Faculty of Electrical Engineering
Transcription
Faculty of Electrical Engineering
UNIVERSITI TEKNOLOGI MALAYSIA 1 DECLARATION OF THESIS / UNDERGRADUATE PROJECT PAPER AND COPYRIGHT MAL UNIVERSITI TEKNOLOGI MALAYSIA Author’s full name : AYSIA Date of birth : SITI NORFATIHA BINTI ISMAIL 25 APRIL 1990 Title : AUTOMATED OZONE INJECTION SYSTEM FOR MUSHROOM INDUSTRY Academic Session : 2012/2013 I declare that this thesis is classified as: CONFIDENTIAL (Contains confidential information under the Official Secret Act 1972)* RESTRICTED (Contains restricted information as specified by the organization where research was done)* OPEN ACCESS I agree that my thesis to be published as online open access (full text) I acknowledged that Universiti Teknologi Malaysia reserves the right as follows: 1. The thesis is the property of Universiti Teknologi Malaysia. 2. The Library of Universiti Teknologi Malaysia has the right to make copies for the purpose of research only. 3. The Library has the right to make copies of the thesis for academic exchange. Certified by: SIGNITURE 900425-10-5152 (NEW IC NO. /PASSPORT NO.) Date: NOTES : SIGNATURE OF SUPERVISOR ASSOC. PROF. DR. ZOLKAFLE BIN BUNTAT NAME OF SUPERVISOR Date: * If the thesis is CONFIDENTAL or RESTRICTED, please attach with the letter from the organization with period and reasons for confidentiality or restriction. 2 “I hereby declare that I have read this thesis and in my opinion this thesis is sufficient in terms of scope and quality for the award of Bachelor of Electrical Engineering” Signature :……………….……………… Supervisor : ASSOC. PROF. DR. ZOLKAFLE B. BUNTAT Date :…….…….………………..…. i AUTOMATED OZONE INJECTION SYSTEM FOR MUSHROOM INDUSTRY SITI NORFATIHA BINTI ISMAIL A thesis submitted in partial fulfillment of the requirements for the award of the degree of Bachelor of Electrical Engineering Faculty of Electrical Engineering Universiti Teknologi Malaysia JUNE 2013 ii I declare that this thesis entitle “Automated Ozone Injection System for Mushroom Industry” is the result of my own studies except as cited in the references. Signature :………………………………………… Name : SITI NORFATIHA BINTI ISMAIL Date :………………………………………… iii This thesis is dedicated to my beloved parents for their endlessly loves, supports and encouragements… iv ACKNOWLEDGEMENT Alhamdulillah, praised to ALLAH, I managed to successfully complete my final year project. First of all, I would like to express my deepest gratitude to my supervisor, PM. Dr. Zolkafle B. Buntat for willing to accept and supervised me throughout the year. Other than that, I also would like to thank my family for their supports and advices that always keep me motivated. My greatest appreciation also goes to Muhammad Fariz Izzwan B. Zaki for his guidance and ideas in order for me to accomplish this project. Other than that, I would like to thank all my friends especially Nur Afifah Abd Halim and Siti Soleha Ab Ghani for always being with me through my upsides down. Lastly, big thanks to Universiti Teknologi Malaysia especially to Faculty of Electrical Engineering for giving me a chance to completing my undergraduates study here. v ABSTRACT Recently, the awareness about the benefits offered by mushrooms has increased among citizens in our country. All types of mushrooms especially oyster mushroom supplies a lot of nutrient and vitamins needed by our body. It is not only prepared as food but it also has medicinal values. The demands for mushrooms are very high in international and local market. Hence, the mushroom industry was classified as a good potential industry to be commercialized since there were very high demands of it. The present treatment methods of mushroom substrate were not really productive since it is time consuming and less in production. Therefore, in this project, an introduction of automatic ozone injection system to sterilize mushroom substrates has been proposed. In this project, there are three approaches used. First, literature reviews were done to get a clear view of the mushroom industry. Second, a prototype design and an animation to illustrate the operation of the machine were developed by using Solidworks software. Lastly, by using OMRON CX-One Programmer, a controller was constructed to automate the machine operation. The prototype designs consist of three main parts which are the conveyor, the injection panel and also the substrates mold. The controller was programmed so that the injection process must be within 30 minutes only since the purpose of inventing the machine was to reduce time consuming. At the end of the study, it can be concluded that an automated mushroom substrates treatment machine that can improve the cultivation of mushroom was successfully established. However, it is recommended that in the future the hardware of this prototype can be developed to prove the validity of this system. vi ABSTRAK Kesedaran tentang faedah yang ditawarkan oleh cendawan telah meningkat dalam kalangan rakyat di negara kita di kebelakangan ini. Cendawan terutamanya dari jenis tiram, membekalkan banyak nutrien dan vitamin yang sangat diperlukan oleh badan kita. Ia bukan sahaja boleh dijadikan sebagai makanan tetapi ianya juga boleh dijadikan bahan bagi tujuan perubatan. Permintaan terhadap cendawan adalah sangat tinggi sama ada dalam pasaran tempatan mahupun antarabangsa. Oleh kerana permintaan terhadap cendawan adalah tinggi maka industri ini boleh diklasifikasikan sebagai industri yang berpotensi untuk dikomersialkan. Kaedah rawatan semasa untuk substrat cendawan tidak benar-benar produktif kerana proses tersebut memakan masa yang lama dan hasil pengeluarannya juga kurang. Oleh itu, dalam projek, ini pengenalan terhadap sistem suntikan ozon secara automatik untuk mensterilkan substrat cendawan telah dicadangkan. Di dalam projek ini, terdapat tiga pendekatan yang telah digunakan. Pertama, ulasan kesusasteraan telah dilakukan untuk mendapatkan gambaran yang jelas mengenai industri cendawan dan cendawan itu sendiri. Kedua, reka bentuk prototaip dan animasi untuk menggambarkan operasi mesin telah dibuat dengan menggunakan perisian Solidworks. Akhir sekali, dengan menggunakan perisian OMRON CX-One Programmer, sebuah alat kawalan telah dibina untuk mengautomasikan operasi mesin. Reka bentuk prototaip terdiri daripada tiga bahagian utama iaitu penghantar, panel suntikan dan juga acuan substrat untuk diisi bongkah cendawan semasa proses suntikan. Alat kawalan ini telah diprogramkan supaya proses suntikan mestilah berlaku hanya dalam masa 30 minit sahaja kerana tujuan mesin ini dicipta adalah untuk mengurangkan pengambilan masa bagi proses rawatan substrat. Kesimpulannya, di akhir kajian ini, sebuah mesin suntikan ozon secara automatik untuk rawatan substrat yang boleh meningkatkan penanaman cendawan telah berjaya dibangunkan. Walau bagaimanapun, adalah disyorkan bahawa pada masa hadapan perkakasan prototaip ini dapat dibangunkan bagi membuktikan keberkesanan sistem ini. vii TABLE OF CONTENT CHAPTER TITLE 1 2 PAGE DECLARATION ii DEDICATION iii ACKNOWLEDGEMENT iv ABSTRACT v ABSTRAK vi TABLE OF CONTENT vii LIST OF TABLE ix LIST OF FIGURE x LIST OF ABBREVIATION xi LIST OF SYMBOL xii INTRODUCTION 1 1.1 Background of the Study 1 1.2 Problem Statement 2 1.3 Objectives 2 1.4 Scopes 3 LITERATURE REVIEW 4 2.1 Introduction 4 2.2 Mushrooms 4 2.3 Edible Mushroom 5 2.4 Pleurotus Ostreatus (Oyster Mushroom) 5 2.5 Mushroom Cultivation 5 2.6 Mushroom Substrate Treatment 7 viii 3 4 Autoclaves Treatment 7 2.6.2 Hot Water Treatment 8 2.6.3 Chemical Sterilization 8 2.6.4 Oil Drum Pasteurization 9 2.6.5 Ozone Treatment 9 RESEARCH METHODOLOGY 10 3.1. Introduction 10 3.2. Project Flow 10 3.3. Solidworks 2012 11 3.4. OMRON CX-One Programmer 13 3.4.1. PLC Program Flow Chart 13 RESULTS AND DISCUSSIONS 16 4.1 Introduction 16 4.2 Prototype Design 16 4.2.1 Conveyor 17 4.2.2 Injection Panel 18 4.2.3 Substrates Mold 19 4.2.4 Ozone Generator 20 4.2.5 Ozone Tank 21 4.2.6 Monitoring Unit 22 4.2.7 The Machine 23 PLC Controller 24 4.3.1 The Sequence of the Controller Process 25 4.3.2 CX-One Programmer Simulation 26 4.3 4.4 5 2.6.1 Animation 26 CONCLUSION AND RECOMMENDATION 28 5.1. Conclusion 28 5.2. Recommendation 28 ix REFERENCES 29 APPENDICES A -B 30 x LIST OF TABLES TABLE TITLE PAGE 2.1 Import statistics of mushroom in 2004 6 2.2 Export statistics of mushroom in 2004 6 xi LIST OF FIGURES FIGURE TITLE PAGE 2.1 Auto-claves process 8 3.1 Flow of the project 11 3.2 User interface 12 3.3 PLC programming flow chart 14 4.1 The conveyor in multiple views 17 4.2 Injection panel 18 4.3 Series of needles in the panel 18 4.4 Injection panel’s holder in multiple views 19 4.5 The substrates mold in multiple views 20 4.6 Ozone generator 21 4.7 Ozone tank 22 4.8 Monitoring unit 23 4.9 Isometric view of the automated treatment machine 24 4.10 Ladder diagram 25 xii LIST OF ABBREVIATIONS UI - User Interface PLC - Programmable Logic Control HMI - Human Machine Interface 1 CHAPTER 1 INTRODUCTION 1.1 Background of Study Recently, the awareness about the benefits of mushrooms has increased among citizens in our country. Mushrooms are not only used when preparing food but it also can be used for medical purposes and as a supplement [1]. There are various types of mushrooms available in the world. However, only edible mushrooms can be consumed by human- being. Oyster, Portobello, Shiitake, Black Truffles and Button are common types of edible mushrooms. Each type of mushroom has its own benefit. For instance, oyster mushroom was said to be a cure for HIV disease because it contains high compound of anti-oxidant [2]. However, this matter is still in research. Other than that, it also can help people to reduce their cholesterol level. Mushrooms are not only good in taste, but it also contains high nutrient and also have medicinal values. Since more people started to become aware about its benefits, mushroom demands increased day by day. However, the production of mushroom in our country is still low and cannot fulfill consumer’s demand. Besides, present methods of mushroom substrate treatments are also not really productive and this factor also leads to limited production of mushrooms. As we know, good substrates determine the profitability of the industry. Hence, if there is lack of good substrates produced, the industry will suffer a profit loss. 2 1.2 Problem Statement There is a very high demand for mushrooms especially for oyster types among consumers. This is because the people nowadays started to realize the benefits of mushrooms. However, the production of mushrooms is still limited because of the substrate disease problem such as substrate was affected by fungi that can harm the mushroom cultivation. A good substrate was said to be the important key in order to determine the profitability in mushroom cultivation industry [4]. The mushroom industry is a very potential market to be involved in because it is cheap to produce but profitable [1]. However, the present treatments of mushroom’s substrate such as autoclaves method are not really productive because it is time consuming. Hence, it is the right time to propose the newest method of mushroom treatment which is the ozone treatment. Since ozone treatment is a new treatment method, it is not too exposed to the industry. Hence, to commercialize this method, an automated ozone injection machine should be introduced so that it can enhance mushroom cultivation by producing a large scale of good substrates. 1.3 Objectives The objectives of this project are: 1. To design the prototype of the new ozone treatment by using Solidworks software. 2. To develop animation in order to express the operating of ozone treatment system for mushroom substrate sterilization 3. To construct the controller of the machine in order to automated its operation by using PLC controller 4. To come out with an automated treatment machine to improve the cultivation of mushroom especially oyster mushroom. 3 1.4 Scopes There are three main scopes that were focused in this project. The scopes are: 1. Designing an automated machine which integrates a conveyor that can be used for ozone injection into mushroom substrates to enhance mushroom cultivation. 2. Developing a controller system that can automates the treatment machine operation. 3. Developing the system animation to illustrates the operation of the machine 4 CHAPTER 2 LITERATURE REVIEW 2.1 Introduction In this chapter, overviews and theories about mushrooms, mushroom cultivation and also the present method of mushroom substrate sterilization were discussed. Overviews on these topics are essential in order to give clear views about mushroom itself and why this project is conducted. 2.2 Mushrooms Mushroom is defined as a group of fungi which is fleshy and has a domed shape on top of its stalk [5]. It is a fast growing population and it also can be grown either in wood, soil or in decaying metal such as saw dust [1]. There are many types of mushroom available in this world but only a few of them are non-toxic and safe for human being which is called edible mushroom. Mushroom’s contents were said to consist 90% of water while the other remaining 10% is from drying matter [6]. It was estimated that there were about 12,000 species of fungi that can be classified as mushrooms. However, only 2,000 out of the amount were edible and safe for human. 5 2.3 Edible Mushroom Edible mushroom is a class of mushroom that is safe for human. It is nontoxic and can be used for cooking, for medical purposes and even as a supplement for having a healthy body. Edible mushrooms can either be harvested wild or cultivated [7]. There are many types of edible mushroom available, for instance; Oyster, Portobello, Shiitake, Black Truffles and Button that offers hundreds of benefits to human. 2.4 Pleurotus Ostreatus (Oyster Mushroom) Pleurotus Ostreatus or commonly known as Oyster mushroom is a famous type of mushroom even in our country. This type of edible mushroom is very popular among humans because it is tasty and also nutritious since it contains most of the nutrients needed by human body such as vitamin, protein and also mineral [8]. Hence, oyster mushroom is a suitable food which can serve as a nutritious daily diet for all types of age. Other than that, oyster mushroom can help people who suffer from anemia to recover since it can improve hematopoietic factors in human body. Besides that, it also can help in reducing cholesterol level since it contains low caloric value and also less in fat. As a result, oyster mushrooms are chosen the most by consumers because it brings a lot of benefits for their health. 2.5 Mushroom Cultivation Mushrooms especially edible mushrooms can be wildly harvested or cultivated. Mushroom cultivation industry was said to be a profitable industry [4]. 6 This is because there are high demands of mushroom from customers from both local and international market. Internationally, China is the largest producer of mushrooms which contributes almost 85% of the total world production while in Malaysia, Selangor was identified as the largest producer of mushrooms. Table 2.1 and Table 2.2 below show the statistics of import and export of mushrooms in 2004. However, the value was expected to increase from year to year. Table 2.1 Import statistics of mushroom in 2004 [17] Table 2.2 Export statistics of mushroom in 2004 [17] In addition, the cost to produce mushroom is also very low since man power needed to cultivate mushroom is cheaper compared to others [4, 1]. Other than that, nowadays there are a lot of initiatives taken by the government to enhance mushroom cultivation in our country such as courses for mushroom cultivation for new entrepreneur and also researches about mushroom cultivation that has been jointventured by some universities 7 2.6 Mushroom Substrate Treatment Mushroom substrate treatments are essential in order to ensure that the mushroom product is in high quality. Sterilization is one of them. Sterilization is defined as any process of eliminating transmissible agents [13]. Hence, treatment was done so that it can kill bacteria such as fungus that will contaminate and affect the substrate during the cultivation process. 2.6.1 Autoclaves Treatment Autoclaves treatment is one of the methods of sterilization for mushroom substrate preparation. It kills bacteria by giving them a high pressure of saturated steam at the temperature of 121˚C [9]. During the treatment process, substrates will be placed in polypropylene bags and autoclaved in a device that is similar to a rice cooker for several hours in order to ensure that all the bacteria that contaminated the substrates will be killed. However, there was a study reported that this type of sterilization method was a cost consuming method even though it can produce high yields [6]. It is because it used high electricity during the process. Other than that, this method was also time consuming since the time was calculated starting from the heating process until the cooling down process [14]. It took six to ten hours to complete the sanitizing process while for cooling down process it took nearly 10 hours. Figure 2.1 shows the process of autoclaving. 8 Figure 2.1 2.6.2 Auto-claves process [15] Hot Water Treatment Hot water treatment is a treatment using hot boiling water in order to remove bacteria in substrates. During the treatment, substrates will be soaked into hot boiling water to produce sanitation against bacterial disease that can affect the growth or the cultivation process of mushrooms. Even though this method is cheaper in cost than the auto-claves method but it is really time-consuming since it also has a cooling down process. Furthermore, the productions of mushroom are also less with this method since this method is more suitable for small scale industry. 2.6.3 Chemical Sterilization Chemical sterilization was done by soaking the substrates into a chemical solution for instance ethylene oxide to reduce the contaminating agents. However, this method of sterilization was not really recommended because it is feared that the chemical agents will reduce the nutrient contains in the substrate. 9 2.6.4 Oil Drum Pasteurization Oil drum pasteurization was done by using heated water and grate. The grate was placed into a notch in the drum. Then, water was filled into a drum approximately 15cm below the grate. After that, the prepared substrates bags were placed into the grate in stack layer. Then, when all the prepared bags have been placed, the lid rim will be sealed. In the meantime, the water was started to boil until four to six hours when the vapor starts to rise. The pasteurization time depends on the substrates materials and also the size of the bag [16]. However, this method is not suitable for commercializing since the production of substrate is less. 2.6.5 Ozone Treatment Ozone is defined as a powerful reactive of triplet oxygen molecules which is bonded together [11]. It is very oxidative and powerful in sterilizing. Hence, it is widely used in food producing industry since it is eco-friendly. Ozone treatment is best used in order to avoid anti-fungal disease that can either be applied for plants such as mushrooms or also to water as treatment agents. In mushroom cultivation industry, by using ozone, bacteria that affect the mushroom cultivation can be killed in a very short period of time depending on the concentration of the ozone itself [3]. The concentrated the ozone, the less time needed to kill the bacteria. Ozone treatment is a new technology in mushroom industries for sterilization of mushroom substrate. However, this method was not yet to be commercialized. This treatment method is very environmental friendly. Besides, the production of mushroom will be higher compared to other methods of treatments. Furthermore, due to the oxidation process with the organic matter, the nutrient in the mushroom substrates also will be raised by using this new method [14]. The best thing is, by using this method; the sterilization process will be efficient in time and also in cost. 10 CHAPTER 3 RESEARCH METHODOLOGY 3.1 Introduction There are three methods and approaches that were used in order to complete this project. First, literature reviews from books and journals about mushroom cultivation and present mushroom treatment were done in order to get a clear view about the mushroom industry itself. Secondly, by using Solidworks software both designing of the automated treatment machine and also the animation to illustrate the operation of the machine was developed. Lastly, a PLC programmer which is OMRON CX-One was used to construct a controller that automates the machine operation. 3.2 Project Flow A flow chart in Figure 3.1 illustrates the flow of the project. 11 Start Literature reviews Designing process Animation developing Controller designing Complete project End Figure 3.1 3.3 Flow of the project Solidworks 2012 Solidworks 2012 is the latest version of Solidworks software. It is a powerful software that can be used for designing a prototype of any computer aided design (CAD). Other than that, this software also can be used in order to develop animation. Hence, Solidworks is the best software to be chosen for completing this project because it contains both designing and animation developer. 12 In addition, this software is also more user friendly compared to other similar softwares. There are some advantages of using this software which are; 1. Solidworks has a function of auto-checks and also autocorrects that can automates the reviewing and correction process. 2. Solidworks has its own online forum so that designers and engineers all over the world can compare their works and also ask for advice regarding their designs from the community in the forum. 3. Solidworks has a Microsoft structural storage format and this makes it more user-friendly as we know that everyone knows how to use Windows. 4. Solidworks is a compatible software because it can work with various data format. 5. Solidworks are easily accessible software at any time and flexible. Figure 3.2 below illustrates the function of toolbars that are available in Solidworks software and also its user interface (UI). Figure 3.2 User interface (UI) 13 3.4 OMRON CX-One Programmer CX-One programmer by OMRON is one of the best software for programming activities. It enables the user to build, configure and build networks especially for PLCs, HMIs, motion control system and sensors. The result of this software was reduced in complexity. Hence, an automation program can be easily programmed. Other than that, the graphical interface for this software is also user friendly. The simple integration reduces training need and user can learn to use the software by self-learning. The general appearance of this software which is the menus and the function is simple and quite similar with other softwares. In addition, this software also has a good documentation so that it eases the user to search and open the file. Furthermore, this software has easy project management. The file management operates in the background of automatically saving in one common directory. As a result, a backup of a project is easy to make. 3.4.1 PLC Program Flow Chart Before simulating the PLC program in CX-One programmer, a flow chart must first be made. The flow chart is essential in order to have a clear picture about the flow of the automation machine. Then, the flow chart will be transformed into the ladder diagram in CX-One programmer that will be discussed in the next chapter. Figure 3.3 describes the flow chart for the PLC programming. 14 Start Loaded molded tray with mushroom substrates No Check whether the mushroom substrates is fully loaded in the tray Yes Injection panels are ready for injection process -green lights turn on Injection panel holder going down and covered the tray -process within 30 seconds Ozone injection process is started -process within 30 minutes -red light turn on 15 Injection panel holder going up -process within 30 seconds Sufficient ozone injected No Yes Conveyor conveys the treated substrates to roller tray End Figure 3.3 PLC programming flow chart Inject again 16 CHAPTER 4 RESULTS AND DISCUSSIONS 4.1 Introduction Results of the automated ozone injection machine project will be discussed in this chapter. There were several processes needed to be done in completing this project. Hence, this chapter will cover all the processes which are prototype designing, controller developing and also animation developing. 4.2 Prototype Design In this section, it will discuss about the results obtained for the prototype design. The prototype of the automated injection machine consists of three main components which are the conveyor, the injection panel and the substrates mold. Apart from that, there are some other components which are the ozone generator to generate ozone, an ozone tank to store generated ozone before injection process is perform and also a monitoring unit to monitor the flow of the machine’s operation. All the designed components have their own dimensions. However, in the future, the dimension can be changed if there are any changes in the design. All the designs of the component were obtained by using SolidWorks software. 17 4.2.1 Conveyor The design of the conveyor consists of four stands for stability purpose. Then, it will be moved by using two motors attached at both ends of the conveyor. A roller tray was connected at one end of the conveyor. The roller tray functions as a temporary holder of the treated substrates before it was lifted by the workers. The dimensions of components in the conveyor were as follows. However, the dimensions can be edited if there are any changes to be made. Figure 4.1 below illustrates the conveyor in multiple views which are from front, left, top and trimetric view. Motor Stands (a) Conveyor Tray (c) Figure 4.1 (b) (d) The conveyor from (a) front (b) left (c) top and (d) trimetric views 18 4.2.2 Injection Panel The injection panel contains series of needles to inject the mushroom substrate with ozone for sterilization purpose. Each injection panel consists of 81 needles. Since an automated treatment machine was designed to have four panels of injection, it means that the machine contains of 324 needles. Hence, for each treatment session ,a large scale of substrate can be injected. Figure 4.2 and Figure 4.2 show the injection tools in a clearer view. (a) Figure 4.2 Injection panel in (a) trimetric and (b) top views Figure 4.3 Series of needles in the panel (b) 19 The injection panel was hold by a holder. The holder of the injection panel adapted the concept of pneumatic. The holder can move up and down during the injection process. This feature helps to make the treatment process smoother. Figure 4.4 below illustrates the holder in multiple views with its dimensions. (a) (b) Figure 4.4 (c) (d) Injection panel’s holder in multiple views; (a) front (b) top (c) left and (d) trimetric views 4.2.3 Substrates Mold The base or the substrates mold was designed with containing series of “cups”. The mushroom substrates were placed in the cups of the mold during the injection session. The function of the cups is to prevent the wastage of ozone. The cups will act as a covered mug during the injection process. As a result, the injected ozone will be totally absorbed by the substrates without any waste. Figure 4.5 below shows the substrates mold clearly with its dimension in various views. 20 (a) (b) Series of “cups” (c) Figure 4.5 (d) The substrates mold from (a) front (b) diametric (c) top and (d) trimetric views 4.2.4 Ozone Generator This machine was attached with an ozone generator that generates the ozone for the treatment process. The generator was build inside a generator board for safety purpose. It is because ozone generator is one of the high voltage equipment. Figure 4.6 shows the ozone generator board in multiple views. 21 Figure 4.6 4.2.5 (a) (b) (c) (d) Ozone generator in (a) front (b) left (c) right and (d) trimetric views Ozone Tank An ozone tank is shown in the Figure 4.7. The purpose of this tank is to store the ozone that has been generated from the generator. During the injection process, the ozone used was obtained from the tank. 22 (a) (c) Figure 4.7 4.2.6 (b) (d) Ozone tank from (a) front (b) left (c) top and (d) trimetric views Monitoring Unit The operation of this treatment machine was fully automated. Therefore, a monitoring unit was needed to monitor the automation of the machine. The monitoring unit consists of LEDs light, a panel monitor and also push button to start and stop the treatment process. Figure 4.8 illustrates how the monitoring unit looks like. 23 Figure 4.8 4.2.7 (a) (b) (c) (d) Monitoring unit in (a) front (b) top (c) right and (d) isometric views The Machine Figure 4.9 demonstrates the whole system of the machine which integrated all the components described in the section above in isometric view. 24 Ozone tank Ozone generator PLC monitoring unit Substrate “cups” Injection panel Figure 4.9 4.3 Isometric view of the automated treatment machine PLC Controller In this project, in order to automate the operation of the machine, a PLC controller was used. PLC controller suited the system well since the machine was bulky. Before developing the controller, a ladder diagram must first be constructed. By using the constructed ladder diagram, then only the system can be run in OMRON CX-One Programmer to check the validity of the automation process. Figure 4.10 denotes the ladder diagram that has been used in this project. . 25 Figure 4.10 Ladder diagram 4.3.1 The Sequence of the Controller Process The sequence of the controller process can be interpreted based on the ladder diagram shown in the section above. To start up the machine, the operator must push the green button available on the monitoring unit. Once the button was pushed, the machine will be ON and the mass sensor will start sensing if the mushrooms substrates were loaded in the mold. The content of the ozone in the tank must be 26 more than 50% for the process to be continued. Then, when the two conditions have been fulfilled, the first green light will light on. At the same time, the injection panels will move down until it is able to censor the injection sensor on the holder. After that, the process of ozone injection will start and this process was set to be within 30 minutes. During this process, the red LED light will light on. After 30 minutes, the second green light on the monitoring unit will light on. This indicates that the process has been successfully carried out. In the meantime, the injection panel will move upward until it censors the stop sensor. Then, the conveyor and the rollers will start to move to convey the treated substrates. The rollers will stop when it sense the stop sensor at the end of the tray. For every step, there must be an emergency button for safety purpose. If any failure happens during the process, the emergency button will be activated. Once activated, the buzzer starts to chime and the emergency LED light also will start to light on. This situation can only be stopped once the reset button was pushed. 4.3.2 CX-One Programmer Simulation The simulation of this program was demonstrated by the video placed in the CD that was attached together with this thesis. 4.4 Animation In order to express the operation of the ozone treatment system for mushroom sterilization, an animation had been developed. The animation was developed by using SolidWorks software with the aid of Adobe Premier Pro video software. The animation video also was available in the attached CD. 27 CHAPTER 5 CONCLUSION AND RECOMMENDATION 5.1 Conclusion At the end of the study, it can be concluded that a prototype of the new automated ozone treatment machine was managed to be designed by using SolidWorks software. During the designing process, there are several features and specification that need to be taken into account. Next, by using the same software, an animation was successfully developed in order to express the ozone treatment machine’s operation. Then, by using OMRON CX-One programmer, a controller also was successfully constructed for automation purpose. In a nutshell, it can be concluded that this project was successful in order to come out with an automated mushroom substrates treatment machine that can improve the cultivation of mushroom especially oyster mushroom. 5.2 Recommendation There are some improvements that can be conducted in the future to improve this project. As recommendations, hardware of the prototype is suggested to be developed in the future so that it can prove the validity of the system established. Other than that, a payback research should be conducted so that it can prove that the new treatment machine is economical than other present substrate treatments 28 REFERENCES [1] Y.Munirah, M. Rozlini , Y. Siti Mariam(2012). Design and Rules Development of Expert System for Diagnosing Oyster Mushroom Disease. International Conference on Computer & Information Science. Malaysia: IEEE, 286 - 288. [2] http://www.fitday.com/fitness-articles/nutrition/healthy-eating/8-types-ofmushrooms-and-their-health-benefits.html [3] Universiti Teknologi Malaysia (2012). Ozone Treatment System for Spawn Treatment of Mushroom Cultivation. [Brochure]. PM Dr. Zolkafle B. Buntat [4] Mushroom cultivation a profitable industry (2012, April 30). Daily News. p. 1 [5] http://en.wikipedia.org/wiki/Mushroom [6] Sanchez, C. (2004) . Modern Aspects of Mushroom Culture Technology. Applied Microbiology and Biotechnology, 64(6), 756-62. [7] http://en.wikipedia.org/wiki/Edible_mushroom. [8] O. Tajudeen, O. Sikhumbuzo etal. (2012). Effect of Substrate Pre-Treatment Methods On Oyster Mushroom Production. Int. J. Agric. Biol , 14:251-255 [9] K. Nasir, M. Abbas etal. (2011). Impact of Various Sterlization Methods Using Different Substrates for Yield Improvement of Pleurotus Spp. Volume (23), 20-23. [10] A. A. Pathan, M. M. Jiskani, M. A. Pathan, K. H. Wagan and Z. A. Nizamani (2009). Effect of Soaking and Boiling of Substrates on the Growth and Productivity of Oyster Mushroom.Volume(21) pp 1-5 29 [11] W. Hui, L. Zhonghai, G. Jijun (2010). Effects of Ozone Combined with High Oxygen Treatment on Fresh-keeping in Postharvest Strawberry Fruits. Central South University of Forestry and Technology [12] http://www.industrysearch.com.au/Benefits-of-using-SolidWorks/f/9604 [13] Dr. Yashmin Sultana (2007). Sterilization Methods and Principles. Dept. of Pharmaceutics Faculty of Pharmacy New Delhi [14] http://itejb-witech.blogspot.com/2013/05/ozone-treatment-system-forspawn.html [15] http://generasighurabaq.blogspot.com/2010/11/autoclave.html [16] HyungJong Kwon, Byung Sik Kim (2004). Cultivation Modes: Bag Cultivation. MushWorld [17] http://z-farm.blogspot.com/2007/11/mushroom.htm 30 APPENDIX A Automated Machine in Front View 31 APPENDIX B Automated Machine from Right and Left Views (a) Left view (b) Right view