Location

Overview
Magellan Bioscience
Business and Capabilities
Applications of Marine
Microorganisms
Overview of Collection and
Process Development
Magellan BioScience
Group, Inc.
• Location: Tampa, Florida
• Founded: 1997
• What do we do?
o Natural products discovery company
o Use novel technology to screen microbial products for industrial
applications
o Provide extracts and services based on clients’ needs
Business Models
NRDO- No Research Development Only…..
RIPCO/RIBCO- Royalty Income Biotechnology Company
FIPCO/FIBCO- Fully Integrated Biotechnology Company
Technology Platform (Research Only)- Rent/Sells technology
Magellan - Hybrid
Clients
Pharmaceutical
Agrochemical
MAGELLAN
BIOSCIENCE
Cosmetic
Animal
Health
Care
MAGELLAN ANALYTIX
Specialty
Chemical
Food and
Flavor
Energy
Internal
Research
Internal Research Efforts
•Novel plant growth promoter – induces growth more than 20% over
controls and induces salt tolerance
•Anti-inflammatory/Anticancer product oil
•Nonsteroidal animal growth promoter
•Nutraceuticals:
Urinary tract Health
Irritable bowel Syndrome
•Pharmaceuticals:
Abuse resistant technology
Magellan- Recent Developments
MAGELLAN ANALYTIX
Nutraceuticals
21 CFR 111
Pharma –Like
Research (GLP)
Pharmaceuticals
21 CFR 211
Capabilities
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Fermentation
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Fractionation, Separations
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Isolation, Structure Elucidation
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Synthetic Modifications
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Microbial Biotransformation
Distribution of Marine Natural
Products by Phylum
Molluscs
7%
Coelenterates
21%
Tunicates
7%
Echinoderms
3%
Microorganisms &
Phytoplankton
15%
Green algae
1%
Sponges
38%
Red algae
6%
Personal Communication from John Blount
Brown algae
2%
As the number of compounds from terrestrial microbes decline…
Novel compounds from marine microbes increase...
Discovery Process for Novel Leads from
Marine Natural Products
Microbial Dereplication
(Select Subset of Cave Microorganisms)
Why Use Marine Microorganisms ??
Untapped source of new products
Robust and ‘simple’ to culture
Seasonably independent
Environmentally benign
Products are natural
Sustainable, renewable, consistent, long-term supply of raw
materials for fermentation
• Rapid scale-up and production
• Independent of Oil and Natural Gas Supplies
• DNA preserved for future manipulation
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Microbial Metabolites
• Primary metabolites – essential chemical compounds that
play direct role in growth, development or reproduction
• Secondary metabolites – nonessential for life sustaining
activity
• Why are they important?
They can be applied to various products as efficient active
components due to their properties and functions
Vitamins
Antibiotics
Uses
Colorants
Detergents
Classes of Products Produced by
Marine Microorganisms
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Biosurfactants
Pigments
Enzymes
Polysaccharides
Proteins
Antioxidants
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Peptides
Fatty acids
Bioadhesives
UVA absorbers
Polyhydroxyalkanoates
Terrestrial vs. Aquatic
Terrestrial Biosphere
• Accessibility - Easy to obtain
• Number of new products is dwindling
Marine Biosphere
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High potential
Higher Risk
Microorganisms account for 98% biomass
Unique biodiversity - extreme environments
Bioprospecting
Diverse locations such as the Caribbean, Hawaii, New
England, Bermuda, Arctic and Antarctic etc.
• Deep ocean sediment
• Shallow coral reefs
• Water sampling
• Marine caves
Adhere to guidelines of the 1992 Rio Treaty on Biodiversity
• Obtain informed consent from foreign countries prior to collecting
• Equitable agreement to share a percentage of profits
• Avoid being labeled “biopirate”
Summary of Magellan’s Microbial Extract Process
Collection and Culturing
Divers given instructions
• Collect microorganisms associated
with macroorganisms (sponges,
tunicates, echinoderms, etc.)
Samples serial diluted in
proprietary medium
• Must be capable of surviving at
room temperature
Identification and
Cryopreservation
• Microbial Strain Purification
• Identification
o Look for unique colony forming units
• Phenotypic characteristics
• Pigmentation
• Zone of inhibition, etc…
o Fatty Acid Fingerprinting – check for duplicate samples
• Cost effective and just as good as DNA sequencing
• Cryopreservation
Isolates
Libraries
• Over 10,000 cultureable marine microbes
• Over 500 endophytic fungal samples
Extracts
• Over 500 extracts (macroorganism)
• Obtained from several geographic locations
• Biologically active compounds
Cultivation Process
• Active projects kept on shakers at room temperature. Multiple
copies kept in different freezers
• All work is conducted in laminar-flow hoods
Freeze-drying
• Lyophilization
• Purpose – dehydrate trays by removing water
• Result – concentrated microbe & its products
Extraction– Filtration
Process
• Proprietary organic solvent extraction of lyophilized material
• Separates microorganisms from metabolites
Extract
Plating
• Mother plates –
standard 96 wells
• Store for
cryopreservation
• Ship to client