 Medium Low 1 0.5 0 (b) 10 25 Medium 50 30 35 40 High 75 100 125 150 175 200 225 250 Concentration of H2S, ppm Fig. 5 – Comparison of initial membership functions (MF) for temperature and for H2S before the learning (a) with final membership functions after learning (b) using model 2. learning. This result shows that type of facility does affect odour intensity. The proposed ANFIS approach is able to interpret linguistic variables such as type of facility and cloud cover conditions, and therefore increase the accuracy of odour intensity prediction. Factors such as temperature and type of facility were actually measured or known exactly before the analysis. Their data were not fuzzy. However, by fuzzifying these data and applying ANFIS, the predicted odour intensities yield higher accuracy than those by using classical or crisp methods (multi-variant linear regression or NN). By using fuzzy sets and fuzzy reasoning, the proposed method is able to break stiffness and inflexibility that lie in accurate and precise classical or crisp logic, and make fuzzy logic more ‘‘soft’’ and dexterous. 3.3. Performance of model 3 In this study, BP learning approach was run many times, a learning rate of 0.05 and 5 hidden units were selected by trail 97 (2007) 387 – 393 and error to obtain a minimum mean-squared error. The correlation coefficient of the predicted odour intensity and perceived odour intensity was 0.522 by using NN technique alone. The mean-squared error was 0.516. The predicted odour intensities were not as accurate as those of models 1 and 2. 4. Conclusion The prediction of odour strength in and around livestock facilities has been investigated by many researchers over past decades. Prediction of the odour strength is difficult due to limited knowledge on livestock farm odour itself. It is known that a livestock farm odour system is a highly non-linear system that is influenced by many odourous components and various factors such as environmental conditions and type of facilities. Statistical linear regression approaches are therefore no longer suitable for modelling livestock farm odour. The rapid development of soft computing techniques provides more opportunities for improving accuracy of livestock farm odour modelling. An adaptive neuro-fuzzy-based approach, which incorporates neuro-adaptive learning techniques into fuzzy logic method, was proposed to analyse livestock farm odour. The proposed approach can incorporate non-numeric data and subjective human expert knowledge, which allows prior knowledge to be included in the model. In addition, the membership functions can be tuned during the learning according to input/output data to optimise performance. This feature is important as it can avoid inappropriate predetermination of membership functions, thus minimising errors resulting from the limited knowledge of the livestock farm odour system. A livestock farm database collected by our research team has been used in this study. The results show that the proposed approach is effective and provides a much more accurate odour prediction in comparison with a typical multi-layer feedforward neural network. R E F E R E N C E S Castañeda-Miranda R; Ventura-Ramos E; Del Rocio Peniche-Vera R; Herrera-Ruiz G (2006). Fuzzy greenhouse climate control system based on a field programmable gate array. Biosystems Engineering, 94(2), 165–177 Haykin S (1999). Neural Networks: A Comprehensive Foundation. Prentice-Hall, Upper Saddle River, NJ Janes K R; Yang S X; Hacker R R (2004). Single component modelling of pig farm odour with statistical methods and neural networks. Biosystems Engineering, 88(3), 271–279 Jang J S R (1993). ANFIS: adaptive-network-based fuzzy inference systems. IEEE Transactions on Systems, Man, and Cybernetics, 23(3), 665–685 Jang J S R; Sun C; Mizutani E (1997). Neuro-Fuzzy and Soft Computing: A Computational Approach to Learning and Machine Intelligence. Prentice-Hall, NJ, America Karray F; De Silva C (2005). Soft Computing and Tools of Intelligent Systems Design: theory and Applications. Addison-Wesley, NJ, America Lunn F; Van De Vyver J (1977). 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Manitoba Livestock Manure Management Initiative Inc., Winnipeg, Canada Zhang Q; Yang S X; Mittal G S; Yi S J (2002b). Prediction of performance indices and optimal parameters of rough rice drying with neural networks. Biosystems Engineering, 83(3), 281–290 Zimmermann H (2001). Fuzzy Set Theory and its Applications. Kluwer Academic Publishers, Norwell, MA, USA Environ Monit Assess (2007) 135:399–408 DOI 10.1007/s10661-007-9659-5 A new intelligent electronic nose system for measuring and analysing livestock and poultry farm odours Leilei Pan · Simon X. Yang Received: 11 September 2006 / Accepted: 12 February 2007 / Published online: 24 March 2007 © Springer Science + Business Media B.V. 2007 Abstract This paper introduces a new portable intelligent electronic nose system developed especially for measuring and analysing livestock and poultry farm odours. It can be used in both laboratory and field. The sensor array of the proposed electronic nose consists of 14 gas sensors, a humidity sensor, and a temperature sensor. The gas sensors were especially selected for the main compounds from the livestock farm odours. An expert system called “Odour Expert” was developed to support researchers’ and farmers’ decision making on odour control strategies for livestock and poultry operations. “Odour Expert” utilises several advanced artificial intelligence technologies tailored to livestock and poultry farm odours. It can provide more advanced odour analysis than existing commercially available products. In addition, a rank of odour generation factors is provided, which refines the focus of odour control research. Field experiments were conducted downwind from the barns on 14 livestock and Supported by Ontario Pork, Natural Sciences and Engineering Research Council (NSERC), and Ontario Ministry of Agriculture and Food (OMAF) of Canada. L. Pan · S. X. Yang (B) School of Engineering, University of Guelph, Guelph, Ontario N1G 2W1, Canada e-mail: [email protected] poultry farms. Experimental results show that the predicted odour strengths by the electronic nose yield higher consistency in comparison to the perceived odour intensity by human panel. The “Odour Expert” is a useful tool for assisting farmers’ odour management practises. Keywords Livestock and poultry farm odours · Electronic nose · Expert system · Neural network · Artificial intelligence Introduction In livestock and poultry farming, odour measurement and reduction are necessary for a cleaner environment, lower health risks to humans, and higher quality of livestock and poultry production. However, efforts to remedy odours from livestock and poultry production facilities have been impeded by the lack of science-based approaches to assess odour control technologies and the true effects of odour (Zhang et al. 2001). Livestock and poultry farm odours are complex mixtures of hundreds of compounds that interact with each other; and the intensity of an odour is not a simple sum of intensities of all odorous compounds within it (O’Neill and Philips 1992; Schiffman et al. 2001; Zahn et al. 2001). Besides, researchers have indicated that many environmental factors such as temperature, air flow speed, and relative humidity 400 Environ Monit Assess (2007) 135:399–408 also influence odour generation (Powers and Bastyr 2004; Zahn et al. 2001). To date, the most commonly used “instrument” for measuring odour is still human nose. Trained human panels have been used in most studies in measuring odour strength and offensiveness (Zhang et al. 2001, 2002). However, human panels are expensive to use and are difficult to overcome the analyst’s personal bias and adaptation to odour in odour measurement and evaluation. Therefore, unbiased automatic odour evaluation technologies are desirable. Electronic noses are promising considering its low operational cost, consistent and objective readings. Electronic nose is an instrument which is composed of an array of electronic gas sensors and a signal-processing system, and is capable of recognising simple or complex odours (Gardner and Bartlett 1999). The output of an electronic nose can be either the identity of chemical components of the odour, or a prediction of any characteristic properties of the odour such as intensity and nuisance. Electronic nose technology has proved a useful tool in the food industry, medicine, and environmental monitoring (Barlett et al. 1997; Loutfi 2006; Shaw et al. 2000; Schaller et al. 1998; Singh et al. 1996). However, little work has been done to use electronic nose for agricultural environment monitoring (Hobbs et al. 1995; Hudon et al. 2000; Osarenren 2002; Powers and Bastyr 2004; Qu et al. 2001). No commercially available electronic noses were developed in particular for detecting livestock and poultry odours. Moreover, most electronic noses developed previously can only be used in the laboratory, in which the environmental condition is apparently different from that in field. Therefore, the accuracy and reliability of the measurement are Table 1 Partial list of the sensors used for the developed electronic nose inevitably reduced. It is highly desirable to develop a new electronic nose for measuring livestock and poultry farm odours which have great commercial potential. In this paper, a new intelligent portable electronic nose was developed especially for measuring livestock and poultry farm odours in both laboratory and field. An expert system was developed for assisting farmer’s odour management operations. In order to test the developed electronic nose and expert system, field experiments were conducted downwind of 14 swine, dairy, and poultry farms. Methods The design of the intelligent electronic nose system includes the selection of special sensors to measure odour compounds in and around the livestock farms, the selection of a chamber with a pump, the design of the electric circuits to acquire sensor signals to a computer, and software to process and analyse the measured sensor signals. Hardware design Based on previous extensive investigation on existing odour measurement technologies and equipment, as well as on various sensors available for measuring odour compounds, 16 sensors were selected for detecting the compounds of livestock and poultry farm odours and environment conditions. The 16 sensors include a temperature sensor, a humidity sensor, and 14 gas sensors that can measure almost all the major gas compounds in livestock and poultry farm odours. Some of the 16 sensors are listed in Table 1. More detailed Name of sensor Compounds to be detected Material 7NH TGS825 TGS813 TG826 .... MAX6605MXK-T HIH-3610-001 Ammonia Hydrogen-sulfide Butane Amine compounds ... Temperature Humidity MMOS Hybrid sensor Tin dioxide (SnO2 ) Tungsten oxide ... Semi-conductor Thermoset polymer Environ Monit Assess (2007) 135:399–408 401 Fig. 1 The sensor array in the chamber Fig. 3 “Odour Expert” software suite information about the sensors can be found in Tian et al. (2005). Since the sensors for odour compounds have specific requirements on the flow rate when they operate properly, a pump with “zero-link” valves is used to intake ambient air into a chamber, such that the flow rate is controlled by the pump. The chamber is like the nasal cavity of human. The sensor array is put inside the chamber. A photo of the sensor array is shown in Fig. 1. The output signals of sensors range from 0.1 to 10 mV; therefore, several electrical circuits were designed to amplify these small signals to the range accepted by the A/D (analog/digital) board. In addition, the regulation buffer was designed to transform the high output impedance of sensors into low impedance to eliminate the effects of the A/D board on the sensors, so that the sensors can accurately measure the compounds in livestock and poultry odours. The A/D converter has 32 channels to transform the analogue sensor signals into digital signals, which are then sent to the computer through a standard RS-232 serial port. Only the first 16 channels are used as there are only 16 sensors in the current design, leaving some channels open for additional sensors in future development. User Interface Help&Explain Systems Knowledge Acquisition Knowledge Base Analysis System Electrical Circuit Power Data Base Chamber Pump Laptop Fig. 2 The intelligent electronic nose with connection to a laptop computer Data Acquisition Ambient Air Fig. 4 Architecture of the proposed expert system for analysing livestock farm odour 402 The entire electronic nose with a connection to a laptop computer is shown in Fig. 2. Ambient air enter the chamber from the inlet port of the chamber; the pump is connected to the outside vacuum outlet port of the chamber, so it can evacuate air from inside the chamber at a certain flow rate (0–5 l/min); the sensor array located inside the chamber, sends the measured signals to the signal amplifier and regulation buffer; the A/D interface converts the analogue signals from the buffer to digital signals and sends them to a laptop computer; and lastly the laptop computer processes the measured sensor signals and calculated the odour intensity. Software design The signals generated by the electronic nose need to be processed and analysed. Hence, software was designed for acquiring, storing, processing, and analysing the signals. In this study, basic data acquisition software was extended into a more comprehensive expert system for livestock farm odour management. The electronic nose system is eventually used to evaluate various odour control strategies and then make recommendations on the use of those odour control techniques. These Fig. 5 Interface of the main system Environ Monit Assess (2007) 135:399–408 recommendations are useful for livestock producers to efficiently control the odour, and could offer new research directions in effective odour control technique development. An expert system called “Odour Expert” was developed for supporting odour management (Fig. 3). This expert system is not only able to conduct odour measurement from livestock farms, but also can analyse the chemical components and generation factors to determine which component or factor contributes most to the strength of odour, and then gives expert advice on what control means to take in order to efficiently reduce odour; moreover, this expert system is able to forecast the effectiveness of odour control efforts before those control means are applied. The “Odour Expert” consists of the following components: a data acquisition system for gathering data from the electronic nose, a database based on MySQL for storing data, a knowledge base for storing expert knowledge, a data analysis system for odour strength prediction and factor analysis, and a user interface to explain and interpret analysis results to users. The architecture of the proposed system is illustrated in Fig. 4. The “Odour Expert” is coded using C# programming language and MATLAB. Environ Monit Assess (2007) 135:399–408 403 Fig. 6 Data acquisition system The natural language interface provides a userfriendly environment for researchers and livestock farm operators. The interface guides the analysis procedure, interprets result to the users, and provides qualitative advice on which odour control approaches to take in order to most efficiently reduce odour level. Users can provide their specific requirements on odour control Fig. 7 Data dynamic display system operations; a list of potential control approaches is shown on the interface. Users can select the proposed potential control approach that they are interested in, then the system predicts the controlled odour level resulted from the selected control approach. Figure 5 is the main interface of the system. Pressing any of the buttons loads the corresponding subsystem. The main interface 404 Environ Monit Assess (2007) 135:399–408 Fig. 8 Data management system looks clear and concise, therefore, easy for the users to use. The data acquisition system (Fig. 6) has 16 channels to receive the digital signals that are transformed from the analog sensor signals by A/D converter. MySQL based database is used for storing odour data. The database is used by a data dynamic display system (Fig. 7) which can display Fig. 9 Data analysis system sensor signals in real time, and a data management system (Fig. 8) by which the users can organise or pre-process odour data. A knowledge base was designed to store expert knowledge. Expert knowledge used in the analysis includes type of livestock facilities, odour management methods, feeding techniques, and constraints of factors such as upper and lower Environ Monit Assess (2007) 135:399–408 405 Sensor Responses means should be applied. The controlled odour strength is forecasted if any control approach is implemented. The analysis procedure is explicit and easy to use. Sensor #1 Sensor #2 Sensor #3 Experimental implementation Sensor #4 Odour D/T Level Sensor #16 Downwind experiments were conducted on 14 Ontario livestock and poultry farms. Odour data were collected and the developed electronic nose was tested. Environmental Conditions Temperature Perceived Predicted 60 Wind Speed 50 40 30 20 10 0 0 2 4 6 Case 8 10 12 60 Data Points r=0.932 Best Linear Fit 50 40 Predicted (D/ T) bounds of wind speed, temperature, distance to the odour source and humidity. However, in current state of design, only factor constraints are considered. In future improvement, fuzzy rules will be incorporated in the expert knowledge for incorporating non-numeric odour generation factors. The data analysis system (Fig. 9) is the core of the proposed expert system. It includes odour strength prediction, factor contribution analysis, and control results prediction. The analysis system predicts odour strength based on sensor signals from the electronic nose and expert knowledge information from knowledge base, using artificial intelligent methods. A three layer neural network analysis model is employed for analysing the signals from the electronic nose (Fig. 10). The input layer has 19 input neurons, where the inputs are the signals from the 16 sensors, as well as outdoor temperature, wind speed and distance to the odour source. The output is the odour strength. A typical back-propagation neural network algorithm is applied to train the datasets. Significance of odour generation factors is ranked by using statistical factor analysis method. This rank can help users to evaluate the importance of those factors and then decide which control Output (D/ T) Distance Fig. 10 A neural network model for analysis of the signals from the electronic nose 30 20 10 0 0 10 20 30 40 50 60 Perceived (D/T) Fig. 11 Odour intensity prediction results with electronic nose signals 406 Environ Monit Assess (2007) 135:399–408 60 data collected by panelists and the electronic nose were entered into database. Outdoor temperature, wind speed and distance to the odour source were recorded as well at each site. The detailed measurement procedure is described in (Pan et al. 2007). Predicted Perceived Output (D/ T) 50 40 30 Experimental results 20 10 0 0 2 4 6 Case 8 10 12 60 Data Points r=0.809 Best Linear Fit Predicted (D/ T) 50 40 30 20 10 0 0 10 20 30 40 Perceived(D/T) 50 60 Fig. 12 Odour intensity prediction results without electronic nose signals Downwind experiments Field measurements were conducted downwind from 14 poultry, dairy and swine facilities, during a 7-week period between July and August, 2004. While at each facility, human assessors determined the odour strength in terms of odour dilution-to-threshold ratio (D/T) using a portable Nasal Ranger Field Olfactometer (St. Croix Sensory, Inc, Lake Elmo, MN, USA) (McGinley and McGinley 2004), while the developed electronic nose took measurement at the same time. All the There were totally 64 datasets available collected by the electronic nose available. Approximately 20% of the datasets were randomly selected for testing and the remaining 80% were used for training the neural network model. The neural network model was trained by a typical backpropagation algorithm. The odour strength prediction result is shown in Fig. 11. By using electronic nose, high accurate predictions were achieved (r = 0.932). The results with electronic nose signals were compared to those from human assessors. These datasets do not have the digital signals from the electronic nose, only with environmental factors, i.e. outdoor temperature, wind seed, and distance to odour source. A three layer neural network with three inputs and one output was used for the datasets, and a typical back-propagation learning algorithm was applied to train the datasets. The prediction result is shown in Fig. 12. Low accurate predictions were obtained (r = 0.809) without electronic nose signal. Comparison of predictions with electronic nose signals to those without electronic nose signals shows that sensor signals from the electronic nose aid to odour strength evaluation, helps to achieve higher accurate odour measurement. It demonstrates that the electronic nose provides accurate responds to odour strength arising from poultry and livestock farms. Factor contribution rating was implemented by using statistical factor analysis. From the results Table 2 Correlations of factors to odour intensity Factor Correlation coefficient p-value Distance Temperature Wind speed −0.3540 0.1707 0.0551 <0.01 0.01 0.4 Environ Monit Assess (2007) 135:399–408 407 35 Controlled Original 30 Output (D/T) 25 20 15 10 5 0 0 2 4 6 Case 8 10 12 Fig. 13 Control results of livestock farm odour data (Distance > 100 m) shown in Table 2, distance to the odour source is the most significant factor to the odour strength, outdoor temperature is less important, while wind speed is least significant to the odour strength. In order to investigate effectiveness of potential control strategies, for instance, beginning with the most significant factor, distances to the odour sources were considered. Odour is diluted by dispersion as it is transported in the atmosphere. The further it travels, the more it is diluted. Therefore, adequate setback distances are a key in preventing odour complaints. Distances to the odour source were set back to larger than 100 m. Figure 13 shows the control result. Odour strengths were effectively reduced. Conclusion and discussion A portable electronic nose with 14 gas sensors, a humidity sensor and a temperature sensor was successfully developed especially for objective measurement of livestock farm odours. Experiments were conducted in field downwind from 14 livestock and poultry farms. Experimental results demonstrate that “Odour Expert” is a useful tool in supporting livestock and poultry farm odour management, which greatly improves the efficiency of livestock farm odour management and thus benefit livestock industry. The electronic nose is capable of producing accurate output with greater consistency in odour measurement. It is easier and cheaper to operate than using olfactometry or human panel. Thus it has good commercial potential. However, an electronic nose can only evaluate odour events on one point; it cannot provide an overall odour mapping around livestock facilities that is necessary for an overall odour management strategy. It is suggested that an electronic nose network combined with an air dispersion model is possible to predict the downwind odour strengths on the basis of odour emission rates, topography and meteorological data. Therefore, it can locate odour strength around livestock facilities in real time, and help the development of an overall odour management strategy. References Barlett, P. N., Elliot, J. M., & Gardner, J. W. (1997). Electronic noses and their application in the food industry. Food Technology, 51(12), 44–48. Hobbs, P. J., Misselbrook, T. H., & Pain, B. F. (1995). Assessment of odours from livestock wastes by a photoionization detector, electronic nose, olfactometry and gas chromatography-mass spectrometry. Journal of Agricultural Engineering Research, 72, 291–298. Hudon, G., Guy, C., & Hermia, J. (2000). Measurement of odour intensity by an electronic nose. Journal of Air and Wastes Management Association, 50, 1750–1758. Gardner, J. W., & Barlett, P. N. (1999). Electronic noses: Principals and applications. Oxford, UK: Oxford University Press. Loutfi, A. (2006). Odour recognition using electronic noses in robotic and intelligent systems. PhD thesis, Institutionen för teknik, Örebro universitet, Örebro, Swedish. McGinley, M. A., & McGinley, C. M. Developing a credible odour monitoring program. In American Society of Agricultural Engineers (ASAE) 2004 annual conference, Ottawa, Ontario, Canada (Paper no. 042201). O’Neill, D. H., & Philips, V. R. (1992). A review of the control of odour nuisance from livestock buildings. Journal of Agricultural Engineering Research, 53(1), 23–50. Osarenren, J. O. (2002). Computer aided electronic nose for sensing odours in the dairy farms. In 2002 ASAE annual international meeting/CIGR XVth world congress, Chicago, IL, USA (Paper no. 024169). Pan, L., Yang, S. X., & DeBruyn, J. (2007). Factor analysis of downwind odours from livestock farms. Biosystems Engineering, 96(3), 387–397. Powers, W., & Bastyr, S. (2004). Downwind air quality measurements from poultry and livestock facilities. 408 Animal industry report ASL-R1924. Ames, IA: Iowa State University. Qu, G., Feddes, J. J. R., Armstrong, W. W., Coleman, R. N., & Leonard, J. J. (2001). Measuring odour concentration with an electronic nose. Transactions of the ASAE, 44(6), 1807–1812. Shaw, P. E., Rouseff, R. L., Goodner, K. L., Bazemore, R., Nordby, H. E., & Widmer, W. W. (2000). Comparison of headspace GC and electronic sensor techniques for classification of processed orange juices. Food Science and Technology, 33, 331–334. Schiffman, S. S., Bennett, J. L., & Raymer J. H. (2001). Quantification of odours and odorants from swine operations in North Carolina. Agricultural and Forest Meteorology, 108, 213–240. Schaller, E., Bosset, J. O., & Escher, F. (1998). ‘Electronic noses’ and their application to food. Food Science and Technology, 31, 205–316. Tian, F., Yang, S. X., & Dong, K. (2005). Circuit and noise analysis of odorant gas sensors in an E-nose. Sensors, 5, 85–96. Environ Monit Assess (2007) 135:399–408 Singh, S., Hines, E. L., & Gardner, J. W. (1996). Fuzzy neural computing of coffee and tainted water data from an electronic nose. Sensors and Actuators, 30(3), 190–195. Zahn, J. A., DiSpirito, A. A., Do, Y. S., Brooks, B. E., Cooper, E. E., & Hatfield, J. L. (2001). Correlation of human olfactory response to airborne concentrations of malodorous volatile organic compounds emitted from swine effluent. Journal of Environmental Quality, 30, 635–647. Zhang, Q., Feddes, J. J. R., Edeogu, I., Nyachoti, M., House, J., Small, S., et al. (2001). Odour production, evaluation and control. Final report for project MLMMI 02-hers-03. Winnipeg, Canada: Manitoba Livestock Manure Management Initiative Inc. Zhang, Q., Feddes, J. J. R., Edeogu, I., & Zhou, X. J. (2002). Correlation between odour intensity assessed by human assessors and odour concentration measured with olfactometers. Canadian Biosystems Engineering, 44, 627–632 OUR KNOWLEDGE IS YOUR SUCCESS Rapportage ontwikkeling geur sensor voor het gebruik van geurmetingen bij varkensstallen Electronic determination of odour by olfactory with the Multi Gas Analyser (MGA) Interpretation by an Artificial Neural Network Inleiding Geur meting is niet eenvoudig uitvoerbaar. Een exacte wetenschappelijk onderbouwde geurmeting moet altijd worden uitgevoerd volgens de VDI richtlijnen. Deze geurmeting is tijdrovend, duur en heeft niet de reproduceerbare nauwkeurigheid die een meetinstrument normaal heeft. De uitslagen van de geurmeting kunnen nogal veranderen. Omdat er geen goede alternatieven op de markt zijn om eenvoudig geurmetingen uit te voeren bij varkensstalen is in 2010-2011 in een project gekeken naar de haalbaarheid van een elektronische geurmeting. Tevens is een geurmeetsysteem ontwikkeld dat speciaal gebruikt kan worden bij een varkensstal. Alternatieven voor het bepalen van geur(overlast) Olfactometrie: VDI 3882 Verein Deutscher Ingenieure (VDI) (Vereniging van Duitse ingenieurs) is een organisatie van 135.000 ingenieurs en natuurwetenschappers. Opgericht in 1856 is VDI vandaag de grootste engineering vereniging in West-Europa. De rol van de VDI in Duitsland is vergelijkbaar met dat van de American Society of Civil Engineers (ASCE) in de Verenigde Staten. De VDI is geen unie. De VDI bevordert de vooruitgang van de technologie en vertegenwoordigt de belangen van ingenieurs en engineering bedrijven in Duitsland. Intensiteit waarden In (1992) wordt beschreven wat de geur intensiteit is. Omdat geur een subjectieve waarneming is wordt met behulp van de Weber-Fechner wet een relatie gelegd tussen de concentratie gas in lucht en de beleving hiervan. Geur intensiteit kan worden onderverdeeld in de volgende categorieën op basis van intensiteit: 0 1 2 3 4 5 6 geen geur zeer zwak (geur drempel) Zwak Onderscheiden Sterk zeer sterk onaanvaardbare Tabel 1 Intensiteit waarden Deze waarden worden toegekend aan een geur door een geurpanel. De geur wordt een aantal keer verdund en beoordeeld door het geurpanel. Na een serie beoordelingen kan de intensiteit en geurgrens worden bepaald. Raiffeisenstraat 24 4697 CG Sint-Annaland t +31 (0) 166 65 72 00 f +31 (0) 166 65 72 10 e [email protected] I www.macview.nl WWW.MACVIEW.NL OUR KNOWLEDGE IS YOUR SUCCESS Hedonische waarden De hedonische waarde geeft de (on)aangenaamheid van een geur aan. De hedonische waarde is een middel om de maat van geurhinder te voorspellen. +4 +3 +2 +1 0 -1 -2 -3 -4 uiterst aangenaam zeer aangenaam aangenaam enigszins aangenaam Neutraal enigszins onaangenaam Onaangenaam zeer onaangenaam uiterst onaangenaam Tabel 2: Hedonische schaal De hedonische waarde van een geur wordt doorgaans bepaald door een geurpanel en kan zowel in het laboratorium als in het veld worden bepaald. De hedonische waarde van een geur hangt af van de sterkte van een geur. Daarom wordt de hedonische waarde altijd gekoppeld aan een geuronderzoek waarbij tevens de intensiteit in geureenheden wordt bepaald. Een geur kan bij het betreden van een ruimte een hedonische waarde “onaangenaam” hebben. Door enige tijd in de ruimte aanwezig te zijn kan de geur in de beleving naar de achtergrond van het bewustzijn raken waarddor de geur enigszins onaangenaam of neutraal wordt beoordeeld. Omgekeerd zijn er reukstoffen die in eerste instantie een aangename geur afgeven. Echter na enkele minuten werken deze geurstoffen sterker in op ons reukorgaan waarbij de geur als zeer onaangenaam kan worden ervaren. Wetsvoorstel geurhinder en veehouderij Het rapport (2006) is naar aanleiding van het wetsvoorstel “geurhinder en veehouderij” geschreven. In dit rapport wordt de relatie gelegd tussen geurhinder en de intensieve veehouderij in Nederland. In tegenstelling tot de VDI richtlijnen wordt de omgeving ook meegenomen in het bepalen van de geurhinder. Dit is gedaan omdat de indeling van het landschap ook invloed heeft op het bereik van geur. Ook is aangetoond dat bewoners van een streek met intensieve landbouw veel minder last hebben van geurhinder dan bewoners in een stedelijk gebied. Om geurhinder aan te tonen wordt een sample genomen van de lucht. Deze wordt vervolgens in stappen verdund tot de geurgrens met een geurpanel is bepaald. Als deze grens is bepaald kan de geurhinder worden bepaald. In het wetsvoorstel is vastgelegd hoeveel procent geurhinder in een bepaald gebied is toegestaan. Conclusie In beide alternatieven is de concentratie gas in lucht nodig. Volgens de VDI richtlijnen kan voor verschillende gassen worden bepaald hoe groot de intensiteit en hedonische waarde is. Dit is in tegenstelling met het wetsvoorstel want hierbij wordt de emissie van veehouderij gezien als één soort lucht. In beide gevallen zijn geen parameters Raiffeisenstraat 24 4697 CG Sint-Annaland t +31 (0) 166 65 72 00 f +31 (0) 166 65 72 10 e [email protected] I www.macview.nl WWW.MACVIEW.NL OUR KNOWLEDGE IS YOUR SUCCESS beschikbaar om met de concentratie de geurhinder te bepalen. Het voordeel van het wetsvoorstel is dat er vaststaat wat de hoeveelheid geurhinder moet zijn om in overtreding te zijn. Omdat de VDI 3882 richtlijnen zijn wordt dit niet aangegeven. Aangenomen wordt dat de emissie van stallen verschillende concentraties stoffen kan bevatten. Daarom wordt gekozen voor de VDI richtlijnen omdat in tegenstelling tot het wetsvoorstel de intensiteit van verschillende stoffen kan worden bepaald. Het project "Elektronische geurmeting” Het meetonderzoek en testopstelling heeft als doel om met behulp van elektronische sensoren een betrouwbare benadering te maken van de geur intensiteit en de hedonische waarde. Het project is ingedeeld in 5 fasen. Opzet / technische plan van aanpak Om te komen tot de bepaling van de geurhinder en de intensiteit is het belangrijk dat de componenten uit een gassamenstelling bekend zijn. Als deze componenten bekend zijn kan er vervolgens een koppeling gemaakt worden naar de daadwerkelijke benadering. Schematisch gezien is de volgende opzet gebouwd: Figuur 1: Schematische opzet van de MFCs, de permeatieovens en het neurale netwerk waarin de data verwerkt wordt. Fase 1: Uitwerken lab opstelling Fase 1 is uitgewerkt. Er is een inventarisatie gemaakt van alle geurcomponenten. Deze componenten worden gesimuleerd door gebruik van permeation tubes. De permeation tubes zijn ondergebracht in 6 permeation ovens en uiteindelijk gekoppeld aan 1 systeem met Raiffeisenstraat 24 4697 CG Sint-Annaland een multi-array solid-state sensor t +31 (0) 166 65 72 00 f +31 (0) 166 65 72 10 e [email protected] I www.macview.nl WWW.MACVIEW.NL OUR KNOWLEDGE IS YOUR SUCCESS Dit systeem is een multi-array sensor geplaatst in een temperatuur geconditioneerd ontwerp waarbij met een klep nul-lucht kan worden toegevoerd en waarbij de uitkomsten van de sensor kunnen worden verwerkt in een neuraal netwerk. De stoffen die zijn geïnventariseerd zijn geëxtraheerd uit diverse wetenschappelijk publicaties. De meeste publicaties handelen over de samenstelling van gasconcentraties uit varkensmest. We zijn gekomen tot een classificatie van een top 10 aan componenten: Methyl mercaptan Phenol Pentanoic Acid/Valeric acid Butyric Acid Indole Skatole P-Cresol (4Methylphenol) Acetic Acid Ammoniak Hydrogen Sulfide Tabel 3: Overzicht van de meest aanwezige gascomponenten die aanwezig zijn in varkenslucht, verkregen uit een literatuurstudie. Naast deze componenten worden de grootheden temperatuur en RV op een traditionele wijze verwerkt door respectievelijk een PID temperatuurregeling toe te passen op de sensor en de RV te compenseren op de sensoren. Hiervoor zijn afzonderlijke testen uitgevoerd om de compensaties zo optimaal te laten verlopen. Er is dus voor gekozen om de traditionele grootheden temperatuur en relatieve vochtigheid niet in een neuraal net mee te laten analyser teneinde de resolutie van de overige componenten zo hoog als mogelijk te houden. Van alle componenten is vastgesteld wat de bovenste en de onderste geurgrenzen zijn. Dit is eveneens verkregen uit literatuurstudies. Dezelfde componenten in verschillende onderzoeken laten nogal uiteenlopende niveau’s zien. Uiteindelijk is gekozen voor de meest logische benaderingen. Kunstmatig neuraal netwerk Neurale netwerken zijn ontworpen naar analogie van het biologische brein, m.a.w. het zijn zeer uitgebreide en onderling verbonden netwerken van neuronen. Kunstmatige neurale netwerken zijn toe te passen in computersystemen. Een kunstmatig neuraal netwerk bestaat uit verscheidene (eventueel door software gesimuleerde) meestal zeer eenvoudige processoren (neuronen) met een hoge mate van onderlinge connectie waarover simpele scalaire berichten verzonden worden. De interactie tussen de diverse onderling verbonden processoren waaruit het netwerk bestaat is bovendien adaptief, zodat verbindingen tussen andere processoren in het neurale netwerk kunnen ontstaan, kunnen worden versterkt, verzwakt of weer verbroken kunnen worden. Dit betekent dat een neuraal netwerk te 'trainen' is. Het trainen van neurale netwerken gebeurt met backpropagation. Hierbij wordt een willekeurig sample ingevoerd in het netwerk en wordt vervolgens afhankelijk van de Raiffeisenstraat 24 4697 CG Sint-Annaland grootte van de fout die ontstaat de gewichten aangepast. (Jang, 1997) t +31 (0) 166 65 72 00 f +31 (0) 166 65 72 10 e [email protected] I www.macview.nl WWW.MACVIEW.NL OUR KNOWLEDGE IS YOUR SUCCESS Soorten netwerken Bekende netwerken zijn de Multilayer Perceptron netwerken en de Generalized Feed Forward netwerken. De multilayer perceptrons (MLPs) zijn netwerken die uit meerder lagen bestaan die de informatie voorwaarts doorgeven. layered feed forward netwerken worden typisch getrained met statische backpropagation (Verkregen informatie wordt terug het netwerk ingekoppeld). Dit type netwerken vind in ontelbaar veel applicatie hun weg, bijzonder daar waar een statische classificatie patroon nodig is. De voordelen van deze netwerken zijn dat ze eenvoudig zijn en dat ze vrijwel alle applicaties met alle inputsoutput structuren aankunnen. Het belangrijkste nadeel is dat de training van deze netwerken relatief langzaam gaat en ze vereisen veel trainingsdata. (Meestal meer dan 3 keer meer training samples dan netwerk gewichten.) Generalized Feed Forward Generalized feed forward netwerrken zijn een basis van de MLP netwerken, zodanig dat de verbindingen in theorie kunnen overspringen naar elk andere laag of punt in een laag. In theorie kan een MLP network alle problemen oplossen die een generalized feed forward netwerk ook kan oplossen. Echter in de praktijk is een generalized feed forward network networks vaker sneller met de oplossing en wordt het problem ook meer efficiënt opgelost. Voor het netwerk wordt gekozen voor een Generalized Feed Forward Multilayer Perceptron Network. Het voordeel van dit netwerk tegenover een standaard MLP netwerk is dat er minder trainingsdata nodig is. Het nadeel van het netwerk is dat er meer parameters zijn dus dit kost extra geheugen wat in een embedded oplossing beperkt is. De beperkte hoeveelheid trainingsdata is echter een zeer groot voordeel omdat het verzamelen van de trainingsdata relatief veel tijd kost. Topologie Na een literatuurstudie over de toepassingen van kunstmatige neurale netwerken in gasmeet technieken blijkt dat er vooral over twee soorten topologieën worden gebruikt. De eerste topologie gaat over een enkelvoudig netwerk waar de sensor verhoudingen (ratio’s) direct in het neurale netwerk worden ingevoerd. De ratio’s kunnen direct na de meting of alleen op een bepaald tijdstip worden ingevoerd. Het is ook nog mogelijk om de ratio’s eerst te conditioneren. Omdat de sensor bij alle soorten gassen een unieke stabilisatie en meettijd heeft kunnen ook alleen de unieke kenmerken van deze periode worden ingevoerd. Deze topologie is in een wetenschappelijk onderzoek gebruikt om de concentratie van één soort gas in lucht te bepalen. MGA Sensor ratio’s Conditionering van meetwaarden Geconditioneerde waarden Classificatie en concentratie netwerk Soort en concentratie gas Raiffeisenstraat 24 4697 CG Sint-Annaland t +31 (0) 166 65 72 00 f +31 (0) 166 65 72 10 e [email protected] I www.macview.nl WWW.MACVIEW.NL OUR KNOWLEDGE IS YOUR SUCCESS Figuur 2: Enkelvoudige topologie waarbij conditionering optioneel is Bij een twee lagen topologie wordt eerst welke gassen zich er in de lucht bevinden en vervolgens wordt hiervan de concentratie bepaald. Sensor ratio’s MGA Soorten gassen Classificatie netwerk Sensor ratio’s Concentratie netwerk Soorten gassen Concentratie van gassen Figuur 3: Twee lagen topologie Desired Output and Actual Network Output 70 Output 60 50 Indole 40 P-Cresol Butyric Acid 30 Indole Output 20 P-Cresol Output 10 Butyric Acid Output 0 -10 1 9 17 25 33 41 49 57 65 73 81 Exemplar Figuur 4: Relatie tussen de gemeten waarden (ononderbroken lijnen) en de gewenste output (stippellijnen) Raiffeisenstraat 24 4697 CG Sint-Annaland t +31 (0) 166 65 72 00 f +31 (0) 166 65 72 10 e [email protected] I www.macview.nl WWW.MACVIEW.NL OUR KNOWLEDGE IS YOUR SUCCESS Performance MSE NMSE MAE Min Abs Error Max Abs Error r Indole 126.2733405 0.597315442 8.371652246 0.017299954 45.54439157 0.782346579 P-Cresol 471.5152749 2.345525271 17.52764081 0.076797221 46.70068218 -0.159821897 Butyric Acid 199.1914232 1.068479668 11.04322773 0.034178076 32.69469265 0.62426319 Tabel 4: Bijbehorende afwijkingen van de gemeten waarden en de gewenste waarden. Desired Output and Actual Network Output 70 Output 60 50 Indole 40 P-Cresol Butyric Acid 30 Indole Output 20 P-Cresol Output 10 Butyric Acid Output 0 -10 1 3 5 7 9 11 13 15 17 19 21 23 25 27 29 31 33 35 Exemplar Figuur 5: Relatie tussen de gemeten waarden (ononderbroken lijnen) en de gewenste output (stippellijnen) Performance MSE NMSE MAE Min Abs Error Max Abs Error r Indole 29.94917093 0.110922855 3.988914929 0.247990571 20.28415467 0.951154943 P-Cresol 199.2941651 0.738126537 11.10540487 0.489905225 29.0716076 0.73565366 Butyric Acid 53.11927596 0.196738059 5.480077289 0.000933466 17.21323142 0.920110634 Tabel 5: Bijbehorende afwijkingen van de gemeten waarden en de gewenste waarden. Grafische voorstelling en tabel van enkele resultaten uit het project van een training in een neuraal netwerk. Raiffeisenstraat 24 4697 CG Sint-Annaland t +31 (0) 166 65 72 00 f +31 (0) 166 65 72 10 e [email protected] I www.macview.nl WWW.MACVIEW.NL OUR KNOWLEDGE IS YOUR SUCCESS Permeatie-ovens Een zeer speciaal aandachtspunt bij de opbouw van de opstelling waren de permeatie-ovens. De belangrijkste vraag bij de opbouw was: Hoe maken we geurcomponenten waarmee we op ppb niveau reproduceerbaar, relatief goedkoop en heel nauwkeurig kunnen kalibreren. EMS heeft naar aanleiding van deze vraag zelf 6 verschillende ovens gebouwd. De nauwkeurigheid is factoren hoger dan de versies die commercieel verkrijgbaar zijn. Met deze 6 ovens zijn allerleis soorten combinaties van stoffen samengesteld waarmee kon worden gemeten. De software van de MFCs in combinatie met de permeatieovens is in staat om de concentratie van een geurcomponent tot op een concentraties van 1 tot 2 ppb te regelen binnen een nauwkeurigheid van +-2.5%. Figuur 6: Achterzijde van de permeatie-oven met flowcontrollers en gaswasflessen. MGA De MGA is een elektronisch systeem dat is opgebouwd en ontworpen op basis van een solid-state Array sensor. Deze sensor heeft meerdere sensoren (receptoren) waarbij elke sensor verschillende eigenschappen meegegeven kan worden. Dit kan gedaan worden door de aansturing van de sensoren m.b.v. elektrische parameters te beïnvloeden. (Temperatuur, stroom, spanning, frequentie etc.) Een kenmerk is dat deze sensordata door een mens moeilijk te interpreteren is. Echter voor een neuraal netwerk is deze informatie goed te interpreteren. De MGA is geassembleerd opgebouwd en getest. Er is een temperatuurregeling aangebracht om e.a. te stabiliseren en geen condensatie te laten plaatsvinden. De output van de MGA bestaat uit een string met data die elke seconde wordt afgedrukt naar een PC. Deze data wordt door het neurale netwerk gebruikt om de Raiffeisenstraat 24 4697 CG Sint-Annaland conclusies te trekken. t +31 (0) 166 65 72 00 f +31 (0) 166 65 72 10 e [email protected] I www.macview.nl WWW.MACVIEW.NL OUR KNOWLEDGE IS YOUR SUCCESS Figuur 7: Opgebouwde MGA voordat deze is ingebouwd in een (portable) kast voor in het veld. Fase 2: Inleren lab opstelling Het principe van werken met neurale netwerken is een uiterst geschikte oplossing voor geursensoren. Het grote nadeel is dat er heel veel trainingsdata nodig moet zijn om alle mogelijke combinaties te trainen en te testen. Een oplossing hiervoor hebben we gevonden in een afweging te maken door te kiezen voor een lager resultaat in de uitkomsten, maar een hogere snelheid met inleren. Het inleren is gebeurd door in stappen van verschillende concentraties stoffen aan de Multi Gas Analyser aan te bieden. In eerste instantie is dit uitgevoerd in kleine stappen. Er zijn 2 stoffen aangeboden om vervolgens te onderzoeken of het inleren van stoffen ook daadwerkelijk een significant resultaat opleverde. Na enige verbeterslagen was dit optimaal en is begonnen om elke gascomponent in verschillende stappen in te leren. Hierbij spelen reproductie van data, nauwkeurigheid van het systeem en stabiele omgevingsfactoren een cruciale rol. Raiffeisenstraat 24 4697 CG Sint-Annaland t +31 (0) 166 65 72 00 f +31 (0) 166 65 72 10 e [email protected] I www.macview.nl WWW.MACVIEW.NL OUR KNOWLEDGE IS YOUR SUCCESS Fase 3 / 4: Onderzoek inleren geurwaarde en \hedonische waarde lab / praktijk Een belangrijke stap is de vertaalmethode om informatie uit een neuraal netwerk omgezet te krijgen naar een intensiteit of een hedonische waarde. We vermelden er bij dat we het resultaat van de uitkomst uit de elektronische neus zo dicht als mogelijk de intensiteit en de hedonische waarde willen laten benaderen aan de methoden zoals beschreven in de VDI richtlijnen. De methodiek om deze waarden te koppelen is als volgt opgezet: (Zeer kort samengevat daar de onderliggende complexiteit te ver voert voor dit verslag) De intensiteit is een logaritmische waarde. De sensoruitslag is eveneens een logaritme. Dit betekend dat deze waarden min of meer lineair met elkaar te vergelijken zijn. Een intensiteit met een waarde van 6 heeft een bijbehorende gasconcentratie in ppb / ppm. Deze waarde gebruikt men in het inleren van het neurale netwerk en stelt men gelijk aan waarde 6. De verhouding die hieruit voortkomt is een quasi lineaire benadering die ook in een aantal onderzoeken als zodanig wordt bevestigd. (Kwantitatieve benadering). De hedonische waarde is een vast getal per stof. In ons model berekenen we voor alle stoffen een partiële hedonische waarde naar de verhouding van het aantal stoffen dat kwalitatief wordt bepaald. Het totaal van deze partiële stoffen vormt de grondslag voor de optelsom van alle partiële hedonische waarden en vormt zodoende de totale hedonische waarde van een mengsel aan gassen. (Deels kwantitatief en kwalitatieve benadering). Fase 5: Uitwerken resultaten, aanbrengen nieuw gevonden resultaten Het resultaat van dit project is dat we een werkend systeem hebben gebouwd dat in het veld ook is gebruikt en dat bestaat uit een kast waarin elektronica is verwerkt met daarin een zelfaanzuigende pomp, een paar kleppen en een display. Deze kast werkt op 220VAC en stuurt elke seconde een datastring uit. Daarnaast staat een PC die de datastring verwerkt. De data wordt verwerkt in een neuraal netwerk. Op de PC is software geschreven waarmee de data kan worden geïnterpreteerd en visueel worden weergegeven. Uiteindelijk wordt in een gassamenstelling bepaald welke van de eerder genoemde10 majeur aanwezige geurcomponenten aanwezig zijn. (Kwalitatieve bepaling). Daarna wordt voor elke component ook bepaald wat de gasconcentratie is berekend naar genormaliseerde grootheden in ppbs. (Kwantitatieve bepaling). Vanuit deze informatie wordt met behulp van een kleine database de intensiteit en de hedonische waarde van een gas bepaald. De PC heeft een database waarbij de gevonden meetwaarden ook opgeslagen worden. De meetinterval voor een meting is circa 15-45 minuten. Dit komt omdat de sensoren opnieuw moeten worden gereinigd en hangt af van de concentratie van een gassamenstelling. Gezegd moet worden dat er op dit moment geen systemen zijn voor het meten van geur uit varkensmest die zo snel de geur kunnen bepalen. Er is nog altijd een combinatie van een PC en een neuraal netwerk nodig. Echter de werking verloopt zeer goed. De overeenkomsten met menselijke interpretatie van de beleving intensiteit en hedonische waarde komt goed overeen. Conclusie / aanbevelingen Er is een systeem gebouwd waarmee geur op zowel kwantitatieve alsook kwalitatieve alsook op kwantitatieve hoeveelheden beoordeelt. De intensiteit en de Raiffeisenstraat 24 4697 CG Sint-Annaland t +31 (0) 166 65 72 00 f +31 (0) 166 65 72 10 e [email protected] I www.macview.nl WWW.MACVIEW.NL OUR KNOWLEDGE IS YOUR SUCCESS hedonische waarden worden bepaald. De relatie naar een menselijk geurpanel is niet gemaakt. Wel is de verwachte output gecontroleerd met echte gasconcentraties in die in een labomgeving zijn aangeboden. Daarin komt de uitslag overeen en is het systeem reproduceerbaar. Aanbevelingen voor verdere verbetering zijn miniaturisatie, de opname van een neuraal netwerk in een kleinere processor, en een mogelijke methode om de inleer-procedure / kalibratie procedure te versnellen of generiek te maken voor alle sensoren uit eenzelfde serie. Verder mag de meting versneld worden om meer real-time data te kunnen verkrijgen. De praktische uitvoering en het gebruik van deze sensor is onder handbereik, maar hangt af van de vraag van de overheden en de handhavende instanties. Figuur 8: Voorzijde met de 6 permeatie-ovens en bovenop de ingebouwde MGA sensor. Raiffeisenstraat 24 4697 CG Sint-Annaland t +31 (0) 166 65 72 00 f +31 (0) 166 65 72 10 e [email protected] I www.macview.nl WWW.MACVIEW.NL OUR KNOWLEDGE IS YOUR SUCCESS Figuur 9: Programma voorbeeld van de MGA2Neural programma waarbij de intensiteit en de hedonische waarde worden weergegeven op het scherm van de PC. Raiffeisenstraat 24 4697 CG Sint-Annaland t +31 (0) 166 65 72 00 f +31 (0) 166 65 72 10 e [email protected] I www.macview.nl WWW.MACVIEW.NL